cell_concentration=cell_concentration,
buffer_source=buffer_trough,
cell_volume=cell_volume)
# optimize the syringe_concentration using heuristic equations and known binding constants
# TODO extract m, v and V0 from protocol somehow?
# Warning, you're possibly not getting the setup you want. Consider not using the Heuristic Experiment
experiment.heuristic_syringe(ligand_ka, 10, strict=False)
# rescale if syringe > stock. Store factor.
factors.append(experiment.rescale())
ligand_protein_experiments.append(experiment)
# Define corresponding ligand into buffer experiments
for experiment in ligand_protein_experiments:
experiment = copy.deepcopy(experiment)
experiment.cell_source = buffer_trough
experiment.cell_concentration *= 0
ligand_buffer_experiments.append(experiment)
# TODO, since we are changing ligands, we'd have to wash the syringe.
# Add ligand to protein experiment(s) to set
for ligand_protein_experiment in ligand_protein_experiments:
itc_experiment_set.addExperiment(ligand_protein_experiment)
# pprint.pprint(ligand_protein_experiment.__dict__)
# Add ligand_to_buffer experiment(s) to set
for ligand_buffer_experiment in ligand_buffer_experiments:
itc_experiment_set.addExperiment(ligand_buffer_experiment)
# pprint.pprint(ligand_buffer_experiment.__dict__)
# Add cleaning experiment.
cell_concentration=cell_concentration,
buffer_source=caii_buffer_trough,
cell_volume=cell_volume)
# optimize the syringe_concentration using heuristic equations and known binding constants
# TODO extract m, v and V0 from protocol somehow?
# Warning, you're possibly not getting the setup you want. Consider not using the Heuristic Experiment
experiment.heuristic_syringe(ligand_ka, 10, strict=False)
# rescale if syringe > stock. Store factor.
factors.append(experiment.rescale())
ligand_protein_experiments.append(experiment)
# Define corresponding ligand into buffer experiments
for experiment in ligand_protein_experiments:
experiment = copy.deepcopy(experiment)
experiment.cell_source = caii_buffer_trough
experiment.cell_concentration *= 0
ligand_buffer_experiments.append(experiment)
# TODO, since we are changing ligands, we'd have to wash the syringe.
# Add ligand to protein experiment(s) to set
for ligand_protein_experiment in ligand_protein_experiments:
itc_experiment_set.addExperiment(ligand_protein_experiment)
# pprint.pprint(ligand_protein_experiment.__dict__)
# Add ligand_to_buffer experiment(s) to set
for ligand_buffer_experiment in ligand_buffer_experiments:
itc_experiment_set.addExperiment(ligand_buffer_experiment)
# pprint.pprint(ligand_buffer_experiment.__dict__)
# Add cleaning experiment.
