def test_reaction_cofactor_swap_target(self, model):
cofactor_id_swaps = [("nad_c", "nadh_c"), ("nadp_c", "nadph_c")]
swap_pairs = ([
model.metabolites.get_by_id(m) for m in cofactor_id_swaps[0]
], [model.metabolites.get_by_id(m) for m in cofactor_id_swaps[1]])
swap_target = ReactionCofactorSwapTarget("GAPD", swap_pairs)
with model:
swap_target.apply(model)
assert model.metabolites.nad_c not in model.reactions.GAPD.metabolites
assert model.metabolites.nadh_c not in model.reactions.GAPD.metabolites
assert model.metabolites.nadp_c in model.reactions.GAPD.metabolites
assert model.metabolites.nadph_c in model.reactions.GAPD.metabolites
assert model.metabolites.nadp_c not in model.reactions.GAPD.metabolites
assert model.metabolites.nadph_c not in model.reactions.GAPD.metabolites
assert model.metabolites.nad_c in model.reactions.GAPD.metabolites
assert model.metabolites.nadh_c in model.reactions.GAPD.metabolites
swap_target = ReactionCofactorSwapTarget("GND", swap_pairs)
with model:
swap_target.apply(model)
assert model.metabolites.nad_c in model.reactions.GND.metabolites
assert model.metabolites.nadh_c in model.reactions.GND.metabolites
assert model.metabolites.nadp_c not in model.reactions.GND.metabolites
assert model.metabolites.nadph_c not in model.reactions.GND.metabolites
assert model.metabolites.nadp_c in model.reactions.GND.metabolites
assert model.metabolites.nadph_c in model.reactions.GND.metabolites
assert model.metabolites.nad_c not in model.reactions.GND.metabolites
assert model.metabolites.nadh_c not in model.reactions.GND.metabolites
def test_invalid_reaction_cofactor_swap_target(self, model):
cofactor_id_swaps = [("nad_c", "nadh_c"), ("nadp_c", "nadph_c")]
swap_pairs = ([model.metabolites.get_by_id(m) for m in cofactor_id_swaps[0]],
[model.metabolites.get_by_id(m) for m in cofactor_id_swaps[1]])
swap_target = ReactionCofactorSwapTarget("GAPD_124", swap_pairs)
with pytest.raises(KeyError):
swap_target.apply(model)
swap_target = ReactionCofactorSwapTarget("ACKr", swap_pairs)
with pytest.raises(ValueError):
swap_target.apply(model)