def _run_interface(self, runtime):
petavgfile = self.inputs.petavgfile
mriregfile = self.inputs.mriregfile
mrireg = nib.load(mriregfile)
petavg = nib.load(petavgfile)
_, base, _ = split_filename(petavgfile)
# Visualize the overlaid PiB 20-min average and the coregistered MRI
p = regtools.overlay_slices(petavg.get_data(), mrireg.get_data(),
None, 0, "PET", "Coregistered MRI",
fname=base+'_coreg_overlay_sagittal.png')
p = regtools.overlay_slices(petavg.get_data(), mrireg.get_data(),
None, 1, "PET", "Coregistered MRI",
fname=base+'_coreg_overlay_coronal.png')
p = regtools.overlay_slices(petavg.get_data(), mrireg.get_data(),
None, 2, "PET", "Coregistered MRI",
fname=base+'_coreg_overlay_axial.png')
fig = plt.figure(figsize=(15,5))
display = plot_anat(petavgfile,figure=fig)
display.add_edges(mriregfile)
display.title('MRI edges on PET')
fig.savefig(base+'_coreg_edges.png', format='png')
return runtime
def exampleDipy():
# example obtained from: http://nipy.org/dipy/examples_built/syn_registration_2d.html
import ssl
if hasattr(ssl, '_create_unverified_context'):
ssl._create_default_https_context = ssl._create_unverified_context
from dipy.data import fetch_stanford_hardi, read_stanford_hardi
fetch_stanford_hardi()
nib_stanford, gtab_stanford = read_stanford_hardi()
stanford_b0 = np.squeeze(nib_stanford.get_data())[..., 0]
from dipy.data.fetcher import fetch_syn_data, read_syn_data
fetch_syn_data()
nib_syn_t1, nib_syn_b0 = read_syn_data()
syn_b0 = np.array(nib_syn_b0.get_data())
from dipy.segment.mask import median_otsu
stanford_b0_masked, stanford_b0_mask = median_otsu(stanford_b0, 4, 4)
syn_b0_masked, syn_b0_mask = median_otsu(syn_b0, 4, 4)
static = stanford_b0_masked
static_affine = nib_stanford.affine
moving = syn_b0_masked
moving_affine = nib_syn_b0.affine
pre_align = np.array(
[[1.02783543e+00, -4.83019053e-02, -6.07735639e-02, -2.57654118e+00],
[4.34051706e-03, 9.41918267e-01, -2.66525861e-01, 3.23579799e+01],
[5.34288908e-02, 2.90262026e-01, 9.80820307e-01, -1.46216651e+01],
[0.00000000e+00, 0.00000000e+00, 0.00000000e+00, 1.00000000e+00]])
from dipy.align.imaffine import AffineMap
affine_map = AffineMap(pre_align,
static.shape, static_affine,
moving.shape, moving_affine)
resampled = affine_map.transform(moving)
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(static, moving, static_affine, moving_affine,
pre_align)
warped_moving = mapping.transform(moving)
for slice in range(41 - 12, 41 + 13):
regtools.overlay_slices(static, resampled, slice, 1, 'Static',
'Pre Moving',
'GIFexample1/' + str(slice) + 'T1pre.png')
regtools.overlay_slices(static, warped_moving, slice, 1, 'Static',
'Post moving',
'GIFexample1/' + str(slice) + 'T1post.png')
def multiDimOverlayPlot(xSlice,ySlice,zSlice):
#import dipy regtools
from dipy.viz import regtools
#import matplotlib functions
image1=t1Resampled
image2=atlasImg
import matplotlib.pyplot as plt
from matplotlib.pyplot import imshow
sagitalFig=regtools.overlay_slices(image1.get_fdata(),image2.get_fdata(),slice_index=xSlice, slice_type=0)
coronalFig=regtools.overlay_slices(image1.get_fdata(),image2.get_fdata(),slice_index=ySlice, slice_type=1)
axialFig=regtools.overlay_slices(image1.get_fdata(),image2.get_fdata(),slice_index=zSlice, slice_type=2)
def abstractExample():
numBlobs = 4
shape = np.array([81, 106, 76])
static = np.zeros(shape)
resampled = np.zeros(shape)
size_blob = 3
for blob in range(numBlobs):
jitter = np.random.randint(5)
randIndX = 41 + 2 * blob
randIndY = np.random.randint(shape[1] - 2 * size_blob)
randIndZ = np.random.randint(shape[2] - 2 * size_blob)
static[randIndX - size_blob:randIndX + size_blob,
randIndY - size_blob:randIndY + size_blob,
randIndZ - size_blob:randIndZ + size_blob] = 1
randIndY = randIndY + jitter
randIndZ = randIndZ + jitter + np.random.randint(-1, 2)
resampled[randIndX - size_blob:randIndX + size_blob,
randIndY - size_blob:randIndY + size_blob,
randIndZ - size_blob:randIndZ + size_blob] = 1
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(static, resampled)
warped_moving = mapping.transform(resampled)
for slice in range(41 - 3 * size_blob, 41 + 3 * size_blob + 1):
regtools.overlay_slices(static, resampled, slice, 0, 'Static',
'Pre Moving',
'GIFexample1/' + str(slice) + 'preMov.png')
regtools.overlay_slices(static, warped_moving, slice, 0, 'Static',
'Post moving',
'GIFexample1/' + str(slice) + 'postMov.png')
def overlay_3d_images(static, transformed):
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_affine_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_affine_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_affine_2.png")
def reg_func(figname, static_mask=None, moving_mask=None):
"""Convenience function for registration using a pipeline.
Uses variables in global scope, except for static_mask and moving_mask.
"""
pipeline = [translation, rigid]
xformed_img, reg_affine = affine_registration(moving,
static,
moving_affine=moving_affine,
static_affine=static_affine,
nbins=32,
metric='MI',
pipeline=pipeline,
level_iters=level_iters,
sigmas=sigmas,
factors=factors,
static_mask=static_mask,
moving_mask=moving_mask)
regtools.overlay_slices(static, xformed_img, None, 2, "Static",
"Transformed", figname)
return
def align_atlas():
neo_fname = get_neobrain('train', 1, 'T2')
neo_nib = nib.load(neo_fname)
neo = neo_nib.get_data()
neo_affine = neo_nib.get_affine()
# Load atlas (with skull)
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas = atlas_nib.get_data()
atlas_affine = atlas_nib.get_affine()
# The first training volume dimensions are about 5cm x 5cm x 8cm
# The atlas dimensions are about 7cm x 9cm x 11 cm
# Assuming isotropic scale, the atlas is about 1.5 times larger than
# the input image:
iso_scale = (float(7*9*11)/float(5*5*8))**(1.0/3)
print(iso_scale)
#We can use this to constraint the transformation to rigid
scale = np.eye(4)
scale[:3,:3] *= iso_scale
rigid_map_fname = 'atlas_towards_neo1_rigid.p'
if os.path.isfile(rigid_map_fname):
rigid_map = pickle.load(open(rigid_map_fname, 'r'))
else:
transforms = ['RIGID']
rigid_map = dipy_align(neo, neo_affine, atlas, atlas_affine,
transforms=transforms, prealign=scale)
pickle.dump(rigid_map, open(rigid_map_fname, 'w'))
atlas_resampled = rigid_map.transform(atlas)
# Compare anterior coronal slices
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=10, ltitle='Neo1', rtitle='Atlas');
# Compare middle coronal slices
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=25, ltitle='Neo1', rtitle='Atlas');
# Compare posterior coronal slices
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=40, ltitle='Neo1', rtitle='Atlas');
# Load the peeled atlas
atlas_wcerebellum_fname = get_neobrain('atlas', 'neo-withCerebellum', None)
atlas_wcerebellum_nib = nib.load(atlas_wcerebellum_fname)
atlas_wcerebellum = atlas_wcerebellum_nib.get_data()
atlas_wcerebellum_affine = atlas_wcerebellum_nib.get_affine()
# Configure diffeomorphic registration
diff_map_name = 'atlas_towards_neo1_diff.p'
if os.path.isfile(diff_map_name):
diff_map = pickle.load(open(diff_map_name, 'r'))
else:
metric = CCMetric(3)
sdr = SymmetricDiffeomorphicRegistration(metric)
# The atlases are not aligned in physical space!! use atlas_affine instead of atlas_wcerebellum_affine
diff_map = sdr.optimize(neo, atlas_wcerebellum, neo_affine, atlas_affine, prealign=rigid_map.affine)
pickle.dump(diff_map, open(diff_map_name, 'w'))
atlas_wcerebellum_deformed = diff_map.transform(atlas_wcerebellum)
# Before and after diffeomorphic registration
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=10, ltitle='Neo1', rtitle='Atlas');
rt.overlay_slices(neo, atlas_wcerebellum_deformed, slice_type=2, slice_index=10, ltitle='Neo1', rtitle='Atlas');
# Before and after diffeomorphic registration
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=25, ltitle='Neo1', rtitle='Atlas');
rt.overlay_slices(neo, atlas_wcerebellum_deformed, slice_type=2, slice_index=25, ltitle='Neo1', rtitle='Atlas');
# Before and after diffeomorphic registration
rt.overlay_slices(neo, atlas_resampled, slice_type=2, slice_index=40, ltitle='Neo1', rtitle='Atlas');
rt.overlay_slices(neo, atlas_wcerebellum_deformed, slice_type=2, slice_index=40, ltitle='Neo1', rtitle='Atlas');
# Now all volumes
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas = atlas_nib.get_data()
atlas_affine = atlas_nib.get_affine()
atlas_wcerebellum_fname = get_neobrain('atlas', 'neo-withCerebellum', None)
atlas_wcerebellum_nib = nib.load(atlas_wcerebellum_fname)
atlas_wcerebellum = atlas_wcerebellum_nib.get_data()
atlas_wcerebellum_affine = atlas_wcerebellum_nib.get_affine()
idx = 2
neoi_fname = get_neobrain('train', idx, 'T2')
neoi_nib = nib.load(neoi_fname)
neoi = neoi_nib.get_data()
neoi_affine = neoi_nib.get_affine()
iso_scale = (float(7*9*11)/float(5*5*8))**(1.0/3)
print(iso_scale)
#We can use this to constraint the transformation to rigid
scale = np.eye(4)
scale[:3,:3] *= iso_scale
rigid_map_fname = 'atlas_towards_neo%d_affine.p'%(idx,)
if os.path.isfile(rigid_map_fname):
rigid_map = pickle.load(open(rigid_map_fname, 'r'))
else:
transforms = ['RIGID', 'AFFINE']
level_iters = [[10000, 1000, 100], [100]]
rigid_map = dipy_align(neoi, neoi_affine, atlas, atlas_affine,
transforms=transforms,
level_iters=level_iters,
prealign=scale)
pickle.dump(rigid_map, open(rigid_map_fname, 'w'))
atlas_resampled = rigid_map.transform(atlas)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index = 6)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index = 10)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index = 25)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index = 40)
diff_map_name = 'atlas_towards_neo%d_diff.p'%(idx,)
if os.path.isfile(diff_map_name):
diff_map = pickle.load(open(diff_map_name, 'r'))
else:
metric = CCMetric(3)
sdr = SymmetricDiffeomorphicRegistration(metric)
# The atlases are not aligned in physical space!! use atlas_affine instead of atlas_wcerebellum_affine
diff_map = sdr.optimize(neoi, atlas_wcerebellum, neoi_affine, atlas_affine, prealign=rigid_map.affine)
pickle.dump(diff_map, open(diff_map_name, 'w'))
atlas_wcerebellum_deformed = diff_map.transform(atlas_wcerebellum)
# Before and after diffeomorphic registration
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=6, ltitle='Neo%d'%(idx,), rtitle='Atlas');
rt.overlay_slices(neoi, atlas_wcerebellum_deformed, slice_type=2, slice_index=6, ltitle='Neo%d'%(idx,), rtitle='Atlas');
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=10, ltitle='Neo%d'%(idx,), rtitle='Atlas');
rt.overlay_slices(neoi, atlas_wcerebellum_deformed, slice_type=2, slice_index=10, ltitle='Neo%d'%(idx,), rtitle='Atlas');
# Before and after diffeomorphic registration
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=25, ltitle='Neo%d'%(idx,), rtitle='Atlas');
rt.overlay_slices(neoi, atlas_wcerebellum_deformed, slice_type=2, slice_index=25, ltitle='Neo%d'%(idx,), rtitle='Atlas');
# Before and after diffeomorphic registration
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=40, ltitle='Neo%d'%(idx,), rtitle='Atlas');
rt.overlay_slices(neoi, atlas_wcerebellum_deformed, slice_type=2, slice_index=40, ltitle='Neo%d'%(idx,), rtitle='Atlas');
def registration_proxy(in_file, static, out_file):
"""
http://nipy.org/dipy/examples_built/affine_registration_3d.html
in_file --> moving
static and moving = path
"""
import time
import numpy as np
import nibabel as nb
import matplotlib.pyplot as plt
from dipy.viz import regtools
from dipy.align.imaffine import (transform_centers_of_mass, AffineMap,
MutualInformationMetric,
AffineRegistration)
from dipy.align.transforms import (TranslationTransform3D,
RigidTransform3D, AffineTransform3D)
t0_time = time.time()
print('---> I. Translation of the moving image towards the static image')
#condition if we have a path or a nifti file
static_img = nb.load(static)
static = static_img.get_data()
static_grid2world = static_img.affine
moving_img = nb.load(in_file)
moving = np.array(moving_img.get_data())[..., 0]
moving_grid2world = moving_img.affine
# resample for have the same number of voxels
print(
'---> Resembling the moving image on a grid of the same dimensions as the static image'
)
identity = np.eye(4)
affine_map = AffineMap(identity, static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0, "Static", "Moving",
"resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1, "Static", "Moving",
"resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2, "Static", "Moving",
"resampled_2.png")
plt.show()
#centers of mass registration
print('---> Aligning the centers of mass of the two images')
c_of_mass = transform_centers_of_mass(static, static_grid2world, moving,
moving_grid2world)
transformed = c_of_mass.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_com_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_com_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_com_2.png")
plt.show()
print('---> II. Refine by looking for an affine transform')
#affine transform
#parameters??
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10000, 1000, 100]
sigmas = [3.0, 1.0, 0.0]
factors = [4, 2, 1]
print('---> Computing Affine Registration (non-convex optimization)')
affreg = AffineRegistration(metric=metric,
level_iters=level_iters,
sigmas=sigmas,
factors=factors)
transform = TranslationTransform3D()
params0 = None
starting_affine = c_of_mass.affine
translation = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = translation.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_trans_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_trans_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_trans_2.png")
plt.show()
print('--->III. Refining with a rigid transform')
#rigid transform
transform = RigidTransform3D()
params0 = None
starting_affine = translation.affine
rigid = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = rigid.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_rigid_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_rigid_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_rigid_2.png")
plt.show()
print(
'--->IV. Refining with a full afine transform (translation, rotation, scale and shear)'
)
#full affine transform
transform = AffineTransform3D()
params0 = None
starting_affine = rigid.affine
affine = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = affine.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_affine_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_affine_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_affine_2.png")
plt.show()
# Save the new data in a new NIfTI image
nb.Nifti1Image(transformed, static_img.affine).to_filename(out_file)
#name = os.path.splitext(basename(moving_path))[0] + '_affine_reg'
#nib.save(nib.Nifti1Image(transformed, np.eye(4)), name)
t1_time = time.time()
total_time = t1_time - t0_time
print('Total time:' + str(total_time))
print('Translated file now is here: %s' % out_file)
return print('Successfully affine registration applied')
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(static, moving, static_affine, moving_affine,
highres_map.affine)
warped_moving = mapping.transform(moving)
"""
We show the registration result with:
"""
from dipy.viz import regtools
regtools.overlay_slices(static, warped_moving, None, 0, 'Static', 'Moving',
'transformed_sagittal.png')
regtools.overlay_slices(static, warped_moving, None, 1, 'Static', 'Moving',
'transformed_coronal.png')
regtools.overlay_slices(static, warped_moving, None, 2, 'Static', 'Moving',
'transformed_axial.png')
"""
.. figure:: transformed_sagittal.png
:align: center
.. figure:: transformed_coronal.png
:align: center
.. figure:: transformed_axial.png
:align: center
Deformable registration result.
"""
def test_density_sampling():
import os
import os.path
import numpy as np
import experiments.registration.dataset_info as info
import nibabel as nib
import dipy.align.metrics as metrics
import dipy.align.imwarp as imwarp
from dipy.align import VerbosityLevels
from experiments.registration.rcommon import getBaseFileName, decompose_path, readAntsAffine
from dipy.fixes import argparse as arg
from experiments.registration.evaluation import (
compute_densities,
sample_from_density,
create_ss_de,
create_ss_mode,
create_ss_median,
create_ss_mean,
)
from experiments.registration.splines import CubicSpline
import dipy.viz.regtools as rt
i1_name = info.get_ibsr(1, "strip")
i1_nib = nib.load(i1_name)
i1 = i1_nib.get_data().squeeze()
mask = (i1 > 0).astype(np.int32)
wt1_name = "warpedDiff_brainweb_t2_strip_IBSR_01_ana_strip.nii.gz"
wt1_nib = nib.load(wt1_name)
wt1 = wt1_nib.get_data().squeeze()
rt.overlay_slices(i1, wt1)
nbins = 100
densities = compute_densities(i1.astype(np.int32), wt1.astype(np.float64), nbins, mask)
figure()
imshow(densities)
# Compare different estimators
ss_sampled = create_ss_de(i1.astype(np.int32), densities)
ss_mode = create_ss_mode(i1.astype(np.int32), densities)
ss_median = create_ss_median(i1.astype(np.int32), densities)
ss_mean = create_ss_mean(i1.astype(np.int32), densities)
rt.overlay_slices(ss_sampled, i1)
rt.overlay_slices(ss_mode, i1)
rt.overlay_slices(ss_median, i1)
rt.overlay_slices(ss_mean, i1)
s1 = ss_sampled[:, ss_sampled.shape[1] // 2, :].T
s2 = ss_mode[:, ss_mode.shape[1] // 2, :].T
s3 = ss_median[:, ss_median.shape[1] // 2, :].T
s4 = ss_mean[:, ss_mean.shape[1] // 2, :].T
slices = [[s1, s2], [s3, s4]]
titles = [["Sampled", "Mode"], ["Median", "Mean"]]
fig, ax = plt.subplots(2, 2)
fig.set_facecolor("white")
for ii, a_row in enumerate(ax):
for jj, a in enumerate(a_row):
a.set_axis_off()
a.imshow(slices[ii][jj], cmap=cm.gray, origin="lower")
a.set_title(titles[ii][jj])
# Fit densities with splines in a regular grid
f = densities[100]
kspacing = 1 # Number of grid cells between spline knots
spline = CubicSpline(kspacing)
coef = spline.fit_to_data(f)
# Check fit
fit = spline.evaluate(coef, nbins)
figure()
plot(f)
plot(fit)
# And the derivative
df = spline.evaluate(coef, nbins, 1)
figure()
plot(f)
plot(df)
fit = np.zeros_like(densities)
for i in range(densities.shape[0]):
coef = spline.fit_to_data(densities[i])
fit[i, :] = spline.evaluate(coef, nbins)
fig = plt.figure()
ax = fig.add_subplot(1, 2, 1)
ax.imshow(densities)
ax = fig.add_subplot(1, 2, 2)
ax.imshow(fit)
brainweb_name = info.get_brainweb('t1', 'raw')
brainweb_nib = nib.load(brainweb_name)
brainweb = brainweb_nib.get_data().squeeze()
brainweb_affine = brainweb_nib.get_affine()
brainweb = brainweb.transpose([0, 2, 1])[::-1, :, :]
rt.plot_slices(brainweb)
brainweb_affine = ibsr1_affine.copy()
brainweb_affine[brainweb_affine != 0] = 1
brainweb_affine[0, 0] = -1
# Reslice Brainweb on IBSR1
ibsr_to_bw = AffineMap(None, ibsr1.shape, ibsr1_affine, brainweb.shape,
brainweb_affine)
bw_on_ibsr1 = ibsr_to_bw.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1) # misaligned
c_of_mass = transform_centers_of_mass(ibsr1, ibsr1_affine, brainweb,
brainweb_affine)
bw_on_ibsr1 = c_of_mass.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1) # roughly aligned
# Start affine alignment
aff_name = 'ibsr1_to_brainweb.p'
if os.path.isfile(aff_name):
ibsr_bw_affmap = pickle.load(open(aff_name, 'r'))
else:
ibsr_bw_affmap = dipy_align(ibsr1, ibsr1_affine, brainweb, brainweb_affine)
pickle.dump(ibsr_bw_affmap, open(aff_name, 'w'))
bw_on_ibsr1 = ibsr_bw_affmap.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1, slice_type=0) # aligned (sagital view)
def correlation_metric(fixed, moving):
correlation = pearsonr(fixed.get_data().ravel(),
moving.get_data().ravel())[0]
return correlation
# Try out on a sample subject across two datasets
fixed = nib.load(glob(join('haxby', 'life', 'sub-rid000041', 'anat', '*T1w.nii.gz'))[0])
moving = nib.load(glob(join('haxby', 'attention', 'sub-rid000041', 'anat', '*T1w.nii.gz'))[0])
# Resample moving image if different geometry
if moving.shape != fixed.shape:
moving = resample(fixed, moving)
# Transform moving into to the target fixed image
transformed = rigid_registration(fixed, moving,
level_iters=[100, 10, 5],
sigmas=[3.0, 1.0, 0.0],
factors=[4, 2, 1])
# Evaluate before and after correlation of images
pre = correlation_metric(fixed, moving)
post = correlation_metric(fixed, transformed)
print(("Pre-alignment correlation: {0} \n" +
"Post-alignment correlation: {1}").format(
pre, post))
# Visualize the before/after images
regtools.overlay_slices(fixed.get_data(), moving.get_data(), None, 2,
"Fixed", "Moving")
regtools.overlay_slices(fixed.get_data(), transformed.get_data(), None, 2,
"Fixed", "Transformed")
# 1.2.1 - Rigid Registration of T1 subject image to T2 subject image
# ---------------------------------------------------------------------------------------------
start_time = time.time()
rigidTransformName = t2CurrentSubjectName[0:(t2CurrentSubjectName.index("/T2"))] +"/T1_towards_T2_rigid.p"
rigidTransform = preprocessing.rigidRegister(np.asarray(t2CurrentSubject_data,dtype=np.double), np.asarray(t1CurrentSubject_data,dtype=np.double), \
t2CSAffine, t1CSAffine, None, None, rigidTransformName, 1)
print "Rigid register: {} seconds.".format(time.time() - start_time)
t1CurrentSubject_data1 = rigidTransform.transform(t1CurrentSubject_data)
rt.overlay_slices(t2CurrentSubject_data, t1CurrentSubject_data1, slice_type=2, slice_index=25, ltitle='T2 Subject', rtitle='T1 Subject', fname= results_dir + 'PREP_T1_to_T2_rigid_coronal_slice25.png')
T1CS = nib.Nifti1Image(t1CurrentSubject_data1,t2CSAffine)
nib.save(T1CS,t2CurrentSubjectName[0:(t2CurrentSubjectName.index("/T2"))]+"/T1_1-2.nii.gz")
del t1CurrentSubject_data1
del T1CS
del t1CSAffine
# ---------------------------------------------------------------------------------------------
# 1.2.2 - Rigid Registration # 1 of training subjects to T2
# ---------------------------------------------------------------------------------------------
t2CurrentSubjectName = base_dir + middir1 +neo_subject+ 'T2_1-1.nii.gz'
t1CurrentSubjectName = base_dir + middir1 +neo_subject+ 'T1_1-2.nii.gz'
from dipy.data.fetcher import fetch_syn_data, read_syn_data
fetch_syn_data()
nib_syn_t1, nib_syn_b0 = read_syn_data()
syn_b0 = np.array(nib_syn_b0.get_data())
from dipy.segment.mask import median_otsu
data_b0_masked, data_b0_mask = median_otsu(data_b0, 4, 4)
syn_b0_masked, syn_b0_mask = median_otsu(syn_b0, 4, 4)
static = data_b0_masked
static_affine = nib_stanford.get_affine()
moving = syn_b0_masked
moving_affine = nib_syn_b0.get_affine()
pre_align = np.array(
[[1.02783543e+00, -4.83019053e-02, -6.07735639e-02, -2.57654118e+00],
[4.34051706e-03, 9.41918267e-01, -2.66525861e-01, 3.23579799e+01],
[5.34288908e-02, 2.90262026e-01, 9.80820307e-01, -1.46216651e+01],
[0.00000000e+00, 0.00000000e+00, 0.00000000e+00, 1.00000000e+00]])
import dipy.align.vector_fields as vfu
transform = np.linalg.inv(moving_affine).dot(pre_align.dot(static_affine))
resampled = vfu.warp_3d_affine(moving.astype(np.float32),
np.asarray(static.shape, dtype=np.int32),
transform)
resampled = np.asarray(resampled)
regtools.overlay_slices(static, resampled, None, 1, 'Static', 'Moving',
'input_3d.png')
def wm_syn(template_path, fa_path, working_dir):
"""
A function to perform ANTS SyN registration
Parameters
----------
template_path : str
File path to the template reference image.
fa_path : str
File path to the FA moving image.
working_dir : str
Path to the working directory to perform SyN and save outputs.
"""
import uuid
from time import strftime
from dipy.align.imaffine import MutualInformationMetric, AffineRegistration, transform_origins
from dipy.align.transforms import TranslationTransform3D, RigidTransform3D, AffineTransform3D
from dipy.align.imwarp import SymmetricDiffeomorphicRegistration
from dipy.align.metrics import CCMetric
from dipy.viz import regtools
fa_img = nib.load(fa_path)
template_img = nib.load(template_path)
static = np.asarray(template_img.dataobj)
static_affine = template_img.affine
moving = np.asarray(fa_img.dataobj).astype(np.float32)
moving_affine = fa_img.affine
affine_map = transform_origins(static, static_affine, moving,
moving_affine)
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10, 10, 5]
sigmas = [3.0, 1.0, 0.0]
factors = [4, 2, 1]
affine_reg = AffineRegistration(metric=metric,
level_iters=level_iters,
sigmas=sigmas,
factors=factors)
transform = TranslationTransform3D()
params0 = None
translation = affine_reg.optimize(static, moving, transform, params0,
static_affine, moving_affine)
transform = RigidTransform3D()
rigid_map = affine_reg.optimize(static,
moving,
transform,
params0,
static_affine,
moving_affine,
starting_affine=translation.affine)
transform = AffineTransform3D()
# We bump up the iterations to get a more exact fit:
affine_reg.level_iters = [1000, 1000, 100]
affine_opt = affine_reg.optimize(static,
moving,
transform,
params0,
static_affine,
moving_affine,
starting_affine=rigid_map.affine)
# We now perform the non-rigid deformation using the Symmetric Diffeomorphic Registration(SyN) Algorithm:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(static, moving, static_affine, moving_affine,
affine_opt.affine)
warped_moving = mapping.transform(moving)
# Save warped FA image
run_uuid = '%s_%s' % (strftime('%Y%m%d_%H%M%S'), uuid.uuid4())
warped_fa = '{}/warped_fa_{}.nii.gz'.format(working_dir, run_uuid)
nib.save(nib.Nifti1Image(warped_moving, affine=static_affine), warped_fa)
# We show the registration result with:
regtools.overlay_slices(
static, warped_moving, None, 0, "Static", "Moving",
"%s%s%s%s" % (working_dir, "/transformed_sagittal_", run_uuid, ".png"))
regtools.overlay_slices(
static, warped_moving, None, 1, "Static", "Moving",
"%s%s%s%s" % (working_dir, "/transformed_coronal_", run_uuid, ".png"))
regtools.overlay_slices(
static, warped_moving, None, 2, "Static", "Moving",
"%s%s%s%s" % (working_dir, "/transformed_axial_", run_uuid, ".png"))
return mapping, affine_map, warped_fa
rt.overlay_slices(static, moving, None, 2, "Static", "Warped LCC")
affine_lcc = register("LCC", static, moving, static_grid2space, moving_grid2space)
warped_lcc = transform_image(static, static_grid2space, moving, moving_grid2space, affine_lcc)
rt.overlay_slices(static, warped_lcc, None, 0, "Static", "Warped LCC")
rt.overlay_slices(static, warped_lcc, None, 1, "Static", "Warped LCC")
rt.overlay_slices(static, warped_lcc, None, 2, "Static", "Warped LCC")
return affine_lcc
w_8_32 = transform_image(dwi[..., 8], dwi_nib.get_affine(), dwi[..., 32], dwi_nib.get_affine(), lcc_8_32)
w_8_1 = transform_image(dwi[..., 8], dwi_nib.get_affine(), dwi[..., 1], dwi_nib.get_affine(), lcc_8_1)
w_1_32 = transform_image(dwi[..., 1], dwi_nib.get_affine(), dwi[..., 32], dwi_nib.get_affine(), lcc_1_32)
wp_8_32 = transform_image(dwi[..., 8], dwi_nib.get_affine(), dwi[..., 32], dwi_nib.get_affine(), lcc_8_1.dot(lcc_1_32))
wrong = transform_image(dwi[..., 8], dwi_nib.get_affine(), dwi[..., 32], dwi_nib.get_affine(), lcc_1_32.dot(lcc_8_1))
rt.overlay_slices(dwi[..., 8], dwi[..., 32], None, 2, "Static", "Moving")
rt.overlay_slices(dwi[..., 8], w_8_32, None, 2, "Static", "W-direct")
rt.overlay_slices(dwi[..., 8], wp_8_32, None, 2, "Static", "W-path")
rt.overlay_slices(dwi[..., 8], wrong, None, 2, "Static", "Wrong")
rt.overlay_slices(wp_8_32, wrong, None, 2, "Static", "Wrong")
# dwi_fname = 'usmcuw_dipy.nii.gz'
# B_name = 'B_dipy.txt'
dwi_fname = "Diffusion.nii.gz"
B_name = "B.txt"
dwi_nib = nib.load(dwi_fname)
dwi = dwi_nib.get_data().squeeze()
B = np.loadtxt(B_name)
n = B.shape[0]
def wm_syn(template_path, fa_path, working_dir):
"""A function to perform ANTS SyN registration using dipy functions
Parameters
----------
template_path : str
File path to the template reference FA image.
fa_path : str
File path to the FA moving image (image to be fitted to reference)
working_dir : str
Path to the working directory to perform SyN and save outputs.
Returns
-------
DiffeomorphicMap
An object that can be used to register images back and forth between static (template) and moving (FA) domains
AffineMap
An object used to transform the moving (FA) image towards the static image (template)
"""
fa_img = nib.load(fa_path)
template_img = nib.load(template_path)
static = template_img.get_data()
static_affine = template_img.affine
moving = fa_img.get_data().astype(np.float32)
moving_affine = fa_img.affine
affine_map = transform_origins(static, static_affine, moving,
moving_affine)
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10, 10, 5]
sigmas = [3.0, 1.0, 0.0]
factors = [4, 2, 1]
affine_reg = AffineRegistration(metric=metric,
level_iters=level_iters,
sigmas=sigmas,
factors=factors)
transform = TranslationTransform3D()
params0 = None
translation = affine_reg.optimize(static, moving, transform, params0,
static_affine, moving_affine)
transform = RigidTransform3D()
rigid_map = affine_reg.optimize(
static,
moving,
transform,
params0,
static_affine,
moving_affine,
starting_affine=translation.affine,
)
transform = AffineTransform3D()
# We bump up the iterations to get a more exact fit:
affine_reg.level_iters = [1000, 1000, 100]
affine_opt = affine_reg.optimize(
static,
moving,
transform,
params0,
static_affine,
moving_affine,
starting_affine=rigid_map.affine,
)
# We now perform the non-rigid deformation using the Symmetric Diffeomorphic Registration(SyN) Algorithm:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(static, moving, static_affine, moving_affine,
affine_opt.affine)
warped_moving = mapping.transform(moving)
# We show the registration result with:
regtools.overlay_slices(
static,
warped_moving,
None,
0,
"Static",
"Moving",
f"{working_dir}/transformed_sagittal.png",
)
regtools.overlay_slices(
static,
warped_moving,
None,
1,
"Static",
"Moving",
f"{working_dir}/transformed_coronal.png",
)
regtools.overlay_slices(
static,
warped_moving,
None,
2,
"Static",
"Moving",
f"{working_dir}/transformed_axial.png",
)
return mapping, affine_map
def affine_reg(static_path, moving_path):
"""
:param static_path:
:param moving_path:
:return:
"""
t0_time = time.time()
print('-->Applying affine reg over', basename(moving_path), 'based on',
basename(static_path))
static_img = nib.load(static_path)
static = static_img.get_data()
static_grid2world = static_img.affine
moving_img = nib.load(moving_path)
moving = np.array(moving_img.get_data())
moving_grid2world = moving_img.affine
print('---> I. Translation of the moving image towards the static image')
print(
'---> Resembling the moving image on a grid of the same dimensions as the static image'
)
identity = np.eye(4)
affine_map = AffineMap(identity, static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0, "Static", "Moving",
"resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1, "Static", "Moving",
"resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2, "Static", "Moving",
"resampled_2.png")
print('---> Aligning the centers of mass of the two images')
c_of_mass = transform_centers_of_mass(static, static_grid2world, moving,
moving_grid2world)
print(
'---> We can now transform the moving image and draw it on top of the static image'
)
transformed = c_of_mass.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_com_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_com_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_com_2.png")
print('---> II. Refine by looking for an affine transform')
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10000, 1000, 100]
sigmas = [3.0, 1.0, 0.0]
factors = [4, 2, 1]
"""
Now we go ahead and instantiate the registration class with the configuration
we just prepared
"""
print('---> Computing Affine Registration (non-convex optimization)')
affreg = AffineRegistration(metric=metric,
level_iters=level_iters,
sigmas=sigmas,
factors=factors)
transform = TranslationTransform3D()
params0 = None
starting_affine = c_of_mass.affine
translation = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = translation.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_trans_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_trans_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_trans_2.png")
print('--->III. Refining with a rigid transform')
transform = RigidTransform3D()
params0 = None
starting_affine = translation.affine
rigid = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = rigid.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_rigid_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_rigid_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_rigid_2.png")
print(
'--->IV. Refining with a full afine transform (translation, rotation, scale and shear)'
)
transform = AffineTransform3D()
params0 = None
starting_affine = rigid.affine
affine = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
print('---> Results in a slight shear and scale')
transformed = affine.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_affine_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_affine_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_affine_2.png")
name = os.path.splitext(basename(moving_path))[0] + '_affine_reg'
nib.save(nib.Nifti1Image(transformed, np.eye(4)), name)
t1_time = time.time()
total_time = t1_time - t0_time
print('Total time:' + str(total_time))
return print('Successfully affine registration applied')
def quick_check():
img1_fname = "/home/omar/data/DATA_NeoBrainS12/T1.nii.gz"
img2_fname = "/home/omar/data/DATA_NeoBrainS12/set2_i1_t1.nii.gz"
img1_nib = nib.load(img1_fname)
img1 = img1_nib.get_data().squeeze()
img1_affine = img1_nib.get_affine()
img2_nib = nib.load(img2_fname)
img2 = img2_nib.get_data().squeeze()
img2_affine = img2_nib.get_affine()
# nib.aff2axcodes(img1_affine)
#aff = AffineMap(None, img1.shape, img1_affine, img2.shape, img2_affine)
#aff = transform_centers_of_mass(img1, img1_affine, img2, img2_affine)
aff = dipy_align(img1, img1_affine, img2, img2_affine, np.eye(4))
img2_resampled = aff.transform(img2)
rt.overlay_slices(img1, img2_resampled, slice_type=0)
rt.overlay_slices(img1, img2_resampled, slice_type=1)
rt.overlay_slices(img1, img2_resampled, slice_type=2)
# Verify that original and RAS versions of neo1 describe the same object
# Load original data
neo1_fname = get_neobrain('train', 1, 'T1')
neo1_old, neo1_old_affine, neo1_old_spacing, neo1_old_ori = load_from_raw(
neo1_fname)
# Load RAS version
neo1_nib = nib.load(neo1_fname)
neo1 = neo1_nib.get_data()
neo1_affine = neo1_nib.get_affine()
# Resample RAS on top of original
aff = AffineMap(None, neo1_old.shape, neo1_old_affine, neo1.shape,
neo1_affine)
neo1_resampled = aff.transform(neo1)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=0)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=1)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=2)
# Attempt to resample a test volume on top of training
neo2_fname = get_neobrain('test', 1, 'i1_t1')
neo2_nib = nib.load(neo2_fname)
neo2 = neo2_nib.get_data()
neo2_affine = neo2_nib.get_affine()
aff = transform_centers_of_mass(neo1, neo1_affine, neo2, neo2_affine)
#aff = dipy_align(neo1, neo1_affine, neo2, neo2_affine)
neo2_resampled = aff.transform(neo2)
rt.overlay_slices(neo1, neo2_resampled, slice_type=0)
rt.overlay_slices(neo1, neo2_resampled, slice_type=1)
rt.overlay_slices(neo1, neo2_resampled, slice_type=2)
# Load atlas
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas_affine = atlas_nib.get_affine()
atlas = atlas_nib.get_data()
rt.plot_slices(atlas)
# Resample atlas on top of neo1
aff = AffineMap(None, neo1.shape, neo1_affine, atlas.shape, atlas_affine)
atlas_resampled = aff.transform(atlas)
rt.overlay_slices(neo1, atlas_resampled)
fdwi = "/Volumes/Data/Badea/Lab/mouse/C57_JS/Nikhil_temp/niftifiles/N57437_nii4D_RAS.nii"
fdwi = "/Volumes/Data/Badea/Lab/mouse/C57_JS/Nikhil_temp2/niftifiles/N57437_nii4D_RAS.nii"
#Loading static image
static_data, static_affine, static_img = load_nifti(fdwi, return_img=True)
#static_data, static_affine, static_img = load_nifti(fdwi, return_img=True)
static = np.squeeze(static_data)[..., 0]
static_grid2world = static_affine
#Loading moving image
moving_data, moving_affine, moving_img = load_nifti(bdwi, return_img=True)
moving = moving_data
moving_grid2world = moving_affine
#Visualize images
identity = np.eye(4)
affine_map = AffineMap(identity,
static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0,
"Static", "Moving", "resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1,
"Static", "Moving", "resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2,
"Static", "Moving", "resampled_2.png")
def registration(diff, affine_diff, anat, affine_anat):
#Affine trasformation beetween diffuson and anatomical data
static = np.squeeze(diff)[..., 0]
static_grid2world = affine_diff
moving = anat
moving_grid2world = affine_anat
identity = np.eye(4)
affine_map = AffineMap(identity, static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0, "Static", "Moving",
"resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1, "Static", "Moving",
"resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2, "Static", "Moving",
"resampled_2.png")
c_of_mass = transform_centers_of_mass(static, static_grid2world, moving,
moving_grid2world)
transformed = c_of_mass.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_com_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_com_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_com_2.png")
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10000, 1000, 100]
factors = [4, 2, 1]
sigmas = [3.0, 1.0, 0.0]
affreg = AffineRegistration(metric=metric,
level_iters=level_iters,
sigmas=sigmas,
factors=factors)
transform = TranslationTransform3D()
params0 = None
starting_affine = c_of_mass.affine
translation = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = translation.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_trans_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_trans_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_trans_2.png")
transform = RigidTransform3D()
params0 = None
starting_affine = translation.affine
rigid = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = rigid.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_rigid_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_rigid_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_rigid_2.png")
transform = AffineTransform3D()
params0 = None
starting_affine = rigid.affine
affine = affreg.optimize(static,
moving,
transform,
params0,
static_grid2world,
moving_grid2world,
starting_affine=starting_affine)
transformed = affine.transform(moving)
regtools.overlay_slices(static, transformed, None, 0, "Static",
"Transformed", "transformed_affine_0.png")
regtools.overlay_slices(static, transformed, None, 1, "Static",
"Transformed", "transformed_affine_1.png")
regtools.overlay_slices(static, transformed, None, 2, "Static",
"Transformed", "transformed_affine_2.png")
inverse_map = AffineMap(starting_affine, static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled_inverse = inverse_map.transform_inverse(transformed,
resample_only=True)
nib.save(nib.Nifti1Image(resampled_inverse, affine_diff),
'brain.coreg.nii.gz')
return transformed
def align_atlas():
neo_fname = get_neobrain('train', 1, 'T2')
neo_nib = nib.load(neo_fname)
neo = neo_nib.get_data()
neo_affine = neo_nib.get_affine()
# Load atlas (with skull)
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas = atlas_nib.get_data()
atlas_affine = atlas_nib.get_affine()
# The first training volume dimensions are about 5cm x 5cm x 8cm
# The atlas dimensions are about 7cm x 9cm x 11 cm
# Assuming isotropic scale, the atlas is about 1.5 times larger than
# the input image:
iso_scale = (float(7 * 9 * 11) / float(5 * 5 * 8))**(1.0 / 3)
print(iso_scale)
#We can use this to constraint the transformation to rigid
scale = np.eye(4)
scale[:3, :3] *= iso_scale
rigid_map_fname = 'atlas_towards_neo1_rigid.p'
if os.path.isfile(rigid_map_fname):
rigid_map = pickle.load(open(rigid_map_fname, 'r'))
else:
transforms = ['RIGID']
rigid_map = dipy_align(neo,
neo_affine,
atlas,
atlas_affine,
transforms=transforms,
prealign=scale)
pickle.dump(rigid_map, open(rigid_map_fname, 'w'))
atlas_resampled = rigid_map.transform(atlas)
# Compare anterior coronal slices
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=10,
ltitle='Neo1',
rtitle='Atlas')
# Compare middle coronal slices
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=25,
ltitle='Neo1',
rtitle='Atlas')
# Compare posterior coronal slices
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=40,
ltitle='Neo1',
rtitle='Atlas')
# Load the peeled atlas
atlas_wcerebellum_fname = get_neobrain('atlas', 'neo-withCerebellum', None)
atlas_wcerebellum_nib = nib.load(atlas_wcerebellum_fname)
atlas_wcerebellum = atlas_wcerebellum_nib.get_data()
atlas_wcerebellum_affine = atlas_wcerebellum_nib.get_affine()
# Configure diffeomorphic registration
diff_map_name = 'atlas_towards_neo1_diff.p'
if os.path.isfile(diff_map_name):
diff_map = pickle.load(open(diff_map_name, 'r'))
else:
metric = CCMetric(3)
sdr = SymmetricDiffeomorphicRegistration(metric)
# The atlases are not aligned in physical space!! use atlas_affine instead of atlas_wcerebellum_affine
diff_map = sdr.optimize(neo,
atlas_wcerebellum,
neo_affine,
atlas_affine,
prealign=rigid_map.affine)
pickle.dump(diff_map, open(diff_map_name, 'w'))
atlas_wcerebellum_deformed = diff_map.transform(atlas_wcerebellum)
# Before and after diffeomorphic registration
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=10,
ltitle='Neo1',
rtitle='Atlas')
rt.overlay_slices(neo,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=10,
ltitle='Neo1',
rtitle='Atlas')
# Before and after diffeomorphic registration
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=25,
ltitle='Neo1',
rtitle='Atlas')
rt.overlay_slices(neo,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=25,
ltitle='Neo1',
rtitle='Atlas')
# Before and after diffeomorphic registration
rt.overlay_slices(neo,
atlas_resampled,
slice_type=2,
slice_index=40,
ltitle='Neo1',
rtitle='Atlas')
rt.overlay_slices(neo,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=40,
ltitle='Neo1',
rtitle='Atlas')
# Now all volumes
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas = atlas_nib.get_data()
atlas_affine = atlas_nib.get_affine()
atlas_wcerebellum_fname = get_neobrain('atlas', 'neo-withCerebellum', None)
atlas_wcerebellum_nib = nib.load(atlas_wcerebellum_fname)
atlas_wcerebellum = atlas_wcerebellum_nib.get_data()
atlas_wcerebellum_affine = atlas_wcerebellum_nib.get_affine()
idx = 2
neoi_fname = get_neobrain('train', idx, 'T2')
neoi_nib = nib.load(neoi_fname)
neoi = neoi_nib.get_data()
neoi_affine = neoi_nib.get_affine()
iso_scale = (float(7 * 9 * 11) / float(5 * 5 * 8))**(1.0 / 3)
print(iso_scale)
#We can use this to constraint the transformation to rigid
scale = np.eye(4)
scale[:3, :3] *= iso_scale
rigid_map_fname = 'atlas_towards_neo%d_affine.p' % (idx, )
if os.path.isfile(rigid_map_fname):
rigid_map = pickle.load(open(rigid_map_fname, 'r'))
else:
transforms = ['RIGID', 'AFFINE']
level_iters = [[10000, 1000, 100], [100]]
rigid_map = dipy_align(neoi,
neoi_affine,
atlas,
atlas_affine,
transforms=transforms,
level_iters=level_iters,
prealign=scale)
pickle.dump(rigid_map, open(rigid_map_fname, 'w'))
atlas_resampled = rigid_map.transform(atlas)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=6)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=10)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=25)
rt.overlay_slices(neoi, atlas_resampled, slice_type=2, slice_index=40)
diff_map_name = 'atlas_towards_neo%d_diff.p' % (idx, )
if os.path.isfile(diff_map_name):
diff_map = pickle.load(open(diff_map_name, 'r'))
else:
metric = CCMetric(3)
sdr = SymmetricDiffeomorphicRegistration(metric)
# The atlases are not aligned in physical space!! use atlas_affine instead of atlas_wcerebellum_affine
diff_map = sdr.optimize(neoi,
atlas_wcerebellum,
neoi_affine,
atlas_affine,
prealign=rigid_map.affine)
pickle.dump(diff_map, open(diff_map_name, 'w'))
atlas_wcerebellum_deformed = diff_map.transform(atlas_wcerebellum)
# Before and after diffeomorphic registration
rt.overlay_slices(neoi,
atlas_resampled,
slice_type=2,
slice_index=6,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
rt.overlay_slices(neoi,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=6,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
rt.overlay_slices(neoi,
atlas_resampled,
slice_type=2,
slice_index=10,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
rt.overlay_slices(neoi,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=10,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
# Before and after diffeomorphic registration
rt.overlay_slices(neoi,
atlas_resampled,
slice_type=2,
slice_index=25,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
rt.overlay_slices(neoi,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=25,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
# Before and after diffeomorphic registration
rt.overlay_slices(neoi,
atlas_resampled,
slice_type=2,
slice_index=40,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
rt.overlay_slices(neoi,
atlas_wcerebellum_deformed,
slice_type=2,
slice_index=40,
ltitle='Neo%d' % (idx, ),
rtitle='Atlas')
from dipy.data.fetcher import fetch_syn_data, read_syn_data
from dipy.segment.mask import median_otsu
from dipy.align.transforms import regtransforms
from dipy.align.imaffine import (align_centers_of_mass,
MattesMIMetric,
AffineRegistration)
jit_nib = nib.load('jittered.nii.gz')
jit = jit_nib.get_data().squeeze()
static = jit[...,0]
moving = jit[...,2]
aff_static = jit_nib.get_affine()
aff_moving = jit_nib.get_affine()
rt.overlay_slices(static, moving, slice_type=2)
# Bring the center of mass to the origin
#c_static = ndimage.measurements.center_of_mass(np.array(static))
c_static = tuple(0.5 * np.array(static.shape, dtype=np.float64))
c_static = aff_static.dot(c_static+(1,))
correction_static = np.eye(4, dtype=np.float64)
correction_static[:3,3] = -1 * c_static[:3]
#c_moving = ndimage.measurements.center_of_mass(np.array(moving))
c_moving = tuple(0.5 * np.array(moving.shape, dtype=np.float64))
c_moving = aff_static.dot(c_moving+(1,))
correction_moving = np.eye(4, dtype=np.float64)
correction_moving[:3,3] = -1 * c_moving[:3]
new_aff_static = correction_static.dot(aff_static)
new_aff_moving = correction_moving.dot(aff_moving)
image on the static grid. We create an AffineMap to transform the moving image
towards the static image
"""
from dipy.align.imaffine import AffineMap
affine_map = AffineMap(pre_align,
static.shape, static_affine,
moving.shape, moving_affine)
resampled = affine_map.transform(moving)
"""
plot the overlapped middle slices of the volumes
"""
regtools.overlay_slices(static, resampled, None, 1, 'Static', 'Moving', 'input_3d.png')
"""
.. figure:: input_3d.png
:align: center
**Static image in red on top of the pre-aligned moving image (in green)**.
"""
"""
We want to find an invertible map that transforms the moving image into the
static image. We will use the Cross Correlation metric
"""
metric = CCMetric(3)
brainweb_name = info.get_brainweb("t1", "raw")
brainweb_nib = nib.load(brainweb_name)
brainweb = brainweb_nib.get_data().squeeze()
brainweb_affine = brainweb_nib.get_affine()
brainweb = brainweb.transpose([0, 2, 1])[::-1, :, :]
rt.plot_slices(brainweb)
brainweb_affine = ibsr1_affine.copy()
brainweb_affine[brainweb_affine != 0] = 1
brainweb_affine[0, 0] = -1
# Reslice Brainweb on IBSR1
ibsr_to_bw = AffineMap(None, ibsr1.shape, ibsr1_affine, brainweb.shape, brainweb_affine)
bw_on_ibsr1 = ibsr_to_bw.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1) # misaligned
c_of_mass = transform_centers_of_mass(ibsr1, ibsr1_affine, brainweb, brainweb_affine)
bw_on_ibsr1 = c_of_mass.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1) # roughly aligned
# Start affine alignment
aff_name = "ibsr1_to_brainweb.p"
if os.path.isfile(aff_name):
ibsr_bw_affmap = pickle.load(open(aff_name, "r"))
else:
ibsr_bw_affmap = dipy_align(ibsr1, ibsr1_affine, brainweb, brainweb_affine)
pickle.dump(ibsr_bw_affmap, open(aff_name, "w"))
bw_on_ibsr1 = ibsr_bw_affmap.transform(brainweb)
rt.overlay_slices(ibsr1, bw_on_ibsr1, slice_type=0) # aligned (sagital view)
rt.overlay_slices(ibsr1, bw_on_ibsr1, slice_type=1) # aligned (axial view)
def check_direct(a, b):
static = dwi[..., a]
moving = dwi[..., b]
static_grid2space = dwi_nib.get_affine()
moving_grid2space = dwi_nib.get_affine()
rt.overlay_slices(static, moving, None, 0, "Static", "Warped LCC")
rt.overlay_slices(static, moving, None, 1, "Static", "Warped LCC")
rt.overlay_slices(static, moving, None, 2, "Static", "Warped LCC")
affine_lcc = register("LCC", static, moving, static_grid2space, moving_grid2space)
warped_lcc = transform_image(static, static_grid2space, moving, moving_grid2space, affine_lcc)
rt.overlay_slices(static, warped_lcc, None, 0, "Static", "Warped LCC")
rt.overlay_slices(static, warped_lcc, None, 1, "Static", "Warped LCC")
rt.overlay_slices(static, warped_lcc, None, 2, "Static", "Warped LCC")
return affine_lcc
data = img.get_data()
data_b0=np.squeeze(data)[...,0]
from dipy.data.fetcher import fetch_syn_data, read_syn_data
fetch_syn_data()
nib_syn_t1, nib_syn_b0 = read_syn_data()
syn_b0 = np.array(nib_syn_b0.get_data())
from dipy.segment.mask import median_otsu
data_b0_masked, data_b0_mask = median_otsu(data_b0, 4, 4)
syn_b0_masked, syn_b0_mask = median_otsu(syn_b0, 4, 4)
static = data_b0_masked
static_affine = nib_stanford.get_affine()
moving = syn_b0_masked
moving_affine = nib_syn_b0.get_affine()
pre_align = np.array([[1.02783543e+00, -4.83019053e-02, -6.07735639e-02, -2.57654118e+00],
[4.34051706e-03, 9.41918267e-01, -2.66525861e-01, 3.23579799e+01],
[5.34288908e-02, 2.90262026e-01, 9.80820307e-01, -1.46216651e+01],
[0.00000000e+00, 0.00000000e+00, 0.00000000e+00, 1.00000000e+00]])
import dipy.align.vector_fields as vfu
transform = np.linalg.inv(moving_affine).dot(pre_align.dot(static_affine))
resampled = vfu.warp_3d_affine(moving.astype(np.float32),
np.asarray(static.shape, dtype=np.int32),
transform)
resampled = np.asarray(resampled)
regtools.overlay_slices(static, resampled, None, 1, 'Static', 'Moving', 'input_3d.png')
def register_save(inputpathdir, target_path, subject, outputpath, figspath,
params, registration_types, applydirs, verbose):
anat_path = get_anat(inputpathdir, subject)
#myanat = load_nifti(anat_path)
myanat = nib.load(anat_path)
anat_data = np.squeeze(myanat.get_data()[..., 0])
anat_affine = myanat.affine
anat_hdr = myanat.header
vox_size = myanat.header.get_zooms()[0]
#mynifti = load_nifti("/Volumes/Data/Badea/Lab/19abb14/N57437_nii4D.nii")
#anat_data = np.squeeze(myanat[0])[..., 0]
#anat_affine = myanat[1]
#hdr = myanat.header
mytarget = nib.load(target_path)
target_data = np.squeeze(mytarget.get_data()[..., 0])
target_affine = mytarget.affine
identity = np.eye(4)
affine_map = AffineMap(identity, target_data.shape, target_affine,
anat_data.shape, anat_affine)
resampled = affine_map.transform(anat_data)
"""
regtools.overlay_slices(target_data, resampled, None, 0,
"target_data", "anat_data", figspath + "resampled_0.png")
regtools.overlay_slices(target_data, resampled, None, 1,
"target_data", "anat_data", figspath + "resampled_1.png")
regtools.overlay_slices(target_data, resampled, None, 2,
"target_data", "anat_data", figspath + "resampled_2.png")
"""
c_of_mass = transform_centers_of_mass(target_data, target_affine,
anat_data, anat_affine)
apply_niftis = []
apply_trks = []
if inputpathdir in applydirs:
applyfiles = [anat_path]
else:
applyfiles = []
for applydir in applydirs:
apply_niftis.extend(get_niftis(applydir, subject))
apply_trks.extend(get_trks(applydir, subject))
if "center_mass" in registration_types:
if apply_trks:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(target_data, anat_data, target_affine,
anat_affine, c_of_mass.affine)
for apply_nifti in apply_niftis:
fname = os.path.basename(apply_nifti).split(".")[0]
fpath = outputpath + fname + "_centermass.nii"
applynii = nib.load(apply_nifti)
apply_data = applynii.get_data()
apply_affine = applynii.affine
apply_hdr = myanat.header
if len(np.shape(apply_data)) == 4:
transformed_all = c_of_mass.transform(apply_data, apply4D=True)
transformed = transformed_all[:, :, :, 0]
else:
transformed_all = c_of_mass.transform(apply_data)
transformed = transformed_all
save_nifti(fpath, transformed_all, apply_affine, hdr=apply_hdr)
if figspath is not None:
regtools.overlay_slices(target_data, transformed, None, 0,
"target_data", "Transformed",
figspath + fname + "_centermass_1.png")
regtools.overlay_slices(target_data, transformed, None, 1,
"target_data", "Transformed",
figspath + fname + "_centermass_2.png")
regtools.overlay_slices(target_data, transformed, None, 2,
"target_data", "Transformed",
figspath + fname + "_centermass_3.png")
if verbose:
print("Saved the file at " + fpath)
#mapping = sdr.optimize(target_data, anat_data, target_affine, anat_affine,
# c_of_mass.affine)
#warped_moving = mapping.transform(anat_data)
for apply_trk in apply_trks:
fname = os.path.basename(apply_trk).split(".")[0]
fpath = outputpath + fname + "_centermass.trk"
sft = load_tractogram(apply_trk, 'same')
target_isocenter = np.diag(
np.array([-vox_size, vox_size, vox_size, 1]))
origin_affine = affine_map.affine.copy()
origin_affine[0][3] = -origin_affine[0][3]
origin_affine[1][3] = -origin_affine[1][3]
origin_affine[2][3] = origin_affine[2][3] / vox_size
origin_affine[1][3] = origin_affine[1][3] / vox_size**2
# Apply the deformation and correct for the extents
mni_streamlines = deform_streamlines(
sft.streamlines,
deform_field=mapping.get_forward_field(),
stream_to_current_grid=target_isocenter,
current_grid_to_world=origin_affine,
stream_to_ref_grid=target_isocenter,
ref_grid_to_world=np.eye(4))
if has_fury:
show_template_bundles(mni_streamlines,
anat_data,
show=False,
fname=figspath + fname +
'_streamlines_centermass.png')
sft = StatefulTractogram(mni_streamlines, myanat, Space.RASMM)
save_tractogram(sft, fpath, bbox_valid_check=False)
if verbose:
print("Saved the file at " + fpath)
metric = MutualInformationMetric(params.nbins, params.sampling_prop)
if "AffineRegistration" in registration_types:
affreg = AffineRegistration(metric=metric,
level_iters=params.level_iters,
sigmas=params.sigmas,
factors=params.factors)
transform = TranslationTransform3D()
params0 = None
starting_affine = c_of_mass.affine
translation = affreg.optimize(target_data,
anat_data,
transform,
params0,
target_affine,
anat_affine,
starting_affine=starting_affine)
if apply_trks:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(target_data, anat_data, target_affine,
anat_affine, translation.affine)
for apply_nifti in apply_niftis:
fname = os.path.basename(apply_nifti).split(".")[0]
fpath = outputpath + fname + "_affinereg.nii"
applynii = nib.load(apply_nifti)
apply_data = applynii.get_data()
apply_affine = applynii.affine
apply_hdr = myanat.header
if len(np.shape(apply_data)) == 4:
transformed_all = translation.transform(apply_data,
apply4D=True)
transformed = transformed_all[:, :, :, 0]
else:
transformed_all = translation.transform(apply_data)
transformed = transformed_all
save_nifti(fpath, transformed_all, anat_affine, hdr=anat_hdr)
if figspath is not None:
regtools.overlay_slices(target_data, transformed, None, 0,
"target_data", "Transformed",
figspath + fname + "_affinereg_1.png")
regtools.overlay_slices(target_data, transformed, None, 1,
"target_data", "Transformed",
figspath + fname + "_affinereg_2.png")
regtools.overlay_slices(target_data, transformed, None, 2,
"target_data", "Transformed",
figspath + fname + "_affinereg_3.png")
if verbose:
print("Saved the file at " + fpath)
for apply_trk in apply_trks:
fname = os.path.basename(apply_trk).split(".")[0]
fpath = outputpath + fname + "_affinereg.trk"
sft = load_tractogram(apply_trk, 'same')
target_isocenter = np.diag(
np.array([-vox_size, vox_size, vox_size, 1]))
origin_affine = affine_map.affine.copy()
origin_affine[0][3] = -origin_affine[0][3]
origin_affine[1][3] = -origin_affine[1][3]
origin_affine[2][3] = origin_affine[2][3] / vox_size
origin_affine[1][3] = origin_affine[1][3] / vox_size**2
# Apply the deformation and correct for the extents
mni_streamlines = deform_streamlines(
sft.streamlines,
deform_field=mapping.get_forward_field(),
stream_to_current_grid=target_isocenter,
current_grid_to_world=origin_affine,
stream_to_ref_grid=target_isocenter,
ref_grid_to_world=np.eye(4))
if has_fury:
show_template_bundles(mni_streamlines,
anat_data,
show=False,
fname=figspath + fname +
'_streamlines_affinereg.png')
sft = StatefulTractogram(mni_streamlines, myanat, Space.RASMM)
save_tractogram(sft, fpath, bbox_valid_check=False)
if verbose:
print("Saved the file at " + fpath)
if "RigidTransform3D" in registration_types:
transform = RigidTransform3D()
params0 = None
if 'translation' not in locals():
affreg = AffineRegistration(metric=metric,
level_iters=params.level_iters,
sigmas=params.sigmas,
factors=params.factors)
translation = affreg.optimize(target_data,
anat_data,
transform,
params0,
target_affine,
anat_affine,
starting_affine=c_of_mass.affine)
starting_affine = translation.affine
rigid = affreg.optimize(target_data,
anat_data,
transform,
params0,
target_affine,
anat_affine,
starting_affine=starting_affine)
transformed = rigid.transform(anat_data)
if apply_trks:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(target_data, anat_data, target_affine,
anat_affine, rigid.affine)
for apply_nifti in apply_niftis:
fname = os.path.basename(apply_nifti).split(".")[0]
fpath = outputpath + fname + "_rigidtransf3d.nii"
applynii = nib.load(apply_nifti)
apply_data = applynii.get_data()
apply_affine = applynii.affine
apply_hdr = myanat.header
if len(np.shape(apply_data)) == 4:
transformed_all = rigid.transform(apply_data, apply4D=True)
transformed = transformed_all[:, :, :, 0]
else:
transformed_all = rigid.transform(apply_data)
transformed = transformed_all
save_nifti(fpath, transformed_all, anat_affine, hdr=anat_hdr)
if figspath is not None:
regtools.overlay_slices(
target_data, transformed, None, 0, "target_data",
"Transformed", figspath + fname + "_rigidtransf3d_1.png")
regtools.overlay_slices(
target_data, transformed, None, 1, "target_data",
"Transformed", figspath + fname + "_rigidtransf3d_2.png")
regtools.overlay_slices(
target_data, transformed, None, 2, "target_data",
"Transformed", figspath + fname + "_rigidtransf3d_3.png")
if verbose:
print("Saved the file at " + fpath)
for apply_trk in apply_trks:
fname = os.path.basename(apply_trk).split(".")[0]
fpath = outputpath + fname + "_rigidtransf3d.trk"
sft = load_tractogram(apply_trk, 'same')
target_isocenter = np.diag(
np.array([-vox_size, vox_size, vox_size, 1]))
origin_affine = affine_map.affine.copy()
origin_affine[0][3] = -origin_affine[0][3]
origin_affine[1][3] = -origin_affine[1][3]
origin_affine[2][3] = origin_affine[2][3] / vox_size
origin_affine[1][3] = origin_affine[1][3] / vox_size**2
# Apply the deformation and correct for the extents
mni_streamlines = deform_streamlines(
sft.streamlines,
deform_field=mapping.get_forward_field(),
stream_to_current_grid=target_isocenter,
current_grid_to_world=origin_affine,
stream_to_ref_grid=target_isocenter,
ref_grid_to_world=np.eye(4))
if has_fury:
show_template_bundles(mni_streamlines,
anat_data,
show=False,
fname=figspath + fname +
'_rigidtransf3d.png')
sft = StatefulTractogram(mni_streamlines, myanat, Space.RASMM)
save_tractogram(sft, fpath, bbox_valid_check=False)
if verbose:
print("Saved the file at " + fpath)
if "AffineTransform3D" in registration_types:
transform = AffineTransform3D()
params0 = None
starting_affine = rigid.affine
affine = affreg.optimize(target_data,
anat_data,
transform,
params0,
target_affine,
anat_affine,
starting_affine=starting_affine)
transformed = affine.transform(anat_data)
if apply_trks:
metric = CCMetric(3)
level_iters = [10, 10, 5]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
mapping = sdr.optimize(target_data, anat_data, target_affine,
anat_affine, affine.affine)
for apply_nifti in apply_niftis:
fname = os.path.basename(apply_nifti).split(".")[0]
fpath = outputpath + fname + "_affinetransf3d.nii"
applynii = nib.load(apply_nifti)
apply_data = applynii.get_data()
apply_affine = applynii.affine
apply_hdr = myanat.header
if len(np.shape(apply_data)) == 4:
transformed_all = affine.transform(apply_data, apply4D=True)
transformed = transformed_all[:, :, :, 0]
else:
transformed_all = affine.transform(apply_data)
transformed = transformed_all
save_nifti(fpath, transformed_all, anat_affine, hdr=anat_hdr)
if figspath is not None:
regtools.overlay_slices(
target_data, transformed, None, 0, "target_data",
"Transformed", figspath + fname + "_affinetransf3d_1.png")
regtools.overlay_slices(
target_data, transformed, None, 1, "target_data",
"Transformed", figspath + fname + "_affinetransf3d_2.png")
regtools.overlay_slices(
target_data, transformed, None, 2, "target_data",
"Transformed", figspath + fname + "_affinetransf3d_3.png")
if verbose:
print("Saved the file at " + fpath)
for apply_trk in apply_trks:
fname = os.path.basename(apply_trk).split(".")[0]
fpath = outputpath + fname + "_affinetransf3d.trk"
sft = load_tractogram(apply_trk, 'same')
target_isocenter = np.diag(
np.array([-vox_size, vox_size, vox_size, 1]))
origin_affine = affine_map.affine.copy()
origin_affine[0][3] = -origin_affine[0][3]
origin_affine[1][3] = -origin_affine[1][3]
origin_affine[2][3] = origin_affine[2][3] / vox_size
origin_affine[1][3] = origin_affine[1][3] / vox_size**2
# Apply the deformation and correct for the extents
mni_streamlines = deform_streamlines(
sft.streamlines,
deform_field=mapping.get_forward_field(),
stream_to_current_grid=target_isocenter,
current_grid_to_world=origin_affine,
stream_to_ref_grid=target_isocenter,
ref_grid_to_world=np.eye(4))
if has_fury:
show_template_bundles(mni_streamlines,
anat_data,
show=False,
fname=figspath + fname +
'_streamlines_affinetransf3d.png')
sft = StatefulTractogram(mni_streamlines, myanat, Space.RASMM)
save_tractogram(sft, fpath, bbox_valid_check=False)
if verbose:
print("Saved the file at " + fpath)
volumes by overlapping them over two channels of a color image. To do that we
need them to be sampled on the same grid, so let's first re-sample the moving
image on the static grid. We create an AffineMap to transform the moving image
towards the static image
"""
from dipy.align.imaffine import AffineMap
affine_map = AffineMap(pre_align, static.shape, static_affine, moving.shape,
moving_affine)
resampled = affine_map.transform(moving)
"""
plot the overlapped middle slices of the volumes
"""
regtools.overlay_slices(static, resampled, None, 1, 'Static', 'Moving',
'input_3d.png')
"""
.. figure:: input_3d.png
:align: center
Static image in red on top of the pre-aligned moving image (in green).
"""
"""
We want to find an invertible map that transforms the moving image into the
static image. We will use the Cross Correlation metric
"""
metric = CCMetric(3)
"""
Now we define an instance of the registration class. The SyN algorithm uses
a multi-resolution approach by building a Gaussian Pyramid. We instruct the
def generate_transformed_images(static_filename,
moving_filename,
SCALE,
affines_dir,
output_dir,
subset='both'):
"""
applies affines_dir/*.txt affines to moving image
saves transformed *.nii.gz and middle slice overlays *.png in output_dir
**MUST USE SAME ARGS AS `affine_registration`
Parameters
----------
static_filename : str
path to static file
moving_filename : str
path to moving file
SCALE : float
>0, rescale 3D array axes by SCALE factor (useful to downsample images for faster registration)
affines_dir : str
path to dir where affines are saved as *.txt
output_dir : str
path to dir where transformed *.nii.gz and *.png are saved
subset : str
'png' = only png overlays
'nii' = only nii files
'both' = png and nii files
Returns
-------
None
"""
# extract affine file name from moving_filename
affine_prefix = moving_filename.split('/')[-1]
idx = affine_prefix.find('.nii')
affine_prefix = affine_prefix[:idx]
# summary file, save info about how images are generate
summary_file = open(
pjoin(output_dir, affine_prefix + '_generated_images_summary.csv'),
'w')
summary_wr = csv.writer(summary_file)
summary_wr.writerow([
'static_filename', 'moving_filename', 'transform_affine',
'negative_MI', 'generated_nii', 'generated_png'
])
# load static and moving images, downsample
static, static_affine = rescale_img(static_filename, SCALE)
moving, moving_affine = rescale_img(moving_filename, SCALE)
affine_txt = [
i for i in os.listdir(affines_dir)
if i.find(affine_prefix) > -1 and i[-4:] == '.txt'
]
for affine_filename in affine_txt:
print('... applying ' + affine_filename)
# get prefix for output files
idx = affine_filename.find('.txt')
prefix = affine_filename[:idx]
# get affine values
current_affine = load_affine(affines_dir, affine_filename)
# resample moving image
updated_moving_affine = current_affine.dot(moving_affine)
resampled = resample(static, moving, static_affine,
updated_moving_affine)
# calculate neg_MI
neg_MI = neg_mutual_info(static, resampled)
# save resampled *.nii.gz images with static_affine
nii_name = None
if subset in ['nii', 'both']:
nii_name = pjoin(output_dir, prefix + '.nii.gz')
img = nib.Nifti1Image(resampled, static_affine)
nib.save(img, nii_name)
# save slice overlays with regtools from dipy.viz
png_names = None
if subset in ['png', 'both']:
png_names = [None] * 3
for i in range(3):
png_names[i] = pjoin(output_dir,
prefix + '_' + str(i) + '.png')
regtools.overlay_slices(static, resampled, None, i, 'Static',
'Moving', png_names[i])
plt.close('all')
record = [
static_filename, moving_filename, affine_filename, neg_MI,
nii_name, png_names
]
summary_wr.writerow(record)
summary_file.close()
"""
affmat = np.eye(4)
affmat[0, -1] = 4
affmat[1, -1] = 12
theta = 0.1
c, s = np.cos(theta), np.sin(theta)
affmat[0:2, 0:2] = np.array([[c, -s], [s, c]])
affine_map = AffineMap(affmat, static.shape, static_grid2world, static.shape,
static_grid2world)
moving = affine_map.transform(static)
moving_affine = static_affine.copy()
moving_grid2world = static_grid2world.copy()
regtools.overlay_slices(static, moving, None, 2, "Static", "Moving",
"deregistered.png")
"""
.. figure:: deregistered.png
:align: center
Same images but misaligned.
"""
"""
Let's make some registration settings.
"""
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
# small number of iterations for this example
moving = moving_resized
moving_affine = SCALE_affine.dot(moving_affine)
nbins = 32
sampling_prop = None
metric = MutualInformationMetric(nbins, sampling_prop)
level_iters = [10000, 1000, 100]
sigmas = [3.0, 1.0, 0.0]
factors = [4, 2, 1]
resampled = basic_resample(static, moving, static_affine, moving_affine)
regtools.overlay_slices(static, resampled, None, 0, "Static", "Moving",
"resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1, "Static", "Moving",
"resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2, "Static", "Moving",
"resampled_2.png")
cmass = com_transform(static, moving, static_affine, moving_affine)
cmass_image = cmass.transform(moving)
regtools.overlay_slices(static, cmass_image, None, 0, "Static", "Moving",
"cmass_0.png")
regtools.overlay_slices(static, cmass_image, None, 1, "Static", "Moving",
"cmass_1.png")
regtools.overlay_slices(static, cmass_image, None, 2, "Static", "Moving",
"cmass_2.png")
moving_grid2world = nib_syn_b0.affine
"""
We can see that the images are far from aligned by drawing one on top of
the other. The images don't even have the same number of voxels, so in order
to draw one on top of the other we need to resample the moving image on a grid
of the same dimensions as the static image, we can do this by "transforming"
the moving image using an identity transform
"""
identity = np.eye(4)
affine_map = AffineMap(identity,
static.shape, static_grid2world,
moving.shape, moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0,
"Static", "Moving", "resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1,
"Static", "Moving", "resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2,
"Static", "Moving", "resampled_2.png")
"""
.. figure:: resampled_0.png
:align: center
.. figure:: resampled_1.png
:align: center
.. figure:: resampled_2.png
:align: center
Input images before alignment.
"""
def quick_check():
img1_fname = "/home/omar/data/DATA_NeoBrainS12/T1.nii.gz"
img2_fname = "/home/omar/data/DATA_NeoBrainS12/set2_i1_t1.nii.gz"
img1_nib = nib.load(img1_fname)
img1 = img1_nib.get_data().squeeze()
img1_affine = img1_nib.get_affine()
img2_nib = nib.load(img2_fname)
img2 = img2_nib.get_data().squeeze()
img2_affine = img2_nib.get_affine()
# nib.aff2axcodes(img1_affine)
#aff = AffineMap(None, img1.shape, img1_affine, img2.shape, img2_affine)
#aff = transform_centers_of_mass(img1, img1_affine, img2, img2_affine)
aff = dipy_align(img1, img1_affine, img2, img2_affine, np.eye(4))
img2_resampled = aff.transform(img2)
rt.overlay_slices(img1, img2_resampled, slice_type=0)
rt.overlay_slices(img1, img2_resampled, slice_type=1)
rt.overlay_slices(img1, img2_resampled, slice_type=2)
# Verify that original and RAS versions of neo1 describe the same object
# Load original data
neo1_fname = get_neobrain('train', 1, 'T1')
neo1_old, neo1_old_affine, neo1_old_spacing, neo1_old_ori = load_from_raw(neo1_fname)
# Load RAS version
neo1_nib = nib.load(neo1_fname)
neo1 = neo1_nib.get_data()
neo1_affine = neo1_nib.get_affine()
# Resample RAS on top of original
aff = AffineMap(None, neo1_old.shape, neo1_old_affine, neo1.shape, neo1_affine)
neo1_resampled = aff.transform(neo1)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=0)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=1)
rt.overlay_slices(neo1_old, neo1_resampled, slice_type=2)
# Attempt to resample a test volume on top of training
neo2_fname = get_neobrain('test', 1, 'i1_t1')
neo2_nib = nib.load(neo2_fname)
neo2 = neo2_nib.get_data()
neo2_affine = neo2_nib.get_affine()
aff = transform_centers_of_mass(neo1, neo1_affine, neo2, neo2_affine)
#aff = dipy_align(neo1, neo1_affine, neo2, neo2_affine)
neo2_resampled = aff.transform(neo2)
rt.overlay_slices(neo1, neo2_resampled, slice_type=0)
rt.overlay_slices(neo1, neo2_resampled, slice_type=1)
rt.overlay_slices(neo1, neo2_resampled, slice_type=2)
# Load atlas
atlas_fname = get_neobrain('atlas', 'neo-withSkull', None)
atlas_nib = nib.load(atlas_fname)
atlas_affine = atlas_nib.get_affine()
atlas = atlas_nib.get_data()
rt.plot_slices(atlas)
# Resample atlas on top of neo1
aff = AffineMap(None, neo1.shape, neo1_affine, atlas.shape, atlas_affine)
atlas_resampled = aff.transform(atlas)
rt.overlay_slices(neo1, atlas_resampled)
moving_data, moving_affine = load_nifti(pjoin(folder, 'b0.nii.gz'))
moving = moving_data
moving_grid2world = moving_affine
"""
We can see that the images are far from aligned by drawing one on top of
the other. The images don't even have the same number of voxels, so in order
to draw one on top of the other we need to resample the moving image on a grid
of the same dimensions as the static image, we can do this by "transforming"
the moving image using an identity transform
"""
identity = np.eye(4)
affine_map = AffineMap(identity, static.shape, static_grid2world, moving.shape,
moving_grid2world)
resampled = affine_map.transform(moving)
regtools.overlay_slices(static, resampled, None, 0, "Static", "Moving",
"resampled_0.png")
regtools.overlay_slices(static, resampled, None, 1, "Static", "Moving",
"resampled_1.png")
regtools.overlay_slices(static, resampled, None, 2, "Static", "Moving",
"resampled_2.png")
"""
.. figure:: resampled_0.png
:align: center
.. figure:: resampled_1.png
:align: center
.. figure:: resampled_2.png
:align: center
Input images before alignment.
"""
"""
"""
from dipy.io.image import load_nifti
mynifti = load_nifti("/Volumes/Data/Badea/Lab/19abb14/N57437_nii4D.nii")
nifti_data = np.squeeze(mynifti[0])[..., 0]
nifti_affine = mynifti[1]
mynifti = load_nifti("/Volumes/Data/Badea/Lab/19abb14/N57500_nii4D.nii")
nifti_data2 = np.squeeze(mynifti[0])[..., 0]
nifti_affine2 = mynifti[1]
identity = np.eye(4)
affine_map = AffineMap(identity, nifti_data.shape, nifti_affine,
nifti_data2.shape, nifti_affine2)
resampled = affine_map.transform(nifti_data2)
regtools.overlay_slices(nifti_data, resampled, None, 0, "nifti_data",
"nifti_data2", "resampled_0.png")
"""
#regtools.overlay_slices(nifti_data, nifti_data2, None, 0,
# "nifti_data", "nifti_data2", "resampled_0.png")
#regtools.overlay_slices(nifti_data, resampled, None, 1,
# "nifti_data", "nifti_data2", "resampled_1.png")
#regtools.overlay_slices(nifti_data, resampled, None, 2,
"nifti_data", "nifti_data2", "resampled_2.png")
"""
c_of_mass = transform_centers_of_mass(nifti_data, nifti_affine, nifti_data2,
nifti_affine2)
transformed = c_of_mass.transform(nifti_data2)
regtools.overlay_slices(nifti_data, transformed, None, 0, "nifti_data",
"Transformed", "transformed_com_0.png")
