def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# load objects
self.microcontroller = microcontroller.Microcontroller_Simulation()
self.navigationController = core.NavigationController(
self.microcontroller)
self.camera_1 = camera.Camera_Simulation(sn='FW0190110139') # tracking
self.camera_2 = camera.Camera_Simulation(
sn='FU0190090030') # fluorescence
self.configurationManager_1 = core.ConfigurationManager(
filename=str(Path.home()) + "/configurations_tracking.xml")
self.configurationManager_2 = core.ConfigurationManager(
filename=str(Path.home()) + "/configurations_fluorescence.xml")
self.streamHandler_1 = core.StreamHandler()
self.liveController_1 = core.LiveController(
self.camera_1,
self.microcontroller,
self.configurationManager_1,
control_illumination=False)
#self.autofocusControlle_1 = core.AutoFocusController(self.camera,self.navigationController,self.liveController)
#self.multipointController_1 = core.MultiPointController(self.camera,self.navigationController,self.liveController,self.autofocusController,self.configurationManager)
self.imageSaver_1 = core.ImageSaver()
self.streamHandler_2 = core.StreamHandler()
self.liveController_2 = core.LiveController(
self.camera_2,
self.microcontroller,
self.configurationManager_2,
control_illumination=True)
self.autofocusController_2 = core.AutoFocusController(
self.camera_2, self.navigationController, self.liveController_2)
self.multipointController_2 = core.MultiPointController(
self.camera_2, self.navigationController, self.liveController_2,
self.autofocusController_2, self.configurationManager_2)
self.imageSaver_2 = core.ImageSaver()
self.trackingController = core.TrackingController(
self.microcontroller, self.navigationController)
# open the camera
# camera start streaming
self.camera_1.open()
self.camera_1.set_software_triggered_acquisition(
) #self.camera.set_continuous_acquisition()
self.camera_1.set_callback(self.streamHandler_1.on_new_frame)
self.camera_1.enable_callback()
self.camera_2.open()
self.camera_2.set_software_triggered_acquisition(
) #self.camera.set_continuous_acquisition()
self.camera_2.set_callback(self.streamHandler_2.on_new_frame)
self.camera_2.enable_callback()
# load widgets
self.navigationWidget = widgets.NavigationWidget(
self.navigationController)
self.cameraSettingWidget_1 = widgets.CameraSettingsWidget(
self.camera_1, self.liveController_1)
self.liveControlWidget_1 = widgets.LiveControlWidget(
self.streamHandler_1, self.liveController_1,
self.configurationManager_1)
self.recordingControlWidget_1 = widgets.RecordingWidget(
self.streamHandler_1, self.imageSaver_1)
#self.trackingControlWidget = widgets.TrackingControllerWidget(self.streamHandler_1,self.trackingController)
self.cameraSettingWidget_2 = widgets.CameraSettingsWidget(
self.camera_2, self.liveController_2)
self.liveControlWidget_2 = widgets.LiveControlWidget(
self.streamHandler_2, self.liveController_2,
self.configurationManager_2)
#self.recordingControlWidget_2 = widgets.RecordingWidget(self.streamHandler_2,self.imageSaver_2)
self.multiPointWidget_2 = widgets.MultiPointWidget(
self.multipointController_2, self.configurationManager_2)
# layout widgets
layout = QGridLayout() #layout = QStackedLayout()
layout.addWidget(self.cameraSettingWidget_1, 0, 0)
layout.addWidget(self.liveControlWidget_1, 1, 0)
layout.addWidget(self.navigationWidget, 2, 0)
#layout.addWidget(self.autofocusWidget,3,0)
layout.addWidget(self.recordingControlWidget_1, 4, 0)
layout.addWidget(self.cameraSettingWidget_2, 5, 0)
layout.addWidget(self.liveControlWidget_2, 6, 0)
#layout.addWidget(self.recordingControlWidget_2,7,0)
layout.addWidget(self.multiPointWidget_2, 8, 0)
# transfer the layout to the central widget
self.centralWidget = QWidget()
self.centralWidget.setLayout(layout)
self.setCentralWidget(self.centralWidget)
# load window
self.imageDisplayWindow_1 = core.ImageDisplayWindow('Tracking')
self.imageDisplayWindow_1.show()
self.imageDisplayWindow_2 = core.ImageDisplayWindow('Fluorescence')
self.imageDisplayWindow_2.show()
self.imageArrayDisplayWindow = core.ImageArrayDisplayWindow(
'Multi-channel')
self.imageArrayDisplayWindow.show()
# make connections
self.navigationController.xPos.connect(
self.navigationWidget.label_Xpos.setNum)
self.navigationController.yPos.connect(
self.navigationWidget.label_Ypos.setNum)
self.navigationController.zPos.connect(
self.navigationWidget.label_Zpos.setNum)
self.streamHandler_1.signal_new_frame_received.connect(
self.liveController_1.on_new_frame)
self.streamHandler_1.image_to_display.connect(
self.imageDisplayWindow_1.display_image)
self.streamHandler_1.packet_image_to_write.connect(
self.imageSaver_1.enqueue)
#self.streamHandler_1.packet_image_for_tracking.connect(self.trackingController.on_new_frame)
self.liveControlWidget_1.signal_newExposureTime.connect(
self.cameraSettingWidget_1.set_exposure_time)
self.liveControlWidget_1.signal_newAnalogGain.connect(
self.cameraSettingWidget_1.set_analog_gain)
self.liveControlWidget_1.update_camera_settings()
self.streamHandler_2.signal_new_frame_received.connect(
self.liveController_2.on_new_frame)
self.streamHandler_2.image_to_display.connect(
self.imageDisplayWindow_2.display_image)
self.streamHandler_2.packet_image_to_write.connect(
self.imageSaver_2.enqueue)
self.liveControlWidget_2.signal_newExposureTime.connect(
self.cameraSettingWidget_2.set_exposure_time)
self.liveControlWidget_2.signal_newAnalogGain.connect(
self.cameraSettingWidget_2.set_analog_gain)
self.liveControlWidget_2.update_camera_settings()
self.multipointController_2.image_to_display.connect(
self.imageDisplayWindow_2.display_image)
self.multipointController_2.image_to_display_multi.connect(
self.imageArrayDisplayWindow.display_image)
self.multipointController_2.signal_current_configuration.connect(
self.liveControlWidget_2.set_microscope_mode)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# load objects
self.camera = camera.Camera_Simulation()
self.microcontroller = microcontroller.Microcontroller_Simulation()
self.streamHandler = core.StreamHandler()
self.liveController = core.LiveController(self.camera,
self.microcontroller)
self.navigationController = core.NavigationController(
self.microcontroller)
self.autofocusController = core.AutoFocusController(
self.camera, self.navigationController, self.liveController)
self.multipointController = core.MultiPointController(
self.camera, self.navigationController, self.liveController,
self.autofocusController)
self.trackingController = core.TrackingController(
self.microcontroller, self.navigationController)
self.imageSaver = core.ImageSaver()
self.imageDisplay = core.ImageDisplay()
'''
# thread
self.thread_multiPoint = QThread()
self.thread_multiPoint.start()
self.multipointController.moveToThread(self.thread_multiPoint)
'''
# open the camera
# camera start streaming
self.camera.open()
self.camera.set_software_triggered_acquisition(
) #self.camera.set_continuous_acquisition()
self.camera.set_callback(self.streamHandler.on_new_frame)
self.camera.enable_callback()
# load widgets
self.cameraSettingWidget = widgets.CameraSettingsWidget(
self.camera, self.liveController)
self.liveControlWidget = widgets.LiveControlWidget(
self.streamHandler, self.liveController)
self.navigationWidget = widgets.NavigationWidget(
self.navigationController)
self.autofocusWidget = widgets.AutoFocusWidget(
self.autofocusController)
self.recordingControlWidget = widgets.RecordingWidget(
self.streamHandler, self.imageSaver)
self.trackingControlWidget = widgets.TrackingControllerWidget(
self.streamHandler, self.trackingController)
self.multiPointWidget = widgets.MultiPointWidget(
self.multipointController)
self.recordTabWidget = QTabWidget()
self.recordTabWidget.addTab(self.recordingControlWidget,
"Simple Recording")
self.recordTabWidget.addTab(self.trackingControlWidget, "Tracking")
self.recordTabWidget.addTab(self.multiPointWidget,
"Multipoint Acquisition")
# layout widgets
layout = QGridLayout() #layout = QStackedLayout()
layout.addWidget(self.cameraSettingWidget, 0, 0)
layout.addWidget(self.liveControlWidget, 1, 0)
layout.addWidget(self.navigationWidget, 2, 0)
layout.addWidget(self.autofocusWidget, 3, 0)
layout.addWidget(self.recordTabWidget, 4, 0)
# transfer the layout to the central widget
self.centralWidget = QWidget()
self.centralWidget.setLayout(layout)
self.setCentralWidget(self.centralWidget)
# load window
self.imageDisplayWindow = core.ImageDisplayWindow()
self.imageDisplayWindow.show()
# make connections
self.streamHandler.signal_new_frame_received.connect(
self.liveController.on_new_frame)
self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
self.streamHandler.packet_image_to_write.connect(
self.imageSaver.enqueue)
self.streamHandler.packet_image_for_tracking.connect(
self.trackingController.on_new_frame)
self.imageDisplay.image_to_display.connect(
self.imageDisplayWindow.display_image
) # may connect streamHandler directly to imageDisplayWindow
self.navigationController.xPos.connect(
self.navigationWidget.label_Xpos.setNum)
self.navigationController.yPos.connect(
self.navigationWidget.label_Ypos.setNum)
self.navigationController.zPos.connect(
self.navigationWidget.label_Zpos.setNum)
self.autofocusController.image_to_display.connect(
self.imageDisplayWindow.display_image)
self.multipointController.image_to_display.connect(
self.imageDisplayWindow.display_image)
self.camera.start_streaming()
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# load objects
self.camera = camera.Camera()
self.microcontroller = microcontroller.Microcontroller_Simulation()
self.configurationManager = core.ConfigurationManager()
self.streamHandler = core_volumetric_imaging.StreamHandler(
crop_width=500, crop_height=500)
self.liveController = core.LiveController(self.camera,
self.microcontroller,
self.configurationManager)
self.navigationController = core.NavigationController(
self.microcontroller)
self.autofocusController = core.AutoFocusController(
self.camera, self.navigationController, self.liveController)
self.multipointController = core.MultiPointController(
self.camera, self.navigationController, self.liveController,
self.autofocusController, self.configurationManager)
self.trackingController = core.TrackingController(
self.microcontroller, self.navigationController)
self.imageSaver = core.ImageSaver()
self.imageDisplay = core.ImageDisplay()
# open the camera
# camera start streaming
self.camera.open()
self.camera.set_software_triggered_acquisition(
) #self.camera.set_continuous_acquisition()
self.camera.set_callback(self.streamHandler.on_new_frame)
self.camera.enable_callback()
# load widgets
self.cameraSettingWidget = widgets.CameraSettingsWidget(
self.camera, self.liveController)
self.liveControlWidget = widgets.LiveControlWidget(
self.streamHandler, self.liveController, self.configurationManager)
self.navigationWidget = widgets.NavigationWidget(
self.navigationController)
self.autofocusWidget = widgets.AutoFocusWidget(
self.autofocusController)
self.recordingControlWidget = widgets.RecordingWidget(
self.streamHandler, self.imageSaver)
self.trackingControlWidget = widgets.TrackingControllerWidget(
self.streamHandler, self.trackingController)
self.multiPointWidget = widgets.MultiPointWidget(
self.multipointController, self.configurationManager)
self.recordTabWidget = QTabWidget()
self.recordTabWidget.addTab(self.recordingControlWidget,
"Simple Recording")
# self.recordTabWidget.addTab(self.trackingControlWidget, "Tracking")
# self.recordTabWidget.addTab(self.multiPointWidget, "Multipoint Acquisition")
# layout widgets
layout = QGridLayout() #layout = QStackedLayout()
layout.addWidget(self.cameraSettingWidget, 0, 0)
layout.addWidget(self.liveControlWidget, 1, 0)
# layout.addWidget(self.navigationWidget,2,0)
# layout.addWidget(self.autofocusWidget,3,0)
layout.addWidget(self.recordTabWidget, 4, 0)
# transfer the layout to the central widget
self.centralWidget = QWidget()
self.centralWidget.setLayout(layout)
self.setCentralWidget(self.centralWidget)
# load window
self.imageDisplayWindow = core.ImageDisplayWindow()
self.imageArrayDisplayWindow = core_volumetric_imaging.ImageArrayDisplayWindow(
)
self.imageDisplayWindow.show()
self.imageArrayDisplayWindow.show()
# make connections
self.streamHandler.signal_new_frame_received.connect(
self.liveController.on_new_frame)
self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
self.streamHandler.packet_image_to_write.connect(
self.imageSaver.enqueue)
self.streamHandler.packet_image_for_tracking.connect(
self.trackingController.on_new_frame)
self.streamHandler.packet_image_for_array_display.connect(
self.imageArrayDisplayWindow.display_image)
self.imageDisplay.image_to_display.connect(
self.imageDisplayWindow.display_image
) # may connect streamHandler directly to imageDisplayWindow
self.navigationController.xPos.connect(
self.navigationWidget.label_Xpos.setNum)
self.navigationController.yPos.connect(
self.navigationWidget.label_Ypos.setNum)
self.navigationController.zPos.connect(
self.navigationWidget.label_Zpos.setNum)
self.autofocusController.image_to_display.connect(
self.imageDisplayWindow.display_image)
# self.multipointController.image_to_display.connect(self.imageDisplayWindow.display_image)
self.multipointController.signal_current_configuration.connect(
self.liveControlWidget.set_microscope_mode)
self.multipointController.image_to_display_multi.connect(
self.imageArrayDisplayWindow.display_image)
self.liveControlWidget.signal_newExposureTime.connect(
self.cameraSettingWidget.set_exposure_time)
self.liveControlWidget.signal_newAnalogGain.connect(
self.cameraSettingWidget.set_analog_gain)
self.liveControlWidget.update_camera_settings()
def __init__(self, is_simulation=False, *args, **kwargs):
super().__init__(*args, **kwargs)
# load window
if ENABLE_TRACKING:
self.imageDisplayWindow = core.ImageDisplayWindow(
draw_crosshairs=True)
self.imageDisplayWindow.show_ROI_selector()
else:
self.imageDisplayWindow = core.ImageDisplayWindow(
draw_crosshairs=True)
self.imageArrayDisplayWindow = core.ImageArrayDisplayWindow()
# self.imageDisplayWindow.show()
# self.imageArrayDisplayWindow.show()
# image display windows
self.imageDisplayTabs = QTabWidget()
self.imageDisplayTabs.addTab(self.imageDisplayWindow.widget,
"Live View")
self.imageDisplayTabs.addTab(self.imageArrayDisplayWindow.widget,
"Multichannel Acquisition")
# load objects
if is_simulation:
self.camera = camera.Camera_Simulation(
rotate_image_angle=ROTATE_IMAGE_ANGLE, flip_image=FLIP_IMAGE)
self.microcontroller = microcontroller.Microcontroller_Simulation()
else:
self.camera = camera.Camera(rotate_image_angle=ROTATE_IMAGE_ANGLE,
flip_image=FLIP_IMAGE)
self.microcontroller = microcontroller.Microcontroller()
# configure the actuators
self.microcontroller.configure_actuators()
self.configurationManager = core.ConfigurationManager()
self.streamHandler = core.StreamHandler(
display_resolution_scaling=DEFAULT_DISPLAY_CROP / 100)
self.liveController = core.LiveController(self.camera,
self.microcontroller,
self.configurationManager)
self.navigationController = core.NavigationController(
self.microcontroller)
self.autofocusController = core.AutoFocusController(
self.camera, self.navigationController, self.liveController)
self.multipointController = core.MultiPointController(
self.camera, self.navigationController, self.liveController,
self.autofocusController, self.configurationManager)
if ENABLE_TRACKING:
self.trackingController = core.TrackingController(
self.camera, self.microcontroller, self.navigationController,
self.configurationManager, self.liveController,
self.autofocusController, self.imageDisplayWindow)
self.imageSaver = core.ImageSaver(
image_format=Acquisition.IMAGE_FORMAT)
self.imageDisplay = core.ImageDisplay()
# open the camera
# camera start streaming
self.camera.open()
# self.camera.set_reverse_x(CAMERA_REVERSE_X) # these are not implemented for the cameras in use
# self.camera.set_reverse_y(CAMERA_REVERSE_Y) # these are not implemented for the cameras in use
self.camera.set_software_triggered_acquisition(
) #self.camera.set_continuous_acquisition()
self.camera.set_callback(self.streamHandler.on_new_frame)
self.camera.enable_callback()
# load widgets
self.cameraSettingWidget = widgets.CameraSettingsWidget(
self.camera, include_gain_exposure_time=False)
self.liveControlWidget = widgets.LiveControlWidget(
self.streamHandler, self.liveController, self.configurationManager)
self.navigationWidget = widgets.NavigationWidget(
self.navigationController)
self.dacControlWidget = widgets.DACControWidget(self.microcontroller)
self.autofocusWidget = widgets.AutoFocusWidget(
self.autofocusController)
self.recordingControlWidget = widgets.RecordingWidget(
self.streamHandler, self.imageSaver)
if ENABLE_TRACKING:
self.trackingControlWidget = widgets.TrackingControllerWidget(
self.trackingController,
self.configurationManager,
show_configurations=TRACKING_SHOW_MICROSCOPE_CONFIGURATIONS)
self.multiPointWidget = widgets.MultiPointWidget(
self.multipointController, self.configurationManager)
self.recordTabWidget = QTabWidget()
if ENABLE_TRACKING:
self.recordTabWidget.addTab(self.trackingControlWidget, "Tracking")
self.recordTabWidget.addTab(self.recordingControlWidget,
"Simple Recording")
self.recordTabWidget.addTab(self.multiPointWidget,
"Multipoint Acquisition")
# layout widgets
layout = QVBoxLayout() #layout = QStackedLayout()
layout.addWidget(self.cameraSettingWidget)
layout.addWidget(self.liveControlWidget)
layout.addWidget(self.navigationWidget)
if SHOW_DAC_CONTROL:
layout.addWidget(self.dacControlWidget)
layout.addWidget(self.autofocusWidget)
layout.addWidget(self.recordTabWidget)
layout.addStretch()
# transfer the layout to the central widget
self.centralWidget = QWidget()
self.centralWidget.setLayout(layout)
# self.centralWidget.setFixedSize(self.centralWidget.minimumSize())
# self.centralWidget.setFixedWidth(self.centralWidget.minimumWidth())
# self.centralWidget.setMaximumWidth(self.centralWidget.minimumWidth())
self.centralWidget.setFixedWidth(
self.centralWidget.minimumSizeHint().width())
if SINGLE_WINDOW:
dock_display = dock.Dock('Image Display', autoOrientation=False)
dock_display.showTitleBar()
dock_display.addWidget(self.imageDisplayTabs)
dock_display.setStretch(x=100, y=None)
dock_controlPanel = dock.Dock('Controls', autoOrientation=False)
# dock_controlPanel.showTitleBar()
dock_controlPanel.addWidget(self.centralWidget)
dock_controlPanel.setStretch(x=1, y=None)
dock_controlPanel.setFixedWidth(
dock_controlPanel.minimumSizeHint().width())
main_dockArea = dock.DockArea()
main_dockArea.addDock(dock_display)
main_dockArea.addDock(dock_controlPanel, 'right')
self.setCentralWidget(main_dockArea)
desktopWidget = QDesktopWidget()
height_min = 0.9 * desktopWidget.height()
width_min = 0.96 * desktopWidget.width()
self.setMinimumSize(width_min, height_min)
else:
self.setCentralWidget(self.centralWidget)
self.tabbedImageDisplayWindow = QMainWindow()
self.tabbedImageDisplayWindow.setCentralWidget(
self.imageDisplayTabs)
self.tabbedImageDisplayWindow.setWindowFlags(
self.windowFlags() | Qt.CustomizeWindowHint)
self.tabbedImageDisplayWindow.setWindowFlags(
self.windowFlags() & ~Qt.WindowCloseButtonHint)
desktopWidget = QDesktopWidget()
width = 0.96 * desktopWidget.height()
height = width
self.tabbedImageDisplayWindow.setFixedSize(width, height)
self.tabbedImageDisplayWindow.show()
# make connections
self.streamHandler.signal_new_frame_received.connect(
self.liveController.on_new_frame)
self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
self.streamHandler.packet_image_to_write.connect(
self.imageSaver.enqueue)
# self.streamHandler.packet_image_for_tracking.connect(self.trackingController.on_new_frame)
self.imageDisplay.image_to_display.connect(
self.imageDisplayWindow.display_image
) # may connect streamHandler directly to imageDisplayWindow
self.navigationController.xPos.connect(
self.navigationWidget.label_Xpos.setNum)
self.navigationController.yPos.connect(
self.navigationWidget.label_Ypos.setNum)
self.navigationController.zPos.connect(
self.navigationWidget.label_Zpos.setNum)
if ENABLE_TRACKING:
self.navigationController.signal_joystick_button_pressed.connect(
self.trackingControlWidget.slot_joystick_button_pressed)
else:
self.navigationController.signal_joystick_button_pressed.connect(
self.autofocusController.autofocus)
self.autofocusController.image_to_display.connect(
self.imageDisplayWindow.display_image)
self.multipointController.image_to_display.connect(
self.imageDisplayWindow.display_image)
self.multipointController.signal_current_configuration.connect(
self.liveControlWidget.set_microscope_mode)
self.multipointController.image_to_display_multi.connect(
self.imageArrayDisplayWindow.display_image)
self.liveControlWidget.signal_newExposureTime.connect(
self.cameraSettingWidget.set_exposure_time)
self.liveControlWidget.signal_newAnalogGain.connect(
self.cameraSettingWidget.set_analog_gain)
self.liveControlWidget.update_camera_settings()