def get_sum_of_rectangle(im_int, x, y, w, h):
x_f = round_i(x)
y_f = round_i(y)
x_t = round_i(x + w) - 1
y_t = round_i(y + h) - 1
area = integrate(im_int, y_f, x_f, y_t, x_t)
if isinstance(area, (collections.Sequence, np.ndarray)):
return area[0]
else:
return area
def extract_patch_on_slide(
file_path_tif, \
file_path_tis_mask, \
file_path_jpg, \
save_location_path_patch_position_visualize, \
save_location_path_patch_position_csv, \
size_patch):
"""
-- Intput :
file_path_tif : full path
file_path_tis_mask : full path
file_path_jpg : full path
save_location_path_patch_position_visualize : full path
save_location_path_patch_position_csv : full path
-- Result :
Draw patch position.
Save coordinate of patch at level_0.
"""
patch_level = 0
contours_level = 4
mask_level = 4
slide = OpenSlide(file_path_tif)
slide_w_lv_4, slide_h_lv_4 = slide.level_dimensions[4]
downsample = slide.level_downsamples[4]
size_patch_lv_4 = int(size_patch / downsample)
# Make integral image of slide
tissue_mask = cv2.imread(file_path_tis_mask, 0)
integral_image_tissue = integral_image(tissue_mask.T / 255)
# Load original bgr_jpg_lv_4 for visualizing patch position
wsi_bgr_jpg = cv2.imread(file_path_jpg)
wsi_jpg_visualizing_patch_position = wsi_bgr_jpg.copy()
print('==> making contours of tissue region from jpg ..')
# Find and Draw contours_tissue - (color : blue)
_, contours_tissue, _ = cv2.findContours( \
tissue_mask, \
cv2.RETR_TREE, \
cv2.CHAIN_APPROX_SIMPLE)
cv2.drawContours(wsi_jpg_visualizing_patch_position, \
contours_tissue, -1, (255, 0, 0), 2)
# Make csv_writer
csv_file = open(save_location_path_patch_position_csv, 'w')
fieldnames = ['X', 'Y']
csv_writer = csv.DictWriter(csv_file, fieldnames=fieldnames)
csv_writer.writeheader()
print('==> Extracting patches randomly on tissue region...')
patch_cnt = 0
### Extract random patches on tissue region
for contour in contours_tissue:
# Check if contour area is samller than patch area
area = cv2.contourArea(contour)
area_patch_lv_4 = size_patch_lv_4**2
if area < area_patch_lv_4:
continue
# Determine number of patches to extract
number_patches = int(round(area / area_patch_lv_4 * 1.5))
number_patches = min(50, number_patches)
print('contour area : ', area, ' num_patch : ', number_patches)
# Get coordinates of contour (level : 4)
coordinates = (np.squeeze(contour)).T
coords_x = coordinates[0]
coords_y = coordinates[1]
# Bounding box vertex
p_x_left = np.min(coords_x)
p_x_right = np.max(coords_x)
p_y_top = np.min(coords_y)
p_y_bottom = np.max(coords_y)
# Make candidates of patch coordinate (level : 4)
candidate_x = \
np.arange(round(p_x_left), round(p_x_right)).astype(int)
candidate_y = \
np.arange(round(p_y_top), round(p_y_bottom)).astype(int)
# Pick coordinates randomly
len_x = candidate_x.shape[0]
len_y = candidate_y.shape[0]
number_patches = min(number_patches, len_x)
number_patches = min(number_patches, len_y)
random_index_x = np.random.choice(len_x, number_patches, replace=False)
random_index_y = np.random.choice(len_y, number_patches, replace=True)
for i in range(number_patches):
patch_x = candidate_x[random_index_x[i]]
patch_y = candidate_y[random_index_y[i]]
# Check if out of range
if (patch_x + size_patch_lv_4 > slide_w_lv_4) or \
(patch_y + size_patch_lv_4 > slide_h_lv_4):
continue
# Check ratio of tumor region
tissue_integral = integrate(integral_image_tissue, \
(patch_x, patch_y), \
(patch_x + size_patch_lv_4 - 1,
patch_y + size_patch_lv_4 - 1))
tissue_ratio = tissue_integral / (size_patch_lv_4**2)
if tissue_ratio < 0.9:
continue
# Save patches position to csv file.
patch_x_lv_0 = int(round(patch_x * downsample))
patch_y_lv_0 = int(round(patch_y * downsample))
csv_writer.writerow({'X': patch_x_lv_0, 'Y': patch_y_lv_0})
patch_cnt += 1
# save cut patches
im = slide.read_region((patch_x_lv_0, patch_y_lv_0), 0,
(size_patch, size_patch))
im_rgba = np.array(im)
im_rgb = cv2.cvtColor(im_rgba, cv2.COLOR_RGBA2RGB)
cur_patient_name = file_path_tif.split('/')[4]
cur_patient_name = cur_patient_name.split('.')[0]
global ID
cur_cut_name = save_cut_path_positive_patch_17 + cur_patient_name + '_' + str(
ID) + '.jpg'
ID += 1
print('cur_cut_name', cur_cut_name)
cv2.imwrite(cur_cut_name, im_rgb)
# Draw patch position (color : Green)
cv2.rectangle(wsi_jpg_visualizing_patch_position, \
(patch_x, patch_y), \
(patch_x + size_patch_lv_4, patch_y + size_patch_lv_4), \
(0, 255, 0), \
thickness=1)
print('slide :\t', file_path_tif)
print('patch_cnt:\t', patch_cnt)
# Save visualizing image.
cv2.imwrite(save_location_path_patch_position_visualize, \
wsi_jpg_visualizing_patch_position)
csv_file.close()
def create_other_txt_sequence(file_path_tif,file_path_tis_mask,file_path_mask,list,\
file_path_txt,kind,patch_size,mask_downsample,stride):
txt = open(file_path_txt, 'a+')
for file in os.listdir(file_path_tif):
if "TCGA-2Y-A9H1" in file:
continue
file_name = file[:-4]
lab_mask_name = file_path_mask + file_name + '_mask_' + str(
mask_downsample) + '.png'
if (file_name in list) and (os.path.exists(lab_mask_name)):
tissue_mask_name = file_path_tis_mask + file_name + '_tissue_mask_' + str(
mask_downsample) + '.png'
tissue_mask = cv2.imread(tissue_mask_name, 0)
integral_image_tissue = integral_image(tissue_mask.T / 255)
# Make integral image of slide
lab_mask = cv2.imread(lab_mask_name, 0)
integral_image_lab = integral_image(lab_mask.T / 255)
size_patch_lv_k = int(patch_size /
mask_downsample) # patch在第mask_level层上映射的大小
print(size_patch_lv_k)
slide = OpenSlide(file_path_tif + file)
slide_w_lv_0, slide_h_lv_0 = slide.dimensions
slide_w = int(slide_w_lv_0 / mask_downsample)
slide_h = int(slide_h_lv_0 / mask_downsample)
_, contours_lab, _ = cv2.findContours(lab_mask, cv2.RETR_TREE,
cv2.CHAIN_APPROX_SIMPLE)
_, contours_tissue, _ = cv2.findContours(tissue_mask,
cv2.RETR_TREE,
cv2.CHAIN_APPROX_SIMPLE)
p_left = []
p_right = []
p_bottom = []
p_top = []
# Extract random patches on tissue region
for contour in contours_tissue:
coordinates = (np.squeeze(contour)).T
coords_x = coordinates[0]
coords_y = coordinates[1]
# Bounding box vertex
p_left.append(np.min(coords_x))
p_right.append(np.max(coords_x))
p_top.append(np.min(coords_y))
p_bottom.append(np.max(coords_y))
p_x_left = min(p_left)
p_x_right = max(p_right)
p_y_top = min(p_top)
p_y_bottom = max(p_bottom)
stride_lv = int(stride / mask_downsample)
print(p_x_left, p_x_right, p_y_top, p_y_bottom)
for x in range(p_x_left, p_x_right, stride_lv):
for y in range(p_y_top, p_y_bottom, stride_lv):
if (x + size_patch_lv_k - 1 >=
slide_w) or (y + size_patch_lv_k - 1 >= slide_h):
continue
tissue_integral = integrate(integral_image_tissue, \
(x, y), \
(x + size_patch_lv_k - 1,
y + size_patch_lv_k - 1)
)
tissue_ratio = tissue_integral / (size_patch_lv_k**2)
lab_integral = integrate(integral_image_lab, \
(x, y), \
(x + size_patch_lv_k - 1,
y + size_patch_lv_k - 1)
)
lab_ratio = lab_integral / (size_patch_lv_k**2)
if tissue_ratio > 0.8 and lab_ratio < 0.2:
x_lv = int(x + size_patch_lv_k / 2)
y_lv = int(y + size_patch_lv_k / 2)
patch_x_lv_0 = str((round(x_lv * mask_downsample)))
patch_y_lv_0 = str((round(y_lv * mask_downsample)))
txt.writelines([
file_name, ',', patch_x_lv_0, ',', patch_y_lv_0,
',', kind, '\n'
])
txt.close()
def patch_sampling(slide, mask, nonzerox, nonzeroy, **opts):
"""Patch sampling on whole slide image by random points over an uniform
distribution.
TO-DO
+++++
Optimize: compute non zero list in the caller and pass only the intersting
part -- will help in batch linear sampling...
Arguments
+++++++++
:param obj slide: OpenSlide Object
:param obj mask: mask image as (0, 1) int Numpy array
:param list nonzerox, nonzeroy: Numpy arrays of nonzero points over `mask`
Keyword arguments
+++++++++++++++++
:param int start_idx: start index on the mask's nonzero point
list. Ignored if `mode` == 'random'
:param obj logger: a pymod:logging instance
:param int slide_level: level of mask
:param int patch_size: size of patch scala integer n
:param int n_samples: the number of patches to extract (batch size)
...plaese complete me!
:return list:
patches (RGB images),
list of patch points (starting from left top),
last used nonzero mask point's index or None (random sampling only)
"""
global logger
# def values updated from **opts
dopts = {
'area_overlap' : .6,
'bad_batch_size' : 500,
'gray_threshold' : 90,
'margin_width_x' : 250, # as per original code, watch out!
'margin_width_y' : 50, # ditto
'method' : 'random',
'n_samples' : 100,
'patch_size' : 224,
'slide_level' : 5,
'start_idx' : 0,
'white_level' : 200,
'white_threshold' : .3,
'white_threshold_incr' : .05,
'white_threshold_max' : .7,
}
for dk in dopts:
# [BUG] if called with a missing key, won't get the default!?
try:
dopts[dk] = opts.pop(dk, None)
except KeyError as k:
pass
# reinject as standard var... This is just because I'm lazy and want
# to keep the original names ;-)
exec "{} = dopts[dk]".format(dk)
if opts:
# leftovers...
raise RuntimeError, 'Unexpected options {}'.format(opts)
logger.debug("kw opts:\n{}.".format(dopts))
# bind to aux functions
bfn = {
'get_index' : None,
'get_white_threshold' : None,
'is_batch_over' : None,
}
for n in bfn.keys():
bfn[n] = globals()['{}__{}'.format(n, method)]
if not callable(bfn[n]):
raise RuntimeError, '[BUG] {} => {}: invalid aux function binding'.format(n, bfn[n])
report = {
'out_of_boundary' : 0,
'on_border' : 0,
'black_patches' : 0,
'white_patches' : 0,
'gray_patches' : 0,
}
patch_list = []
patch_point = []
# patch size at given level or resolution
level_patch_size = int(patch_size / slide.level_downsamples[slide_level])
x_l, y_l = nonzerox, nonzeroy
x_ln, y_ln = len(x_l), len(y_l)
logger.info('Working mask has {} x {} nonzero points'.format(x_ln, len(y_l)))
if x_ln < level_patch_size * 2:
logger.info(
"Not enough nonzero mask points for at least 2 patches ({} < {})".format(
x_ln, level_patch_size
)
)
return [], [], None
# computing the actual level of resolution (dot product)
x_ws = (np.round(x_l * slide.level_downsamples[slide_level])).astype(int)
y_ws = (np.round(y_l * slide.level_downsamples[slide_level])).astype(int)
cnt = 0 # good patch counter
nt_cnt = 0 # not taken patch counter
p_iterator = bfn['get_index'](start_idx, x_ln - 1)
p_idx = start_idx # just for init purposes
# while(not bfn['is_batch_over'](cnt, n_samples)):
while(cnt < n_samples):
# pick an index...
try:
p_idx = p_iterator.next()
except StopIteration:
break
# ...corresponding point in the mask
level_point_x, level_point_y = x_l[p_idx], y_l[p_idx]
# [BUG] otsu threshold takes also border, so discard?? mmh, needs
# double check (risk missing stuff...)
if is_point_on_border(level_point_x, level_point_y, margin_width_x, margin_width_y):
# logger.debug(
# 'Skipping point on mask border: {}x ?< {}, {}y ?< {}'.format(
# level_point_x, margin_width_x, level_point_y, margin_width_y
# )
# )
report['on_border'] += 1
continue
if not is_point_within_boundaries(
level_point_x, level_point_y, level_patch_size, mask.shape
):
# logger.debug(
# 'Skipping point out of mask boundary: {} ?> {}, {} ?> {}'.format(
# level_point_x, mask.shape[0], level_point_y, mask.shape[1]
# )
# )
report['out_of_boundary'] += 1
continue
# make patch from mask image
level_patch_mask = mask[
int(level_point_x) : int(level_point_x + level_patch_size),
int(level_point_y) : int(level_point_y + level_patch_size)
]
# apply integral
ii_map = integral_image(level_patch_mask)
ii_sum = integrate(ii_map, (0, 0), (level_patch_size - 1, level_patch_size - 1))
# total patch area should covers at least x% of the annotation
# region
overlap = float(ii_sum) / (level_patch_size**2)
if overlap < area_overlap:
continue
# square patch (RGB point array in [0, 255])
patch = slide.read_region(
(y_ws[p_idx], x_ws[p_idx]), 0, (patch_size, patch_size)
)
patch = np.array(patch)
if np.sum(patch) == 0:
report['black_patches'] += 1
# logger.debug('Skipping black patch at {}, {}'.format(level_point_x, level_point_y))
continue
# check almost white RGB values.
white_mask = patch[:,:,0:3] > white_level
# sum over the 3 RGB channels
if float(np.sum(white_mask)) / (patch_size**2*3) <= white_threshold:
patch = cv2.cvtColor(patch, cv2.COLOR_RGBA2BGR)
if np.mean(patch) > gray_threshold:
# got a good one...
patch_list.append(patch)
# ...with its location
patch_point.append((x_l[p_idx], y_l[p_idx]))
cnt += 1
else:
report['gray_patches'] += 1
# logger.debug('Skipping grey patch at {}, {}'.format(x_l[p_idx], y_l[p_idx]))
else:
# bad one: too white
report['white_patches'] += 1
nt_cnt += 1
# possibly get an update
nt_cnt, white_threshold = bfn['get_white_threshold'](
nt_cnt, bad_batch_size, white_threshold, white_threshold_max, white_threshold_incr
)
if white_threshold == None:
logger.warning('Max white threshold reached! Bailing out')
break
# {end while}
logger.info(
'Skipped points: {} on mask boder, {} out of mask boundary'.format(
report['on_border'], report['out_of_boundary']
)
)
logger.info(
'Skipped patches: {} black, {} white, {} gray'.format(
report['black_patches'], report['white_patches'], report['gray_patches']
)
)
logger.info('Extracted {} patches'.format(len(patch_point)))
# in 'random' method, only one batch is done, so it doens't make sense to
# return the last index. Instead signal that we're over with sampling.
p_idx = None if method == 'random' else p_idx
return patch_list, patch_point, p_idx
def patch_sampling_using_integral(slide,slide_level,mask,patch_size,patch_num):
"""
patch sampling on whole slide image
input:
slide = OpenSlide Object
slide_level = level of mask
mask = mask image ( 0-1 int type nd-array)
patch_size = size of patch scala integer n
patch_num = the number of output patches
output:
list of patches(RGB Image), list of patch point (starting from left top)
"""
patch_list = [] # patches
patch_point = [] # patch locations
# taking the nonzero points in the mask
x_l,y_l = mask.nonzero()
#slide_level=7
if len(x_l) > patch_size/slide.level_downsamples[slide_level]*2:
level_patch_size = int(patch_size/slide.level_downsamples[slide_level])
print 'DEBUGGG: ', slide_level
# computing the actual level of resolution
# applying the nonzero mask as a dot product
x_ws = (np.round(x_l*slide.level_downsamples[slide_level])).astype(int)
y_ws = (np.round(y_l*slide.level_downsamples[slide_level])).astype(int)
cnt = 0 # patch counter
nt_cnt = 1 # not taken counter
white_threshold = .3
#white_threshold = 1.0
while(cnt < patch_num) :
# sampling from random distribution
p_idx = randint(0,len(x_l)-1)
# picking the random point in the mask
level_point_x,level_point_y = x_l[p_idx], y_l[p_idx]
if (level_point_y < 50) or (level_point_x < 250): ##new add to check
continue
# check the boundary to make patch
check_bound = np.resize(np.array([level_point_x+level_patch_size,level_point_y+level_patch_size]),(2,))
if check_bound[0] > mask.shape[0] or check_bound[1] > mask.shape[1]:
continue
# make patch from mask image
level_patch_mask = mask[int(level_point_x):int(level_point_x+level_patch_size),int(level_point_y):int(level_point_y+level_patch_size)]
# apply integral
ii_map = integral_image(level_patch_mask)
ii_sum = integrate(ii_map,(0,0),(level_patch_size-1,level_patch_size-1))
area_percent = float(ii_sum)/(level_patch_size**2)
# checking if the total area of the patch covers at least 80% of
# the annotation region
if area_percent<0.6:
continue
if cnt > patch_num*10+1000:
print "There is no more patches to extract in this slide"
print "mask region is too small"
print "final number of patches : ",len(patch_list)
break
patch=slide.read_region((y_ws[p_idx],x_ws[p_idx]),0,(patch_size,patch_size))
patch = np.array(patch)
#print '[integral] np.sum(patch): ', np.sum(patch)
if np.sum(patch)==0:
print('[integral] AaAaAH its zeroo!!')
continue
white_mask = patch[:,:,0:3] > 200
if float(np.sum(white_mask))/(patch_size**2*3) <= white_threshold :
#if True:
if np.sum(patch)>0:
# adding the patch to the patches list
patch_list.append(cv2.cvtColor(patch,cv2.COLOR_RGBA2BGR))
# adding the patch location to the list
patch_point.append((x_l[p_idx],y_l[p_idx]))
cnt += 1 # increasing patch counter
else:
print 'This is a black patch!'
else:
nt_cnt += 1
#print 'white_mask sum: ', np.sum(white_mask)
#print 'white ratio: ', float(np.sum(white_mask))/(patch_size**2*3)
#print 'Rejected location: {0},{1}'.format(x_l[p_idx],y_l[p_idx])
if nt_cnt %1000 == 0:
if white_threshold < .7:
white_threshold += .05
nt_cnt = 1
print 'Increasing white_threshold of 0.05: ', white_threshold
else:
print 'No more patches to extract that have more than 30 percent of not white content'
break
def_pl=[]
def_pp=[]
for i in range(len(patch_list)):
if (np.sum(patch_list[i])>0) and (np.mean(patch_list[i])>90):
def_pl.append(patch_list[i])
def_pp.append(patch_point[i])
return def_pl, def_pp
def tumor_patch_sampling_using_centerwin(slide,slide_level,mask,patch_size,patch_num):
"""
tumor patch sampling using center window
plz input the only tumor mask
it will malfunctioned if you input normal mask or tissue mask
input parameters are same as patch_sampling_using_integral
"""
patch_list = []
patch_point = []
window_size = int(32/ slide.level_downsamples[slide_level])
x_l,y_l = mask.nonzero()
if len(x_l) > patch_size*2:
level_patch_size = int(patch_size/slide.level_downsamples[slide_level])
x_ws = (np.round(x_l*slide.level_downsamples[slide_level])).astype(int)
y_ws = (np.round(y_l*slide.level_downsamples[slide_level])).astype(int)
cnt=0
while(len(patch_list) < patch_num) :
# loop cnt
cnt+=1
#random Pick point in mask
p_idx = randint(0,len(x_l)-1)
#Get the point in mask
level_point_x,level_point_y = x_l[p_idx], y_l[p_idx]
#Check boundary to make patch
check_bound = np.resize(np.array([level_point_x+level_patch_size,level_point_y+level_patch_size]),(2,))
if check_bound[0] > mask.shape[0] or check_bound[1] > mask.shape[1]:
continue
#make patch from mask image
level_patch_mask = mask[int(level_point_x):int(level_point_x+level_patch_size),int(level_point_y):int(level_point_y+level_patch_size)]
'''Biggest difference is here'''
#apply center window (32x32)
cntr_x= (level_patch_size/2)-1
cntr_y= (level_patch_size/2)-1
win_x = cntr_x-window_size/2
win_y = cntr_y-window_size/2
t_window = level_patch_mask[win_x:(win_x+window_size),win_y:(win_y+window_size)]
#print level_patch_mask.shape
#print win_x
#print win_y
#apply integral to window
ii_map = integral_image(t_window)
#print t_window.shape
ii_sum = integrate(ii_map,(0,0),(window_size-1,window_size-1))
area_percent = float(ii_sum)/(window_size**2)
# print "integral_area: ",area_percent
# print "loop count: ",cnt
if area_percent <1.0:
continue
if cnt > patch_num*10+1000:
print "There is no moare patches to extract in this slide"
print "mask region is too small"
print "final number of patches : ",len(patch_list)
break
#patch,point is appended the list
#print "region percent: ",area_percent
patch_point.append((x_l[p_idx],y_l[p_idx]))
patch=slide.read_region((y_ws[p_idx],x_ws[p_idx]),0,(patch_size,patch_size))
patch =np.array(patch)
patch_list.append(cv2.cvtColor(patch,cv2.COLOR_RGBA2BGR))
return patch_list, patch_point
def patch_sampling_using_integral(slide,slide_level,mask,patch_size,patch_num):
"""
patch sampling on whole slide image
slide = OpenSlide Object
slide_level = level of mask
mask = mask image ( 0-1 int type nd-array)
patch_size = size of patch scala integer n
patch_num = the number of output patches
return list of patches(RGB Image), list of patch point(left top)
"""
patch_list = []
patch_point = []
x_l,y_l = mask.nonzero()
if len(x_l) > patch_size*2:
level_patch_size = int(patch_size/slide.level_downsamples[slide_level])
x_ws = (np.round(x_l*slide.level_downsamples[slide_level])).astype(int)
y_ws = (np.round(y_l*slide.level_downsamples[slide_level])).astype(int)
cnt=0
while(len(patch_list) < patch_num) :
# loop cnt
cnt+=1
#random Pick point in mask
p_idx = randint(0,len(x_l)-1)
#Get the point in mask
level_point_x,level_point_y = x_l[p_idx], y_l[p_idx]
#Check boundary to make patch
check_bound = np.resize(np.array([level_point_x+level_patch_size,level_point_y+level_patch_size]),(2,))
if check_bound[0] > mask.shape[0] or check_bound[1] > mask.shape[1]:
continue
#make patch from mask image
level_patch_mask = mask[int(level_point_x):int(level_point_x+level_patch_size),int(level_point_y):int(level_point_y+level_patch_size)]
#apply integral
ii_map = integral_image(level_patch_mask)
ii_sum = integrate(ii_map,(0,0),(level_patch_size-1,level_patch_size-1))
area_percent = float(ii_sum)/(level_patch_size**2)
# print "integral_area: ",area_percent
# print "loop count: ",cnt
if area_percent<0.8:
continue
if cnt > patch_num*10+1000:
print "There is no moare patches to extract in this slide"
print "mask region is too small"
print "final number of patches : ",len(patch_list)
break
#patch,point is appended the list
#print "region percent: ",area_percent
patch_point.append((x_l[p_idx],y_l[p_idx]))
patch=slide.read_region((y_ws[p_idx],x_ws[p_idx]),0,(patch_size,patch_size))
patch =np.array(patch)
patch_list.append(cv2.cvtColor(patch,cv2.COLOR_RGBA2BGR))
return patch_list, patch_point
