def _populate_iso(self, iso, layout):
StockSampleCreationIsoPopulator._populate_iso(self, iso, layout)
# Create sector preparation plates.
library_name = self.iso_request.label
ir_specs_96 = get_reservoir_specs_standard_96()
plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96)
ir_specs_384 = get_reservoir_specs_standard_384()
plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384)
future_status = get_item_status_future()
sec_layout_map = get_sector_layouts_for_384_layout(layout)
# Create preparation plates.
for sec_idx in range(NUMBER_SECTORS):
if not sec_idx in sec_layout_map:
continue
# TODO: Move label creation to LABELS class.
prep_label = self.PREP_PLATE_LABEL_PATTERN \
% (library_name,
iso.layout_number,
DEFAULT_PREPARATION_PLATE_CONCENTRATION,
sec_idx + 1)
prep_plate = plate_specs_96.create_rack(prep_label, future_status)
sec_layout = sec_layout_map[sec_idx]
iso.add_sector_preparation_plate(prep_plate, sec_idx,
sec_layout.create_rack_layout())
# Create aliquot plates.
for i in range(self.iso_request.number_aliquots):
# TODO: Move label creation to LABELS class.
aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN \
% (library_name,
iso.layout_number,
DEFAULT_ALIQUOT_PLATE_CONCENTRATION,
i + 1)
aliquot_plate = plate_specs_384.create_rack(aliquot_label,
future_status)
iso.add_aliquot_plate(aliquot_plate)
def __init__(self, iso_request, number_isos, **kw):
StockSampleCreationIsoPopulator.__init__(self, iso_request,
number_isos, **kw)
#: The library base layout.
self.__base_layout = None
#: Maps sector indices -> positions.
self.__sector_positions = None
def reset(self):
StockSampleCreationIsoPopulator.reset(self)
self.__base_layout = None
self.__sector_positions = None