def get_argument_parser():
    ap = get_default_argparser_with_base_opts(get_version(),
                                              "Toolkit for cDNA analysis.",
                                              default_level="WARN")
    subparsers = ap.add_subparsers(dest="subCommand")
    arg_parser = subparsers.add_parser(
        'classify',
        description="Classify reads based on whether they are " +
        "non-chimeric, full length and have their 5', " +
        "3' and poly A tail seen.")
    # Add arguments for subcommand classify
    add_classify_arguments(_wrap_parser(arg_parser))

    arg_parser = subparsers.add_parser(
        'cluster',
        description='Discover consensus isoforms based on ' +
        'quality controlled non-chimeric, ' +
        'full length reads to reference genome.')
    # Add arguments for subcommand cluster
    add_cluster_arguments(_wrap_parser(arg_parser))

    arg_parser = subparsers.add_parser(
        'subset', description='Subset annotated reads in FASTA format.')
    add_subset_arguments(_wrap_parser(arg_parser))
    ap.add_argument("--profile",
                    action="store_true",
                    help="Print runtime profile at exit")
    return ap
def get_argument_parser():
    ap = get_default_argparser_with_base_opts(get_version(),
        "Toolkit for cDNA analysis.", default_level="WARN")
    subparsers = ap.add_subparsers(dest="subCommand")
    arg_parser = subparsers.add_parser(
        'classify',
        description="Classify reads based on whether they are " +
                    "non-chimeric, full length and have their 5', " +
                    "3' and poly A tail seen.")
    # Add arguments for subcommand classify
    add_classify_arguments(_wrap_parser(arg_parser))

    arg_parser = subparsers.add_parser(
        'cluster',
        description='Discover consensus isoforms based on ' +
                    'quality controlled non-chimeric, ' +
                    'full length reads to reference genome.')
    # Add arguments for subcommand cluster
    add_cluster_arguments(_wrap_parser(arg_parser))

    arg_parser = subparsers.add_parser(
        'subset',
        description='Subset annotated reads in FASTA format.')
    add_subset_arguments(_wrap_parser(arg_parser))
    ap.add_argument(
        "--profile", action="store_true",
        help="Print runtime profile at exit")
    return ap
class Constants(object):
    """Constants used in pbtranscript.tasks.gather_gmap_sam"""
    TOOL_ID = "pbtranscript.tasks.gather_gmap_sam"
    VERSION = get_version()
    DRIVER = "python -m %s --resolved-tool-contract " % TOOL_ID
    PARSER_DESC = __doc__
    CHUNK_KEY = "$chunk.sam_id"
Exemple #4
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def get_parser():
    """Returns arg parser."""
    parser = argparse.ArgumentParser(prog='tofu_wrap')

    helpstr = "Input full-length non-chimeric reads in FASTA or ContigSet format " + \
              "(e.g., isoseq_flnc.fasta|contigset.xml)"
    parser.add_argument("flnc_fa", type=str, help=helpstr)
    helpstr = "Output collapsed filtered isoforms in FASTA/FASTQ format (e.g., tofu_out.fastq)"
    parser.add_argument("collapsed_filtered_fn", type=str, help=helpstr)

    parser = add_nfl_fa_argument(parser, positional=False, required=True)
    parser.add_argument("--nfl_reads_per_split", type=int,
                        dest="nfl_reads_per_split", default=60000,
                        help="Number of nFL reads per split file (default: 60000)")
    parser = add_fofn_arguments(parser, ccs_fofn=True, bas_fofn=True, fasta_fofn=True)

    # tofu output arguments
    parser = add_tofu_output_arguments(parser)

    parser = add_ice_arguments(parser) # Add Ice options, including --quiver
    parser = add_sge_arguments(parser, blasr_nproc=True, quiver_nproc=True, gcon_nproc=True) # Sge
    parser = add_ice_post_quiver_hq_lq_qv_arguments(parser) # IceQuiver HQ/LQ QV options.

    parser = add_separate_flnc_arguments(parser) # separate_flnc options
    parser = add_gmap_arguments(parser) # map to gmap reference options
    parser = add_post_mapping_to_genome_arguments(parser) # post mapping to genome options

    misc_group = parser.add_argument_group("Misc arguments")
    misc_group.add_argument("--mem_debug", default=False, action="store_true",
                            help=argparse.SUPPRESS)
    misc_group.add_argument("--keep_tmp_files", default=False, action="store_true",
                            help="False: delete tmp files; True: keep tmp files (default: False).")
    misc_group.add_argument("--version", action='version', version='%(prog)s ' + str(get_version()))
    return parser
Exemple #5
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def get_parser():
    """Returns arg parser."""
    parser = argparse.ArgumentParser(prog='tofu_wrap')

    helpstr = "Input full-length non-chimeric reads in FASTA or ContigSet format " + \
              "(e.g., isoseq_flnc.fasta|contigset.xml)"
    parser.add_argument("flnc_fa", type=str, help=helpstr)
    helpstr = "Output collapsed filtered isoforms in FASTA/FASTQ format (e.g., tofu_out.fastq)"
    parser.add_argument("collapsed_filtered_fn", type=str, help=helpstr)

    parser = add_nfl_fa_argument(parser, positional=False, required=True)
    parser.add_argument("--nfl_reads_per_split", type=int,
                        dest="nfl_reads_per_split", default=60000,
                        help="Number of nFL reads per split file (default: 60000)")
    parser = add_fofn_arguments(parser, ccs_fofn=True, bas_fofn=True, fasta_fofn=True)

    # tofu output arguments
    parser = add_tofu_output_arguments(parser)

    parser = add_ice_arguments(parser) # Add Ice options, including --quiver
    parser = add_sge_arguments(parser, blasr_nproc=True, quiver_nproc=True, gcon_nproc=True) # Sge
    parser = add_ice_post_quiver_hq_lq_qv_arguments(parser) # IceQuiver HQ/LQ QV options.

    parser = add_separate_flnc_arguments(parser) # separate_flnc options
    parser = add_gmap_arguments(parser) # map to gmap reference options
    parser = add_post_mapping_to_genome_arguments(parser) # post mapping to genome options

    misc_group = parser.add_argument_group("Misc arguments")
    misc_group.add_argument("--mem_debug", default=False, action="store_true",
                            help=argparse.SUPPRESS)
    misc_group.add_argument("--keep_tmp_files", default=False, action="store_true",
                            help="False: delete tmp files; True: keep tmp files (default: False).")
    misc_group.add_argument("--version", action='version', version='%(prog)s ' + str(get_version()))
    return parser
    def run(self):
        """Execute ice_partial.py all|split|i|merge."""
        cmd = self.args.subCommand
        logging.info("Running {f} {cmd} v{v}.".format(f=op.basename(__file__),
                                                      cmd=cmd,
                                                      v=get_version()))
        cmd_str = ""
        try:
            args = self.args
            obj = None
            if cmd == "all":
                sge_opts = SgeOptions(unique_id=args.unique_id,
                                      use_sge=args.use_sge,
                                      max_sge_jobs=args.max_sge_jobs,
                                      blasr_nproc=args.blasr_nproc)
                obj = IceAllPartials(
                    root_dir=args.root_dir,
                    fasta_filenames=args.fasta_filenames.split(','),
                    ref_fasta=args.ref_fasta,
                    out_pickle=args.out_pickle,
                    sge_opts=sge_opts,
                    ccs_fofn=args.ccs_fofn,
                    tmp_dir=args.tmp_dir)
            elif cmd == "one":
                # Only assign nfl reads in the given input_fasta file to isoforms
                obj = IcePartialOne(input_fasta=args.input_fasta,
                                    ref_fasta=args.ref_fasta,
                                    out_pickle=args.out_pickle,
                                    ccs_fofn=args.ccs_fofn,
                                    done_filename=args.done_filename,
                                    blasr_nproc=args.blasr_nproc,
                                    tmp_dir=args.tmp_dir)
            elif cmd == "split":
                obj = IcePartialSplit(root_dir=args.root_dir,
                                      nfl_fa=args.nfl_fa,
                                      N=args.N)
            elif cmd == "i":
                obj = IcePartialI(root_dir=args.root_dir,
                                  i=args.i,
                                  ccs_fofn=args.ccs_fofn,
                                  blasr_nproc=args.blasr_nproc,
                                  tmp_dir=args.tmp_dir)
            elif cmd == "merge":
                obj = IcePartialMerge(root_dir=args.root_dir, N=args.N)
            else:
                raise ValueError(
                    "Unknown command passed to {f}: {cmd}.".format(
                        f=op.basename(__file__), cmd=cmd))

            cmd_str = obj.cmd_str()
            logging.info("Running CMD: {cmd_str}".format(cmd_str=cmd_str))
            obj.run()
        except:
            logging.exception("Exiting {cmd_str} with return code 1.".format(
                cmd_str=cmd_str))
            return 1
        return 0
def get_base_contract_parser(Constants=BaseConstants, default_level="WARN"):
    p = get_pbparser(tool_id=Constants.TOOL_ID,
                     version=get_version(),
                     name=Constants.TOOL_ID,
                     description=Constants.PARSER_DESC,
                     driver_exe=Constants.DRIVER_EXE,
                     nproc=SymbolTypes.MAX_NPROC,
                     resource_types=(ResourceTypes.TMP_DIR, ),
                     default_level=default_level)
    return p
def get_base_contract_parser(Constants=BaseConstants, default_level="WARN"):
    p = get_pbparser(
        tool_id=Constants.TOOL_ID,
        version=get_version(),
        name=Constants.TOOL_ID,
        description=Constants.PARSER_DESC,
        driver_exe=Constants.DRIVER_EXE,
        nproc=SymbolTypes.MAX_NPROC,
        resource_types=(ResourceTypes.TMP_DIR,),
        default_level=default_level)
    return p
    def run(self):
        """Execute ice_partial.py all|one|split|i|merge."""
        cmd = self.args.subCommand
        logging.info("Running {f} {cmd} v{v}.".format(f=op.basename(__file__),
                                                      cmd=cmd, v=get_version()))
        cmd_str = ""
        try:
            args = self.args
            obj = None
            if cmd == "all":
                sge_opts = SgeOptions(unique_id=args.unique_id,
                                      use_sge=args.use_sge,
                                      max_sge_jobs=args.max_sge_jobs,
                                      blasr_nproc=args.blasr_nproc)
                obj = IceAllPartials(root_dir=args.root_dir,
                                     fasta_filenames=args.fasta_filenames.split(','),
                                     ref_fasta=args.ref_fasta,
                                     out_pickle=args.out_pickle,
                                     sge_opts=sge_opts,
                                     ccs_fofn=args.ccs_fofn,
                                     tmp_dir=args.tmp_dir)
            elif cmd == "one":
                # Only assign nfl reads in the given input_fasta file to isoforms
                obj = IcePartialOne(input_fasta=args.input_fasta,
                                    ref_fasta=args.ref_fasta,
                                    out_pickle=args.out_pickle,
                                    ccs_fofn=args.ccs_fofn,
                                    done_filename=args.done_filename,
                                    blasr_nproc=args.blasr_nproc,
                                    tmp_dir=args.tmp_dir)
            elif cmd == "split":
                obj = IcePartialSplit(root_dir=args.root_dir,
                                      nfl_fa=args.nfl_fa,
                                      N=args.N)
            elif cmd == "i":
                obj = IcePartialI(root_dir=args.root_dir, i=args.i,
                                  ccs_fofn=args.ccs_fofn,
                                  blasr_nproc=args.blasr_nproc,
                                  tmp_dir=args.tmp_dir)
            elif cmd == "merge":
                obj = IcePartialMerge(root_dir=args.root_dir,
                                      N=args.N)
            else:
                raise ValueError("Unknown command passed to {f}: {cmd}.".
                                 format(f=op.basename(__file__), cmd=cmd))

            cmd_str = obj.cmd_str()
            logging.info("Running CMD: {cmd_str}".format(cmd_str=cmd_str))
            obj.run()
        except:
            logging.exception("Exiting {cmd_str} with return code 1.".
                              format(cmd_str=cmd_str))
            return 1
        return 0
Exemple #10
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def main():
    """Main function, split a fasta into smaller chunks."""
    import logging
    from pbtranscript.__init__ import get_version
    log = logging.getLogger(__name__)
    args = get_args()
    from pbtranscript.Utils import setup_log
    setup_log(alog=log, level=logging.DEBUG)
    log.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                        v=get_version()))

    splitFasta(input_fasta=args.input_fasta,
               reads_per_split=args.reads_per_split,
               out_dir=args.out_dir,
               out_prefix=args.out_prefix)
 def run(self):
     """Run"""
     logging.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                             v=get_version()))
     args = self.args
     try:
         convert_fofn_to_fasta(fofn_filename=args.input_fofn,
                               out_filename=args.fasta_fofn,
                               fasta_out_dir=args.fasta_out_dir,
                               force_overwrite=False)
     except:
         logging.exception("Failed to convert fofn {f} to fasta.".format(
             f=args.input_fofn))
         return 1
     return 0
 def run(self):
     """Run"""
     logging.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                             v=get_version()))
     args = self.args
     try:
         convert_fofn_to_fasta(fofn_filename=args.input_fofn,
                               out_filename=args.fasta_fofn,
                               fasta_out_dir=args.fasta_out_dir,
                               force_overwrite=False)
     except:
         logging.exception("Failed to convert fofn {f} to fasta.".
                           format(f=args.input_fofn))
         return 1
     return 0
 def run(self):
     """Run"""
     logging.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                             v=get_version()))
     cmd_str = ""
     try:
         args = self.args
         iceqm = IceQuiverMerge(root_dir=args.root_dir, N=args.N)
         cmd_str = iceqm.cmd_str()
         iceqm.run()
     except:
         logging.exception("Exiting {cmd_str} with return code 1.".
                           format(cmd_str=cmd_str))
         return 1
     return 0
Exemple #14
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def main():
    """Main function, split a fasta into smaller chunks."""
    import logging
    from pbtranscript.__init__ import get_version
    log = logging.getLogger(__name__)
    args = get_args()
    from pbtranscript.Utils import setup_log
    setup_log(alog=log, level=logging.DEBUG)
    log.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                        v=get_version()))

    splitFasta(input_fasta=args.input_fasta,
               reads_per_split=args.reads_per_split,
               out_dir=args.out_dir,
               out_prefix=args.out_prefix)
 def run(self):
     """Run"""
     logging.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                             v=get_version()))
     cmd_str = ""
     try:
         args = self.args
         iceqm = IceQuiverMerge(root_dir=args.root_dir, N=args.N)
         cmd_str = iceqm.cmd_str()
         iceqm.run()
     except:
         logging.exception("Exiting {cmd_str} with return code 1.".
                           format(cmd_str=cmd_str))
         return 1
     return 0
def set_parser():
    """Set up and return argument parser."""
    parser = ArgumentParser()
    parser.add_argument("input_fasta",
                        help="Input fasta filename")
    parser.add_argument("output_prefix",
                        help="Output filename prefix (ex: g_consensus)")
    parser.add_argument("consensus_id",
                        help="Consensus sequence ID name (ex: consensus)")
    parser.add_argument("--nproc",
                        default=8, type=int,
                        help="Number of processes")
    parser.add_argument("--maxScore", default=-1000, type=int,
                        help="blasr maxScore")
    parser.add_argument("--version", "-v",
                        action='version', version='%(prog)s ' + get_version())
    return parser
Exemple #17
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def set_parser():
    """Set up and return argument parser."""
    parser = ArgumentParser()
    parser.add_argument("input_fasta", help="Input fasta filename")
    parser.add_argument("output_prefix",
                        help="Output filename prefix (ex: g_consensus)")
    parser.add_argument("consensus_id",
                        help="Consensus sequence ID name (ex: consensus)")
    parser.add_argument("--nproc",
                        default=8,
                        type=int,
                        help="Number of processes")
    parser.add_argument("--maxScore",
                        default=-1000,
                        type=int,
                        help="blasr maxScore")
    parser.add_argument("--version",
                        "-v",
                        action='version',
                        version='%(prog)s ' + get_version())
    return parser
Exemple #18
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    def run(self):
        """Execute ice_fa2fq.py."""
        logging.info("Running {f} v{v}.".format(f=op.basename(__file__),
                                                v=get_version()))
        cmd_str = ""
        try:
            args = self.args
            in_fa, ccs_fofn, out_fq = args.in_fa, args.ccs_fofn, \
                                      args.out_fq

            self.validate_inputs(in_fa=in_fa, ccs_fofn=ccs_fofn)

            cmd_str = self.cmd_str(in_fa=in_fa,
                                   ccs_fofn=ccs_fofn,
                                   out_fq=out_fq)

            ice_fa2fq(in_fa=in_fa, ccs_fofn=ccs_fofn, out_fq=out_fq)

        except:
            logging.exception(
                "Exiting {cmd} with return code 1.".format(cmd=cmd_str))
            return 1
        return 0
Exemple #19
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 def _logConfigs(self):
     """Log configuration."""
     with open(self.configFN, 'w', 0) as f:
         f.write('pbtranscript ' + get_version() + "\n")
         f.write(str(self.ice_opts) + "\n")
         f.write(str(self.sge_opts) + "\n")
 def getVersion(self):
     """Return version string."""
     return get_version()
 def getVersion(self):
     return get_version()
Exemple #22
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 def run(self):
     """Execute ice_quiver.py all|i|merge|postprocess."""
     cmd = self.args.subCommand
     logging.info("Running {f} {cmd} v{v}.".format(f=op.basename(__file__),
                                                   cmd=cmd,
                                                   v=get_version()))
     cmd_str = ""
     try:
         args = self.args
         obj = None
         if cmd == "all":
             sge_opts = SgeOptions2(unique_id=args.unique_id,
                                    use_sge=args.use_sge,
                                    max_sge_jobs=args.max_sge_jobs,
                                    blasr_nproc=args.blasr_nproc,
                                    arrow_nproc=args.arrow_nproc,
                                    sge_env_name=args.sge_env_name,
                                    sge_queue=args.sge_queue,
                                    qsub_extra=args.qsub_extra)
             ipq_opts = IceArrowHQLQOptions2(
                 hq_isoforms_fa=args.hq_isoforms_fa,
                 hq_isoforms_fq=args.hq_isoforms_fq,
                 lq_isoforms_fa=args.lq_isoforms_fa,
                 lq_isoforms_fq=args.lq_isoforms_fq,
                 qv_trim_5=args.qv_trim_5,
                 qv_trim_3=args.qv_trim_3,
                 hq_arrow_min_accuracy=args.hq_arrow_min_accuracy)
             obj = IceArrowAll2(root_dir=args.root_dir,
                                subread_xml=args.subread_xml,
                                sge_opts=sge_opts,
                                ipq_opts=ipq_opts,
                                tmp_dir=args.tmp_dir)
         # elif cmd == "i":
         #     sge_opts = SgeOptions(unique_id=args.unique_id,
         #                           use_sge=args.use_sge,
         #                           max_sge_jobs=args.max_sge_jobs,
         #                           blasr_nproc=args.blasr_nproc,
         #                           arrow_nproc=args.arrow_nproc)
         #     obj = IceQuiverI(root_dir=args.root_dir, i=args.i, N=args.N,
         #                      bas_fofn=args.bas_fofn,
         #                      fasta_fofn=None,
         #                      sge_opts=sge_opts,
         #                      tmp_dir=args.tmp_dir)
         #elif cmd == "merge":
         #    obj = IceQuiverMerge(root_dir=args.root_dir, N=args.N)
         elif cmd == "postprocess":
             ipq_opts = IceArrowHQLQOptions2(
                 hq_isoforms_fa=args.hq_isoforms_fa,
                 hq_isoforms_fq=args.hq_isoforms_fq,
                 lq_isoforms_fa=args.lq_isoforms_fa,
                 lq_isoforms_fq=args.lq_isoforms_fq,
                 qv_trim_5=args.qv_trim_5,
                 qv_trim_3=args.qv_trim_3,
                 hq_arrow_min_accuracy=args.hq_arrow_min_accuracy,
                 hq_min_full_length_reads=args.hq_min_full_length_reads)
             obj = IceArrowPostProcess2(
                 root_dir=args.root_dir,
                 ipq_opts=ipq_opts,
                 quit_if_not_done=args.quit_if_not_done,
                 summary_fn=args.summary_fn,
                 report_fn=args.report_fn)
         else:
             raise ValueError(
                 "Unknown command passed to {f}: {cmd}.".format(
                     f=op.basename(__file__), cmd=cmd))
         cmd_str = obj.cmd_str()
         logging.info("Running CMD: {cmd_str}".format(cmd_str=cmd_str))
         obj.run()
     except:
         logging.exception("Exiting {cmd_str} with return code 1.".format(
             cmd_str=cmd_str))
         return 1
     return 0
    def run(self):
        """Execute ice_partial.py all|one|split|i|merge."""
        cmd = self.args.subCommand
        logging.info("Running {f} {cmd} v{v}.".format(f=op.basename(__file__),
                                                      cmd=cmd,
                                                      v=get_version()))
        cmd_str = ""
        try:
            args = self.args
            obj = None
            if cmd in ('all', 'one'):
                # currently user NOT allowed to set full missed start/end
                # (we also set it to 30/10 bp which is more stringent than in IceIterative2,
                # which is hard set to 50/30)
                ice_opts = IceOptions2(ece_penalty=args.ece_penalty,
                                       ece_min_len=args.ece_min_len,
                                       max_missed_start=args.max_missed_start,
                                       max_missed_end=args.max_missed_end,
                                       full_missed_start=30,
                                       full_missed_end=10,
                                       min_match_len=50,
                                       aligner_choice=args.aligner_choice)

            if cmd == "all":
                sge_opts = SgeOptions2(unique_id=args.unique_id,
                                       use_sge=args.use_sge,
                                       max_sge_jobs=args.max_sge_jobs,
                                       sge_queue=args.sge_queue,
                                       sge_env_name=args.sge_env_name,
                                       qsub_extra=args.qsub_extra)
                obj = IceAllPartials2(
                    root_dir=args.root_dir,
                    fasta_filenames=args.fasta_filenames.split(','),
                    fastq_filenames=args.fastq_filenames.split(',')
                    if args.fastq_filenames is not None else None,
                    ref_fasta=args.ref_fasta,
                    out_pickle=args.out_pickle,
                    ice_opts=ice_opts,
                    sge_opts=sge_opts,
                    cpus=args.cpus,
                    tmp_dir=args.tmp_dir)
            elif cmd == "one":
                # Only assign nfl reads in the given input_fasta file to isoforms
                # "one" is always run locally so no need for SGE option
                obj = IcePartialOne2(input_fasta=args.input_fasta,
                                     input_fastq=args.input_fastq,
                                     ref_fasta=args.ref_fasta,
                                     out_pickle=args.out_pickle,
                                     done_filename=args.done_filename,
                                     ice_opts=ice_opts,
                                     cpus=args.cpus,
                                     tmp_dir=args.tmp_dir)
            elif cmd == "split":
                obj = IcePartialSplit2(root_dir=args.root_dir,
                                       nfl_fa=args.nfl_fa,
                                       nfl_fq=args.nfl_fq,
                                       N=args.N)
            # elif cmd == "i":
            #     obj = IcePartialI(root_dir=args.root_dir, i=args.i,
            #                       ccs_fofn=args.ccs_fofn,
            #                       blasr_nproc=args.blasr_nproc,
            #                       tmp_dir=args.tmp_dir)
            elif cmd == "merge":
                obj = IcePartialMerge(root_dir=args.root_dir, N=args.N)
            else:
                raise ValueError(
                    "Unknown command passed to {f}: {cmd}.".format(
                        f=op.basename(__file__), cmd=cmd))

            cmd_str = obj.cmd_str()
            logging.info("Running CMD: {cmd_str}".format(cmd_str=cmd_str))
            obj.run()
        except:
            logging.exception("Exiting {cmd_str} with return code 1.".format(
                cmd_str=cmd_str))
            return 1
        return 0
Exemple #24
0
 def run(self):
     """Execute ice_quiver.py all|i|merge|postprocess."""
     cmd = self.args.subCommand
     logging.info("Running {f} {cmd} v{v}.".format(f=op.basename(__file__),
                                                   cmd=cmd, v=get_version()))
     cmd_str = ""
     try:
         args = self.args
         obj = None
         if cmd == "all":
             sge_opts = SgeOptions(unique_id=args.unique_id,
                                   use_sge=args.use_sge,
                                   max_sge_jobs=args.max_sge_jobs,
                                   blasr_nproc=args.blasr_nproc,
                                   quiver_nproc=args.quiver_nproc)
             ipq_opts = IceQuiverHQLQOptions(
                 hq_isoforms_fa=args.hq_isoforms_fa,
                 hq_isoforms_fq=args.hq_isoforms_fq,
                 lq_isoforms_fa=args.lq_isoforms_fa,
                 lq_isoforms_fq=args.lq_isoforms_fq,
                 qv_trim_5=args.qv_trim_5,
                 qv_trim_3=args.qv_trim_3,
                 hq_quiver_min_accuracy=args.hq_quiver_min_accuracy)
             obj = IceQuiverAll(root_dir=args.root_dir,
                                bas_fofn=args.bas_fofn,
                                fasta_fofn=None,
                                sge_opts=sge_opts,
                                ipq_opts=ipq_opts,
                                tmp_dir=args.tmp_dir)
         elif cmd == "i":
             sge_opts = SgeOptions(unique_id=args.unique_id,
                                   use_sge=args.use_sge,
                                   max_sge_jobs=args.max_sge_jobs,
                                   blasr_nproc=args.blasr_nproc,
                                   quiver_nproc=args.quiver_nproc)
             obj = IceQuiverI(root_dir=args.root_dir, i=args.i, N=args.N,
                              bas_fofn=args.bas_fofn,
                              fasta_fofn=None,
                              sge_opts=sge_opts,
                              tmp_dir=args.tmp_dir)
         elif cmd == "merge":
             obj = IceQuiverMerge(root_dir=args.root_dir, N=args.N)
         elif cmd == "postprocess":
             ipq_opts = IceQuiverHQLQOptions(
                 hq_isoforms_fa=args.hq_isoforms_fa,
                 hq_isoforms_fq=args.hq_isoforms_fq,
                 lq_isoforms_fa=args.lq_isoforms_fa,
                 lq_isoforms_fq=args.lq_isoforms_fq,
                 qv_trim_5=args.qv_trim_5,
                 qv_trim_3=args.qv_trim_3,
                 hq_quiver_min_accuracy=args.hq_quiver_min_accuracy)
             obj = IceQuiverPostprocess(root_dir=args.root_dir,
                                        ipq_opts=ipq_opts,
                                        use_sge=args.use_sge,
                                        quit_if_not_done=args.quit_if_not_done,
                                        summary_fn=args.summary_fn,
                                        report_fn=args.report_fn)
         else:
             raise ValueError("Unknown command passed to {f}: {cmd}.".
                              format(f=op.basename(__file__), cmd=cmd))
         cmd_str = obj.cmd_str()
         logging.info("Running CMD: {cmd_str}".format(cmd_str=cmd_str))
         obj.run()
     except:
         logging.exception("Exiting {cmd_str} with return code 1.".
                           format(cmd_str=cmd_str))
         return 1
     return 0