def main():
usage = "usage: %prog [Options]"
parser = OptionParser(usage=usage)
parser.add_option("-r", "--root", metavar="PATH", help="PATH to the root of the hierarchy", action="store", type="string", dest="root")
parser.add_option("-c", "--category", metavar="CATEGORY", help="name of the category from %s" % constants.CATEGORY, action="store", choices=constants.CATEGORY, dest="category")
(options, args) = parser.parse_args()
if not (options.root and options.category):
parser.print_help()
sys.exit()
# get the data from Goodgle spreadsheet
lines = gdocs.getValues(doc='%s_454_Projects' % options.category.title())
# check output path
util.checkDir(options.root)
out_metadata = "%s/.tmp/metadata/%s" % (options.root, options.category)
util.checkDir(out_metadata)
out_fastq = "%s/.tmp/fastq/%s" % (options.root, options.category)
util.checkDir(out_fastq)
# open output files
sequence_index = open('%s/sequence.index' % out_metadata, 'w')
samples_info = open('%s/samples.info' % out_metadata, 'w')
assembly_index = open('%s/assembly.index' % out_metadata, 'w')
# process input data
sample_count = 0
for line in lines:
line = line.strip()
values = line.split('||')
genus = values[1]
species = values[2]
strain = values[3]
organism_name = "%s %s %s" % (genus, species, strain)
sample_count = sample_count + 1
sample = values[4]
if sample == 'None':
sample = strain
library = values[5]
run = values[6]
if library == 'None':
library = run
if values[7] == '1':
paired = 'PAIRED'
else:
paired = 'SINGLE'
log.error("Single read set for %s. Not implemented." % run)
continue
insert_size = values[8]
sff_file = "/nfs/%s" % values[9]
trim_status = "/nfs/%s/454TrimStatus.txt" % values[10]
contigs_file = "/nfs/%s/454LargeContigs.fna" %values[10]
scaffolds_file = "/nfs/%s/454Scaffolds.fna" %values[10]
species_strain = sub('[^a-zA-Z0-9_]', '_', "%s_%s" % (species, strain)).replace('__', '_')
strain_4_hierarchy = sub('[^a-zA-Z0-9_]', '_', strain).replace('__', '_')
study = "%s_%s%s" % (values[0], genus[0], species_strain)
instrument_platform = '454'
empty = 'n/a'
fastq_1_gz = "%s/%s_1.fastq.gz" % (out_fastq, run)
fastq_2_gz = "%s/%s_2.fastq.gz" % (out_fastq, run)
fastq_0_gz = "%s/%s.fastq.gz" % (out_fastq, run)
# check that project name (study) is less than 40 char
# mysql> desc project;
# | name | varchar(40) | NO | MUL | | |
# | hierarchy_name | varchar(40) | NO | MUL | | |
if len(study) > 40:
log.warning("Project name %s has more than 40 char." % study)
# checking files
util.checkFile(sff_file)
util.checkFile(trim_status)
util.checkFile(contigs_file)
util.checkFile(scaffolds_file)
log.info("> checking fastq files: %s" % run)
# get lane hierarchy path (run)
run_path = util.runProcess("/software/bin/perl /nfs/users/nfs_a/ap12/genlibpy/genepy/pathtrack/get_lane_hierarchy_path.pl --lane=%s --db=%s" % (run, constants.DATABASE[options.category])).strip()
# check if fastq files have been loaded in db
do_generate_indexes = False
do_generate_assembly_indexes = False
if run_path != "undefined":
log.info(" loaded in db.")
fastq_path = "%s/%s/seq-pipelines/%s" % (options.root, options.category, run_path)
util.checkDir(fastq_path)
# check if fastq files have been imported into the hierarchy
if not (os.path.exists("%s/%s_1.fastq.gz" % (fastq_path, run)) and os.path.exists("%s/%s_2.fastq.gz" % (fastq_path, run)) and os.path.exists("%s/%s.fastq.gz" % (fastq_path, run))):
log.info(" not imported into hierarchy.")
# check if fastq files have been generated from sff into tmp dir
if not (os.path.exists(fastq_1_gz) and os.path.exists(fastq_2_gz) and os.path.exists(fastq_0_gz)):
log.info(" not generated from sff files.")
else:
log.info(" generate indexes.")
do_generate_indexes = True
else:
log.info(" already imported into hierarchy.")
do_generate_assembly_indexes = True
else:
log.info(" not loaded in db.")
# check if fastq files have been generated from sff into tmp dir
if not (os.path.exists(fastq_1_gz) and os.path.exists(fastq_2_gz) and os.path.exists(fastq_0_gz)):
log.info(" not generated from sff files.")
else:
log.info(" generate indexes.")
do_generate_indexes = True
# generate sequence and sample indexes
if do_generate_indexes:
# calculate md5
md5_1 = util.runProcess("md5sum %s | cut -d ' ' -f 1" % fastq_1_gz).strip()
md5_2 = util.runProcess("md5sum %s | cut -d ' ' -f 1" % fastq_2_gz).strip()
md5_0 = util.runProcess("md5sum %s | cut -d ' ' -f 1" % fastq_0_gz).strip()
# write to output files
# sequence.index: fastq_file|md5|run_id|study_id|(study_name)|center_name|(submission_id)|(submission_date)|sample_id|sample_name|
# (population)|(experiment_id)|instrument_platform|(instrument_model)|library_name|(run_name)|(run_block_name)|
# insert_size|(library_layout)|paired_fastq|withdrawn|(withdrawn_date)|(comment)|read_count|base_count
sequence_index.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\n"
% (fastq_1_gz, md5_1, run, study, study, 'SC', empty, empty, sample, sample, strain, empty, instrument_platform,
empty, library, empty, empty, insert_size, 'PAIRED', fastq_2_gz, '0', empty, empty, '0', '0'))
sequence_index.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\n"
% (fastq_2_gz, md5_2, run, study, study, 'SC', empty, empty, sample, sample, strain, empty, instrument_platform,
empty, library, empty, empty, insert_size, 'PAIRED', fastq_1_gz, '0', empty, empty, '0', '0'))
sequence_index.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\n"
% (fastq_0_gz, md5_0, run, study, study, 'SC', empty, empty, sample, sample, strain, empty, instrument_platform,
empty, library, empty, empty, insert_size, 'SINGLE', '', '0', empty, empty, '0', '0'))
# samples.info: lookup_name|acc|individual_name|alias|population_name|species_name|taxon_id|sex
samples_info.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\n" % (strain, strain, strain, strain, strain, organism_name, empty, empty))
# generate assembly indexes
if do_generate_indexes or do_generate_assembly_indexes:
# assembly.index: genus||species_strain||assembly_id||contig||scaffold||fastq
# /pyrodata01/assemblies/Ruminococcus/obeum/A2162/P_2009_07_12_22_16_23_runAssembly/454TrimStatus.txt
assembly_id = "newbler_%s" % trim_status.split('/P_')[1][:10]
assembly_index.write("%s||%s||%s||%s||%s||%s||%s\n" % (study, genus, species_strain, assembly_id, contigs_file, scaffolds_file, run))
# close files
sequence_index.close()
samples_info.close()
assembly_index.close()