args = parser.parse_args() tmp_dir = imagej2_base_utils.get_temp_dir() source_image_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.source_image, args.source_image_format ) target_image_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.target_image, args.target_image_format ) source_elastic_transformation_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.source_elastic_transformation, 'txt' ) target_raw_transformation_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.target_raw_transformation, 'txt' ) # Define command response buffers. tmp_out = tempfile.NamedTemporaryFile().name tmp_stdout = open( tmp_out, 'wb' ) tmp_err = tempfile.NamedTemporaryFile().name tmp_stderr = open( tmp_err, 'wb' ) # Build the command line to compose the raw and elastic transformations. cmd = imagej2_base_utils.get_base_cmd_bunwarpj( None ) if cmd is None: imagej2_base_utils.stop_err( "bUnwarpJ not found!" ) cmd += ' -compose_raw_elastic' # Target is sent before source. cmd += ' %s' % target_image_path cmd += ' %s' % source_image_path cmd += ' %s' % target_raw_transformation_path cmd += ' %s' % source_elastic_transformation_path cmd += ' %s' % args.output # Compose the raw and elastic transformations into another raw transformation using bUnwarpJ. proc = subprocess.Popen( args=cmd, stderr=subprocess.PIPE, stdout=subprocess.PIPE, shell=True ) rc = proc.wait() if rc != 0: error_message = imagej2_base_utils.get_stderr_exception( tmp_err, tmp_stderr, tmp_out, tmp_stdout )
source_file_name = imagej2_base_utils.get_file_name_without_extension( tmp_source_out_tiff_path ) tmp_source_out_transf_path = os.path.join( tmp_dir, '%s_transf.txt' % source_file_name ) target_file_name = imagej2_base_utils.get_file_name_without_extension( tmp_target_out_tiff_path ) tmp_target_out_transf_path = os.path.join( tmp_dir, '%s_transf.txt' % target_file_name ) # Set the size of the memory allocation pool used by the JVM. memory_size = imagej2_base_utils.get_max_heap_size_value( args.max_heap_size_type, args.max_heap_size ) # Define command response buffers. tmp_out = tempfile.NamedTemporaryFile().name tmp_stdout = open( tmp_out, 'wb' ) tmp_err = tempfile.NamedTemporaryFile().name tmp_stderr = open( tmp_err, 'wb' ) # Build the command line to align the two images. cmd = imagej2_base_utils.get_base_cmd_bunwarpj( memory_size ) if cmd is None: imagej2_base_utils.stop_err( "bUnwarpJ not found!" ) cmd += ' -align' # Target is sent before source. cmd += ' %s' % target_image_path if args.target_mask is None: target_mask_str = ' NULL' else: target_mask_str = ' %s' % tmp_target_mask_path cmd += target_mask_str cmd += ' %s' % source_image_path if args.source_mask is None: source_mask_str = ' NULL' else: source_mask_str = ' %s' % tmp_source_mask_path