def main(argv=None):
parser = get_parser()
if sys.platform.startswith("win32"):
# This isn't *really* a parsing error, but it feels a little more official to display the help with this error
parser.error(
"`sct_propseg` is not currently supported on native Windows installations. \n\n"
"For spinal cord segmentation, please migrate to the new and improved `sct_deepseg_sc` tool, "
"or consider using WSL to install SCT instead.\n\n"
"For further updates on `sct_propseg` Windows support, please visit:\n"
"https://github.com/spinalcordtoolbox/spinalcordtoolbox/issues/3694"
)
arguments = parser.parse_args(argv)
verbose = arguments.v
set_loglevel(verbose=verbose)
fname_input_data = os.path.abspath(arguments.i)
img_input = Image(fname_input_data)
img_seg = propseg(img_input, arguments)
fname_seg = img_seg.absolutepath
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
if path_qc is not None:
generate_qc(fname_in1=fname_input_data,
fname_seg=fname_seg,
args=arguments,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_propseg')
display_viewer_syntax([fname_input_data, fname_seg],
colormaps=['gray', 'red'],
opacities=['', '1'])
def gen_qc(path_qc):
t2_image = sct_test_path('t2', 't2.nii.gz')
t2_seg = sct_test_path('t2', 't2_seg-manual.nii.gz')
qc.generate_qc(fname_in1=t2_image,
fname_seg=t2_seg,
path_qc=path_qc,
process="sct_deepseg_gm")
def main(args=None):
if args is None:
args = sys.argv[1:]
# Get parser
parser = get_parser()
arguments = parser.parse(args)
# Set param arguments ad inputted by user
fname_in = arguments["-i"]
contrast = arguments["-c"]
# Segmentation or Centerline line
if '-s' in arguments:
fname_seg = arguments['-s']
if not os.path.isfile(fname_seg):
fname_seg = None
sct.printv('WARNING: -s input file: "' + arguments['-s'] + '" does not exist.\nDetecting PMJ without using segmentation information', 1, 'warning')
else:
fname_seg = None
# Output Folder
if '-ofolder' in arguments:
path_results = arguments["-ofolder"]
if not os.path.isdir(path_results) and os.path.exists(path_results):
sct.printv("ERROR output directory %s is not a valid directory" % path_results, 1, 'error')
if not os.path.exists(path_results):
os.makedirs(path_results)
else:
path_results = '.'
path_qc = arguments.get("-qc", None)
# Remove temp folder
rm_tmp = bool(int(arguments.get("-r", 1)))
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
# Initialize DetectPMJ
detector = DetectPMJ(fname_im=fname_in,
contrast=contrast,
fname_seg=fname_seg,
path_out=path_results,
verbose=verbose)
# run the extraction
fname_out, tmp_dir = detector.apply()
# Remove tmp_dir
if rm_tmp:
sct.rmtree(tmp_dir)
# View results
if fname_out is not None:
if path_qc is not None:
generate_qc(fname_in, fname_seg=fname_out, args=args, path_qc=os.path.abspath(path_qc),
process='sct_detect_pmj')
sct.display_viewer_syntax([fname_in, fname_out], colormaps=['gray', 'red'])
def main(args=None):
parser = get_parser()
arguments = parser.parse(sys.argv[1:])
fname_src = arguments["-d"]
fname_transfo = arguments["-w"]
warp_atlas = int(arguments["-a"])
warp_spinal_levels = int(arguments["-s"])
folder_out = arguments['-ofolder']
path_template = arguments['-t']
verbose = int(arguments['-v'])
path_qc = arguments.get("-qc", None)
# call main function
w = WarpTemplate(fname_src, fname_transfo, warp_atlas, warp_spinal_levels,
folder_out, path_template, verbose)
path_template = os.path.join(w.folder_out, w.folder_template)
# Only deal with QC and verbose if white matter was warped (meaning, everything under template/)
if "white matter" in spinalcordtoolbox.metadata.get_indiv_label_names(
path_template):
# Deal with QC report
if path_qc is not None:
fname_wm = os.path.join(
w.folder_out, w.folder_template,
spinalcordtoolbox.metadata.get_file_label(
path_template, 'white matter'))
generate_qc(fname_src,
fname_seg=fname_wm,
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
process='sct_warp_template')
# Deal with verbose
sct.display_viewer_syntax(
[
fname_src,
spinalcordtoolbox.metadata.get_file_label(
path_template,
'T2-weighted template',
output="filewithpath"),
spinalcordtoolbox.metadata.get_file_label(
path_template, 'gray matter', output="filewithpath"),
spinalcordtoolbox.metadata.get_file_label(
path_template, 'white matter', output="filewithpath")
],
colormaps=['gray', 'gray', 'red-yellow', 'blue-lightblue'],
opacities=['1', '1', '0.5', '0.5'],
minmax=['', '0,4000', '0.4,1', '0.4,1'],
verbose=verbose,
)
else:
if path_qc is not None:
sct.printv(
"QC not generated since expected labels are missing from template",
type="warning")
def main():
parser = get_parser()
arguments = parser.parse_args(args=None if sys.argv[1:] else ['--help'])
# Set param arguments ad inputted by user
fname_in = arguments.i
contrast = arguments.c
# Segmentation or Centerline line
if arguments.s is not None:
fname_seg = arguments.s
if not os.path.isfile(fname_seg):
fname_seg = None
printv('WARNING: -s input file: "' + arguments.s + '" does not exist.\nDetecting PMJ without using segmentation information', 1, 'warning')
else:
fname_seg = None
# Output Folder
if arguments.ofolder is not None:
path_results = arguments.ofolder
if not os.path.isdir(path_results) and os.path.exists(path_results):
printv("ERROR output directory %s is not a valid directory" % path_results, 1, 'error')
if not os.path.exists(path_results):
os.makedirs(path_results)
else:
path_results = '.'
path_qc = arguments.qc
# Remove temp folder
rm_tmp = bool(arguments.r)
verbose = arguments.v
init_sct(log_level=verbose, update=True) # Update log level
# Initialize DetectPMJ
detector = DetectPMJ(fname_im=fname_in,
contrast=contrast,
fname_seg=fname_seg,
path_out=path_results,
verbose=verbose)
# run the extraction
fname_out, tmp_dir = detector.apply()
# Remove tmp_dir
if rm_tmp:
rmtree(tmp_dir)
# View results
if fname_out is not None:
if path_qc is not None:
from spinalcordtoolbox.reports.qc import generate_qc
generate_qc(fname_in, fname_seg=fname_out, args=sys.argv[1:], path_qc=os.path.abspath(path_qc), process='sct_detect_pmj')
display_viewer_syntax([fname_in, fname_out], colormaps=['gray', 'red'])
def gen_qc(i):
t2_image = os.path.join(__sct_dir__, 'sct_testing_data', 't2', 't2.nii.gz')
t2_seg = os.path.join(__sct_dir__, 'sct_testing_data', 't2',
't2_seg.nii.gz')
qc.generate_qc(fname_in1=t2_image,
fname_seg=t2_seg,
path_qc="/tmp/qc",
process="sct_deepseg_gm")
return True
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv)
verbose = arguments.v
set_global_loglevel(verbose=verbose)
param = Param()
fname_src = arguments.d
fname_transfo = arguments.w
warp_atlas = arguments.a
warp_spinal_levels = arguments.s
folder_out = arguments.ofolder
path_template = arguments.t
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
folder_template = param.folder_template
folder_atlas = param.folder_atlas
folder_spinal_levels = param.folder_spinal_levels
file_info_label = param.file_info_label
list_labels_nn = param.list_labels_nn
# call main function
w = WarpTemplate(fname_src, fname_transfo, warp_atlas, warp_spinal_levels, folder_out, path_template,
folder_template, folder_atlas, folder_spinal_levels, file_info_label, list_labels_nn, verbose)
path_template = os.path.join(w.folder_out, w.folder_template)
# Deal with QC report
if path_qc is not None:
try:
fname_wm = os.path.join(
w.folder_out, w.folder_template, spinalcordtoolbox.metadata.get_file_label(path_template, id_label=4)) # label = 'white matter mask (probabilistic)'
generate_qc(
fname_src, fname_seg=fname_wm, args=sys.argv[1:], path_qc=os.path.abspath(path_qc), dataset=qc_dataset,
subject=qc_subject, process='sct_warp_template')
# If label is missing, get_file_label() throws a RuntimeError
except RuntimeError:
printv("QC not generated since expected labels are missing from template", type="warning")
# Deal with verbose
try:
display_viewer_syntax(
[fname_src,
spinalcordtoolbox.metadata.get_file_label(path_template, id_label=1, output="filewithpath"), # label = 'T2-weighted template'
spinalcordtoolbox.metadata.get_file_label(path_template, id_label=5, output="filewithpath"), # label = 'gray matter mask (probabilistic)'
spinalcordtoolbox.metadata.get_file_label(path_template, id_label=4, output="filewithpath")], # label = 'white matter mask (probabilistic)'
colormaps=['gray', 'gray', 'red-yellow', 'blue-lightblue'],
opacities=['1', '1', '0.5', '0.5'],
minmax=['', '0,4000', '0.4,1', '0.4,1'],
verbose=verbose)
# If label is missing, continue silently
except RuntimeError:
pass
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv)
verbose = arguments.v
set_global_loglevel(verbose=verbose)
# initialization
param = ParamMoco(is_diffusion=True, group_size=3, metric='MI', smooth='1')
# Fetch user arguments
param.fname_data = arguments.i
param.fname_bvecs = arguments.bvec
param.fname_bvals = arguments.bval
param.bval_min = arguments.bvalmin
param.group_size = arguments.g
param.fname_mask = arguments.m
param.interp = arguments.x
param.path_out = arguments.ofolder
param.remove_temp_files = arguments.r
if arguments.param is not None:
param.update(arguments.param)
path_qc = arguments.qc
qc_fps = arguments.qc_fps
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
qc_seg = arguments.qc_seg
mutually_inclusive_args = (path_qc, qc_seg)
is_qc_none, is_seg_none = [arg is None for arg in mutually_inclusive_args]
if not (is_qc_none == is_seg_none):
raise parser.error(
"Both '-qc' and '-qc-seg' are required in order to generate a QC report."
)
# run moco
fname_output_image = moco_wrapper(param)
set_global_loglevel(
verbose) # moco_wrapper changes verbose to 0, see issue #3341
# QC report
if path_qc is not None:
generate_qc(fname_in1=fname_output_image,
fname_in2=param.fname_data,
fname_seg=qc_seg,
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
fps=qc_fps,
dataset=qc_dataset,
subject=qc_subject,
process='sct_dmri_moco')
display_viewer_syntax([fname_output_image, param.fname_data],
mode='ortho,ortho')
def main(arguments):
fname_input_data = os.path.abspath(arguments["-i"])
img_input = Image(fname_input_data)
img_seg = propseg(img_input, arguments)
fname_seg = img_seg.absolutepath
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if path_qc is not None:
generate_qc(fname_in1=fname_input_data, fname_seg=fname_seg, args=args, path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_propseg')
sct.display_viewer_syntax([fname_input_data, fname_seg], colormaps=['gray', 'red'], opacities=['', '1'])
def gen_qc(args):
i, path_qc = args
t2_image = os.path.join(__sct_dir__, 'sct_testing_data', 't2', 't2.nii.gz')
t2_seg = os.path.join(__sct_dir__, 'sct_testing_data', 't2',
't2_seg-manual.nii.gz')
qc.generate_qc(fname_in1=t2_image,
fname_seg=t2_seg,
path_qc=path_qc,
process="sct_deepseg_gm")
return True
def main(arguments):
fname_input_data = os.path.abspath(arguments["-i"])
img_input = Image(fname_input_data)
img_seg = propseg(img_input, arguments)
fname_seg = img_seg.absolutepath
path_qc = arguments.get("-qc", None)
if path_qc is not None:
generate_qc(fname_in1=fname_input_data,
fname_seg=fname_seg,
args=args,
path_qc=os.path.abspath(path_qc),
process='sct_propseg')
sct.display_viewer_syntax([fname_input_data, fname_seg],
colormaps=['gray', 'red'],
opacities=['', '0.7'])
def main(args=None):
parser = get_parser()
arguments = parser.parse(sys.argv[1:])
fname_src = arguments["-d"]
fname_transfo = arguments["-w"]
warp_atlas = int(arguments["-a"])
warp_spinal_levels = int(arguments["-s"])
folder_out = arguments['-ofolder']
path_template = arguments['-t']
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
# call main function
w = WarpTemplate(fname_src, fname_transfo, warp_atlas, warp_spinal_levels, folder_out, path_template, verbose)
path_template = os.path.join(w.folder_out, w.folder_template)
# Only deal with QC and verbose if white matter was warped (meaning, everything under template/)
if "white matter" in spinalcordtoolbox.metadata.get_indiv_label_names(path_template):
# Deal with QC report
if path_qc is not None:
fname_wm = os.path.join(w.folder_out, w.folder_template,
spinalcordtoolbox.metadata.get_file_label(path_template, 'white matter'))
generate_qc(fname_src, fname_seg=fname_wm, args=sys.argv[1:], path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_warp_template')
# Deal with verbose
sct.display_viewer_syntax(
[
fname_src,
spinalcordtoolbox.metadata.get_file_label(path_template, 'T2-weighted template', output="filewithpath"),
spinalcordtoolbox.metadata.get_file_label(path_template, 'gray matter', output="filewithpath"),
spinalcordtoolbox.metadata.get_file_label(path_template, 'white matter', output="filewithpath")
],
colormaps=['gray', 'gray', 'red-yellow', 'blue-lightblue'],
opacities=['1', '1', '0.5', '0.5'],
minmax=['', '0,4000', '0.4,1', '0.4,1'],
verbose=verbose,
)
else:
if path_qc is not None:
sct.printv("QC not generated since expected labels are missing from template", type="warning" )
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv if argv else ['--help'])
verbose = arguments.v
set_global_loglevel(verbose=verbose)
input_filename = arguments.i
if arguments.o is not None:
output_filename = arguments.o
else:
output_filename = add_suffix(input_filename, '_gmseg')
use_tta = arguments.t
model_name = arguments.m
threshold = arguments.thr
if threshold > 1.0 or threshold < 0.0:
raise RuntimeError("Threshold should be between 0.0 and 1.0.")
# Threshold zero means no thresholding
if threshold == 0.0:
threshold = None
from spinalcordtoolbox.deepseg_gm import deepseg_gm
deepseg_gm.check_backend()
out_fname = deepseg_gm.segment_file(input_filename,
output_filename, model_name, threshold,
int(verbose), use_tta)
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
if path_qc is not None:
generate_qc(fname_in1=input_filename,
fname_seg=out_fname,
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_deepseg_gm')
display_viewer_syntax([input_filename, format(out_fname)],
colormaps=['gray', 'red'],
opacities=['1', '0.7'],
verbose=verbose)
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv)
verbose = arguments.v
set_global_loglevel(verbose=verbose)
fname_input_data = os.path.abspath(arguments.i)
img_input = Image(fname_input_data)
img_seg = propseg(img_input, arguments)
fname_seg = img_seg.absolutepath
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
if path_qc is not None:
generate_qc(fname_in1=fname_input_data, fname_seg=fname_seg, args=arguments, path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_propseg')
display_viewer_syntax([fname_input_data, fname_seg], colormaps=['gray', 'red'], opacities=['', '1'])
def main(args):
from spinalcordtoolbox.reports.qc import generate_qc
# Build args list (for display)
args_disp = '-i ' + args.i
if args.d:
args_disp += ' -d ' + args.d
if args.s:
args_disp += ' -s ' + args.s
generate_qc(fname_in1=args.i,
fname_in2=args.d,
fname_seg=args.s,
args=args_disp,
path_qc=args.qc,
dataset=args.qc_dataset,
subject=args.qc_subject,
process=args.p)
def main(args):
from spinalcordtoolbox.reports.qc import generate_qc
# Build args list (for display)
args_disp = '-i ' + args.i
if args.d:
args_disp += ' -d ' + args.d
if args.s:
args_disp += ' -s ' + args.s
generate_qc(fname_in1=args.i,
fname_in2=args.d,
fname_seg=args.s,
args=args_disp,
path_qc=args.qc,
dataset=args.qc_dataset,
subject=args.qc_subject,
process=args.p)
def main(arguments):
fname_input_data = os.path.abspath(arguments.i)
img_input = Image(fname_input_data)
img_seg = propseg(img_input, arguments)
fname_seg = img_seg.absolutepath
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
if path_qc is not None:
generate_qc(fname_in1=fname_input_data,
fname_seg=fname_seg,
args=arguments,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_propseg')
sct.display_viewer_syntax([fname_input_data, fname_seg],
colormaps=['gray', 'red'],
opacities=['', '1'])
def run_main():
parser = get_parser()
arguments = parser.parse(sys.argv[1:])
input_filename = arguments["-i"]
try:
output_filename = arguments["-o"]
except KeyError:
output_filename = sct.add_suffix(input_filename, '_gmseg')
use_tta = "-t" in arguments
verbose = arguments["-v"]
model_name = arguments["-m"]
threshold = arguments['-thr']
if threshold > 1.0 or threshold < 0.0:
raise RuntimeError("Threshold should be between 0.0 and 1.0.")
# Threshold zero means no thresholding
if threshold == 0.0:
threshold = None
from spinalcordtoolbox.deepseg_gm import deepseg_gm
deepseg_gm.check_backend()
out_fname = deepseg_gm.segment_file(input_filename,
output_filename, model_name, threshold,
int(verbose), use_tta)
path_qc = arguments.get("-qc", None)
if path_qc is not None:
generate_qc(fname_in1=input_filename,
fname_seg=out_fname,
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
process='sct_deepseg_gm')
sct.display_viewer_syntax(
[input_filename, format(out_fname)],
colormaps=['gray', 'red'],
opacities=['1', '0.7'],
verbose=verbose)
def run_main():
parser = get_parser()
arguments = parser.parse(sys.argv[1:])
input_filename = arguments["-i"]
try:
output_filename = arguments["-o"]
except KeyError:
output_filename = sct.add_suffix(input_filename, '_gmseg')
use_tta = "-t" in arguments
model_name = arguments["-m"]
threshold = arguments['-thr']
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
if threshold > 1.0 or threshold < 0.0:
raise RuntimeError("Threshold should be between 0.0 and 1.0.")
# Threshold zero means no thresholding
if threshold == 0.0:
threshold = None
from spinalcordtoolbox.deepseg_gm import deepseg_gm
deepseg_gm.check_backend()
out_fname = deepseg_gm.segment_file(input_filename, output_filename,
model_name, threshold, int(verbose),
use_tta)
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if path_qc is not None:
generate_qc(fname_in1=input_filename, fname_seg=out_fname, args=sys.argv[1:], path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_deepseg_gm')
sct.display_viewer_syntax([input_filename, format(out_fname)],
colormaps=['gray', 'red'],
opacities=['1', '0.7'],
verbose=verbose)
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv if argv else ['--help'])
verbose = arguments.v
set_global_loglevel(verbose=verbose)
from spinalcordtoolbox.reports.qc import generate_qc
# Build args list (for display)
args_disp = '-i ' + arguments.i
if arguments.d:
args_disp += ' -d ' + arguments.d
if arguments.s:
args_disp += ' -s ' + arguments.s
generate_qc(fname_in1=arguments.i,
fname_in2=arguments.d,
fname_seg=arguments.s,
args=args_disp,
path_qc=arguments.qc,
dataset=arguments.qc_dataset,
subject=arguments.qc_subject,
process=arguments.p)
def main(args=None):
if args is None:
args = sys.argv[1:]
# Get parser
parser = get_parser()
arguments = parser.parse(args)
# Set param arguments ad inputted by user
fname_in = arguments["-i"]
contrast = arguments["-c"]
# Segmentation or Centerline line
if '-s' in arguments:
fname_seg = arguments['-s']
if not os.path.isfile(fname_seg):
fname_seg = None
sct.printv(
'WARNING: -s input file: "' + arguments['-s'] +
'" does not exist.\nDetecting PMJ without using segmentation information',
1, 'warning')
else:
fname_seg = None
# Output Folder
if '-ofolder' in arguments:
path_results = arguments["-ofolder"]
if not os.path.isdir(path_results) and os.path.exists(path_results):
sct.printv(
"ERROR output directory %s is not a valid directory" %
path_results, 1, 'error')
if not os.path.exists(path_results):
os.makedirs(path_results)
else:
path_results = '.'
path_qc = arguments.get("-qc", None)
# Remove temp folder
rm_tmp = bool(int(arguments.get("-r", 1)))
# Verbosity
verbose = int(arguments.get("-v", 1))
# Initialize DetectPMJ
detector = DetectPMJ(fname_im=fname_in,
contrast=contrast,
fname_seg=fname_seg,
path_out=path_results,
verbose=verbose)
# run the extraction
fname_out, tmp_dir = detector.apply()
# Remove tmp_dir
if rm_tmp:
sct.rmtree(tmp_dir)
# View results
if fname_out is not None:
if path_qc is not None:
generate_qc(fname_in,
fname_seg=fname_out,
args=args,
path_qc=os.path.abspath(path_qc),
process='sct_detect_pmj')
sct.display_viewer_syntax([fname_in, fname_out],
colormaps=['gray', 'red'])
def main(argv=None):
parser = get_parser()
arguments = parser.parse_args(argv if argv else ['--help'])
verbose = arguments.v
set_global_loglevel(verbose=verbose)
# initializations
initz = ''
initcenter = ''
fname_initlabel = ''
file_labelz = 'labelz.nii.gz'
param = Param()
fname_in = os.path.abspath(arguments.i)
fname_seg = os.path.abspath(arguments.s)
contrast = arguments.c
path_template = os.path.abspath(arguments.t)
scale_dist = arguments.scale_dist
path_output = arguments.ofolder
param.path_qc = arguments.qc
if arguments.discfile is not None:
fname_disc = os.path.abspath(arguments.discfile)
else:
fname_disc = None
if arguments.initz is not None:
initz = arguments.initz
if len(initz) != 2:
raise ValueError(
'--initz takes two arguments: position in superior-inferior direction, label value'
)
if arguments.initcenter is not None:
initcenter = arguments.initcenter
# if user provided text file, parse and overwrite arguments
if arguments.initfile is not None:
file = open(arguments.initfile, 'r')
initfile = ' ' + file.read().replace('\n', '')
arg_initfile = initfile.split(' ')
for idx_arg, arg in enumerate(arg_initfile):
if arg == '-initz':
initz = [int(x) for x in arg_initfile[idx_arg + 1].split(',')]
if len(initz) != 2:
raise ValueError(
'--initz takes two arguments: position in superior-inferior direction, label value'
)
if arg == '-initcenter':
initcenter = int(arg_initfile[idx_arg + 1])
if arguments.initlabel is not None:
# get absolute path of label
fname_initlabel = os.path.abspath(arguments.initlabel)
if arguments.param is not None:
param.update(arguments.param[0])
remove_temp_files = arguments.r
clean_labels = arguments.clean_labels
laplacian = arguments.laplacian
path_tmp = tmp_create(basename="label_vertebrae")
# Copying input data to tmp folder
printv('\nCopying input data to tmp folder...', verbose)
Image(fname_in).save(os.path.join(path_tmp, "data.nii"))
Image(fname_seg).save(os.path.join(path_tmp, "segmentation.nii"))
# Go go temp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Straighten spinal cord
printv('\nStraighten spinal cord...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
cache_sig = cache_signature(input_files=[fname_in, fname_seg], )
cachefile = os.path.join(curdir, "straightening.cache")
if cache_valid(cachefile, cache_sig) and os.path.isfile(
os.path.join(
curdir, "warp_curve2straight.nii.gz")) and os.path.isfile(
os.path.join(
curdir,
"warp_straight2curve.nii.gz")) and os.path.isfile(
os.path.join(curdir, "straight_ref.nii.gz")):
# if they exist, copy them into current folder
printv('Reusing existing warping field which seems to be valid',
verbose, 'warning')
copy(os.path.join(curdir, "warp_curve2straight.nii.gz"),
'warp_curve2straight.nii.gz')
copy(os.path.join(curdir, "warp_straight2curve.nii.gz"),
'warp_straight2curve.nii.gz')
copy(os.path.join(curdir, "straight_ref.nii.gz"),
'straight_ref.nii.gz')
# apply straightening
s, o = run_proc([
'sct_apply_transfo', '-i', 'data.nii', '-w',
'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o',
'data_straight.nii'
])
else:
sct_straighten_spinalcord.main(argv=[
'-i',
'data.nii',
'-s',
'segmentation.nii',
'-r',
str(remove_temp_files),
'-v',
str(verbose),
])
cache_save(cachefile, cache_sig)
# resample to 0.5mm isotropic to match template resolution
printv('\nResample to 0.5mm isotropic...', verbose)
s, o = run_proc([
'sct_resample', '-i', 'data_straight.nii', '-mm', '0.5x0.5x0.5', '-x',
'linear', '-o', 'data_straightr.nii'
],
verbose=verbose)
# Apply straightening to segmentation
# N.B. Output is RPI
printv('\nApply straightening to segmentation...', verbose)
run_proc(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation.nii', 'data_straightr.nii',
'warp_curve2straight.nii.gz', 'segmentation_straight.nii', 'Linear'),
verbose=verbose,
is_sct_binary=True,
)
# Threshold segmentation at 0.5
run_proc([
'sct_maths', '-i', 'segmentation_straight.nii', '-thr', '0.5', '-o',
'segmentation_straight.nii'
], verbose)
# If disc label file is provided, label vertebrae using that file instead of automatically
if fname_disc:
# Apply straightening to disc-label
printv('\nApply straightening to disc labels...', verbose)
run_proc(
'sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' %
(fname_disc, 'data_straightr.nii', 'warp_curve2straight.nii.gz',
'labeldisc_straight.nii.gz', 'label'),
verbose=verbose)
label_vert('segmentation_straight.nii',
'labeldisc_straight.nii.gz',
verbose=1)
else:
# create label to identify disc
printv('\nCreate label to identify disc...', verbose)
fname_labelz = os.path.join(path_tmp, file_labelz)
if initz or initcenter:
if initcenter:
# find z centered in FOV
nii = Image('segmentation.nii').change_orientation("RPI")
nx, ny, nz, nt, px, py, pz, pt = nii.dim # Get dimensions
z_center = int(np.round(nz / 2)) # get z_center
initz = [z_center, initcenter]
im_label = create_labels_along_segmentation(
Image('segmentation.nii'), [(initz[0], initz[1])])
im_label.data = dilate(im_label.data, 3, 'ball')
im_label.save(fname_labelz)
elif fname_initlabel:
Image(fname_initlabel).save(fname_labelz)
else:
# automatically finds C2-C3 disc
im_data = Image('data.nii')
im_seg = Image('segmentation.nii')
if not remove_temp_files: # because verbose is here also used for keeping temp files
verbose_detect_c2c3 = 2
else:
verbose_detect_c2c3 = 0
im_label_c2c3 = detect_c2c3(im_data,
im_seg,
contrast,
verbose=verbose_detect_c2c3)
ind_label = np.where(im_label_c2c3.data)
if not np.size(ind_label) == 0:
im_label_c2c3.data[ind_label] = 3
else:
printv(
'Automatic C2-C3 detection failed. Please provide manual label with sct_label_utils',
1, 'error')
sys.exit()
im_label_c2c3.save(fname_labelz)
# dilate label so it is not lost when applying warping
dilate(Image(fname_labelz), 3, 'ball').save(fname_labelz)
# Apply straightening to z-label
printv('\nAnd apply straightening to label...', verbose)
run_proc(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
(file_labelz, 'data_straightr.nii', 'warp_curve2straight.nii.gz',
'labelz_straight.nii.gz', 'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
# get z value and disk value to initialize labeling
printv('\nGet z and disc values from straight label...', verbose)
init_disc = get_z_and_disc_values_from_label('labelz_straight.nii.gz')
printv('.. ' + str(init_disc), verbose)
# apply laplacian filtering
if laplacian:
printv('\nApply Laplacian filter...', verbose)
run_proc([
'sct_maths', '-i', 'data_straightr.nii', '-laplacian', '1',
'-o', 'data_straightr.nii'
], verbose)
# detect vertebral levels on straight spinal cord
init_disc[1] = init_disc[1] - 1
vertebral_detection('data_straightr.nii',
'segmentation_straight.nii',
contrast,
param,
init_disc=init_disc,
verbose=verbose,
path_template=path_template,
path_output=path_output,
scale_dist=scale_dist)
# un-straighten labeled spinal cord
printv('\nUn-straighten labeling...', verbose)
run_proc(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation_straight_labeled.nii', 'segmentation.nii',
'warp_straight2curve.nii.gz', 'segmentation_labeled.nii',
'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
if clean_labels:
# Clean labeled segmentation
printv(
'\nClean labeled segmentation (correct interpolation errors)...',
verbose)
clean_labeled_segmentation('segmentation_labeled.nii',
'segmentation.nii',
'segmentation_labeled.nii')
# label discs
printv('\nLabel discs...', verbose)
printv('\nUn-straighten labeled discs...', verbose)
run_proc(
'sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' %
('segmentation_straight_labeled_disc.nii', 'segmentation.nii',
'warp_straight2curve.nii.gz', 'segmentation_labeled_disc.nii',
'label'),
verbose=verbose,
is_sct_binary=True,
)
# come back
os.chdir(curdir)
# Generate output files
path_seg, file_seg, ext_seg = extract_fname(fname_seg)
fname_seg_labeled = os.path.join(path_output,
file_seg + '_labeled' + ext_seg)
printv('\nGenerate output files...', verbose)
generate_output_file(os.path.join(path_tmp, "segmentation_labeled.nii"),
fname_seg_labeled)
generate_output_file(
os.path.join(path_tmp, "segmentation_labeled_disc.nii"),
os.path.join(path_output, file_seg + '_labeled_discs' + ext_seg))
# copy straightening files in case subsequent SCT functions need them
generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"),
os.path.join(path_output,
"warp_curve2straight.nii.gz"),
verbose=verbose)
generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"),
os.path.join(path_output,
"warp_straight2curve.nii.gz"),
verbose=verbose)
generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"),
os.path.join(path_output, "straight_ref.nii.gz"),
verbose=verbose)
# Remove temporary files
if remove_temp_files == 1:
printv('\nRemove temporary files...', verbose)
rmtree(path_tmp)
# Generate QC report
if param.path_qc is not None:
path_qc = os.path.abspath(arguments.qc)
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
labeled_seg_file = os.path.join(path_output,
file_seg + '_labeled' + ext_seg)
generate_qc(fname_in,
fname_seg=labeled_seg_file,
args=argv,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_label_vertebrae')
display_viewer_syntax([fname_in, fname_seg_labeled],
colormaps=['', 'subcortical'],
opacities=['1', '0.5'])
def main(argv: Sequence[str]):
"""
Main function. When this script is run via CLI, the main function is called using main(sys.argv[1:]).
:param argv: A list of unparsed arguments, which is passed to ArgumentParser.parse_args()
"""
for i, arg in enumerate(argv):
if arg == '-create-seg' and len(argv) > i+1 and '-1,' in argv[i+1]:
raise DeprecationWarning("The use of '-1' for '-create-seg' has been deprecated. Please use "
"'-create-seg-mid' instead.")
parser = get_parser()
arguments = parser.parse_args(argv)
verbose = arguments.v
set_global_loglevel(verbose=verbose)
input_filename = arguments.i
output_fname = arguments.o
img = Image(input_filename)
dtype = None
if arguments.add is not None:
value = arguments.add
out = sct_labels.add(img, value)
elif arguments.create is not None:
labels = arguments.create
out = sct_labels.create_labels_empty(img, labels)
elif arguments.create_add is not None:
labels = arguments.create_add
out = sct_labels.create_labels(img, labels)
elif arguments.create_seg is not None:
labels = arguments.create_seg
out = sct_labels.create_labels_along_segmentation(img, labels)
elif arguments.create_seg_mid is not None:
labels = [(-1, arguments.create_seg_mid)]
out = sct_labels.create_labels_along_segmentation(img, labels)
elif arguments.cubic_to_point:
out = sct_labels.cubic_to_point(img)
elif arguments.display:
display_voxel(img, verbose)
return
elif arguments.increment:
out = sct_labels.increment_z_inverse(img)
elif arguments.disc is not None:
ref = Image(arguments.disc)
out = sct_labels.labelize_from_discs(img, ref)
elif arguments.vert_body is not None:
levels = arguments.vert_body
if len(levels) == 1 and levels[0] == 0:
levels = None # all levels
out = sct_labels.label_vertebrae(img, levels)
elif arguments.vert_continuous:
out = sct_labels.continuous_vertebral_levels(img)
dtype = 'float32'
elif arguments.MSE is not None:
ref = Image(arguments.MSE)
mse = sct_labels.compute_mean_squared_error(img, ref)
printv(f"Computed MSE: {mse}")
return
elif arguments.remove_reference is not None:
ref = Image(arguments.remove_reference)
out = sct_labels.remove_missing_labels(img, ref)
elif arguments.remove_sym is not None:
# first pass use img as source
ref = Image(arguments.remove_reference)
out = sct_labels.remove_missing_labels(img, ref)
# second pass use previous pass result as reference
ref_out = sct_labels.remove_missing_labels(ref, out)
ref_out.save(path=ref.absolutepath)
elif arguments.remove is not None:
labels = arguments.remove
out = sct_labels.remove_labels_from_image(img, labels)
elif arguments.keep is not None:
labels = arguments.keep
out = sct_labels.remove_other_labels_from_image(img, labels)
elif arguments.create_viewer is not None:
msg = "" if arguments.msg is None else f"{arguments.msg}\n"
if arguments.ilabel is not None:
input_labels_img = Image(arguments.ilabel)
out = launch_manual_label_gui(img, input_labels_img, parse_num_list(arguments.create_viewer), msg)
else:
out = launch_sagittal_viewer(img, parse_num_list(arguments.create_viewer), msg)
printv("Generating output files...")
out.save(path=output_fname, dtype=dtype)
display_viewer_syntax([input_filename, output_fname])
if arguments.qc is not None:
generate_qc(fname_in1=input_filename, fname_seg=output_fname, args=argv,
path_qc=os.path.abspath(arguments.qc), dataset=arguments.qc_dataset,
subject=arguments.qc_subject, process='sct_label_utils')
def main(args=None):
parser = get_parser()
if args:
arguments = parser.parse_args(args)
else:
arguments = parser.parse_args(
args=None if sys.argv[1:] else ['--help'])
# Initialization
slices = ''
group_funcs = (('MEAN', func_wa), ('STD', func_std)
) # functions to perform when aggregating metrics along S-I
fname_segmentation = get_absolute_path(arguments.i)
fname_vert_levels = ''
if arguments.o is not None:
file_out = os.path.abspath(arguments.o)
else:
file_out = ''
if arguments.append is not None:
append = arguments.append
else:
append = 0
if arguments.vert is not None:
vert_levels = arguments.vert
else:
vert_levels = ''
remove_temp_files = arguments.r
if arguments.vertfile is not None:
fname_vert_levels = arguments.vertfile
if arguments.perlevel is not None:
perlevel = arguments.perlevel
else:
perlevel = None
if arguments.z is not None:
slices = arguments.z
if arguments.perslice is not None:
perslice = arguments.perslice
else:
perslice = None
angle_correction = arguments.angle_corr
param_centerline = ParamCenterline(algo_fitting=arguments.centerline_algo,
smooth=arguments.centerline_smooth,
minmax=True)
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
verbose = int(arguments.v)
init_sct(log_level=verbose, update=True) # Update log level
# update fields
metrics_agg = {}
if not file_out:
file_out = 'csa.csv'
metrics, fit_results = compute_shape(fname_segmentation,
angle_correction=angle_correction,
param_centerline=param_centerline,
verbose=verbose)
for key in metrics:
if key == 'length':
# For computing cord length, slice-wise length needs to be summed across slices
metrics_agg[key] = aggregate_per_slice_or_level(
metrics[key],
slices=parse_num_list(slices),
levels=parse_num_list(vert_levels),
perslice=perslice,
perlevel=perlevel,
vert_level=fname_vert_levels,
group_funcs=(('SUM', func_sum), ))
else:
# For other metrics, we compute the average and standard deviation across slices
metrics_agg[key] = aggregate_per_slice_or_level(
metrics[key],
slices=parse_num_list(slices),
levels=parse_num_list(vert_levels),
perslice=perslice,
perlevel=perlevel,
vert_level=fname_vert_levels,
group_funcs=group_funcs)
metrics_agg_merged = merge_dict(metrics_agg)
save_as_csv(metrics_agg_merged,
file_out,
fname_in=fname_segmentation,
append=append)
# QC report (only show CSA for clarity)
if path_qc is not None:
generate_qc(fname_segmentation,
args=args,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
path_img=_make_figure(metrics_agg_merged, fit_results),
process='sct_process_segmentation')
display_open(file_out)
def main(args=None):
# initializations
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_data = arguments['-i']
fname_seg = arguments['-s']
if '-l' in arguments:
fname_landmarks = arguments['-l']
label_type = 'body'
elif '-ldisc' in arguments:
fname_landmarks = arguments['-ldisc']
label_type = 'disc'
else:
sct.printv('ERROR: Labels should be provided.', 1, 'error')
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = ''
param.path_qc = arguments.get("-qc", None)
path_template = arguments['-t']
contrast_template = arguments['-c']
ref = arguments['-ref']
param.remove_temp_files = int(arguments.get('-r'))
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
param.verbose = verbose # TODO: not clean, unify verbose or param.verbose in code, but not both
param_centerline = ParamCenterline(
algo_fitting=arguments['-centerline-algo'],
smooth=arguments['-centerline-smooth'])
# registration parameters
if '-param' in arguments:
# reset parameters but keep step=0 (might be overwritten if user specified step=0)
paramreg = ParamregMultiStep([step0])
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# add user parameters
for paramStep in arguments['-param']:
paramreg.addStep(paramStep)
else:
paramreg = ParamregMultiStep([step0, step1, step2])
# if ref=subject, initialize registration using different affine parameters
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# initialize other parameters
zsubsample = param.zsubsample
# retrieve template file names
file_template_vertebral_labeling = get_file_label(os.path.join(path_template, 'template'), 'vertebral labeling')
file_template = get_file_label(os.path.join(path_template, 'template'), contrast_template.upper() + '-weighted template')
file_template_seg = get_file_label(os.path.join(path_template, 'template'), 'spinal cord')
# start timer
start_time = time.time()
# get fname of the template + template objects
fname_template = os.path.join(path_template, 'template', file_template)
fname_template_vertebral_labeling = os.path.join(path_template, 'template', file_template_vertebral_labeling)
fname_template_seg = os.path.join(path_template, 'template', file_template_seg)
fname_template_disc_labeling = os.path.join(path_template, 'template', 'PAM50_label_disc.nii.gz')
# check file existence
# TODO: no need to do that!
sct.printv('\nCheck template files...')
sct.check_file_exist(fname_template, verbose)
sct.check_file_exist(fname_template_vertebral_labeling, verbose)
sct.check_file_exist(fname_template_seg, verbose)
path_data, file_data, ext_data = sct.extract_fname(fname_data)
# sct.printv(arguments)
sct.printv('\nCheck parameters:', verbose)
sct.printv(' Data: ' + fname_data, verbose)
sct.printv(' Landmarks: ' + fname_landmarks, verbose)
sct.printv(' Segmentation: ' + fname_seg, verbose)
sct.printv(' Path template: ' + path_template, verbose)
sct.printv(' Remove temp files: ' + str(param.remove_temp_files), verbose)
# check input labels
labels = check_labels(fname_landmarks, label_type=label_type)
vertebral_alignment = False
if len(labels) > 2 and label_type == 'disc':
vertebral_alignment = True
path_tmp = sct.tmp_create(basename="register_to_template", verbose=verbose)
# set temporary file names
ftmp_data = 'data.nii'
ftmp_seg = 'seg.nii.gz'
ftmp_label = 'label.nii.gz'
ftmp_template = 'template.nii'
ftmp_template_seg = 'template_seg.nii.gz'
ftmp_template_label = 'template_label.nii.gz'
# copy files to temporary folder
sct.printv('\nCopying input data to tmp folder and convert to nii...', verbose)
Image(fname_data).save(os.path.join(path_tmp, ftmp_data))
Image(fname_seg).save(os.path.join(path_tmp, ftmp_seg))
Image(fname_landmarks).save(os.path.join(path_tmp, ftmp_label))
Image(fname_template).save(os.path.join(path_tmp, ftmp_template))
Image(fname_template_seg).save(os.path.join(path_tmp, ftmp_template_seg))
Image(fname_template_vertebral_labeling).save(os.path.join(path_tmp, ftmp_template_label))
if label_type == 'disc':
Image(fname_template_disc_labeling).save(os.path.join(path_tmp, ftmp_template_label))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Generate labels from template vertebral labeling
if label_type == 'body':
sct.printv('\nGenerate labels from template vertebral labeling', verbose)
ftmp_template_label_, ftmp_template_label = ftmp_template_label, sct.add_suffix(ftmp_template_label, "_body")
sct_label_utils.main(args=['-i', ftmp_template_label_, '-vert-body', '0', '-o', ftmp_template_label])
# check if provided labels are available in the template
sct.printv('\nCheck if provided labels are available in the template', verbose)
image_label_template = Image(ftmp_template_label)
labels_template = image_label_template.getNonZeroCoordinates(sorting='value')
if labels[-1].value > labels_template[-1].value:
sct.printv('ERROR: Wrong landmarks input. Labels must have correspondence in template space. \nLabel max '
'provided: ' + str(labels[-1].value) + '\nLabel max from template: ' +
str(labels_template[-1].value), verbose, 'error')
# if only one label is present, force affine transformation to be Tx,Ty,Tz only (no scaling)
if len(labels) == 1:
paramreg.steps['0'].dof = 'Tx_Ty_Tz'
sct.printv('WARNING: Only one label is present. Forcing initial transformation to: ' + paramreg.steps['0'].dof,
1, 'warning')
# Project labels onto the spinal cord centerline because later, an affine transformation is estimated between the
# template's labels (centered in the cord) and the subject's labels (assumed to be centered in the cord).
# If labels are not centered, mis-registration errors are observed (see issue #1826)
ftmp_label = project_labels_on_spinalcord(ftmp_label, ftmp_seg, param_centerline)
# binarize segmentation (in case it has values below 0 caused by manual editing)
sct.printv('\nBinarize segmentation', verbose)
ftmp_seg_, ftmp_seg = ftmp_seg, sct.add_suffix(ftmp_seg, "_bin")
sct_maths.main(['-i', ftmp_seg_,
'-bin', '0.5',
'-o', ftmp_seg])
# Switch between modes: subject->template or template->subject
if ref == 'template':
# resample data to 1mm isotropic
sct.printv('\nResample data to 1mm isotropic...', verbose)
resample_file(ftmp_data, add_suffix(ftmp_data, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_data = add_suffix(ftmp_data, '_1mm')
resample_file(ftmp_seg, add_suffix(ftmp_seg, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_seg = add_suffix(ftmp_seg, '_1mm')
# N.B. resampling of labels is more complicated, because they are single-point labels, therefore resampling
# with nearest neighbour can make them disappear.
resample_labels(ftmp_label, ftmp_data, add_suffix(ftmp_label, '_1mm'))
ftmp_label = add_suffix(ftmp_label, '_1mm')
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
if vertebral_alignment:
# cropping the segmentation based on the label coverage to ensure good registration with vertebral alignment
# See https://github.com/neuropoly/spinalcordtoolbox/pull/1669 for details
image_labels = Image(ftmp_label)
coordinates_labels = image_labels.getNonZeroCoordinates(sorting='z')
nx, ny, nz, nt, px, py, pz, pt = image_labels.dim
offset_crop = 10.0 * pz # cropping the image 10 mm above and below the highest and lowest label
cropping_slices = [coordinates_labels[0].z - offset_crop, coordinates_labels[-1].z + offset_crop]
# make sure that the cropping slices do not extend outside of the slice range (issue #1811)
if cropping_slices[0] < 0:
cropping_slices[0] = 0
if cropping_slices[1] > nz:
cropping_slices[1] = nz
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, np.int32(np.round(cropping_slices))),))).save(ftmp_seg)
else:
# if we do not align the vertebral levels, we crop the segmentation from top to bottom
im_seg_rpi = Image(ftmp_seg_)
bottom = 0
for data in msct_image.SlicerOneAxis(im_seg_rpi, "IS"):
if (data != 0).any():
break
bottom += 1
top = im_seg_rpi.data.shape[2]
for data in msct_image.SlicerOneAxis(im_seg_rpi, "SI"):
if (data != 0).any():
break
top -= 1
msct_image.spatial_crop(im_seg_rpi, dict(((2, (bottom, top)),))).save(ftmp_seg)
# straighten segmentation
sct.printv('\nStraighten the spinal cord using centerline/segmentation...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
fn_warp_curve2straight = os.path.join(curdir, "warp_curve2straight.nii.gz")
fn_warp_straight2curve = os.path.join(curdir, "warp_straight2curve.nii.gz")
fn_straight_ref = os.path.join(curdir, "straight_ref.nii.gz")
cache_input_files=[ftmp_seg]
if vertebral_alignment:
cache_input_files += [
ftmp_template_seg,
ftmp_label,
ftmp_template_label,
]
cache_sig = sct.cache_signature(
input_files=cache_input_files,
)
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(fn_warp_curve2straight) and os.path.isfile(fn_warp_straight2curve) and os.path.isfile(fn_straight_ref):
sct.printv('Reusing existing warping field which seems to be valid', verbose, 'warning')
sct.copy(fn_warp_curve2straight, 'warp_curve2straight.nii.gz')
sct.copy(fn_warp_straight2curve, 'warp_straight2curve.nii.gz')
sct.copy(fn_straight_ref, 'straight_ref.nii.gz')
# apply straightening
sct_apply_transfo.main(args=[
'-i', ftmp_seg,
'-w', 'warp_curve2straight.nii.gz',
'-d', 'straight_ref.nii.gz',
'-o', add_suffix(ftmp_seg, '_straight')])
else:
from spinalcordtoolbox.straightening import SpinalCordStraightener
sc_straight = SpinalCordStraightener(ftmp_seg, ftmp_seg)
sc_straight.param_centerline = param_centerline
sc_straight.output_filename = add_suffix(ftmp_seg, '_straight')
sc_straight.path_output = './'
sc_straight.qc = '0'
sc_straight.remove_temp_files = param.remove_temp_files
sc_straight.verbose = verbose
if vertebral_alignment:
sc_straight.centerline_reference_filename = ftmp_template_seg
sc_straight.use_straight_reference = True
sc_straight.discs_input_filename = ftmp_label
sc_straight.discs_ref_filename = ftmp_template_label
sc_straight.straighten()
sct.cache_save(cachefile, cache_sig)
# N.B. DO NOT UPDATE VARIABLE ftmp_seg BECAUSE TEMPORARY USED LATER
# re-define warping field using non-cropped space (to avoid issue #367)
sct_concat_transfo.main(args=[
'-w', 'warp_straight2curve.nii.gz',
'-d', ftmp_data,
'-o', 'warp_straight2curve.nii.gz'])
if vertebral_alignment:
sct.copy('warp_curve2straight.nii.gz', 'warp_curve2straightAffine.nii.gz')
else:
# Label preparation:
# --------------------------------------------------------------------------------
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Dilating the input label so they can be straighten without losing them
sct.printv('\nDilating input labels using 3vox ball radius')
sct_maths.main(['-i', ftmp_label,
'-dilate', '3',
'-o', add_suffix(ftmp_label, '_dilate')])
ftmp_label = add_suffix(ftmp_label, '_dilate')
# Apply straightening to labels
sct.printv('\nApply straightening to labels...', verbose)
sct_apply_transfo.main(args=[
'-i', ftmp_label,
'-o', add_suffix(ftmp_label, '_straight'),
'-d', add_suffix(ftmp_seg, '_straight'),
'-w', 'warp_curve2straight.nii.gz',
'-x', 'nn'])
ftmp_label = add_suffix(ftmp_label, '_straight')
# Compute rigid transformation straight landmarks --> template landmarks
sct.printv('\nEstimate transformation for step #0...', verbose)
try:
register_landmarks(ftmp_label, ftmp_template_label, paramreg.steps['0'].dof,
fname_affine='straight2templateAffine.txt', verbose=verbose)
except RuntimeError:
raise('Input labels do not seem to be at the right place. Please check the position of the labels. '
'See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42')
# Concatenate transformations: curve --> straight --> affine
sct.printv('\nConcatenate transformations: curve --> straight --> affine...', verbose)
sct_concat_transfo.main(args=[
'-w', ['warp_curve2straight.nii.gz', 'straight2templateAffine.txt'],
'-d', 'template.nii',
'-o', 'warp_curve2straightAffine.nii.gz'])
# Apply transformation
sct.printv('\nApply transformation...', verbose)
sct_apply_transfo.main(args=[
'-i', ftmp_data,
'-o', add_suffix(ftmp_data, '_straightAffine'),
'-d', ftmp_template,
'-w', 'warp_curve2straightAffine.nii.gz'])
ftmp_data = add_suffix(ftmp_data, '_straightAffine')
sct_apply_transfo.main(args=[
'-i', ftmp_seg,
'-o', add_suffix(ftmp_seg, '_straightAffine'),
'-d', ftmp_template,
'-w', 'warp_curve2straightAffine.nii.gz',
'-x', 'linear'])
ftmp_seg = add_suffix(ftmp_seg, '_straightAffine')
"""
# Benjamin: Issue from Allan Martin, about the z=0 slice that is screwed up, caused by the affine transform.
# Solution found: remove slices below and above landmarks to avoid rotation effects
points_straight = []
for coord in landmark_template:
points_straight.append(coord.z)
min_point, max_point = int(np.round(np.min(points_straight))), int(np.round(np.max(points_straight)))
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_black')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (min_point,max_point)),))).save(ftmp_seg)
"""
# open segmentation
im = Image(ftmp_seg)
im_new = msct_image.empty_like(im)
# binarize
im_new.data = im.data > 0.5
# find min-max of anat2template (for subsequent cropping)
zmin_template, zmax_template = msct_image.find_zmin_zmax(im_new, threshold=0.5)
# save binarized segmentation
im_new.save(add_suffix(ftmp_seg, '_bin')) # unused?
# crop template in z-direction (for faster processing)
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nCrop data in template space (for faster processing)...', verbose)
ftmp_template_, ftmp_template = ftmp_template, add_suffix(ftmp_template, '_crop')
msct_image.spatial_crop(Image(ftmp_template_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template)
ftmp_template_seg_, ftmp_template_seg = ftmp_template_seg, add_suffix(ftmp_template_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_template_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template_seg)
ftmp_data_, ftmp_data = ftmp_data, add_suffix(ftmp_data, '_crop')
msct_image.spatial_crop(Image(ftmp_data_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_data)
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_seg)
# sub-sample in z-direction
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nSub-sample in z-direction (for faster processing)...', verbose)
sct.run(['sct_resample', '-i', ftmp_template, '-o', add_suffix(ftmp_template, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template = add_suffix(ftmp_template, '_sub')
sct.run(['sct_resample', '-i', ftmp_template_seg, '-o', add_suffix(ftmp_template_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template_seg = add_suffix(ftmp_template_seg, '_sub')
sct.run(['sct_resample', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_data = add_suffix(ftmp_data, '_sub')
sct.run(['sct_resample', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_seg = add_suffix(ftmp_seg, '_sub')
# Registration straight spinal cord to template
sct.printv('\nRegister straight spinal cord to template...', verbose)
# loop across registration steps
warp_forward = []
warp_inverse = []
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_data
dest = ftmp_template
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_seg
dest = ftmp_template_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog':
src_seg = ftmp_seg
dest_seg = ftmp_template_seg
# if step>1, apply warp_forward_concat to the src image to be used
if i_step > 1:
# apply transformation from previous step, to use as new src for registration
sct_apply_transfo.main(args=[
'-i', src,
'-d', dest,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src = add_suffix(src, '_regStep' + str(i_step - 1))
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # also apply transformation to the seg
sct_apply_transfo.main(args=[
'-i', src_seg,
'-d', dest_seg,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src_seg = add_suffix(src_seg, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # im_seg case
warp_forward_out, warp_inverse_out = register([src, src_seg], [dest, dest_seg], paramreg, param, str(i_step))
else:
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.append(warp_inverse_out)
# Concatenate transformations: anat --> template
sct.printv('\nConcatenate transformations: anat --> template...', verbose)
warp_forward.insert(0, 'warp_curve2straightAffine.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_forward,
'-d', 'template.nii',
'-o', 'warp_anat2template.nii.gz'])
# Concatenate transformations: template --> anat
sct.printv('\nConcatenate transformations: template --> anat...', verbose)
warp_inverse.reverse()
if vertebral_alignment:
warp_inverse.append('warp_straight2curve.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
else:
warp_inverse.append('straight2templateAffine.txt')
warp_inverse.append('warp_straight2curve.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-winv', ['straight2templateAffine.txt'],
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
# register template->subject
elif ref == 'subject':
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Add one label because at least 3 orthogonal labels are required to estimate an affine transformation. This
# new label is added at the level of the upper most label (lowest value), at 1cm to the right.
for i_file in [ftmp_label, ftmp_template_label]:
im_label = Image(i_file)
coord_label = im_label.getCoordinatesAveragedByValue() # N.B. landmarks are sorted by value
# Create new label
from copy import deepcopy
new_label = deepcopy(coord_label[0])
# move it 5mm to the left (orientation is RAS)
nx, ny, nz, nt, px, py, pz, pt = im_label.dim
new_label.x = np.round(coord_label[0].x + 5.0 / px)
# assign value 99
new_label.value = 99
# Add to existing image
im_label.data[int(new_label.x), int(new_label.y), int(new_label.z)] = new_label.value
# Overwrite label file
# im_label.absolutepath = 'label_rpi_modif.nii.gz'
im_label.save()
# Bring template to subject space using landmark-based transformation
sct.printv('\nEstimate transformation for step #0...', verbose)
warp_forward = ['template2subjectAffine.txt']
warp_inverse = ['template2subjectAffine.txt']
try:
register_landmarks(ftmp_template_label, ftmp_label, paramreg.steps['0'].dof, fname_affine=warp_forward[0], verbose=verbose, path_qc="./")
except Exception:
sct.printv('ERROR: input labels do not seem to be at the right place. Please check the position of the labels. See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42', verbose=verbose, type='error')
# loop across registration steps
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_template
dest = ftmp_data
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_template_seg
dest = ftmp_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
# apply transformation from previous step, to use as new src for registration
sct_apply_transfo.main(args=[
'-i', src,
'-d', dest,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src = add_suffix(src, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: template --> subject...', verbose)
sct_concat_transfo.main(args=[
'-w', warp_forward,
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
sct.printv('\nConcatenate transformations: subject --> template...', verbose)
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-winv', ['template2subjectAffine.txt'],
'-d', 'template.nii',
'-o', 'warp_anat2template.nii.gz'])
# Apply warping fields to anat and template
sct.run(['sct_apply_transfo', '-i', 'template.nii', '-o', 'template2anat.nii.gz', '-d', 'data.nii', '-w', 'warp_template2anat.nii.gz', '-crop', '1'], verbose)
sct.run(['sct_apply_transfo', '-i', 'data.nii', '-o', 'anat2template.nii.gz', '-d', 'template.nii', '-w', 'warp_anat2template.nii.gz', '-crop', '1'], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
fname_template2anat = os.path.join(path_output, 'template2anat' + ext_data)
fname_anat2template = os.path.join(path_output, 'anat2template' + ext_data)
sct.generate_output_file(os.path.join(path_tmp, "warp_template2anat.nii.gz"), os.path.join(path_output, "warp_template2anat.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_anat2template.nii.gz"), os.path.join(path_output, "warp_anat2template.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "template2anat.nii.gz"), fname_template2anat, verbose)
sct.generate_output_file(os.path.join(path_tmp, "anat2template.nii.gz"), fname_anat2template, verbose)
if ref == 'template':
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose)
# Delete temporary files
if param.remove_temp_files:
sct.printv('\nDelete temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if param.path_qc is not None:
generate_qc(fname_data, fname_in2=fname_template2anat, fname_seg=fname_seg, args=args,
path_qc=os.path.abspath(param.path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_to_template')
sct.display_viewer_syntax([fname_data, fname_template2anat], verbose=verbose)
sct.display_viewer_syntax([fname_template, fname_anat2template], verbose=verbose)
def main(args=None):
# initializations
initz = ''
initcenter = ''
fname_initlabel = ''
file_labelz = 'labelz.nii.gz'
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_in = os.path.abspath(arguments["-i"])
fname_seg = os.path.abspath(arguments['-s'])
contrast = arguments['-c']
path_template = arguments['-t']
scale_dist = arguments['-scale-dist']
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = os.curdir
param.path_qc = arguments.get("-qc", None)
if '-discfile' in arguments:
fname_disc = os.path.abspath(arguments['-discfile'])
else:
fname_disc = None
if '-initz' in arguments:
initz = arguments['-initz']
if '-initcenter' in arguments:
initcenter = arguments['-initcenter']
# if user provided text file, parse and overwrite arguments
if '-initfile' in arguments:
file = open(arguments['-initfile'], 'r')
initfile = ' ' + file.read().replace('\n', '')
arg_initfile = initfile.split(' ')
for idx_arg, arg in enumerate(arg_initfile):
if arg == '-initz':
initz = [int(x) for x in arg_initfile[idx_arg + 1].split(',')]
if arg == '-initcenter':
initcenter = int(arg_initfile[idx_arg + 1])
if '-initlabel' in arguments:
# get absolute path of label
fname_initlabel = os.path.abspath(arguments['-initlabel'])
if '-param' in arguments:
param.update(arguments['-param'][0])
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
remove_temp_files = int(arguments['-r'])
denoise = int(arguments['-denoise'])
laplacian = int(arguments['-laplacian'])
path_tmp = sct.tmp_create(basename="label_vertebrae", verbose=verbose)
# Copying input data to tmp folder
sct.printv('\nCopying input data to tmp folder...', verbose)
Image(fname_in).save(os.path.join(path_tmp, "data.nii"))
Image(fname_seg).save(os.path.join(path_tmp, "segmentation.nii"))
# Go go temp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Straighten spinal cord
sct.printv('\nStraighten spinal cord...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
cache_sig = sct.cache_signature(
input_files=[fname_in, fname_seg],
)
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(os.path.join(curdir, "warp_curve2straight.nii.gz")) and os.path.isfile(os.path.join(curdir, "warp_straight2curve.nii.gz")) and os.path.isfile(os.path.join(curdir, "straight_ref.nii.gz")):
# if they exist, copy them into current folder
sct.printv('Reusing existing warping field which seems to be valid', verbose, 'warning')
sct.copy(os.path.join(curdir, "warp_curve2straight.nii.gz"), 'warp_curve2straight.nii.gz')
sct.copy(os.path.join(curdir, "warp_straight2curve.nii.gz"), 'warp_straight2curve.nii.gz')
sct.copy(os.path.join(curdir, "straight_ref.nii.gz"), 'straight_ref.nii.gz')
# apply straightening
s, o = sct.run(['sct_apply_transfo', '-i', 'data.nii', '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', 'data_straight.nii'])
else:
cmd = ['sct_straighten_spinalcord',
'-i', 'data.nii',
'-s', 'segmentation.nii',
'-r', str(remove_temp_files)]
if param.path_qc is not None and os.environ.get("SCT_RECURSIVE_QC", None) == "1":
cmd += ['-qc', param.path_qc]
s, o = sct.run(cmd)
sct.cache_save(cachefile, cache_sig)
# resample to 0.5mm isotropic to match template resolution
sct.printv('\nResample to 0.5mm isotropic...', verbose)
s, o = sct.run(['sct_resample', '-i', 'data_straight.nii', '-mm', '0.5x0.5x0.5', '-x', 'linear', '-o', 'data_straightr.nii'], verbose=verbose)
# Apply straightening to segmentation
# N.B. Output is RPI
sct.printv('\nApply straightening to segmentation...', verbose)
sct.run('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation.nii',
'data_straightr.nii',
'warp_curve2straight.nii.gz',
'segmentation_straight.nii',
'Linear'),
verbose=verbose,
is_sct_binary=True,
)
# Threshold segmentation at 0.5
sct.run(['sct_maths', '-i', 'segmentation_straight.nii', '-thr', '0.5', '-o', 'segmentation_straight.nii'], verbose)
# If disc label file is provided, label vertebrae using that file instead of automatically
if fname_disc:
# Apply straightening to disc-label
sct.printv('\nApply straightening to disc labels...', verbose)
sct.run('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
(fname_disc,
'data_straightr.nii',
'warp_curve2straight.nii.gz',
'labeldisc_straight.nii.gz',
'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
label_vert('segmentation_straight.nii', 'labeldisc_straight.nii.gz', verbose=1)
else:
# create label to identify disc
sct.printv('\nCreate label to identify disc...', verbose)
fname_labelz = os.path.join(path_tmp, file_labelz)
if initz or initcenter:
if initcenter:
# find z centered in FOV
nii = Image('segmentation.nii').change_orientation("RPI")
nx, ny, nz, nt, px, py, pz, pt = nii.dim # Get dimensions
z_center = int(np.round(nz / 2)) # get z_center
initz = [z_center, initcenter]
# create single label and output as labels.nii.gz
label = ProcessLabels('segmentation.nii', fname_output='tmp.labelz.nii.gz',
coordinates=['{},{}'.format(initz[0], initz[1])])
im_label = label.process('create-seg')
im_label.data = sct_maths.dilate(im_label.data, [3]) # TODO: create a dilation method specific to labels,
# which does not apply a convolution across all voxels (highly inneficient)
im_label.save(fname_labelz)
elif fname_initlabel:
import sct_label_utils
# subtract "1" to label value because due to legacy, in this code the disc C2-C3 has value "2", whereas in the
# recent version of SCT it is defined as "3". Therefore, when asking the user to define a label, we point to the
# new definition of labels (i.e., C2-C3 = 3).
sct_label_utils.main(['-i', fname_initlabel, '-add', '-1', '-o', fname_labelz])
else:
# automatically finds C2-C3 disc
im_data = Image('data.nii')
im_seg = Image('segmentation.nii')
im_label_c2c3 = detect_c2c3(im_data, im_seg, contrast)
ind_label = np.where(im_label_c2c3.data)
if not np.size(ind_label) == 0:
# subtract "1" to label value because due to legacy, in this code the disc C2-C3 has value "2", whereas in the
# recent version of SCT it is defined as "3".
im_label_c2c3.data[ind_label] = 2
else:
sct.printv('Automatic C2-C3 detection failed. Please provide manual label with sct_label_utils', 1, 'error')
im_label_c2c3.save(fname_labelz)
# dilate label so it is not lost when applying warping
sct_maths.main(['-i', fname_labelz, '-dilate', '3', '-o', fname_labelz])
# Apply straightening to z-label
sct.printv('\nAnd apply straightening to label...', verbose)
sct.run('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
(file_labelz,
'data_straightr.nii',
'warp_curve2straight.nii.gz',
'labelz_straight.nii.gz',
'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
# get z value and disk value to initialize labeling
sct.printv('\nGet z and disc values from straight label...', verbose)
init_disc = get_z_and_disc_values_from_label('labelz_straight.nii.gz')
sct.printv('.. ' + str(init_disc), verbose)
# denoise data
if denoise:
sct.printv('\nDenoise data...', verbose)
sct.run(['sct_maths', '-i', 'data_straightr.nii', '-denoise', 'h=0.05', '-o', 'data_straightr.nii'], verbose)
# apply laplacian filtering
if laplacian:
sct.printv('\nApply Laplacian filter...', verbose)
sct.run(['sct_maths', '-i', 'data_straightr.nii', '-laplacian', '1', '-o', 'data_straightr.nii'], verbose)
# detect vertebral levels on straight spinal cord
vertebral_detection('data_straightr.nii', 'segmentation_straight.nii', contrast, param, init_disc=init_disc,
verbose=verbose, path_template=path_template, path_output=path_output, scale_dist=scale_dist)
# un-straighten labeled spinal cord
sct.printv('\nUn-straighten labeling...', verbose)
sct.run('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation_straight_labeled.nii',
'segmentation.nii',
'warp_straight2curve.nii.gz',
'segmentation_labeled.nii',
'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
# Clean labeled segmentation
sct.printv('\nClean labeled segmentation (correct interpolation errors)...', verbose)
clean_labeled_segmentation('segmentation_labeled.nii', 'segmentation.nii', 'segmentation_labeled.nii')
# label discs
sct.printv('\nLabel discs...', verbose)
label_discs('segmentation_labeled.nii', verbose=verbose)
# come back
os.chdir(curdir)
# Generate output files
path_seg, file_seg, ext_seg = sct.extract_fname(fname_seg)
fname_seg_labeled = os.path.join(path_output, file_seg + '_labeled' + ext_seg)
sct.printv('\nGenerate output files...', verbose)
sct.generate_output_file(os.path.join(path_tmp, "segmentation_labeled.nii"), fname_seg_labeled)
sct.generate_output_file(os.path.join(path_tmp, "segmentation_labeled_disc.nii"), os.path.join(path_output, file_seg + '_labeled_discs' + ext_seg))
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose)
# Remove temporary files
if remove_temp_files == 1:
sct.printv('\nRemove temporary files...', verbose)
sct.rmtree(path_tmp)
# Generate QC report
if param.path_qc is not None:
path_qc = os.path.abspath(param.path_qc)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
labeled_seg_file = os.path.join(path_output, file_seg + '_labeled' + ext_seg)
generate_qc(fname_in, fname_seg=labeled_seg_file, args=args, path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_label_vertebrae')
sct.display_viewer_syntax([fname_in, fname_seg_labeled], colormaps=['', 'subcortical'], opacities=['1', '0.5'])
def main(args=None):
parser = get_parser()
if args:
arguments = parser.parse_args(args)
else:
arguments = parser.parse_args(args=None if sys.argv[1:] else ['--help'])
verbosity = arguments.v
init_sct(log_level=verbosity, update=True) # Update log level
input_filename = arguments.i
output_fname = arguments.o
img = Image(input_filename)
dtype = None
if arguments.add is not None:
value = arguments.add
out = sct_labels.add(img, value)
elif arguments.create is not None:
labels = arguments.create
out = sct_labels.create_labels_empty(img, labels)
elif arguments.create_add is not None:
labels = arguments.create_add
out = sct_labels.create_labels(img, labels)
elif arguments.create_seg is not None:
labels = arguments.create_seg
out = sct_labels.create_labels_along_segmentation(img, labels)
elif arguments.cubic_to_point:
out = sct_labels.cubic_to_point(img)
elif arguments.display:
display_voxel(img, verbosity)
return
elif arguments.increment:
out = sct_labels.increment_z_inverse(img)
elif arguments.disc is not None:
ref = Image(arguments.disc)
out = sct_labels.labelize_from_discs(img, ref)
elif arguments.vert_body is not None:
levels = arguments.vert_body
if len(levels) == 1 and levels[0] == 0:
levels = None # all levels
out = sct_labels.label_vertebrae(img, levels)
elif arguments.vert_continuous:
out = sct_labels.continuous_vertebral_levels(img)
dtype = 'float32'
elif arguments.MSE is not None:
ref = Image(arguments.MSE)
mse = sct_labels.compute_mean_squared_error(img, ref)
printv(f"Computed MSE: {mse}")
return
elif arguments.remove_reference is not None:
ref = Image(arguments.remove_reference)
out = sct_labels.remove_missing_labels(img, ref)
elif arguments.remove_sym is not None:
# first pass use img as source
ref = Image(arguments.remove_reference)
out = sct_labels.remove_missing_labels(img, ref)
# second pass use previous pass result as reference
ref_out = sct_labels.remove_missing_labels(ref, out)
ref_out.save(path=ref.absolutepath)
elif arguments.remove is not None:
labels = arguments.remove
out = sct_labels.remove_labels_from_image(img, labels)
elif arguments.keep is not None:
labels = arguments.keep
out = sct_labels.remove_other_labels_from_image(img, labels)
elif arguments.create_viewer is not None:
msg = "" if arguments.msg is None else f"{arguments.msg}\n"
if arguments.ilabel is not None:
input_labels_img = Image(arguments.ilabel)
out = launch_manual_label_gui(img, input_labels_img, parse_num_list(arguments.create_viewer), msg)
else:
out = launch_sagittal_viewer(img, parse_num_list(arguments.create_viewer), msg)
printv("Generating output files...")
out.save(path=output_fname, dtype=dtype)
display_viewer_syntax([input_filename, output_fname])
if arguments.qc is not None:
generate_qc(fname_in1=input_filename, fname_seg=output_fname, args=args,
path_qc=os.path.abspath(arguments.qc), dataset=arguments.qc_dataset,
subject=arguments.qc_subject, process='sct_label_utils')
def main(args=None):
if args is None:
args = sys.argv[1:]
# initialize parameters
param = Param()
# Initialization
fname_output = ''
path_out = ''
fname_src_seg = ''
fname_dest_seg = ''
fname_src_label = ''
fname_dest_label = ''
generate_warpinv = 1
start_time = time.time()
# get default registration parameters
# step1 = Paramreg(step='1', type='im', algo='syn', metric='MI', iter='5', shrink='1', smooth='0', gradStep='0.5')
step0 = Paramreg(step='0', type='im', algo='syn', metric='MI', iter='0', shrink='1', smooth='0', gradStep='0.5',
slicewise='0', dof='Tx_Ty_Tz_Rx_Ry_Rz') # only used to put src into dest space
step1 = Paramreg(step='1', type='im')
paramreg = ParamregMultiStep([step0, step1])
parser = get_parser(paramreg=paramreg)
arguments = parser.parse(args)
# get arguments
fname_src = arguments['-i']
fname_dest = arguments['-d']
if '-iseg' in arguments:
fname_src_seg = arguments['-iseg']
if '-dseg' in arguments:
fname_dest_seg = arguments['-dseg']
if '-ilabel' in arguments:
fname_src_label = arguments['-ilabel']
if '-dlabel' in arguments:
fname_dest_label = arguments['-dlabel']
if '-o' in arguments:
fname_output = arguments['-o']
if '-ofolder' in arguments:
path_out = arguments['-ofolder']
if '-owarp' in arguments:
fname_output_warp = arguments['-owarp']
else:
fname_output_warp = ''
if '-initwarp' in arguments:
fname_initwarp = os.path.abspath(arguments['-initwarp'])
else:
fname_initwarp = ''
if '-initwarpinv' in arguments:
fname_initwarpinv = os.path.abspath(arguments['-initwarpinv'])
else:
fname_initwarpinv = ''
if '-m' in arguments:
fname_mask = arguments['-m']
else:
fname_mask = ''
padding = arguments['-z']
if "-param" in arguments:
paramreg_user = arguments['-param']
# update registration parameters
for paramStep in paramreg_user:
paramreg.addStep(paramStep)
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
identity = int(arguments['-identity'])
interp = arguments['-x']
remove_temp_files = int(arguments['-r'])
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
# sct.printv(arguments)
sct.printv('\nInput parameters:')
sct.printv(' Source .............. ' + fname_src)
sct.printv(' Destination ......... ' + fname_dest)
sct.printv(' Init transfo ........ ' + fname_initwarp)
sct.printv(' Mask ................ ' + fname_mask)
sct.printv(' Output name ......... ' + fname_output)
# sct.printv(' Algorithm ........... '+paramreg.algo)
# sct.printv(' Number of iterations '+paramreg.iter)
# sct.printv(' Metric .............. '+paramreg.metric)
sct.printv(' Remove temp files ... ' + str(remove_temp_files))
sct.printv(' Verbose ............. ' + str(verbose))
# update param
param.verbose = verbose
param.padding = padding
param.fname_mask = fname_mask
param.remove_temp_files = remove_temp_files
# Get if input is 3D
sct.printv('\nCheck if input data are 3D...', verbose)
sct.check_if_3d(fname_src)
sct.check_if_3d(fname_dest)
# Check if user selected type=seg, but did not input segmentation data
if 'paramreg_user' in locals():
if True in ['type=seg' in paramreg_user[i] for i in range(len(paramreg_user))]:
if fname_src_seg == '' or fname_dest_seg == '':
sct.printv('\nERROR: if you select type=seg you must specify -iseg and -dseg flags.\n', 1, 'error')
# Extract path, file and extension
path_src, file_src, ext_src = sct.extract_fname(fname_src)
path_dest, file_dest, ext_dest = sct.extract_fname(fname_dest)
# check if source and destination images have the same name (related to issue #373)
# If so, change names to avoid conflict of result files and warns the user
suffix_src, suffix_dest = '_reg', '_reg'
if file_src == file_dest:
suffix_src, suffix_dest = '_src_reg', '_dest_reg'
# define output folder and file name
if fname_output == '':
path_out = '' if not path_out else path_out # output in user's current directory
file_out = file_src + suffix_src
file_out_inv = file_dest + suffix_dest
ext_out = ext_src
else:
path, file_out, ext_out = sct.extract_fname(fname_output)
path_out = path if not path_out else path_out
file_out_inv = file_out + '_inv'
# create temporary folder
path_tmp = sct.tmp_create()
sct.printv('\nCopying input data to tmp folder and convert to nii...', verbose)
Image(fname_src).save(os.path.join(path_tmp, "src.nii"))
Image(fname_dest).save(os.path.join(path_tmp, "dest.nii"))
if fname_src_seg:
Image(fname_src_seg).save(os.path.join(path_tmp, "src_seg.nii"))
if fname_dest_seg:
Image(fname_dest_seg).save(os.path.join(path_tmp, "dest_seg.nii"))
if fname_src_label:
Image(fname_src_label).save(os.path.join(path_tmp, "src_label.nii"))
Image(fname_dest_label).save(os.path.join(path_tmp, "dest_label.nii"))
if fname_mask != '':
Image(fname_mask).save(os.path.join(path_tmp, "mask.nii.gz"))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# reorient destination to RPI
Image('dest.nii').change_orientation("RPI").save('dest_RPI.nii')
if fname_dest_seg:
Image('dest_seg.nii').change_orientation("RPI").save('dest_seg_RPI.nii')
if fname_dest_label:
Image('dest_label.nii').change_orientation("RPI").save('dest_label_RPI.nii')
if identity:
# overwrite paramreg and only do one identity transformation
step0 = Paramreg(step='0', type='im', algo='syn', metric='MI', iter='0', shrink='1', smooth='0', gradStep='0.5')
paramreg = ParamregMultiStep([step0])
# Put source into destination space using header (no estimation -- purely based on header)
# TODO: Check if necessary to do that
# TODO: use that as step=0
# sct.printv('\nPut source into destination space using header...', verbose)
# sct.run('isct_antsRegistration -d 3 -t Translation[0] -m MI[dest_pad.nii,src.nii,1,16] -c 0 -f 1 -s 0 -o
# [regAffine,src_regAffine.nii] -n BSpline[3]', verbose)
# if segmentation, also do it for seg
# initialize list of warping fields
warp_forward = []
warp_inverse = []
# initial warping is specified, update list of warping fields and skip step=0
if fname_initwarp:
sct.printv('\nSkip step=0 and replace with initial transformations: ', param.verbose)
sct.printv(' ' + fname_initwarp, param.verbose)
# sct.copy(fname_initwarp, 'warp_forward_0.nii.gz')
warp_forward = [fname_initwarp]
start_step = 1
if fname_initwarpinv:
warp_inverse = [fname_initwarpinv]
else:
sct.printv('\nWARNING: No initial inverse warping field was specified, therefore the inverse warping field '
'will NOT be generated.', param.verbose, 'warning')
generate_warpinv = 0
else:
start_step = 0
# loop across registration steps
for i_step in range(start_step, len(paramreg.steps)):
sct.printv('\n--\nESTIMATE TRANSFORMATION FOR STEP #' + str(i_step), param.verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = 'src.nii'
dest = 'dest_RPI.nii'
interp_step = 'spline'
elif paramreg.steps[str(i_step)].type == 'seg':
src = 'src_seg.nii'
dest = 'dest_seg_RPI.nii'
interp_step = 'nn'
elif paramreg.steps[str(i_step)].type == 'label':
src = 'src_label.nii'
dest = 'dest_label_RPI.nii'
interp_step = 'nn'
else:
# src = dest = interp_step = None
sct.printv('ERROR: Wrong image type.', 1, 'error')
# if step>0, apply warp_forward_concat to the src image to be used
if i_step > 0:
sct.printv('\nApply transformation from previous step', param.verbose)
sct.run(['sct_apply_transfo', '-i', src, '-d', dest, '-w', ','.join(warp_forward), '-o',
sct.add_suffix(src, '_reg'), '-x', interp_step], verbose)
src = sct.add_suffix(src, '_reg')
# register src --> dest
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations
sct.printv('\nConcatenate transformations...', verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_forward), '-d', 'dest.nii', '-o', 'warp_src2dest.nii.gz'],
verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse), '-d', 'src.nii', '-o', 'warp_dest2src.nii.gz'],
verbose)
# Apply warping field to src data
sct.printv('\nApply transfo source --> dest...', verbose)
sct.run(['sct_apply_transfo', '-i', 'src.nii', '-o', 'src_reg.nii', '-d', 'dest.nii', '-w', 'warp_src2dest.nii.gz',
'-x', interp], verbose)
sct.printv('\nApply transfo dest --> source...', verbose)
sct.run(['sct_apply_transfo', '-i', 'dest.nii', '-o', 'dest_reg.nii', '-d', 'src.nii', '-w', 'warp_dest2src.nii.gz',
'-x', interp], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
# generate: src_reg
fname_src2dest = sct.generate_output_file(os.path.join(path_tmp, "src_reg.nii"),
os.path.join(path_out, file_out + ext_out), verbose)
# generate: forward warping field
if fname_output_warp == '':
fname_output_warp = os.path.join(path_out, 'warp_' + file_src + '2' + file_dest + '.nii.gz')
sct.generate_output_file(os.path.join(path_tmp, "warp_src2dest.nii.gz"), fname_output_warp, verbose)
if generate_warpinv:
# generate: dest_reg
fname_dest2src = sct.generate_output_file(os.path.join(path_tmp, "dest_reg.nii"),
os.path.join(path_out, file_out_inv + ext_dest), verbose)
# generate: inverse warping field
sct.generate_output_file(os.path.join(path_tmp, "warp_dest2src.nii.gz"),
os.path.join(path_out, 'warp_' + file_dest + '2' + file_src + '.nii.gz'), verbose)
# Delete temporary files
if remove_temp_files:
sct.printv('\nRemove temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
if path_qc is not None:
if fname_dest_seg:
generate_qc(fname_src2dest, fname_in2=fname_dest, fname_seg=fname_dest_seg, args=args,
path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_multimodal')
else:
sct.printv('WARNING: Cannot generate QC because it requires destination segmentation.', 1, 'warning')
if generate_warpinv:
sct.display_viewer_syntax([fname_src, fname_dest2src], verbose=verbose)
sct.display_viewer_syntax([fname_dest, fname_src2dest], verbose=verbose)
def main(args):
parser = get_parser()
arguments = parser.parse(args)
# Initialization
slices = ''
group_funcs = (('MEAN', func_wa), ('STD', func_std)) # functions to perform when aggregating metrics along S-I
fname_segmentation = sct.get_absolute_path(arguments['-i'])
fname_vert_levels = ''
if '-o' in arguments:
file_out = os.path.abspath(arguments['-o'])
else:
file_out = ''
if '-append' in arguments:
append = int(arguments['-append'])
else:
append = 0
if '-vert' in arguments:
vert_levels = arguments['-vert']
else:
vert_levels = ''
if '-r' in arguments:
remove_temp_files = arguments['-r']
if '-vertfile' in arguments:
fname_vert_levels = arguments['-vertfile']
if '-perlevel' in arguments:
perlevel = arguments['-perlevel']
else:
perlevel = None
if '-z' in arguments:
slices = arguments['-z']
if '-perslice' in arguments:
perslice = arguments['-perslice']
else:
perslice = None
if '-angle-corr' in arguments:
if arguments['-angle-corr'] == '1':
angle_correction = True
elif arguments['-angle-corr'] == '0':
angle_correction = False
param_centerline = ParamCenterline(
algo_fitting=arguments['-centerline-algo'],
smooth=arguments['-centerline-smooth'],
minmax=True)
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
# update fields
metrics_agg = {}
if not file_out:
file_out = 'csa.csv'
metrics, fit_results = process_seg.compute_shape(fname_segmentation,
angle_correction=angle_correction,
param_centerline=param_centerline,
verbose=verbose)
for key in metrics:
metrics_agg[key] = aggregate_per_slice_or_level(metrics[key], slices=parse_num_list(slices),
levels=parse_num_list(vert_levels), perslice=perslice,
perlevel=perlevel, vert_level=fname_vert_levels,
group_funcs=group_funcs)
metrics_agg_merged = _merge_dict(metrics_agg)
save_as_csv(metrics_agg_merged, file_out, fname_in=fname_segmentation, append=append)
# QC report (only show CSA for clarity)
if path_qc is not None:
generate_qc(fname_segmentation, args=args, path_qc=os.path.abspath(path_qc), dataset=qc_dataset,
subject=qc_subject, path_img=_make_figure(metrics_agg_merged, fit_results),
process='sct_process_segmentation')
sct.display_open(file_out)
def main():
"""Main function."""
parser = get_parser()
args = parser.parse_args(args=None if sys.argv[1:] else ['--help'])
fname_image = os.path.abspath(args.i)
contrast_type = args.c
ctr_algo = args.centerline
if args.brain is None:
if contrast_type in ['t2s', 'dwi']:
brain_bool = False
if contrast_type in ['t1', 't2']:
brain_bool = True
else:
brain_bool = bool(args.brain)
if bool(args.brain) and ctr_algo == 'svm':
sct.printv(
'Please only use the flag "-brain 1" with "-centerline cnn".', 1,
'warning')
sys.exit(1)
kernel_size = args.kernel
if kernel_size == '3d' and contrast_type == 'dwi':
kernel_size = '2d'
sct.printv(
'3D kernel model for dwi contrast is not available. 2D kernel model is used instead.',
type="warning")
if ctr_algo == 'file' and args.file_centerline is None:
sct.printv(
'Please use the flag -file_centerline to indicate the centerline filename.',
1, 'warning')
sys.exit(1)
if args.file_centerline is not None:
manual_centerline_fname = args.file_centerline
ctr_algo = 'file'
else:
manual_centerline_fname = None
threshold = args.thr
if threshold is not None:
if threshold > 1.0 or (threshold < 0.0 and threshold != -1.0):
raise SyntaxError(
"Threshold should be between 0 and 1, or equal to -1 (no threshold)"
)
remove_temp_files = args.r
verbose = args.v
init_sct(log_level=verbose, update=True) # Update log level
path_qc = args.qc
qc_dataset = args.qc_dataset
qc_subject = args.qc_subject
output_folder = args.ofolder
# check if input image is 2D or 3D
sct.check_dim(fname_image, dim_lst=[2, 3])
# Segment image
from spinalcordtoolbox.image import Image
from spinalcordtoolbox.deepseg_sc.core import deep_segmentation_spinalcord
from spinalcordtoolbox.reports.qc import generate_qc
im_image = Image(fname_image)
# note: below we pass im_image.copy() otherwise the field absolutepath becomes None after execution of this function
im_seg, im_image_RPI_upsamp, im_seg_RPI_upsamp = \
deep_segmentation_spinalcord(im_image.copy(), contrast_type, ctr_algo=ctr_algo,
ctr_file=manual_centerline_fname, brain_bool=brain_bool, kernel_size=kernel_size,
threshold_seg=threshold, remove_temp_files=remove_temp_files, verbose=verbose)
# Save segmentation
fname_seg = os.path.abspath(
os.path.join(
output_folder,
sct.extract_fname(fname_image)[1] + '_seg' +
sct.extract_fname(fname_image)[2]))
im_seg.save(fname_seg)
# Generate QC report
if path_qc is not None:
generate_qc(fname_image,
fname_seg=fname_seg,
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_deepseg_sc')
sct.display_viewer_syntax([fname_image, fname_seg],
colormaps=['gray', 'red'],
opacities=['', '0.7'])
def main():
"""Main function."""
sct.init_sct()
parser = get_parser()
args = sys.argv[1:]
arguments = parser.parse(args)
fname_image = os.path.abspath(arguments['-i'])
contrast_type = arguments['-c']
ctr_algo = arguments["-centerline"]
if "-brain" not in args:
if contrast_type in ['t2s', 'dwi']:
brain_bool = False
if contrast_type in ['t1', 't2']:
brain_bool = True
else:
brain_bool = bool(int(arguments["-brain"]))
kernel_size = arguments["-kernel"]
if kernel_size == '3d' and contrast_type == 'dwi':
kernel_size = '2d'
sct.printv('3D kernel model for dwi contrast is not available. 2D kernel model is used instead.', type="warning")
if '-ofolder' not in args:
output_folder = os.getcwd()
else:
output_folder = arguments["-ofolder"]
if ctr_algo == 'file' and "-file_centerline" not in args:
sct.log.warning('Please use the flag -file_centerline to indicate the centerline filename.')
sys.exit(1)
if "-file_centerline" in args:
manual_centerline_fname = arguments["-file_centerline"]
ctr_algo = 'file'
else:
manual_centerline_fname = None
remove_temp_files = int(arguments['-r'])
verbose = int(arguments['-v'])
path_qc = arguments.get("-qc", None)
algo_config_stg = '\nMethod:'
algo_config_stg += '\n\tCenterline algorithm: ' + str(ctr_algo)
algo_config_stg += '\n\tAssumes brain section included in the image: ' + str(brain_bool)
algo_config_stg += '\n\tDimension of the segmentation kernel convolutions: ' + kernel_size + '\n'
sct.printv(algo_config_stg)
im_image = Image(fname_image)
# note: below we pass im_image.copy() otherwise the field absolutepath becomes None after execution of this function
im_seg, im_image_RPI_upsamp, im_seg_RPI_upsamp, im_labels_viewer, im_ctr = deep_segmentation_spinalcord(
im_image.copy(), contrast_type, ctr_algo=ctr_algo, ctr_file=manual_centerline_fname,
brain_bool=brain_bool, kernel_size=kernel_size, remove_temp_files=remove_temp_files, verbose=verbose)
# Save segmentation
fname_seg = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_seg' +
sct.extract_fname(fname_image)[2]))
im_seg.save(fname_seg)
if ctr_algo == 'viewer':
# Save labels
fname_labels = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_labels-centerline' +
sct.extract_fname(fname_image)[2]))
im_labels_viewer.save(fname_labels)
if verbose == 2:
# Save ctr
fname_ctr = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_centerline' +
sct.extract_fname(fname_image)[2]))
im_ctr.save(fname_ctr)
if path_qc is not None:
generate_qc(fname_image, fname_seg=fname_seg, args=args, path_qc=os.path.abspath(path_qc),
process='sct_deepseg_sc')
sct.display_viewer_syntax([fname_image, fname_seg], colormaps=['gray', 'red'], opacities=['', '0.7'])
def main():
"""Main function."""
parser = get_parser()
args = parser.parse_args(args=None if sys.argv[1:] else ['--help'])
fname_image = os.path.abspath(args.i)
contrast_type = args.c
ctr_algo = args.centerline
if args.brain is None:
if contrast_type in ['t2s', 'dwi']:
brain_bool = False
if contrast_type in ['t1', 't2']:
brain_bool = True
else:
brain_bool = bool(args.brain)
kernel_size = args.kernel
if kernel_size == '3d' and contrast_type == 'dwi':
kernel_size = '2d'
sct.printv('3D kernel model for dwi contrast is not available. 2D kernel model is used instead.',
type="warning")
if ctr_algo == 'file' and args.file_centerline is None:
sct.printv('Please use the flag -file_centerline to indicate the centerline filename.', 1, 'warning')
sys.exit(1)
if args.file_centerline is not None:
manual_centerline_fname = args.file_centerline
ctr_algo = 'file'
else:
manual_centerline_fname = None
remove_temp_files = args.r
verbose = args.v
sct.init_sct(log_level=verbose, update=True) # Update log level
path_qc = args.qc
qc_dataset = args.qc_dataset
qc_subject = args.qc_subject
output_folder = args.ofolder
algo_config_stg = '\nMethod:'
algo_config_stg += '\n\tCenterline algorithm: ' + str(ctr_algo)
algo_config_stg += '\n\tAssumes brain section included in the image: ' + str(brain_bool)
algo_config_stg += '\n\tDimension of the segmentation kernel convolutions: ' + kernel_size + '\n'
sct.printv(algo_config_stg)
# Segment image
from spinalcordtoolbox.image import Image
from spinalcordtoolbox.deepseg_sc.core import deep_segmentation_spinalcord
from spinalcordtoolbox.reports.qc import generate_qc
im_image = Image(fname_image)
# note: below we pass im_image.copy() otherwise the field absolutepath becomes None after execution of this function
im_seg, im_image_RPI_upsamp, im_seg_RPI_upsamp, im_labels_viewer, im_ctr = \
deep_segmentation_spinalcord(im_image.copy(), contrast_type, ctr_algo=ctr_algo,
ctr_file=manual_centerline_fname, brain_bool=brain_bool, kernel_size=kernel_size,
remove_temp_files=remove_temp_files, verbose=verbose)
# Save segmentation
fname_seg = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_seg' +
sct.extract_fname(fname_image)[2]))
# copy q/sform from input image to output segmentation
im_seg.copy_qform_from_ref(im_image)
im_seg.save(fname_seg)
if ctr_algo == 'viewer':
# Save labels
fname_labels = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_labels-centerline' +
sct.extract_fname(fname_image)[2]))
im_labels_viewer.save(fname_labels)
if verbose == 2:
# Save ctr
fname_ctr = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_centerline' +
sct.extract_fname(fname_image)[2]))
im_ctr.save(fname_ctr)
if path_qc is not None:
generate_qc(fname_image, fname_seg=fname_seg, args=sys.argv[1:], path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_deepseg_sc')
sct.display_viewer_syntax([fname_image, fname_seg], colormaps=['gray', 'red'], opacities=['', '0.7'])
def main():
"""Main function."""
sct.init_sct()
parser = get_parser()
args = sys.argv[1:]
arguments = parser.parse(args)
fname_image = os.path.abspath(arguments['-i'])
contrast_type = arguments['-c']
ctr_algo = arguments["-centerline"]
if "-brain" not in args:
if contrast_type in ['t2s', 'dwi']:
brain_bool = False
if contrast_type in ['t1', 't2']:
brain_bool = True
else:
brain_bool = bool(int(arguments["-brain"]))
kernel_size = arguments["-kernel"]
if kernel_size == '3d' and contrast_type == 'dwi':
kernel_size = '2d'
sct.printv('3D kernel model for dwi contrast is not available. 2D kernel model is used instead.', type="warning")
if '-ofolder' not in args:
output_folder = os.getcwd()
else:
output_folder = arguments["-ofolder"]
if ctr_algo == 'file' and "-file_centerline" not in args:
logger.warning('Please use the flag -file_centerline to indicate the centerline filename.')
sys.exit(1)
if "-file_centerline" in args:
manual_centerline_fname = arguments["-file_centerline"]
ctr_algo = 'file'
else:
manual_centerline_fname = None
remove_temp_files = int(arguments['-r'])
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
algo_config_stg = '\nMethod:'
algo_config_stg += '\n\tCenterline algorithm: ' + str(ctr_algo)
algo_config_stg += '\n\tAssumes brain section included in the image: ' + str(brain_bool)
algo_config_stg += '\n\tDimension of the segmentation kernel convolutions: ' + kernel_size + '\n'
sct.printv(algo_config_stg)
im_image = Image(fname_image)
# note: below we pass im_image.copy() otherwise the field absolutepath becomes None after execution of this function
im_seg, im_image_RPI_upsamp, im_seg_RPI_upsamp, im_labels_viewer, im_ctr = deep_segmentation_spinalcord(
im_image.copy(), contrast_type, ctr_algo=ctr_algo, ctr_file=manual_centerline_fname,
brain_bool=brain_bool, kernel_size=kernel_size, remove_temp_files=remove_temp_files, verbose=verbose)
# Save segmentation
fname_seg = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_seg' +
sct.extract_fname(fname_image)[2]))
im_seg.save(fname_seg)
if ctr_algo == 'viewer':
# Save labels
fname_labels = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_labels-centerline' +
sct.extract_fname(fname_image)[2]))
im_labels_viewer.save(fname_labels)
if verbose == 2:
# Save ctr
fname_ctr = os.path.abspath(os.path.join(output_folder, sct.extract_fname(fname_image)[1] + '_centerline' +
sct.extract_fname(fname_image)[2]))
im_ctr.save(fname_ctr)
if path_qc is not None:
generate_qc(fname_image, fname_seg=fname_seg, args=args, path_qc=os.path.abspath(path_qc),
dataset=qc_dataset, subject=qc_subject, process='sct_deepseg_sc')
sct.display_viewer_syntax([fname_image, fname_seg], colormaps=['gray', 'red'], opacities=['', '0.7'])
def main(args=None):
if args is None:
args = sys.argv[1:]
# initialize parameters
param = Param()
# Initialization
fname_output = ''
path_out = ''
fname_src_seg = ''
fname_dest_seg = ''
fname_src_label = ''
fname_dest_label = ''
generate_warpinv = 1
start_time = time.time()
# get path of the toolbox
path_sct = os.environ.get("SCT_DIR",
os.path.dirname(os.path.dirname(__file__)))
# get default registration parameters
# step1 = Paramreg(step='1', type='im', algo='syn', metric='MI', iter='5', shrink='1', smooth='0', gradStep='0.5')
step0 = Paramreg(
step='0',
type='im',
algo='syn',
metric='MI',
iter='0',
shrink='1',
smooth='0',
gradStep='0.5',
slicewise='0',
dof='Tx_Ty_Tz_Rx_Ry_Rz') # only used to put src into dest space
step1 = Paramreg(step='1', type='im')
paramreg = ParamregMultiStep([step0, step1])
parser = get_parser(paramreg=paramreg)
arguments = parser.parse(args)
# get arguments
fname_src = arguments['-i']
fname_dest = arguments['-d']
if '-iseg' in arguments:
fname_src_seg = arguments['-iseg']
if '-dseg' in arguments:
fname_dest_seg = arguments['-dseg']
if '-ilabel' in arguments:
fname_src_label = arguments['-ilabel']
if '-dlabel' in arguments:
fname_dest_label = arguments['-dlabel']
if '-o' in arguments:
fname_output = arguments['-o']
if '-ofolder' in arguments:
path_out = arguments['-ofolder']
if '-owarp' in arguments:
fname_output_warp = arguments['-owarp']
else:
fname_output_warp = ''
if '-initwarp' in arguments:
fname_initwarp = os.path.abspath(arguments['-initwarp'])
else:
fname_initwarp = ''
if '-initwarpinv' in arguments:
fname_initwarpinv = os.path.abspath(arguments['-initwarpinv'])
else:
fname_initwarpinv = ''
if '-m' in arguments:
fname_mask = arguments['-m']
else:
fname_mask = ''
padding = arguments['-z']
if "-param" in arguments:
paramreg_user = arguments['-param']
# update registration parameters
for paramStep in paramreg_user:
paramreg.addStep(paramStep)
path_qc = arguments.get("-qc", None)
identity = int(arguments['-identity'])
interp = arguments['-x']
remove_temp_files = int(arguments['-r'])
verbose = int(arguments['-v'])
# sct.printv(arguments)
sct.printv('\nInput parameters:')
sct.printv(' Source .............. ' + fname_src)
sct.printv(' Destination ......... ' + fname_dest)
sct.printv(' Init transfo ........ ' + fname_initwarp)
sct.printv(' Mask ................ ' + fname_mask)
sct.printv(' Output name ......... ' + fname_output)
# sct.printv(' Algorithm ........... '+paramreg.algo)
# sct.printv(' Number of iterations '+paramreg.iter)
# sct.printv(' Metric .............. '+paramreg.metric)
sct.printv(' Remove temp files ... ' + str(remove_temp_files))
sct.printv(' Verbose ............. ' + str(verbose))
# update param
param.verbose = verbose
param.padding = padding
param.fname_mask = fname_mask
param.remove_temp_files = remove_temp_files
# Get if input is 3D
sct.printv('\nCheck if input data are 3D...', verbose)
sct.check_if_3d(fname_src)
sct.check_if_3d(fname_dest)
# Check if user selected type=seg, but did not input segmentation data
if 'paramreg_user' in locals():
if True in [
'type=seg' in paramreg_user[i]
for i in range(len(paramreg_user))
]:
if fname_src_seg == '' or fname_dest_seg == '':
sct.printv(
'\nERROR: if you select type=seg you must specify -iseg and -dseg flags.\n',
1, 'error')
# Extract path, file and extension
path_src, file_src, ext_src = sct.extract_fname(fname_src)
path_dest, file_dest, ext_dest = sct.extract_fname(fname_dest)
# check if source and destination images have the same name (related to issue #373)
# If so, change names to avoid conflict of result files and warns the user
suffix_src, suffix_dest = '_reg', '_reg'
if file_src == file_dest:
suffix_src, suffix_dest = '_src_reg', '_dest_reg'
# define output folder and file name
if fname_output == '':
path_out = '' if not path_out else path_out # output in user's current directory
file_out = file_src + suffix_src
file_out_inv = file_dest + suffix_dest
ext_out = ext_src
else:
path, file_out, ext_out = sct.extract_fname(fname_output)
path_out = path if not path_out else path_out
file_out_inv = file_out + '_inv'
# create temporary folder
path_tmp = sct.tmp_create()
sct.printv('\nCopying input data to tmp folder and convert to nii...',
verbose)
Image(fname_src).save(os.path.join(path_tmp, "src.nii"))
Image(fname_dest).save(os.path.join(path_tmp, "dest.nii"))
if fname_src_seg:
Image(fname_src_seg).save(os.path.join(path_tmp, "src_seg.nii"))
if fname_dest_seg:
Image(fname_dest_seg).save(os.path.join(path_tmp, "dest_seg.nii"))
if fname_src_label:
Image(fname_src_label).save(os.path.join(path_tmp, "src_label.nii"))
Image(fname_dest_label).save(os.path.join(path_tmp, "dest_label.nii"))
if fname_mask != '':
Image(fname_mask).save(os.path.join(path_tmp, "mask.nii.gz"))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# reorient destination to RPI
Image('dest.nii').change_orientation("RPI").save('dest_RPI.nii')
if fname_dest_seg:
Image('dest_seg.nii').change_orientation("RPI").save(
'dest_seg_RPI.nii')
if fname_dest_label:
Image('dest_label.nii').change_orientation("RPI").save(
'dest_label_RPI.nii')
if identity:
# overwrite paramreg and only do one identity transformation
step0 = Paramreg(step='0',
type='im',
algo='syn',
metric='MI',
iter='0',
shrink='1',
smooth='0',
gradStep='0.5')
paramreg = ParamregMultiStep([step0])
# Put source into destination space using header (no estimation -- purely based on header)
# TODO: Check if necessary to do that
# TODO: use that as step=0
# sct.printv('\nPut source into destination space using header...', verbose)
# sct.run('isct_antsRegistration -d 3 -t Translation[0] -m MI[dest_pad.nii,src.nii,1,16] -c 0 -f 1 -s 0 -o
# [regAffine,src_regAffine.nii] -n BSpline[3]', verbose)
# if segmentation, also do it for seg
# initialize list of warping fields
warp_forward = []
warp_inverse = []
# initial warping is specified, update list of warping fields and skip step=0
if fname_initwarp:
sct.printv('\nSkip step=0 and replace with initial transformations: ',
param.verbose)
sct.printv(' ' + fname_initwarp, param.verbose)
# sct.copy(fname_initwarp, 'warp_forward_0.nii.gz')
warp_forward = [fname_initwarp]
start_step = 1
if fname_initwarpinv:
warp_inverse = [fname_initwarpinv]
else:
sct.printv(
'\nWARNING: No initial inverse warping field was specified, therefore the inverse warping field '
'will NOT be generated.', param.verbose, 'warning')
generate_warpinv = 0
else:
start_step = 0
# loop across registration steps
for i_step in range(start_step, len(paramreg.steps)):
sct.printv('\n--\nESTIMATE TRANSFORMATION FOR STEP #' + str(i_step),
param.verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = 'src.nii'
dest = 'dest_RPI.nii'
interp_step = 'spline'
elif paramreg.steps[str(i_step)].type == 'seg':
src = 'src_seg.nii'
dest = 'dest_seg_RPI.nii'
interp_step = 'nn'
elif paramreg.steps[str(i_step)].type == 'label':
src = 'src_label.nii'
dest = 'dest_label_RPI.nii'
interp_step = 'nn'
else:
# src = dest = interp_step = None
sct.printv('ERROR: Wrong image type.', 1, 'error')
# if step>0, apply warp_forward_concat to the src image to be used
if i_step > 0:
sct.printv('\nApply transformation from previous step',
param.verbose)
sct.run([
'sct_apply_transfo', '-i', src, '-d', dest, '-w',
','.join(warp_forward), '-o',
sct.add_suffix(src, '_reg'), '-x', interp_step
], verbose)
src = sct.add_suffix(src, '_reg')
# register src --> dest
warp_forward_out, warp_inverse_out = register(src, dest, paramreg,
param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations
sct.printv('\nConcatenate transformations...', verbose)
sct.run([
'sct_concat_transfo', '-w', ','.join(warp_forward), '-d', 'dest.nii',
'-o', 'warp_src2dest.nii.gz'
], verbose)
sct.run([
'sct_concat_transfo', '-w', ','.join(warp_inverse), '-d', 'src.nii',
'-o', 'warp_dest2src.nii.gz'
], verbose)
# Apply warping field to src data
sct.printv('\nApply transfo source --> dest...', verbose)
sct.run([
'sct_apply_transfo', '-i', 'src.nii', '-o', 'src_reg.nii', '-d',
'dest.nii', '-w', 'warp_src2dest.nii.gz', '-x', interp
], verbose)
sct.printv('\nApply transfo dest --> source...', verbose)
sct.run([
'sct_apply_transfo', '-i', 'dest.nii', '-o', 'dest_reg.nii', '-d',
'src.nii', '-w', 'warp_dest2src.nii.gz', '-x', interp
], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
# generate: src_reg
fname_src2dest = sct.generate_output_file(
os.path.join(path_tmp, "src_reg.nii"),
os.path.join(path_out, file_out + ext_out), verbose)
# generate: forward warping field
if fname_output_warp == '':
fname_output_warp = os.path.join(
path_out, 'warp_' + file_src + '2' + file_dest + '.nii.gz')
sct.generate_output_file(os.path.join(path_tmp, "warp_src2dest.nii.gz"),
fname_output_warp, verbose)
if generate_warpinv:
# generate: dest_reg
fname_dest2src = sct.generate_output_file(
os.path.join(path_tmp, "dest_reg.nii"),
os.path.join(path_out, file_out_inv + ext_dest), verbose)
# generate: inverse warping field
sct.generate_output_file(
os.path.join(path_tmp, "warp_dest2src.nii.gz"),
os.path.join(path_out,
'warp_' + file_dest + '2' + file_src + '.nii.gz'),
verbose)
# Delete temporary files
if remove_temp_files:
sct.printv('\nRemove temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv(
'\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's',
verbose)
if path_qc is not None:
if fname_dest_seg:
generate_qc(fname_src2dest,
fname_in2=fname_dest,
fname_seg=fname_dest_seg,
args=args,
path_qc=os.path.abspath(path_qc),
process='sct_register_multimodal')
else:
sct.printv(
'WARNING: Cannot generate QC because it requires destination segmentation.',
1, 'warning')
if generate_warpinv:
sct.display_viewer_syntax([fname_src, fname_dest2src], verbose=verbose)
sct.display_viewer_syntax([fname_dest, fname_src2dest], verbose=verbose)
def main(argv=None):
"""
Main function
:param argv:
:return:
"""
parser = get_parser()
arguments = parser.parse_args(argv)
verbose = arguments.v
set_global_loglevel(verbose=verbose)
input_filename = arguments.i
centerline_file = arguments.s
sc_straight = SpinalCordStraightener(input_filename, centerline_file)
if arguments.dest is not None:
sc_straight.use_straight_reference = True
sc_straight.centerline_reference_filename = str(arguments.dest)
if arguments.ldisc_input is not None:
if not sc_straight.use_straight_reference:
printv(
'Warning: discs position are not taken into account if reference is not provided.'
)
else:
sc_straight.discs_input_filename = str(arguments.ldisc_input)
sc_straight.precision = 4.0
if arguments.ldisc_dest is not None:
if not sc_straight.use_straight_reference:
printv(
'Warning: discs position are not taken into account if reference is not provided.'
)
else:
sc_straight.discs_ref_filename = str(arguments.ldisc_dest)
sc_straight.precision = 4.0
# Handling optional arguments
sc_straight.remove_temp_files = arguments.r
sc_straight.interpolation_warp = arguments.x
sc_straight.output_filename = arguments.o
sc_straight.path_output = arguments.ofolder
path_qc = arguments.qc
sc_straight.verbose = verbose
# if arguments.cpu_nb is not None:
# sc_straight.cpu_number = arguments.cpu-nb)
if arguments.disable_straight2curved:
sc_straight.straight2curved = False
if arguments.disable_curved2straight:
sc_straight.curved2straight = False
if arguments.speed_factor:
sc_straight.speed_factor = arguments.speed_factor
if arguments.xy_size:
sc_straight.xy_size = arguments.xy_size
sc_straight.param_centerline = ParamCenterline(
algo_fitting=arguments.centerline_algo,
smooth=arguments.centerline_smooth)
if arguments.param is not None:
params_user = arguments.param
# update registration parameters
for param in params_user:
param_split = param.split('=')
if param_split[0] == 'precision':
sc_straight.precision = float(param_split[1])
if param_split[0] == 'threshold_distance':
sc_straight.threshold_distance = float(param_split[1])
if param_split[0] == 'accuracy_results':
sc_straight.accuracy_results = int(param_split[1])
if param_split[0] == 'template_orientation':
sc_straight.template_orientation = int(param_split[1])
fname_straight = sc_straight.straighten()
printv("\nFinished! Elapsed time: {} s".format(sc_straight.elapsed_time),
verbose)
# Generate QC report
if path_qc is not None:
path_qc = os.path.abspath(path_qc)
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
generate_qc(fname_straight,
args=arguments,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process=os.path.basename(__file__.strip('.py')))
display_viewer_syntax([fname_straight], verbose=verbose)
def run_main():
init_sct()
parser = get_parser()
arguments = parser.parse_args(args=None if sys.argv[1:] else ['--help'])
# Input filename
fname_input_data = arguments.i
fname_data = os.path.abspath(fname_input_data)
# Method used
method = arguments.method
# Contrast type
contrast_type = arguments.c
if method == 'optic' and not contrast_type:
# Contrast must be
error = "ERROR: -c is a mandatory argument when using 'optic' method."
printv(error, type='error')
return
# Gap between slices
interslice_gap = arguments.gap
param_centerline = ParamCenterline(algo_fitting=arguments.centerline_algo,
smooth=arguments.centerline_smooth,
minmax=True)
# Output folder
if arguments.o is not None:
file_output = arguments.o
else:
path_data, file_data, ext_data = extract_fname(fname_data)
file_output = os.path.join(path_data, file_data + '_centerline')
verbose = int(arguments.v)
init_sct(log_level=verbose, update=True) # Update log level
if method == 'viewer':
# Manual labeling of cord centerline
im_labels = _call_viewer_centerline(Image(fname_data),
interslice_gap=interslice_gap)
elif method == 'fitseg':
im_labels = Image(fname_data)
elif method == 'optic':
# Automatic detection of cord centerline
im_labels = Image(fname_data)
param_centerline.algo_fitting = 'optic'
param_centerline.contrast = contrast_type
else:
printv(
"ERROR: The selected method is not available: {}. Please look at the help."
.format(method),
type='error')
return
# Extrapolate and regularize (or detect if optic) cord centerline
im_centerline, arr_centerline, _, _ = get_centerline(
im_labels, param=param_centerline, verbose=verbose)
# save centerline as nifti (discrete) and csv (continuous) files
im_centerline.save(file_output + '.nii.gz')
np.savetxt(file_output + '.csv', arr_centerline.transpose(), delimiter=",")
display_viewer_syntax([fname_input_data, file_output + '.nii.gz'],
colormaps=['gray', 'red'],
opacities=['', '1'])
path_qc = arguments.qc
qc_dataset = arguments.qc_dataset
qc_subject = arguments.qc_subject
# Generate QC report
if path_qc is not None:
generate_qc(fname_input_data,
fname_seg=file_output + '.nii.gz',
args=sys.argv[1:],
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_get_centerline')
display_viewer_syntax([fname_input_data, file_output + '.nii.gz'],
colormaps=['gray', 'red'],
opacities=['', '0.7'])
def main(args=None):
# initializations
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_data = arguments['-i']
fname_seg = arguments['-s']
if '-l' in arguments:
fname_landmarks = arguments['-l']
label_type = 'body'
elif '-ldisc' in arguments:
fname_landmarks = arguments['-ldisc']
label_type = 'disc'
else:
sct.printv('ERROR: Labels should be provided.', 1, 'error')
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = ''
param.path_qc = arguments.get("-qc", None)
path_template = arguments['-t']
contrast_template = arguments['-c']
ref = arguments['-ref']
param.remove_temp_files = int(arguments.get('-r'))
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
param.verbose = verbose # TODO: not clean, unify verbose or param.verbose in code, but not both
param.straighten_fitting = arguments['-straighten-fitting']
# if '-cpu-nb' in arguments:
# arg_cpu = ' -cpu-nb '+str(arguments['-cpu-nb'])
# else:
# arg_cpu = ''
# registration parameters
if '-param' in arguments:
# reset parameters but keep step=0 (might be overwritten if user specified step=0)
paramreg = ParamregMultiStep([step0])
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# add user parameters
for paramStep in arguments['-param']:
paramreg.addStep(paramStep)
else:
paramreg = ParamregMultiStep([step0, step1, step2])
# if ref=subject, initialize registration using different affine parameters
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# initialize other parameters
zsubsample = param.zsubsample
# retrieve template file names
file_template_vertebral_labeling = get_file_label(os.path.join(path_template, 'template'), 'vertebral labeling')
file_template = get_file_label(os.path.join(path_template, 'template'), contrast_template.upper() + '-weighted template')
file_template_seg = get_file_label(os.path.join(path_template, 'template'), 'spinal cord')
# start timer
start_time = time.time()
# get fname of the template + template objects
fname_template = os.path.join(path_template, 'template', file_template)
fname_template_vertebral_labeling = os.path.join(path_template, 'template', file_template_vertebral_labeling)
fname_template_seg = os.path.join(path_template, 'template', file_template_seg)
fname_template_disc_labeling = os.path.join(path_template, 'template', 'PAM50_label_disc.nii.gz')
# check file existence
# TODO: no need to do that!
sct.printv('\nCheck template files...')
sct.check_file_exist(fname_template, verbose)
sct.check_file_exist(fname_template_vertebral_labeling, verbose)
sct.check_file_exist(fname_template_seg, verbose)
path_data, file_data, ext_data = sct.extract_fname(fname_data)
# sct.printv(arguments)
sct.printv('\nCheck parameters:', verbose)
sct.printv(' Data: ' + fname_data, verbose)
sct.printv(' Landmarks: ' + fname_landmarks, verbose)
sct.printv(' Segmentation: ' + fname_seg, verbose)
sct.printv(' Path template: ' + path_template, verbose)
sct.printv(' Remove temp files: ' + str(param.remove_temp_files), verbose)
# check input labels
labels = check_labels(fname_landmarks, label_type=label_type)
vertebral_alignment = False
if len(labels) > 2 and label_type == 'disc':
vertebral_alignment = True
path_tmp = sct.tmp_create(basename="register_to_template", verbose=verbose)
# set temporary file names
ftmp_data = 'data.nii'
ftmp_seg = 'seg.nii.gz'
ftmp_label = 'label.nii.gz'
ftmp_template = 'template.nii'
ftmp_template_seg = 'template_seg.nii.gz'
ftmp_template_label = 'template_label.nii.gz'
# copy files to temporary folder
sct.printv('\nCopying input data to tmp folder and convert to nii...', verbose)
Image(fname_data).save(os.path.join(path_tmp, ftmp_data))
Image(fname_seg).save(os.path.join(path_tmp, ftmp_seg))
Image(fname_landmarks).save(os.path.join(path_tmp, ftmp_label))
Image(fname_template).save(os.path.join(path_tmp, ftmp_template))
Image(fname_template_seg).save(os.path.join(path_tmp, ftmp_template_seg))
Image(fname_template_vertebral_labeling).save(os.path.join(path_tmp, ftmp_template_label))
if label_type == 'disc':
Image(fname_template_disc_labeling).save(os.path.join(path_tmp, ftmp_template_label))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Generate labels from template vertebral labeling
if label_type == 'body':
sct.printv('\nGenerate labels from template vertebral labeling', verbose)
ftmp_template_label_, ftmp_template_label = ftmp_template_label, sct.add_suffix(ftmp_template_label, "_body")
sct_label_utils.main(args=['-i', ftmp_template_label_, '-vert-body', '0', '-o', ftmp_template_label])
# check if provided labels are available in the template
sct.printv('\nCheck if provided labels are available in the template', verbose)
image_label_template = Image(ftmp_template_label)
labels_template = image_label_template.getNonZeroCoordinates(sorting='value')
if labels[-1].value > labels_template[-1].value:
sct.printv('ERROR: Wrong landmarks input. Labels must have correspondence in template space. \nLabel max '
'provided: ' + str(labels[-1].value) + '\nLabel max from template: ' +
str(labels_template[-1].value), verbose, 'error')
# if only one label is present, force affine transformation to be Tx,Ty,Tz only (no scaling)
if len(labels) == 1:
paramreg.steps['0'].dof = 'Tx_Ty_Tz'
sct.printv('WARNING: Only one label is present. Forcing initial transformation to: ' + paramreg.steps['0'].dof,
1, 'warning')
# Project labels onto the spinal cord centerline because later, an affine transformation is estimated between the
# template's labels (centered in the cord) and the subject's labels (assumed to be centered in the cord).
# If labels are not centered, mis-registration errors are observed (see issue #1826)
ftmp_label = project_labels_on_spinalcord(ftmp_label, ftmp_seg)
# binarize segmentation (in case it has values below 0 caused by manual editing)
sct.printv('\nBinarize segmentation', verbose)
ftmp_seg_, ftmp_seg = ftmp_seg, sct.add_suffix(ftmp_seg, "_bin")
sct_maths.main(['-i', ftmp_seg_,
'-bin', '0.5',
'-o', ftmp_seg])
# Switch between modes: subject->template or template->subject
if ref == 'template':
# resample data to 1mm isotropic
sct.printv('\nResample data to 1mm isotropic...', verbose)
resample_file(ftmp_data, add_suffix(ftmp_data, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_data = add_suffix(ftmp_data, '_1mm')
resample_file(ftmp_seg, add_suffix(ftmp_seg, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_seg = add_suffix(ftmp_seg, '_1mm')
# N.B. resampling of labels is more complicated, because they are single-point labels, therefore resampling
# with nearest neighbour can make them disappear.
resample_labels(ftmp_label, ftmp_data, add_suffix(ftmp_label, '_1mm'))
ftmp_label = add_suffix(ftmp_label, '_1mm')
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
if vertebral_alignment:
# cropping the segmentation based on the label coverage to ensure good registration with vertebral alignment
# See https://github.com/neuropoly/spinalcordtoolbox/pull/1669 for details
image_labels = Image(ftmp_label)
coordinates_labels = image_labels.getNonZeroCoordinates(sorting='z')
nx, ny, nz, nt, px, py, pz, pt = image_labels.dim
offset_crop = 10.0 * pz # cropping the image 10 mm above and below the highest and lowest label
cropping_slices = [coordinates_labels[0].z - offset_crop, coordinates_labels[-1].z + offset_crop]
# make sure that the cropping slices do not extend outside of the slice range (issue #1811)
if cropping_slices[0] < 0:
cropping_slices[0] = 0
if cropping_slices[1] > nz:
cropping_slices[1] = nz
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, np.int32(np.round(cropping_slices))),))).save(ftmp_seg)
else:
# if we do not align the vertebral levels, we crop the segmentation from top to bottom
im_seg_rpi = Image(ftmp_seg_)
bottom = 0
for data in msct_image.SlicerOneAxis(im_seg_rpi, "IS"):
if (data != 0).any():
break
bottom += 1
top = im_seg_rpi.data.shape[2]
for data in msct_image.SlicerOneAxis(im_seg_rpi, "SI"):
if (data != 0).any():
break
top -= 1
msct_image.spatial_crop(im_seg_rpi, dict(((2, (bottom, top)),))).save(ftmp_seg)
# straighten segmentation
sct.printv('\nStraighten the spinal cord using centerline/segmentation...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
fn_warp_curve2straight = os.path.join(curdir, "warp_curve2straight.nii.gz")
fn_warp_straight2curve = os.path.join(curdir, "warp_straight2curve.nii.gz")
fn_straight_ref = os.path.join(curdir, "straight_ref.nii.gz")
cache_input_files=[ftmp_seg]
if vertebral_alignment:
cache_input_files += [
ftmp_template_seg,
ftmp_label,
ftmp_template_label,
]
cache_sig = sct.cache_signature(
input_files=cache_input_files,
)
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(fn_warp_curve2straight) and os.path.isfile(fn_warp_straight2curve) and os.path.isfile(fn_straight_ref):
sct.printv('Reusing existing warping field which seems to be valid', verbose, 'warning')
sct.copy(fn_warp_curve2straight, 'warp_curve2straight.nii.gz')
sct.copy(fn_warp_straight2curve, 'warp_straight2curve.nii.gz')
sct.copy(fn_straight_ref, 'straight_ref.nii.gz')
# apply straightening
sct.run(['sct_apply_transfo', '-i', ftmp_seg, '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', add_suffix(ftmp_seg, '_straight')])
else:
from spinalcordtoolbox.straightening import SpinalCordStraightener
sc_straight = SpinalCordStraightener(ftmp_seg, ftmp_seg)
sc_straight.algo_fitting = param.straighten_fitting
sc_straight.output_filename = add_suffix(ftmp_seg, '_straight')
sc_straight.path_output = './'
sc_straight.qc = '0'
sc_straight.remove_temp_files = param.remove_temp_files
sc_straight.verbose = verbose
if vertebral_alignment:
sc_straight.centerline_reference_filename = ftmp_template_seg
sc_straight.use_straight_reference = True
sc_straight.discs_input_filename = ftmp_label
sc_straight.discs_ref_filename = ftmp_template_label
sc_straight.straighten()
sct.cache_save(cachefile, cache_sig)
# N.B. DO NOT UPDATE VARIABLE ftmp_seg BECAUSE TEMPORARY USED LATER
# re-define warping field using non-cropped space (to avoid issue #367)
s, o = sct.run(['sct_concat_transfo', '-w', 'warp_straight2curve.nii.gz', '-d', ftmp_data, '-o', 'warp_straight2curve.nii.gz'])
if vertebral_alignment:
sct.copy('warp_curve2straight.nii.gz', 'warp_curve2straightAffine.nii.gz')
else:
# Label preparation:
# --------------------------------------------------------------------------------
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Dilating the input label so they can be straighten without losing them
sct.printv('\nDilating input labels using 3vox ball radius')
sct_maths.main(['-i', ftmp_label,
'-dilate', '3',
'-o', add_suffix(ftmp_label, '_dilate')])
ftmp_label = add_suffix(ftmp_label, '_dilate')
# Apply straightening to labels
sct.printv('\nApply straightening to labels...', verbose)
sct.run(['sct_apply_transfo', '-i', ftmp_label, '-o', add_suffix(ftmp_label, '_straight'), '-d', add_suffix(ftmp_seg, '_straight'), '-w', 'warp_curve2straight.nii.gz', '-x', 'nn'])
ftmp_label = add_suffix(ftmp_label, '_straight')
# Compute rigid transformation straight landmarks --> template landmarks
sct.printv('\nEstimate transformation for step #0...', verbose)
try:
register_landmarks(ftmp_label, ftmp_template_label, paramreg.steps['0'].dof,
fname_affine='straight2templateAffine.txt', verbose=verbose)
except RuntimeError:
raise('Input labels do not seem to be at the right place. Please check the position of the labels. '
'See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42')
# Concatenate transformations: curve --> straight --> affine
sct.printv('\nConcatenate transformations: curve --> straight --> affine...', verbose)
sct.run(['sct_concat_transfo', '-w', 'warp_curve2straight.nii.gz,straight2templateAffine.txt', '-d', 'template.nii', '-o', 'warp_curve2straightAffine.nii.gz'])
# Apply transformation
sct.printv('\nApply transformation...', verbose)
sct.run(['sct_apply_transfo', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_straightAffine'), '-d', ftmp_template, '-w', 'warp_curve2straightAffine.nii.gz'])
ftmp_data = add_suffix(ftmp_data, '_straightAffine')
sct.run(['sct_apply_transfo', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_straightAffine'), '-d', ftmp_template, '-w', 'warp_curve2straightAffine.nii.gz', '-x', 'linear'])
ftmp_seg = add_suffix(ftmp_seg, '_straightAffine')
"""
# Benjamin: Issue from Allan Martin, about the z=0 slice that is screwed up, caused by the affine transform.
# Solution found: remove slices below and above landmarks to avoid rotation effects
points_straight = []
for coord in landmark_template:
points_straight.append(coord.z)
min_point, max_point = int(np.round(np.min(points_straight))), int(np.round(np.max(points_straight)))
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_black')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (min_point,max_point)),))).save(ftmp_seg)
"""
# open segmentation
im = Image(ftmp_seg)
im_new = msct_image.empty_like(im)
# binarize
im_new.data = im.data > 0.5
# find min-max of anat2template (for subsequent cropping)
zmin_template, zmax_template = msct_image.find_zmin_zmax(im_new, threshold=0.5)
# save binarized segmentation
im_new.save(add_suffix(ftmp_seg, '_bin')) # unused?
# crop template in z-direction (for faster processing)
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nCrop data in template space (for faster processing)...', verbose)
ftmp_template_, ftmp_template = ftmp_template, add_suffix(ftmp_template, '_crop')
msct_image.spatial_crop(Image(ftmp_template_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template)
ftmp_template_seg_, ftmp_template_seg = ftmp_template_seg, add_suffix(ftmp_template_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_template_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template_seg)
ftmp_data_, ftmp_data = ftmp_data, add_suffix(ftmp_data, '_crop')
msct_image.spatial_crop(Image(ftmp_data_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_data)
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_seg)
# sub-sample in z-direction
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nSub-sample in z-direction (for faster processing)...', verbose)
sct.run(['sct_resample', '-i', ftmp_template, '-o', add_suffix(ftmp_template, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template = add_suffix(ftmp_template, '_sub')
sct.run(['sct_resample', '-i', ftmp_template_seg, '-o', add_suffix(ftmp_template_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template_seg = add_suffix(ftmp_template_seg, '_sub')
sct.run(['sct_resample', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_data = add_suffix(ftmp_data, '_sub')
sct.run(['sct_resample', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_seg = add_suffix(ftmp_seg, '_sub')
# Registration straight spinal cord to template
sct.printv('\nRegister straight spinal cord to template...', verbose)
# loop across registration steps
warp_forward = []
warp_inverse = []
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_data
dest = ftmp_template
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_seg
dest = ftmp_template_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog':
src_seg = ftmp_seg
dest_seg = ftmp_template_seg
# if step>1, apply warp_forward_concat to the src image to be used
if i_step > 1:
# apply transformation from previous step, to use as new src for registration
sct.run(['sct_apply_transfo', '-i', src, '-d', dest, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src = add_suffix(src, '_regStep' + str(i_step - 1))
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # also apply transformation to the seg
sct.run(['sct_apply_transfo', '-i', src_seg, '-d', dest_seg, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src_seg = add_suffix(src_seg, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # im_seg case
warp_forward_out, warp_inverse_out = register([src, src_seg], [dest, dest_seg], paramreg, param, str(i_step))
else:
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.append(warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: anat --> template...', verbose)
sct.run(['sct_concat_transfo', '-w', 'warp_curve2straightAffine.nii.gz,' + ','.join(warp_forward), '-d', 'template.nii', '-o', 'warp_anat2template.nii.gz'], verbose)
# sct.run('sct_concat_transfo -w warp_curve2straight.nii.gz,straight2templateAffine.txt,'+','.join(warp_forward)+' -d template.nii -o warp_anat2template.nii.gz', verbose)
sct.printv('\nConcatenate transformations: template --> anat...', verbose)
warp_inverse.reverse()
if vertebral_alignment:
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse) + ',warp_straight2curve.nii.gz', '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
else:
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse) + ',-straight2templateAffine.txt,warp_straight2curve.nii.gz', '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
# register template->subject
elif ref == 'subject':
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Add one label because at least 3 orthogonal labels are required to estimate an affine transformation. This
# new label is added at the level of the upper most label (lowest value), at 1cm to the right.
for i_file in [ftmp_label, ftmp_template_label]:
im_label = Image(i_file)
coord_label = im_label.getCoordinatesAveragedByValue() # N.B. landmarks are sorted by value
# Create new label
from copy import deepcopy
new_label = deepcopy(coord_label[0])
# move it 5mm to the left (orientation is RAS)
nx, ny, nz, nt, px, py, pz, pt = im_label.dim
new_label.x = np.round(coord_label[0].x + 5.0 / px)
# assign value 99
new_label.value = 99
# Add to existing image
im_label.data[int(new_label.x), int(new_label.y), int(new_label.z)] = new_label.value
# Overwrite label file
# im_label.absolutepath = 'label_rpi_modif.nii.gz'
im_label.save()
# Bring template to subject space using landmark-based transformation
sct.printv('\nEstimate transformation for step #0...', verbose)
warp_forward = ['template2subjectAffine.txt']
warp_inverse = ['-template2subjectAffine.txt']
try:
register_landmarks(ftmp_template_label, ftmp_label, paramreg.steps['0'].dof, fname_affine=warp_forward[0], verbose=verbose, path_qc="./")
except Exception:
sct.printv('ERROR: input labels do not seem to be at the right place. Please check the position of the labels. See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42', verbose=verbose, type='error')
# loop across registration steps
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_template
dest = ftmp_data
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_template_seg
dest = ftmp_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
# apply transformation from previous step, to use as new src for registration
sct.run(['sct_apply_transfo', '-i', src, '-d', dest, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src = add_suffix(src, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: template --> subject...', verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_forward), '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
sct.printv('\nConcatenate transformations: subject --> template...', verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse), '-d', 'template.nii', '-o', 'warp_anat2template.nii.gz'], verbose)
# Apply warping fields to anat and template
sct.run(['sct_apply_transfo', '-i', 'template.nii', '-o', 'template2anat.nii.gz', '-d', 'data.nii', '-w', 'warp_template2anat.nii.gz', '-crop', '1'], verbose)
sct.run(['sct_apply_transfo', '-i', 'data.nii', '-o', 'anat2template.nii.gz', '-d', 'template.nii', '-w', 'warp_anat2template.nii.gz', '-crop', '1'], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
fname_template2anat = os.path.join(path_output, 'template2anat' + ext_data)
fname_anat2template = os.path.join(path_output, 'anat2template' + ext_data)
sct.generate_output_file(os.path.join(path_tmp, "warp_template2anat.nii.gz"), os.path.join(path_output, "warp_template2anat.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_anat2template.nii.gz"), os.path.join(path_output, "warp_anat2template.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "template2anat.nii.gz"), fname_template2anat, verbose)
sct.generate_output_file(os.path.join(path_tmp, "anat2template.nii.gz"), fname_anat2template, verbose)
if ref == 'template':
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose)
# Delete temporary files
if param.remove_temp_files:
sct.printv('\nDelete temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if param.path_qc is not None:
generate_qc(fname_data, fname_in2=fname_template2anat, fname_seg=fname_seg, args=args,
path_qc=os.path.abspath(param.path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_to_template')
sct.display_viewer_syntax([fname_data, fname_template2anat], verbose=verbose)
sct.display_viewer_syntax([fname_template, fname_anat2template], verbose=verbose)
def main(args=None):
if args is None:
args = sys.argv[1:]
# initialize parameters
param = Param()
# Initialization
fname_output = ''
path_out = ''
fname_src_seg = ''
fname_dest_seg = ''
fname_src_label = ''
fname_dest_label = ''
generate_warpinv = 1
start_time = time.time()
# get default registration parameters
# step1 = Paramreg(step='1', type='im', algo='syn', metric='MI', iter='5', shrink='1', smooth='0', gradStep='0.5')
step0 = Paramreg(step='0', type='im', algo='syn', metric='MI', iter='0', shrink='1', smooth='0', gradStep='0.5',
slicewise='0', dof='Tx_Ty_Tz_Rx_Ry_Rz') # only used to put src into dest space
step1 = Paramreg(step='1', type='im')
paramregmulti = ParamregMultiStep([step0, step1])
parser = get_parser(paramregmulti=paramregmulti)
arguments = parser.parse(args)
# get arguments
fname_src = arguments['-i']
fname_dest = arguments['-d']
if '-iseg' in arguments:
fname_src_seg = arguments['-iseg']
if '-dseg' in arguments:
fname_dest_seg = arguments['-dseg']
if '-ilabel' in arguments:
fname_src_label = arguments['-ilabel']
if '-dlabel' in arguments:
fname_dest_label = arguments['-dlabel']
if '-o' in arguments:
fname_output = arguments['-o']
if '-ofolder' in arguments:
path_out = arguments['-ofolder']
if '-owarp' in arguments:
fname_output_warp = arguments['-owarp']
else:
fname_output_warp = ''
if '-initwarp' in arguments:
fname_initwarp = os.path.abspath(arguments['-initwarp'])
else:
fname_initwarp = ''
if '-initwarpinv' in arguments:
fname_initwarpinv = os.path.abspath(arguments['-initwarpinv'])
else:
fname_initwarpinv = ''
if '-m' in arguments:
fname_mask = arguments['-m']
else:
fname_mask = ''
padding = arguments['-z']
if "-param" in arguments:
paramregmulti_user = arguments['-param']
# update registration parameters
for paramStep in paramregmulti_user:
paramregmulti.addStep(paramStep)
path_qc = arguments.get("-qc", None)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
identity = int(arguments['-identity'])
interp = arguments['-x']
remove_temp_files = int(arguments['-r'])
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
# sct.printv(arguments)
sct.printv('\nInput parameters:')
sct.printv(' Source .............. ' + fname_src)
sct.printv(' Destination ......... ' + fname_dest)
sct.printv(' Init transfo ........ ' + fname_initwarp)
sct.printv(' Mask ................ ' + fname_mask)
sct.printv(' Output name ......... ' + fname_output)
# sct.printv(' Algorithm ........... '+paramregmulti.algo)
# sct.printv(' Number of iterations '+paramregmulti.iter)
# sct.printv(' Metric .............. '+paramregmulti.metric)
sct.printv(' Remove temp files ... ' + str(remove_temp_files))
sct.printv(' Verbose ............. ' + str(verbose))
# update param
param.verbose = verbose
param.padding = padding
param.fname_mask = fname_mask
param.remove_temp_files = remove_temp_files
# Get if input is 3D
sct.printv('\nCheck if input data are 3D...', verbose)
sct.check_dim(fname_src, dim_lst=[3])
sct.check_dim(fname_dest, dim_lst=[3])
# Check if user selected type=seg, but did not input segmentation data
if 'paramregmulti_user' in locals():
if True in ['type=seg' in paramregmulti_user[i] for i in range(len(paramregmulti_user))]:
if fname_src_seg == '' or fname_dest_seg == '':
sct.printv('\nERROR: if you select type=seg you must specify -iseg and -dseg flags.\n', 1, 'error')
# Put source into destination space using header (no estimation -- purely based on header)
# TODO: Check if necessary to do that
# TODO: use that as step=0
# sct.printv('\nPut source into destination space using header...', verbose)
# sct.run('isct_antsRegistration -d 3 -t Translation[0] -m MI[dest_pad.nii,src.nii,1,16] -c 0 -f 1 -s 0 -o
# [regAffine,src_regAffine.nii] -n BSpline[3]', verbose)
# if segmentation, also do it for seg
fname_src2dest, fname_dest2src, _, _ = \
register_wrapper(fname_src, fname_dest, param, paramregmulti, fname_src_seg=fname_src_seg,
fname_dest_seg=fname_dest_seg, fname_src_label=fname_src_label,
fname_dest_label=fname_dest_label, fname_mask=fname_mask, fname_initwarp=fname_initwarp,
fname_initwarpinv=fname_initwarpinv, identity=identity, interp=interp,
fname_output=fname_output,
fname_output_warp=fname_output_warp,
path_out=path_out)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
if path_qc is not None:
if fname_dest_seg:
generate_qc(fname_src2dest, fname_in2=fname_dest, fname_seg=fname_dest_seg, args=args,
path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_multimodal')
else:
sct.printv('WARNING: Cannot generate QC because it requires destination segmentation.', 1, 'warning')
if generate_warpinv:
sct.display_viewer_syntax([fname_src, fname_dest2src], verbose=verbose)
sct.display_viewer_syntax([fname_dest, fname_src2dest], verbose=verbose)
def main(args=None):
# initializations
initz = ''
initcenter = ''
fname_initlabel = ''
file_labelz = 'labelz.nii.gz'
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_in = os.path.abspath(arguments["-i"])
fname_seg = os.path.abspath(arguments['-s'])
contrast = arguments['-c']
path_template = os.path.abspath(arguments['-t'])
scale_dist = arguments['-scale-dist']
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = os.curdir
param.path_qc = arguments.get("-qc", None)
if '-discfile' in arguments:
fname_disc = os.path.abspath(arguments['-discfile'])
else:
fname_disc = None
if '-initz' in arguments:
initz = arguments['-initz']
if '-initcenter' in arguments:
initcenter = arguments['-initcenter']
# if user provided text file, parse and overwrite arguments
if '-initfile' in arguments:
file = open(arguments['-initfile'], 'r')
initfile = ' ' + file.read().replace('\n', '')
arg_initfile = initfile.split(' ')
for idx_arg, arg in enumerate(arg_initfile):
if arg == '-initz':
initz = [int(x) for x in arg_initfile[idx_arg + 1].split(',')]
if arg == '-initcenter':
initcenter = int(arg_initfile[idx_arg + 1])
if '-initlabel' in arguments:
# get absolute path of label
fname_initlabel = os.path.abspath(arguments['-initlabel'])
if '-param' in arguments:
param.update(arguments['-param'][0])
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
remove_temp_files = int(arguments['-r'])
denoise = int(arguments['-denoise'])
laplacian = int(arguments['-laplacian'])
path_tmp = sct.tmp_create(basename="label_vertebrae", verbose=verbose)
# Copying input data to tmp folder
sct.printv('\nCopying input data to tmp folder...', verbose)
Image(fname_in).save(os.path.join(path_tmp, "data.nii"))
Image(fname_seg).save(os.path.join(path_tmp, "segmentation.nii"))
# Go go temp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Straighten spinal cord
sct.printv('\nStraighten spinal cord...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
cache_sig = sct.cache_signature(input_files=[fname_in, fname_seg], )
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(
os.path.join(
curdir, "warp_curve2straight.nii.gz")) and os.path.isfile(
os.path.join(
curdir,
"warp_straight2curve.nii.gz")) and os.path.isfile(
os.path.join(curdir, "straight_ref.nii.gz")):
# if they exist, copy them into current folder
sct.printv('Reusing existing warping field which seems to be valid',
verbose, 'warning')
sct.copy(os.path.join(curdir, "warp_curve2straight.nii.gz"),
'warp_curve2straight.nii.gz')
sct.copy(os.path.join(curdir, "warp_straight2curve.nii.gz"),
'warp_straight2curve.nii.gz')
sct.copy(os.path.join(curdir, "straight_ref.nii.gz"),
'straight_ref.nii.gz')
# apply straightening
s, o = sct.run([
'sct_apply_transfo', '-i', 'data.nii', '-w',
'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o',
'data_straight.nii'
])
else:
sct_straighten_spinalcord.main(args=[
'-i',
'data.nii',
'-s',
'segmentation.nii',
'-r',
str(remove_temp_files),
'-v',
str(verbose),
])
sct.cache_save(cachefile, cache_sig)
# resample to 0.5mm isotropic to match template resolution
sct.printv('\nResample to 0.5mm isotropic...', verbose)
s, o = sct.run([
'sct_resample', '-i', 'data_straight.nii', '-mm', '0.5x0.5x0.5', '-x',
'linear', '-o', 'data_straightr.nii'
],
verbose=verbose)
# Apply straightening to segmentation
# N.B. Output is RPI
sct.printv('\nApply straightening to segmentation...', verbose)
sct.run(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation.nii', 'data_straightr.nii',
'warp_curve2straight.nii.gz', 'segmentation_straight.nii', 'Linear'),
verbose=verbose,
is_sct_binary=True,
)
# Threshold segmentation at 0.5
sct.run([
'sct_maths', '-i', 'segmentation_straight.nii', '-thr', '0.5', '-o',
'segmentation_straight.nii'
], verbose)
# If disc label file is provided, label vertebrae using that file instead of automatically
if fname_disc:
# Apply straightening to disc-label
sct.printv('\nApply straightening to disc labels...', verbose)
sct.run(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
(fname_disc, 'data_straightr.nii', 'warp_curve2straight.nii.gz',
'labeldisc_straight.nii.gz', 'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
label_vert('segmentation_straight.nii',
'labeldisc_straight.nii.gz',
verbose=1)
else:
# create label to identify disc
sct.printv('\nCreate label to identify disc...', verbose)
fname_labelz = os.path.join(path_tmp, file_labelz)
if initz or initcenter:
if initcenter:
# find z centered in FOV
nii = Image('segmentation.nii').change_orientation("RPI")
nx, ny, nz, nt, px, py, pz, pt = nii.dim # Get dimensions
z_center = int(np.round(nz / 2)) # get z_center
initz = [z_center, initcenter]
# create single label and output as labels.nii.gz
label = ProcessLabels(
'segmentation.nii',
fname_output='tmp.labelz.nii.gz',
coordinates=['{},{}'.format(initz[0], initz[1])])
im_label = label.process('create-seg')
im_label.data = dilate(
im_label.data, 3,
'ball') # TODO: create a dilation method specific to labels,
# which does not apply a convolution across all voxels (highly inneficient)
im_label.save(fname_labelz)
elif fname_initlabel:
Image(fname_initlabel).save(fname_labelz)
else:
# automatically finds C2-C3 disc
im_data = Image('data.nii')
im_seg = Image('segmentation.nii')
if not remove_temp_files: # because verbose is here also used for keeping temp files
verbose_detect_c2c3 = 2
else:
verbose_detect_c2c3 = 0
im_label_c2c3 = detect_c2c3(im_data,
im_seg,
contrast,
verbose=verbose_detect_c2c3)
ind_label = np.where(im_label_c2c3.data)
if not np.size(ind_label) == 0:
im_label_c2c3.data[ind_label] = 3
else:
sct.printv(
'Automatic C2-C3 detection failed. Please provide manual label with sct_label_utils',
1, 'error')
sys.exit()
im_label_c2c3.save(fname_labelz)
# dilate label so it is not lost when applying warping
dilate(Image(fname_labelz), 3, 'ball').save(fname_labelz)
# Apply straightening to z-label
sct.printv('\nAnd apply straightening to label...', verbose)
sct.run(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
(file_labelz, 'data_straightr.nii', 'warp_curve2straight.nii.gz',
'labelz_straight.nii.gz', 'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
# get z value and disk value to initialize labeling
sct.printv('\nGet z and disc values from straight label...', verbose)
init_disc = get_z_and_disc_values_from_label('labelz_straight.nii.gz')
sct.printv('.. ' + str(init_disc), verbose)
# denoise data
if denoise:
sct.printv('\nDenoise data...', verbose)
sct.run([
'sct_maths', '-i', 'data_straightr.nii', '-denoise', 'h=0.05',
'-o', 'data_straightr.nii'
], verbose)
# apply laplacian filtering
if laplacian:
sct.printv('\nApply Laplacian filter...', verbose)
sct.run([
'sct_maths', '-i', 'data_straightr.nii', '-laplacian', '1',
'-o', 'data_straightr.nii'
], verbose)
# detect vertebral levels on straight spinal cord
init_disc[1] = init_disc[1] - 1
vertebral_detection('data_straightr.nii',
'segmentation_straight.nii',
contrast,
param,
init_disc=init_disc,
verbose=verbose,
path_template=path_template,
path_output=path_output,
scale_dist=scale_dist)
# un-straighten labeled spinal cord
sct.printv('\nUn-straighten labeling...', verbose)
sct.run(
'isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' %
('segmentation_straight_labeled.nii', 'segmentation.nii',
'warp_straight2curve.nii.gz', 'segmentation_labeled.nii',
'NearestNeighbor'),
verbose=verbose,
is_sct_binary=True,
)
# Clean labeled segmentation
sct.printv(
'\nClean labeled segmentation (correct interpolation errors)...',
verbose)
clean_labeled_segmentation('segmentation_labeled.nii', 'segmentation.nii',
'segmentation_labeled.nii')
# label discs
sct.printv('\nLabel discs...', verbose)
label_discs('segmentation_labeled.nii', verbose=verbose)
# come back
os.chdir(curdir)
# Generate output files
path_seg, file_seg, ext_seg = sct.extract_fname(fname_seg)
fname_seg_labeled = os.path.join(path_output,
file_seg + '_labeled' + ext_seg)
sct.printv('\nGenerate output files...', verbose)
sct.generate_output_file(
os.path.join(path_tmp, "segmentation_labeled.nii"), fname_seg_labeled)
sct.generate_output_file(
os.path.join(path_tmp, "segmentation_labeled_disc.nii"),
os.path.join(path_output, file_seg + '_labeled_discs' + ext_seg))
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(
os.path.join(path_tmp, "warp_curve2straight.nii.gz"),
os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(
os.path.join(path_tmp, "warp_straight2curve.nii.gz"),
os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"),
os.path.join(path_output, "straight_ref.nii.gz"),
verbose)
# Remove temporary files
if remove_temp_files == 1:
sct.printv('\nRemove temporary files...', verbose)
sct.rmtree(path_tmp)
# Generate QC report
if param.path_qc is not None:
path_qc = os.path.abspath(param.path_qc)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
labeled_seg_file = os.path.join(path_output,
file_seg + '_labeled' + ext_seg)
generate_qc(fname_in,
fname_seg=labeled_seg_file,
args=args,
path_qc=os.path.abspath(path_qc),
dataset=qc_dataset,
subject=qc_subject,
process='sct_label_vertebrae')
sct.display_viewer_syntax([fname_in, fname_seg_labeled],
colormaps=['', 'subcortical'],
opacities=['1', '0.5'])