def __init__(self,
numReps,
repDuration,
imagesPerRep,
sliceHeight=0,
metadata='',
savePath=''):
## Number of images to be collected per camera per rep.
self.imagesPerRep = imagesPerRep
## List of cameras. Assume our cameras are all active cameras.
self.cameras = []
for cam in depot.getHandlersOfType(depot.CAMERA):
if cam.getIsEnabled():
self.cameras.append(cam)
## List of light sources. Assume our lights are all active lights.
self.lights = []
for light in depot.getHandlersOfType(depot.LIGHT_TOGGLE):
if light.getIsEnabled():
self.lights.append(light)
super().__init__(numReps,
repDuration,
None,
0,
0,
sliceHeight,
self.cameras,
self.lights, {},
metadata=metadata,
savePath=savePath)
def __init__(self, parent):
super().__init__(parent)
self.Sizer = wx.BoxSizer(wx.VERTICAL)
self.Sizer.Add(PanelLabel(self, label="Lights"))
sz = wx.BoxSizer(wx.HORIZONTAL)
self.Sizer.Add(sz)
lightToggles = sorted(depot.getHandlersOfType(depot.LIGHT_TOGGLE),
key=lambda l: l.wavelength)
lightPowers = depot.getHandlersOfType(depot.LIGHT_POWER)
lightFilters = list(
filter(lambda f: f.lights,
depot.getHandlersOfType(depot.LIGHT_FILTER)))
self.panels = {}
for light in lightToggles:
power = next(
filter(lambda p: p.groupName == light.groupName, lightPowers),
None)
filters = list(
filter(lambda f: light.name in f.lights, lightFilters))
panel = LightPanel(self, light, power, filters)
sz.Add(panel, flag=wx.EXPAND)
self.panels[light] = panel
sz.AddSpacer(4)
self.Fit()
def __init__(self):
## Maps axis to the handlers for that axis, sorted by their range of
# motion.
self.axisToHandlers = depot.getSortedStageMovers()
## XXX: We have a single index for all axis, even though each
## axis may have a different number of stages. While we don't
## refactor this assumption, we just make copies of the movers
## with the most precise movement (issues #413 and #415)
self.n_stages = max([len(s) for s in self.axisToHandlers.values()])
for axis, stages in self.axisToHandlers.items():
stages.extend([stages[-1]] * (self.n_stages - len(stages)))
## Indicates which stage handler is currently under control.
self.curHandlerIndex = 0
## Maps Site unique IDs to Site instances.
self.idToSite = {}
## Maps handler names to events indicating if those handlers
# have stopped moving.
self.nameToStoppedEvent = {}
events.subscribe("stage mover", self.onMotion)
events.subscribe("stage stopped", self.onStop)
## Device-speficic primitives to draw on the macrostage.
self.primitives = set()
for h in depot.getHandlersOfType(depot.STAGE_POSITIONER):
ps = h.getPrimitives()
if ps:
self.primitives.update(ps)
self.primitives.discard(None)
def __init__(self, parent):
wx.Frame.__init__(self, parent, title = "Camera views",
style=wx.FRAME_NO_TASKBAR | wx.CAPTION)
self.numCameras = len(depot.getHandlersOfType(depot.CAMERA))
self.panel = wx.Panel(self)
# Make a 2xN grid of camera canvases, with menus above for selecting
# which camera to use in that location.
self.sizer = wx.FlexGridSizer(2, 5, 5)
## List of ViewPanels we contain.
self.views = []
for i in range(self.numCameras):
view = viewPanel.ViewPanel(self.panel)
self.views.append(view)
self.SetPosition((675, 280))
events.subscribe(events.CAMERA_ENABLE, self.onCameraEnableEvent)
events.subscribe("image pixel info", self.onImagePixelInfo)
cockpit.gui.keyboard.setKeyboardHandlers(self)
self.Bind(wx.EVT_CLOSE, self.onClose)
self.resetGrid()
self.SetDropTarget(cockpit.gui.viewFileDropTarget.ViewFileDropTarget(self))
def __init__(self, parent, configKey):
super().__init__(parent=parent)
self.configKey = configKey + self._CONFIG_KEY_SUFFIX
self.allLights = depot.getHandlersOfType(depot.LIGHT_TOGGLE)
self.settings = self.loadSettings()
sizer = wx.BoxSizer(wx.VERTICAL)
rowSizer = wx.BoxSizer(wx.HORIZONTAL)
text = wx.StaticText(self, -1, "Exposure bleach compensation (%):")
rowSizer.Add(text, 0, wx.ALL, 5)
## Ordered list of bleach compensation percentages.
self.bleachCompensations, subSizer = guiUtils.makeLightsControls(
self, [str(l.name) for l in self.allLights],
self.settings['bleachCompensations'])
rowSizer.Add(subSizer)
sizer.Add(rowSizer)
# Now a row for the collection order.
rowSizer = wx.BoxSizer(wx.HORIZONTAL)
self.siCollectionOrder = guiUtils.addLabeledInput(
self,
rowSizer,
label="Collection order",
control=wx.Choice(self, choices=sorted(COLLECTION_ORDERS.keys())),
helperString=
"What order to change the angle, phase, and Z step of the experiment. E.g. for \"Angle, Phase, Z\" Angle will change most slowly and Z will change fastest."
)
self.siCollectionOrder.SetSelection(self.settings['siCollectionOrder'])
sizer.Add(rowSizer)
self.SetSizerAndFit(sizer)
def onSelector(self, event):
## TODO: fix focus issue so that key bindings work immediately after camera enable.
## Currently, have to mouse-over the bitmap area, or click in another window.
menu = wx.Menu()
if self.curCamera is not None:
item = menu.Append(-1,
"Disable %s" % self.curCamera.descriptiveName)
self.Bind(wx.EVT_MENU, lambda event: self.curCamera.toggleState(),
item)
menu.InsertSeparator(1)
items = self.canvas.getMenuActions()
for label, action in items:
item = menu.Append(-1, label)
self.Bind(wx.EVT_MENU,
lambda event, action=action: action(),
item)
menu.InsertSeparator(len(items) + 2)
for size in self.curCamera.getImageSizes():
item = menu.Append(-1, "Set image size to %s" % str(size))
self.Bind(
wx.EVT_MENU,
lambda event, size=size: self.curCamera.setImageSize(size),
item)
else:
# Get all inactive cameras.
cameras = depot.getHandlersOfType(depot.CAMERA)
cameras.sort(key=lambda c: c.descriptiveName)
for camera in cameras:
if not camera.getIsEnabled():
item = menu.Append(-1,
"Enable %s" % camera.descriptiveName)
self.Bind(wx.EVT_MENU,
lambda event, cam=camera: cam.toggleState(),
item)
cockpit.gui.guiUtils.placeMenuAtMouse(self, menu)
def save(self):
# First, re-order our images into WTZ order (with the Z axis being
# flat).
numTimes = len(self.timesAndImages)
numCams = len(self.timesAndImages[0][1])
allImages = numpy.zeros((numCams, numTimes, 1, self.maxImageDims[0], self.maxImageDims[1]), dtype = numpy.float32)
exposureTimes = []
for timepoint, (exposureTime, images, rawImages) in enumerate(self.timesAndImages):
for wavelength, image in enumerate(images):
height, width = image.shape
allImages[wavelength, timepoint, 0, :height, :width] = image
exposureTimes.append(exposureTime)
drawer = depot.getHandlersOfType(depot.DRAWER)[0]
header = cockpit.util.datadoc.makeHeaderFor(allImages,
wavelengths = [cam.wavelength for cam in self.cameras])
# Number of bytes allocated to the extended header: 4 per image, since
# we use a 32-bit floating point for the exposure time.
header.next = 4 * numCams * numTimes
header.NumFloats = 1
handle = open(self.savePath, 'wb')
handle.write(header._array.tostring())
exposureTimes = numpy.array(exposureTimes, dtype = numpy.float32)
handle.write(exposureTimes)
handle.write(allImages)
handle.close()
def _getDefaultSettings(self):
allLights = depot.getHandlersOfType(depot.LIGHT_TOGGLE)
default = {
'bleachCompensations': ['' for l in self.allLights],
'siCollectionOrder': 0,
}
return default
def generateTitles(self):
typeToHandlers = {}
# Include light filters for our active lights, even though they aren't
# a part of self.allHandlers.
typeToHandlers[depot.LIGHT_FILTER] = []
filters = depot.getHandlersOfType(depot.LIGHT_FILTER)
for light in self.lights:
wavelength = light.getWavelength()
for filterHandler in filters:
if light in filterHandler.lights:
typeToHandlers[depot.LIGHT_FILTER].append(filterHandler)
for handler in self.allHandlers:
# We don't care about stage positioners because we always include
# the complete stage position anyway.
if handler.deviceType != depot.STAGE_POSITIONER:
if handler.deviceType not in typeToHandlers:
typeToHandlers[handler.deviceType] = []
typeToHandlers[handler.deviceType].append(handler)
titles = [
"Date & time: %s; pos: %s" % (
time.strftime('%Y/%m/%d %H:%M:%S'),
str(['%.2f' % p for p in (cockpit.interfaces.stageMover.getPosition())])
)
]
# Append the metadata we were given to start.
for i in range(0, len(self.metadata) + 80, 80):
substring = self.metadata[i * 80 : (i + 1) * 80]
if substring:
titles.append(substring)
for deviceType, handlers in typeToHandlers.items():
handlers = sorted(handlers, key = lambda a: a.name)
entries = []
for handler in handlers:
text = handler.getSavefileInfo()
if handler in self.lightToExposureTime and self.lightToExposureTime[handler]:
text += ': ' + ','.join(["%.3fms" % t for t in sorted(self.lightToExposureTime[handler]) ])
# Record the exposure duration(s) of the light source.
# find associated power entries (if they have them)
for hand in depot.getHandlersInGroup(handler.groupName):
if hand.deviceType == 'light power':
text += " %3.3f mW" % hand.lastPower
if text:
entries.append(text)
if entries:
entry = "[%s: %s]" % (deviceType, ';'.join(entries))
while len(entry) > 80:
# Must split it across lines.
# \todo For now doing this in an optimally-space-saving
# method that will result in ugly titles since we split
# lines in the middle of a word.
titles.append(entry[:80])
entry = entry[80:]
titles.append(entry)
if len(titles) > 10:
raise RuntimeError("Have too much miscellaneous information to fit into the \"titles\" section of the MRC file (max 10 lines). Lines are:\n%s" % "\n".join(titles))
return titles
def __init__(self):
## List of Handlers capable of taking images.
self.imageHandlers = depot.getHandlersOfType(depot.IMAGER)
## Set of active cameras, so we can check their framerates.
self.activeCameras = set()
events.subscribe(
events.CAMERA_ENABLE,
lambda c, isOn: self.toggle(self.activeCameras, c, isOn))
## Set of active light sources, so we can check their exposure times.
self.activeLights = set()
events.subscribe(
events.LIGHT_SOURCE_ENABLE,
lambda l, isOn: self.toggle(self.activeLights, l, isOn))
## Time of last call to takeImage(), so we can avoid calling it
# faster than the time it takes to actually collect another image.
self.lastImageTime = time.time()
## Boolean to control activity of the video mode thread.
self.shouldStopVideoMode = False
## Boolean that indicates if we're currently in video mode.
self.amInVideoMode = False
events.subscribe('user abort', self.stopVideo)
# Update exposure times on certain events.
events.subscribe('light exposure update', self.updateExposureTime)
events.subscribe(events.LIGHT_SOURCE_ENABLE,
lambda *args: self.updateExposureTime())
events.subscribe(events.CAMERA_ENABLE,
lambda *args: self.updateExposureTime())
def goToSite(uniqueID, shouldBlock = False):
site = mover.idToSite[uniqueID]
objective = depot.getHandlersOfType(depot.OBJECTIVE)[0]
objOffset = objective.getOffset()
offsetPosition=list(site.position)
for i in range(len(offsetPosition)):
offsetPosition[i]=offsetPosition[i]+objOffset[i]
goTo(offsetPosition, shouldBlock)
def disconnect(self):
if self.connection is not None:
server = depot.getHandlersOfType(depot.SERVER)[0]
server.unregister(self.callback)
try:
self.connection.receiveClient(None)
except Exception as e:
print ("Couldn't disconnect from %s: %s" % (self.serviceName, e))
self.connection = None
def cleanUp(self):
for i, shouldActivate in enumerate(self.activeLights):
self.allLights[i].setEnabled(shouldActivate)
if (self.shouldPowerDownWhenDone is not None
and self.shouldPowerDownWhenDone.GetValue()):
handlers = depot.getHandlersOfType(depot.POWER_CONTROL)
for handler in handlers:
handler.disable()
events.publish(events.UPDATE_STATUS_LIGHT, 'device waiting', '')
def connect(self, callback, timeout = 5):
self.callback = callback
connection = Pyro4.Proxy(
'PYRO:%s@%s:%d' % (self.serviceName, self.ipAddress, self.port))
connection._pyroTimeout = timeout
self.connection = connection
server = depot.getHandlersOfType(depot.SERVER)[0]
uri = server.register(self.callback, self.localIp)
self.connection.receiveClient(uri)
def loadSettings(self):
result = cockpit.util.userConfig.getValue(
self.configKey, default=self._getDefaultSettings())
allLights = depot.getHandlersOfType(depot.LIGHT_TOGGLE)
if len(result['bleachCompensations']) != len(self.allLights):
# Number of light sources has changed; invalidate the config.
result['bleachCompensations'] = ['' for light in self.allLights]
return result
def onObjectiveChange(self, name, pixelSize, transform, offset, **kwargs):
h = depot.getHandlersOfType(depot.OBJECTIVE)[0]
self.crosshairBoxSize = 512 * pixelSize
self.offset = offset
self.objectiveSelectedText.SetLabel(name.center(15))
colour = tuple(map(lambda x: 255 * x, h.getColour()))
self.objectiveSelectedText.SetBackgroundColour(colour)
#force a redraw so that the crosshairs are properly sized
self.Refresh()
def __init__(self, parent, size, id=-1, *args, **kwargs):
super().__init__(parent, id, size=size, *args, **kwargs)
## WX context for drawing.
self.context = wx.glcanvas.GLContext(self)
## Whether or not we have done some one-time-only logic.
self.haveInitedGL = False
## Whether or not we should try to draw
self.shouldDraw = True
## Font for drawing text
try:
self.font = ftgl.TextureFont(cockpit.gui.FONT_PATH)
self.font.setFaceSize(18)
except Exception as e:
print("Failed to make font:", e)
## X values below this are off the canvas. We leave it up to children
# to fill in proper values for these.
self.minX = 0
## X values above this are off the canvas
self.maxX = 1000
## Y values below this are off the canvas
self.minY = 0
## Y values above this are off the canvas
self.maxY = 1000
## (X, Y, Z) vector describing the stage position as of the last
# time we drew ourselves. We need this to display motion deltas.
self.prevStagePosition = numpy.zeros(3)
## As above, but for the current position.
self.curStagePosition = numpy.zeros(3)
## Event used to indicate when drawing is done, so we can update
# the above.
self.drawEvent = threading.Event()
##objective offset info to get correct position and limits
self.objective = depot.getHandlersOfType(depot.OBJECTIVE)[0]
self.listObj = list(self.objective.nameToOffset.keys())
self.listOffsets = list(self.objective.nameToOffset.values())
self.offset = self.objective.getOffset()
## Boolean to just force a redraw.
self.shouldForceRedraw = False
## Thread that ensures we don't spam redisplaying ourselves.
self.redrawTimerThread = threading.Thread(target=self.refreshWaiter,
name="macrostage-refresh")
self.redrawTimerThread.start()
self.Bind(wx.EVT_PAINT, self.onPaint)
self.Bind(wx.EVT_SIZE, lambda event: event)
self.Bind(wx.EVT_ERASE_BACKGROUND,
lambda event: event) # Do nothing, to avoid flashing
events.subscribe(events.STAGE_POSITION, self.onMotion)
events.subscribe("stage step index", self.onStepIndexChange)
def disconnect(self):
if not self._listening:
# Nothing to do.
return
server = depot.getHandlersOfType(depot.SERVER)[0]
server.unregister(self._callback)
try:
self._proxy.receiveClient(None)
except Exception as e:
print ("Couldn't disconnect listener from %s: %s" % (self._proxy, e))
self._listening = False
def connect(self, callback=None, timeout=5):
server = depot.getHandlersOfType(depot.SERVER)[0]
if self._listening:
server.unregister(self._callback)
if callback:
self._callback = callback
elif not self._callback:
# No callback specified in either self._callback or this call.
raise Exception('No callback set.')
uri = server.register(self._callback, self._localIp)
self._proxy.receiveClient(uri)
self._listening = True
def __init__(self,
numReps,
repDuration,
imagesPerRep,
sliceHeight=0,
metadata='',
savePath=''):
super().__init__(numReps,
repDuration,
None,
0,
0,
sliceHeight, {},
metadata=metadata,
savePath=savePath)
# Number of images to be collected per camera per rep.
self.imagesPerRep = imagesPerRep
# We didn't pass a proper exposureSettings value when calling
# the Experiment constructor so we must fix the cameras and
# lights attributes, as well as related attributes, ourselves.
## List of cameras. Assume our cameras are all active cameras.
self.cameras = []
for cam in depot.getHandlersOfType(depot.CAMERA):
if cam.getIsEnabled():
self.cameras.append(cam)
## List of light sources. Assume our lights are all active lights.
self.lights = []
for light in depot.getHandlersOfType(depot.LIGHT_TOGGLE):
if light.getIsEnabled():
self.lights.append(light)
self.allHandlers += self.cameras + self.lights
self.lightToExposureTime = {l: set() for l in self.lights}
self.cameraToIsReady = {c: True for c in self.cameras}
self.cameraToImageCount = {c: 0 for c in self.cameras}
self.cameraToIgnoredImageIndices = {c: set() for c in self.cameras}
def centerCanvas(self, event=None):
curPosition = cockpit.interfaces.stageMover.getPosition()[:2]
# Calculate the size of the box at the center of the crosshairs.
# \todo Should we necessarily assume a 512x512 area here?
objective = depot.getHandlersOfType(depot.OBJECTIVE)[0]
#if we havent previously set crosshairBoxSize (maybe no camera active)
if (self.crosshairBoxSize == 0):
self.crosshairBoxSize = 512 * objective.getPixelSize()
self.offset = objective.getOffset()
scale = (150. / self.crosshairBoxSize)
self.canvas.zoomTo(-curPosition[0] + self.offset[0],
curPosition[1] - self.offset[1], scale)
def laserExpUpdate(self, source=None):
# TODO: fix this to use the handler reference passed in source
# i.e. we *do* know which light is update.
#Dont know which light is updated so update them all.
lightToggles = depot.getHandlersOfType(depot.LIGHT_TOGGLE)
lightToggles = sorted(lightToggles, key=lambda l: l.wavelength)
for light in lightToggles:
textString = self.nameToText[light.groupName + 'exp']
label = '%5d ms' % (light.getExposureTime())
textString.SetLabel(label.rjust(10))
self.Refresh()
def __init__(self, parent):
super().__init__(parent)
self.Sizer = wx.BoxSizer(wx.VERTICAL)
self.Sizer.Add(PanelLabel(self, label="Filters"))
subpanel = wx.Panel(self, style=wx.BORDER_RAISED)
self.Sizer.Add(subpanel, 1, wx.EXPAND)
subpanel.Sizer = wx.WrapSizer(orient=wx.VERTICAL)
filters = depot.getHandlersOfType(depot.LIGHT_FILTER)
if not filters:
self.Hide()
return
for i, f in enumerate(filters):
subpanel.Sizer.Add(f.makeUI(subpanel), 0,
wx.EXPAND | wx.RIGHT | wx.BOTTOM, 8)
def initGL(self):
glClearColor(1, 1, 1, 0)
# Non-zero objective offsets require expansion of area covered
# by megatiles.
objs = depot.getHandlersOfType(depot.OBJECTIVE)
offsets = list(
itertools.chain(*[ob.nameToOffset.values() for ob in objs]))
minmax = lambda l: (min(l), max(l))
xOffLim = minmax([-offset[0] for offset in offsets])
yOffLim = minmax([offset[1] for offset in offsets])
(xMin, xMax), (yMin, yMax) = self.stageHardLimits
# Bounds checks ensure we only increase the megatile area, not
# decrease it.
#
# We need to increase upper limit further so we don't miss tiles at
# the edges. We should just need to add megaTileMicronSize to each
# axis to make sure we add tiles up to and including our bounds. This
# works for the x-axis, but not y. For some reason, we need to add
# *four* times this value to the y-axis. The megaTile's y-origin is at
# its centre, whereas the x-origin is at an edge; as far as I can see,
# but this should require only up to three times the tilesize added to
# the upper limit, not four.
# Four works, though.
# Arrays run [0,0] .. [ncols, nrows]; GL runs (-1,-1) .. (1,1). Since
# making adjustments to render [0,0] at (-1,1), we now add two megatiles
# at each y limit, rather than 4 at one edge.
tsize = glGetInteger(GL_MAX_TEXTURE_SIZE)
# If we use the full texture size, it seems it's too large for manipul-
# ation in a framebuffer:
# * on Macs with Intel chipsets;
# * on some mobile nVidia chipsets.
# Check vendor with glGetString(GL_VENDOR)
# GL_MAX_FRAMEBUFFER_WIDTH and _HEIGHT are not universally available,
# so we just use a quarter of the max texture size or a reasonable
# upper bound which has been found to work in tests on 2017-ish
# Macbook Pro.
tsize = min(tsize // 4, 16384)
MegaTile.setPixelSize(tsize)
xMin += min(0, xOffLim[0]) - MegaTile.micronSize
xMax += max(0, xOffLim[1]) + MegaTile.micronSize
yMin += min(0, yOffLim[0]) - 2 * MegaTile.micronSize
yMax += max(0, yOffLim[1]) + 2 * MegaTile.micronSize
for x in np.arange(xMin, xMax, MegaTile.micronSize):
for y in np.arange(yMin, yMax, MegaTile.micronSize):
self.megaTiles.append(MegaTile((-x, y)))
self.haveInitedGL = True
def onSelector(self, event):
## TODO: fix focus issue so that key bindings work immediately after camera enable.
## Currently, have to mouse-over the bitmap area, or click in another window.
menu = wx.Menu()
if self.curCamera is not None:
item = menu.Append(-1, "Disable %s" % self.curCamera.descriptiveName)
self.Bind(wx.EVT_MENU, lambda event: self.curCamera.toggleState(), item)
else:
# Get all inactive cameras.
cameras = depot.getHandlersOfType(depot.CAMERA)
cameras.sort(key = lambda c: c.descriptiveName)
for camera in cameras:
if not camera.getIsEnabled():
item = menu.Append(-1, "Enable %s" % camera.descriptiveName)
self.Bind(wx.EVT_MENU,
lambda event, cam=camera: cam.toggleState(), item)
cockpit.gui.guiUtils.placeMenuAtMouse(self, menu)
def onMouse(self, event):
if event.LeftDClick():
if self.imagePos is None:
self.imagePos = cockpit.interfaces.stageMover.getPosition()
x, y = event.GetPosition()
pixelSize = depot.getHandlersOfType(
depot.OBJECTIVE)[0].getPixelSize()
x0, y0 = self.canvas.glToIndices(0, 0)
dy, dx = self.canvas.canvasToIndices(x, y)
dx -= x0
dy -= y0
dx *= pixelSize
dy *= pixelSize
target = (self.imagePos[0] - dx, self.imagePos[1] + dy)
cockpit.interfaces.stageMover.goToXY(target)
else:
event.Skip()
def changeObjective(self):
# If we have only two objectives, then simply flip them
h = depot.getHandlersOfType(depot.OBJECTIVE)[0]
if (h.numObjectives == 2):
for obj in h.sortedObjectives:
if h.currentObj != obj:
h.changeObjective(obj)
break
else:
# More than 2 objectives so need to present a list.
menu = wx.Menu()
for i, objective in enumerate(h.sortedObjectives):
menu.Append(i, objective)
menu.Bind(wx.EVT_MENU,
lambda evt, obj=objective: h.changeObjective(obj),
id=i)
cockpit.gui.guiUtils.placeMenuAtMouse(self.panel, menu)
def __init__(self, parent):
super().__init__(parent)
self.Sizer = wx.BoxSizer(wx.VERTICAL)
label = PanelLabel(self, label="Objective")
self.Sizer.Add(label)
panel = wx.Panel(self, style=wx.RAISED_BORDER)
self.Sizer.Add(panel, 1, wx.EXPAND)
panel.Sizer = wx.BoxSizer(wx.VERTICAL)
for o in depot.getHandlersOfType(depot.OBJECTIVE):
ctrl = wx.Choice(panel)
ctrl.Set(o.sortedObjectives)
panel.Sizer.Add(ctrl)
ctrl.Bind(wx.EVT_CHOICE,
lambda evt: o.changeObjective(evt.GetString()))
events.subscribe(
"objective change",
lambda *a, **kw: ctrl.SetSelection(ctrl.FindString(a[0])))
def OnStart(self, evt):
self.saveSettings()
curLoc = cockpit.interfaces.stageMover.getPosition()
imageWidth = float(self.imageWidth.GetValue())
imageHeight = float(self.imageHeight.GetValue())
markerSize = float(self.markerSize.GetValue())
objective = depot.getHandlersOfType(depot.OBJECTIVE)[0]
pixelSize = objective.getPixelSize()
for xOffset in range(int(self.numColumns.GetValue())):
xLoc = curLoc[0] - xOffset * pixelSize * imageWidth
for yOffset in range(int(self.numRows.GetValue())):
yLoc = curLoc[1] - yOffset * pixelSize * imageHeight
target = numpy.array([xLoc, yLoc, curLoc[2]])
newSite = cockpit.interfaces.stageMover.Site(target,
size=markerSize)
cockpit.interfaces.stageMover.saveSite(newSite)
self.Destroy()
def __init__(self, parent):
super().__init__(parent)
self.Sizer = wx.BoxSizer(wx.VERTICAL)
sz = wx.BoxSizer(wx.HORIZONTAL)
label = PanelLabel(self, label="Cameras")
self.Sizer.Add(label)
self.Sizer.Add(sz)
cameras = sorted(depot.getHandlersOfType(depot.CAMERA),
key=lambda c: c.name)
self.panels = {}
for cam in cameras:
panel = CameraPanel(self, cam)
sz.Add(panel, flag=wx.EXPAND)
self.panels[cam] = panel
sz.AddSpacer(4)
self.Fit()