def _merge_pacbporfs_by_tinyexon_and_two_introns(pacbporfD,pacbporfA,
orfSetObject,queryorsbjct,verbose = False, **kwargs):
"""
Merge 2 PacbPORF objects by introns
@attention: see pacb.connecting.merge_orfs_with_intron for **kwargs)
@type pacbporfD: PacbPORF object
@param pacbporfD: PacbPORF object that has to deliver PSSM donor objects
@type pacbporfA: PacbPORF object
@param pacbporfA: PacbPORF object that has to deliver PSSM acceptor objects
@type orfSetObject: object with elegiable Orfs
@param orfSetObject: object with elegiable Orfs
@type queryorsbjct: string
@param queryorsbjct: literal string 'query' or 'sbjct'
@type verbose: Boolean
@param verbose: print debugging info to STDOUT when True
@rtype: list
@return: list with ( intron, ExonOnOrf, intron ) on the query sequence
"""
# input validation
IsPacbPORF(pacbporfD)
IsPacbPORF(pacbporfA)
# edit **kwargs dictionary for some forced attributes
_update_kwargs(kwargs,KWARGS_PROJECTED_TINYEXON)
MAX_TINYEXON_NT_LENGTH = 33
MIN_TINYEXON_NT_LENGTH = 6
tinyexons = []
if queryorsbjct == "query":
donorOrf = pacbporfD.orfQ
accepOrf = pacbporfA.orfQ
prjctOrf = pacbporfD.orfS
alignedDonorRange = pacbporfD.alignment_dna_range_query()
alignedAccepRange = pacbporfA.alignment_dna_range_query()
elif queryorsbjct == "sbjct":
donorOrf = pacbporfD.orfS
accepOrf = pacbporfA.orfS
prjctOrf = pacbporfD.orfQ
alignedDonorRange = pacbporfD.alignment_dna_range_sbjct()
alignedAccepRange = pacbporfA.alignment_dna_range_sbjct()
else:
message = "'queryorsbjct' (%s), not 'query' or 'sbjct'" % queryorsbjct
raise InproperlyAppliedArgument, message
for dObj in donorOrf._donor_sites:
# do not make a projection OVER the aligned area
if dObj.pos < min(alignedDonorRange): continue
if queryorsbjct == "query":
(dPos,dPhase) = pacbporfD.dnaposition_query(dObj.pos,forced_return=True)
else:
(dPos,dPhase) = pacbporfD.dnaposition_sbjct(dObj.pos,forced_return=True)
try:
algDobj = pacbporfD._positions[dPos]
except IndexError:
# site out of range of PacbPORF -> break
break
for aObj in accepOrf._acceptor_sites:
# do not make a projection OVER the aligned area
if aObj.pos > max(alignedAccepRange): continue
if queryorsbjct == "query":
(aPos,aPhase) = pacbporfA.dnaposition_query(aObj.pos,forced_return=True)
else:
(aPos,aPhase) = pacbporfA.dnaposition_sbjct(aObj.pos,forced_return=True)
try:
algAobj = pacbporfA._positions[aPos]
except IndexError:
# site out of range of PacbPORF -> break
break
if queryorsbjct == "query":
posDsbjct = algDobj.sbjct_dna_start + dPhase
posAsbjct = algAobj.sbjct_dna_start + aPhase
else:
posDsbjct = algDobj.query_dna_start + dPhase
posAsbjct = algAobj.query_dna_start + aPhase
distance = posAsbjct - posDsbjct
if distance >= MAX_TINYEXON_NT_LENGTH:
break
if distance < MIN_TINYEXON_NT_LENGTH:
continue
####################################################
# generate a ScanForMatches pattern file
####################################################
# example pattern: 6...6 AG NNGNNANNANNGN[2,0,0] GT 3...3
query = list(prjctOrf.inputgenomicsequence[posDsbjct:posAsbjct])
# mask all non-phase0 nucleotides to N residues;
# this represents the regularexpression for a specific
# peptide sequence
firstphasepositions = range( 3-dPhase % 3, len(query), 3)
for pos in range(0,len(query)):
if pos not in firstphasepositions:
query[pos] = "N"
# calculate a ~50% mismatch number
mismatches = max([ 0, (len(query) - query.count("N"))/2 ])
# write the pattern to string and subsequently to file
# example pattern: 6...6 AG NNGNNANNANNGN[2,0,0] GT 3...3
if kwargs['allow_non_canonical_donor']:
sfmpat = "%s...%s AG %s[%s,0,0] G (T | C) %s...%s" % (
AUSO,AUSO,"".join(query),mismatches,DDSO,DDSO)
else:
sfmpat = "%s...%s AG %s[%s,0,0] GT %s...%s" % (
AUSO,AUSO,"".join(query),mismatches,DDSO,DDSO)
####################################################
if verbose:
print (pacbporfD.orfQ.id,pacbporfA.orfQ.id),
print distance, dObj, aObj
print sfmpat
####################################################
fname = "sfmpat_tinyexon_%s_%s_%s_%s" % (
donorOrf.id,
accepOrf.id,
posDsbjct,
posAsbjct,
)
fh = open(fname,'w')
fh.write(sfmpat+"\n")
fh.close()
####################################################
# run ScanForMatches
####################################################
command = """echo ">myseq\n%s" | %s %s | tr "[,]" "\t\t#" | """ +\
"""tr -d "\n " | sed "s/>/\\n>/g" | tr "#" "\t" | """ +\
"""awk -F'\t' '{ if (NF==4 && $2>%s && $3<%s) """ +\
"""{ print $1"["$2","$3"]\\n"$4 } }' """
command = command % (
donorOrf.inputgenomicsequence,
EXECUTABLE_SFM,fname,
dObj.pos+(kwargs['min_intron_nt_length']-3),
aObj.pos-(kwargs['min_intron_nt_length']-3) )
co = osPopen(command)
matches = parseFasta(co.readlines())
co.close()
# filter matches for:
# (1) correct donor & acceptor phase
# (2) high enough donor & acceptor site scores
for hdr,seqmatch in matches.iteritems():
startQ,stopQ = [ int(item) for item in hdr.split(":")[1][1:-1].split(",") ]
exonQstart = startQ + AUSO + 2 - 1
exonQstop = stopQ - DDSO - 2
####################################
# get Orf object of tinyexon
####################################
tinyexonorf = None
# select the Orf on which the tinyexon is located
for orfObj in orfSetObject.get_elegiable_orfs(
max_orf_start=exonQstart,min_orf_end=exonQstop):
orfPhase = (exonQstart - orfObj.startPY) % 3
if orfPhase == dPhase:
tinyexonorf = orfObj
break
else:
# No tinyexonorf assigned!! Iin case a regex matched
# over a STOP-codon or the regex length is smaller
# then the smallest Orf, no Orf can be assigned
continue
# filter for donor & acceptor score
dScore = _score_splice_site(seqmatch[-9:],splicetype='donor')
aScore = _score_splice_site(seqmatch[0:11],splicetype='acceptor')
if dScore < kwargs['min_donor_pssm_score']:
continue
if aScore < kwargs['min_acceptor_pssm_score']:
continue
# scan Orf for splicesites
tinyexonorf.scan_orf_for_pssm_splice_sites(
splicetype="donor",
min_pssm_score=kwargs['min_donor_pssm_score'],
allow_non_canonical=kwargs['allow_non_canonical_donor'],
non_canonical_min_pssm_score=kwargs['non_canonical_min_donor_pssm_score'])
tinyexonorf.scan_orf_for_pssm_splice_sites(
splicetype="acceptor",
min_pssm_score=kwargs['min_acceptor_pssm_score'],
allow_non_canonical=kwargs['allow_non_canonical_acceptor'],
non_canonical_min_pssm_score=kwargs['non_canonical_min_acceptor_pssm_score'])
# get 1th intron donor object
intron1_aObj = None
for a in tinyexonorf._acceptor_sites:
if a.pos == exonQstart:
intron1_aObj = a
break
else:
# pseudo-acceptorsite as found be SFM regex
# is not a valid acceptor site of high enough score
# continue to next iteration of (hdr,seqmatch) pair
continue
# get 2th intron donor object
intron2_dObj = None
for d in tinyexonorf._donor_sites:
if d.pos == exonQstop:
intron2_dObj = d
break
else:
# pseudo-donorsite as found be SFM regex
# is not a valid acceptor site of high enough score
# continue to next iteration of (hdr,seqmatch) pair
continue
# check if introns are of elegiable lengths
if (intron1_aObj.pos-dObj.pos) > kwargs['max_intron_nt_length']:
continue
if (aObj.pos-intron2_dObj.pos) > kwargs['max_intron_nt_length']:
continue
####################################################
if True or verbose:
# if here, a candidate!!!
print (pacbporfD.orfQ.id,tinyexonorf.id,pacbporfA.orfQ.id),
print hdr, dScore, aScore
print seqmatch
####################################################
# append to found tinyexons
query_data = ( tinyexonorf, exonQstart, exonQstop )
sbjct_data = ( prjctOrf, posDsbjct, posAsbjct )
splicesite_data = ( dObj,intron1_aObj, intron2_dObj, aObj )
tinyexons.append( ( query_data, sbjct_data, splicesite_data ) )
# file cleanup
osRemove(fname)
# return - End Of Function - if no tinyexons are found
if not tinyexons:
return []
####################################
# select the **best** tinyexon
####################################
(query_data,sbjct_data,splicesite_data) = tinyexons[0]
orfQ,query_dna_start,query_dna_end = query_data
orfS,sbjct_dna_start,sbjct_dna_end = sbjct_data
(intron1_dObj,intron1_aObj,intron2_dObj,intron2_aObj) = splicesite_data
####################################################
if verbose:
print "tinyexon orf:", orfQ
print "tinyexon orf:", intron1_aObj
print "tinyexon orf:", intron2_dObj
####################################################
####################################
# make tinyexon PacbPORF
####################################
startQaa = orfQ.dnapos2aapos(query_dna_start) -1
startSaa = orfS.dnapos2aapos(sbjct_dna_start) -1
stopQaa = orfQ.dnapos2aapos(query_dna_end) +1
stopSaa = orfS.dnapos2aapos(sbjct_dna_end) +1
# check for directly leading stop codon on tinyexon
while startQaa <= orfQ.protein_startPY:
startQaa+=1
startSaa+=1
query_dna_start+=3
sbjct_dna_start+=3
while startSaa <= orfS.protein_startPY:
startQaa+=1
startSaa+=1
query_dna_start+=3
sbjct_dna_start+=3
# check for directly tailing stop codon on tinyexon
while stopQaa > orfQ.protein_endPY:
stopQaa-=1
stopSaa-=1
query_dna_end-=3
sbjct_dna_end-=3
while stopSaa > orfS.protein_endPY:
stopQaa-=1
stopSaa-=1
query_dna_end-=3
sbjct_dna_end-=3
# get sequences
qAAseq = orfQ.getaas(abs_pos_start=startQaa,abs_pos_end=stopQaa)
sAAseq = orfS.getaas(abs_pos_start=startSaa,abs_pos_end=stopSaa)
####################################################
if verbose or len(qAAseq) != len(sAAseq):
# if unequal lengths, error will be raised upon PacbP.__init__()
print orfQ, qAAseq, startQaa, stopQaa, (stopQaa-startQaa),
print (query_dna_start,query_dna_end)
print orfS, sAAseq, startSaa, stopSaa, (stopSaa-startSaa),
print (sbjct_dna_start,sbjct_dna_end)
print orfQ.inputgenomicsequence[query_dna_start-2:query_dna_end+2]
print orfS.inputgenomicsequence[sbjct_dna_start-2:sbjct_dna_end+2]
####################################################
# initialize extended tinyexon PacbPORF
from pacb import PacbP
pacbp = PacbP(input=( qAAseq, sAAseq, startQaa, startSaa ) )
pacbp.strip_unmatched_ends()
pacbporf = pacbp2pacbporf(pacbp,orfQ,orfS)
pacbporf.extend_pacbporf_after_stops()
pacbporf.source = 'ABGPprojectingTE'
####################################
# make introns
####################################
intron1 = IntronConnectingOrfs(
intron1_dObj, intron1_aObj, None,
donorOrf,pacbporf.orfQ )
intron2 = IntronConnectingOrfs(
intron2_dObj, intron2_aObj, None,
pacbporf.orfQ, accepOrf )
################################################################
# set some meta-data properties to the intron objects
################################################################
# add distance score to intron
intron1._distance = 0
intron2._distance = 0
# add Alignment Positional Periphery Score into objects
if queryorsbjct == "query":
succes = set_apps_intron_query(intron1,pacbporfD,pacbporf)
succes = set_apps_intron_query(intron2,pacbporf,pacbporfA)
else:
succes = set_apps_intron_sbjct(intron1,pacbporfD,pacbporf)
succes = set_apps_intron_sbjct(intron2,pacbporf,pacbporfA)
# set GFF fsource attribute for recognition of intron sources
intron1._gff['fsource'] = "ABGPprojectingTE"
intron2._gff['fsource'] = "ABGPprojectingTE"
# create _linked_to_xxx attributes
intron1._linked_to_pacbporfs = [ pacbporf ]
intron2._linked_to_pacbporfs = [ pacbporf ]
intron1._linked_to_introns = [ intron2 ]
intron2._linked_to_introns = [ intron1 ]
####################################################
if verbose:
print pacbporf
pacbporf.print_protein_and_dna()
print intron1
print intron2
if False:
# printing data when this function needs to be debugged:
print ""
print intron1
print intron2
print ""
print pacbporfD
pacbporfD.print_protein_and_dna()
print ""
print pacbporf
pacbporf.print_protein_and_dna()
print ""
print pacbporfA
pacbporfA.print_protein_and_dna()
import sys
sys.exit()
####################################################
# return introns and intermediate tinyexon PacbPORF
return [(intron1,intron2,pacbporf)]
def _merge_pacbporfs_by_two_tinyexons(pacbporfD,pacbporfA,
orfSetObject,queryorsbjct,verbose = False, **kwargs):
""" """
# edit **kwargs dictionary for some forced attributes
_update_kwargs(kwargs,KWARGS_PROJECTED_TINYEXON)
tinyexons = []
sposD = pacbporfD._get_original_alignment_pos_start()
eposD = pacbporfD._get_original_alignment_pos_end()
sposA = pacbporfA._get_original_alignment_pos_start()
eposA = pacbporfA._get_original_alignment_pos_end()
if queryorsbjct == "query":
donorOrf = pacbporfD.orfQ
accepOrf = pacbporfA.orfQ
prjctOrf = pacbporfD.orfS
dStart,dEnd = sposD.query_dna_start, eposD.query_dna_end
aStart,aEnd = sposA.query_dna_start, eposA.query_dna_end
elif queryorsbjct == "sbjct":
donorOrf = pacbporfD.orfS
accepOrf = pacbporfA.orfS
prjctOrf = pacbporfD.orfQ
dStart,dEnd = sposD.sbjct_dna_start, eposD.sbjct_dna_end
aStart,aEnd = sposA.sbjct_dna_start, eposA.sbjct_dna_end
else:
message = "'queryorsbjct' (%s), not 'query' or 'sbjct'" % queryorsbjct
raise InproperlyAppliedArgument, message
# get all potential combinations of two tinyexons
tinyexoncombis = merge_orfs_with_two_tinyexons(
donorOrf, accepOrf,
donorOrf._donor_sites,
accepOrf._acceptor_sites,
orfSetObject.orfs,
)
results = []
for dObj in donorOrf._donor_sites:
if queryorsbjct == "query":
(dPos,dPhase) = pacbporfD.dnaposition_query(dObj.pos,forced_return=True)
else:
(dPos,dPhase) = pacbporfD.dnaposition_sbjct(dObj.pos,forced_return=True)
try:
algDobj = pacbporfD._positions[dPos]
except IndexError:
# site out of range of PacbPORF -> break
break
# check if dObj is on pfD;
# introns of tinyexons can be projected outside of pfD/pfA area
if dObj.pos < dStart: continue
for aObj in accepOrf._acceptor_sites:
if queryorsbjct == "query":
(aPos,aPhase) = pacbporfA.dnaposition_query(aObj.pos,forced_return=True)
else:
(aPos,aPhase) = pacbporfA.dnaposition_sbjct(aObj.pos,forced_return=True)
try:
algAobj = pacbporfA._positions[aPos]
except IndexError:
# site out of range of PacbPORF -> break
break
# check if aObj is on pfA;
# introns of tinyexons can be projected outside of pfD/pfA area
if aObj.pos > aEnd: continue
if queryorsbjct == "query":
posDsbjct = algDobj.sbjct_dna_start + dPhase
posAsbjct = algAobj.sbjct_dna_start + aPhase
else:
posDsbjct = algDobj.query_dna_start + dPhase
posAsbjct = algAobj.query_dna_start + aPhase
distance = posAsbjct - posDsbjct
if distance >= (kwargs['max_tinyexon_nt_length']*2):
break
if distance < (kwargs['min_tinyexon_nt_length']*2):
continue
filtered_tinyexoncombis = _filter_tinyexoncombis(tinyexoncombis,
min_length = distance,
max_length = distance,
min_first_acceptor_pos = dObj.pos + kwargs['min_tinyexon_intron_nt_length'],
max_final_donor_pos = aObj.pos - kwargs['min_tinyexon_intron_nt_length'],
phase_final_donor = aObj.phase,
phase_first_acceptor= dObj.phase,
)
if not filtered_tinyexoncombis: continue
####################################################################
if verbose:
print distance, dObj, aObj, len(tinyexoncombis),
print len(filtered_tinyexoncombis)
####################################################################
for exon1,intron,exon2 in filtered_tinyexoncombis:
# make preceding intron
preceding_intron = IntronConnectingOrfs(
dObj,exon1.acceptor,
None,donorOrf,exon1.orf )
# make subsequent intron
subsequent_intron = IntronConnectingOrfs(
exon2.donor, aObj,
None,exon2.orf,accepOrf)
################################################################
if verbose:
print "\t", exon1, exon1.proteinsequence(),
print preceding_intron.phase, exon1.donor.phase,
print subsequent_intron.phase, preceding_intron.shared_aa,
print intron.shared_aa, subsequent_intron.shared_aa
print "\t", exon2, exon2.proteinsequence()
################################################################
# get prjctOrf sequence for comparison
correctionA = 0
if aObj.phase != 0:
# INCLUDE the final AA which is broken by the splicesite
correctionA=1
if queryorsbjct == "query":
startPos,_phase = pacbporfD.dnaposition_query(dObj.pos,forced_return=True)
stopPos,_phase = pacbporfA.dnaposition_query(aObj.pos,forced_return=True)
start = pacbporfD._positions[startPos].sbjct_pos
stop = pacbporfA._positions[stopPos].sbjct_pos + correctionA
else:
startPos,_phase = pacbporfD.dnaposition_sbjct(dObj.pos,forced_return=True)
stopPos,_phase = pacbporfA.dnaposition_sbjct(aObj.pos,forced_return=True)
start = pacbporfD._positions[startPos].query_pos
stop = pacbporfA._positions[stopPos].query_pos + correctionA
if stop <= start:
# tinyexon is so tiny that is does not have a single
# full aligned AA -> discard here
continue
# actually get the prjctOrf sequence
aaseq = prjctOrf.getaas(abs_pos_start=start,abs_pos_end=stop)
# initialize a PacbP for the combination of both tinyexons
# afterwards, check if the indentityscore is > 0.XX
from pacb import PacbP
seqparts = [ preceding_intron.shared_aa,
exon1.proteinsequence(),
intron.shared_aa,
exon2.proteinsequence(),
subsequent_intron.shared_aa ]
################################################################
if verbose or len("".join(seqparts)) != len(aaseq):
print pacbporfD
print exon1.orf, exon2.orf, prjctOrf
print pacbporfA
print seqparts
print aaseq, len(aaseq), len("".join(seqparts)), (start,stop)
print "'%s'" % queryorsbjct,
print "Q", (algDobj.query_pos, algAobj.query_pos),
print "S", (algDobj.sbjct_pos, algAobj.sbjct_pos)
print "distance:", distance, kwargs['max_tinyexon_nt_length'],
print (posDsbjct, posAsbjct),
print "Q-dna:", ( algDobj.query_dna_start, dPhase, algAobj.query_dna_start, aPhase ),
print "S-dna:", ( algDobj.sbjct_dna_start, dPhase, algAobj.sbjct_dna_start, aPhase )
################################################################
# ignore by continue when sequences not identical in length
if len("".join(seqparts)) != len(aaseq): continue
testpacbp = PacbP(input=( "".join(seqparts), aaseq, 0, 0) )
testpacbp.strip_unmatched_ends()
if not ( testpacbp.identityscore > 0.60 and\
(float(testpacbp.length) / len(aaseq)) > 0.70 ):
# not a very convincing alignment
continue
################################################################
if verbose:
print testpacbp
testpacbp.print_protein()
################################################################
# if here, succesfully mapped 2 tiny exons!!
# get all sequences/coordinates in place for
# pacbporf formation
orfQ1 = exon1.orf
orfS1 = prjctOrf
orfQ2 = exon2.orf
orfS2 = prjctOrf
seqQ1 = exon1.proteinsequence()
seqQ2 = exon2.proteinsequence()
coordQ1 = exon1.acceptor.pos / 3
coordS1 = start
coordQ2 = exon2.acceptor.pos / 3
coordS2 = start + len(seqparts[0]) + len(seqparts[1]) + len(seqparts[2])
seqS1 = aaseq[0:(len(seqparts[0])+len(seqparts[1]))]
seqS2 = aaseq[-(len(seqparts[3])+len(seqparts[4])):]
if len(seqparts[0]):
seqS1 = seqS1[1:]
coordS1 += 1
if len(seqparts[4]):
seqS2 = seqS2[:-1]
if queryorsbjct == "sbjct":
# swap query <-> sbjct
orfQ1,orfS1 = orfS1,orfQ1
orfQ2,orfS2 = orfS2,orfQ2
seqQ1,seqS1 = seqS1,seqQ1
seqQ2,seqS2 = seqS2,seqQ2
coordQ1,coordS1 = coordS1,coordQ1
coordQ2,coordS2 = coordS2,coordQ2
################################################################
if verbose:
print "tinypacbporf1:", seqQ1, seqQ2, coordQ1, coordQ2
print "tinypacbporf2:", seqS1, seqS2, coordS1, coordS2
################################################################
# make pacbporfs
pacbp1 = PacbP(input=( seqQ1, seqS1, coordQ1, coordS1) )
pacbp1.strip_unmatched_ends()
tinypacbporf1 = pacbp2pacbporf(pacbp1,orfQ1,orfS1)
tinypacbporf1.extend_pacbporf_after_stops()
pacbp2 = PacbP(input=( seqQ2, seqS2, coordQ2, coordS2) )
pacbp2.strip_unmatched_ends()
tinypacbporf2 = pacbp2pacbporf(pacbp2,orfQ2,orfS2)
tinypacbporf2.extend_pacbporf_after_stops()
################################################################
if verbose:
print tinypacbporf1
tinypacbporf1.print_protein_and_dna()
print tinypacbporf2
tinypacbporf2.print_protein_and_dna()
################################################################
################################################################
# set some meta-data properties to the intron objects
################################################################
# add distance score to intron
preceding_intron._distance = 0
intron._distance = 0
subsequent_intron._distance = 0
# add Alignment Positional Periphery Score into objects
if queryorsbjct == "query":
succes = set_apps_intron_query(preceding_intron,pacbporfD,tinypacbporf1)
succes = set_apps_intron_query(intron,tinypacbporf1,tinypacbporf2)
succes = set_apps_intron_query(subsequent_intron,tinypacbporf2,pacbporfA)
else:
succes = set_apps_intron_sbjct(preceding_intron,pacbporfD,tinypacbporf1)
succes = set_apps_intron_sbjct(intron,tinypacbporf1,tinypacbporf2)
succes = set_apps_intron_sbjct(subsequent_intron,tinypacbporf2,pacbporfA)
# set GFF fsource attribute for recognition of intron sources
preceding_intron._gff['fsource'] = "ABGPprojectingTE"
intron._gff['fsource'] = "ABGPprojectingTE"
subsequent_intron._gff['fsource'] = "ABGPprojectingTE"
# create _linked_to_xxx attributes
preceding_intron._linked_to_pacbporfs = [ tinypacbporf1, tinypacbporf2 ]
intron._linked_to_pacbporfs = [ tinypacbporf1, tinypacbporf2 ]
subsequent_intron._linked_to_pacbporfs = [ tinypacbporf1, tinypacbporf2 ]
preceding_intron._linked_to_introns = [ intron,subsequent_intron ]
intron._linked_to_introns = [ preceding_intron,subsequent_intron ]
subsequent_intron._linked_to_introns = [ intron,preceding_intron ]
################################################################
# append to results
################################################################
results.append( (
preceding_intron,
intron,
subsequent_intron,
tinypacbporf1,
tinypacbporf2,
) )
# return 3 introns and 2 intermediate tinyexon PacbPORFs (per row)
return results
def _merge_pacbporfs_by_two_tinyexons(pacbporfD,
pacbporfA,
orfSetObject,
queryorsbjct,
verbose=False,
**kwargs):
""" """
# edit **kwargs dictionary for some forced attributes
_update_kwargs(kwargs, KWARGS_PROJECTED_TINYEXON)
tinyexons = []
sposD = pacbporfD._get_original_alignment_pos_start()
eposD = pacbporfD._get_original_alignment_pos_end()
sposA = pacbporfA._get_original_alignment_pos_start()
eposA = pacbporfA._get_original_alignment_pos_end()
if queryorsbjct == "query":
donorOrf = pacbporfD.orfQ
accepOrf = pacbporfA.orfQ
prjctOrf = pacbporfD.orfS
dStart, dEnd = sposD.query_dna_start, eposD.query_dna_end
aStart, aEnd = sposA.query_dna_start, eposA.query_dna_end
elif queryorsbjct == "sbjct":
donorOrf = pacbporfD.orfS
accepOrf = pacbporfA.orfS
prjctOrf = pacbporfD.orfQ
dStart, dEnd = sposD.sbjct_dna_start, eposD.sbjct_dna_end
aStart, aEnd = sposA.sbjct_dna_start, eposA.sbjct_dna_end
else:
message = "'queryorsbjct' (%s), not 'query' or 'sbjct'" % queryorsbjct
raise InproperlyAppliedArgument, message
# get all potential combinations of two tinyexons
tinyexoncombis = merge_orfs_with_two_tinyexons(
donorOrf,
accepOrf,
donorOrf._donor_sites,
accepOrf._acceptor_sites,
orfSetObject.orfs,
)
results = []
for dObj in donorOrf._donor_sites:
if queryorsbjct == "query":
(dPos, dPhase) = pacbporfD.dnaposition_query(dObj.pos,
forced_return=True)
else:
(dPos, dPhase) = pacbporfD.dnaposition_sbjct(dObj.pos,
forced_return=True)
try:
algDobj = pacbporfD._positions[dPos]
except IndexError:
# site out of range of PacbPORF -> break
break
# check if dObj is on pfD;
# introns of tinyexons can be projected outside of pfD/pfA area
if dObj.pos < dStart: continue
for aObj in accepOrf._acceptor_sites:
if queryorsbjct == "query":
(aPos,
aPhase) = pacbporfA.dnaposition_query(aObj.pos,
forced_return=True)
else:
(aPos,
aPhase) = pacbporfA.dnaposition_sbjct(aObj.pos,
forced_return=True)
try:
algAobj = pacbporfA._positions[aPos]
except IndexError:
# site out of range of PacbPORF -> break
break
# check if aObj is on pfA;
# introns of tinyexons can be projected outside of pfD/pfA area
if aObj.pos > aEnd: continue
if queryorsbjct == "query":
posDsbjct = algDobj.sbjct_dna_start + dPhase
posAsbjct = algAobj.sbjct_dna_start + aPhase
else:
posDsbjct = algDobj.query_dna_start + dPhase
posAsbjct = algAobj.query_dna_start + aPhase
distance = posAsbjct - posDsbjct
if distance >= (kwargs['max_tinyexon_nt_length'] * 2):
break
if distance < (kwargs['min_tinyexon_nt_length'] * 2):
continue
filtered_tinyexoncombis = _filter_tinyexoncombis(
tinyexoncombis,
min_length=distance,
max_length=distance,
min_first_acceptor_pos=dObj.pos +
kwargs['min_tinyexon_intron_nt_length'],
max_final_donor_pos=aObj.pos -
kwargs['min_tinyexon_intron_nt_length'],
phase_final_donor=aObj.phase,
phase_first_acceptor=dObj.phase,
)
if not filtered_tinyexoncombis: continue
####################################################################
if verbose:
print distance, dObj, aObj, len(tinyexoncombis),
print len(filtered_tinyexoncombis)
####################################################################
for exon1, intron, exon2 in filtered_tinyexoncombis:
# make preceding intron
preceding_intron = IntronConnectingOrfs(
dObj, exon1.acceptor, None, donorOrf, exon1.orf)
# make subsequent intron
subsequent_intron = IntronConnectingOrfs(
exon2.donor, aObj, None, exon2.orf, accepOrf)
################################################################
if verbose:
print "\t", exon1, exon1.proteinsequence(),
print preceding_intron.phase, exon1.donor.phase,
print subsequent_intron.phase, preceding_intron.shared_aa,
print intron.shared_aa, subsequent_intron.shared_aa
print "\t", exon2, exon2.proteinsequence()
################################################################
# get prjctOrf sequence for comparison
correctionA = 0
if aObj.phase != 0:
# INCLUDE the final AA which is broken by the splicesite
correctionA = 1
if queryorsbjct == "query":
startPos, _phase = pacbporfD.dnaposition_query(
dObj.pos, forced_return=True)
stopPos, _phase = pacbporfA.dnaposition_query(
aObj.pos, forced_return=True)
start = pacbporfD._positions[startPos].sbjct_pos
stop = pacbporfA._positions[stopPos].sbjct_pos + correctionA
else:
startPos, _phase = pacbporfD.dnaposition_sbjct(
dObj.pos, forced_return=True)
stopPos, _phase = pacbporfA.dnaposition_sbjct(
aObj.pos, forced_return=True)
start = pacbporfD._positions[startPos].query_pos
stop = pacbporfA._positions[stopPos].query_pos + correctionA
if stop <= start:
# tinyexon is so tiny that is does not have a single
# full aligned AA -> discard here
continue
# actually get the prjctOrf sequence
aaseq = prjctOrf.getaas(abs_pos_start=start, abs_pos_end=stop)
# initialize a PacbP for the combination of both tinyexons
# afterwards, check if the indentityscore is > 0.XX
from pacb import PacbP
seqparts = [
preceding_intron.shared_aa,
exon1.proteinsequence(), intron.shared_aa,
exon2.proteinsequence(), subsequent_intron.shared_aa
]
################################################################
if verbose or len("".join(seqparts)) != len(aaseq):
print pacbporfD
print exon1.orf, exon2.orf, prjctOrf
print pacbporfA
print seqparts
print aaseq, len(aaseq), len("".join(seqparts)), (start,
stop)
print "'%s'" % queryorsbjct,
print "Q", (algDobj.query_pos, algAobj.query_pos),
print "S", (algDobj.sbjct_pos, algAobj.sbjct_pos)
print "distance:", distance, kwargs[
'max_tinyexon_nt_length'],
print(posDsbjct, posAsbjct),
print "Q-dna:", (algDobj.query_dna_start, dPhase,
algAobj.query_dna_start, aPhase),
print "S-dna:", (algDobj.sbjct_dna_start, dPhase,
algAobj.sbjct_dna_start, aPhase)
################################################################
# ignore by continue when sequences not identical in length
if len("".join(seqparts)) != len(aaseq): continue
testpacbp = PacbP(input=("".join(seqparts), aaseq, 0, 0))
testpacbp.strip_unmatched_ends()
if not ( testpacbp.identityscore > 0.60 and\
(float(testpacbp.length) / len(aaseq)) > 0.70 ):
# not a very convincing alignment
continue
################################################################
if verbose:
print testpacbp
testpacbp.print_protein()
################################################################
# if here, succesfully mapped 2 tiny exons!!
# get all sequences/coordinates in place for
# pacbporf formation
orfQ1 = exon1.orf
orfS1 = prjctOrf
orfQ2 = exon2.orf
orfS2 = prjctOrf
seqQ1 = exon1.proteinsequence()
seqQ2 = exon2.proteinsequence()
coordQ1 = exon1.acceptor.pos / 3
coordS1 = start
coordQ2 = exon2.acceptor.pos / 3
coordS2 = start + len(seqparts[0]) + len(seqparts[1]) + len(
seqparts[2])
seqS1 = aaseq[0:(len(seqparts[0]) + len(seqparts[1]))]
seqS2 = aaseq[-(len(seqparts[3]) + len(seqparts[4])):]
if len(seqparts[0]):
seqS1 = seqS1[1:]
coordS1 += 1
if len(seqparts[4]):
seqS2 = seqS2[:-1]
if queryorsbjct == "sbjct":
# swap query <-> sbjct
orfQ1, orfS1 = orfS1, orfQ1
orfQ2, orfS2 = orfS2, orfQ2
seqQ1, seqS1 = seqS1, seqQ1
seqQ2, seqS2 = seqS2, seqQ2
coordQ1, coordS1 = coordS1, coordQ1
coordQ2, coordS2 = coordS2, coordQ2
################################################################
if verbose:
print "tinypacbporf1:", seqQ1, seqQ2, coordQ1, coordQ2
print "tinypacbporf2:", seqS1, seqS2, coordS1, coordS2
################################################################
# make pacbporfs
pacbp1 = PacbP(input=(seqQ1, seqS1, coordQ1, coordS1))
pacbp1.strip_unmatched_ends()
tinypacbporf1 = pacbp2pacbporf(pacbp1, orfQ1, orfS1)
tinypacbporf1.extend_pacbporf_after_stops()
pacbp2 = PacbP(input=(seqQ2, seqS2, coordQ2, coordS2))
pacbp2.strip_unmatched_ends()
tinypacbporf2 = pacbp2pacbporf(pacbp2, orfQ2, orfS2)
tinypacbporf2.extend_pacbporf_after_stops()
################################################################
if verbose:
print tinypacbporf1
tinypacbporf1.print_protein_and_dna()
print tinypacbporf2
tinypacbporf2.print_protein_and_dna()
################################################################
################################################################
# set some meta-data properties to the intron objects
################################################################
# add distance score to intron
preceding_intron._distance = 0
intron._distance = 0
subsequent_intron._distance = 0
# add Alignment Positional Periphery Score into objects
if queryorsbjct == "query":
succes = set_apps_intron_query(preceding_intron, pacbporfD,
tinypacbporf1)
succes = set_apps_intron_query(intron, tinypacbporf1,
tinypacbporf2)
succes = set_apps_intron_query(subsequent_intron,
tinypacbporf2, pacbporfA)
else:
succes = set_apps_intron_sbjct(preceding_intron, pacbporfD,
tinypacbporf1)
succes = set_apps_intron_sbjct(intron, tinypacbporf1,
tinypacbporf2)
succes = set_apps_intron_sbjct(subsequent_intron,
tinypacbporf2, pacbporfA)
# set GFF fsource attribute for recognition of intron sources
preceding_intron._gff['fsource'] = "ABGPprojectingTE"
intron._gff['fsource'] = "ABGPprojectingTE"
subsequent_intron._gff['fsource'] = "ABGPprojectingTE"
# create _linked_to_xxx attributes
preceding_intron._linked_to_pacbporfs = [
tinypacbporf1, tinypacbporf2
]
intron._linked_to_pacbporfs = [tinypacbporf1, tinypacbporf2]
subsequent_intron._linked_to_pacbporfs = [
tinypacbporf1, tinypacbporf2
]
preceding_intron._linked_to_introns = [
intron, subsequent_intron
]
intron._linked_to_introns = [
preceding_intron, subsequent_intron
]
subsequent_intron._linked_to_introns = [
intron, preceding_intron
]
################################################################
# append to results
################################################################
results.append((
preceding_intron,
intron,
subsequent_intron,
tinypacbporf1,
tinypacbporf2,
))
# return 3 introns and 2 intermediate tinyexon PacbPORFs (per row)
return results
def _merge_pacbporfs_by_tinyexon_and_two_introns(pacbporfD,
pacbporfA,
orfSetObject,
queryorsbjct,
verbose=False,
**kwargs):
"""
Merge 2 PacbPORF objects by introns
@attention: see pacb.connecting.merge_orfs_with_intron for **kwargs)
@type pacbporfD: PacbPORF object
@param pacbporfD: PacbPORF object that has to deliver PSSM donor objects
@type pacbporfA: PacbPORF object
@param pacbporfA: PacbPORF object that has to deliver PSSM acceptor objects
@type orfSetObject: object with elegiable Orfs
@param orfSetObject: object with elegiable Orfs
@type queryorsbjct: string
@param queryorsbjct: literal string 'query' or 'sbjct'
@type verbose: Boolean
@param verbose: print debugging info to STDOUT when True
@rtype: list
@return: list with ( intron, ExonOnOrf, intron ) on the query sequence
"""
# input validation
IsPacbPORF(pacbporfD)
IsPacbPORF(pacbporfA)
# edit **kwargs dictionary for some forced attributes
_update_kwargs(kwargs, KWARGS_PROJECTED_TINYEXON)
MAX_TINYEXON_NT_LENGTH = 33
MIN_TINYEXON_NT_LENGTH = 6
tinyexons = []
if queryorsbjct == "query":
donorOrf = pacbporfD.orfQ
accepOrf = pacbporfA.orfQ
prjctOrf = pacbporfD.orfS
alignedDonorRange = pacbporfD.alignment_dna_range_query()
alignedAccepRange = pacbporfA.alignment_dna_range_query()
elif queryorsbjct == "sbjct":
donorOrf = pacbporfD.orfS
accepOrf = pacbporfA.orfS
prjctOrf = pacbporfD.orfQ
alignedDonorRange = pacbporfD.alignment_dna_range_sbjct()
alignedAccepRange = pacbporfA.alignment_dna_range_sbjct()
else:
message = "'queryorsbjct' (%s), not 'query' or 'sbjct'" % queryorsbjct
raise InproperlyAppliedArgument, message
for dObj in donorOrf._donor_sites:
# do not make a projection OVER the aligned area
if dObj.pos < min(alignedDonorRange): continue
if queryorsbjct == "query":
(dPos, dPhase) = pacbporfD.dnaposition_query(dObj.pos,
forced_return=True)
else:
(dPos, dPhase) = pacbporfD.dnaposition_sbjct(dObj.pos,
forced_return=True)
try:
algDobj = pacbporfD._positions[dPos]
except IndexError:
# site out of range of PacbPORF -> break
break
for aObj in accepOrf._acceptor_sites:
# do not make a projection OVER the aligned area
if aObj.pos > max(alignedAccepRange): continue
if queryorsbjct == "query":
(aPos,
aPhase) = pacbporfA.dnaposition_query(aObj.pos,
forced_return=True)
else:
(aPos,
aPhase) = pacbporfA.dnaposition_sbjct(aObj.pos,
forced_return=True)
try:
algAobj = pacbporfA._positions[aPos]
except IndexError:
# site out of range of PacbPORF -> break
break
if queryorsbjct == "query":
posDsbjct = algDobj.sbjct_dna_start + dPhase
posAsbjct = algAobj.sbjct_dna_start + aPhase
else:
posDsbjct = algDobj.query_dna_start + dPhase
posAsbjct = algAobj.query_dna_start + aPhase
distance = posAsbjct - posDsbjct
if distance >= MAX_TINYEXON_NT_LENGTH:
break
if distance < MIN_TINYEXON_NT_LENGTH:
continue
####################################################
# generate a ScanForMatches pattern file
####################################################
# example pattern: 6...6 AG NNGNNANNANNGN[2,0,0] GT 3...3
query = list(prjctOrf.inputgenomicsequence[posDsbjct:posAsbjct])
# mask all non-phase0 nucleotides to N residues;
# this represents the regularexpression for a specific
# peptide sequence
firstphasepositions = range(3 - dPhase % 3, len(query), 3)
for pos in range(0, len(query)):
if pos not in firstphasepositions:
query[pos] = "N"
# calculate a ~50% mismatch number
mismatches = max([0, (len(query) - query.count("N")) / 2])
# write the pattern to string and subsequently to file
# example pattern: 6...6 AG NNGNNANNANNGN[2,0,0] GT 3...3
if kwargs['allow_non_canonical_donor']:
sfmpat = "%s...%s AG %s[%s,0,0] G (T | C) %s...%s" % (
AUSO, AUSO, "".join(query), mismatches, DDSO, DDSO)
else:
sfmpat = "%s...%s AG %s[%s,0,0] GT %s...%s" % (
AUSO, AUSO, "".join(query), mismatches, DDSO, DDSO)
####################################################
if verbose:
print(pacbporfD.orfQ.id, pacbporfA.orfQ.id),
print distance, dObj, aObj
print sfmpat
####################################################
fname = "sfmpat_tinyexon_%s_%s_%s_%s" % (
donorOrf.id,
accepOrf.id,
posDsbjct,
posAsbjct,
)
fh = open(fname, 'w')
fh.write(sfmpat + "\n")
fh.close()
####################################################
# run ScanForMatches
####################################################
command = """echo ">myseq\n%s" | %s %s | tr "[,]" "\t\t#" | """ +\
"""tr -d "\n " | sed "s/>/\\n>/g" | tr "#" "\t" | """ +\
"""awk -F'\t' '{ if (NF==4 && $2>%s && $3<%s) """ +\
"""{ print $1"["$2","$3"]\\n"$4 } }' """
command = command % (donorOrf.inputgenomicsequence, EXECUTABLE_SFM,
fname, dObj.pos +
(kwargs['min_intron_nt_length'] - 3),
aObj.pos -
(kwargs['min_intron_nt_length'] - 3))
co = osPopen(command)
matches = parseFasta(co.readlines())
co.close()
# filter matches for:
# (1) correct donor & acceptor phase
# (2) high enough donor & acceptor site scores
for hdr, seqmatch in matches.iteritems():
startQ, stopQ = [
int(item) for item in hdr.split(":")[1][1:-1].split(",")
]
exonQstart = startQ + AUSO + 2 - 1
exonQstop = stopQ - DDSO - 2
####################################
# get Orf object of tinyexon
####################################
tinyexonorf = None
# select the Orf on which the tinyexon is located
for orfObj in orfSetObject.get_eligible_orfs(
max_orf_start=exonQstart, min_orf_end=exonQstop):
orfPhase = (exonQstart - orfObj.startPY) % 3
if orfPhase == dPhase:
tinyexonorf = orfObj
break
else:
# No tinyexonorf assigned!! Iin case a regex matched
# over a STOP-codon or the regex length is smaller
# then the smallest Orf, no Orf can be assigned
continue
# filter for donor & acceptor score
dScore = _score_splice_site(seqmatch[-9:], splicetype='donor')
aScore = _score_splice_site(seqmatch[0:11],
splicetype='acceptor')
if dScore < kwargs['min_donor_pssm_score']:
continue
if aScore < kwargs['min_acceptor_pssm_score']:
continue
# scan Orf for splicesites
tinyexonorf.scan_orf_for_pssm_splice_sites(
splicetype="donor",
min_pssm_score=kwargs['min_donor_pssm_score'],
allow_non_canonical=kwargs['allow_non_canonical_donor'],
non_canonical_min_pssm_score=kwargs[
'non_canonical_min_donor_pssm_score'])
tinyexonorf.scan_orf_for_pssm_splice_sites(
splicetype="acceptor",
min_pssm_score=kwargs['min_acceptor_pssm_score'],
allow_non_canonical=kwargs['allow_non_canonical_acceptor'],
non_canonical_min_pssm_score=kwargs[
'non_canonical_min_acceptor_pssm_score'])
# get 1th intron donor object
intron1_aObj = None
for a in tinyexonorf._acceptor_sites:
if a.pos == exonQstart:
intron1_aObj = a
break
else:
# pseudo-acceptorsite as found be SFM regex
# is not a valid acceptor site of high enough score
# continue to next iteration of (hdr,seqmatch) pair
continue
# get 2th intron donor object
intron2_dObj = None
for d in tinyexonorf._donor_sites:
if d.pos == exonQstop:
intron2_dObj = d
break
else:
# pseudo-donorsite as found be SFM regex
# is not a valid acceptor site of high enough score
# continue to next iteration of (hdr,seqmatch) pair
continue
# check if introns are of elegiable lengths
if (intron1_aObj.pos -
dObj.pos) > kwargs['max_intron_nt_length']:
continue
if (aObj.pos -
intron2_dObj.pos) > kwargs['max_intron_nt_length']:
continue
####################################################
if True or verbose:
# if here, a candidate!!!
print(pacbporfD.orfQ.id, tinyexonorf.id,
pacbporfA.orfQ.id),
print hdr, dScore, aScore
print seqmatch
####################################################
# append to found tinyexons
query_data = (tinyexonorf, exonQstart, exonQstop)
sbjct_data = (prjctOrf, posDsbjct, posAsbjct)
splicesite_data = (dObj, intron1_aObj, intron2_dObj, aObj)
tinyexons.append((query_data, sbjct_data, splicesite_data))
# file cleanup
osRemove(fname)
# return - End Of Function - if no tinyexons are found
if not tinyexons:
return []
####################################
# select the **best** tinyexon
####################################
(query_data, sbjct_data, splicesite_data) = tinyexons[0]
orfQ, query_dna_start, query_dna_end = query_data
orfS, sbjct_dna_start, sbjct_dna_end = sbjct_data
(intron1_dObj, intron1_aObj, intron2_dObj, intron2_aObj) = splicesite_data
####################################################
if verbose:
print "tinyexon orf:", orfQ
print "tinyexon orf:", intron1_aObj
print "tinyexon orf:", intron2_dObj
####################################################
####################################
# make tinyexon PacbPORF
####################################
startQaa = orfQ.dnapos2aapos(query_dna_start) - 1
startSaa = orfS.dnapos2aapos(sbjct_dna_start) - 1
stopQaa = orfQ.dnapos2aapos(query_dna_end) + 1
stopSaa = orfS.dnapos2aapos(sbjct_dna_end) + 1
# check for directly leading stop codon on tinyexon
while startQaa <= orfQ.protein_startPY:
startQaa += 1
startSaa += 1
query_dna_start += 3
sbjct_dna_start += 3
while startSaa <= orfS.protein_startPY:
startQaa += 1
startSaa += 1
query_dna_start += 3
sbjct_dna_start += 3
# check for directly tailing stop codon on tinyexon
while stopQaa > orfQ.protein_endPY:
stopQaa -= 1
stopSaa -= 1
query_dna_end -= 3
sbjct_dna_end -= 3
while stopSaa > orfS.protein_endPY:
stopQaa -= 1
stopSaa -= 1
query_dna_end -= 3
sbjct_dna_end -= 3
# get sequences
qAAseq = orfQ.getaas(abs_pos_start=startQaa, abs_pos_end=stopQaa)
sAAseq = orfS.getaas(abs_pos_start=startSaa, abs_pos_end=stopSaa)
####################################################
if verbose or len(qAAseq) != len(sAAseq):
# if unequal lengths, error will be raised upon PacbP.__init__()
print orfQ, qAAseq, startQaa, stopQaa, (stopQaa - startQaa),
print(query_dna_start, query_dna_end)
print orfS, sAAseq, startSaa, stopSaa, (stopSaa - startSaa),
print(sbjct_dna_start, sbjct_dna_end)
print orfQ.inputgenomicsequence[query_dna_start - 2:query_dna_end + 2]
print orfS.inputgenomicsequence[sbjct_dna_start - 2:sbjct_dna_end + 2]
####################################################
# initialize extended tinyexon PacbPORF
from pacb import PacbP
pacbp = PacbP(input=(qAAseq, sAAseq, startQaa, startSaa))
pacbp.strip_unmatched_ends()
pacbporf = pacbp2pacbporf(pacbp, orfQ, orfS)
pacbporf.extend_pacbporf_after_stops()
pacbporf.source = 'ABGPprojectingTE'
####################################
# make introns
####################################
intron1 = IntronConnectingOrfs(intron1_dObj, intron1_aObj, None, donorOrf,
pacbporf.orfQ)
intron2 = IntronConnectingOrfs(intron2_dObj, intron2_aObj, None,
pacbporf.orfQ, accepOrf)
################################################################
# set some meta-data properties to the intron objects
################################################################
# add distance score to intron
intron1._distance = 0
intron2._distance = 0
# add Alignment Positional Periphery Score into objects
if queryorsbjct == "query":
succes = set_apps_intron_query(intron1, pacbporfD, pacbporf)
succes = set_apps_intron_query(intron2, pacbporf, pacbporfA)
else:
succes = set_apps_intron_sbjct(intron1, pacbporfD, pacbporf)
succes = set_apps_intron_sbjct(intron2, pacbporf, pacbporfA)
# set GFF fsource attribute for recognition of intron sources
intron1._gff['fsource'] = "ABGPprojectingTE"
intron2._gff['fsource'] = "ABGPprojectingTE"
# create _linked_to_xxx attributes
intron1._linked_to_pacbporfs = [pacbporf]
intron2._linked_to_pacbporfs = [pacbporf]
intron1._linked_to_introns = [intron2]
intron2._linked_to_introns = [intron1]
####################################################
if verbose:
print pacbporf
pacbporf.print_protein_and_dna()
print intron1
print intron2
if False:
# printing data when this function needs to be debugged:
print ""
print intron1
print intron2
print ""
print pacbporfD
pacbporfD.print_protein_and_dna()
print ""
print pacbporf
pacbporf.print_protein_and_dna()
print ""
print pacbporfA
pacbporfA.print_protein_and_dna()
import sys
sys.exit()
####################################################
# return introns and intermediate tinyexon PacbPORF
return [(intron1, intron2, pacbporf)]
def merge_orfs_with_two_tinyexons(preceding_orf,
subsequent_orf,
preceding_donor_sites=[],
subsequent_acceptor_sites=[],
orflist=[],
**kwargs):
"""
Bridge two `neighbouring` Orfs by TWO tinyexon by applying preceding donors and subsequent acceptors
@type preceding_orf: Orf object
@param preceding_orf: Orf object that contains preceding_donor_site(s)
@type subsequent_orf: Orf object
@param subsequent_orf: Orf object that contains subsequent_acceptor_site(s)
@type preceding_donor_sites: list
@param preceding_donor_sites: list with SpliceDonorGT and/or SpliceDonor objects
@type subsequent_acceptor_sites: list
@param subsequent_acceptor_sites: list with SpliceAcceptorAG and/or SpliceAcceptor objects
@type orflist: list
@param orflist: list with Orf objects
@attention: see get_potential_tiny_exons_on_orf for additional **kwargs
@rtype: list
@return: list of tuples ( preceding_intron, tinyexon1, central_intron, tinyexon2, subsequent_intron )
"""
if not preceding_donor_sites:
return []
if not subsequent_acceptor_sites:
return []
if not orflist:
return []
# edit **kwargs dictionary for some forced attributes
_update_kwargs(kwargs, KWARGS_PROJECTED_TINYEXON)
# return list with (intron,tinyexon,intron) tuples
returntinyexons = []
tinyexoncollection = []
tinyexoncombis = []
min_preceding_donor_sites_pos = min([d.pos for d in preceding_donor_sites])
max_subsequent_acceptor_sites_pos = max(
[a.pos for a in subsequent_acceptor_sites])
for orfX in orflist:
# check if orf is correctly positions towards the splice sites' extremes
min_pos = min_preceding_donor_sites_pos + kwargs[
'min_tinyexon_intron_nt_length']
max_pos = max_subsequent_acceptor_sites_pos - kwargs[
'min_tinyexon_intron_nt_length']
# if so, do not check this Orf
if orfX.endPY <= min_pos: continue
if orfX.startPY >= max_pos: continue
# extend the tinyexoncollection
tinyexoncollection.extend(
get_potential_tiny_exons_on_orf(orfX, **kwargs))
# make tinyexoncollection ordered on start pos
tinyexoncollection = _order_intron_list(tinyexoncollection,
order_by='donor_pos')
# donor_pos makes REVERSE ordering; restore this by reversing
tinyexoncollection.reverse()
# make 2-elemented tuples of tinyexons which can co-occur together
for tinyexon1 in tinyexoncollection:
for pos in range(len(tinyexoncollection) - 1, -1, -1):
tinyexon2 = tinyexoncollection[pos]
if tinyexon2.donor.pos < tinyexon1.donor.pos: break
intron_length = tinyexon2.acceptor.pos - tinyexon1.donor.pos
if intron_length < kwargs['min_tinyexon_intron_nt_length']:
continue
if intron_length > kwargs['max_tinyexon_intron_nt_length']:
continue
if tinyexon1.donor.phase != tinyexon2.acceptor.phase: continue
# if here, elegiable combi!
intron = IntronConnectingOrfs(
tinyexon1.donor, tinyexon2.acceptor,
get_shared_nucleotides_at_splicesite(subsequent_orf,
preceding_orf,
tinyexon2.acceptor,
tinyexon1.donor),
preceding_orf, subsequent_orf)
totlen = tinyexon1.length + tinyexon2.length
combi = (totlen, tinyexon1, intron, tinyexon2)
tinyexoncombis.append(combi)
# return an ordered list based on length
tinyexoncombis.sort()
return [(exon1, intron, exon2)
for l, exon1, intron, exon2 in tinyexoncombis]
def merge_orfs_with_intron(
orfD,
orfA,
max_intron_nt_length=MAX_INTRON_NT_LENGTH,
min_intron_nt_length=MIN_INTRON_NT_LENGTH,
min_donor_pssm_score=MIN_DONOR_PSSM_SCORE,
min_acceptor_pssm_score=MIN_ACCEPTOR_PSSM_SCORE,
allow_non_canonical_donor=ALLOW_NON_CANONICAL_DONOR,
allow_non_canonical_acceptor=ALLOW_NON_CANONICAL_ACCEPTOR,
non_canonical_min_donor_pssm_score=NON_CANONICAL_MIN_DONOR_PSSM_SCORE,
non_canonical_min_acceptor_pssm_score=NON_CANONICAL_MIN_ACCEPTOR_PSSM_SCORE,
min_donor_pos=None,
max_donor_pos=None,
min_acceptor_pos=None,
max_acceptor_pos=None,
order_by='length',
**kwargs):
"""
Merge 2 Orf objects by introns
@attention: **kwargs can contain other (here) unnecessarily arguments
@type orfD: Orf object
@param orfD: Orf object that has to deliver a PSSM donor object
@type orfA: Orf object
@param orfA: Orf object that has to deliver a PSSM acceptor object
@type max_intron_nt_length: integer
@param max_intron_nt_length: maximal length (nt) of the intron
@type min_intron_nt_length: integer
@param min_intron_nt_length: minimal length (nt) of the intron
@type min_donor_pssm_score: float
@param min_donor_pssm_score: minimal pssm score of donor splice site
@type min_acceptor_pssm_score: float
@param min_acceptor_pssm_score: minimal pssm score of acceptor splice site
@type allow_non_canonical_donor: Boolean
@param allow_non_canonical_donor: search for non-canonical donor sites too
@type allow_non_canonical_acceptor: Boolean
@param allow_non_canonical_acceptor: search for non-canonical acceptor splice sites too
@type non_canonical_min_donor_pssm_score: float
@param non_canonical_min_donor_pssm_score: minimal pssm score of non-canonical donor
@type non_canonical_min_acceptor_pssm_score: float
@param non_canonical_min_acceptor_pssm_score: minimal pssm score of non-canonical acceptor
@rtype: list
@return: list with introns
"""
# input validation
IsOrf(orfD)
IsOrf(orfA)
# scan for splice sites (if not already done -> is checked in function)
orfD.scan_orf_for_pssm_splice_sites(
splicetype="donor",
min_pssm_score=min_donor_pssm_score,
allow_non_canonical=allow_non_canonical_donor,
non_canonical_min_pssm_score=non_canonical_min_donor_pssm_score)
orfA.scan_orf_for_pssm_splice_sites(
splicetype="acceptor",
min_pssm_score=min_acceptor_pssm_score,
allow_non_canonical=allow_non_canonical_acceptor,
non_canonical_min_pssm_score=non_canonical_min_acceptor_pssm_score)
# return list with introns
introns = []
# most quickest scan possible: are there donors & acceptors?
if orfD._donor_sites == [] or orfA._acceptor_sites == []:
# no introns possible because splice sites are missing
return introns
# very quick scan: are exons not to far from each other?
if max_intron_nt_length and\
(orfA._acceptor_sites[0].pos - orfD._donor_sites[0].pos) > max_intron_nt_length:
# no introns possible that can bridge this gap
return introns
for donor in orfD._donor_sites:
if not allow_non_canonical_donor and not donor.is_canonical():
continue
elif donor.is_canonical() and donor.pssm_score < min_donor_pssm_score:
continue
elif not donor.is_canonical(
) and donor.pssm_score < non_canonical_min_donor_pssm_score:
continue
elif (min_donor_pos
or min_donor_pos == 0) and donor.pos < min_donor_pos:
continue
elif (max_donor_pos
or max_donor_pos == 0) and donor.pos > max_donor_pos:
continue
else:
# donor site accepted
pass
for acceptor in orfA._acceptor_sites:
if not allow_non_canonical_acceptor and not acceptor.is_canonical(
):
continue
elif acceptor.is_canonical(
) and acceptor.pssm_score < min_acceptor_pssm_score:
continue
elif not acceptor.is_canonical(
) and acceptor.pssm_score < non_canonical_min_acceptor_pssm_score:
continue
elif (min_acceptor_pos or min_acceptor_pos
== 0) and acceptor.pos < min_acceptor_pos:
continue
elif (max_acceptor_pos or max_acceptor_pos
== 0) and acceptor.pos > max_acceptor_pos:
continue
else:
# acceptor site accepted
pass
# generate intron length and phase variable
intron_length = acceptor.pos - donor.pos
intron_phase = intron_length % 3
# check phase compatibilty (1) of splice sites
if donor.phase != acceptor.phase: continue
# check phase compatibilty (2) of splice sites
if (intron_phase + orfD.frame) % 3 != orfA.frame % 3: continue
# check if intron length is in between the boundaries
if max_intron_nt_length and intron_length > max_intron_nt_length:
continue
if min_intron_nt_length and intron_length < min_intron_nt_length:
continue
# okay, if we reach this point, we have a valid intron
shared_nts = get_shared_nucleotides_at_splicesite(
orfA, orfD, acceptor, donor)
# make a IntronConnectingOrfs object
intron = IntronConnectingOrfs(donor, acceptor, shared_nts, orfD,
orfA)
introns.append(intron)
# return ordered intron list
return _order_intron_list(introns, order_by=order_by)
def merge_orfs_with_tinyexon(
preceding_orf,
subsequent_orf,
preceding_donor_sites=[],
subsequent_acceptor_sites=[],
orflist=[],
max_tinyexon_nt_length=TINYEXON_MAX_NT_LENGTH,
min_tinyexon_nt_length=TINYEXON_MIN_NT_LENGTH,
max_tinyexon_intron_nt_length=TINYEXON_MAX_INTRON_NT_LENGTH,
min_tinyexon_intron_nt_length=TINYEXON_MIN_INTRON_NT_LENGTH,
min_donor_pssm_score=TINYEXON_MIN_DONOR_PSSM_SCORE,
min_acceptor_pssm_score=TINYEXON_MIN_ACCEPTOR_PSSM_SCORE,
min_total_pssm_score=TINYEXON_MIN_TOTAL_PSSM_SCORE,
**kwargs):
"""
Bridge two `neighbouring` Orfs by a tinyexon by applying preceding donors and subsequent acceptors
@type preceding_orf: Orf object
@param preceding_orf: Orf object that contains preceding_donor_site(s)
@type subsequent_orf: Orf object
@param subsequent_orf: Orf object that contains subsequent_acceptor_site(s)
@type preceding_donor_sites: list
@param preceding_donor_sites: list with SpliceDonorGT and/or SpliceDonor objects
@type subsequent_acceptor_sites: list
@param subsequent_acceptor_sites: list with SpliceAcceptorAG and/or SpliceAcceptor objects
@type orflist: list
@param orflist: list with Orf objects
@type max_tinyexon_nt_length: integer
@param max_tinyexon_nt_length: positive integer, largest length of tinyexon in nt
@type min_tinyexon_nt_length: integer
@param min_tinyexon_nt_length: positive integer, smallest length of tinyexon in nt
@type max_tinyexon_intron_nt_length: integer
@param max_tinyexon_intron_nt_length: positive integer, largest length of intron around tinyexon in nt
@type min_tinyexon_intron_nt_length: integer
@param min_tinyexon_intron_nt_length: positive integer, smallest length of intron around tinyexon in nt
@type min_total_pssm_score: float or None
@param min_total_pssm_score: minimal sum of donor - acceptor pssm score pair of tinyexon
@type min_donor_pssm_score: float or None
@param min_donor_pssm_score: minimal donor pssm score of tinyexon
@type min_acceptor_pssm_score: float or None
@param min_acceptor_pssm_score: minimal acceptor pssm score of tinyexon
@rtype: list
@return: list of tuples ( preceding_intron, tinyexon, subsequent_intron )
@attention: Global vars that have to be set upon usage:
MIN_DONOR_PSSM_SCORE
MIN_ACCEPTOR_PSSM_SCORE
# and all TINYEXON variable named
TINYEXON_MAX_NT_LENGTH
TINYEXON_MIN_NT_LENGTH
TINYEXON_MAX_INTRON_NT_LENGTH
TINYEXON_MIN_INTRON_NT_LENGTH
TINYEXON_MIN_PSSM_SCORE
TINYEXON_MIN_DONOR_PSSM_SCORE
TINYEXON_MIN_ACCEPTOR_PSSM_SCORE
TINYEXON_ALLOW_NON_CANONICAL_DONOR
TINYEXON_ALLOW_NON_CANONICAL_ACCEPTOR
TINYEXON_NON_CANONICAL_MIN_PSSM_SCORE
TINYEXON_NON_CANONICAL_MIN_DONOR_PSSM_SCORE
TINYEXON_NON_CANONICAL_MIN_ACCEPTOR_PSSM_SCORE
"""
if not preceding_donor_sites:
return []
if not subsequent_acceptor_sites:
return []
if not orflist:
return []
# return list with (intron,tinyexon,intron) tuples
returnexons = []
min_preceding_donor_sites_pos = min([d.pos for d in preceding_donor_sites])
max_subsequent_acceptor_sites_pos = max(
[a.pos for a in subsequent_acceptor_sites])
for orfX in orflist:
# check if orf is correctly positions towards the splice sites' extremes
if orfX.endPY <= min_preceding_donor_sites_pos: continue
if orfX.startPY >= max_subsequent_acceptor_sites_pos: continue
# if here, we can try to make a bridge by a tinyexon
for donor in preceding_donor_sites:
# orf not correctly positions towards the donor site
if orfX.endPY <= donor.pos: continue
# check pssm_score of donor site
# TODO: this is in fact the donor on the normal, large orf
# TODO: do we want to check this pssm score?
if donor.pssm_score < min_donor_pssm_score: continue
for acceptor in subsequent_acceptor_sites:
if orfX.startPY >= acceptor.pos: continue
# check pssm_score of acceptor site
# TODO: this is in fact the acceptor on the normal, large orf
# TODO: do we want to check this pssm score?
if acceptor.pssm_score < min_acceptor_pssm_score: continue
# okay, now try to bridge it!
exons = find_tiny_exon_on_orf(
orfX,
order_by='total_pssm',
max_tinyexon_nt_length=max_tinyexon_nt_length,
min_tinyexon_nt_length=min_tinyexon_nt_length,
max_tinyexon_intron_nt_length=max_tinyexon_intron_nt_length,
min_tinyexon_intron_nt_length=min_tinyexon_intron_nt_length,
min_donor_pssm_score=min_donor_pssm_score,
min_acceptor_pssm_score=min_acceptor_pssm_score,
min_total_pssm_score=min_total_pssm_score,
preceding_donor=donor,
subsequent_acceptor=acceptor)
# and append to returnexons
for tinyexon in exons:
# make preceding intron
shared_nts_A = get_shared_nucleotides_at_splicesite(
tinyexon.orf, preceding_orf, tinyexon.acceptor, donor)
preceding_intron = IntronConnectingOrfs(
donor, tinyexon.acceptor, shared_nts_A, preceding_orf,
tinyexon.orf)
# make subsequent intron
shared_nts_B = get_shared_nucleotides_at_splicesite(
subsequent_orf, tinyexon.orf, acceptor, tinyexon.donor)
subsequent_intron = IntronConnectingOrfs(
tinyexon.donor, acceptor, shared_nts_B, tinyexon.orf,
subsequent_orf)
# and append to exons
returnexons.append(
(preceding_intron, tinyexon, subsequent_intron))
# and return the list of intron/exon/intron
return returnexons