pCAPs_EcoRV_ScENO2tp = (pYPKa_EcoRV + ScENO2tp).looped()
ScRPE1 = pcr( primer[556], primer[557], Dseqrecord(sgd.cds("RPE1")))
pCAPs_AjiI_ScRPE1 = (pYPKa_AjiI + ScRPE1).looped()
ScRKI1 = pcr( primer[555], primer[554], Dseqrecord(sgd.cds("RKI1")))
pCAPs_AjiI_ScRKI1 = (pYPKa_AjiI + ScRKI1).looped()
CiGXF1 = pcr( primer[553], primer[552], Dseqrecord(gb("AJ937350")))
pCAPs_AjiI_CiGXF1 = (pYPKa_AjiI + CiGXF1).looped()
from pYPK0 import pYPK0 as pCAPs_pSU0
pCAPs_pSU0_E_Z, stuffer = pCAPs_pSU0.cut((EcoRV,ZraI))
ypk_primer = parse("/home/bjorn/Dropbox/wikidata/ypk_primers.wiki")
#ScPDC1_PacI-ScTKL1-ScRPS19b_PacI
A_ScPDC1tp_b = pcr( primer[167], primer[567], pCAPs_ZraI_ScPDC1tp)
ScTKL1 = pcr( ypk_primer[8], ypk_primer[9], Dseqrecord(sgd.cds("TKL1")))
C_ScRPS19btp_d = pcr( primer[568], primer[166], pCAPs_EcoRV_ScRPS19btp)
circular = Assembly(( A_ScPDC1tp_b,
ScTKL1,
C_ScRPS19btp_d,
# RPS19atp_ScRKI1tp_TPI1tp
# TPI1tp_CiGXF1_ENO2tp
from pYPK0_PDC1tp_ScTKL1_RPS19btp import pYPK0_PDC1tp_ScTKL1_RPS19btp as cas1
from pYPK0_RPS19btp_ScRPE1_RPS19atp import pYPK0_RPS19btp_ScRPE1_RPS19atp as cas2
from pYPK0_RPS19atp_ScRKI1_TPI1tp import pYPK0_RPS19atp_ScRKI1_TPI1tp as cas3
from pYPK0_TPI1tp_CiGXF1_ENO2tp import pYPK0_TPI1tp_CiGXF1_ENO2tp as cas4
cas1 = pcr( p167, p647, cas1)
cas2 = pcr( p648, p651, cas2)
cas3 = pcr( p538, p621, cas3)
cas4 = pcr( p419, p166, cas4)
pYPK0_E_Z, stuffer = pYPK0.cut((EcoRV, ZraI))
#print [cas1, cas2, cas3, cas4, pYPK0_E_Z]
a = Assembly([cas1, cas2, cas3, cas4, pYPK0_E_Z], limit = 61)
seq = a.circular_products[0]
print seq.small_fig()
seq=seq.synced("tcgcgcgtttcggtgatgacggtgaaaacctctg")
pYPK0_T=seq
'''
# coding: utf-8
