def jjm_count(start, delta, threshold=0, up=True, trace=None, mark=True): """ Counts the number of events (e.g action potentials (AP)) in the current trace. Arguments: start -- starting time (in ms) to look for events. delta -- time interval (in ms) to look for events. threshold -- (optional) detection threshold (default = 0). up -- (optional) True (default) will look for upward events, False downwards. trace -- (optional) zero-based index of the trace in the current channel, if None, the current trace is selected. mark -- (optional) if True (default), set a mark at the point of threshold crossing Returns: An integer with the number of events. Examples: count_events(500,1000) returns the number of events found between t=500 ms and t=1500 ms above 0 in the current trace and shows a stf marker. count_events(500,1000,0,False,-10,i) returns the number of events found below -10 in the trace i and shows the corresponding stf markers. """ # sets the current trace or the one given in trace. if trace is None: sweep = stf.get_trace_index() else: if type(trace) !=int: print "trace argument admits only integers" return False sweep = trace # set the trace described in sweep stf.set_trace(sweep) # transform time into sampling points dt = stf.get_sampling_interval() pstart = int( round(start/dt) ) pdelta = int( round(delta/dt) ) # select the section of interest within the trace selection = stf.get_trace()[pstart:(pstart+pdelta)] # algorithm to detect events EventCounter,i = 0,0 # set counter and index to zero # list of sample points sample_points = [] # choose comparator according to direction: if up: comp = lambda a, b: a > b else: comp = lambda a, b: a < b # run the loop while i<len(selection): if comp(selection[i],threshold): EventCounter +=1 if mark: sample_point = pstart+i; sample_points.append(sample_point); stf.set_marker(pstart+i, selection[i]) while i<len(selection) and comp(selection[i],threshold): i+=1 # skip values if index in bounds AND until the value is below/above threshold again else: i+=1 time_points = [sample_point*dt for sample_point in sample_points]; return (EventCounter, sample_points, time_points)
def find_ADPs(AP_peak_indicies): ADP_values = [] ADP_indicies = [] ##slices for peak in range(len(AP_peak_indicies)-1): ADP_search = stf.get_trace()[AP_peak_indicies[peak]:AP_peak_indicies[peak+1]] min_value = np.min(ADP_search) min_index = AP_peak_indicies[peak] + np.argmin(ADP_search) stf.set_marker(min_index, min_value) ADP_values.append(min_value) ADP_indicies.append(min_index) return(ADP_values, ADP_indicies)
def automated_search_triexponential(trace_region_to_search, search_period,
threshold, min_btw_events, tau_rise,
tau_1_decay, tau_2_decay):
"""searches section of trace based on a user input triexponential function (tau_rise, tau_1_decay, tau_2_decay)"""
#converts some inputs to sample points
min_samples_btw_events = min_btw_events / stf.get_sampling_interval()
#pull out region to search
region_to_search = stf.get_trace(
)[trace_region_to_search[0]:trace_region_to_search[1]]
#list to store detected events
event_times = []
#creates vector of time points
t = np.linspace(0, 50, (50 / stf.get_sampling_interval()))
#creates triexponential pattern function
p_t = [(1 - math.exp(-(t_point - 0) / tau_rise)) *
(math.exp(-(t_point - 0) / tau_1_decay)) *
(math.exp(-(t_point - 0) / tau_2_decay)) for t_point in t]
#slides window along
pt = 0
while pt < range(len(region_to_search) - int(min_samples_btw_events)):
EPSC_test = stf.get_trace()[pt:(
pt + (search_period / stf.get_sampling_interval()))]
corr_coeff = stats.pearsonr(p_t, EPSC_test)[0]
if corr_coeff > threshold:
stf.set_marker(pt,
region_to_search[trace_region_to_search[0] + pt])
event_times.append(pt * stf.get_sampling_interval())
pt += min_samples_btw_events
else:
pt += 1
return (event_times)
def find_thresholds(input_trace, input_trace_si, ADP_sample_points):
#take derivative of trace, assume relevant trace is set to current trace
#get_dv_dt_as_numpy(input_array, si, *argv)
_1stderivative = dv_dt_V.get_dv_dt_as_numpy(input_trace, input_trace_si)
#take 2nd derivative of trace
_2ndderivative = dv_dt_V.get_dv_dt_as_numpy(_1stderivative, input_trace_si)
_3rdderivative = dv_dt_V.get_dv_dt_as_numpy(_2ndderivative, input_trace_si)
#will need to filter trace here
#try with using sigma of 1
_3rdderivative_filtered = _3rdderivative
#trace_filtering.filter_1d_numpy(_3rdderivative, 1, False)
#find peak time points
#use ADP sample points, estimate an AP width of 10ms to work backword from to capture peak
threshold_points = []
threshold_indicies = []
for x in range(len(ADP_sample_points)):
if x == 0:
earlier_point = int(ADP_sample_points[x]) - int(
round(50 / input_trace_si))
else:
earlier_point = int(ADP_sample_points[x - 1])
point = ADP_sample_points[x]
deriv_peak_search = _3rdderivative_filtered[earlier_point:point]
threshold_points.append(np.max(deriv_peak_search))
threshold_index = earlier_point + np.argmax(deriv_peak_search)
threshold_indicies.append(threshold_index)
#marker function is not working here, not sure why, also maybe a good point to set a
#voltage bound on detecting the threshold
stf.set_marker(threshold_index, input_trace[threshold_index])
#use peak time points to pull out the voltage values from the 1st trace, these are voltage values
return (threshold_indicies)
def find_baseline_amplitude(sigma):
# gaussian filter with sigma 10
trace_ = stf.get_trace()
trace_filtered = ndimage.filters.gaussian_filter(trace_, sigma)
# take derivative
si = stf.get_sampling_interval()
#read V values from trace,
V_values = stf.get_trace()
#compute dv and by iterating over voltage vectors
dv = [V_values[i + 1] - V_values[i] for i in range(len(V_values) - 1)]
#compute dv/dt
dv_dt = [(dv[i] / si) for i in range(len(dv))]
# find index of derivative peak
deriv_max = np.argmin(dv_dt)
# use derivative peak index to get baseline from original trace
# use a mean of 10 sample points
baseline = np.mean(trace_[deriv_max - 10:deriv_max])
stf.set_marker(deriv_max, baseline)
peak_amplitude = np.min(stf.get_trace())
peak_index = np.argmin(stf.get_trace())
stf.set_marker(peak_index, peak_amplitude)
peak_from_baseline = peak_amplitude - baseline
return (baseline, peak_amplitude, peak_from_baseline)
def find_AP_peak_ADP_trace(*argv):
""" count number of APs, find ADPs and thesholds in indicated trace with current injection/gradually increasing steps
inputs: (time (msec) to start search, length of search region, starting current value,
current delta between traces, threshold value, deflection direction ('up'/'down'), mark traces (True/False))"""
##if times are input, use those, otherwise use peak cursor settings
#TO DO: optional change to threshold_values and deflection_direction
if len(argv) > 0:
trace_selection = argv[0]
threshold_value = float(argv[1])
deflection_direction = argv[2]
mark_option = argv[3]
start_msec = float(argv[4])
delta_msec = float(argv[5])
else:
trace_selection = stf.get_trace_index()
threshold_value = 0
deflection_direction = 'up'
mark_option = True
start_msec = float(stf.get_peak_start(True))
delta_msec = float(stf.get_peak_end(True) - start_msec)
stf.set_trace(trace_selection)
##gets AP counts and sample points in current trace
if deflection_direction == 'up':
direction_input = True
else:
direction_input = False
##count function will return number of APs in trace and sample points for subsequent functions
trace_count, trace_sample_points_absolute = jjm_count(
start_msec,
delta_msec,
threshold=threshold_value,
up=direction_input,
trace=trace_selection,
mark=mark_option)
##finds afterdepolarizations--minimums between peaks
trace_ADP_values, trace_ADP_indicies = find_ADPs(
trace_sample_points_absolute)
trace_si = stf.get_sampling_interval()
trace_ADP_times = [sample * trace_si for sample in trace_ADP_indicies]
trace_AP_values, trace_AP_indicies = find_ADPs(
trace_sample_points_absolute)
trace_si = stf.get_sampling_interval()
trace_ADP_times = [sample * trace_si for sample in trace_AP_indicies]
trace_thresholds_indicies = find_thresholds(stf.get_trace(trace_selection),
trace_si, trace_ADP_indicies)
trace_threshold_values = [
stf.get_trace(trace_selection)[index]
for index in trace_thresholds_indicies
]
trace_threshold_times = [
sample * trace_si for sample in trace_thresholds_indicies
]
for sample, mv in zip(trace_thresholds_indicies, trace_threshold_values):
stf.set_marker(sample, mv)
for x in range(len(trace_threshold_values)):
if trace_threshold_values[
x] > threshold_value or trace_threshold_values[
x] < trace_ADP_values[x]:
trace_threshold_values[x] = 'NaN'
#arrays for output
ADP_out_array = np.transpose(np.array([trace_ADP_times, trace_ADP_values]))
threshold_out_array = np.transpose(
np.array([trace_threshold_times, trace_threshold_values]))
out_array = np.hstack([ADP_out_array, threshold_out_array])
df_out = pd.DataFrame(
out_array,
columns=['ADP time', 'ADP (mV)', 'threshold time', 'threshold (mV)'])
return (trace_count, df_out)
def count_events(start, delta, threshold=0, up=True, trace=None, mark=True):
"""
Counts the number of events (e.g action potentials (AP)) in the current trace.
Arguments:
start -- starting time (in ms) to look for events.
delta -- time interval (in ms) to look for events.
threshold -- (optional) detection threshold (default = 0).
up -- (optional) True (default) will look for upward events,
False downwards.
trace -- (optional) zero-based index of the trace in the current
channel, if None, the current trace is selected.
mark -- (optional) if True (default), set a mark at the point
of threshold crossing
Returns:
An integer with the number of events.
Examples:
count_events(500,1000) returns the number of events found between t=500
ms and t=1500 ms above 0 in the current trace and shows a stf
marker.
count_events(500,1000,0,False,-10,i) returns the number of events found
below -10 in the trace i and shows the corresponding stf markers.
"""
# sets the current trace or the one given in trace.
if trace is None:
sweep = stf.get_trace_index()
else:
if type(trace) !=int:
print('trace argument admits only integers')
return False
sweep = trace
# set the trace described in sweep
stf.set_trace(sweep)
# transform time into sampling points
dt = stf.get_sampling_interval()
pstart = int( round(start/dt) )
pdelta = int( round(delta/dt) )
# select the section of interest within the trace
selection = stf.get_trace()[pstart:(pstart+pdelta)]
# algorithm to detect events
event_counter, i = 0, 0 # set counter and index to zero
# choose comparator according to direction:
if up:
comp = lambda a, b: a > b
else:
comp = lambda a, b: a < b
# run the loop
while i < len(selection):
if comp(selection[i], threshold):
event_counter += 1
if mark:
stf.set_marker(pstart+i, selection[i])
while i < len(selection) and comp(selection[i], threshold):
i += 1 # skip until value is below/above threshold
else:
i += 1
return event_counter
