Options:
-v --volume <mL> [default: 40]
Roughly how much stock solution you want to prepare.
-c --conc <mM> [default: 30]
The concentration of theophylline you want to prepare.
Note that theophylline is a drug, and as such it is fairly toxic. Be careful
not to inhale or ingest it and read the MSDS for more information.
"""
import docopt
import dirty_water
args = docopt.docopt(__doc__)
protocol = dirty_water.Protocol()
volume = eval(args['--volume'])
conc = eval(args['--conc'])
mw = 180.16
mg_theo = volume * conc * mw / 1000
mL_water = 1000 / mw / conc
protocol += """\
Measure approximately {mg_theo:.1f} mg theophylline
into a glass bottle with a stir bar."""
protocol += """\
Add {mL_water:.4f} mL water for each mg theophyline."""
protocol += """\
#!/usr/bin/env python3
"""\
Transform an sgRNA library into yeast using the Benatuil electroporation
protocol.
Usage:
yeast_library_prep.py <num_transformations>
"""
import docopt
import dirty_water
from nonstdlib import plural
args = docopt.docopt(__doc__)
N = int(eval(args['<num_transformations>']))
day_1 = dirty_water.Protocol()
day_2 = dirty_water.Protocol()
## Day 1
ypd_mL = 40 * N
overnight_mL = 0.3 * ypd_mL / 10.0
day_1 += f"""\
Prepare 10 81 cm² selective plates [1]."""
sorbitol_mL = N * (115 + 5)
sorbitol_g = sorbitol_mL * 182.17 / 1000
day_1 += f"""\
Prepare {sorbitol_mL} mL 1M sorbitol:
def test_protocol_str_steps():
protocol = dirty_water.Protocol()
protocol += 'hello'
protocol += 'world'
assert str(protocol) == """\
