Esempio n. 1
0
 def test_output006(self):
     """Check output006.m10 file."""
     fasta_file = "Fasta/output006.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 1)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 131)
         self.assertEqual(
             alignments[0][0].seq,
             "GCAACGCTTCAAGAACTGGAATT"
             "AGGAACCGTGACAACGATTAATG"
             "AGGAGATTTATGAAGAGGGTTCT"
             "TCGATTTTAGGCCAATCGGAAGG"
             "AATTATGTAGCAAGTCCATCAGA"
             "AAATGGAAGAAGTCAT",
         )
         self.assertEqual(alignments[0][0].id, "query")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          131)
         self.assertEqual(
             alignments[0][1].seq,
             "GCAACGCTTCAAGAACTGGAATT"
             "AGGAACCGTGACAACGATTAATG"
             "AGGAGATTTATGAAGAGGGTTCT"
             "TCGATTTTAGGCCAATCGGAAGG"
             "AATTATGTAGCAAGTCCATCAGA"
             "AAATGGAAGTAGTCAT",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|116660610|gb|EG558221.1|EG558221")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          573)
Esempio n. 2
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 def test_output005(self):
     """Check output005.m10 file."""
     fasta_file = "Fasta/output005.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 1)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 110)
         self.assertEqual(
             alignments[0][0].seq,
             "IKNKDKTLFIVYAT-DIYSPSEF"
             "FSKIESDLKKKKSKGDV--FFDL"
             "IIPNGGKKD--RYVYTSFNGEKF"
             "SSYTLNKVTKTDEYNDL--SELS"
             "ASFFKKNFDKINVNLLSK",
         )
         self.assertEqual(alignments[0][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          126)
         self.assertEqual(
             alignments[0][1].seq,
             "IKDELPVAFCSWASLDLECEVKY"
             "INDVTSLYAKDWMSGERKWFIDW"
             "IAPFGHNMELYKYMRKKYPYELF"
             "RAIRLDESSKTGKIAEFHGGGID"
             "KKLASKIFRQYHHELMSE",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|10955282|ref|NP_052623.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          163)
Esempio n. 3
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 def test_output004(self):
     """Check output004.m10 file."""
     fasta_file = "Fasta/output004.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 1)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 102)
         self.assertEqual(
             alignments[0][0].seq,
             "AAAAAAGATAAAAAATATCAAAT"
             "AGAAGCAATAAAAAATAAAGATA"
             "AAACTTTATTTATTGTCTATGCT"
             "ACTGATATTTATAGCCCGAGCGA"
             "ATTTTTCTCA",
         )
         self.assertEqual(alignments[0][0].id, "ref|NC_002127.1|:c1351-971")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          381)
         self.assertEqual(
             alignments[0][1].seq,
             "AGAGAAAATAAAACAAGTAATAA"
             "AATATTAATGGAAAAAATAAATT"
             "CTTGTTTATTTAGACCTGATTCT"
             "AATCACTTTTCTTGCCCGGAGTC"
             "ATTTTTGACA",
         )
         self.assertEqual(alignments[0][1].id,
                          "ref|NC_002695.1|:1970775-1971404")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          630)
Esempio n. 4
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 def test_output003(self):
     """Check output003.m10 file."""
     fasta_file = "Fasta/output003.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 3)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 55)
         self.assertEqual(
             alignments[0][0].seq,
             "ISISNNKDQYEELQKEQGERDLKTVDQLVRIAAAGGGLRLSASTKTVDQLVRIAA",
         )
         self.assertEqual(alignments[0][0].id,
                          "gi|152973837|ref|YP_001338874.1|")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          183)
         self.assertEqual(
             alignments[0][1].seq,
             "VRLTAEEDQ--EIRKRAAECG-KTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGA",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          107)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 22)
         self.assertEqual(alignments[1][0].seq, "DDAEHLFRTLSSR-LDALQDGN")
         self.assertEqual(alignments[1][0].id,
                          "gi|152973840|ref|YP_001338877.1|")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          63)
         self.assertEqual(alignments[1][1].seq, "DDRANLFEFLSEEGITITEDNN")
         self.assertEqual(alignments[1][1].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          346)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 63)
         self.assertEqual(
             alignments[2][0].seq,
             "VFGSFEQPKGEHLSGQVSEQ--RDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDSLKQAM",
         )
         self.assertEqual(alignments[2][0].id,
                          "gi|152973841|ref|YP_001338878.1|")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          133)
         self.assertEqual(
             alignments[2][1].seq,
             "VYTSFN---GEKFSSYTLNKVTKTDEYNDLSELSASFFKKNFDKINVNLLSKATSF-ALKKGI",
         )
         self.assertEqual(alignments[2][1].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          126)
Esempio n. 5
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 def test_output009(self):
     """Check output009.m10 file."""
     fasta_file = "Fasta/output009.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 7)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 22)
         self.assertEqual(alignments[0][0].seq, "TGATGTTCTGTTTCTAAAACAG")
         self.assertEqual(alignments[0][0].id, "gi|255708421:1-99")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          99)
         self.assertEqual(alignments[0][1].seq, "TGATTTTTTTTGTCTAAAACAG")
         self.assertEqual(alignments[0][1].id,
                          "gi|23308614|ref|NM_152952.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          5188)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 14)
         self.assertEqual(alignments[1][0].seq, "AGAAGGAAAAAAAA")
         self.assertEqual(alignments[1][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[1][1].seq, "AGAACTAAAAAAAA")
         self.assertEqual(alignments[1][1].id,
                          "gi|47271416|ref|NM_131257.2|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          597)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 14)
         self.assertEqual(alignments[2][0].seq, "AGAAGGAAAAAAAA")
         self.assertEqual(alignments[2][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[2][1].seq, "AGAAGGTATAAAAA")
         self.assertEqual(alignments[2][1].id,
                          "gi|332859474|ref|XM_001156938.2|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          762)
         self.assertEqual(len(alignments[3]), 2)
         self.assertEqual(alignments[3].get_alignment_length(), 14)
         self.assertEqual(alignments[3][0].seq, "TTTTTTTCCTTCTT")
         self.assertEqual(alignments[3][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[3][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[3][1].seq, "TTTTTTTACATCTT")
         self.assertEqual(alignments[3][1].id,
                          "gi|332211534|ref|XM_003254825.1|")
         self.assertEqual(alignments[3][1].annotations["original_length"],
                          805)
         self.assertEqual(len(alignments[4]), 2)
         self.assertEqual(alignments[4].get_alignment_length(), 14)
         self.assertEqual(alignments[4][0].seq, "AAGAAGGAAAAAAA")
         self.assertEqual(alignments[4][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[4][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[4][1].seq, "AATAAGTAAAAAAA")
         self.assertEqual(alignments[4][1].id,
                          "gi|23308614|ref|NM_152952.1|")
         self.assertEqual(alignments[4][1].annotations["original_length"],
                          5188)
         self.assertEqual(len(alignments[5]), 2)
         self.assertEqual(alignments[5].get_alignment_length(), 14)
         self.assertEqual(alignments[5][0].seq, "TTTTTTTCCTTCTT")
         self.assertEqual(alignments[5][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[5][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[5][1].seq, "TTTTTTTACATCTT")
         self.assertEqual(alignments[5][1].id,
                          "gi|297689475|ref|XM_002822130.1|")
         self.assertEqual(alignments[5][1].annotations["original_length"],
                          1158)
         self.assertEqual(len(alignments[6]), 2)
         self.assertEqual(alignments[6].get_alignment_length(), 14)
         self.assertEqual(alignments[6][0].seq, "AAGAAGGAAAAAAA")
         self.assertEqual(alignments[6][0].id, "gi|156718121:2361-2376")
         self.assertEqual(alignments[6][0].annotations["original_length"],
                          16)
         self.assertEqual(alignments[6][1].seq, "AAGAAGGTAAAAGA")
         self.assertEqual(alignments[6][1].id,
                          "gi|297689475|ref|XM_002822130.1|")
         self.assertEqual(alignments[6][1].annotations["original_length"],
                          1158)
Esempio n. 6
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 def test_output002(self):
     """Check output002.m10 file."""
     fasta_file = "Fasta/output002.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 6)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 88)
         self.assertEqual(
             alignments[0][0].seq,
             "SGSNTRRRAISRPVR--LTAEED"
             "QEIRKRAAECG-KTVSGFLRAAA"
             "LGKKVNSLTDDRVLKEVMRLGAL"
             "QKKLFIDGKRVGDREYAEV",
         )
         self.assertEqual(alignments[0][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[0][1].seq,
             "SQRSTRRKPENQPTRVILFNKPY"
             "DVLPQFTDEAGRKTLKEFIPVQG"
             "VYAAGRLDRDSEGLLVLTNNGAL"
             "QARLTQPGKRTGKIYYVQV",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|162139799|ref|NP_309634.2|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          207)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 53)
         self.assertEqual(
             alignments[1][0].seq,
             "EIRKRAAECGKTVSGFLRAAA-LGKKV----NSLTDDRVLKEVMRLGALQKKL",
         )
         self.assertEqual(alignments[1][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[1][1].seq,
             "EIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKI",
         )
         self.assertEqual(alignments[1][1].id,
                          "gi|15831859|ref|NP_310632.1|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          266)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 92)
         self.assertEqual(
             alignments[2][0].seq,
             "SEFFSKIESDLKKKKSKGDVFFD"
             "LIIPNG-----GKKDRYVYTSFN"
             "GEKFSSYTLNKVTKTDEYNDLSE"
             "LSASFFKKNFDKINVNLLSKATS",
         )
         self.assertEqual(alignments[2][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          126)
         self.assertEqual(
             alignments[2][1].seq,
             "TELNSELAKAMKVDAQRG-AFVS"
             "QVLPNSSAAKAGIKAGDVITSL"
             "NGKPISSFAALRA-QVGTMPVG"
             "SKLTLGLLRDG-KQVNVNLELQ"
             "QSS",
         )
         self.assertEqual(alignments[2][1].id,
                          "gi|15829419|ref|NP_308192.1|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          474)
         self.assertEqual(len(alignments[3]), 2)
         self.assertEqual(alignments[3].get_alignment_length(), 73)
         self.assertEqual(
             alignments[3][0].seq,
             "FFDLIIPNGGKKDRYVYTSFNGE"
             "KFSSYTLNKVTKTDEYNDLSELS"
             "ASFFKKNFDKINVNLLSKATSFA"
             "LKKG",
         )
         self.assertEqual(alignments[3][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[3][0].annotations["original_length"],
                          126)
         self.assertEqual(
             alignments[3][1].seq,
             "LFDLFLKNDAMHDPMVNESYC-E"
             "TFGWVSKENLARMKE---LTYKA"
             "NDVLKKLFDDAGLILVDFKLEFG"
             "LYKG",
         )
         self.assertEqual(alignments[3][1].id,
                          "gi|15832592|ref|NP_311365.1|")
         self.assertEqual(alignments[3][1].annotations["original_length"],
                          237)
         self.assertEqual(len(alignments[4]), 2)
         self.assertEqual(alignments[4].get_alignment_length(), 63)
         self.assertEqual(
             alignments[4][0].seq,
             "VDIKK-ETIESELHSKLPKSIDKIHEDIKKQLSCSLI--MKKID-VEMEDYSTYCFSALRAIE",
         )
         self.assertEqual(alignments[4][0].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[4][0].annotations["original_length"],
                          346)
         self.assertEqual(
             alignments[4][1].seq,
             "IDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTRE",
         )
         self.assertEqual(alignments[4][1].id,
                          "gi|38704138|ref|NP_311957.2|")
         self.assertEqual(alignments[4][1].annotations["original_length"],
                          111)
         self.assertEqual(len(alignments[5]), 2)
         self.assertEqual(alignments[5].get_alignment_length(), 157)
         self.assertEqual(
             alignments[5][0].seq,
             "QYIMTTSNGDRVRAKIYKRGSIQ"
             "FQGKYLQIASLINDFMCSILNMK"
             "EIVEQKNKEFNVDI---KKETI-"
             "ESELHSKLPKSIDKIHEDIKKQL"
             "SCSLIMKKIDV-EMEDYSTYCFS"
             "ALRA-IEGFIYQILNDVCNPSSS"
             "KNLGEYFTENKPKYIIREI",
         )
         self.assertEqual(alignments[5][0].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[5][0].annotations["original_length"],
                          346)
         self.assertEqual(
             alignments[5][1].seq,
             "EFIRLLSDHDQFEKDQISELTVA"
             "ANALKLEVAK--NNY-----NMK"
             "YSFDTQTERRMIELIREQKDLIP"
             "EKYLHQSGIKKL-KLHED---EF"
             "SSLLVDAERQVLEGSSFVLCCGE"
             "KINSTISELLSKKITDLTHPTES"
             "FTLSEYFSYDVYEEIFKKV",
         )
         self.assertEqual(alignments[5][1].id,
                          "gi|15833861|ref|NP_312634.1|")
         self.assertEqual(alignments[5][1].annotations["original_length"],
                          330)
Esempio n. 7
0
 def test_output008(self):
     """Check output008.m10 file."""
     fasta_file = "Fasta/output008.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 12)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 65)
         self.assertEqual(
             alignments[0][0].seq,
             "LQHRHPHQQQQQQQQQQQQQQQQ"
             "QQQQQQQQQQQH---HHHHHHHL"
             "LQDAYMQQYQHATQQQQML",
         )
         self.assertEqual(alignments[0][0].id, "sp|Q9NSY1|BMP2K_HUMAN")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          1161)
         self.assertEqual(
             alignments[0][1].seq,
             "IPHQLPHALRHRPAQEAAHASQL"
             "HPAQPGCGQPLHGLWRLHHHPVY"
             "LYAWILRLRGHGMQSGGLL",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|283855822|gb|GQ290312.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          983)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 201)
         self.assertEqual(
             alignments[1][0].seq,
             "GPEIL---LGQ-GPPQQPPQQHR"
             "VLQQLQQGDWRLQQLH-------"
             "LQHRHPHQQQQQQQQQQQQQQQQ"
             "QQQQQQQQQQQH-----HHHHHH"
             "-HLLQDAYMQQYQHATQQQQMLQ"
             "QQF-LMHSVYQPQPSASQYPTMM"
             "PQYQQAFFQQQMLAQHQPSQQQA"
             "SPEYLTSPQEFSPALVSYTSSLP"
             "A-QVGTIMDSSYSANRS",
         )
         self.assertEqual(alignments[1][0].id, "sp|Q9NSY1|BMP2K_HUMAN")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          1161)
         self.assertEqual(
             alignments[1][1].seq,
             "GPELLRALLQQNGCGTQPLRVPT"
             "VLPG*AMAVLHAGRLHVPAHRAW"
             "LPHQLPHALRHGPAQEAAHASQL"
             "HPAQPGRG*PLHGLRWLHHHPLH"
             "/PLCMDTLSLGPQDAIWRASLPH"
             "WAVKLPCGLWWSWPLSGTWWCVS"
             "P*ATSA------LGRTMP*WASL"
             "SPGSWHWPALHPPSLVGPGTSLK"
             "ACSVHAGSTTTHSSQKS",
         )
         self.assertEqual(alignments[1][1].id,
                          "gi|57163782|ref|NM_001009242.1|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          1047)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 348)
         self.assertEqual(
             alignments[2][0].seq,
             "MNGTEGPNFYVPFSNATGVVRSP"
             "FEYPQYYLAEPWQFSMLAAYMFL"
             "LIVLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVLGGFT"
             "STLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLAGWSRYIPEGLQ"
             "CSCGIDYYTLKPEVNNESFVIYM"
             "FVVHFTIPMIIIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWVPYASVAFYIF"
             "THQGSNFGPIFMTIPAFFAKSAA"
             "IYNPVIYIMMNKQFRNCMLTTIC"
             "CGKNPLGDDEASATVSKTETSQV"
             "APA",
         )
         self.assertEqual(alignments[2][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[2][1].seq,
             "MNGTEGPNFYVPFSNKTGVVRSP"
             "FEYPQYYLAEPWQFSMLAAYMFL"
             "LIVLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVFGGFT"
             "TTLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLVGWSRYIPEGMQ"
             "CSCGIDYYTLKPEVNNESFVIYM"
             "FVVHFTIPMIVIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWVPYASVAFYIF"
             "THQGSNFGPIFMTLPAFFAKSSS"
             "IYNPVIYIMMNKQFRNCMLTTLC"
             "CGKNPLGDDEASTTGSKTETSQV"
             "APA",
         )
         self.assertEqual(alignments[2][1].id,
                          "gi|57163782|ref|NM_001009242.1|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          1047)
         self.assertEqual(len(alignments[3]), 2)
         self.assertEqual(alignments[3].get_alignment_length(), 348)
         self.assertEqual(
             alignments[3][0].seq,
             "MNGTEGPNFYVPFSNATGVVRSP"
             "FEYPQYYLAEPWQFSMLAAYMFL"
             "LIVLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVLGGFT"
             "STLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLAGWSRYIPEGLQ"
             "CSCGIDYYTLKPEVNNESFVIYM"
             "FVVHFTIPMIIIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWVPYASVAFYIF"
             "THQGSNFGPIFMTIPAFFAKSAA"
             "IYNPVIYIMMNKQFRNCMLTTIC"
             "CGKNPLGDDEASATVSKTETSQV"
             "APA",
         )
         self.assertEqual(alignments[3][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[3][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[3][1].seq,
             "MNGTEGPNFYVPFSNKTGVVRSP"
             "FEAPQYYLAEPWQFSMLAAYMFL"
             "LIMLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVFGGFT"
             "TTLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLVGWSRYIPEGMQ"
             "CSCGIDYYTPHEETNNESFVIYM"
             "FVVHFIIPLIVIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWLPYAGVAFYIF"
             "THQGSDFGPIFMTIPAFFAKTSA"
             "VYNPVIYIMMNKQFRNCMVTTLC"
             "CGKNPLGDDEASTTVSKTETSQV"
             "APA",
         )
         self.assertEqual(alignments[3][1].id,
                          "gi|18148870|dbj|AB062417.1|")
         self.assertEqual(alignments[3][1].annotations["original_length"],
                          1047)
         self.assertEqual(len(alignments[4]), 2)
         self.assertEqual(alignments[4].get_alignment_length(), 326)
         self.assertEqual(
             alignments[4][0].seq,
             "VPFSNATGVVRSPFEYPQYYLAE"
             "PWQFSMLAAYMFLLIVLGFPINF"
             "LTLYVTVQHKKLRTPLNYILLNL"
             "AVADLFMVLGGFTSTLYTSLHGY"
             "FVFGPTGCNLEGFFATLGGEIAL"
             "WSLVVLAIERYVVVCKPMSNFRF"
             "GENHAIMGVAFTWVMALACAAPP"
             "LAGWSRYIPEGLQCSCGIDYYTL"
             "KPEVNNESFVIYMFVVHFTIPMI"
             "IIFFCYGQLVFTVKEAAAQQQES"
             "ATTQKAEKEVTRMVIIMVIAFLI"
             "CWVPYASVAFYIFTHQGSNFGPI"
             "FMTIPAFFAKSAAIYNPVIYIMM"
             "NKQFRNCMLTTICCGKNPLGDDE"
             "ASAT",
         )
         self.assertEqual(alignments[4][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[4][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[4][1].seq,
             "VPFSNKTGVVRSPFEYPQYYLAE"
             "PWQFSMLAAYMFLLIVLGFPINF"
             "LTLYVTVQHKKLRTPLNYILLNL"
             "AVANLFMVFGGFTTTLYTSMHGY"
             "FVFGATGCNLEGFFATLGGEIAL"
             "WSLVVLAIERYVVVCKPMSNFRF"
             "GENHAIMGLAFTWVMALACAAPP"
             "LAGWSRYIPEGMQCSCGIDYYTL"
             "KPEVNNESFVIYMFVVHFTIPMI"
             "VIFFCYGQLVFTVKEAAAQQQES"
             "ATTQKAEKEVTRMVIIMVVAFLI"
             "CWLPYASVAFYIFTHQGSNFGPV"
             "FMTIPAFFAKSSSIYNPVIYIMM"
             "NKQFRNCMLTTLCCGKNPLGDDE"
             "ASTT",
         )
         self.assertEqual(alignments[4][1].id,
                          "gi|283855822|gb|GQ290312.1|")
         self.assertEqual(alignments[4][1].annotations["original_length"],
                          983)
         self.assertEqual(len(alignments[5]), 2)
         self.assertEqual(alignments[5].get_alignment_length(), 354)
         self.assertEqual(
             alignments[5][0].seq,
             "MNGTEGPNFYVPFSNATGVVRSP"
             "FEYPQYYLAEPWQFSMLAAYMFL"
             "LIVLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVLGGFT"
             "STLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLAGWSRYIPEGLQ"
             "CSCGIDYYTLKPEVNNESFVIYM"
             "FVVHFTIPMIIIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWVPYASVAFYIF"
             "THQGSNFGPIFMTIPAFFAKSAA"
             "IYNPVIYIMMNKQFRNCMLTTIC"
             "CGKNPLGDDEAS-ATVSKTE---"
             "--TSQVAPA",
         )
         self.assertEqual(alignments[5][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[5][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[5][1].seq,
             "MNGTEGPNFYIPMSNKTGVVRSP"
             "FEYPQYYLAEPWQYSILCAYMFL"
             "LILLGFPINFMTLYVTIQHKKLR"
             "TPLNYILLNLAFANHFMVLCGFT"
             "VTMYSSMNGYFILGATGCYVEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFSENHAVMGVAFTW"
             "IMALSCAVPPLLGWSRYIPEGMQ"
             "CSCGVDYYTLKPEVNNESFVIYM"
             "FVVHFTIPLIIIFFCYGRLVCTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVVFFLICWVPYASVAFFIF"
             "SNQGSEFGPIFMTVPAFFAKSSS"
             "IYNPVIYIMLNKQFRNCMITTLC"
             "CGKNPFGEDDASSAATSKTEASS"
             "VSSSQVSPA",
         )
         self.assertEqual(alignments[5][1].id,
                          "gi|2734705|gb|U59921.1|BBU59921")
         self.assertEqual(alignments[5][1].annotations["original_length"],
                          1574)
         self.assertEqual(len(alignments[6]), 2)
         self.assertEqual(alignments[6].get_alignment_length(), 347)
         self.assertEqual(
             alignments[6][0].seq,
             "MNGTEGPNFYVPFSNATGVVRSP"
             "FEYPQYYLAEPWQFSMLAAYMFL"
             "LIVLGFPINFLTLYVTVQHKKLR"
             "TPLNYILLNLAVADLFMVLGGFT"
             "STLYTSLHGYFVFGPTGCNLEGF"
             "FATLGGEIALWSLVVLAIERYVV"
             "VCKPMSNFRFGENHAIMGVAFTW"
             "VMALACAAPPLAGWSRYIPEGLQ"
             "CSCGIDYYTLKPEVNNESFVIYM"
             "FVVHFTIPMIIIFFCYGQLVFTV"
             "KEAAAQQQESATTQKAEKEVTRM"
             "VIIMVIAFLICWVPYASVAFYIF"
             "THQGSNFGPIFMTIPAFFAKSAA"
             "IYNPVIYIMMNKQFRNCMLTTIC"
             "CGKNPLGD-DEASATVSKTETSQ"
             "VA",
         )
         self.assertEqual(alignments[6][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[6][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[6][1].seq,
             "MNGTEGPNFYIPMSNATGVVRSP"
             "FEYPQYYLAEPWAFSALSAYMFF"
             "LIIAGFPINFLTLYVTIEHKKLR"
             "TPLNYILLNLAVADLFMVFGGFT"
             "TTMYTSMHGYFVFGPTGCNIEGF"
             "FATLGGEIALWCLVVLAIERWMV"
             "VCKPVTNFRFGESHAIMGVMVTW"
             "TMALACALPPLFGWSRYIPEGLQ"
             "CSCGIDYYTRAPGINNESFVIYM"
             "FTCHFSIPLAVISFCYGRLVCTV"
             "KEAAAQQQESETTQRAEREVTRM"
             "VVIMVISFLVCWVPYASVAWYIF"
             "THQGSTFGPIFMTIPSFFAKSSA"
             "LYNPMIYICMNKQFRHCMITTLC"
             "CGKNPFEEEDGASATSSKTEASS"
             "VS",
         )
         self.assertEqual(alignments[6][1].id,
                          "gi|12583664|dbj|AB043817.1|")
         self.assertEqual(alignments[6][1].annotations["original_length"],
                          1344)
         self.assertEqual(len(alignments[7]), 2)
         self.assertEqual(alignments[7].get_alignment_length(), 111)
         self.assertEqual(
             alignments[7][0].seq,
             "VPFSNATGVVRSPFEYPQYYLAE"
             "PWQFSMLAAYMFLLIVLGFPINF"
             "LTLYVTVQHKKLRTPLNYILLNL"
             "AVADLFMVLGGFTSTLYTSLHGY"
             "FVFGPTGCNLEGFFATLGG",
         )
         self.assertEqual(alignments[7][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[7][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[7][1].seq,
             "VPFSNKTGVVRSPFEHPQYYLAE"
             "PWQFSMLAAYMFLLIVLGFPINF"
             "LTLYVTVQHKKLRTPLNYILLNL"
             "AVADLFMVFGGFTTTLYTSLHGY"
             "FVFGPTGCNLEGFFATLGG",
         )
         self.assertEqual(alignments[7][1].id,
                          "gi|283855845|gb|GQ290303.1|")
         self.assertEqual(alignments[7][1].annotations["original_length"],
                          4301)
         self.assertEqual(len(alignments[8]), 2)
         self.assertEqual(alignments[8].get_alignment_length(), 172)
         self.assertEqual(
             alignments[8][0].seq,
             "RYIPEGLQCSCGIDYYTLKPEVN"
             "NESFVIYMFVVHFTIPMIIIFFC"
             "YGQLVFTVKE-------------"
             "-----------------------"
             "AAAQQQESATTQKAEKEVTRMVI"
             "IMVIAFLICWVPYASVAFYIFTH"
             "QGSNFGPIFMTIPAFFAKSAAIY"
             "NPVIYIMMNKQ",
         )
         self.assertEqual(alignments[8][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[8][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[8][1].seq,
             "RYIPEGMQCSCGIDYYTLKPEVN"
             "NESFVIYMFVVHFTIPMIVIFFC"
             "YGQLVFTVKEVRSCVGHWGHAH*"
             "VNGAQLHSQSCHSLDT*PCVPA\\"
             "AAAQQQESATTQKAEKEVTRMVI"
             "IMVIAFLICWLPYAGVAFYIFTH"
             "QGSNFGPIFMTLPAFFAKSSSIY"
             "NPVIYIMMNKQ",
         )
         self.assertEqual(alignments[8][1].id,
                          "gi|283855845|gb|GQ290303.1|")
         self.assertEqual(alignments[8][1].annotations["original_length"],
                          4301)
         self.assertEqual(len(alignments[9]), 2)
         self.assertEqual(alignments[9].get_alignment_length(), 73)
         self.assertEqual(
             alignments[9][0].seq,
             "LGGEIALWSLVVLAIERYVVVCK"
             "PMSNFRFGENHAIMGVAFTWVMA"
             "LACAAPPLAGWSR--YIPEGLQC"
             "SCGI",
         )
         self.assertEqual(alignments[9][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[9][0].annotations["original_length"],
                          348)
         self.assertEqual(
             alignments[9][1].seq,
             "LAGEIALWSLVVLAIERYVVVCK"
             "PMSNFRFGENHAIMGLALTWVMA"
             "LACAAPPLVGWSR*WH*TEG-KC"
             "L*GL",
         )
         self.assertEqual(alignments[9][1].id,
                          "gi|283855845|gb|GQ290303.1|")
         self.assertEqual(alignments[9][1].annotations["original_length"],
                          4301)
         self.assertEqual(len(alignments[10]), 2)
         self.assertEqual(alignments[10].get_alignment_length(), 30)
         self.assertEqual(alignments[10][0].seq,
                          "IMMNKQFRNCMLTTICCGKNPLGDDEASAT")
         self.assertEqual(alignments[10][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[10][0].annotations["original_length"],
                          348)
         self.assertEqual(alignments[10][1].seq,
                          "MLLAFQFRNCMLTTLCCGKNPLGDDEASTT")
         self.assertEqual(alignments[10][1].id,
                          "gi|283855845|gb|GQ290303.1|")
         self.assertEqual(alignments[10][1].annotations["original_length"],
                          4301)
         self.assertEqual(len(alignments[11]), 2)
         self.assertEqual(alignments[11].get_alignment_length(), 31)
         self.assertEqual(alignments[11][0].seq,
                          "AQQQESATTQKAEKEVTRMVIIMVIAFLICW")
         self.assertEqual(alignments[11][0].id, "sp|P08100|OPSD_HUMAN")
         self.assertEqual(alignments[11][0].annotations["original_length"],
                          348)
         self.assertEqual(alignments[11][1].seq,
                          "SQQIRNATTMMMTMRVTSFSAFWVVADSCCW")
         self.assertEqual(alignments[11][1].id,
                          "gi|283855822|gb|GQ290312.1|")
         self.assertEqual(alignments[11][1].annotations["original_length"],
                          983)
Esempio n. 8
0
 def test_output007(self):
     """Check output007.m10 file."""
     fasta_file = "Fasta/output007.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 9)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 108)
         self.assertEqual(
             alignments[0][0].seq,
             "SGSNT-RRRAISRPVRLTAEED-"
             "--QEIRKRAAECGKTVSGFLRAA"
             "ALGKKVNSLTDDRVLKEVM----"
             "-RLGALQKKLFIDGKRVGDREYA"
             "EVLIAITEYHRALLSR",
         )
         self.assertEqual(alignments[0][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[0][1].seq,
             "AGSGAPRRRGSGLASRISEQSEA"
             "LLQEAAKHAAEFGRS------EV"
             "DTEHLLLALADSDVVKTILGQFK"
             "IKVDDLKRQIESEAKR-GDKPF-"
             "EGEIGVSPRVKDALSR",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|152973457|ref|YP_001338508.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          931)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 64)
         self.assertEqual(
             alignments[1][0].seq,
             "AAECGKTVSGFLRAAALGKKVNS"
             "LTDDRVLKEV-MRLGALQKKLFI"
             "DGKRVGDREYAEVLIAIT",
         )
         self.assertEqual(alignments[1][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[1][1].seq,
             "ASRQGCTVGG--KMDSVQDKASD"
             "KDKERVMKNINIMWNALSKNRLF"
             "DG----NKELKEFIMTLT",
         )
         self.assertEqual(alignments[1][1].id,
                          "gi|152973588|ref|YP_001338639.1|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          459)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 45)
         self.assertEqual(alignments[2][0].seq,
                          "EIRKRAAECGKTVSGFLRAAA-----LGKKVNSLTDDRVLKEVMR")
         self.assertEqual(alignments[2][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          107)
         self.assertEqual(alignments[2][1].seq,
                          "ELVKLIADMGISVRALLRKNVEPYEELGLEEDKFTDDQLIDFMLQ")
         self.assertEqual(alignments[2][1].id,
                          "gi|152973480|ref|YP_001338531.1|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          141)
         self.assertEqual(len(alignments[3]), 2)
         self.assertEqual(alignments[3].get_alignment_length(), 38)
         self.assertEqual(alignments[3][0].seq,
                          "MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIE")
         self.assertEqual(alignments[3][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[3][0].annotations["original_length"],
                          126)
         self.assertEqual(alignments[3][1].seq,
                          "IKKDLGVSFLKLKNREKTLIVDALKKKYPVAELLSVLQ")
         self.assertEqual(alignments[3][1].id,
                          "gi|152973462|ref|YP_001338513.1|")
         self.assertEqual(alignments[3][1].annotations["original_length"],
                          101)
         self.assertEqual(len(alignments[4]), 2)
         self.assertEqual(alignments[4].get_alignment_length(), 11)
         self.assertEqual(alignments[4][0].seq, "FFDLIIPNGGK")
         self.assertEqual(alignments[4][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[4][0].annotations["original_length"],
                          126)
         self.assertEqual(alignments[4][1].seq, "FFDLVIENPGK")
         self.assertEqual(alignments[4][1].id,
                          "gi|152973509|ref|YP_001338560.1|")
         self.assertEqual(alignments[4][1].annotations["original_length"],
                          448)
         self.assertEqual(len(alignments[5]), 2)
         self.assertEqual(alignments[5].get_alignment_length(), 40)
         self.assertEqual(alignments[5][0].seq,
                          "DKTLFIVYATDIYSPSE-FFSKIESDLKKKKSKGD-VFFD")
         self.assertEqual(alignments[5][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[5][0].annotations["original_length"],
                          126)
         self.assertEqual(alignments[5][1].seq,
                          "ESVVFILMAGFAMSVCYLFFSVLEKVINARKSKDESIYHD")
         self.assertEqual(alignments[5][1].id,
                          "gi|152973581|ref|YP_001338632.1|")
         self.assertEqual(alignments[5][1].annotations["original_length"],
                          84)
         self.assertEqual(len(alignments[6]), 2)
         self.assertEqual(alignments[6].get_alignment_length(), 30)
         self.assertEqual(alignments[6][0].seq,
                          "ASFFKKNFDKINVNLLSKATSFALKKGIPI")
         self.assertEqual(alignments[6][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[6][0].annotations["original_length"],
                          126)
         self.assertEqual(alignments[6][1].seq,
                          "ASFSKEEQDKVAVDKVAADVAWQERMNKPV")
         self.assertEqual(alignments[6][1].id,
                          "gi|152973536|ref|YP_001338587.1|")
         self.assertEqual(alignments[6][1].annotations["original_length"],
                          84)
         self.assertEqual(len(alignments[7]), 2)
         self.assertEqual(alignments[7].get_alignment_length(), 43)
         self.assertEqual(alignments[7][0].seq,
                          "SELHSKLPKSIDKIHEDIKKQLSC-SLIMKKIDVEMEDYSTYC")
         self.assertEqual(alignments[7][0].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[7][0].annotations["original_length"],
                          346)
         self.assertEqual(alignments[7][1].seq,
                          "SRINSDVARRIPGIHRDPKDRLSSLKQVEEALDMLISSHGEYC")
         self.assertEqual(alignments[7][1].id,
                          "gi|152973545|ref|YP_001338596.1|")
         self.assertEqual(alignments[7][1].annotations["original_length"],
                          242)
         self.assertEqual(len(alignments[8]), 2)
         self.assertEqual(alignments[8].get_alignment_length(), 64)
         self.assertEqual(
             alignments[8][0].seq,
             "ISGTYKGIDFLIKLMPSGGNTTI"
             "GRASGQNNTYFDEIALIIKENCL"
             "Y--SDTKNFEYTIPKFSD",
         )
         self.assertEqual(alignments[8][0].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[8][0].annotations["original_length"],
                          346)
         self.assertEqual(
             alignments[8][1].seq,
             "IDGVITAFD-LRTGMNISKDKVV"
             "AQIQGMDPVW---ISAAVPESIA"
             "YLLKDTSQFEISVPAYPD",
         )
         self.assertEqual(alignments[8][1].id,
                          "gi|152973505|ref|YP_001338556.1|")
         self.assertEqual(alignments[8][1].annotations["original_length"],
                          430)
Esempio n. 9
0
 def test_output001(self):
     """Check output001.m10 file."""
     fasta_file = "Fasta/output001.m10"
     with open(fasta_file) as handle:
         alignments = list(FastaIO.FastaM10Iterator(handle))
         self.assertEqual(len(alignments), 4)
         self.assertEqual(len(alignments[0]), 2)
         self.assertEqual(alignments[0].get_alignment_length(), 108)
         self.assertEqual(
             alignments[0][0].seq,
             "SGSNT-RRRAISRPVRLTAEED---QE"
             "IRKRAAECGKTVSGFLRAAALGKKVNS"
             "LTDDRVLKEVM-----RLGALQKKLFI"
             "DGKRVGDREYAEVLIAITEYHRALLSR",
         )
         self.assertEqual(alignments[0][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[0][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[0][1].seq,
             "AGSGAPRRRGSGLASRISEQSEALLQE"
             "AAKHAAEFGRS------EVDTEHLLLA"
             "LADSDVVKTILGQFKIKVDDLKRQIES"
             "EAKR-GDKPF-EGEIGVSPRVKDALSR",
         )
         self.assertEqual(alignments[0][1].id,
                          "gi|152973457|ref|YP_001338508.1|")
         self.assertEqual(alignments[0][1].annotations["original_length"],
                          931)
         self.assertEqual(len(alignments[1]), 2)
         self.assertEqual(alignments[1].get_alignment_length(), 64)
         self.assertEqual(
             alignments[1][0].seq,
             "AAECGKTVSGFLRAAALGKKVNSLTDD"
             "RVLKEV-MRLGALQKKLFIDGKRVGDR"
             "EYAEVLIAIT",
         )
         self.assertEqual(alignments[1][0].id,
                          "gi|10955263|ref|NP_052604.1|")
         self.assertEqual(alignments[1][0].annotations["original_length"],
                          107)
         self.assertEqual(
             alignments[1][1].seq,
             "ASRQGCTVGG--KMDSVQDKASDKDKE"
             "RVMKNINIMWNALSKNRLFDG----NK"
             "ELKEFIMTLT",
         )
         self.assertEqual(alignments[1][1].id,
                          "gi|152973588|ref|YP_001338639.1|")
         self.assertEqual(alignments[1][1].annotations["original_length"],
                          459)
         self.assertEqual(len(alignments[2]), 2)
         self.assertEqual(alignments[2].get_alignment_length(), 38)
         self.assertEqual(alignments[2][0].seq,
                          "MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIE")
         self.assertEqual(alignments[2][0].id,
                          "gi|10955264|ref|NP_052605.1|")
         self.assertEqual(alignments[2][0].annotations["original_length"],
                          126)
         self.assertEqual(alignments[2][1].seq,
                          "IKKDLGVSFLKLKNREKTLIVDALKKKYPVAELLSVLQ")
         self.assertEqual(alignments[2][1].id,
                          "gi|152973462|ref|YP_001338513.1|")
         self.assertEqual(alignments[2][1].annotations["original_length"],
                          101)
         self.assertEqual(len(alignments[3]), 2)
         self.assertEqual(alignments[3].get_alignment_length(), 43)
         self.assertEqual(alignments[3][0].seq,
                          "SELHSKLPKSIDKIHEDIKKQLSC-SLIMKKIDVEMEDYSTYC")
         self.assertEqual(alignments[3][0].id,
                          "gi|10955265|ref|NP_052606.1|")
         self.assertEqual(alignments[3][0].annotations["original_length"],
                          346)
         self.assertEqual(alignments[3][1].seq,
                          "SRINSDVARRIPGIHRDPKDRLSSLKQVEEALDMLISSHGEYC")
         self.assertEqual(alignments[3][1].id,
                          "gi|152973545|ref|YP_001338596.1|")
         self.assertEqual(alignments[3][1].annotations["original_length"],
                          242)