def quit(self):
#print "Stopping patch thread.."
geom = self.geometry()
uiState = {'window': str(self.saveState().toPercentEncoding()), 'geometry': [geom.x(), geom.y(), geom.width(), geom.height()]}
Manager.getManager().writeConfigFile(uiState, self.stateFile)
self.thread.stop(block=True)
def quit(self):
#print "Stopping patch thread.."
geom = self.geometry()
uiState = {'window': str(self.saveState().toPercentEncoding()), 'geometry': [geom.x(), geom.y(), geom.width(), geom.height()]}
Manager.getManager().writeConfigFile(uiState, self.stateFile)
self.thread.stop(block=True)
def quit(self):
geom = self.geometry()
uiState = {'window': str(self.saveState().toPercentEncoding()), 'geometry': [geom.x(), geom.y(), geom.width(), geom.height()]}
Manager.getManager().writeConfigFile(uiState, self.stateFile)
for cam in self.cameras:
cam.quit()
self.module.quit(fromUi=True)
def startClicked(self):
if self.ui.startBtn.isChecked():
if not self.thread.isRunning():
self.thread.start()
Manager.logMsg("Patch module started.")
self.ui.startBtn.setText('Stop')
else:
self.ui.startBtn.setEnabled(False)
self.thread.stop()
Manager.logMsg("Patch module stopped.")
def startClicked(self):
if self.ui.startBtn.isChecked():
if not self.thread.isRunning():
self.thread.start()
Manager.logMsg("Patch module started.")
self.ui.startBtn.setText('Stop')
else:
self.ui.startBtn.setEnabled(False)
self.thread.stop()
Manager.logMsg("Patch module stopped.")
def quit(self):
geom = self.geometry()
uiState = {
'window': str(self.saveState().toPercentEncoding()),
'geometry': [geom.x(), geom.y(), geom.width(), geom.height()],
'docks': self.cw.saveState()
}
Manager.getManager().writeConfigFile(uiState, self.stateFile)
for iface in self.interfaces.values():
iface.quit()
self.module.quit(fromUi=True)
def startAcquireClicked(self, mode):
"""User clicked the acquire video button.
"""
try:
self.cam.setParam('triggerMode', 'Normal', autoRestart=False)
self.setBinning(autoRestart=False)
self.setExposure(autoRestart=False)
self.updateRegion(autoRestart=False)
self.cam.start()
Manager.logMsg("Camera started aquisition.", importance=0)
except:
self.imagingCtrl.acquisitionStopped()
printExc("Error starting camera:")
def toggleAcquire(self):
if self.ui.acquireVideoBtn.isChecked():
try:
self.cam.setParam('triggerMode', 'Normal', autoRestart=False)
self.setBinning(autoRestart=False)
self.setExposure(autoRestart=False)
self.updateRegion(autoRestart=False)
self.cam.start()
Manager.logMsg("Camera started aquisition.", importance=0)
except:
self.ui.acquireVideoBtn.setChecked(False)
printExc("Error starting camera:")
else:
#print "ACQ untoggled, stop record"
self.toggleRecord(False)
self.cam.stop()
Manager.logMsg("Camera stopped acquisition.", importance=0)
def __init__(self, parent):
Qt.QWidget.__init__(self, parent)
self.manager = Manager.getManager()
self.ui = Ui_Form()
self.ui.setupUi(self)
self.current = None
self.widgets = {}
self.ui.fileInfoLayout = self.ui.formLayout_2
self.focusEventCatcher = FocusEventCatcher()
self.focusEventCatcher.sigLostFocus.connect(self.focusLost)
def __init__(self, parent):
QtGui.QWidget.__init__(self, parent)
self.manager = Manager.getManager()
self.ui = Ui_Form()
self.ui.setupUi(self)
self.current = None
self.widgets = {}
self.ui.fileInfoLayout = self.ui.formLayout_2
self.focusEventCatcher = FocusEventCatcher()
#QtCore.QObject.connect(self.focusEventCatcher, QtCore.SIGNAL('lostFocus'), self.focusLost)
self.focusEventCatcher.sigLostFocus.connect(self.focusLost)
def start(self):
try:
if self.selectedImager() is None:
raise Exception("No imaging device selected.")
prot = self.makeProtocol()
self.currentProtocol = prot
dh = Manager.getManager().getCurrentDir().getDir('ImageSequence', create=True, autoIncrement=True)
dhinfo = prot.copy()
del dhinfo['imager']
dh.setInfo(dhinfo)
prot['storageDir'] = dh
self.ui.startBtn.setText('Stop')
self.ui.zStackGroup.setEnabled(False)
self.ui.timelapseGroup.setEnabled(False)
self.ui.deviceCombo.setEnabled(False)
self.thread.start(prot)
except Exception:
self.threadStopped()
raise
def __init__(self, dm, config):
clampName = config['clampDev']
QtGui.QMainWindow.__init__(self)
self.setWindowTitle(clampName)
self.startTime = None
self.redrawCommand = 1
self.analysisItems = {
'inputResistance': u'Ω',
'accessResistance': u'Ω',
'capacitance': 'F',
'restingPotential': 'V',
'restingCurrent': 'A',
'fitError': ''
}
self.params = {
'mode': 'vc',
'rate': config.get('sampleRate', 100000),
'downsample': config.get('downsample', 3),
'cycleTime': .2,
'recordTime': 0.1,
'delayTime': 0.03,
'pulseTime': 0.05,
'icPulse': -30e-12,
'vcPulse': -10e-3,
'icHolding': 0,
'vcHolding': -65e-3,
'icHoldingEnabled': False,
'icPulseEnabled': True,
'vcHoldingEnabled': False,
'vcPulseEnabled': True,
'drawFit': True,
'average': 1,
}
self.paramLock = Mutex(QtCore.QMutex.Recursive)
self.manager = dm
self.clampName = clampName
self.thread = PatchThread(self)
self.cw = QtGui.QWidget()
self.setCentralWidget(self.cw)
self.ui = Ui_Form()
self.ui.setupUi(self.cw)
#self.logBtn = LogButton("Log")
#self.statusBar().addPermanentWidget(self.logBtn)
self.setStatusBar(StatusBar())
self.stateFile = os.path.join('modules', self.clampName + '_ui.cfg')
uiState = Manager.getManager().readConfigFile(self.stateFile)
if 'geometry' in uiState:
geom = QtCore.QRect(*uiState['geometry'])
self.setGeometry(geom)
if 'window' in uiState:
ws = QtCore.QByteArray.fromPercentEncoding(uiState['window'])
self.restoreState(ws)
self.ui.splitter_2.setSizes([self.width() / 4, self.width() * 3. / 4.])
self.ui.splitter.setStretchFactor(0, 30)
self.ui.splitter.setStretchFactor(1, 10)
self.plots = {}
for k in self.analysisItems:
p = PlotWidget()
p.setLabel('left', text=k, units=self.analysisItems[k])
self.ui.plotLayout.addWidget(p)
self.plots[k] = p
irp = self.plots['inputResistance']
irp.setLogMode(y=True, x=False)
irp.setYRange(6, 11)
self.ui.icPulseSpin.setOpts(dec=True,
step=1,
minStep=1e-12,
bounds=[None, None],
siPrefix=True,
suffix='A')
self.ui.vcPulseSpin.setOpts(dec=True,
step=1,
minStep=1e-3,
bounds=[None, None],
siPrefix=True,
suffix='V')
self.ui.icHoldSpin.setOpts(dec=True,
step=1,
minStep=1e-12,
bounds=[None, None],
siPrefix=True,
suffix='A')
self.ui.vcHoldSpin.setOpts(dec=True,
step=1,
minStep=1e-3,
bounds=[None, None],
siPrefix=True,
suffix='V')
self.ui.cycleTimeSpin.setOpts(dec=True,
step=1,
minStep=1e-6,
bounds=[0, None],
siPrefix=True,
suffix='s')
self.ui.pulseTimeSpin.setOpts(dec=True,
step=1,
minStep=1e-6,
bounds=[0, 1.],
siPrefix=True,
suffix='s')
self.ui.delayTimeSpin.setOpts(dec=True,
step=1,
minStep=1e-6,
bounds=[0, 1.],
siPrefix=True,
suffix='s')
self.stateGroup = WidgetGroup([
(self.ui.icPulseSpin, 'icPulse'),
(self.ui.vcPulseSpin, 'vcPulse'),
(self.ui.icHoldSpin, 'icHolding'),
(self.ui.vcHoldSpin, 'vcHolding'),
(self.ui.icPulseCheck, 'icPulseEnabled'),
(self.ui.vcPulseCheck, 'vcPulseEnabled'),
(self.ui.icHoldCheck, 'icHoldingEnabled'),
(self.ui.vcHoldCheck, 'vcHoldingEnabled'),
(self.ui.cycleTimeSpin, 'cycleTime'),
(self.ui.pulseTimeSpin, 'pulseTime'),
(self.ui.delayTimeSpin, 'delayTime'),
(self.ui.drawFitCheck, 'drawFit'),
(self.ui.averageSpin, 'average'),
])
self.stateGroup.setState(self.params)
self.ui.patchPlot.setLabel('left', text='Primary', units='A')
self.patchCurve = self.ui.patchPlot.plot(
pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
self.patchFitCurve = self.ui.patchPlot.plot(
pen=QtGui.QPen(QtGui.QColor(0, 100, 200)))
self.ui.commandPlot.setLabel('left', text='Command', units='V')
self.commandCurve = self.ui.commandPlot.plot(
pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
self.ui.startBtn.clicked.connect(self.startClicked)
self.ui.recordBtn.clicked.connect(self.recordClicked)
self.ui.bathModeBtn.clicked.connect(self.bathMode)
self.ui.patchModeBtn.clicked.connect(self.patchMode)
self.ui.cellModeBtn.clicked.connect(self.cellMode)
self.ui.monitorModeBtn.clicked.connect(self.monitorMode)
self.ui.resetBtn.clicked.connect(self.resetClicked)
self.thread.finished.connect(self.threadStopped)
self.thread.sigNewFrame.connect(self.handleNewFrame)
self.ui.vcModeRadio.toggled.connect(self.updateParams)
self.stateGroup.sigChanged.connect(self.updateParams)
## Configure analysis plots, curves, and data arrays
self.analysisCurves = {}
self.analysisData = {'time': []}
for n in self.analysisItems:
w = getattr(self.ui, n + 'Check')
w.clicked.connect(self.showPlots)
p = self.plots[n]
self.analysisCurves[n] = p.plot(
pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
for suf in ['', 'Std']:
self.analysisData[n + suf] = []
self.showPlots()
self.updateParams()
self.show()
self.bathMode()
def stopAcquireClicked(self):
self.cam.stop()
Manager.logMsg("Camera stopped acquisition.", importance=0)
def __init__(self, dm, clampName, modes):
QtGui.QMainWindow.__init__(self)
self.setWindowTitle(clampName)
self.startTime = None
self.redrawCommand = 1
self.analysisItems = {
'inputResistance': u'Ω',
'accessResistance': u'Ω',
'capacitance': 'F',
'restingPotential': 'V',
'restingCurrent': 'A',
'fitError': ''
}
self.params = modes.pop('default')
self.modes = modes
self.stylesheet = {}
self.paramLock = Mutex(QtCore.QMutex.Recursive)
self.manager = dm
self.clampName = clampName
self.thread = PatchThread(self)
self.cw = QtGui.QWidget()
self.setCentralWidget(self.cw)
self.ui = Ui_Form()
self.ui.setupUi(self.cw)
self.setStatusBar(StatusBar())
# Create one button for each configured mode
row = None
self.modeRows = []
rowLen = 0
def mkModeCallback(name):
return lambda: self.setMode(name)
for modeName, mode in modes.items():
if modeName == 'default':
continue
if row is None:
row = QtGui.QWidget()
layout = QtGui.QHBoxLayout()
row.setLayout(layout)
self.ui.modeLayout.addWidget(row)
self.modeRows.append(row)
btn = QtGui.QPushButton(modeName)
layout.addWidget(btn)
rowLen += btn.sizeHint().width()
if rowLen > 200:
row = None
rowLen = 0
btn.clicked.connect(mkModeCallback(modeName))
self.stateFile = os.path.join('modules', self.clampName + '_ui.cfg')
uiState = Manager.getManager().readConfigFile(self.stateFile)
if 'geometry' in uiState:
geom = QtCore.QRect(*uiState['geometry'])
self.setGeometry(geom)
if 'window' in uiState:
ws = QtCore.QByteArray.fromPercentEncoding(uiState['window'])
self.restoreState(ws)
self.ui.splitter_2.setSizes([self.width()/4, self.width()*3./4.])
self.plots = {}
for k in self.analysisItems:
p = PlotWidget()
p.setLabel('left', text=k, units=self.analysisItems[k])
self.ui.plotLayout.addWidget(p)
self.plots[k] = p
self.ui.icPulseSpin.setOpts(dec=True, step=1, minStep=1e-12, bounds=[None,None], siPrefix=True, suffix='A')
self.ui.vcPulseSpin.setOpts(dec=True, step=1, minStep=1e-3, bounds=[None,None], siPrefix=True, suffix='V')
self.ui.icHoldSpin.setOpts(dec=True, step=1, minStep=1e-12, bounds=[None,None], siPrefix=True, suffix='A')
self.ui.vcHoldSpin.setOpts(dec=True, step=1, minStep=1e-3, bounds=[None,None], siPrefix=True, suffix='V')
self.ui.cycleTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,None], siPrefix=True, suffix='s')
self.ui.pulseTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,1.], siPrefix=True, suffix='s')
self.ui.delayTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,1.], siPrefix=True, suffix='s')
self.stateGroup = WidgetGroup([
(self.ui.icPulseSpin, 'icPulse'),
(self.ui.vcPulseSpin, 'vcPulse'),
(self.ui.icHoldSpin, 'icHolding'),
(self.ui.vcHoldSpin, 'vcHolding'),
(self.ui.icPulseCheck, 'icPulseEnabled'),
(self.ui.vcPulseCheck, 'vcPulseEnabled'),
(self.ui.icHoldCheck, 'icHoldingEnabled'),
(self.ui.vcHoldCheck, 'vcHoldingEnabled'),
(self.ui.cycleTimeSpin, 'cycleTime'),
(self.ui.pulseTimeSpin, 'pulseTime'),
(self.ui.delayTimeSpin, 'delayTime'),
(self.ui.drawFitCheck, 'drawFit'),
(self.ui.averageSpin, 'average'),
])
self.stateGroup.setState(self.params)
self.ui.patchPlot.setLabel('left', text='Primary', units='A')
self.patchCurve = self.ui.patchPlot.plot(pen=QtGui.QPen(QtGui.QColor(200,200,200)))
self.patchFitCurve = self.ui.patchPlot.plot(pen=QtGui.QPen(QtGui.QColor(0,100,200)))
self.ui.commandPlot.setLabel('left', text='Command', units='V')
self.commandCurve = self.ui.commandPlot.plot(pen=QtGui.QPen(QtGui.QColor(200,200,200)))
self.ui.startBtn.clicked.connect(self.startClicked)
self.ui.recordBtn.clicked.connect(self.recordClicked)
self.ui.resetBtn.clicked.connect(self.resetClicked)
self.thread.finished.connect(self.threadStopped)
self.thread.sigNewFrame.connect(self.handleNewFrame)
self.ui.vcModeRadio.toggled.connect(self.updateParams)
self.stateGroup.sigChanged.connect(self.updateParams)
## Configure analysis plots, curves, and data arrays
self.analysisCurves = {}
self.analysisData = {'time': []}
for n in self.analysisItems:
w = getattr(self.ui, n+'Check')
w.clicked.connect(self.showPlots)
p = self.plots[n]
self.analysisCurves[n] = p.plot(pen=QtGui.QPen(QtGui.QColor(200,200,200)))
for suf in ['', 'Std']:
self.analysisData[n+suf] = []
self.showPlots()
self.updateParams()
self.show()
def __init__(self, dm, clampName):
QtGui.QMainWindow.__init__(self)
self.setWindowTitle(clampName)
self.startTime = None
self.redrawCommand = 1
self.analysisItems = {
'inputResistance': u'Ω',
'accessResistance': u'Ω',
'capacitance': 'F',
'restingPotential': 'V',
'restingCurrent': 'A',
'fitError': ''
}
self.params = {
'mode': 'vc',
'rate': 400000,
'downsample': 10,
'cycleTime': .2,
'recordTime': 0.1,
'delayTime': 0.03,
'pulseTime': 0.05,
'icPulse': -30e-12,
'vcPulse': -10e-3,
'icHolding': 0,
'vcHolding': -65e-3,
'icHoldingEnabled': False,
'icPulseEnabled': True,
'vcHoldingEnabled': False,
'vcPulseEnabled': True,
'drawFit': True,
'average': 1,
}
self.paramLock = Mutex(QtCore.QMutex.Recursive)
self.manager = dm
self.clampName = clampName
self.thread = PatchThread(self)
self.cw = QtGui.QWidget()
self.setCentralWidget(self.cw)
self.ui = Ui_Form()
self.ui.setupUi(self.cw)
#self.logBtn = LogButton("Log")
#self.statusBar().addPermanentWidget(self.logBtn)
self.setStatusBar(StatusBar())
self.stateFile = os.path.join('modules', self.clampName + '_ui.cfg')
uiState = Manager.getManager().readConfigFile(self.stateFile)
if 'geometry' in uiState:
geom = QtCore.QRect(*uiState['geometry'])
self.setGeometry(geom)
if 'window' in uiState:
ws = QtCore.QByteArray.fromPercentEncoding(uiState['window'])
self.restoreState(ws)
self.ui.splitter_2.setSizes([self.width()/4, self.width()*3./4.])
self.plots = {}
for k in self.analysisItems:
p = PlotWidget()
p.setLabel('left', text=k, units=self.analysisItems[k])
self.ui.plotLayout.addWidget(p)
self.plots[k] = p
#irp = self.plots['inputResistance']
#irp.setManualYScale()
#irp.setYLog(True)
#irp.setYRange(1e6, 1e11)
self.ui.icPulseSpin.setOpts(dec=True, step=1, minStep=1e-12, bounds=[None,None], siPrefix=True, suffix='A')
self.ui.vcPulseSpin.setOpts(dec=True, step=1, minStep=1e-3, bounds=[None,None], siPrefix=True, suffix='V')
self.ui.icHoldSpin.setOpts(dec=True, step=1, minStep=1e-12, bounds=[None,None], siPrefix=True, suffix='A')
self.ui.vcHoldSpin.setOpts(dec=True, step=1, minStep=1e-3, bounds=[None,None], siPrefix=True, suffix='V')
self.ui.cycleTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,None], siPrefix=True, suffix='s')
self.ui.pulseTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,1.], siPrefix=True, suffix='s')
self.ui.delayTimeSpin.setOpts(dec=True, step=1, minStep=1e-6, bounds=[0,1.], siPrefix=True, suffix='s')
self.stateGroup = WidgetGroup([
(self.ui.icPulseSpin, 'icPulse'),
(self.ui.vcPulseSpin, 'vcPulse'),
(self.ui.icHoldSpin, 'icHolding'),
(self.ui.vcHoldSpin, 'vcHolding'),
(self.ui.icPulseCheck, 'icPulseEnabled'),
(self.ui.vcPulseCheck, 'vcPulseEnabled'),
(self.ui.icHoldCheck, 'icHoldingEnabled'),
(self.ui.vcHoldCheck, 'vcHoldingEnabled'),
(self.ui.cycleTimeSpin, 'cycleTime'),
(self.ui.pulseTimeSpin, 'pulseTime'),
(self.ui.delayTimeSpin, 'delayTime'),
(self.ui.drawFitCheck, 'drawFit'),
(self.ui.averageSpin, 'average'),
])
self.stateGroup.setState(self.params)
self.ui.patchPlot.setLabel('left', text='Primary', units='A')
self.patchCurve = self.ui.patchPlot.plot(pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
self.patchFitCurve = self.ui.patchPlot.plot(pen=QtGui.QPen(QtGui.QColor(0, 100, 200)))
self.ui.commandPlot.setLabel('left', text='Command', units='V')
self.commandCurve = self.ui.commandPlot.plot(pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
self.ui.startBtn.clicked.connect(self.startClicked)
self.ui.recordBtn.clicked.connect(self.recordClicked)
self.ui.bathModeBtn.clicked.connect(self.bathMode)
self.ui.patchModeBtn.clicked.connect(self.patchMode)
self.ui.cellModeBtn.clicked.connect(self.cellMode)
self.ui.monitorModeBtn.clicked.connect(self.monitorMode)
self.ui.resetBtn.clicked.connect(self.resetClicked)
self.thread.finished.connect(self.threadStopped)
self.thread.sigNewFrame.connect(self.handleNewFrame)
self.ui.vcModeRadio.toggled.connect(self.updateParams)
self.stateGroup.sigChanged.connect(self.updateParams)
## Configure analysis plots, curves, and data arrays
self.analysisCurves = {}
self.analysisData = {'time': []}
for n in self.analysisItems:
w = getattr(self.ui, n+'Check')
w.clicked.connect(self.showPlots)
p = self.plots[n]
self.analysisCurves[n] = p.plot(pen=QtGui.QPen(QtGui.QColor(200, 200, 200)))
for suf in ['', 'Std']:
self.analysisData[n+suf] = []
self.showPlots()
self.updateParams()
self.show()
self.bathMode()
def __init__(self, module):
self.hasQuit = False
self.module = module # handle to the rest of the application
self.interfaces = OrderedDict() # owner: widget
self.docks = OrderedDict() # owner: dock
# Start building UI
Qt.QMainWindow.__init__(self)
self.setWindowTitle('Camera')
self.cw = dockarea.DockArea()
self.setCentralWidget(self.cw)
self.gv = pg.GraphicsView()
self.gvDock = dockarea.Dock(name="View", widget=self.gv, hideTitle=True, size=(600,600))
self.cw.addDock(self.gvDock)
# set up ViewBox
self.view = pg.ViewBox()
self.view.enableAutoRange(x=False, y=False)
self.view.setAspectLocked(True)
self.gv.setCentralItem(self.view)
# And a plot area for displaying depth-related information
self.depthPlot = pg.PlotWidget(labels={'left': ('Depth', 'm')})
self.depthPlot.setYRange(0, 1e-3)
self.depthPlot.setXRange(-1, 1)
self.depthPlot.hideAxis('bottom')
self.depthPlot.setMouseEnabled(x=False)
self.depthDock = pg.dockarea.Dock(name='Depth', widget=self.depthPlot)
self.cw.addDock(self.depthDock, 'right')
self.depthDock.hide()
# Add a group that will track to the center of the view
# self.trackedGroup = pg.GroupItem()
# self.view.addItem(self.trackedGroup)
# search for all devices that provide a cameraModuleInterface() method
man = Manager.getManager()
devices = [man.getDevice(dev) for dev in man.listDevices()]
ifaces = OrderedDict([(dev.name(), dev.cameraModuleInterface(self)) for dev in devices if hasattr(dev, 'cameraModuleInterface')])
# add each device's control panel in ots own dock
haveDevs = False
for dev, iface in ifaces.items():
if iface is not None:
haveDevs = True
self.addInterface(dev, iface)
# Add explanatory label if no devices were found
if not haveDevs:
label = Qt.QLabel("No imaging devices available")
label.setAlignment(Qt.Qt.AlignHCenter | Qt.Qt.AlignVCenter)
dock = dockarea.Dock(name="nocamera", widget=label, size=(100, 500), hideTitle=True)
self.cw.addDock(dock, 'left', self.gvDock)
# Add a dock with ROI buttons and plot
self.roiWidget = ROIPlotter(self)
self.roiDock = dockarea.Dock(name='ROI Plot', widget=self.roiWidget, size=(400, 10))
self.cw.addDock(self.roiDock, 'bottom', self.gvDock)
# Add timelapse / z stack / mosaic controls
self.sequencerWidget = ImageSequencer(self)
self.sequencerDock = dockarea.Dock(name='Image Sequencer', widget=self.sequencerWidget, size=(200, 10))
self.cw.addDock(self.sequencerDock, 'right', self.roiDock)
#grid = pg.GridItem()
#self.view.addItem(grid)
#Scale bar
self.scaleBar = pg.ScaleBar(100e-6, offset=(-20,-20))
self.scaleBar.setParentItem(self.view)
## Set up status bar labels
self.recLabel = Qt.QLabel()
self.rgnLabel = Qt.QLabel()
self.xyLabel = Qt.QLabel()
self.tLabel = Qt.QLabel()
self.vLabel = Qt.QLabel()
self.vLabel.setFixedWidth(50)
self.setStatusBar(StatusBar())
font = self.xyLabel.font()
font.setPointSize(8)
labels = [self.recLabel, self.xyLabel, self.rgnLabel, self.tLabel, self.vLabel]
for label in labels:
label.setFont(font)
self.statusBar().insertPermanentWidget(0, label)
# Load previous window state
self.stateFile = os.path.join('modules', self.module.name + '_ui.cfg')
uiState = module.manager.readConfigFile(self.stateFile)
if 'geometry' in uiState:
geom = Qt.QRect(*uiState['geometry'])
self.setGeometry(geom)
if 'window' in uiState:
ws = Qt.QByteArray.fromPercentEncoding(uiState['window'])
self.restoreState(ws)
if 'docks' in uiState:
self.cw.restoreState(uiState['docks'], missing='ignore')
# done with UI
self.show()
self.centerView()
self.gv.scene().sigMouseMoved.connect(self.updateMouse)
def __init__(self, camera, module):
CameraModuleInterface.__init__(self, camera, module)
self.module = module
self.view = module.getView()
self.hasQuit = False
self.boundaryItems = {}
## setup UI
self.ui = CameraInterfaceTemplate()
self.widget = dockarea.DockArea()
w = Qt.QWidget()
self.ui.setupUi(w)
# takes care of displaying image data,
# contrast & background subtraction user interfaces
self.imagingCtrl = ImagingCtrl()
self.frameDisplay = self.imagingCtrl.frameDisplay
## Move control panels into docks
recDock = dockarea.Dock(name="Recording",
widget=self.imagingCtrl,
size=(100, 10),
autoOrientation=False)
devDock = dockarea.Dock(name="Device Control",
widget=self.ui.devCtrlWidget,
size=(100, 10),
autoOrientation=False)
dispDock = dockarea.Dock(name="Display Control",
widget=self.frameDisplay.contrastWidget(),
size=(100, 600),
autoOrientation=False)
bgDock = dockarea.Dock(name="Background Subtraction",
widget=self.frameDisplay.backgroundWidget(),
size=(100, 10),
autoOrientation=False)
self.widget.addDock(recDock)
self.widget.addDock(devDock, 'bottom', recDock)
self.widget.addDock(dispDock, 'bottom', devDock)
self.widget.addDock(bgDock, 'bottom', dispDock)
## Camera state variables
self.cam = camera
self.roi = None
self.exposure = 0.001
self.binning = 1
self.region = None
## set up item groups
self.cameraItemGroup = pg.ItemGroup(
) ## translated with scope, scaled with camera objective
self.imageItemGroup = pg.ItemGroup(
) ## translated and scaled as each frame arrives
self.view.addItem(self.imageItemGroup)
self.view.addItem(self.cameraItemGroup)
self.cameraItemGroup.setZValue(0)
self.imageItemGroup.setZValue(-2)
## video image item
self.imageItem = self.frameDisplay.imageItem()
self.view.addItem(self.imageItem)
self.imageItem.setParentItem(self.imageItemGroup)
self.imageItem.setZValue(-10)
## open camera, determine bit depth and sensor area
self.openCamera()
## Initialize values
self.lastCameraPosition = Point(self.camSize[0] * 0.5,
self.camSize[1] * 0.5)
self.lastCameraScale = Point(1.0, 1.0)
self.scopeCenter = [self.camSize[0] * 0.5, self.camSize[1] * 0.5]
self.cameraScale = [1, 1]
## Camera region-of-interest control
self.roi = CamROI(self.camSize, parent=self.cameraItemGroup)
self.roi.sigRegionChangeFinished.connect(self.regionWidgetChanged)
self.roi.setZValue(-1)
self.setRegion()
## Set up microscope objective borders
self.borders = CameraItemGroup(self.cam)
self.module.addItem(self.borders)
self.borders.setZValue(-1)
self.cam.sigGlobalTransformChanged.connect(self.globalTransformChanged)
self.globalTransformChanged()
# initially set binning and exposure from camera state
self.exposure = self.cam.getParam('exposure')
self.binning = self.cam.getParam('binning')[0]
## Initialize values/connections in Camera Dock
self.setUiBinning(self.binning)
self.ui.spinExposure.setValue(self.exposure)
self.ui.spinExposure.setOpts(dec=True,
step=1,
minStep=100e-6,
siPrefix=True,
suffix='s',
bounds=[0, 10])
#Signals from self.ui.btnSnap and self.ui.recordStackBtn are caught by the RecordThread
self.ui.btnFullFrame.clicked.connect(lambda: self.setRegion())
self.binningComboProxy = SignalProxy(
self.ui.binningCombo.currentIndexChanged,
slot=self.binningComboChanged)
self.ui.spinExposure.valueChanged.connect(
self.setExposure
) ## note that this signal (from acq4.util.SpinBox) is delayed.
## Signals from Camera device
self.cam.sigNewFrame.connect(self.newFrame)
self.cam.sigCameraStopped.connect(self.cameraStopped)
self.cam.sigCameraStarted.connect(self.cameraStarted)
self.cam.sigShowMessage.connect(self.showMessage)
self.cam.sigParamsChanged.connect(self.cameraParamsChanged)
self.frameDisplay.imageUpdated.connect(self.imageUpdated)
self.imagingCtrl.sigStartVideoClicked.connect(self.startAcquireClicked)
self.imagingCtrl.sigStopVideoClicked.connect(self.stopAcquireClicked)
self.imagingCtrl.ui.acquireFrameBtn.setEnabled(False)
# Hotkey signals
for action, key in self.cam.camConfig.get('hotkeys', {}).items():
if action not in ['snap', 'start']:
raise ValueError("Unknown hotkey action %r" % action)
dev = Manager.getManager().getDevice(key['device'])
dev.addKeyCallback(key['key'], self.hotkeyPressed, (action, ))
def __init__(self, module):
self.hasQuit = False
self.module = module ## handle to the rest of the application
## ROI state variables
self.lastPlotTime = None
self.ROIs = []
self.plotCurves = []
## Start building UI
QtGui.QMainWindow.__init__(self)
self.cw = dockarea.DockArea()
self.setCentralWidget(self.cw)
self.gv = pg.GraphicsView()
self.gvDock = dockarea.Dock(name="View", widget=self.gv, hideTitle=True, size=(600,600))
self.cw.addDock(self.gvDock)
## Load previous window state
self.stateFile = os.path.join('modules', self.module.name + '_ui.cfg')
uiState = module.manager.readConfigFile(self.stateFile)
if 'geometry' in uiState:
geom = QtCore.QRect(*uiState['geometry'])
self.setGeometry(geom)
if 'window' in uiState:
ws = QtCore.QByteArray.fromPercentEncoding(uiState['window'])
self.restoreState(ws)
self.show()
## set up ViewBox
self.view = pg.ViewBox()
self.view.setAspectLocked(True)
self.gv.setCentralItem(self.view)
## build camera control panels
man = Manager.getManager()
camNames = man.listInterfaces('camera')
self.cameras = []
self.cameraDocks = []
if len(camNames) == 0:
label = QtGui.QLabel("No cameras available")
label.setAlignment(QtCore.Qt.AlignHCenter | QtCore.Qt.AlignVCenter)
dock = dockarea.Dock(name="nocamera", widget=label, size=(100, 500), hideTitle=True)
self.cw.addDock(dock, 'left', self.gvDock)
else:
for name in camNames:
camera = man.getInterface('camera', name)
iface = camera.cameraModuleInterface(self)
self.cameras.append(iface)
dock = dockarea.Dock(name=name, widget=iface.controlWidget(), size=(10, 500), hideTitle=(len(camNames)==1))
if len(self.cameraDocks) == 0:
self.cw.addDock(dock, 'left', self.gvDock)
else:
self.cw.addDock(dock, 'below', self.cameraDocks[0])
self.cameraDocks.append(dock)
iface.sigNewFrame.connect(self.newFrame)
## ROI plot ctrls
self.roiWidget = QtGui.QWidget()
self.roiLayout = QtGui.QGridLayout()
self.roiLayout.setSpacing(0)
self.roiLayout.setContentsMargins(0,0,0,0)
self.roiWidget.setLayout(self.roiLayout)
rectPath = QtGui.QPainterPath()
rectPath.addRect(0, 0, 1, 1)
self.rectBtn = pg.PathButton(path=rectPath)
ellPath = QtGui.QPainterPath()
ellPath.addEllipse(0, 0, 1, 1)
self.ellipseBtn = pg.PathButton(path=ellPath)
polyPath = QtGui.QPainterPath()
polyPath.moveTo(0,0)
polyPath.lineTo(2,3)
polyPath.lineTo(3,1)
polyPath.lineTo(5,0)
polyPath.lineTo(2, -2)
polyPath.lineTo(0,0)
self.polygonBtn = pg.PathButton(path=polyPath)
polyPath = QtGui.QPainterPath()
polyPath.moveTo(0,0)
polyPath.lineTo(2,3)
polyPath.lineTo(3,1)
polyPath.lineTo(5,0)
self.polylineBtn = pg.PathButton(path=polyPath)
self.roiLayout.addWidget(self.rectBtn, 0, 0)
self.roiLayout.addWidget(self.ellipseBtn, 0, 1)
self.roiLayout.addWidget(self.polygonBtn, 1, 0)
self.roiLayout.addWidget(self.polylineBtn, 1, 1)
self.roiTimeSpin = pg.SpinBox(value=5.0, suffix='s', siPrefix=True, dec=True, step=0.5, bounds=(0,None))
self.roiLayout.addWidget(self.roiTimeSpin, 2, 0, 1, 2)
self.roiPlotCheck = QtGui.QCheckBox('Plot')
self.roiLayout.addWidget(self.roiPlotCheck, 3, 0, 1, 2)
self.roiPlot = pg.PlotWidget()
self.roiLayout.addWidget(self.roiPlot, 0, 2, self.roiLayout.rowCount(), 1)
self.roiDock = dockarea.Dock(name='ROI Plot', widget=self.roiWidget, size=(600, 10))
self.cw.addDock(self.roiDock, 'bottom', self.gvDock)
#grid = pg.GridItem()
#self.view.addItem(grid)
##Scale bar
self.scaleBar = pg.ScaleBar(100e-6, offset=(-20,-20))
self.scaleBar.setParentItem(self.view)
### Set up status bar labels
self.recLabel = QtGui.QLabel()
#self.fpsLabel = pg.ValueLabel(averageTime=2.0, formatStr='{avgValue:.1f} fps')
#self.displayFpsLabel = pg.ValueLabel(averageTime=2.0, formatStr='(displaying {avgValue:.1f} fps')
#self.displayPercentLabel = pg.ValueLabel(averageTime=4.0, formatStr='{avgValue:.1f}%)')
self.rgnLabel = QtGui.QLabel()
self.xyLabel = QtGui.QLabel()
self.tLabel = QtGui.QLabel()
self.vLabel = QtGui.QLabel()
#self.fpsLabel.setFixedWidth(50)
#self.displayFpsLabel.setFixedWidth(100)
#self.displayFpsLabel.setFixedWidth(100)
self.vLabel.setFixedWidth(50)
#self.logBtn = LogButton('Log')
self.setStatusBar(StatusBar())
font = self.xyLabel.font()
font.setPointSize(8)
labels = [self.recLabel, self.xyLabel, self.rgnLabel, self.tLabel, self.vLabel]
for label in labels:
label.setFont(font)
self.statusBar().insertPermanentWidget(0, label)
## done with UI
self.show()
#self.autoRange()
self.centerView()
self.gv.scene().sigMouseMoved.connect(self.updateMouse)
## Connect ROI dock
self.rectBtn.clicked.connect(self.addROI)
#self.ui.btnClearROIs.clicked.connect(self.clearROIs)
#self.roiPlotCheck.stateChanged.connect(self.enableROIsChanged)
self.roiTimeSpin.valueChanged.connect(self.setROITime)
