def make_start_fuzzy(self, location_object): ''' This function makes the start position of location objects fuzzy. ''' from Bio import SeqFeature if hasattr(location_object, 'parts'): if len(location_object.parts) == 1: new_start_pos = SeqFeature.BeforePosition(location_object.start) location_object = SeqFeature.FeatureLocation(new_start_pos, location_object.end) if len(location_object.parts) > 1: new_start_pos = SeqFeature.BeforePosition(location_object.parts[0].start) location_object.parts[0] = SeqFeature.FeatureLocation(new_start_pos, location_object.parts[0].end) return location_object
def _make_position(location_string, offset=0): """Turn a Swiss location position into a SeqFeature position object (PRIVATE). An offset of -1 is used with a start location to make it pythonic. """ if location_string == "?": return SeqFeature.UnknownPosition() # Hack so that feature from 0 to 0 becomes 0 to 0, not -1 to 0. try: return SeqFeature.ExactPosition(max(0, offset + int(location_string))) except ValueError: pass if location_string.startswith("<"): try: return SeqFeature.BeforePosition( max(0, offset + int(location_string[1:]))) except ValueError: pass elif location_string.startswith(">"): # e.g. ">13" try: return SeqFeature.AfterPosition( max(0, offset + int(location_string[1:]))) except ValueError: pass elif location_string.startswith("?"): # e.g. "?22" try: return SeqFeature.UncertainPosition( max(0, offset + int(location_string[1:]))) except ValueError: pass raise NotImplementedError("Cannot parse location '%s'" % location_string)
def _parse_position(element, offset=0): try: position = int(element.attrib["position"]) + offset except KeyError as err: position = None status = element.attrib.get("status", "") if status == "unknown": assert position is None return SeqFeature.UnknownPosition() elif not status: return SeqFeature.ExactPosition(position) elif status == "greater than": return SeqFeature.AfterPosition(position) elif status == "less than": return SeqFeature.BeforePosition(position) elif status == "uncertain": return SeqFeature.UncertainPosition(position) else: raise NotImplementedError("Position status %r" % status)
def get_newends(location, length, strand, end, distance, code, subject_start, feat_start, feat_finish, truncated): # Convert location if on reverse strand location = location if strand == 1 else abs(location - (2 * distance + 1)) # Generate the new end position # Correct by length of the match if at the finish end change = location + strand * length if end == "finish" else location # Multiply by 3 if AA change = change * 3 if code == 'A' else change # Calculate newend = subject_start + change # Apply new end to appropriate end if ((end == "start" and strand == 1) or (end == "finish" and strand == -1)): feat_start = SeqFeature.BeforePosition( newend) if truncated else SeqFeature.ExactPosition(newend) else: feat_finish = SeqFeature.AfterPosition( newend) if truncated else SeqFeature.ExactPosition(newend) return (feat_start, feat_finish)
def parse(self): """Parse the input.""" assert self.entry.tag == NS + 'entry' def append_to_annotations(key, value): if key not in self.ParsedSeqRecord.annotations: self.ParsedSeqRecord.annotations[key] = [] if value not in self.ParsedSeqRecord.annotations[key]: self.ParsedSeqRecord.annotations[key].append(value) def _parse_name(element): self.ParsedSeqRecord.name = element.text self.ParsedSeqRecord.dbxrefs.append(self.dbname + ':' + element.text) def _parse_accession(element): append_to_annotations( 'accessions', element.text) # to cope with SwissProt plain text parser self.ParsedSeqRecord.dbxrefs.append(self.dbname + ':' + element.text) def _parse_protein(element): """Parse protein names (PRIVATE).""" descr_set = False for protein_element in element.getchildren(): if protein_element.tag in [ NS + 'recommendedName', NS + 'alternativeName' ]: #recommendedName tag are parsed before #use protein fields for name and description for rec_name in protein_element.getchildren(): ann_key = '%s_%s' % (protein_element.tag.replace( NS, ''), rec_name.tag.replace(NS, '')) append_to_annotations(ann_key, rec_name.text) if (rec_name.tag == NS + 'fullName') and not descr_set: self.ParsedSeqRecord.description = rec_name.text descr_set = True elif protein_element.tag == NS + 'component': pass #not parsed elif protein_element.tag == NS + 'domain': pass #not parsed def _parse_gene(element): for genename_element in element.getchildren(): if 'type' in genename_element.attrib: ann_key = 'gene_%s_%s' % (genename_element.tag.replace( NS, ''), genename_element.attrib['type']) if genename_element.attrib['type'] == 'primary': self.ParsedSeqRecord.annotations[ ann_key] = genename_element.text else: append_to_annotations(ann_key, genename_element.text) def _parse_geneLocation(element): append_to_annotations('geneLocation', element.attrib['type']) def _parse_organism(element): organism_name = com_name = sci_name = '' for organism_element in element.getchildren(): if organism_element.tag == NS + 'name': if organism_element.text: if organism_element.attrib['type'] == 'scientific': sci_name = organism_element.text elif organism_element.attrib['type'] == 'common': com_name = organism_element.text else: #e.g. synonym append_to_annotations("organism_name", organism_element.text) elif organism_element.tag == NS + 'dbReference': self.ParsedSeqRecord.dbxrefs.append( organism_element.attrib['type'] + ':' + organism_element.attrib['id']) elif organism_element.tag == NS + 'lineage': for taxon_element in organism_element.getchildren(): if taxon_element.tag == NS + 'taxon': append_to_annotations('taxonomy', taxon_element.text) if sci_name and com_name: organism_name = '%s (%s)' % (sci_name, com_name) elif sci_name: organism_name = sci_name elif com_name: organism_name = com_name self.ParsedSeqRecord.annotations['organism'] = organism_name def _parse_organismHost(element): for organism_element in element.getchildren(): if organism_element.tag == NS + 'name': append_to_annotations("organism_host", organism_element.text) def _parse_keyword(element): append_to_annotations('keywords', element.text) def _parse_comment(element): """Parse comments (PRIVATE). Comment fields are very heterogeneus. each type has his own (frequently mutated) schema. To store all the contained data, more complex data structures are needed, such as annidated dictionaries. This is left to end user, by optionally setting: return_raw_comments=True the orginal XMLs is returned in the annotation fields. available comment types at december 2009: "allergen" "alternative products" "biotechnology" "biophysicochemical properties" "catalytic activity" "caution" "cofactor" "developmental stage" "disease" "domain" "disruption phenotype" "enzyme regulation" "function" "induction" "miscellaneous" "pathway" "pharmaceutical" "polymorphism" "PTM" "RNA editing" "similarity" "subcellular location" "sequence caution" "subunit" "tissue specificity" "toxic dose" "online information" "mass spectrometry" "interaction" """ simple_comments = [ "allergen", "biotechnology", "biophysicochemical properties", "catalytic activity", "caution", "cofactor", "developmental stage", "disease", "domain", "disruption phenotype", "enzyme regulation", "function", "induction", "miscellaneous", "pathway", "pharmaceutical", "polymorphism", "PTM", "RNA editing", #positions not parsed "similarity", "subunit", "tissue specificity", "toxic dose", ] if element.attrib['type'] in simple_comments: ann_key = 'comment_%s' % element.attrib['type'].replace( ' ', '') for text_element in element.getiterator(NS + 'text'): if text_element.text: append_to_annotations(ann_key, text_element.text) elif element.attrib['type'] == 'subcellular location': for subloc_element in element.getiterator( NS + 'subcellularLocation'): for el in subloc_element.getchildren(): if el.text: ann_key = 'comment_%s_%s' % ( element.attrib['type'].replace( ' ', ''), el.tag.replace(NS, '')) append_to_annotations(ann_key, el.text) elif element.attrib['type'] == 'interaction': for interact_element in element.getiterator(NS + 'interactant'): ann_key = 'comment_%s_intactId' % element.attrib['type'] append_to_annotations(ann_key, interact_element.attrib['intactId']) elif element.attrib['type'] == 'alternative products': for alt_element in element.getiterator(NS + 'isoform'): ann_key = 'comment_%s_isoform' % element.attrib[ 'type'].replace(' ', '') for id_element in alt_element.getiterator(NS + 'id'): append_to_annotations(ann_key, id_element.text) elif element.attrib['type'] == 'mass spectrometry': ann_key = 'comment_%s' % element.attrib['type'].replace( ' ', '') start = end = 0 for loc_element in element.getiterator(NS + 'location'): pos_els = loc_element.getiterator(NS + 'position') pos_els = list(pos_els) # this try should be avoided, maybe it is safer to skip postion parsing for mass spectrometry try: if pos_els: end = int(pos_els[0].attrib['position']) start = end - 1 else: start = int( loc_element.getiterator(NS + 'begin') [0].attrib['position']) - 1 end = int( loc_element.getiterator(NS + 'end') [0].attrib['position']) except: #undefined positions or erroneusly mapped pass mass = element.attrib['mass'] method = element.attrib[ 'mass'] #TODO - Check this, looks wrong! if start == end == 0: append_to_annotations(ann_key, 'undefined:%s|%s' % (mass, method)) else: append_to_annotations( ann_key, '%s..%s:%s|%s' % (start, end, mass, method)) elif element.attrib['type'] == 'sequence caution': pass #not parsed: few information, complex structure elif element.attrib['type'] == 'online information': for link_element in element.getiterator(NS + 'link'): ann_key = 'comment_%s' % element.attrib['type'].replace( ' ', '') for id_element in link_element.getiterator(NS + 'link'): append_to_annotations( ann_key, '%s@%s' % (element.attrib['name'], link_element.attrib['uri'])) #return raw XML comments if needed if self.return_raw_comments: ann_key = 'comment_%s_xml' % element.attrib['type'].replace( ' ', '') append_to_annotations(ann_key, ElementTree.tostring(element)) def _parse_dbReference(element): self.ParsedSeqRecord.dbxrefs.append(element.attrib['type'] + ':' + element.attrib['id']) #e.g. # <dbReference type="PDB" key="11" id="2GEZ"> # <property value="X-ray" type="method"/> # <property value="2.60 A" type="resolution"/> # <property value="A/C/E/G=1-192, B/D/F/H=193-325" type="chains"/> # </dbReference> if 'type' in element.attrib: if element.attrib['type'] == 'PDB': method = "" resolution = "" for ref_element in element.getchildren(): if ref_element.tag == NS + 'property': dat_type = ref_element.attrib['type'] if dat_type == 'method': method = ref_element.attrib['value'] if dat_type == 'resolution': resolution = ref_element.attrib['value'] if dat_type == 'chains': pairs = ref_element.attrib['value'].split(',') for elem in pairs: pair = elem.strip().split('=') if pair[1] != '-': #TODO - How best to store these, do SeqFeatures make sense? feature = SeqFeature.SeqFeature() feature.type = element.attrib['type'] feature.qualifiers[ 'name'] = element.attrib['id'] feature.qualifiers['method'] = method feature.qualifiers[ 'resolution'] = resolution feature.qualifiers['chains'] = pair[ 0].split('/') start = int(pair[1].split('-')[0]) - 1 end = int(pair[1].split('-')[1]) feature.location = SeqFeature.FeatureLocation( start, end) #self.ParsedSeqRecord.features.append(feature) for ref_element in element.getchildren(): if ref_element.tag == NS + 'property': pass # this data cannot be fitted in a seqrecord object with a simple list. however at least ensembl and EMBL parsing can be improved to add entries in dbxrefs def _parse_reference(element): reference = SeqFeature.Reference() authors = [] scopes = [] tissues = [] journal_name = '' pub_type = '' pub_date = '' for ref_element in element.getchildren(): if ref_element.tag == NS + 'citation': pub_type = ref_element.attrib['type'] if pub_type == 'submission': pub_type += ' to the ' + ref_element.attrib['db'] if 'name' in ref_element.attrib: journal_name = ref_element.attrib['name'] pub_date = ref_element.attrib.get('date', '') j_volume = ref_element.attrib.get('volume', '') j_first = ref_element.attrib.get('first', '') j_last = ref_element.attrib.get('last', '') for cit_element in ref_element.getchildren(): if cit_element.tag == NS + 'title': reference.title = cit_element.text elif cit_element.tag == NS + 'authorList': for person_element in cit_element.getchildren(): authors.append(person_element.attrib['name']) elif cit_element.tag == NS + 'dbReference': self.ParsedSeqRecord.dbxrefs.append( cit_element.attrib['type'] + ':' + cit_element.attrib['id']) if cit_element.attrib['type'] == 'PubMed': reference.pubmed_id = cit_element.attrib['id'] elif ref_element.attrib['type'] == 'MEDLINE': reference.medline_id = cit_element.attrib['id'] elif ref_element.tag == NS + 'scope': scopes.append(ref_element.text) elif ref_element.tag == NS + 'source': for source_element in ref_element.getchildren(): if source_element.tag == NS + 'tissue': tissues.append(source_element.text) if scopes: scopes_str = 'Scope: ' + ', '.join(scopes) else: scopes_str = '' if tissues: tissues_str = 'Tissue: ' + ', '.join(tissues) else: tissues_str = '' reference.location = [ ] #locations cannot be parsed since they are actually written in free text inside scopes so all the references are put in the annotation. reference.authors = ', '.join(authors) if journal_name: if pub_date and j_volume and j_first and j_last: reference.journal = REFERENCE_JOURNAL % dict( name=journal_name, volume=j_volume, first=j_first, last=j_last, pub_date=pub_date) else: reference.journal = journal_name reference.comment = ' | '.join( (pub_type, pub_date, scopes_str, tissues_str)) append_to_annotations('references', reference) def _parse_position(element, offset=0): try: position = int(element.attrib['position']) + offset except KeyError, err: position = None status = element.attrib.get('status', '') if status == 'unknown': assert position is None return SeqFeature.UnknownPosition() elif not status: return SeqFeature.ExactPosition(position) elif status == 'greater than': return SeqFeature.AfterPosition(position) elif status == 'less than': return SeqFeature.BeforePosition(position) elif status == 'uncertain': return SeqFeature.UncertainPosition(position) else: raise NotImplementedError("Position status %r" % status)
"""Test the Location code located in SeqFeature.py This checks to be sure fuzzy and non-fuzzy representations of locations are working properly. """ from Bio import SeqFeature # --- test fuzzy representations print("Testing fuzzy representations...") # check the positions alone exact_pos = SeqFeature.ExactPosition(5) within_pos_s = SeqFeature.WithinPosition(10, left=10, right=13) within_pos_e = SeqFeature.WithinPosition(13, left=10, right=13) between_pos_e = SeqFeature.BetweenPosition(24, left=20, right=24) before_pos = SeqFeature.BeforePosition(15) after_pos = SeqFeature.AfterPosition(40) print "Exact:", exact_pos print("Within (as start, %i): %s" % (int(within_pos_s), within_pos_s)) print("Within (as end, %i): %s" % (int(within_pos_e), within_pos_e)) print("Between (as end, %i): %s" % (int(between_pos_e), between_pos_e)) print "Before:", before_pos print "After:", after_pos # put these into Locations location1 = SeqFeature.FeatureLocation(exact_pos, within_pos_e) location2 = SeqFeature.FeatureLocation(before_pos, between_pos_e) location3 = SeqFeature.FeatureLocation(within_pos_s, after_pos) for location in [location1, location2, location3]:
from Bio import SeqFeature # 명확한 position start_pos=SeqFeature.ExactPosition(15) end_pos=SeqFeature.ExactPosition(30) location=SeqFeature.FeatureLocation(start_pos, end_pos) print(start_pos,end_pos,location) # 명확하지 않은 position start_pos2 = SeqFeature.AfterPosition(1) end_pos2 = SeqFeature.BeforePosition(8) # end_pos2 = SeqFeature.BetweenPosition(9, left=8, right=9) my_location = SeqFeature.FeatureLocation(start_pos2, end_pos2) print(start_pos2,end_pos2,my_location)