def _preprocess_segment(fname_t2, fname_t2_seg, contrast_test, dim_3=False):
tmp_folder = sct.TempFolder()
tmp_folder_path = tmp_folder.get_path()
tmp_folder.chdir()
img = Image(fname_t2)
gt = Image(fname_t2_seg)
fname_t2_RPI, fname_t2_seg_RPI = 'img_RPI.nii.gz', 'seg_RPI.nii.gz'
img.change_orientation('RPI').save(fname_t2_RPI)
gt.change_orientation('RPI').save(fname_t2_seg_RPI)
input_resolution = gt.dim[4:7]
del img, gt
fname_res, fname_ctr, _ = deepseg_sc.find_centerline(algo='svm',
image_fname=fname_t2_RPI,
contrast_type=contrast_test,
brain_bool=False,
folder_output=tmp_folder_path,
remove_temp_files=1,
centerline_fname=None)
fname_t2_seg_RPI_res = 'seg_RPI_res.nii.gz'
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resample_file(fname_t2_seg_RPI, fname_t2_seg_RPI_res, new_resolution, 'mm', 'linear', verbose=0)
img, ctr, gt = Image(fname_res), Image(fname_ctr), Image(fname_t2_seg_RPI_res)
_, _, _, img = deepseg_sc.crop_image_around_centerline(im_in=img,
ctr_in=ctr,
crop_size=64)
_, _, _, gt = deepseg_sc.crop_image_around_centerline(im_in=gt,
ctr_in=ctr,
crop_size=64)
del ctr
img = deepseg_sc.apply_intensity_normalization(im_in=img)
if dim_3: # If 3D kernels
fname_t2_RPI_res_crop, fname_t2_seg_RPI_res_crop = 'img_RPI_res_crop.nii.gz', 'seg_RPI_res_crop.nii.gz'
img.save(fname_t2_RPI_res_crop)
gt.save(fname_t2_seg_RPI_res_crop)
del img, gt
fname_t2_RPI_res_crop_res = 'img_RPI_res_crop_res.nii.gz'
fname_t2_seg_RPI_res_crop_res = 'seg_RPI_res_crop_res.nii.gz'
resample_file(fname_t2_RPI_res_crop, fname_t2_RPI_res_crop_res, new_resolution, 'mm', 'linear', verbose=0)
resample_file(fname_t2_seg_RPI_res_crop, fname_t2_seg_RPI_res_crop_res, new_resolution, 'mm', 'linear', verbose=0)
img, gt = Image(fname_t2_RPI_res_crop_res), Image(fname_t2_seg_RPI_res_crop_res)
tmp_folder.chdir_undo()
tmp_folder.cleanup()
return img, gt
def _preprocess_segment(fname_t2, fname_t2_seg, contrast_test, dim_3=False):
tmp_folder = sct.TempFolder()
tmp_folder_path = tmp_folder.get_path()
tmp_folder.chdir()
img = Image(fname_t2)
gt = Image(fname_t2_seg)
fname_t2_RPI, fname_t2_seg_RPI = 'img_RPI.nii.gz', 'seg_RPI.nii.gz'
img.change_orientation('RPI').save(fname_t2_RPI)
gt.change_orientation('RPI').save(fname_t2_seg_RPI)
input_resolution = gt.dim[4:7]
del img, gt
fname_res, fname_ctr = deepseg_sc.find_centerline(algo='svm',
image_fname=fname_t2_RPI,
contrast_type=contrast_test,
brain_bool=False,
folder_output=tmp_folder_path,
remove_temp_files=1,
centerline_fname=None)
fname_t2_seg_RPI_res = 'seg_RPI_res.nii.gz'
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resample_file(fname_t2_seg_RPI, fname_t2_seg_RPI_res, new_resolution, 'mm', 'linear', verbose=0)
img, ctr, gt = Image(fname_res), Image(fname_ctr), Image(fname_t2_seg_RPI_res)
_, _, _, img = deepseg_sc.crop_image_around_centerline(im_in=img,
ctr_in=ctr,
crop_size=64)
_, _, _, gt = deepseg_sc.crop_image_around_centerline(im_in=gt,
ctr_in=ctr,
crop_size=64)
del ctr
img = deepseg_sc.apply_intensity_normalization(im_in=img)
if dim_3: # If 3D kernels
fname_t2_RPI_res_crop, fname_t2_seg_RPI_res_crop = 'img_RPI_res_crop.nii.gz', 'seg_RPI_res_crop.nii.gz'
img.save(fname_t2_RPI_res_crop)
gt.save(fname_t2_seg_RPI_res_crop)
del img, gt
fname_t2_RPI_res_crop_res = 'img_RPI_res_crop_res.nii.gz'
fname_t2_seg_RPI_res_crop_res = 'seg_RPI_res_crop_res.nii.gz'
resample_file(fname_t2_RPI_res_crop, fname_t2_RPI_res_crop_res, new_resolution, 'mm', 'linear', verbose=0)
resample_file(fname_t2_seg_RPI_res_crop, fname_t2_seg_RPI_res_crop_res, new_resolution, 'mm', 'linear', verbose=0)
img, gt = Image(fname_t2_RPI_res_crop_res), Image(fname_t2_seg_RPI_res_crop_res)
tmp_folder.chdir_undo()
tmp_folder.cleanup()
return img, gt
def deep_segmentation_spinalcord(im_image,
contrast_type,
ctr_algo='cnn',
ctr_file=None,
brain_bool=True,
kernel_size='2d',
remove_temp_files=1,
verbose=1):
"""Pipeline"""
# create temporary folder with intermediate results
tmp_folder = sct.TempFolder(verbose=verbose)
tmp_folder_path = tmp_folder.get_path()
if ctr_algo == 'file': # if the ctr_file is provided
tmp_folder.copy_from(ctr_file)
file_ctr = os.path.basename(ctr_file)
else:
file_ctr = None
tmp_folder.chdir()
# re-orient image to RPI
logger.info("Reorient the image to RPI, if necessary...")
original_orientation = im_image.orientation
fname_orient = 'image_in_RPI.nii'
im_image.change_orientation('RPI').save(fname_orient)
input_resolution = im_image.dim[4:7]
# find the spinal cord centerline - execute OptiC binary
logger.info("Finding the spinal cord centerline...")
fname_res, centerline_filename = find_centerline(
algo=ctr_algo,
image_fname=fname_orient,
contrast_type=contrast_type,
brain_bool=brain_bool,
folder_output=tmp_folder_path,
remove_temp_files=remove_temp_files,
centerline_fname=file_ctr)
im_nii, ctr_nii = Image(fname_res), Image(centerline_filename)
# crop image around the spinal cord centerline
logger.info("Cropping the image around the spinal cord...")
crop_size = 96 if (kernel_size == '3d' and contrast_type == 't2s') else 64
X_CROP_LST, Y_CROP_LST, Z_CROP_LST, im_crop_nii = crop_image_around_centerline(
im_in=im_nii, ctr_in=ctr_nii, crop_size=crop_size)
del ctr_nii
# normalize the intensity of the images
logger.info("Normalizing the intensity...")
im_norm_in = apply_intensity_normalization(im_in=im_crop_nii)
del im_crop_nii
if kernel_size == '2d':
# segment data using 2D convolutions
logger.info(
"Segmenting the spinal cord using deep learning on 2D patches...")
segmentation_model_fname = \
os.path.join(sct.__sct_dir__, 'data', 'deepseg_sc_models', '{}_sc.h5'.format(contrast_type))
seg_crop_data = segment_2d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
input_size=(crop_size, crop_size),
im_in=im_norm_in)
elif kernel_size == '3d':
# segment data using 3D convolutions
logger.info(
"Segmenting the spinal cord using deep learning on 3D patches...")
segmentation_model_fname = \
os.path.join(sct.__sct_dir__, 'data', 'deepseg_sc_models', '{}_sc_3D.h5'.format(contrast_type))
seg_crop_data = segment_3d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
im_in=im_norm_in)
del im_norm_in
# reconstruct the segmentation from the crop data
logger.info("Reassembling the image...")
seg_uncrop_nii = uncrop_image(ref_in=im_nii,
data_crop=seg_crop_data,
x_crop_lst=X_CROP_LST,
y_crop_lst=Y_CROP_LST,
z_crop_lst=Z_CROP_LST)
fname_res_seg = sct.add_suffix(fname_res, '_seg')
seg_uncrop_nii.save(fname_res_seg)
del seg_crop_data
# resample to initial resolution
logger.info(
"Resampling the segmentation to the original image resolution...")
initial_resolution = 'x'.join([
str(input_resolution[0]),
str(input_resolution[1]),
str(input_resolution[2])
])
fname_res_seg_downsamp = sct.add_suffix(fname_res_seg, '_downsamp')
resampling.resample_file(fname_res_seg,
fname_res_seg_downsamp,
initial_resolution,
'mm',
'linear',
verbose=0)
im_image_res_seg_downsamp = Image(fname_res_seg_downsamp)
if ctr_algo == 'viewer': # resample and reorient the viewer labels
fname_res_labels = sct.add_suffix(fname_orient, '_labels-centerline')
resampling.resample_file(fname_res_labels,
fname_res_labels,
initial_resolution,
'mm',
'linear',
verbose=0)
im_image_res_labels_downsamp = Image(
fname_res_labels).change_orientation(original_orientation)
else:
im_image_res_labels_downsamp = None
if verbose == 2:
fname_res_ctr = sct.add_suffix(fname_orient, '_ctr')
resampling.resample_file(fname_res_ctr,
fname_res_ctr,
initial_resolution,
'mm',
'linear',
verbose=0)
im_image_res_ctr_downsamp = Image(fname_res_ctr).change_orientation(
original_orientation)
else:
im_image_res_ctr_downsamp = None
# binarize the resampled image to remove interpolation effects
logger.info(
"Binarizing the segmentation to avoid interpolation effects...")
thr = 0.0001 if contrast_type in ['t1', 'dwi'] else 0.5
# TODO: optimize speed --> np.where is slow
im_image_res_seg_downsamp.data[np.where(
im_image_res_seg_downsamp.data >= thr)] = 1
im_image_res_seg_downsamp.data[np.where(
im_image_res_seg_downsamp.data < thr)] = 0
# post processing step to z_regularized
im_image_res_seg_downsamp_postproc = post_processing_volume_wise(
im_in=im_image_res_seg_downsamp)
tmp_folder.chdir_undo()
# remove temporary files
if remove_temp_files:
logger.info("Remove temporary files...")
tmp_folder.cleanup()
# reorient to initial orientation
return im_image_res_seg_downsamp_postproc.change_orientation(original_orientation), \
im_nii, \
seg_uncrop_nii.change_orientation('RPI'), \
im_image_res_labels_downsamp, \
im_image_res_ctr_downsamp
def find_centerline(algo, image_fname, contrast_type, brain_bool,
folder_output, remove_temp_files, centerline_fname):
"""
Assumes RPI orientation
:param algo:
:param image_fname:
:param contrast_type:
:param brain_bool:
:param folder_output:
:param remove_temp_files:
:param centerline_fname:
:return:
"""
# TODO: remove unnecessary i/o
if Image(image_fname).dim[2] == 1: # isct_spine_detect requires nz > 1
im_concat = concat_data([image_fname, image_fname], dim=2)
im_concat.save(sct.add_suffix(image_fname, '_concat'))
image_fname = sct.add_suffix(image_fname, '_concat')
bool_2d = True
else:
bool_2d = False
# TODO: maybe change 'svm' for 'optic', because this is how we call it in sct_get_centerline
if algo == 'svm':
# run optic on a heatmap computed by a trained SVM+HoG algorithm
# optic_models_fname = os.path.join(path_sct, 'data', 'optic_models', '{}_model'.format(contrast_type))
# # TODO: replace with get_centerline(method=optic)
img_ctl, arr_ctl, _ = get_centerline(Image(image_fname),
algo_fitting='optic',
contrast=contrast_type)
centerline_filename = sct.add_suffix(image_fname, "_ctr")
img_ctl.save(centerline_filename)
elif algo == 'cnn':
# CNN parameters
dct_patch_ctr = {
't2': {
'size': (80, 80),
'mean': 51.1417,
'std': 57.4408
},
't2s': {
'size': (80, 80),
'mean': 68.8591,
'std': 71.4659
},
't1': {
'size': (80, 80),
'mean': 55.7359,
'std': 64.3149
},
'dwi': {
'size': (80, 80),
'mean': 55.744,
'std': 45.003
}
}
dct_params_ctr = {
't2': {
'features': 16,
'dilation_layers': 2
},
't2s': {
'features': 8,
'dilation_layers': 3
},
't1': {
'features': 24,
'dilation_layers': 3
},
'dwi': {
'features': 8,
'dilation_layers': 2
}
}
# load model
ctr_model_fname = os.path.join(sct.__sct_dir__, 'data',
'deepseg_sc_models',
'{}_ctr.h5'.format(contrast_type))
ctr_model = nn_architecture_ctr(
height=dct_patch_ctr[contrast_type]['size'][0],
width=dct_patch_ctr[contrast_type]['size'][1],
channels=1,
classes=1,
features=dct_params_ctr[contrast_type]['features'],
depth=2,
temperature=1.0,
padding='same',
batchnorm=True,
dropout=0.0,
dilation_layers=dct_params_ctr[contrast_type]['dilation_layers'])
ctr_model.load_weights(ctr_model_fname)
logger.info("Resample the image to 0.5x0.5 mm in-plane resolution...")
fname_res = sct.add_suffix(image_fname, '_resampled')
input_resolution = Image(image_fname).dim[4:7]
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(image_fname,
fname_res,
new_resolution,
'mm',
'linear',
verbose=0)
# compute the heatmap
fname_heatmap = sct.add_suffix(image_fname, "_heatmap")
img_filename = ''.join(sct.extract_fname(fname_heatmap)[:2])
fname_heatmap_nii = img_filename + '.nii'
z_max = heatmap(filename_in=fname_res,
filename_out=fname_heatmap_nii,
model=ctr_model,
patch_shape=dct_patch_ctr[contrast_type]['size'],
mean_train=dct_patch_ctr[contrast_type]['mean'],
std_train=dct_patch_ctr[contrast_type]['std'],
brain_bool=brain_bool)
# run optic on the heatmap
centerline_filename = sct.add_suffix(fname_heatmap, "_ctr")
heatmap2optic(fname_heatmap=fname_heatmap_nii,
lambda_value=7 if contrast_type == 't2s' else 1,
fname_out=centerline_filename,
z_max=z_max if brain_bool else None)
elif algo == 'viewer':
im_labels = _call_viewer_centerline(Image(image_fname))
im_centerline, arr_centerline, _ = get_centerline(im_labels)
centerline_filename = sct.add_suffix(image_fname, "_ctr")
labels_filename = sct.add_suffix(image_fname, "_labels-centerline")
im_centerline.save(centerline_filename)
im_labels.save(labels_filename)
elif algo == 'file':
centerline_filename = sct.add_suffix(image_fname, "_ctr")
# Re-orient the manual centerline
Image(centerline_fname).change_orientation('RPI').save(
centerline_filename)
else:
logger.error(
'The parameter "-centerline" is incorrect. Please try again.')
sys.exit(1)
if algo != 'cnn':
logger.info("Resample the image to 0.5x0.5 mm in-plane resolution...")
fname_res = sct.add_suffix(image_fname, '_resampled')
input_resolution = Image(image_fname).dim[4:7]
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(image_fname,
fname_res,
new_resolution,
'mm',
'linear',
verbose=0)
resampling.resample_file(centerline_filename,
centerline_filename,
new_resolution,
'mm',
'linear',
verbose=0)
if bool_2d:
im_split_lst = split_data(Image(centerline_filename), dim=2)
im_split_lst[0].save(centerline_filename)
return fname_res, centerline_filename
def main(args=None):
# initializations
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_data = arguments['-i']
fname_seg = arguments['-s']
if '-l' in arguments:
fname_landmarks = arguments['-l']
label_type = 'body'
elif '-ldisc' in arguments:
fname_landmarks = arguments['-ldisc']
label_type = 'disc'
else:
sct.printv('ERROR: Labels should be provided.', 1, 'error')
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = ''
param.path_qc = arguments.get("-qc", None)
path_template = arguments['-t']
contrast_template = arguments['-c']
ref = arguments['-ref']
param.remove_temp_files = int(arguments.get('-r'))
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
param.verbose = verbose # TODO: not clean, unify verbose or param.verbose in code, but not both
param_centerline = ParamCenterline(
algo_fitting=arguments['-centerline-algo'],
smooth=arguments['-centerline-smooth'])
# registration parameters
if '-param' in arguments:
# reset parameters but keep step=0 (might be overwritten if user specified step=0)
paramreg = ParamregMultiStep([step0])
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# add user parameters
for paramStep in arguments['-param']:
paramreg.addStep(paramStep)
else:
paramreg = ParamregMultiStep([step0, step1, step2])
# if ref=subject, initialize registration using different affine parameters
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# initialize other parameters
zsubsample = param.zsubsample
# retrieve template file names
file_template_vertebral_labeling = get_file_label(os.path.join(path_template, 'template'), 'vertebral labeling')
file_template = get_file_label(os.path.join(path_template, 'template'), contrast_template.upper() + '-weighted template')
file_template_seg = get_file_label(os.path.join(path_template, 'template'), 'spinal cord')
# start timer
start_time = time.time()
# get fname of the template + template objects
fname_template = os.path.join(path_template, 'template', file_template)
fname_template_vertebral_labeling = os.path.join(path_template, 'template', file_template_vertebral_labeling)
fname_template_seg = os.path.join(path_template, 'template', file_template_seg)
fname_template_disc_labeling = os.path.join(path_template, 'template', 'PAM50_label_disc.nii.gz')
# check file existence
# TODO: no need to do that!
sct.printv('\nCheck template files...')
sct.check_file_exist(fname_template, verbose)
sct.check_file_exist(fname_template_vertebral_labeling, verbose)
sct.check_file_exist(fname_template_seg, verbose)
path_data, file_data, ext_data = sct.extract_fname(fname_data)
# sct.printv(arguments)
sct.printv('\nCheck parameters:', verbose)
sct.printv(' Data: ' + fname_data, verbose)
sct.printv(' Landmarks: ' + fname_landmarks, verbose)
sct.printv(' Segmentation: ' + fname_seg, verbose)
sct.printv(' Path template: ' + path_template, verbose)
sct.printv(' Remove temp files: ' + str(param.remove_temp_files), verbose)
# check input labels
labels = check_labels(fname_landmarks, label_type=label_type)
vertebral_alignment = False
if len(labels) > 2 and label_type == 'disc':
vertebral_alignment = True
path_tmp = sct.tmp_create(basename="register_to_template", verbose=verbose)
# set temporary file names
ftmp_data = 'data.nii'
ftmp_seg = 'seg.nii.gz'
ftmp_label = 'label.nii.gz'
ftmp_template = 'template.nii'
ftmp_template_seg = 'template_seg.nii.gz'
ftmp_template_label = 'template_label.nii.gz'
# copy files to temporary folder
sct.printv('\nCopying input data to tmp folder and convert to nii...', verbose)
Image(fname_data).save(os.path.join(path_tmp, ftmp_data))
Image(fname_seg).save(os.path.join(path_tmp, ftmp_seg))
Image(fname_landmarks).save(os.path.join(path_tmp, ftmp_label))
Image(fname_template).save(os.path.join(path_tmp, ftmp_template))
Image(fname_template_seg).save(os.path.join(path_tmp, ftmp_template_seg))
Image(fname_template_vertebral_labeling).save(os.path.join(path_tmp, ftmp_template_label))
if label_type == 'disc':
Image(fname_template_disc_labeling).save(os.path.join(path_tmp, ftmp_template_label))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Generate labels from template vertebral labeling
if label_type == 'body':
sct.printv('\nGenerate labels from template vertebral labeling', verbose)
ftmp_template_label_, ftmp_template_label = ftmp_template_label, sct.add_suffix(ftmp_template_label, "_body")
sct_label_utils.main(args=['-i', ftmp_template_label_, '-vert-body', '0', '-o', ftmp_template_label])
# check if provided labels are available in the template
sct.printv('\nCheck if provided labels are available in the template', verbose)
image_label_template = Image(ftmp_template_label)
labels_template = image_label_template.getNonZeroCoordinates(sorting='value')
if labels[-1].value > labels_template[-1].value:
sct.printv('ERROR: Wrong landmarks input. Labels must have correspondence in template space. \nLabel max '
'provided: ' + str(labels[-1].value) + '\nLabel max from template: ' +
str(labels_template[-1].value), verbose, 'error')
# if only one label is present, force affine transformation to be Tx,Ty,Tz only (no scaling)
if len(labels) == 1:
paramreg.steps['0'].dof = 'Tx_Ty_Tz'
sct.printv('WARNING: Only one label is present. Forcing initial transformation to: ' + paramreg.steps['0'].dof,
1, 'warning')
# Project labels onto the spinal cord centerline because later, an affine transformation is estimated between the
# template's labels (centered in the cord) and the subject's labels (assumed to be centered in the cord).
# If labels are not centered, mis-registration errors are observed (see issue #1826)
ftmp_label = project_labels_on_spinalcord(ftmp_label, ftmp_seg, param_centerline)
# binarize segmentation (in case it has values below 0 caused by manual editing)
sct.printv('\nBinarize segmentation', verbose)
ftmp_seg_, ftmp_seg = ftmp_seg, sct.add_suffix(ftmp_seg, "_bin")
sct_maths.main(['-i', ftmp_seg_,
'-bin', '0.5',
'-o', ftmp_seg])
# Switch between modes: subject->template or template->subject
if ref == 'template':
# resample data to 1mm isotropic
sct.printv('\nResample data to 1mm isotropic...', verbose)
resample_file(ftmp_data, add_suffix(ftmp_data, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_data = add_suffix(ftmp_data, '_1mm')
resample_file(ftmp_seg, add_suffix(ftmp_seg, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_seg = add_suffix(ftmp_seg, '_1mm')
# N.B. resampling of labels is more complicated, because they are single-point labels, therefore resampling
# with nearest neighbour can make them disappear.
resample_labels(ftmp_label, ftmp_data, add_suffix(ftmp_label, '_1mm'))
ftmp_label = add_suffix(ftmp_label, '_1mm')
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
if vertebral_alignment:
# cropping the segmentation based on the label coverage to ensure good registration with vertebral alignment
# See https://github.com/neuropoly/spinalcordtoolbox/pull/1669 for details
image_labels = Image(ftmp_label)
coordinates_labels = image_labels.getNonZeroCoordinates(sorting='z')
nx, ny, nz, nt, px, py, pz, pt = image_labels.dim
offset_crop = 10.0 * pz # cropping the image 10 mm above and below the highest and lowest label
cropping_slices = [coordinates_labels[0].z - offset_crop, coordinates_labels[-1].z + offset_crop]
# make sure that the cropping slices do not extend outside of the slice range (issue #1811)
if cropping_slices[0] < 0:
cropping_slices[0] = 0
if cropping_slices[1] > nz:
cropping_slices[1] = nz
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, np.int32(np.round(cropping_slices))),))).save(ftmp_seg)
else:
# if we do not align the vertebral levels, we crop the segmentation from top to bottom
im_seg_rpi = Image(ftmp_seg_)
bottom = 0
for data in msct_image.SlicerOneAxis(im_seg_rpi, "IS"):
if (data != 0).any():
break
bottom += 1
top = im_seg_rpi.data.shape[2]
for data in msct_image.SlicerOneAxis(im_seg_rpi, "SI"):
if (data != 0).any():
break
top -= 1
msct_image.spatial_crop(im_seg_rpi, dict(((2, (bottom, top)),))).save(ftmp_seg)
# straighten segmentation
sct.printv('\nStraighten the spinal cord using centerline/segmentation...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
fn_warp_curve2straight = os.path.join(curdir, "warp_curve2straight.nii.gz")
fn_warp_straight2curve = os.path.join(curdir, "warp_straight2curve.nii.gz")
fn_straight_ref = os.path.join(curdir, "straight_ref.nii.gz")
cache_input_files=[ftmp_seg]
if vertebral_alignment:
cache_input_files += [
ftmp_template_seg,
ftmp_label,
ftmp_template_label,
]
cache_sig = sct.cache_signature(
input_files=cache_input_files,
)
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(fn_warp_curve2straight) and os.path.isfile(fn_warp_straight2curve) and os.path.isfile(fn_straight_ref):
sct.printv('Reusing existing warping field which seems to be valid', verbose, 'warning')
sct.copy(fn_warp_curve2straight, 'warp_curve2straight.nii.gz')
sct.copy(fn_warp_straight2curve, 'warp_straight2curve.nii.gz')
sct.copy(fn_straight_ref, 'straight_ref.nii.gz')
# apply straightening
sct_apply_transfo.main(args=[
'-i', ftmp_seg,
'-w', 'warp_curve2straight.nii.gz',
'-d', 'straight_ref.nii.gz',
'-o', add_suffix(ftmp_seg, '_straight')])
else:
from spinalcordtoolbox.straightening import SpinalCordStraightener
sc_straight = SpinalCordStraightener(ftmp_seg, ftmp_seg)
sc_straight.param_centerline = param_centerline
sc_straight.output_filename = add_suffix(ftmp_seg, '_straight')
sc_straight.path_output = './'
sc_straight.qc = '0'
sc_straight.remove_temp_files = param.remove_temp_files
sc_straight.verbose = verbose
if vertebral_alignment:
sc_straight.centerline_reference_filename = ftmp_template_seg
sc_straight.use_straight_reference = True
sc_straight.discs_input_filename = ftmp_label
sc_straight.discs_ref_filename = ftmp_template_label
sc_straight.straighten()
sct.cache_save(cachefile, cache_sig)
# N.B. DO NOT UPDATE VARIABLE ftmp_seg BECAUSE TEMPORARY USED LATER
# re-define warping field using non-cropped space (to avoid issue #367)
sct_concat_transfo.main(args=[
'-w', 'warp_straight2curve.nii.gz',
'-d', ftmp_data,
'-o', 'warp_straight2curve.nii.gz'])
if vertebral_alignment:
sct.copy('warp_curve2straight.nii.gz', 'warp_curve2straightAffine.nii.gz')
else:
# Label preparation:
# --------------------------------------------------------------------------------
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Dilating the input label so they can be straighten without losing them
sct.printv('\nDilating input labels using 3vox ball radius')
sct_maths.main(['-i', ftmp_label,
'-dilate', '3',
'-o', add_suffix(ftmp_label, '_dilate')])
ftmp_label = add_suffix(ftmp_label, '_dilate')
# Apply straightening to labels
sct.printv('\nApply straightening to labels...', verbose)
sct_apply_transfo.main(args=[
'-i', ftmp_label,
'-o', add_suffix(ftmp_label, '_straight'),
'-d', add_suffix(ftmp_seg, '_straight'),
'-w', 'warp_curve2straight.nii.gz',
'-x', 'nn'])
ftmp_label = add_suffix(ftmp_label, '_straight')
# Compute rigid transformation straight landmarks --> template landmarks
sct.printv('\nEstimate transformation for step #0...', verbose)
try:
register_landmarks(ftmp_label, ftmp_template_label, paramreg.steps['0'].dof,
fname_affine='straight2templateAffine.txt', verbose=verbose)
except RuntimeError:
raise('Input labels do not seem to be at the right place. Please check the position of the labels. '
'See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42')
# Concatenate transformations: curve --> straight --> affine
sct.printv('\nConcatenate transformations: curve --> straight --> affine...', verbose)
sct_concat_transfo.main(args=[
'-w', ['warp_curve2straight.nii.gz', 'straight2templateAffine.txt'],
'-d', 'template.nii',
'-o', 'warp_curve2straightAffine.nii.gz'])
# Apply transformation
sct.printv('\nApply transformation...', verbose)
sct_apply_transfo.main(args=[
'-i', ftmp_data,
'-o', add_suffix(ftmp_data, '_straightAffine'),
'-d', ftmp_template,
'-w', 'warp_curve2straightAffine.nii.gz'])
ftmp_data = add_suffix(ftmp_data, '_straightAffine')
sct_apply_transfo.main(args=[
'-i', ftmp_seg,
'-o', add_suffix(ftmp_seg, '_straightAffine'),
'-d', ftmp_template,
'-w', 'warp_curve2straightAffine.nii.gz',
'-x', 'linear'])
ftmp_seg = add_suffix(ftmp_seg, '_straightAffine')
"""
# Benjamin: Issue from Allan Martin, about the z=0 slice that is screwed up, caused by the affine transform.
# Solution found: remove slices below and above landmarks to avoid rotation effects
points_straight = []
for coord in landmark_template:
points_straight.append(coord.z)
min_point, max_point = int(np.round(np.min(points_straight))), int(np.round(np.max(points_straight)))
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_black')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (min_point,max_point)),))).save(ftmp_seg)
"""
# open segmentation
im = Image(ftmp_seg)
im_new = msct_image.empty_like(im)
# binarize
im_new.data = im.data > 0.5
# find min-max of anat2template (for subsequent cropping)
zmin_template, zmax_template = msct_image.find_zmin_zmax(im_new, threshold=0.5)
# save binarized segmentation
im_new.save(add_suffix(ftmp_seg, '_bin')) # unused?
# crop template in z-direction (for faster processing)
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nCrop data in template space (for faster processing)...', verbose)
ftmp_template_, ftmp_template = ftmp_template, add_suffix(ftmp_template, '_crop')
msct_image.spatial_crop(Image(ftmp_template_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template)
ftmp_template_seg_, ftmp_template_seg = ftmp_template_seg, add_suffix(ftmp_template_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_template_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template_seg)
ftmp_data_, ftmp_data = ftmp_data, add_suffix(ftmp_data, '_crop')
msct_image.spatial_crop(Image(ftmp_data_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_data)
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_seg)
# sub-sample in z-direction
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nSub-sample in z-direction (for faster processing)...', verbose)
sct.run(['sct_resample', '-i', ftmp_template, '-o', add_suffix(ftmp_template, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template = add_suffix(ftmp_template, '_sub')
sct.run(['sct_resample', '-i', ftmp_template_seg, '-o', add_suffix(ftmp_template_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template_seg = add_suffix(ftmp_template_seg, '_sub')
sct.run(['sct_resample', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_data = add_suffix(ftmp_data, '_sub')
sct.run(['sct_resample', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_seg = add_suffix(ftmp_seg, '_sub')
# Registration straight spinal cord to template
sct.printv('\nRegister straight spinal cord to template...', verbose)
# loop across registration steps
warp_forward = []
warp_inverse = []
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_data
dest = ftmp_template
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_seg
dest = ftmp_template_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog':
src_seg = ftmp_seg
dest_seg = ftmp_template_seg
# if step>1, apply warp_forward_concat to the src image to be used
if i_step > 1:
# apply transformation from previous step, to use as new src for registration
sct_apply_transfo.main(args=[
'-i', src,
'-d', dest,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src = add_suffix(src, '_regStep' + str(i_step - 1))
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # also apply transformation to the seg
sct_apply_transfo.main(args=[
'-i', src_seg,
'-d', dest_seg,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src_seg = add_suffix(src_seg, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # im_seg case
warp_forward_out, warp_inverse_out = register([src, src_seg], [dest, dest_seg], paramreg, param, str(i_step))
else:
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.append(warp_inverse_out)
# Concatenate transformations: anat --> template
sct.printv('\nConcatenate transformations: anat --> template...', verbose)
warp_forward.insert(0, 'warp_curve2straightAffine.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_forward,
'-d', 'template.nii',
'-o', 'warp_anat2template.nii.gz'])
# Concatenate transformations: template --> anat
sct.printv('\nConcatenate transformations: template --> anat...', verbose)
warp_inverse.reverse()
if vertebral_alignment:
warp_inverse.append('warp_straight2curve.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
else:
warp_inverse.append('straight2templateAffine.txt')
warp_inverse.append('warp_straight2curve.nii.gz')
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-winv', ['straight2templateAffine.txt'],
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
# register template->subject
elif ref == 'subject':
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Add one label because at least 3 orthogonal labels are required to estimate an affine transformation. This
# new label is added at the level of the upper most label (lowest value), at 1cm to the right.
for i_file in [ftmp_label, ftmp_template_label]:
im_label = Image(i_file)
coord_label = im_label.getCoordinatesAveragedByValue() # N.B. landmarks are sorted by value
# Create new label
from copy import deepcopy
new_label = deepcopy(coord_label[0])
# move it 5mm to the left (orientation is RAS)
nx, ny, nz, nt, px, py, pz, pt = im_label.dim
new_label.x = np.round(coord_label[0].x + 5.0 / px)
# assign value 99
new_label.value = 99
# Add to existing image
im_label.data[int(new_label.x), int(new_label.y), int(new_label.z)] = new_label.value
# Overwrite label file
# im_label.absolutepath = 'label_rpi_modif.nii.gz'
im_label.save()
# Bring template to subject space using landmark-based transformation
sct.printv('\nEstimate transformation for step #0...', verbose)
warp_forward = ['template2subjectAffine.txt']
warp_inverse = ['template2subjectAffine.txt']
try:
register_landmarks(ftmp_template_label, ftmp_label, paramreg.steps['0'].dof, fname_affine=warp_forward[0], verbose=verbose, path_qc="./")
except Exception:
sct.printv('ERROR: input labels do not seem to be at the right place. Please check the position of the labels. See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42', verbose=verbose, type='error')
# loop across registration steps
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_template
dest = ftmp_data
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_template_seg
dest = ftmp_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
# apply transformation from previous step, to use as new src for registration
sct_apply_transfo.main(args=[
'-i', src,
'-d', dest,
'-w', warp_forward,
'-o', add_suffix(src, '_regStep' + str(i_step - 1)),
'-x', interp_step])
src = add_suffix(src, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: template --> subject...', verbose)
sct_concat_transfo.main(args=[
'-w', warp_forward,
'-d', 'data.nii',
'-o', 'warp_template2anat.nii.gz'])
sct.printv('\nConcatenate transformations: subject --> template...', verbose)
sct_concat_transfo.main(args=[
'-w', warp_inverse,
'-winv', ['template2subjectAffine.txt'],
'-d', 'template.nii',
'-o', 'warp_anat2template.nii.gz'])
# Apply warping fields to anat and template
sct.run(['sct_apply_transfo', '-i', 'template.nii', '-o', 'template2anat.nii.gz', '-d', 'data.nii', '-w', 'warp_template2anat.nii.gz', '-crop', '1'], verbose)
sct.run(['sct_apply_transfo', '-i', 'data.nii', '-o', 'anat2template.nii.gz', '-d', 'template.nii', '-w', 'warp_anat2template.nii.gz', '-crop', '1'], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
fname_template2anat = os.path.join(path_output, 'template2anat' + ext_data)
fname_anat2template = os.path.join(path_output, 'anat2template' + ext_data)
sct.generate_output_file(os.path.join(path_tmp, "warp_template2anat.nii.gz"), os.path.join(path_output, "warp_template2anat.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_anat2template.nii.gz"), os.path.join(path_output, "warp_anat2template.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "template2anat.nii.gz"), fname_template2anat, verbose)
sct.generate_output_file(os.path.join(path_tmp, "anat2template.nii.gz"), fname_anat2template, verbose)
if ref == 'template':
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose)
# Delete temporary files
if param.remove_temp_files:
sct.printv('\nDelete temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if param.path_qc is not None:
generate_qc(fname_data, fname_in2=fname_template2anat, fname_seg=fname_seg, args=args,
path_qc=os.path.abspath(param.path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_to_template')
sct.display_viewer_syntax([fname_data, fname_template2anat], verbose=verbose)
sct.display_viewer_syntax([fname_template, fname_anat2template], verbose=verbose)
def deep_segmentation_spinalcord(im_image, contrast_type, ctr_algo='cnn', ctr_file=None, brain_bool=True,
kernel_size='2d', remove_temp_files=1, verbose=1):
"""Pipeline"""
# create temporary folder with intermediate results
tmp_folder = sct.TempFolder(verbose=verbose)
tmp_folder_path = tmp_folder.get_path()
if ctr_algo == 'file': # if the ctr_file is provided
tmp_folder.copy_from(ctr_file)
file_ctr = os.path.basename(ctr_file)
else:
file_ctr = None
tmp_folder.chdir()
# re-orient image to RPI
logger.info("Reorient the image to RPI, if necessary...")
original_orientation = im_image.orientation
fname_orient = 'image_in_RPI.nii'
im_image.change_orientation('RPI').save(fname_orient)
input_resolution = im_image.dim[4:7]
# find the spinal cord centerline - execute OptiC binary
logger.info("Finding the spinal cord centerline...")
fname_res, centerline_filename = find_centerline(algo=ctr_algo,
image_fname=fname_orient,
contrast_type=contrast_type,
brain_bool=brain_bool,
folder_output=tmp_folder_path,
remove_temp_files=remove_temp_files,
centerline_fname=file_ctr)
im_nii, ctr_nii = Image(fname_res), Image(centerline_filename)
# crop image around the spinal cord centerline
logger.info("Cropping the image around the spinal cord...")
crop_size = 96 if (kernel_size == '3d' and contrast_type == 't2s') else 64
X_CROP_LST, Y_CROP_LST, Z_CROP_LST, im_crop_nii = crop_image_around_centerline(im_in=im_nii,
ctr_in=ctr_nii,
crop_size=crop_size)
del ctr_nii
# normalize the intensity of the images
logger.info("Normalizing the intensity...")
im_norm_in = apply_intensity_normalization(im_in=im_crop_nii)
del im_crop_nii
if kernel_size == '2d':
# segment data using 2D convolutions
logger.info("Segmenting the spinal cord using deep learning on 2D patches...")
segmentation_model_fname = \
os.path.join(sct.__sct_dir__, 'data', 'deepseg_sc_models', '{}_sc.h5'.format(contrast_type))
seg_crop_data = segment_2d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
input_size=(crop_size, crop_size),
im_in=im_norm_in)
elif kernel_size == '3d':
# segment data using 3D convolutions
logger.info("Segmenting the spinal cord using deep learning on 3D patches...")
segmentation_model_fname = \
os.path.join(sct.__sct_dir__, 'data', 'deepseg_sc_models', '{}_sc_3D.h5'.format(contrast_type))
seg_crop_data = segment_3d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
im_in=im_norm_in)
del im_norm_in
# reconstruct the segmentation from the crop data
logger.info("Reassembling the image...")
seg_uncrop_nii = uncrop_image(ref_in=im_nii,
data_crop=seg_crop_data,
x_crop_lst=X_CROP_LST,
y_crop_lst=Y_CROP_LST,
z_crop_lst=Z_CROP_LST)
fname_res_seg = sct.add_suffix(fname_res, '_seg')
seg_uncrop_nii.save(fname_res_seg)
del seg_crop_data
# resample to initial resolution
logger.info("Resampling the segmentation to the original image resolution...")
initial_resolution = 'x'.join([str(input_resolution[0]), str(input_resolution[1]), str(input_resolution[2])])
fname_res_seg_downsamp = sct.add_suffix(fname_res_seg, '_downsamp')
resampling.resample_file(fname_res_seg, fname_res_seg_downsamp, initial_resolution,
'mm', 'linear', verbose=0)
im_image_res_seg_downsamp = Image(fname_res_seg_downsamp)
if ctr_algo == 'viewer': # resample and reorient the viewer labels
fname_res_labels = sct.add_suffix(fname_orient, '_labels-centerline')
resampling.resample_file(fname_res_labels, fname_res_labels, initial_resolution, 'mm', 'linear', verbose=0)
im_image_res_labels_downsamp = Image(fname_res_labels).change_orientation(original_orientation)
else:
im_image_res_labels_downsamp = None
if verbose == 2:
fname_res_ctr = sct.add_suffix(fname_orient, '_ctr')
resampling.resample_file(fname_res_ctr, fname_res_ctr, initial_resolution, 'mm', 'linear', verbose=0)
im_image_res_ctr_downsamp = Image(fname_res_ctr).change_orientation(original_orientation)
else:
im_image_res_ctr_downsamp = None
# binarize the resampled image to remove interpolation effects
logger.info("Binarizing the segmentation to avoid interpolation effects...")
thr = 0.0001 if contrast_type in ['t1', 'dwi'] else 0.5
# TODO: optimize speed --> np.where is slow
im_image_res_seg_downsamp.data[np.where(im_image_res_seg_downsamp.data >= thr)] = 1
im_image_res_seg_downsamp.data[np.where(im_image_res_seg_downsamp.data < thr)] = 0
# post processing step to z_regularized
im_image_res_seg_downsamp_postproc = post_processing_volume_wise(im_in=im_image_res_seg_downsamp)
tmp_folder.chdir_undo()
# remove temporary files
if remove_temp_files:
logger.info("Remove temporary files...")
tmp_folder.cleanup()
# reorient to initial orientation
return im_image_res_seg_downsamp_postproc.change_orientation(original_orientation), \
im_nii, \
seg_uncrop_nii.change_orientation('RPI'), \
im_image_res_labels_downsamp, \
im_image_res_ctr_downsamp
def find_centerline(algo, image_fname, contrast_type, brain_bool, folder_output, remove_temp_files, centerline_fname):
"""
Assumes RPI orientation
:param algo:
:param image_fname:
:param contrast_type:
:param brain_bool:
:param folder_output:
:param remove_temp_files:
:param centerline_fname:
:return:
"""
# TODO: remove unnecessary i/o
if Image(image_fname).dim[2] == 1: # isct_spine_detect requires nz > 1
im_concat = concat_data([image_fname, image_fname], dim=2)
im_concat.save(sct.add_suffix(image_fname, '_concat'))
image_fname = sct.add_suffix(image_fname, '_concat')
bool_2d = True
else:
bool_2d = False
# TODO: maybe change 'svm' for 'optic', because this is how we call it in sct_get_centerline
if algo == 'svm':
# run optic on a heatmap computed by a trained SVM+HoG algorithm
# optic_models_fname = os.path.join(path_sct, 'data', 'optic_models', '{}_model'.format(contrast_type))
# # TODO: replace with get_centerline(method=optic)
img_ctl, arr_ctl, _ = get_centerline(Image(image_fname), algo_fitting='optic', contrast=contrast_type)
centerline_filename = sct.add_suffix(image_fname, "_ctr")
img_ctl.save(centerline_filename)
elif algo == 'cnn':
# CNN parameters
dct_patch_ctr = {'t2': {'size': (80, 80), 'mean': 51.1417, 'std': 57.4408},
't2s': {'size': (80, 80), 'mean': 68.8591, 'std': 71.4659},
't1': {'size': (80, 80), 'mean': 55.7359, 'std': 64.3149},
'dwi': {'size': (80, 80), 'mean': 55.744, 'std': 45.003}}
dct_params_ctr = {'t2': {'features': 16, 'dilation_layers': 2},
't2s': {'features': 8, 'dilation_layers': 3},
't1': {'features': 24, 'dilation_layers': 3},
'dwi': {'features': 8, 'dilation_layers': 2}}
# load model
ctr_model_fname = os.path.join(sct.__sct_dir__, 'data', 'deepseg_sc_models', '{}_ctr.h5'.format(contrast_type))
ctr_model = nn_architecture_ctr(height=dct_patch_ctr[contrast_type]['size'][0],
width=dct_patch_ctr[contrast_type]['size'][1],
channels=1,
classes=1,
features=dct_params_ctr[contrast_type]['features'],
depth=2,
temperature=1.0,
padding='same',
batchnorm=True,
dropout=0.0,
dilation_layers=dct_params_ctr[contrast_type]['dilation_layers'])
ctr_model.load_weights(ctr_model_fname)
logger.info("Resample the image to 0.5x0.5 mm in-plane resolution...")
fname_res = sct.add_suffix(image_fname, '_resampled')
input_resolution = Image(image_fname).dim[4:7]
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(image_fname, fname_res, new_resolution, 'mm', 'linear', verbose=0)
# compute the heatmap
fname_heatmap = sct.add_suffix(image_fname, "_heatmap")
img_filename = ''.join(sct.extract_fname(fname_heatmap)[:2])
fname_heatmap_nii = img_filename + '.nii'
z_max = heatmap(filename_in=fname_res,
filename_out=fname_heatmap_nii,
model=ctr_model,
patch_shape=dct_patch_ctr[contrast_type]['size'],
mean_train=dct_patch_ctr[contrast_type]['mean'],
std_train=dct_patch_ctr[contrast_type]['std'],
brain_bool=brain_bool)
# run optic on the heatmap
centerline_filename = sct.add_suffix(fname_heatmap, "_ctr")
heatmap2optic(fname_heatmap=fname_heatmap_nii,
lambda_value=7 if contrast_type == 't2s' else 1,
fname_out=centerline_filename,
z_max=z_max if brain_bool else None)
elif algo == 'viewer':
im_labels = _call_viewer_centerline(Image(image_fname))
im_centerline, arr_centerline, _ = get_centerline(im_labels)
centerline_filename = sct.add_suffix(image_fname, "_ctr")
labels_filename = sct.add_suffix(image_fname, "_labels-centerline")
im_centerline.save(centerline_filename)
im_labels.save(labels_filename)
elif algo == 'file':
centerline_filename = sct.add_suffix(image_fname, "_ctr")
# Re-orient the manual centerline
Image(centerline_fname).change_orientation('RPI').save(centerline_filename)
else:
logger.error('The parameter "-centerline" is incorrect. Please try again.')
sys.exit(1)
if algo != 'cnn':
logger.info("Resample the image to 0.5x0.5 mm in-plane resolution...")
fname_res = sct.add_suffix(image_fname, '_resampled')
input_resolution = Image(image_fname).dim[4:7]
new_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(image_fname, fname_res, new_resolution, 'mm', 'linear', verbose=0)
resampling.resample_file(centerline_filename, centerline_filename, new_resolution, 'mm', 'linear', verbose=0)
if bool_2d:
im_split_lst = split_data(Image(centerline_filename), dim=2)
im_split_lst[0].save(centerline_filename)
return fname_res, centerline_filename
def deep_segmentation_MSlesion(im_image, contrast_type, ctr_algo='svm', ctr_file=None, brain_bool=True,
remove_temp_files=1, verbose=1):
"""
Segment lesions from MRI data.
:param im_image: Image() object containing the lesions to segment
:param contrast_type: Constrast of the image. Need to use one supported by the CNN models.
:param ctr_algo: Algo to find the centerline. See sct_get_centerline
:param ctr_file: Centerline or segmentation (optional)
:param brain_bool: If brain if present or not in the image.
:param remove_temp_files:
:return:
"""
# create temporary folder with intermediate results
tmp_folder = sct.TempFolder(verbose=verbose)
tmp_folder_path = tmp_folder.get_path()
if ctr_algo == 'file': # if the ctr_file is provided
tmp_folder.copy_from(ctr_file)
file_ctr = os.path.basename(ctr_file)
else:
file_ctr = None
tmp_folder.chdir()
# orientation of the image, should be RPI
logger.info("\nReorient the image to RPI, if necessary...")
fname_in = im_image.absolutepath
original_orientation = im_image.orientation
fname_orient = 'image_in_RPI.nii'
im_image.change_orientation('RPI').save(fname_orient)
input_resolution = im_image.dim[4:7]
# find the spinal cord centerline - execute OptiC binary
logger.info("\nFinding the spinal cord centerline...")
contrast_type_ctr = contrast_type.split('_')[0]
fname_res, centerline_filename = find_centerline(algo=ctr_algo,
image_fname=fname_orient,
contrast_type=contrast_type_ctr,
brain_bool=brain_bool,
folder_output=tmp_folder_path,
remove_temp_files=remove_temp_files,
centerline_fname=file_ctr)
im_nii, ctr_nii = Image(fname_res), Image(centerline_filename)
# crop image around the spinal cord centerline
logger.info("\nCropping the image around the spinal cord...")
crop_size = 48
X_CROP_LST, Y_CROP_LST, Z_CROP_LST, im_crop_nii = crop_image_around_centerline(im_in=im_nii,
ctr_in=ctr_nii,
crop_size=crop_size)
del ctr_nii
# normalize the intensity of the images
logger.info("Normalizing the intensity...")
im_norm_in = apply_intensity_normalization(img=im_crop_nii, contrast=contrast_type)
del im_crop_nii
# resample to 0.5mm isotropic
fname_norm = sct.add_suffix(fname_orient, '_norm')
im_norm_in.save(fname_norm)
fname_res3d = sct.add_suffix(fname_norm, '_resampled3d')
resampling.resample_file(fname_norm, fname_res3d, '0.5x0.5x0.5', 'mm', 'linear',
verbose=0)
# segment data using 3D convolutions
logger.info("\nSegmenting the MS lesions using deep learning on 3D patches...")
segmentation_model_fname = os.path.join(sct.__sct_dir__, 'data', 'deepseg_lesion_models',
'{}_lesion.h5'.format(contrast_type))
fname_seg_crop_res = sct.add_suffix(fname_res3d, '_lesionseg')
im_res3d = Image(fname_res3d)
seg_im = segment_3d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
im=im_res3d.copy())
seg_im.save(fname_seg_crop_res)
del im_res3d, seg_im
# resample to the initial pz resolution
fname_seg_res2d = sct.add_suffix(fname_seg_crop_res, '_resampled2d')
initial_2d_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(fname_seg_crop_res, fname_seg_res2d, initial_2d_resolution,
'mm', 'linear', verbose=0)
seg_crop = Image(fname_seg_res2d)
# reconstruct the segmentation from the crop data
logger.info("\nReassembling the image...")
seg_uncrop_nii = uncrop_image(ref_in=im_nii, data_crop=seg_crop.copy().data, x_crop_lst=X_CROP_LST,
y_crop_lst=Y_CROP_LST, z_crop_lst=Z_CROP_LST)
fname_seg_res_RPI = sct.add_suffix(fname_in, '_res_RPI_seg')
seg_uncrop_nii.save(fname_seg_res_RPI)
del seg_crop
# resample to initial resolution
logger.info("Resampling the segmentation to the original image resolution...")
initial_resolution = 'x'.join([str(input_resolution[0]), str(input_resolution[1]), str(input_resolution[2])])
fname_seg_RPI = sct.add_suffix(fname_in, '_RPI_seg')
resampling.resample_file(fname_seg_res_RPI, fname_seg_RPI, initial_resolution,
'mm', 'linear', verbose=0)
seg_initres_nii = Image(fname_seg_RPI)
if ctr_algo == 'viewer': # resample and reorient the viewer labels
fname_res_labels = sct.add_suffix(fname_orient, '_labels-centerline')
resampling.resample_file(fname_res_labels, fname_res_labels, initial_resolution,
'mm', 'linear', verbose=0)
im_image_res_labels_downsamp = Image(fname_res_labels).change_orientation(original_orientation)
else:
im_image_res_labels_downsamp = None
if verbose == 2:
fname_res_ctr = sct.add_suffix(fname_orient, '_ctr')
resampling.resample_file(fname_res_ctr, fname_res_ctr, initial_resolution,
'mm', 'linear', verbose=0)
im_image_res_ctr_downsamp = Image(fname_res_ctr).change_orientation(original_orientation)
else:
im_image_res_ctr_downsamp = None
# binarize the resampled image to remove interpolation effects
logger.info("\nBinarizing the segmentation to avoid interpolation effects...")
thr = 0.1
seg_initres_nii.data[np.where(seg_initres_nii.data >= thr)] = 1
seg_initres_nii.data[np.where(seg_initres_nii.data < thr)] = 0
# reorient to initial orientation
logger.info("\nReorienting the segmentation to the original image orientation...")
tmp_folder.chdir_undo()
# remove temporary files
if remove_temp_files:
logger.info("\nRemove temporary files...")
tmp_folder.cleanup()
# reorient to initial orientation
return seg_initres_nii.change_orientation(original_orientation), im_image_res_labels_downsamp, im_image_res_ctr_downsamp
def deep_segmentation_MSlesion(im_image,
contrast_type,
ctr_algo='svm',
ctr_file=None,
brain_bool=True,
remove_temp_files=1,
verbose=1):
"""
Segment lesions from MRI data.
:param im_image: Image() object containing the lesions to segment
:param contrast_type: Constrast of the image. Need to use one supported by the CNN models.
:param ctr_algo: Algo to find the centerline. See sct_get_centerline
:param ctr_file: Centerline or segmentation (optional)
:param brain_bool: If brain if present or not in the image.
:param remove_temp_files:
:return:
"""
# create temporary folder with intermediate results
tmp_folder = sct.TempFolder(verbose=verbose)
tmp_folder_path = tmp_folder.get_path()
if ctr_algo == 'file': # if the ctr_file is provided
tmp_folder.copy_from(ctr_file)
file_ctr = os.path.basename(ctr_file)
else:
file_ctr = None
tmp_folder.chdir()
fname_in = im_image.absolutepath
# re-orient image to RPI
logger.info("Reorient the image to RPI, if necessary...")
original_orientation = im_image.orientation
# fname_orient = 'image_in_RPI.nii'
im_image.change_orientation('RPI')
input_resolution = im_image.dim[4:7]
# Resample image to 0.5mm in plane
im_image_res = \
resampling.resample_nib(im_image, new_size=[0.5, 0.5, im_image.dim[6]], new_size_type='mm', interpolation='linear')
fname_orient = 'image_in_RPI_res.nii'
im_image_res.save(fname_orient)
# find the spinal cord centerline - execute OptiC binary
logger.info("\nFinding the spinal cord centerline...")
contrast_type_ctr = contrast_type.split('_')[0]
_, im_ctl, im_labels_viewer = find_centerline(
algo=ctr_algo,
image_fname=fname_orient,
contrast_type=contrast_type_ctr,
brain_bool=brain_bool,
folder_output=tmp_folder_path,
remove_temp_files=remove_temp_files,
centerline_fname=file_ctr)
if ctr_algo == 'file':
im_ctl = \
resampling.resample_nib(im_ctl, new_size=[0.5, 0.5, im_image.dim[6]], new_size_type='mm', interpolation='linear')
# crop image around the spinal cord centerline
logger.info("\nCropping the image around the spinal cord...")
crop_size = 48
X_CROP_LST, Y_CROP_LST, Z_CROP_LST, im_crop_nii = crop_image_around_centerline(
im_in=im_image_res, ctr_in=im_ctl, crop_size=crop_size)
del im_ctl
# normalize the intensity of the images
logger.info("Normalizing the intensity...")
im_norm_in = apply_intensity_normalization(img=im_crop_nii,
contrast=contrast_type)
del im_crop_nii
# resample to 0.5mm isotropic
fname_norm = sct.add_suffix(fname_orient, '_norm')
im_norm_in.save(fname_norm)
fname_res3d = sct.add_suffix(fname_norm, '_resampled3d')
resampling.resample_file(fname_norm,
fname_res3d,
'0.5x0.5x0.5',
'mm',
'linear',
verbose=0)
# segment data using 3D convolutions
logger.info(
"\nSegmenting the MS lesions using deep learning on 3D patches...")
segmentation_model_fname = sct_dir_local_path(
'data', 'deepseg_lesion_models', '{}_lesion.h5'.format(contrast_type))
fname_seg_crop_res = sct.add_suffix(fname_res3d, '_lesionseg')
im_res3d = Image(fname_res3d)
seg_im = segment_3d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
im=im_res3d.copy())
seg_im.save(fname_seg_crop_res)
del im_res3d, seg_im
# resample to the initial pz resolution
fname_seg_res2d = sct.add_suffix(fname_seg_crop_res, '_resampled2d')
initial_2d_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(fname_seg_crop_res,
fname_seg_res2d,
initial_2d_resolution,
'mm',
'linear',
verbose=0)
seg_crop = Image(fname_seg_res2d)
# reconstruct the segmentation from the crop data
logger.info("\nReassembling the image...")
seg_uncrop_nii = uncrop_image(ref_in=im_image_res,
data_crop=seg_crop.copy().data,
x_crop_lst=X_CROP_LST,
y_crop_lst=Y_CROP_LST,
z_crop_lst=Z_CROP_LST)
fname_seg_res_RPI = sct.add_suffix(fname_in, '_res_RPI_seg')
seg_uncrop_nii.save(fname_seg_res_RPI)
del seg_crop
# resample to initial resolution
logger.info(
"Resampling the segmentation to the original image resolution...")
initial_resolution = 'x'.join([
str(input_resolution[0]),
str(input_resolution[1]),
str(input_resolution[2])
])
fname_seg_RPI = sct.add_suffix(fname_in, '_RPI_seg')
resampling.resample_file(fname_seg_res_RPI,
fname_seg_RPI,
initial_resolution,
'mm',
'linear',
verbose=0)
seg_initres_nii = Image(fname_seg_RPI)
if ctr_algo == 'viewer': # resample and reorient the viewer labels
im_labels_viewer_nib = nib.nifti1.Nifti1Image(
im_labels_viewer.data, im_labels_viewer.hdr.get_best_affine())
im_viewer_r_nib = resampling.resample_nib(im_labels_viewer_nib,
new_size=input_resolution,
new_size_type='mm',
interpolation='linear')
im_viewer = Image(
im_viewer_r_nib.get_data(),
hdr=im_viewer_r_nib.header,
orientation='RPI',
dim=im_viewer_r_nib.header.get_data_shape()).change_orientation(
original_orientation)
else:
im_viewer = None
if verbose == 2:
fname_res_ctr = sct.add_suffix(fname_orient, '_ctr')
resampling.resample_file(fname_res_ctr,
fname_res_ctr,
initial_resolution,
'mm',
'linear',
verbose=0)
im_image_res_ctr_downsamp = Image(fname_res_ctr).change_orientation(
original_orientation)
else:
im_image_res_ctr_downsamp = None
# binarize the resampled image to remove interpolation effects
logger.info(
"\nBinarizing the segmentation to avoid interpolation effects...")
thr = 0.1
seg_initres_nii.data[np.where(seg_initres_nii.data >= thr)] = 1
seg_initres_nii.data[np.where(seg_initres_nii.data < thr)] = 0
# change data type
seg_initres_nii.change_type(np.uint8)
# reorient to initial orientation
logger.info(
"\nReorienting the segmentation to the original image orientation...")
tmp_folder.chdir_undo()
# remove temporary files
if remove_temp_files:
logger.info("\nRemove temporary files...")
tmp_folder.cleanup()
# reorient to initial orientation
return seg_initres_nii.change_orientation(
original_orientation), im_viewer, im_image_res_ctr_downsamp
def deep_segmentation_MSlesion(im_image,
contrast_type,
ctr_algo='svm',
ctr_file=None,
brain_bool=True,
remove_temp_files=1):
"""
Segment lesions from MRI data.
:param im_image: Image() object containing the lesions to segment
:param contrast_type: Constrast of the image. Need to use one supported by the CNN models.
:param ctr_algo: Algo to find the centerline. See sct_get_centerline
:param ctr_file: Centerline or segmentation (optional)
:param brain_bool: If brain if present or not in the image.
:param remove_temp_files:
:return:
"""
# create temporary folder with intermediate results
sct.log.info("\nCreating temporary folder...")
tmp_folder = sct.TempFolder()
tmp_folder_path = tmp_folder.get_path()
if ctr_algo == 'manual': # if the ctr_file is provided
tmp_folder.copy_from(ctr_file)
file_ctr = os.path.basename(ctr_file)
else:
file_ctr = None
tmp_folder.chdir()
# orientation of the image, should be RPI
sct.log.info("\nReorient the image to RPI, if necessary...")
fname_in = im_image.absolutepath
original_orientation = im_image.orientation
fname_orient = 'image_in_RPI.nii'
im_image.change_orientation('RPI').save(fname_orient)
input_resolution = im_image.dim[4:7]
# find the spinal cord centerline - execute OptiC binary
sct.log.info("\nFinding the spinal cord centerline...")
contrast_type_ctr = contrast_type.split('_')[0]
fname_res, centerline_filename = find_centerline(
algo=ctr_algo,
image_fname=fname_orient,
contrast_type=contrast_type_ctr,
brain_bool=brain_bool,
folder_output=tmp_folder_path,
remove_temp_files=remove_temp_files,
centerline_fname=file_ctr)
im_nii, ctr_nii = Image(fname_res), Image(centerline_filename)
# crop image around the spinal cord centerline
sct.log.info("\nCropping the image around the spinal cord...")
crop_size = 48
X_CROP_LST, Y_CROP_LST, Z_CROP_LST, im_crop_nii = crop_image_around_centerline(
im_in=im_nii, ctr_in=ctr_nii, crop_size=crop_size)
del ctr_nii
# normalize the intensity of the images
sct.log.info("Normalizing the intensity...")
im_norm_in = apply_intensity_normalization(img=im_crop_nii,
contrast=contrast_type)
del im_crop_nii
# resample to 0.5mm isotropic
fname_norm = sct.add_suffix(fname_orient, '_norm')
im_norm_in.save(fname_norm)
fname_res3d = sct.add_suffix(fname_norm, '_resampled3d')
resampling.resample_file(fname_norm,
fname_res3d,
'0.5x0.5x0.5',
'mm',
'linear',
verbose=0)
# segment data using 3D convolutions
sct.log.info(
"\nSegmenting the MS lesions using deep learning on 3D patches...")
segmentation_model_fname = os.path.join(
sct.__sct_dir__, 'data', 'deepseg_lesion_models',
'{}_lesion.h5'.format(contrast_type))
fname_seg_crop_res = sct.add_suffix(fname_res3d, '_lesionseg')
im_res3d = Image(fname_res3d)
seg_im = segment_3d(model_fname=segmentation_model_fname,
contrast_type=contrast_type,
im=im_res3d.copy())
seg_im.save(fname_seg_crop_res)
del im_res3d, seg_im
# resample to the initial pz resolution
fname_seg_res2d = sct.add_suffix(fname_seg_crop_res, '_resampled2d')
initial_2d_resolution = 'x'.join(['0.5', '0.5', str(input_resolution[2])])
resampling.resample_file(fname_seg_crop_res,
fname_seg_res2d,
initial_2d_resolution,
'mm',
'linear',
verbose=0)
seg_crop = Image(fname_seg_res2d)
# reconstruct the segmentation from the crop data
sct.log.info("\nReassembling the image...")
seg_uncrop_nii = uncrop_image(ref_in=im_nii,
data_crop=seg_crop.copy().data,
x_crop_lst=X_CROP_LST,
y_crop_lst=Y_CROP_LST,
z_crop_lst=Z_CROP_LST)
fname_seg_res_RPI = sct.add_suffix(fname_in, '_res_RPI_seg')
seg_uncrop_nii.save(fname_seg_res_RPI)
del seg_crop
# resample to initial resolution
sct.log.info(
"Resampling the segmentation to the original image resolution...")
initial_resolution = 'x'.join([
str(input_resolution[0]),
str(input_resolution[1]),
str(input_resolution[2])
])
fname_seg_RPI = sct.add_suffix(fname_in, '_RPI_seg')
resampling.resample_file(fname_seg_res_RPI,
fname_seg_RPI,
initial_resolution,
'mm',
'linear',
verbose=0)
seg_initres_nii = Image(fname_seg_RPI)
# binarize the resampled image to remove interpolation effects
sct.log.info(
"\nBinarizing the segmentation to avoid interpolation effects...")
thr = 0.1
seg_initres_nii.data[np.where(seg_initres_nii.data >= thr)] = 1
seg_initres_nii.data[np.where(seg_initres_nii.data < thr)] = 0
# reorient to initial orientation
sct.log.info(
"\nReorienting the segmentation to the original image orientation...")
tmp_folder.chdir_undo()
# remove temporary files
if remove_temp_files:
sct.log.info("\nRemove temporary files...")
tmp_folder.cleanup()
# reorient to initial orientation
return seg_initres_nii.change_orientation(original_orientation)
def main(args=None):
# initializations
param = Param()
# check user arguments
if not args:
args = sys.argv[1:]
# Get parser info
parser = get_parser()
arguments = parser.parse(args)
fname_data = arguments['-i']
fname_seg = arguments['-s']
if '-l' in arguments:
fname_landmarks = arguments['-l']
label_type = 'body'
elif '-ldisc' in arguments:
fname_landmarks = arguments['-ldisc']
label_type = 'disc'
else:
sct.printv('ERROR: Labels should be provided.', 1, 'error')
if '-ofolder' in arguments:
path_output = arguments['-ofolder']
else:
path_output = ''
param.path_qc = arguments.get("-qc", None)
path_template = arguments['-t']
contrast_template = arguments['-c']
ref = arguments['-ref']
param.remove_temp_files = int(arguments.get('-r'))
verbose = int(arguments.get('-v'))
sct.init_sct(log_level=verbose, update=True) # Update log level
param.verbose = verbose # TODO: not clean, unify verbose or param.verbose in code, but not both
param.straighten_fitting = arguments['-straighten-fitting']
# if '-cpu-nb' in arguments:
# arg_cpu = ' -cpu-nb '+str(arguments['-cpu-nb'])
# else:
# arg_cpu = ''
# registration parameters
if '-param' in arguments:
# reset parameters but keep step=0 (might be overwritten if user specified step=0)
paramreg = ParamregMultiStep([step0])
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# add user parameters
for paramStep in arguments['-param']:
paramreg.addStep(paramStep)
else:
paramreg = ParamregMultiStep([step0, step1, step2])
# if ref=subject, initialize registration using different affine parameters
if ref == 'subject':
paramreg.steps['0'].dof = 'Tx_Ty_Tz_Rx_Ry_Rz_Sz'
# initialize other parameters
zsubsample = param.zsubsample
# retrieve template file names
file_template_vertebral_labeling = get_file_label(os.path.join(path_template, 'template'), 'vertebral labeling')
file_template = get_file_label(os.path.join(path_template, 'template'), contrast_template.upper() + '-weighted template')
file_template_seg = get_file_label(os.path.join(path_template, 'template'), 'spinal cord')
# start timer
start_time = time.time()
# get fname of the template + template objects
fname_template = os.path.join(path_template, 'template', file_template)
fname_template_vertebral_labeling = os.path.join(path_template, 'template', file_template_vertebral_labeling)
fname_template_seg = os.path.join(path_template, 'template', file_template_seg)
fname_template_disc_labeling = os.path.join(path_template, 'template', 'PAM50_label_disc.nii.gz')
# check file existence
# TODO: no need to do that!
sct.printv('\nCheck template files...')
sct.check_file_exist(fname_template, verbose)
sct.check_file_exist(fname_template_vertebral_labeling, verbose)
sct.check_file_exist(fname_template_seg, verbose)
path_data, file_data, ext_data = sct.extract_fname(fname_data)
# sct.printv(arguments)
sct.printv('\nCheck parameters:', verbose)
sct.printv(' Data: ' + fname_data, verbose)
sct.printv(' Landmarks: ' + fname_landmarks, verbose)
sct.printv(' Segmentation: ' + fname_seg, verbose)
sct.printv(' Path template: ' + path_template, verbose)
sct.printv(' Remove temp files: ' + str(param.remove_temp_files), verbose)
# check input labels
labels = check_labels(fname_landmarks, label_type=label_type)
vertebral_alignment = False
if len(labels) > 2 and label_type == 'disc':
vertebral_alignment = True
path_tmp = sct.tmp_create(basename="register_to_template", verbose=verbose)
# set temporary file names
ftmp_data = 'data.nii'
ftmp_seg = 'seg.nii.gz'
ftmp_label = 'label.nii.gz'
ftmp_template = 'template.nii'
ftmp_template_seg = 'template_seg.nii.gz'
ftmp_template_label = 'template_label.nii.gz'
# copy files to temporary folder
sct.printv('\nCopying input data to tmp folder and convert to nii...', verbose)
Image(fname_data).save(os.path.join(path_tmp, ftmp_data))
Image(fname_seg).save(os.path.join(path_tmp, ftmp_seg))
Image(fname_landmarks).save(os.path.join(path_tmp, ftmp_label))
Image(fname_template).save(os.path.join(path_tmp, ftmp_template))
Image(fname_template_seg).save(os.path.join(path_tmp, ftmp_template_seg))
Image(fname_template_vertebral_labeling).save(os.path.join(path_tmp, ftmp_template_label))
if label_type == 'disc':
Image(fname_template_disc_labeling).save(os.path.join(path_tmp, ftmp_template_label))
# go to tmp folder
curdir = os.getcwd()
os.chdir(path_tmp)
# Generate labels from template vertebral labeling
if label_type == 'body':
sct.printv('\nGenerate labels from template vertebral labeling', verbose)
ftmp_template_label_, ftmp_template_label = ftmp_template_label, sct.add_suffix(ftmp_template_label, "_body")
sct_label_utils.main(args=['-i', ftmp_template_label_, '-vert-body', '0', '-o', ftmp_template_label])
# check if provided labels are available in the template
sct.printv('\nCheck if provided labels are available in the template', verbose)
image_label_template = Image(ftmp_template_label)
labels_template = image_label_template.getNonZeroCoordinates(sorting='value')
if labels[-1].value > labels_template[-1].value:
sct.printv('ERROR: Wrong landmarks input. Labels must have correspondence in template space. \nLabel max '
'provided: ' + str(labels[-1].value) + '\nLabel max from template: ' +
str(labels_template[-1].value), verbose, 'error')
# if only one label is present, force affine transformation to be Tx,Ty,Tz only (no scaling)
if len(labels) == 1:
paramreg.steps['0'].dof = 'Tx_Ty_Tz'
sct.printv('WARNING: Only one label is present. Forcing initial transformation to: ' + paramreg.steps['0'].dof,
1, 'warning')
# Project labels onto the spinal cord centerline because later, an affine transformation is estimated between the
# template's labels (centered in the cord) and the subject's labels (assumed to be centered in the cord).
# If labels are not centered, mis-registration errors are observed (see issue #1826)
ftmp_label = project_labels_on_spinalcord(ftmp_label, ftmp_seg)
# binarize segmentation (in case it has values below 0 caused by manual editing)
sct.printv('\nBinarize segmentation', verbose)
ftmp_seg_, ftmp_seg = ftmp_seg, sct.add_suffix(ftmp_seg, "_bin")
sct_maths.main(['-i', ftmp_seg_,
'-bin', '0.5',
'-o', ftmp_seg])
# Switch between modes: subject->template or template->subject
if ref == 'template':
# resample data to 1mm isotropic
sct.printv('\nResample data to 1mm isotropic...', verbose)
resample_file(ftmp_data, add_suffix(ftmp_data, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_data = add_suffix(ftmp_data, '_1mm')
resample_file(ftmp_seg, add_suffix(ftmp_seg, '_1mm'), '1.0x1.0x1.0', 'mm', 'linear', verbose)
ftmp_seg = add_suffix(ftmp_seg, '_1mm')
# N.B. resampling of labels is more complicated, because they are single-point labels, therefore resampling
# with nearest neighbour can make them disappear.
resample_labels(ftmp_label, ftmp_data, add_suffix(ftmp_label, '_1mm'))
ftmp_label = add_suffix(ftmp_label, '_1mm')
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
if vertebral_alignment:
# cropping the segmentation based on the label coverage to ensure good registration with vertebral alignment
# See https://github.com/neuropoly/spinalcordtoolbox/pull/1669 for details
image_labels = Image(ftmp_label)
coordinates_labels = image_labels.getNonZeroCoordinates(sorting='z')
nx, ny, nz, nt, px, py, pz, pt = image_labels.dim
offset_crop = 10.0 * pz # cropping the image 10 mm above and below the highest and lowest label
cropping_slices = [coordinates_labels[0].z - offset_crop, coordinates_labels[-1].z + offset_crop]
# make sure that the cropping slices do not extend outside of the slice range (issue #1811)
if cropping_slices[0] < 0:
cropping_slices[0] = 0
if cropping_slices[1] > nz:
cropping_slices[1] = nz
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, np.int32(np.round(cropping_slices))),))).save(ftmp_seg)
else:
# if we do not align the vertebral levels, we crop the segmentation from top to bottom
im_seg_rpi = Image(ftmp_seg_)
bottom = 0
for data in msct_image.SlicerOneAxis(im_seg_rpi, "IS"):
if (data != 0).any():
break
bottom += 1
top = im_seg_rpi.data.shape[2]
for data in msct_image.SlicerOneAxis(im_seg_rpi, "SI"):
if (data != 0).any():
break
top -= 1
msct_image.spatial_crop(im_seg_rpi, dict(((2, (bottom, top)),))).save(ftmp_seg)
# straighten segmentation
sct.printv('\nStraighten the spinal cord using centerline/segmentation...', verbose)
# check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time)
fn_warp_curve2straight = os.path.join(curdir, "warp_curve2straight.nii.gz")
fn_warp_straight2curve = os.path.join(curdir, "warp_straight2curve.nii.gz")
fn_straight_ref = os.path.join(curdir, "straight_ref.nii.gz")
cache_input_files=[ftmp_seg]
if vertebral_alignment:
cache_input_files += [
ftmp_template_seg,
ftmp_label,
ftmp_template_label,
]
cache_sig = sct.cache_signature(
input_files=cache_input_files,
)
cachefile = os.path.join(curdir, "straightening.cache")
if sct.cache_valid(cachefile, cache_sig) and os.path.isfile(fn_warp_curve2straight) and os.path.isfile(fn_warp_straight2curve) and os.path.isfile(fn_straight_ref):
sct.printv('Reusing existing warping field which seems to be valid', verbose, 'warning')
sct.copy(fn_warp_curve2straight, 'warp_curve2straight.nii.gz')
sct.copy(fn_warp_straight2curve, 'warp_straight2curve.nii.gz')
sct.copy(fn_straight_ref, 'straight_ref.nii.gz')
# apply straightening
sct.run(['sct_apply_transfo', '-i', ftmp_seg, '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', add_suffix(ftmp_seg, '_straight')])
else:
from spinalcordtoolbox.straightening import SpinalCordStraightener
sc_straight = SpinalCordStraightener(ftmp_seg, ftmp_seg)
sc_straight.algo_fitting = param.straighten_fitting
sc_straight.output_filename = add_suffix(ftmp_seg, '_straight')
sc_straight.path_output = './'
sc_straight.qc = '0'
sc_straight.remove_temp_files = param.remove_temp_files
sc_straight.verbose = verbose
if vertebral_alignment:
sc_straight.centerline_reference_filename = ftmp_template_seg
sc_straight.use_straight_reference = True
sc_straight.discs_input_filename = ftmp_label
sc_straight.discs_ref_filename = ftmp_template_label
sc_straight.straighten()
sct.cache_save(cachefile, cache_sig)
# N.B. DO NOT UPDATE VARIABLE ftmp_seg BECAUSE TEMPORARY USED LATER
# re-define warping field using non-cropped space (to avoid issue #367)
s, o = sct.run(['sct_concat_transfo', '-w', 'warp_straight2curve.nii.gz', '-d', ftmp_data, '-o', 'warp_straight2curve.nii.gz'])
if vertebral_alignment:
sct.copy('warp_curve2straight.nii.gz', 'warp_curve2straightAffine.nii.gz')
else:
# Label preparation:
# --------------------------------------------------------------------------------
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Dilating the input label so they can be straighten without losing them
sct.printv('\nDilating input labels using 3vox ball radius')
sct_maths.main(['-i', ftmp_label,
'-dilate', '3',
'-o', add_suffix(ftmp_label, '_dilate')])
ftmp_label = add_suffix(ftmp_label, '_dilate')
# Apply straightening to labels
sct.printv('\nApply straightening to labels...', verbose)
sct.run(['sct_apply_transfo', '-i', ftmp_label, '-o', add_suffix(ftmp_label, '_straight'), '-d', add_suffix(ftmp_seg, '_straight'), '-w', 'warp_curve2straight.nii.gz', '-x', 'nn'])
ftmp_label = add_suffix(ftmp_label, '_straight')
# Compute rigid transformation straight landmarks --> template landmarks
sct.printv('\nEstimate transformation for step #0...', verbose)
try:
register_landmarks(ftmp_label, ftmp_template_label, paramreg.steps['0'].dof,
fname_affine='straight2templateAffine.txt', verbose=verbose)
except RuntimeError:
raise('Input labels do not seem to be at the right place. Please check the position of the labels. '
'See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42')
# Concatenate transformations: curve --> straight --> affine
sct.printv('\nConcatenate transformations: curve --> straight --> affine...', verbose)
sct.run(['sct_concat_transfo', '-w', 'warp_curve2straight.nii.gz,straight2templateAffine.txt', '-d', 'template.nii', '-o', 'warp_curve2straightAffine.nii.gz'])
# Apply transformation
sct.printv('\nApply transformation...', verbose)
sct.run(['sct_apply_transfo', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_straightAffine'), '-d', ftmp_template, '-w', 'warp_curve2straightAffine.nii.gz'])
ftmp_data = add_suffix(ftmp_data, '_straightAffine')
sct.run(['sct_apply_transfo', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_straightAffine'), '-d', ftmp_template, '-w', 'warp_curve2straightAffine.nii.gz', '-x', 'linear'])
ftmp_seg = add_suffix(ftmp_seg, '_straightAffine')
"""
# Benjamin: Issue from Allan Martin, about the z=0 slice that is screwed up, caused by the affine transform.
# Solution found: remove slices below and above landmarks to avoid rotation effects
points_straight = []
for coord in landmark_template:
points_straight.append(coord.z)
min_point, max_point = int(np.round(np.min(points_straight))), int(np.round(np.max(points_straight)))
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_black')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (min_point,max_point)),))).save(ftmp_seg)
"""
# open segmentation
im = Image(ftmp_seg)
im_new = msct_image.empty_like(im)
# binarize
im_new.data = im.data > 0.5
# find min-max of anat2template (for subsequent cropping)
zmin_template, zmax_template = msct_image.find_zmin_zmax(im_new, threshold=0.5)
# save binarized segmentation
im_new.save(add_suffix(ftmp_seg, '_bin')) # unused?
# crop template in z-direction (for faster processing)
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nCrop data in template space (for faster processing)...', verbose)
ftmp_template_, ftmp_template = ftmp_template, add_suffix(ftmp_template, '_crop')
msct_image.spatial_crop(Image(ftmp_template_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template)
ftmp_template_seg_, ftmp_template_seg = ftmp_template_seg, add_suffix(ftmp_template_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_template_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_template_seg)
ftmp_data_, ftmp_data = ftmp_data, add_suffix(ftmp_data, '_crop')
msct_image.spatial_crop(Image(ftmp_data_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_data)
ftmp_seg_, ftmp_seg = ftmp_seg, add_suffix(ftmp_seg, '_crop')
msct_image.spatial_crop(Image(ftmp_seg_), dict(((2, (zmin_template,zmax_template)),))).save(ftmp_seg)
# sub-sample in z-direction
# TODO: refactor to use python module instead of doing i/o
sct.printv('\nSub-sample in z-direction (for faster processing)...', verbose)
sct.run(['sct_resample', '-i', ftmp_template, '-o', add_suffix(ftmp_template, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template = add_suffix(ftmp_template, '_sub')
sct.run(['sct_resample', '-i', ftmp_template_seg, '-o', add_suffix(ftmp_template_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_template_seg = add_suffix(ftmp_template_seg, '_sub')
sct.run(['sct_resample', '-i', ftmp_data, '-o', add_suffix(ftmp_data, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_data = add_suffix(ftmp_data, '_sub')
sct.run(['sct_resample', '-i', ftmp_seg, '-o', add_suffix(ftmp_seg, '_sub'), '-f', '1x1x' + zsubsample], verbose)
ftmp_seg = add_suffix(ftmp_seg, '_sub')
# Registration straight spinal cord to template
sct.printv('\nRegister straight spinal cord to template...', verbose)
# loop across registration steps
warp_forward = []
warp_inverse = []
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_data
dest = ftmp_template
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_seg
dest = ftmp_template_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog':
src_seg = ftmp_seg
dest_seg = ftmp_template_seg
# if step>1, apply warp_forward_concat to the src image to be used
if i_step > 1:
# apply transformation from previous step, to use as new src for registration
sct.run(['sct_apply_transfo', '-i', src, '-d', dest, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src = add_suffix(src, '_regStep' + str(i_step - 1))
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # also apply transformation to the seg
sct.run(['sct_apply_transfo', '-i', src_seg, '-d', dest_seg, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src_seg = add_suffix(src_seg, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
if paramreg.steps[str(i_step)].algo == 'centermassrot' and paramreg.steps[str(i_step)].rot_method == 'hog': # im_seg case
warp_forward_out, warp_inverse_out = register([src, src_seg], [dest, dest_seg], paramreg, param, str(i_step))
else:
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.append(warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: anat --> template...', verbose)
sct.run(['sct_concat_transfo', '-w', 'warp_curve2straightAffine.nii.gz,' + ','.join(warp_forward), '-d', 'template.nii', '-o', 'warp_anat2template.nii.gz'], verbose)
# sct.run('sct_concat_transfo -w warp_curve2straight.nii.gz,straight2templateAffine.txt,'+','.join(warp_forward)+' -d template.nii -o warp_anat2template.nii.gz', verbose)
sct.printv('\nConcatenate transformations: template --> anat...', verbose)
warp_inverse.reverse()
if vertebral_alignment:
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse) + ',warp_straight2curve.nii.gz', '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
else:
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse) + ',-straight2templateAffine.txt,warp_straight2curve.nii.gz', '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
# register template->subject
elif ref == 'subject':
# Change orientation of input images to RPI
sct.printv('\nChange orientation of input images to RPI...', verbose)
ftmp_data = Image(ftmp_data).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_seg = Image(ftmp_seg).change_orientation("RPI", generate_path=True).save().absolutepath
ftmp_label = Image(ftmp_label).change_orientation("RPI", generate_path=True).save().absolutepath
# Remove unused label on template. Keep only label present in the input label image
sct.printv('\nRemove unused label on template. Keep only label present in the input label image...', verbose)
sct.run(['sct_label_utils', '-i', ftmp_template_label, '-o', ftmp_template_label, '-remove-reference', ftmp_label])
# Add one label because at least 3 orthogonal labels are required to estimate an affine transformation. This
# new label is added at the level of the upper most label (lowest value), at 1cm to the right.
for i_file in [ftmp_label, ftmp_template_label]:
im_label = Image(i_file)
coord_label = im_label.getCoordinatesAveragedByValue() # N.B. landmarks are sorted by value
# Create new label
from copy import deepcopy
new_label = deepcopy(coord_label[0])
# move it 5mm to the left (orientation is RAS)
nx, ny, nz, nt, px, py, pz, pt = im_label.dim
new_label.x = np.round(coord_label[0].x + 5.0 / px)
# assign value 99
new_label.value = 99
# Add to existing image
im_label.data[int(new_label.x), int(new_label.y), int(new_label.z)] = new_label.value
# Overwrite label file
# im_label.absolutepath = 'label_rpi_modif.nii.gz'
im_label.save()
# Bring template to subject space using landmark-based transformation
sct.printv('\nEstimate transformation for step #0...', verbose)
warp_forward = ['template2subjectAffine.txt']
warp_inverse = ['-template2subjectAffine.txt']
try:
register_landmarks(ftmp_template_label, ftmp_label, paramreg.steps['0'].dof, fname_affine=warp_forward[0], verbose=verbose, path_qc="./")
except Exception:
sct.printv('ERROR: input labels do not seem to be at the right place. Please check the position of the labels. See documentation for more details: https://www.slideshare.net/neuropoly/sct-course-20190121/42', verbose=verbose, type='error')
# loop across registration steps
for i_step in range(1, len(paramreg.steps)):
sct.printv('\nEstimate transformation for step #' + str(i_step) + '...', verbose)
# identify which is the src and dest
if paramreg.steps[str(i_step)].type == 'im':
src = ftmp_template
dest = ftmp_data
interp_step = 'linear'
elif paramreg.steps[str(i_step)].type == 'seg':
src = ftmp_template_seg
dest = ftmp_seg
interp_step = 'nn'
else:
sct.printv('ERROR: Wrong image type.', 1, 'error')
# apply transformation from previous step, to use as new src for registration
sct.run(['sct_apply_transfo', '-i', src, '-d', dest, '-w', ','.join(warp_forward), '-o', add_suffix(src, '_regStep' + str(i_step - 1)), '-x', interp_step], verbose)
src = add_suffix(src, '_regStep' + str(i_step - 1))
# register src --> dest
# TODO: display param for debugging
warp_forward_out, warp_inverse_out = register(src, dest, paramreg, param, str(i_step))
warp_forward.append(warp_forward_out)
warp_inverse.insert(0, warp_inverse_out)
# Concatenate transformations:
sct.printv('\nConcatenate transformations: template --> subject...', verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_forward), '-d', 'data.nii', '-o', 'warp_template2anat.nii.gz'], verbose)
sct.printv('\nConcatenate transformations: subject --> template...', verbose)
sct.run(['sct_concat_transfo', '-w', ','.join(warp_inverse), '-d', 'template.nii', '-o', 'warp_anat2template.nii.gz'], verbose)
# Apply warping fields to anat and template
sct.run(['sct_apply_transfo', '-i', 'template.nii', '-o', 'template2anat.nii.gz', '-d', 'data.nii', '-w', 'warp_template2anat.nii.gz', '-crop', '1'], verbose)
sct.run(['sct_apply_transfo', '-i', 'data.nii', '-o', 'anat2template.nii.gz', '-d', 'template.nii', '-w', 'warp_anat2template.nii.gz', '-crop', '1'], verbose)
# come back
os.chdir(curdir)
# Generate output files
sct.printv('\nGenerate output files...', verbose)
fname_template2anat = os.path.join(path_output, 'template2anat' + ext_data)
fname_anat2template = os.path.join(path_output, 'anat2template' + ext_data)
sct.generate_output_file(os.path.join(path_tmp, "warp_template2anat.nii.gz"), os.path.join(path_output, "warp_template2anat.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_anat2template.nii.gz"), os.path.join(path_output, "warp_anat2template.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "template2anat.nii.gz"), fname_template2anat, verbose)
sct.generate_output_file(os.path.join(path_tmp, "anat2template.nii.gz"), fname_anat2template, verbose)
if ref == 'template':
# copy straightening files in case subsequent SCT functions need them
sct.generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose)
sct.generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose)
# Delete temporary files
if param.remove_temp_files:
sct.printv('\nDelete temporary files...', verbose)
sct.rmtree(path_tmp, verbose=verbose)
# display elapsed time
elapsed_time = time.time() - start_time
sct.printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's', verbose)
qc_dataset = arguments.get("-qc-dataset", None)
qc_subject = arguments.get("-qc-subject", None)
if param.path_qc is not None:
generate_qc(fname_data, fname_in2=fname_template2anat, fname_seg=fname_seg, args=args,
path_qc=os.path.abspath(param.path_qc), dataset=qc_dataset, subject=qc_subject,
process='sct_register_to_template')
sct.display_viewer_syntax([fname_data, fname_template2anat], verbose=verbose)
sct.display_viewer_syntax([fname_template, fname_anat2template], verbose=verbose)
