def helper(hashTable, reads, infoString):
counter, total, totalNum = 0, 0, 0
for readFile in reads:
if totalNum == kmerNum:
break
try:
readFastq, _ = getSeqfromRead(readFile)
except:
continue
hits = [
aln for aln in referenceIdx.map(readFastq)
if aln.q_en - aln.q_st > 0.95 * len(readFastq) and aln.strand == 1
]
if infoString == "+" and len(hits) != 1:
continue
if infoString == "-" and len(hits) != 0:
continue
readSignal = getSignalFromRead(readFile)
if len(readSignal) <= toRead:
continue
counter += 1
readSignal = readSignal[fromRead:toRead]
readString = getLevelStr(readSignal, levels)
for i in range(len(readString)):
if kmerNum == totalNum:
break
kmer = readString[i:i + k]
totalNum += 1
total += hashTable.get(kmer, 0)
print(f"{infoString} k {k} l {levels} -> {total} / {totalNum}")
readString = getLevelString(readSignal, smoothParam, levels, overflow)
readDict = buildDictionary(readString, kmerLength)
return overlap(readDict, hashTable)
########################################
data = []
for filePath in posFast5:
if posTestCases == 0:
break
try:
readSeq, basecallTable = getSeqfromRead(filePath)
except:
continue
if len(readSeq) < (toRead // repeatSignal):
continue
hits = [
aln for aln in referenceIdx.map(readSeq)
if aln.q_en - aln.q_st > 0.95 *
len(readSeq) and aln.strand == 1 and aln.ctg == workingContig
]
if len(hits) == 0:
continue
hit = hits[0]
posTestCases -= 1
lvlStringHits = processRead(filePath, workingContig)
### get corresponding part of the reference using minimap2
referenceIdx = mp.Aligner(refFile)
assert referenceIdx, "failed to load/build reference index"
mod = KmerModel.load_from_hdf5(kmerModelFilePath)
posReads = getReadsInFolder(readsPosFilePath, minSize=1000000)
negReads = getReadsInFolder(readsNegFilePath, minSize=1000000)
goodK, totalK = 0, 0
dobre, zle = 0, 0
for readFile in posReads[:min(len(posReads), maxTests)]:
#print(readFile)
try:
readFastq, readEvents = getSeqfromRead(readFile)
except:
continue
readSeq = seqSignalCor(signalFrom, signalTo, readEvents)
hits = [
aln for aln in referenceIdx.map(readSeq)
if aln.q_en - aln.q_st > 0.95 * len(readSeq)
]
if len(hits) != 1:
# print("Too many or too few hits, skipping read.")
continue
print(readFile)
hit = hits[0]
successfulReads += 1
levelStr = line[3]
storeContig[line[0]] = levelStr
hashTable = {}
hashTables = {}
for contigName in storeContig.keys():
hashTables[contigName] = {}
buildDictionarySpecial(hashTables[contigName], storeContig[contigName], kmerLen)
buildDictionarySpecial(hashTable, storeContig[contigName], kmerLen)
print("Preparation done!")
for sample in posReadsPaths[:400]:
try:
readFastq, readEvents = getSeqfromRead(sample)
except:
continue
hits = [
aln
for aln in referenceIdx.map(readFastq)
if aln.q_en - aln.q_st > 0.95 * len(readFastq)
and aln.strand == 1
and aln.ctg == workingContig
]
if len(hits) != 1:
continue
hit = hits[0]
refPosition = hit.r_st / len(ref[hit.ctg])
assert referenceIdx, "failed to load/build reference index"
posReads = getReadsInFolder(readsPosFilePath, minSize=0)
negReads = getReadsInFolder(readsNegFilePath, minSize=0)
################################################################################
negHitsByRatios = [0] * len(ratios)
totalCount = 0
for readFile in negReads:
if totalCount == readCount:
break
try:
readFastq, _ = getSeqfromRead(readFile)
except:
continue
hits = [aln for aln in referenceIdx.map(readFastq)]
hits = [(hit.q_en - hit.q_st) for hit in hits]
totalCount += 1
for i in reversed(range(len(ratios))):
hit = max(hits + [0])
if hit >= ratios[i] * len(readFastq):
negHitsByRatios[i] += 1
# break
posHitsByRatios = [0] * len(ratios)
################################################################################
pomery = [[[] for j in kmerLen] for i in levels]
overlap = [[[] for j in kmerLen] for i in levels]
goodDash = [[] for i in levels]
badDash = [[] for i in levels]
alignLenRead = [0 for _ in levels]
alignLenFake = [0 for _ in levels]
readCounter = 0
for posRead in posReads:
if readCounter == readNum:
break
try:
readFastq, readEvents = getSeqfromRead(posRead)
except:
continue
readSeq = seqSignalCor(signalFrom, signalTo, readEvents)
hits = [
aln for aln in referenceIdx.map(readSeq)
if aln.q_en - aln.q_st > 0.95 * len(readSeq)
]
if len(hits) != 1:
# print("Too many or too few hits, skipping read.")
continue
hit = hits[0]
print("Working on", posRead)
print(f"So far done {readCounter} reads")
import numpy as np
sys.path.append("../")
from signalHelper import getReadsInFolder, getSignalFromRead, getSeqfromRead
reads = getReadsInFolder(readsFilePath, minSize=0)
signalLengths, seqLengths = [], []
for read in reads:
if maxReads == 0:
break
try:
signal = getSignalFromRead(read)
seq, _ = getSeqfromRead(read)
except:
continue
maxReads -= 1
signalLengths.append(len(signal))
seqLengths.append(len(seq))
meanSignal = np.mean(signalLengths)
medianSignal = np.median(signalLengths)
meanSeq = np.mean(seqLengths)
medianSeq = np.median(seqLengths)
print(f"Not found {maxReads} reads!")
