def prinseq_task(opc, out_dir, input_1, input_2, basename, opts, tasks):
''' Defines prinseq task. Uses GEN_PATH_DIR(), PATH_PRINSEQ
Params :
input_1 - a list of 1/left fastq files
input_2 - a list of 2/right fastq files
basename - the basename for all output files
opts - optional params for trinity task.
tasks = the tasks that this task is dependent on
'''
trgs = [
'{0!s}/{1!s}_1_{2!s}'.format(out_dir, basename,
os.path.basename(input_1)),
'{0!s}/{1!s}_2_{2!s}'.format(out_dir, basename,
os.path.basename(input_2))
]
pseudo_trgs = [
'{0!s}/{1!s}_{2!s}.fastq'.format(out_dir, basename, x)
for x in range(1, 3)
]
cmd = (
'perl {0!s} -fastq {1!s} -fastq2 {2!s} --out_format 3 --out_good {3!s}/{4!s} '
'--out_bad null --trim_qual_left 20 --trim_qual_right 20 --trim_qual_type min '
'--min_len 55 --trim_tail_left 8 --trim_tail_right 8 {5!s} -log; mv {6!s} {7!s};'
' mv {8!s} {9!s};').format(
tool_path_check(TOOLS_DICT['prinseq'].full_exe[0]), input_1,
input_2, out_dir, basename, opts, pseudo_trgs[0], trgs[0],
pseudo_trgs[1], trgs[1])
name = basename
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
name=name,
stdout=out,
stderr=err,
targets=trgs)
def busco_task(opc, dbs, assembly_path, assembly_name, out_dir, reference_name,
cpu_cap, tasks):
''' Defines the busco task. Uses PATH_DIR, PATH_BUSCO, PATH_BUSCO_REFERENCE
Params :
reference_name - Name of the reference file to be used by busco
cpu_cap - the cpu limit to be gicen to busco.
tasks - a list of tasks that this task is dependant on.
'''
trgs = [
'{0!s}/run_busco_{1!s}_{2!s}'.format(out_dir, assembly_name,
reference_name)
]
busco_db = dbs['busco_' + reference_name]
cmd = ('cd {0!s}; python {1!s} '
'-o busco_{3!s}_{2!s} -i {4!s} -l {5!s}/{2!s}_odb9 -m '
'tran -f -c {6!s}').format(
out_dir, tool_path_check(TOOLS_DICT['busco'].full_exe[0]),
reference_name, assembly_name, assembly_path,
busco_db.call_path, cpu_cap)
name = 'busco_' + reference_name + '_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
cpu=cpu_cap,
stdout=out,
stderr=err)
def trinity_task(opc, path_assembly, out_dir, fastq, fastq2, unpaired,
cpu_cap_trin, cpu_cap_bfly, mem_trin, mem_bfly,
normalize_flag, tasks):
''' Defines the trinity task. Uses GEN_PATH_DIR(), PATH_TRINITY, NAME_ASSEMBLY
Params :
left - a 1/left fastq files
right - a 2/right fastq files
cpu_cap - number of threads used by trinity
tasks - a list of tasks that this task is dependent on
'''
normalize_flag = '--normalize_reads' if (normalize_flag) else ''
input_str = ''
if (unpaired != [] and fastq == []):
input_str += '--single ' + ','.join(unpaired)
if (fastq != []):
input_str += '--left ' + ','.join(fastq + unpaired)
input_str += ' --right ' + ','.join(fastq2)
trgs = [path_assembly]
cmd = (
'{0!s} --seqType fq {1!s} --CPU {2!s} --max_memory {3!s}G --bflyCalculateCPU {4!s} '
'--output {6!s}/trinity; cp {6!s}/trinity/Trinity.fasta {7!s};'
).format(tool_path_check(TOOLS_DICT['trinity'].full_exe[0]), input_str,
cpu_cap_trin, mem_trin, normalize_flag, mem_bfly, out_dir,
trgs[0])
name = 'trinity_assembly'
out, err = gen_logs(opc.path_logs, name)
cpu_cap = max(cpu_cap_trin, cpu_cap_bfly)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
cpu=cpu_cap,
stdout=out,
stderr=err)
def filter_task(assembly_path,
assembly_name,
out_dir,
quant_file_list,
tpm_threshold,
tpm_column_index,
tasks,
log_flag=True,
opc=None):
# TPM column index: transrate uses older salmon; use index =2. Newer salmon: index=3
trgs = [
'{0!s}/{1!s}_{2!s}tpm.fasta'.format(out_dir, assembly_name,
tpm_threshold)
]
quants = ''.join(' --quant_files ' + x for x in quant_file_list)
cmd = (
'python {0!s}/filter_contigs_by_tpm.py --assembly {1!s} --tpm {2!s} {3!s} '
'--out {4!s} --tpm_column_index {5!s}').format(statics.PATH_UTIL,
assembly_path,
tpm_threshold, quants,
trgs[0],
tpm_column_index)
name = 'filt_{0!s}_{1!s}tpm'.format(assembly_name, tpm_threshold)
out, err = gen_logs(opc.path_logs, name) if (log_flag) else (None, None)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def salmon_task(opc, index, left, right, out_name, gene_map, out_dir, cpu_cap,
tasks):
trgs = [
'{0!s}/{1!s}/quant.sf'.format(out_dir, out_name),
'{0!s}/{1!s}/quant.genes.sf'.format(out_dir, out_name)
]
if len(gene_map) > 0:
trans_gene_map = ' --geneMap {0!s}'.format(gene_map)
cmd = (
'{0!s} quant -i {1!s} -l IU -1 {2!s} -2 {3!s} -o {4!s}/{5!s} '
#'--geneMap {6!s} -p {7!s} --extraSensitive; cp {4!s}/{5!s}/quant.sf '
'{6!s} -p {7!s} --dumpEq'
).format(
#cmd = '{0!s} quant -i {1!s} -l IU -1 {2!s} -2 {3!s} -o {4!s}/{5!s}
#--geneMap {6!s} -p {7!s} --extraSensitive --numBootstraps 30 --biasCorrect ; cp ' \
tool_path_check(TOOLS_DICT['salmon'].full_exe[0]),
index,
left,
right,
out_dir,
out_name,
trans_gene_map,
cpu_cap)
name = os.path.basename(index) + '_' + os.path.basename(left)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err,
cpu=cpu_cap)
def salmon_unpaired_task(opc, index, unpaired, out_name, gene_map, out_dir,
cpu_cap, tasks):
trgs = [
'{0!s}/{1!s}_quant.sf'.format(out_dir, out_name),
'{0!s}/{1!s}_quant.genes.sf'.format(out_dir, out_name)
]
if len(gene_map) > 0:
trans_gene_map = ' --geneMap {0!s}'.format(gene_map)
cmd = (
'{0!s} quant -i {1!s} -l U -r {2!s} -o {3!s}/{4!s} {5!s} '
#'-p {6!s} --extraSensitive; cp {3!s}/{4!s}/quant.sf '
'-p {6!s} --dumpEq --extraSensitive; cp {3!s}/{4!s}/quant.sf '
'{3!s}/{4!s}_quant.sf; cp {3!s}/{4!s}/quant.genes.sf '
'{3!s}/{4!s}_quant.genes.sf').format(
tool_path_check(TOOLS_DICT['salmon'].full_exe[0]), index, unpaired,
out_dir, out_name, trans_gene_map, cpu_cap)
name = 'salmon_unpaired_' + os.path.basename(
index) + '_' + os.path.basename(unpaired)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err,
cpu=cpu_cap)
def trimmomatic_task(opc, out_dir, left, right, cpu_cap, basename, tasks):
base_str = '{0!s}/{1!s}'.format(out_dir, basename)
trgs = [
base_str + '_1_' + os.path.basename(left),
base_str + '_2_' + os.path.basename(right)
]
orphans = [
base_str + '_1s_' + os.path.basename(left),
base_str + '_2s_' + os.path.basename(right)
]
cmd = ('java -jar {0!s} PE -threads {3!s} {1!s} {2!s} {5!s} {4!s} {7!s} '
'{6!s} ILLUMINACLIP:{8!s}:2:30:10 LEADING:3 TRAILING:3 '
'SLIDINGWINDOW:4:15 MINLEN:35').format(
tool_path_check(TOOLS_DICT['trimmomatic'].full_exe[0]), left,
right, cpu_cap, orphans[0], trgs[0], orphans[1], trgs[1],
TOOLS_DICT['trimmomatic'].full_exe[1])
name = basename
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
name=name,
stdout=out,
stderr=err,
targets=trgs,
cpu=cpu_cap)
def signalp_task(opc, path_orfs, out_dir, tasks):
out_name = os.path.basename(path_orfs).split('.')[0]
trgs = ['{0!s}/{1!s}.signalp'.format(out_dir, out_name)]
cmd = '{0!s} -f short -n {1!s} {2!s}'.format(tool_path_check(TOOLS_DICT['signalp'].full_exe[0]), trgs[-1], path_orfs)
name = 'signalp_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def blast_augment_task(opc, db, blast, tasks):
id2name = db+'.stitle'
trgs = ['{0!s}_ex'.format(blast)]
cmd = 'python {0!s}/addStitleToBlastTab.py --db2Name {1!s} --blast {2!s} > {3!s}'.format(
statics.PATH_UTIL, id2name, blast, trgs[0])
name = 'Blast_Augmentation_'+os.path.basename(blast)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def assembly_to_bed_task(opc, path_assembly, out_dir, tasks):
assembly_name = os.path.basename(path_assembly).split('.fa')[0]
trgs = ['{0!s}/{1!s}.bed'.format(out_dir, assembly_name)]
cmd = 'python {0!s}/fasta_to_bed_count_length.py {1!s} {2!s}'.format(
statics.PATH_UTIL, path_assembly, trgs[0])
name = 'fasta_to_bed_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def pipeplot_task(opc, dbs, annotation_table, out_dir, tasks):
trgs = ['{0!s}/plots/cogMultiple.png'.format(out_dir)]
# pipeplot no targets
cmd = 'mkdir -p {0!s}/plots ; cd {0!s}/plots ; python {1!s}/pipePlot.py -i {2!s} --nog_categories {3!s};'.format(
out_dir, statics.PATH_UTIL, annotation_table, dbs['nog_categories'].call_path)
name = 'pipeplot'
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def gff3_task(opc, path_assembly, out_path, opts, tasks):
trgs = [out_path]
cmd = ('python {0!s}/annot_table_gff3.py --fasta {1!s} --outfile {2!s} '
).format(statics.PATH_UTIL, path_assembly, out_path)
cmd += ' '.join(['--'+k+' '+opts[k] for k in opts])
name = 'build_gff3_' + os.path.basename(out_path)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def pfam_seq_task(opc, dbs, path_orfs, out_dir, cpu_cap, tasks):
out_name = os.path.basename(path_orfs).split('.')[0]
trgs = ['{0!s}/{1!s}.pfam_tblout'.format(out_dir, out_name)]
cmd = '{0!s} --cpu {1!s} --tblout {2!s} {3!s} {4!s}'.format(
tool_path_check(TOOLS_DICT['hmmer'].full_exe[0]), cpu_cap,
trgs[0], dbs['pfam'].call_path, path_orfs)
name = 'pfam_tblout_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err, cpu=cpu_cap)
def gene_trans_map_task(opc, path_assembly, out_dir, tasks):
assembly_name = os.path.basename(path_assembly).split('.fa')[0]
trgs = ['{0!s}/{1!s}.gene_trans_map'.format(out_dir, assembly_name)]
cmd = 'perl {0!s} {1!s} > {2!s}'.format(
tool_path_check(TOOLS_DICT['trinity'].full_exe[1]),
path_assembly, trgs[0])
name = 'gene_trans_map_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def tmhmm_task(opc, path_orfs, out_dir, tasks):
out_name = os.path.basename(path_orfs).split('.')[0]
trgs = ['{0!s}/{1!s}.tmhmm'.format(out_dir, out_name)]
cmd = 'cd {0!s}; {1!s} --short < {2!s} > {3!s}'.format(
out_dir, tool_path_check(TOOLS_DICT['tmhmm'].full_exe[0]),
path_orfs, trgs[0])
name = 'tmhmm_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def kegg_task(opc, annotation_table, out_dir, tasks, kegg_map_id='ko01100'):
kegg_dir = '{0!s}/kegg_maps'.format(out_dir)
trgs = ['{0!s}/{1!s}.pdf'.format(kegg_dir, kegg_map_id),
'{0!s}/{1!s}_KO.txt'.format(kegg_dir, kegg_map_id)]
cmd = ('mkdir -p {3!s} ; cd {3!s} ; python {0!s}/color_pathways2.py --path {1!s} '
' --transcriptomeKO {2!s} --output {3!s}').format(
statics.PATH_UTIL, kegg_map_id, annotation_table, kegg_dir)
name = 'draw_kegg_map_{0!s}_{1!s}'.format(os.path.basename(annotation_table), kegg_map_id)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def rnammer_task(opc, path_assembly, out_dir, tasks):
assembly_name = os.path.basename(path_assembly).split('.fa')[0]
path_to_rnammer = os.path.dirname(TOOLS_DICT['rnammer'].folder_name)
trgs = ['{0!s}/{1!s}.fasta.rnammer.gff'.format(out_dir, assembly_name)]
cmd = ("cd {0!s}; {1!s} --transcriptome {2!s} --path_to_rnammer {4!s} "
"--org_type euk; cd -").format(
out_dir, tool_path_check(TOOLS_DICT['rnammer'].full_exe[0]),
path_assembly, path_to_rnammer)
name = 'rnammer_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err)
def transdecoder_longorfs_task(opc, path_assembly, path_transdecoder_output, cpu_cap, tasks):
assembly_name = os.path.basename(path_assembly).split('.fa')[0]
longorf_outbase = os.path.join(path_transdecoder_output, opc.assembly_name + '.fasta.transdecoder_dir')
trgs = ['{0!s}/longest_orfs.pep'.format(longorf_outbase),
'{0!s}/longest_orfs.gff3'.format(longorf_outbase),
'{0!s}/longest_orfs.cds'.format(longorf_outbase)]
cmd = ("mkdir -p {0!s}; cd {0!s}; {1!s} -t {2!s}").format(path_transdecoder_output,
tool_path_check(TOOLS_DICT['transdecoder'].full_exe[0]), path_assembly, cpu_cap)
name = 'TransDecoder_LongORFs_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, targets=trgs, name=name, stdout=out, stderr=err, cpu=cpu_cap)
def sam_sort_task(opc, out_dir, bam_file, out_name, tasks):
trgs = ['{0!s}/{1!s}.bam'.format(out_dir, out_name)]
cmd = 'samtools sort {0!s} {1!s}/{2!s}'.format(bam_file, out_dir, out_name)
name = 'sam_sort_' + os.path.basename(bam_file) + '_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def salmon_gene_map_task(opc, out_dir, assembly_name, gene_trans_map, tasks):
''' salmon requires gene_trans_map in reverse column order (transcript \\t gene \\n)'''
trgs = ['{0!s}/{1!s}.trans_gene_map'.format(out_dir, assembly_name)]
cmd = '''awk '{{ print $2 " " $1}}' {0!s} > {1!s}'''.format(
gene_trans_map, trgs[0])
name = 'salmon_gene_map_task_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def build_bowtie_task(opc, path_assembly, assembly_name, out_dir, tasks):
trgs = ['{0!s}/{1!s}.1.bt2'.format(out_dir, assembly_name)]
cmd = '{0!s} --offrate 1 -f {1!s} {2!s}/{3!s}'.format(
tool_path_check(TOOLS_DICT['bowtie2'].full_exe[0]), path_assembly,
out_dir, assembly_name)
name = 'build_bowtie_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def diamond_task(opc, blast_type, out_dir, path_query, ref, cpu_cap, tasks):
''' valid blast_types: "blastx", "blastp" '''
base_ref = os.path.basename(ref)
query_name = os.path.basename(path_query).split('.')[0]
trgs = ['{0!s}/{1!s}_{2!s}.diamond_{3!s}'.format(out_dir, query_name, base_ref, blast_type)]
pseudo_trgs = ['{0!s}/diamond_{1!s}_{2!s}'.format(out_dir, base_ref, blast_type)]
cmd = ('{0!s} {1!s} --db {2!s} --query {3!s} --daa {4!s} --tmpdir {5!s} '
'--max-target-seqs 20 --sensitive --threads {6!s} --evalue 0.001; {0!s} view '
'--daa {4!s}.daa --out {7!s};').format(
tool_path_check(TOOLS_DICT['diamond'].full_exe[0]), blast_type, ref, path_query,
pseudo_trgs[0], out_dir, cpu_cap, trgs[0])
name = 'diamond_{0!s}_{1!s}_{2!s}'.format(blast_type, base_ref, query_name)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd, dependencies=tasks, cpu=cpu_cap, targets=trgs, name=name, stdout=out, stderr=err)
def kallisto_task(opc, index, out_dir, out_name, left, right, tasks):
# NO TARGETS
trgs = []
cmd = '{0!s} quant -i {1!s} -o {2!s}/{3!s} {4!s} {5!s}'.format(
tool_path_check(TOOLS_DICT['kallisto'].full_exe[0]), index, out_dir,
out_name, left, right)
name = 'kallisto'
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def build_kallisto_task(opc, assembly_path, assembly_name, out_dir, tasks):
# NO TARGETS
trgs = []
cmd = '{0!s} index -i {1!s}/{2!s}_kallisto {3!s}'.format(
tool_path_check(TOOLS_DICT['kallisto'].full_exe[0]), out_dir,
assembly_name, assembly_path)
name = 'build_kallisto'
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def cat_task(opc, out_dir, left, right, basename, tasks):
trgs = [
'{0!s}/{1!s}_1.fastq'.format(out_dir, basename),
'{0!s}/{1!s}_2.fastq'.format(out_dir, basename)
]
cmd = 'cat {0!s} > {1!s}; cat {2!s} > {3!s}'.format(
' '.join(left), trgs[0], ' '.join(right), trgs[1])
name = 'cat_basename'
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
name=name,
stdout=out,
stderr=err,
targets=trgs)
def express_task(opc, bowtie2_index, assembly_path, out_dir, out_name,
bam_input, tasks):
trgs = ['{0!s}/{1!s}.xprs'.format(out_dir, out_name)]
cmd = ('mkdir {1!s}/{2!s}; {0!s} --output-dir {1!s}/{2!s} {3!s} {4!s}; mv '
'{1!s}/{2!s}/results.xprs {5!s}; rm -rf {1!s}/{2!s};').format(
tool_path_check(TOOLS_DICT['express'].full_exe[0]), out_dir,
out_name, assembly_path, bam_input, trgs[0])
name = 'express_' + os.path.basename(bowtie2_index) + '_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def intersect_bed_task(opc, out_dir, bam_file, bed_reference, output_name,
tasks):
trgs = ['{0!s}/{1!s}.bed'.format(out_dir, output_name)]
# cmd = '{0!s} intersect -abam {1!s} -b {2!s} -wb -bed > {3!s}'.format(
cmd = '{0!s} -abam {1!s} -b {2!s} -wb -bed > {3!s}'.format(
tool_path_check(TOOLS_DICT['bedtools'].full_exe[0]), bam_file,
bed_reference, trgs[0])
name = 'intersect_bed_' + os.path.basename(
bed_reference) + '_' + output_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def assembly_stats_task(opc, out_dir, assembly, tasks):
''' Defines assembly_stats task. Uses PATH_DIR, PATH_SCRIPTS, NAME_ASSEMBLY.
Params :
tasks - a list of tasks that this task is dependant on (trinity_task)
'''
trgs = ['{0!s}/assembly_stats.json'.format(out_dir)]
cmd = 'python {0!s}/assembly_stats.py {1!s} > {2!s}'.format(
statics.PATH_UTIL, assembly, trgs[0])
name = 'assembly_stats_' + os.path.basename(assembly)
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err)
def build_salmon_task(opc, path_assembly, assembly_name, out_dir, cpu_cap,
tasks):
trgs = ['{0!s}/{1!s}_salmon'.format(out_dir, assembly_name)]
cmd = ('{0!s} index --transcripts {1!s} --index {2!s}/{3!s}_salmon '
'--threads {4!s} --type quasi').format(
tool_path_check(TOOLS_DICT['salmon'].full_exe[0]),
path_assembly, out_dir, assembly_name, cpu_cap)
name = 'build_salmon_' + assembly_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err,
cpu=cpu_cap)
def bowtie2_unpaired_task(opc, bowtie2_index, out_dir, fastq, out_name, opt,
cpu_cap, tasks):
opts = ['-a -t --end-to-end', '-t --local', '-k 200 --end-to-end']
trgs = ['{0!s}/{1!s}.bam'.format(out_dir, out_name)]
cmd = ('{0!s} {1!s} -L {2!s} -N 1 --threads {3!s} -x {4!s} -U '
'{5!s} | samtools view -Sb - > {6!s} ').format(
tool_path_check(TOOLS_DICT['bowtie2'].full_exe[1]), opts[opt],
22, cpu_cap, bowtie2_index, fastq, trgs[0])
name = 'bowtie2_' + os.path.basename(bowtie2_index) + '_' + out_name
out, err = gen_logs(opc.path_logs, name)
return Task(command=cmd,
dependencies=tasks,
targets=trgs,
name=name,
stdout=out,
stderr=err,
cpu=cpu_cap)
