def test_long_right_primer_default_behavior(self, gfp):
design = Design()
design.settings.template(gfp)
design.settings.right_sequence(rc(gfp[-50:]))
design.PRIMER_MAX_TM.value = 75.0
design.PRIMER_MAX_SIZE = 35
design.PRIMER_PICK_ANYWAY = 1
with pytest.raises(Primer3PlusRunTimeError):
design.run_and_optimize(10)
def test_long_right_primer(self, gfp):
design = Design()
design.settings.template(gfp)
design.settings.right_sequence(rc(gfp[-50:]))
design.PRIMER_MAX_TM.value = 75.0
design.PRIMER_MAX_SIZE = 35
design.PRIMER_PICK_ANYWAY = 1
design.settings.long_ok()
pairs, explain = design.run_and_optimize(10)
print(explain)
assert pairs
def test_set_long_overhang(self, gfp):
design = Design()
design.settings.template(gfp)
design.settings.left_sequence(gfp[0:50])
design.PRIMER_MAX_TM.value = 75.0
design.PRIMER_MAX_SIZE = 35
design.PRIMER_PICK_ANYWAY = 1
design.settings.left_overhang("AAAAAAAAA")
design.settings.long_ok()
design.settings.use_overhangs()
design.settings.pick_anyway()
pairs, explain = design.run()
print(explain)
import json
print(json.dumps(pairs[0]["LEFT"], indent=1))
assert pairs