def test_per_base(self):
pos = [1, 12, 15, 27]
# boolean array, all true
b = [True, True, True, True]
# N.B., final bin includes right edge
expected_nnz = [1, 2, 1]
expected_windows = [[1, 10], [11, 20], [21, 27]]
expected_counts = [1, 2, 1]
expected_densities = [1 / 10, 2 / 10, 1 / 7]
expected_n_bases = [10, 10, 7]
nnz, windows, counts = allel.windowed_statistic(
pos, b, statistic=np.count_nonzero, size=10, start=1)
densities, n_bases = allel.per_base(nnz, windows)
aeq(expected_nnz, nnz)
aeq(expected_windows, windows)
aeq(expected_counts, counts)
aeq(expected_densities, densities)
aeq(expected_n_bases, n_bases)
# boolean array, not all true
b = [False, True, False, True]
expected_densities = [0 / 10, 1 / 10, 1 / 7]
expected_n_bases = [10, 10, 7]
nnz, windows, counts = allel.windowed_statistic(
pos, b, statistic=np.count_nonzero, size=10, start=1)
densities, n_bases = allel.per_base(nnz, windows)
aeq(expected_densities, densities)
aeq(expected_n_bases, n_bases)
# 2D, 4 variants, 2 samples
b = [[True, False], [True, True], [True, False], [True, True]]
expected_densities = [[1 / 10, 0 / 10], [2 / 10, 1 / 10],
[1 / 7, 1 / 7]]
expected_n_bases = [10, 10, 7]
nnz, windows, counts = allel.windowed_statistic(
pos, b, statistic=lambda x: np.sum(x, axis=0), size=10, start=1)
densities, n_bases = allel.per_base(nnz, windows)
aeq(expected_densities, densities)
aeq(expected_n_bases, n_bases)
# include is_accessible array option
is_accessible = np.array([
0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 0, 0, 1, 1,
1, 1, 1, 1, 1, 1, 1, 1
],
dtype=bool)
b = [False, True, False, True]
expected_densities = [-1, 1 / 6, 1 / 7]
expected_n_bases = [0, 6, 7]
nnz, windows, counts = allel.windowed_statistic(
pos, b, statistic=np.count_nonzero, size=10, start=1)
densities, n_bases = allel.per_base(nnz,
windows,
is_accessible=is_accessible,
fill=-1)
aeq(expected_densities, densities)
aeq(expected_n_bases, n_bases)
def mutrate(ps, polymorphic_loci_outgroup, chrom):
'''
Input:
- ps,
- polymorphic_loci_outgroup,
- chrom
Output:
- ingroup_index : np array with shape (x, ) x being the number of ingroup individuals.
The ingroup is considered any non-african from HGDP.
'''
#Counts of polymorphic sites in windows of 1Kb
snp_count, windows, _ = allel.windowed_statistic(
pos=ps,
values=polymorphic_loci_outgroup,
statistic=np.sum,
windows=get_windowed(chrom),
fill=0)
#np array precursor of the final mutation rate. The columns are:
# 1. Chromosome
# 2. Start coordinate for the window (0-based, included)
# 3. Number of polymorphic sites
# 4. Percentage of callable bases in that window
mut_rate = pd.DataFrame({
"chrom": [chrom] * windows.shape[0],
"start": (windows[:, 0] - 1).astype("int32"),
"segregating":
snp_count,
"call":
np.loadtxt(
"/home/moicoll/GenerationInterval/people/moi/tmp/weigths/chr{}_weigths.txt"
.format(chrom),
usecols=[2])
})
#average genomic mutation rate in the inputed chromosome
genomic_mut_rate = np.sum(mut_rate["segregating"]) / np.sum(
mut_rate["call"])
#average genomic mutation rate in the inputed chromosome over 1Mb. However, a row per 1Kb window is given.
mut_rate["mut_rate"] = (mut_rate.assign(
start_big_window=(mut_rate["start"] / 1000000).astype(int)).groupby(
"start_big_window", group_keys=False
).apply(lambda x: ((x["start"] + 1) / (x["start"] + 1)) * np.sum(x[
"segregating"]) / np.sum(x["call"]) / genomic_mut_rate).fillna(0))
#save dataframe, droping columns "segregating" and "call"
mut_rate.drop(["segregating", "call"], axis=1).to_csv(
"/home/moicoll/GenerationInterval/people/moi/tmp/mutrate/chr{}.tmp".
format(chrom),
header=False,
index=False,
sep='\t')
meta_data_samples['callset_index'] = samples_callset_index
def het_counting(gt):
return gt.count_het()
gt_zarr = callset["{}/calldata/GT".format(chrom)]
pos = callset["{}/variants/POS".format(chrom)]
gt = allel.GenotypeDaskArray(gt_zarr)
df_list = []
for i, row in meta_data_samples.iterrows():
df = pd.DataFrame()
individual = (gt.take([row.callset_index], axis=1))
nnz, windows, counts = allel.windowed_statistic(pos,
individual,
statistic=het_counting,
size=window_size)
df["het"] = nnz
if i % 10 == 0:
print(i)
window_numbering = []
df.insert(0, column="chr", value=chrom)
window_numbering.extend(range(len(nnz)))
df.insert(1, column="window", value=window_numbering)
df.insert(2, column="PGDP_ID", value=row.PGDP_ID)
df_list.append(df)
chr_df = pd.concat(df_list, axis=0)
chr_df.to_csv("../steps/het_counts_windows_{}.txt".format(chrom),
sep=" ",
index=False)
print("Finished with {}".format(chrom))
# Use the middle of the window as the index
window_middle = np.sum(eqa, axis=1)/2
vref_dxy_by_window[chrom] = pd.DataFrame(index=window_middle.astype(int), columns=list(subpops.keys()) + list(species.keys()))
xpop_dxy_by_window[chrom] = pd.DataFrame(index=window_middle.astype(int))
# Calculate distance from the reference in each sub-population
for pop in subpops.keys():
print('processing', pop)
# Faster if we drop non variant loci first, and load into mem
loc = ac[pop].is_variant().compute()
pop_ac = ac[pop].compress(loc, axis=0).compute()
pop_pos = pos.compress(loc, axis=0)
print('computing divergence...', loc.sum())
vals, windows, counts = allel.windowed_statistic(
pop_pos, pop_ac.to_frequencies(), compute_divergence, windows=eqa)
vref_dxy_by_window[chrom][pop] = vals / window_size
# Calculate distance from the reference in each species
for pop in species.keys():
print('processing', pop)
# Faster if we drop non variant loci first, and load into mem
loc = ac_species[pop].is_variant().compute()
pop_ac = ac_species[pop].compress(loc, axis=0).compute()
pop_pos = pos.compress(loc, axis=0)
print('computing divergence...', loc.sum())
vals, windows, counts = allel.windowed_statistic(
pop_pos, pop_ac.to_frequencies(), compute_divergence, windows=eqa)
vref_dxy_by_window[chrom][pop] = vals / window_size
hap_div = allel.haplotype_diversity(region_complete)
# calculate nucleotide diversity specifically on nonmissing region
ac = region_complete.count_alleles()
diffs = allel.mean_pairwise_difference(ac, fill=0)
pi = np.sum(diffs) / 200
return [nh, hap_div, pi, freqs]
chromosomes = allel.read_vcf(vcf_path, fields=['CHROM'])
chromosomes_list = np.unique(chromosomes['variants/CHROM'])
for chrom in chromosomes_list:
print(chrom)
# read in that chromosome data only
callset = allel.read_vcf(vcf_path, region=chrom, fields='*')
gt = allel.GenotypeArray(callset["calldata/GT"])
# remove any het calls, convert to haploid
gt.mask = gt.is_het()
gt_hom_only = gt.fill_masked(value=-1)
gt_hap_array = gt_hom_only.haploidify_samples()
# remove individuals with missing data and calculate stats
n_list, w, n = allel.windowed_statistic(pos=callset["variants/POS"],
values=gt_hap_array,
statistic=removeMissingStats,
size=200,
step=50,
start=1)
df = pd.DataFrame(list(zip(n_list, w, n)),
columns=["n_list", "windows_n", "n_var_n"])
file_name = os.getcwd() + "/" + prefix + chrom + "_hap_div.csv"
df.to_csv(file_name, header=True)
