ycenter = random.randint(0, height)
# discard positions that would generate overlapping cells
if not cellOverlap(xcenter, ycenter, celldiam, centers):
centers.append([xcenter, ycenter])
# print("Spot "+str(count)+" center: "+str(centers[count]))
count += 1
# work on a copy of the image
from ij.plugin import Duplicator
imp2 = Duplicator().run(imp, 1, channels, 1, 1, 1, 1)
# clear the existing channel
imp2.setDisplayMode(IJ.COLOR)
imp2.setC(targetChannel)
IJ.run(imp2, "Select All", "")
IJ.setBackgroundColor(0, 0, 0)
IJ.run(imp2, "Clear", "slice")
# draw white circles to simulate cells
IJ.setForegroundColor(255, 255, 255)
for point in centers:
drawSpot(point, celldiam)
# save the simulated image
IJ.saveAs(imp2, "Tiff",
os.path.join(srcDir, basename + " sim " + str(trial) + ".tif"))
imp2.close()
