def run(path, f, output_path, dictionary, column='gene', **kwargs):
"""Run and save e.a.."""
genes = pd.read_csv(path+f, **kwargs)
df = tea.enrichment_analysis(genes[column], dictionary,
show=False)
df = df[df.Observed > 2]
df.to_csv(output_path + f + '.csv', index=False)
def run_tea(parent, sleuth, out, q_threshold=0.05):
"""
Runs TEA on sleuth output.
"""
try:
import tissue_enrichment_analysis as tea
except ImportError as e:
print_with_flush('# TEA is not installed...skipping')
sys.exit(0)
try:
import pandas as pd
except ImportError as e:
print_with_flush('# pandas is not installed...skipping')
sys.exit(0)
analyses = ['tissue', 'phenotype', 'go']
# Load sleuth results.
wdir = os.getcwd()
print_with_flush('# entering 3_diff_exp')
os.chdir(parent)
print_with_flush('# creating {} directory'.format(out))
os.makedirs(out, exist_ok=True)
for file in os.listdir(sleuth):
if file.endswith('.csv'):
df = pd.read_csv(os.path.join(sleuth, file), index_col=0)
gene_list = df[df.qval < q_threshold].ens_gene
name = os.path.splitext(os.path.basename(file))[0]
if len(gene_list) == 0:
print_with_flush(('# there are no genes with q < {} in ' +
'{}!').format(q_threshold, file))
print_with_flush(
'# this means there are no significantly ' +
'differentially-expressed genes for ' +
'this set of conditions.')
continue
for analysis in analyses:
print_with_flush(('# performing {} enrichment analysis ' +
'for {}').format(analysis, file))
fname = '{}_{}'.format(name.replace('betas_wt', out),
analysis)
title = os.path.join(out, fname)
df_dict = tea.fetch_dictionary(analysis)
df_results = tea.enrichment_analysis(gene_list,
df_dict,
aname=title + '.csv',
save=True,
show=False)
tea.plot_enrichment_results(df_results,
analysis=analysis,
title=title,
save=True)
os.chdir(wdir)
print_with_flush('# returned to root')
def walker(tissue_df, directory, save=True):
"""Given the tissue dictionary and a directory to save to,
open all the gene sets, analyze them and deposit the results in the
specified directory.
Parameters:
-------------------
tissue_df - pandas dataframe containing specified tissue dictionary
directory - where to save to
save - boolean indicating whether to save results or not.
"""
with open(directory + 'empty.txt', 'w') as f:
f.write('Genesets with no enrichment:\n')
# go through each file in the folder
for fodder in os.walk(path_sets):
for f_set in fodder[2]:
# open df
df = pd.read_csv(path_sets + f_set)
# extract gene list and analyze
short_name = f_set
test = df.gene.values
df_analysis, unused = tea.enrichment_analysis(test,
tissue_df,
show=False)
# if it's not empty and you want to save:
if df_analysis.empty is False & save:
# save without index
df_analysis.to_csv(directory + short_name + '.csv',
index=False)
# add a comment
line = '#' + short_name + '\n'
line_prepender(directory + short_name + '.csv', line)
# plot
tea.plot_enrichment_results(df_analysis,
title=short_name,
dirGraphs=directory,
ftype='pdf')
plt.close()
# if it's empty and you want to save, place it in file called empty
if df_analysis.empty & save:
with open(directory + 'empty.txt', 'a+') as f:
f.write(short_name + '\n')
def walker(tissue_df, directory, save=True):
"""Given the tissue dictionary and a directory to save to,
open all the gene sets, analyze them and deposit the results in the
specified directory.
Parameters:
-------------------
tissue_df - pandas dataframe containing specified tissue dictionary
directory - where to save to
save - boolean indicating whether to save results or not.
"""
with open(directory+'empty.txt', 'w') as f:
f.write('Genesets with no enrichment:\n')
# go through each file in the folder
for fodder in os.walk(path_sets):
for f_set in fodder[2]:
# open df
df = pd.read_csv(path_sets + f_set)
# extract gene list and analyze
short_name = f_set
test = df.gene.values
df_analysis, unused = tea.enrichment_analysis(test, tissue_df,
show=False)
# if it's not empty and you want to save:
if df_analysis.empty is False & save:
# save without index
df_analysis.to_csv(directory+short_name+'.csv', index=False)
# add a comment
line = '#' + short_name+'\n'
line_prepender(directory+short_name+'.csv', line)
# plot
tea.plot_enrichment_results(df_analysis, title=short_name,
dirGraphs=directory, ftype='pdf')
plt.close()
# if it's empty and you want to save, place it in file called empty
if df_analysis.empty & save:
with open(directory+'empty.txt', 'a+') as f:
f.write(short_name+'\n')
thresh = float(thresh) # typecasting
method = f_dict[-7:-4]
ntiss = len(tissue_df.columns)
ngenes = tissue_df.shape[0]
# open each enrichment set
for fodder in os.walk(path_sets):
for f_set in fodder[2]:
df = pd.read_csv(path_sets + f_set)
test = df.gene.values
ntest = len(test)
short_name = f_set[16:len(f_set)-16]
df_analysis, unused = tea.enrichment_analysis(test, tissue_df,
alpha=0.05,
show=False)
nana = len(df_analysis) # len of results
nun = len(unused) # number of genes dropped
avf = df_analysis['Fold Change'].mean()
avq = df_analysis['Q value'].mean()
s = '{0},{1},{2},{3},{4},{5},{6},{7},{8},{9},{10}'.format(
annot, thresh, method, f_set, ntiss, ntest, nana,
ntest-nun, avf, avq, ngenes)
with open(dirSummaries+'ExecutiveSummary.csv', 'a+') as fSum:
fSum.write(s)
fSum.write('\n')
# Print summary to csv
df_summary = pd.read_csv(dirSummaries+'ExecutiveSummary.csv', comment='#')
for i, df in enumerate(Ldf):
fname = Lnames[i]
obj = receptacle(fname)
for direction in Ldirection:
ind = g(df[df[direction] == 1.0], 'SequenceNameGene')
x = names[ind].WBID
print('---------')
print(fname + ' ' + direction)
print('Number of genes submitted for analysis ', len(x))
y = tissue_df[tissue_df.wbid.isin(x)].wbid.unique().shape[0]
print('Number of genes used for analysis ', y)
print('\n')
df_res, unused = tea.enrichment_analysis(x, tissue_df,
show=False, alpha=0.1)
print(df_res.empty)
if len(df_res) > 0:
obj.add_result(direction, df_res)
n_genes.append(['{0}, {1}'.format(fname, direction), y, len(unused)])
Lreceptacles[fname] = obj
# make
for species in Lreceptacles:
current = Lreceptacles[species]
n = current.n # number of dfs
def run(path, f, output_path, dictionary, **kwargs):
"""Run and save e.a.."""
genes = pd.read_csv(path+f, **kwargs)
df, _ = tea.enrichment_analysis(genes.gene, dictionary,
show=False)
df.to_csv(output_path + f + '.csv', index=False)
print('-----------------------------------')
print('Genes with annotated phenotype terms: ',
get_n(pheno_traits, worm_genes))
print('Genes with annotated tissue terms: ',
get_n(tissue_traits, worm_genes))
print('Genes with annotated go terms: ',
get_n(go_traits, worm_genes))
print('-----------------------------------\n')
# one of the traits foolishly has a '/'
if '/' in trait:
# rename the trait with this character or it breaks your code
trait = 'post bronchodilator fev1 fevc ratio'
# enrichment analyses:
df = tea.enrichment_analysis(worm_genes, phenotype_df, show=False)
df = df[df.Observed > n_min_obs].copy()
df.to_csv('../output/phenologues_2/pea_' + trait + '.csv', index=False)
if 'lupus' in trait:
print('Graphing PEA results for ', trait)
fig, ax = plt.subplots()
tea.plot_enrichment_results(df, title='../output/lupus_pea',
save=True, analysis='phenotype')
plt.close()
df = tea.enrichment_analysis(worm_genes, tissue_df, show=False)
df = df[df.Observed > n_min_obs].copy()
df.to_csv('../output/disease_tissues_2/tea_' + trait + '.csv',
index=False)
if 'lupus' in trait:
print('Graphing TEA results for ', trait)
import tissue_enrichment_analysis as tea
import pandas as pd
phenotype_df = pd.read_csv('../input/phenotype_ontology.csv')
go_df = pd.read_csv('../input/gene_ontology.csv')
tissue_df = tea.fetch_dictionary()
sfari = pd.read_excel('../input/sfari.xlsx')
name_df = pd.read_excel('../input/sfari_name_converter.xlsx')
sfari.head()
df, _ = tea.enrichment_analysis(sfari.Gene, tissue_df, show=False)
df.to_csv('../output/tea_sfari.csv', index=False)
df, _ = tea.enrichment_analysis(sfari.Gene, phenotype_df, show=False)
df.to_csv('../output/pea_sfari.csv', index=False)
df, _ = tea.enrichment_analysis(sfari.Gene, go_df, show=False)
df.to_csv('../output/goa_sfari.csv', index=False)
melt_pheno = pd.melt(phenotype_df, id_vars='wbid', var_name='phenotype')
melt_pheno = melt_pheno[melt_pheno.value == 1]
def convert(x):
return name_df[name_df.wbid == x].gene_name.values[0]
def go_enrichment(self,
mode: str = 'go',
alpha: float = 0.05,
save_csv: bool = False,
fname: str = None):
"""
Analyzes GO, Tissue and/or Phenotype enrichment of the given group of features. \
Uses the the Anatomy, Phenotype and Gene Ontology annotations for C. elegans. \
Corrected p-values are calculated using hypergeometric statistics. \
For more details see GitHub page of the developers: https://github.com/dangeles/TissueEnrichmentAnalysis
:param mode: the enrichment you wish to perform. 'go' for gene ontology enrichment, \
'tissue' for tissue enrichment, 'phenotype' for phenotype enrichment.
:param alpha: float. Significance threshold. Default is 0.05
:param save_csv: bool. False by default. If True, save the result to a csv.
:param fname: Name and path in which to save the results. Must be filled if save_csv is True.
:return:
a DataFrame which contains the significant enrichmenet terms
.. figure:: go_en.png
:align: center
:scale: 40 %
Example plot of GO enrichment
.. figure:: tissue_en.png
:align: center
:scale: 40 %
Example plot of Tissue enrichment
"""
assert isinstance(alpha, float), "alpha must be a float!"
assert isinstance(mode, str), "'mode' must be a string!"
if mode == 'all':
d = []
df_comb = pd.DataFrame()
for k, arg in enumerate(('go', 'tissue', 'phenotype')):
print(f'Calculating... {100 * k / 3 :.2f}% done')
if arg in EnrichmentProcessing._go_dicts:
d.append(EnrichmentProcessing._go_dicts[arg])
else:
d.append(tea.fetch_dictionary(arg))
EnrichmentProcessing._go_dicts[arg] = d[-1]
df = tea.enrichment_analysis(self.gene_set, d[-1], alpha=alpha)
df_comb = df_comb.append(df)
tea._plot_enrichment_results(
df,
title=f'{arg.capitalize()} Enrichment Analysis',
analysis=arg)
plt.title(f'{arg.capitalize()} Enrichment Analysis',
fontsize=20)
print(df)
else:
assert (mode == 'go' or mode == 'tissue'
or mode == 'phenotype'), "Invalid mode!"
d = tea.fetch_dictionary(mode)
df_comb = tea.enrichment_analysis(self.gene_set, d, show=True)
tea._plot_enrichment_results(
df_comb,
title=f'{mode.capitalize()} Enrichment Analysis',
analysis=mode)
plt.title(f'{mode.capitalize()} Enrichment Analysis', fontsize=20)
if save_csv:
self._enrichment_save_csv(df_comb, fname)
plt.show()
return df_comb
thresh = float(thresh) # typecasting
method = f_dict[-7:-4]
ntiss = len(tissue_df.columns)
ngenes = tissue_df.shape[0]
# open each enrichment set
for fodder in os.walk(path_sets):
for f_set in fodder[2]:
df = pd.read_csv(path_sets + f_set)
test = df.gene.values
ntest = len(test)
short_name = f_set[16:len(f_set) - 16]
df_analysis, unused = tea.enrichment_analysis(test,
tissue_df,
alpha=0.05,
show=False)
# save the analysis to the relevant folder
savepath = '../output/HGT' + annot + '_' + method + '_Results/'
df_analysis.to_csv(savepath + f_set + '.csv', index=False)
tea.plot_enrichment_results(df_analysis,
save='savepath' + f_set + 'Graph',
ftype='pdf')
nana = len(df_analysis) # len of results
nun = len(unused) # number of genes dropped
avf = df_analysis['Enrichment Fold Change'].mean()
avq = df_analysis['Q value'].mean()
s = '{0},{1},{2},{3},{4},{5},{6},{7},{8},{9},{10}'.format(
def run_post(project, code='post', requires='diff'):
print_with_flush('# starting post for project {}'.format(project.objectId))
organism = project.relation('samples').query()[0].reference.organism
if organism.genus != 'caenorhabditis' or organism.species != 'elegans':
print_with_flush('# Currently, post analysis is only supported for '
'C. elegans')
return
config = Config.get()
q_threshold = config['qThreshold']
tea_types = config['teaTypes']
diff_path = project.paths[requires]
post_path = project.paths[code]
for file in os.listdir(diff_path):
file_name = os.path.splitext(os.path.basename(file))[0]
file_path = os.path.join(diff_path, file)
if file.startswith('betas') and file.endswith('.csv'):
df = pd.read_csv(file_path, index_col=0)
gene_list = df[df.qval < q_threshold].ens_gene
# Skip if gene list is empty.
if len(gene_list) == 0:
print_with_flush(
('# there are no genes with q < {} in ' + '{}!').format(
q_threshold, file))
print_with_flush('# this means there are no significantly ' +
'differentially-expressed genes for ' +
'this set of conditions.')
continue
for tea_type in tea_types:
tea_file = '{}_{}'.format(
file_name.replace('betas_wt', 'enrichment'), tea_type)
tea_title = os.path.join(post_path, tea_file)
print_with_flush(
('# performing {} enrichment analysis ' + 'for {}').format(
tea_type, file))
df_dict = tea.fetch_dictionary(tea_type)
df_results = tea.enrichment_analysis(gene_list,
df_dict,
aname=tea_title + '.csv',
save=True,
show=False)
tea.plot_enrichment_results(df_results,
analysis=tea_type,
title=tea_title,
save=True)
# Archive.
archive_path = archive(project, code)
if code not in project.files:
project.files[code] = {}
project.files[code]['archive'] = archive_path
project.save()
print_with_flush('# done')
