def reconst_gqi_fodf_return_sh_coeffs(bval_path,bvec_path,data_path,data_var):
bval_path = os.path.normpath(bval_path)
bvec_path = os.path.normpath(bvec_path)
data_path = os.path.normpath(data_path)
bvals, bvecs = read_bvals_bvecs(bval_path, bvec_path)
gtab = gradient_table(bvals, bvecs)
data = loadmat(data_path)
data = data[data_var]
gqmodel = GeneralizedQSamplingModel(gtab, sampling_length=1.2)
#gqfit = gqmodel.fit(dataslice, mask=mask)
sphere = get_sphere('symmetric724')
#ODF = gqfit.odf(sphere)
#odf = gqmodel.fit(data).odf(sphere)
gqpeaks = peaks_from_model(model=gqmodel,
data=data,
sphere=sphere,
relative_peak_threshold=.5,
min_separation_angle=25,
return_odf=True,
normalize_peaks=True)
print('Debug here')
fodfs_shm = gqpeaks.shm_coeff
return fodfs_shm
def dipy_gt():
print("Loading slice...")
sample_slice, qvecs, gtab = load_slice('./test_data/david_data', '3112_BL_data_subject_space.nii.gz', gtab=True)
mapmodel = GeneralizedQSamplingModel(gtab)
mapfit = mapmodel.fit(sample_slice)
sphere = get_sphere('repulsion724')
odfs = mapfit.odf(sphere)
vis_2d_field(odfs, sphere)
def test_mvoxel_gqi():
data, gtab = dsi_voxels()
sphere = get_sphere('symmetric724')
gq = GeneralizedQSamplingModel(gtab, 'standard')
gqfit = gq.fit(data)
all_odfs = gqfit.odf(sphere)
# Check that the first and last voxels each have 2 peaks
odf = all_odfs[0, 0, 0]
directions, values, indices = peak_directions(odf, sphere, .35, 25)
assert_equal(directions.shape[0], 2)
odf = all_odfs[-1, -1, -1]
directions, values, indices = peak_directions(odf, sphere, .35, 25)
assert_equal(directions.shape[0], 2)
def test_gqi():
#load symmetric 724 sphere
sphere = get_sphere('symmetric724')
#load icosahedron sphere
sphere2 = create_unit_sphere(5)
btable = np.loadtxt(get_data('dsi515btable'))
bvals = btable[:, 0]
bvecs = btable[:, 1:]
gtab = gradient_table(bvals, bvecs)
data, golden_directions = SticksAndBall(gtab,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0)],
fractions=[50, 50],
snr=None)
gq = GeneralizedQSamplingModel(gtab, method='gqi2', sampling_length=1.4)
#symmetric724
gqfit = gq.fit(data)
odf = gqfit.odf(sphere)
directions, values, indices = peak_directions(odf, sphere, .35, 25)
assert_equal(len(directions), 2)
assert_almost_equal(angular_similarity(directions, golden_directions), 2,
1)
#5 subdivisions
gqfit = gq.fit(data)
odf2 = gqfit.odf(sphere2)
directions, values, indices = peak_directions(odf2, sphere2, .35, 25)
assert_equal(len(directions), 2)
assert_almost_equal(angular_similarity(directions, golden_directions), 2,
1)
sb_dummies = sticks_and_ball_dummies(gtab)
for sbd in sb_dummies:
data, golden_directions = sb_dummies[sbd]
odf = gq.fit(data).odf(sphere2)
directions, values, indices = peak_directions(odf, sphere2, .35, 25)
if len(directions) <= 3:
assert_equal(len(directions), len(golden_directions))
if len(directions) > 3:
assert_equal(gfa(odf) < 0.1, True)
data.shape ``(96, 96, 60, 203)`` This dataset has anisotropic voxel sizes, therefore reslicing is necessary. """ affine = img.affine """ Read the voxel size from the image header. """ voxel_size = img.header.get_zooms()[:3] """ Instantiate the model and apply it to the data. """ gqmodel = GeneralizedQSamplingModel(gtab, sampling_length=3) """ The parameter ``sampling_length`` is used here to Lets just use one slice only from the data. """ dataslice = data[:, :, data.shape[2] // 2] mask = dataslice[..., 0] > 50 gqfit = gqmodel.fit(dataslice, mask=mask) """ Load an ODF reconstruction sphere """
def dmri_recon(sid,
data_dir,
out_dir,
resolution,
recon='csd',
dirs='',
num_threads=2):
import tempfile
#tempfile.tempdir = '/om/scratch/Fri/ksitek/'
import os
oldval = None
if 'MKL_NUM_THREADS' in os.environ:
oldval = os.environ['MKL_NUM_THREADS']
os.environ['MKL_NUM_THREADS'] = '%d' % num_threads
ompoldval = None
if 'OMP_NUM_THREADS' in os.environ:
ompoldval = os.environ['OMP_NUM_THREADS']
os.environ['OMP_NUM_THREADS'] = '%d' % num_threads
import nibabel as nib
import numpy as np
from glob import glob
if resolution == '0.2mm':
filename = 'Reg_S64550_nii4d.nii'
#filename = 'angular_resample/dwi_%s.nii.gz'%dirs
fimg = os.path.abspath(glob(os.path.join(data_dir, filename))[0])
else:
filename = 'Reg_S64550_nii4d_resamp-%s.nii.gz' % (resolution)
fimg = os.path.abspath(
glob(os.path.join(data_dir, 'resample', filename))[0])
print("dwi file = %s" % fimg)
fbval = os.path.abspath(
glob(os.path.join(data_dir, 'bvecs', 'camino_120_RAS.bvals'))[0])
print("bval file = %s" % fbval)
fbvec = os.path.abspath(
glob(os.path.join(data_dir, 'bvecs',
'camino_120_RAS_flipped-xy.bvecs'))[0])
# 'angular_resample',
# 'dwi_%s.bvecs'%dirs))[0])
print("bvec file = %s" % fbvec)
img = nib.load(fimg)
data = img.get_fdata()
affine = img.get_affine()
prefix = sid
from dipy.io import read_bvals_bvecs
bvals, bvecs = read_bvals_bvecs(fbval, fbvec)
'''
from dipy.core.gradients import vector_norm
b0idx = []
for idx, val in enumerate(bvals):
if val < 1:
pass
#bvecs[idx] = [1, 0, 0]
else:
b0idx.append(idx)
#print "b0idx=%d"%idx
#print "input bvecs:"
#print bvecs
bvecs[b0idx, :] = bvecs[b0idx, :]/vector_norm(bvecs[b0idx])[:, None]
#print "bvecs after normalization:"
#print bvecs
'''
from dipy.core.gradients import gradient_table
gtab = gradient_table(bvals, bvecs)
gtab.bvecs.shape == bvecs.shape
gtab.bvecs
gtab.bvals.shape == bvals.shape
gtab.bvals
#from dipy.segment.mask import median_otsu
#b0_mask, mask = median_otsu(data[:, :, :, b0idx].mean(axis=3).squeeze(), 4, 4)
if resolution == '0.2mm':
mask_name = 'Reg_S64550_nii_b0-slice_mask.nii.gz'
fmask1 = os.path.join(data_dir, mask_name)
else:
mask_name = 'Reg_S64550_nii_b0-slice_mask_resamp-%s.nii.gz' % (
resolution)
fmask1 = os.path.join(data_dir, 'resample', mask_name)
print("fmask file = %s" % fmask1)
mask = nib.load(fmask1).get_fdata()
''' DTI model & save metrics '''
from dipy.reconst.dti import TensorModel
print("running tensor model")
tenmodel = TensorModel(gtab)
tenfit = tenmodel.fit(data, mask)
from dipy.reconst.dti import fractional_anisotropy
print("running FA")
FA = fractional_anisotropy(tenfit.evals)
FA[np.isnan(FA)] = 0
fa_img = nib.Nifti1Image(FA, img.get_affine())
tensor_fa_file = os.path.abspath('%s_tensor_fa.nii.gz' % (prefix))
nib.save(fa_img, tensor_fa_file)
from dipy.reconst.dti import axial_diffusivity
print("running AD")
AD = axial_diffusivity(tenfit.evals)
AD[np.isnan(AD)] = 0
ad_img = nib.Nifti1Image(AD, img.get_affine())
tensor_ad_file = os.path.abspath('%s_tensor_ad.nii.gz' % (prefix))
nib.save(ad_img, tensor_ad_file)
from dipy.reconst.dti import radial_diffusivity
print("running RD")
RD = radial_diffusivity(tenfit.evals)
RD[np.isnan(RD)] = 0
rd_img = nib.Nifti1Image(RD, img.get_affine())
tensor_rd_file = os.path.abspath('%s_tensor_rd.nii.gz' % (prefix))
nib.save(rd_img, tensor_rd_file)
from dipy.reconst.dti import mean_diffusivity
print("running MD")
MD = mean_diffusivity(tenfit.evals)
MD[np.isnan(MD)] = 0
md_img = nib.Nifti1Image(MD, img.get_affine())
tensor_md_file = os.path.abspath('%s_tensor_md.nii.gz' % (prefix))
nib.save(md_img, tensor_md_file)
evecs = tenfit.evecs
evec_img = nib.Nifti1Image(evecs, img.get_affine())
tensor_evec_file = os.path.abspath('%s_tensor_evec.nii.gz' % (prefix))
nib.save(evec_img, tensor_evec_file)
''' ODF model '''
useFA = True
print("creating %s model" % recon)
if recon == 'csd':
from dipy.reconst.csdeconv import ConstrainedSphericalDeconvModel
from dipy.reconst.csdeconv import auto_response
response, ratio = auto_response(gtab, data, roi_radius=10,
fa_thr=0.5) # 0.7
model = ConstrainedSphericalDeconvModel(gtab, response)
useFA = True
return_sh = True
elif recon == 'csa':
from dipy.reconst.shm import CsaOdfModel, normalize_data
model = CsaOdfModel(gtab, sh_order=8)
useFA = True
return_sh = True
elif recon == 'gqi':
from dipy.reconst.gqi import GeneralizedQSamplingModel
model = GeneralizedQSamplingModel(gtab)
return_sh = False
else:
raise ValueError('only csd, csa supported currently')
from dipy.reconst.dsi import (DiffusionSpectrumDeconvModel,
DiffusionSpectrumModel)
model = DiffusionSpectrumDeconvModel(gtab)
'''reconstruct ODFs'''
from dipy.data import get_sphere
sphere = get_sphere('symmetric724')
#odfs = fit.odf(sphere)
# with CSD/GQI, uses > 50GB per core; don't get greedy with cores!
from dipy.reconst.peaks import peaks_from_model
print("running peaks_from_model")
peaks = peaks_from_model(
model=model,
data=data,
sphere=sphere,
mask=mask,
return_sh=return_sh,
return_odf=False,
normalize_peaks=True,
npeaks=5,
relative_peak_threshold=.5,
min_separation_angle=10, #25,
parallel=num_threads > 1,
nbr_processes=num_threads)
# save the peaks
from dipy.io.peaks import save_peaks
peaks_file = os.path.abspath('%s_peaks.pam5' % (prefix))
save_peaks(peaks_file, peaks)
# save the spherical harmonics
shm_coeff_file = os.path.abspath('%s_shm_coeff.nii.gz' % (prefix))
if return_sh:
shm_coeff = peaks.shm_coeff
nib.save(nib.Nifti1Image(shm_coeff, img.get_affine()), shm_coeff_file)
else:
# if it's not a spherical model, output it as an essentially null file
np.savetxt(shm_coeff_file, [0])
# save the generalized fractional anisotropy image
gfa_img = nib.Nifti1Image(peaks.gfa, img.get_affine())
model_gfa_file = os.path.abspath('%s_%s_gfa.nii.gz' % (prefix, recon))
nib.save(gfa_img, model_gfa_file)
#from dipy.reconst.dti import quantize_evecs
#peak_indices = quantize_evecs(tenfit.evecs, sphere.vertices)
#eu = EuDX(FA, peak_indices, odf_vertices = sphere.vertices,
#a_low=0.2, seeds=10**6, ang_thr=35)
''' probabilistic tracking '''
'''
from dipy.direction import ProbabilisticDirectionGetter
from dipy.tracking.local import LocalTracking
from dipy.tracking.streamline import Streamlines
from dipy.io.streamline import save_trk
prob_dg = ProbabilisticDirectionGetter.from_shcoeff(shm_coeff,
max_angle=45.,
sphere=sphere)
streamlines_generator = LocalTracking(prob_dg,
affine,
step_size=.5,
max_cross=1)
# Generate streamlines object
streamlines = Streamlines(streamlines_generator)
affine = img.get_affine()
vox_size=fa_img.get_header().get_zooms()[:3]
fname = os.path.abspath('%s_%s_prob_streamline.trk' % (prefix, recon))
save_trk(fname, streamlines, affine, vox_size=vox_size)
'''
''' deterministic tracking with EuDX method'''
from dipy.tracking.eudx import EuDX
print("reconstructing with EuDX")
if useFA:
eu = EuDX(
FA,
peaks.peak_indices[..., 0],
odf_vertices=sphere.vertices,
a_low=0.001, # default is 0.0239
seeds=10**6,
ang_thr=75)
else:
eu = EuDX(
peaks.gfa,
peaks.peak_indices[..., 0],
odf_vertices=sphere.vertices,
#a_low=0.1,
seeds=10**6,
ang_thr=45)
sl_fname = os.path.abspath('%s_%s_det_streamline.trk' % (prefix, recon))
# trying new dipy.io.streamline module, per email to neuroimaging list
# 2018.04.05
from nibabel.streamlines import Field
from nibabel.orientations import aff2axcodes
affine = img.get_affine()
vox_size = fa_img.get_header().get_zooms()[:3]
fov_shape = FA.shape[:3]
if vox_size is not None and fov_shape is not None:
hdr = {}
hdr[Field.VOXEL_TO_RASMM] = affine.copy()
hdr[Field.VOXEL_SIZES] = vox_size
hdr[Field.DIMENSIONS] = fov_shape
hdr[Field.VOXEL_ORDER] = "".join(aff2axcodes(affine))
tractogram = nib.streamlines.Tractogram(eu)
tractogram.affine_to_rasmm = affine
trk_file = nib.streamlines.TrkFile(tractogram, header=hdr)
nib.streamlines.save(trk_file, sl_fname)
if oldval:
os.environ['MKL_NUM_THREADS'] = oldval
else:
del os.environ['MKL_NUM_THREADS']
if ompoldval:
os.environ['OMP_NUM_THREADS'] = ompoldval
else:
del os.environ['OMP_NUM_THREADS']
print('all output files created')
return (tensor_fa_file, tensor_evec_file, model_gfa_file, sl_fname, affine,
tensor_ad_file, tensor_rd_file, tensor_md_file, shm_coeff_file,
peaks_file)
def test_gqi():
#load odf sphere
vertices, faces = sphere_vf_from('symmetric724')
edges = unique_edges(faces)
half_vertices, half_edges, half_faces = reduce_antipodal(vertices, faces)
#load bvals and gradients
btable = np.loadtxt(get_data('dsi515btable'))
bvals = btable[:, 0]
bvecs = btable[:, 1:]
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[50, 50, 0],
snr=None)
#pdf0,odf0,peaks0=standard_dsi_algorithm(S,bvals,bvecs)
S2 = S.copy()
S2 = S2.reshape(1, len(S))
odf_sphere = (vertices, faces)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere)
dsfit = ds.fit(S)
assert_equal((dsfit.peak_values > 0).sum(), 3)
#change thresholds
ds.relative_peak_threshold = 0.5
ds.angular_distance_threshold = 30
dsfit = ds.fit(S)
assert_equal((dsfit.peak_values > 0).sum(), 2)
#1 fiber
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[100, 0, 0],
snr=None)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere)
ds.relative_peak_threshold = 0.5
ds.angular_distance_threshold = 20
dsfit = ds.fit(S)
QA = dsfit.qa
#1/0
assert_equal(np.sum(QA > 0), 1)
#2 fibers
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[50, 50, 0],
snr=None)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere)
ds.relative_peak_threshold = 0.5
ds.angular_distance_threshold = 20
dsfit = ds.fit(S)
QA = dsfit.qa
assert_equal(np.sum(QA > 0), 2)
#3 fibers
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[33, 33, 33],
snr=None)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere)
ds.relative_peak_threshold = 0.5
dsfit = ds.fit(S)
QA = dsfit.qa
assert_equal(np.sum(QA > 0), 3)
#isotropic
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[0, 0, 0],
snr=None)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere)
dsfit = ds.fit(S)
QA = dsfit.qa
assert_equal(np.sum(QA > 0), 0)
#3 fibers DSI2
S, stics = SticksAndBall(bvals,
bvecs,
d=0.0015,
S0=100,
angles=[(0, 0), (90, 0), (90, 90)],
fractions=[33, 33, 33],
snr=None)
ds = GeneralizedQSamplingModel(bvals, bvecs, odf_sphere, squared=True)
ds.relative_peak_threshold = 0.5
dsfit = ds.fit(S, gfa_thr=0.05)
QA = dsfit.qa
#3 fibers DSI2 with a 3D volume
data = np.zeros((3, 3, 3, len(S)))
data[..., :] = S.copy()
dsfit = ds.fit(data, gfa_thr=0.05)
#1/0
assert_array_almost_equal(np.sum(dsfit.peak_values > 0, axis=-1),
3 * np.ones((3, 3, 3)))
