def test_getBeadsVolForAllSamples(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(1)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v_list = s._getBeadsVolForAllSamples(1)
self.assertEqual(v_list[0], 15)
self.assertEqual(v_list[1], 31)
self.assertEqual(v_list[2], 22)
self.prep_createBeadsVolSettings(2)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v_list = s._getBeadsVolForAllSamples(1)
self.assertEqual(v_list[0], 30)
self.assertEqual(v_list[1], 20)
self.assertEqual(v_list[2], 25)
v_list = s._getBeadsVolForAllSamples(2)
self.assertEqual(v_list[0], 15)
self.assertEqual(v_list[1], 31)
self.assertEqual(v_list[2], 22)
def test_getBeadsVolDirectly(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(2)
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
v = s._getBeadsVolDirectly(0, 1)
self.assertEqual(30, v)
v = s._getBeadsVolDirectly(1, 1)
self.assertEqual(20, v)
v = s._getBeadsVolDirectly(2, 1)
self.assertEqual(25, v)
v = s._getBeadsVolDirectly(0, 2)
self.assertEqual(15, v)
v = s._getBeadsVolDirectly(1, 2)
self.assertEqual(31, v)
v = s._getBeadsVolDirectly(2, 2)
self.assertEqual(22, v)
self.delTempSettingsFile()
del s
self. prep_createBeadsVolSettings(1)
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
v = s._getBeadsVolDirectly(0, 1)
self.assertEqual(15, v)
v = s._getBeadsVolDirectly(1, 1)
self.assertEqual(31, v)
v = s._getBeadsVolDirectly(2, 1)
self.assertEqual(22, v)
def test_getRackName(self, mock_loadIndividualSettings):
logging.disable(logging.CRITICAL)
beads = 'Beads tube rack'
waste = 'Waste tube rack'
eluent = 'Eluent tube rack'
ethanol = 'Ethanol tube rack'
self.prep_settingsFile(beads, ['reagents'])
self.prep_settingsFile(waste, ['reagents'])
self.prep_settingsFile(eluent, ['reagents'])
self.prep_settingsFile(ethanol, ['reagents'])
s = ponec.settings(self.mock_csv_path)
val = s._getRackName('Beads')
self.assertEqual('reagents', val)
val = s._getRackName('Waste')
self.assertEqual('reagents', val)
val = s._getRackName('Eluent')
self.assertEqual('reagents', val)
val = s._getRackName('Ethanol')
self.assertEqual('reagents', val)
self.delTempSettingsFile()
self.prep_settingsFile(beads, ['samples'])
s = ponec.settings(self.mock_csv_path)
val = s._getRackName('Beads')
self.assertEqual('samples', val)
self.delTempSettingsFile()
self.prep_settingsFile(beads, ['asdf'])
s = ponec.settings(self.mock_csv_path)
val = s._getRackName('Beads')
self.assertEqual(None, val)
self.delTempSettingsFile()
def test__getBeadsVolume_1stage(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(1)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, 15)
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, 31)
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, 22)
# Rewriting the settings file.
# Now the function should calculate volume from the initial volume and fraction
param = 'Beads volume'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, 100)
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, 29*0.9)
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, 50*1.5)
# Rewriting the settings file.
# Now the function should calculate volume from the DNA cutoff value
param = 'Fraction'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
a,b,c = bl.getBeadsVolumeCoef()
expected_dna_cutoff = 150
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 100 * f_expected
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, v_expected)
expected_dna_cutoff = 300
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 29 * f_expected
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, v_expected)
expected_dna_cutoff = 100
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 50 * f_expected
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, v_expected)
def setUp(self, mock_serial, mock_sleep, mock_sleep2, mock_sleep3, mock_writeAndWait):
logging.disable(logging.CRITICAL)
self.test_samplesheet_path = r'.\tests\samplesheet_protocol_test.csv'
self.test_samplesheet_path_2 = r'.\tests\samplesheet_2stages_protocol_test.csv'
copyfile(r'.\factory_default\samplesheet.csv', self.test_samplesheet_path)
copyfile(r'.\factory_default\samplesheet_2stages.csv', self.test_samplesheet_path_2)
self.ber = ponec.bl.robot(cartesian_port_name='COM1', loadcell_port_name='COM2')
self.settings = ponec.settings(self.test_samplesheet_path)
self.settings2 = ponec.settings(self.test_samplesheet_path_2)
# 11/12/2021 - default pipetting speed is 2500.0
self.default_pipetting_speed = self.ber.getSpeedPipette()
def test_getTubePositionInRack(self, mock_loadIndividualSettings):
self.prep_settingsFile('Beads tube column', [0])
self.prep_settingsFile('Beads tube well', [4])
s = ponec.settings(self.mock_csv_path)
col, row = s._getTubePositionInRack('Beads')
self.assertEqual(col, 0)
self.assertEqual(row, 4)
def test__beadsVolNotValid(self, mock_loadIndividualSettings):
self.createTempSettingsFile()
s = ponec.settings(self.mock_csv_path)
self.assertFalse(s._beadsVolNotValid(100))
self.assertFalse(s._beadsVolNotValid(947))
self.assertTrue(s._beadsVolNotValid(0))
self.assertTrue(s._beadsVolNotValid(None))
def test_getBeadsVolUsingDNACutoff(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(1)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
a,b,c = bl.getBeadsVolumeCoef()
f_expected = a + b / 150 + c / 150 ** 2
v = s._getBeadsVolUsingDNACutoff(0, 1)
self.assertEqual(v, f_expected*100)
self.assertFalse(v == 0)
f_expected = a + b / 300 + c / 300 ** 2
v = s._getBeadsVolUsingDNACutoff(1, 1)
self.assertEqual(v, f_expected*29)
f_expected = a + b / 100 + c / 100 ** 2
v = s._getBeadsVolUsingDNACutoff(2, 1)
self.assertEqual(v, f_expected*50)
self.delTempSettingsFile()
del s
self.prep_createBeadsVolSettings(2)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
f_expected = a + b / 900 + c / 900 ** 2
v = s._getBeadsVolUsingDNACutoff(0, 1)
self.assertEqual(v, f_expected*100)
f_expected = a + b / 800 + c / 800 ** 2
v = s._getBeadsVolUsingDNACutoff(1, 1)
self.assertEqual(v, f_expected*29)
f_expected = a + b / 700 + c / 700 ** 2
v = s._getBeadsVolUsingDNACutoff(2, 1)
self.assertEqual(v, f_expected*50)
def test_init(self, mock_loadIndividualSettings):
self.createTempSettingsFile()
s = ponec.settings(self.mock_csv_path)
for row in s.settings:
self.assertTrue('Parameters' in row)
self.assertTrue('0' in row)
param = row['Parameters']
if param == 'Number of cutoffs':
self.assertEqual(row['0'], '1')
def test_positionsToPurify2ndStage(self, mock_loadIndividualSettings):
self.prep_settingsFile('Initial sample volume',
[100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
positions = s.positionsToPurify2ndStage()
self.assertEqual(positions, [6, 7, 8])
s.cleanups = 2
positions = s.positionsToPurify2ndStage()
self.assertEqual(positions, [3, 4, 5])
self.delTempSettingsFile()
def test_calcBeadsVolFromFrac(self, mock_loadIndividualSettings):
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._calcBeadsVolFromFrac(0, 0.5)
self.assertEqual(v, 50)
v = s._calcBeadsVolFromFrac(2, 0.5)
self.assertEqual(v, 25)
def test_getBeadsSetting(self, mock_loadIndividualSettings):
self. prep_createBeadsVolSettings(1)
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
v = s._getBeadsSetting(0, 1, 'Beads volume')
self.assertEqual(15, v)
v = s._getBeadsSetting(1, 1, 'Beads volume')
self.assertEqual(31, v)
v = s._getBeadsSetting(2, 1, 'Beads volume')
self.assertEqual(22, v)
def test_returnPort(self, mock_loadIndividualSettings):
ls_param = 'Load cells controller port'
crt_param = 'Cartesian controller port'
self.prep_settingsFile(ls_param, ['auto'])
self.prep_settingsFile(crt_param, ['auto'])
s = ponec.settings(self.mock_csv_path)
ls_port = s.returnPort(kind=ls_param)
crt_port = s.returnPort(kind=crt_param)
self.assertEqual(ls_port, None)
self.assertEqual(crt_port, None)
self.delTempSettingsFile()
self.prep_settingsFile(ls_param, ['COM1'])
self.prep_settingsFile(crt_param, ['COM2'])
s = ponec.settings(self.mock_csv_path)
ls_port = s.returnPort(kind=ls_param)
crt_port = s.returnPort(kind=crt_param)
self.assertEqual(ls_port, 'COM1')
self.assertEqual(crt_port, 'COM2')
self.delTempSettingsFile()
def test_positionsToPurify(self, mock_loadIndividualSettings):
self.prep_settingsFile('Initial sample volume',
[100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
positions = s.positionsToPurify()
self.assertEqual(positions, [0, 1, 2])
self.delTempSettingsFile()
self.prep_settingsFile('Initial sample volume',
[-1, -1, 60, 20, 30, -1, -1, -1, 0, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
positions = s.positionsToPurify()
self.assertEqual(positions, [2, 3, 4])
self.delTempSettingsFile()
self.prep_settingsFile('Initial sample volume',
[0, 0, 0, 0, 0, -1, -1, -1, 0, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
positions = s.positionsToPurify()
self.assertEqual(positions, [])
self.delTempSettingsFile()
def test_getBeadsSetting_2stages(self, mock_loadIndividualSettings):
self. prep_createBeadsVolSettings(2)
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
v = s._getBeadsSetting(0, 1, 'Beads volume')
self.assertEqual(30, v)
v = s._getBeadsSetting(1, 1, 'Beads volume')
self.assertEqual(20, v)
v = s._getBeadsSetting(2, 1, 'Beads volume')
self.assertEqual(25, v)
self.delTempSettingsFile()
def test_getBeadsVolUsingFraction(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(1)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolUsingFraction(0, 1)
self.assertEqual(v, 100)
v = s._getBeadsVolUsingFraction(1, 1)
self.assertEqual(v, 29*0.9)
v = s._getBeadsVolUsingFraction(2, 1)
self.assertEqual(v, 50*1.5)
self.prep_createBeadsVolSettings(2)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolUsingFraction(0, 1)
self.assertEqual(v, 100*0.5)
v = s._getBeadsVolUsingFraction(1, 1)
self.assertEqual(v, 29*0.6)
v = s._getBeadsVolUsingFraction(2, 1)
self.assertEqual(v, 50*0.55)
v = s._getBeadsVolUsingFraction(0, 2)
self.assertEqual(v, 100*(1.0-0.5))
v = s._getBeadsVolUsingFraction(1, 2)
self.assertEqual(v, 29*(0.9-0.6))
v = s._getBeadsVolUsingFraction(2, 2)
self.assertEqual(v, 50*(1.5-0.55))
def test_getBeadsSetting_2stages_both(self, mock_loadIndividualSettings):
self. prep_createBeadsVolSettings(2)
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
# 1st stage
v = s._getBeadsSetting(0, 1, 'Beads volume')
self.assertEqual(30, v)
v = s._getBeadsSetting(1, 1, 'Beads volume')
self.assertEqual(20, v)
v = s._getBeadsSetting(2, 1, 'Beads volume')
self.assertEqual(25, v)
f = s._getBeadsSetting(0, 1, 'Fraction')
self.assertEqual(0.5, f)
f = s._getBeadsSetting(1, 1, 'Fraction')
self.assertEqual(0.6, f)
f = s._getBeadsSetting(2, 1, 'Fraction')
self.assertEqual(0.55, f)
dnasize = s._getBeadsSetting(0, 1, 'DNA size')
self.assertEqual(900, dnasize)
dnasize = s._getBeadsSetting(1, 1, 'DNA size')
self.assertEqual(800, dnasize)
dnasize = s._getBeadsSetting(2, 1, 'DNA size')
self.assertEqual(700, dnasize)
# 2nd stage
v = s._getBeadsSetting(0, 2, 'Beads volume')
self.assertEqual(15, v)
v = s._getBeadsSetting(1, 2, 'Beads volume')
self.assertEqual(31, v)
v = s._getBeadsSetting(2, 2, 'Beads volume')
self.assertEqual(22, v)
f = s._getBeadsSetting(0, 2, 'Fraction')
self.assertEqual(1.0, f)
f = s._getBeadsSetting(1, 2, 'Fraction')
self.assertEqual(0.9, f)
f = s._getBeadsSetting(2, 2, 'Fraction')
self.assertEqual(1.5, f)
dnasize = s._getBeadsSetting(0, 2, 'DNA size')
self.assertEqual(150, dnasize)
dnasize = s._getBeadsSetting(1, 2, 'DNA size')
self.assertEqual(300, dnasize)
dnasize = s._getBeadsSetting(2, 2, 'DNA size')
self.assertEqual(100, dnasize)
def test_calcBeadVolFractionFromDNACutoff(self, mock_loadIndividualSettings):
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
f = s._calcBeadVolFractionFromDNACutoff(1000)
a,b,c = bl.getBeadsVolumeCoef()
f_expected = a + b / 1000 + c / 1000 ** 2
self.assertEqual(f, f_expected)
f = s._calcBeadVolFractionFromDNACutoff(160)
a,b,c = bl.getBeadsVolumeCoef()
f_expected = a + b / 160 + c / 160 ** 2
self.assertEqual(f, f_expected)
def test_returnProtocolParameter(self, mock_loadIndividualSettings):
self.createTempSettingsFile()
s = ponec.settings(self.mock_csv_path)
val = s.returnProtocolParameter('Number of cutoffs')
self.assertEqual(val, 1)
def test_getRowWithParameter(self, mock_loadIndividualSettings):
self.createTempSettingsFile()
s = ponec.settings(self.mock_csv_path)
row = s.getRowWithParameter('Number of cutoffs')
self.assertEqual(row['0'], '1')
def setUp(self, mock_serial, mock_sleep, mock_sleep2, mock_sleep3, mock_writeAndWait):
logging.disable(logging.CRITICAL)
self.ber = ponec.bl.robot(cartesian_port_name='COM1', loadcell_port_name='COM2')
csv_path = r'.\factory_default\samplesheet.csv'
self.settings = ponec.settings(csv_path)
def test__getBeadsVolume_2stage(self, mock_loadIndividualSettings):
self.prep_createBeadsVolSettings(2)
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, 30)
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, 20)
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, 25)
v = s._getBeadsVolume(0, 2)
self.assertEqual(v, 15)
v = s._getBeadsVolume(1, 2)
self.assertEqual(v, 31)
v = s._getBeadsVolume(2, 2)
self.assertEqual(v, 22)
# Rewriting the settings file.
# Now the function should calculate volume from the initial volume and fraction
param = 'Beads volume'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
param = 'Beads volume upper cutoff'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, 100*0.5)
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, 29*0.6)
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, 50*0.55)
v = s._getBeadsVolume(0, 2)
self.assertEqual(v, 100*(1.0-0.5))
v = s._getBeadsVolume(1, 2)
self.assertEqual(v, 29*(0.9-0.6))
v = s._getBeadsVolume(2, 2)
self.assertEqual(v, 50*(1.5-0.55))
# Rewriting the settings file.
# Now the function should calculate volume from the initial volume and DNA cutoff
param = 'Fraction'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
param = 'Fraction upper cutoff'
self.prep_settingsFile(param, [-1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
s.cutoffs = s.returnProtocolParameter('Number of cutoffs')
s.initial_sample_vol_list = s._returnSampleParameterList('Initial sample volume')
a,b,c = bl.getBeadsVolumeCoef()
expected_dna_cutoff = 900
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 100 * f_expected
v = s._getBeadsVolume(0, 1)
self.assertEqual(v, v_expected)
exp_dna_cutoff_2 = 150
f_expected_2 = a + b / exp_dna_cutoff_2 + c / exp_dna_cutoff_2 ** 2
v_expected_2 = 100 * (f_expected_2 - f_expected)
v = s._getBeadsVolume(0, 2)
self.assertEqual(v, v_expected_2)
expected_dna_cutoff = 800
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 29 * f_expected
v = s._getBeadsVolume(1, 1)
self.assertEqual(v, v_expected)
exp_dna_cutoff_2 = 300
f_expected_2 = a + b / exp_dna_cutoff_2 + c / exp_dna_cutoff_2 ** 2
v_expected_2 = 29 * (f_expected_2 - f_expected)
v = s._getBeadsVolume(1, 2)
self.assertEqual(v, v_expected_2)
expected_dna_cutoff = 700
f_expected = a + b / expected_dna_cutoff + c / expected_dna_cutoff ** 2
v_expected = 50 * f_expected
v = s._getBeadsVolume(2, 1)
self.assertEqual(v, v_expected)
exp_dna_cutoff_2 = 100
f_expected_2 = a + b / exp_dna_cutoff_2 + c / exp_dna_cutoff_2 ** 2
v_expected_2 = 50 * (f_expected_2 - f_expected)
v = s._getBeadsVolume(2, 2)
self.assertEqual(v, v_expected_2)
def test_positionsToPurify2ndStage(self, mock_loadIndividualSettings):
param = 'Initial sample volume'
self.prep_settingsFile(param, [100, 29, 50, 0, 0, -1, -1, -1, -1, -1, -1, -1])
s = ponec.settings(self.mock_csv_path)
values = s._returnSampleParameterList(param)
self.assertEqual(values, [100, 29, 50])
