def __init__(self):
super().__init__()
self.ui = ui.MainWindow()
self.ui.setupMainWindow()
self.ui.show()
self.queue = Queue()
self.frames = []
self.lines = None
self.live_thread_flag = None
self.record_thread_flag = None
self.filepath = 'D:\\Data\\'
self.filename = None
self.rescale_min = 0
self.rescale_max = 65535
self.lock = threading.Lock()
self.hcam = cam.HamamatsuCameraMR(camera_id=0)
self.ui.shutterButton.clicked.connect(lambda: self.shutterUi())
self.ui.AOTFButton.clicked.connect(lambda: self.aotfUi())
self.ui.GalvoButton.clicked.connect(lambda: self.galvoUi())
self.ui.StageButton.clicked.connect(lambda: self.stageUi())
self.ui.liveButton.clicked.connect(lambda: self.live_state_change())
self.ui.recordButton.clicked.connect(
lambda: self.record_state_change())
self.ui.IrecordButton.clicked.connect(
lambda: self.Irecord_state_change())
self.ui.autoscalebutton.clicked.connect(lambda: self.autoscale())
self.ui.slider_up.valueChanged.connect(lambda: self.upscale())
self.ui.slider_down.valueChanged.connect(lambda: self.downscale())
self.live_thread = threading.Thread(target=self.display,
name='liveThread')
self.record_thread = threading.Thread(target=self.recording,
name="recordThread")
def __init__(self, acq_duration, expos_time, focal_start, focal_interval):
threading.Thread.__init__(
self
) #initialize this class on a new thread for fastest possible capture of stacks
print " ----------- Starting Acquisition thread ------------- "
print "--> Initializing Camera Parameters"
self.hcam = hc.HamamatsuCameraMR(0)
# Set camera parameters.
cam_offset = 100
cam_x = 2048
cam_y = 2048
self.hcam.setPropertyValue("defect_correct_mode", "OFF")
self.hcam.setPropertyValue("exposure_time", expos_time)
self.hcam.setPropertyValue("subarray_hsize", cam_x)
self.hcam.setPropertyValue("subarray_vsize", cam_y)
self.hcam.setPropertyValue("binning", "1x1")
self.hcam.setPropertyValue("readout_speed", 2)
# self.cam.set(16, expos_time) # Exposure propID# is 16 Exposure as appropriate for the fluorescence intensity
# print "--> Initializing OptoTune Lens"
# self.lens = Opto(port='/dev/cu.usbmodem1411') # Initialize the optotune lens
# self.focal_interval = focal_interval
# self.lens.connect()
# self.lens.focalpower(focal_start) # Set the lens focal power to focus onto the top most z-plane
self.stop_time = time.time() + acq_duration
def __init__(self, currFoc, first_expos):
threading.Thread.__init__(self)
print " ----------- Starting Preview thread ------------- "
print "--> Initializing Camera Parameters"
self.hcam = hc.HamamatsuCameraMR(0)
# Set camera parameters.
cam_offset = 100
cam_x = 2048
cam_y = 2048
# self.expos_time = Queue(maxsize=0)
self.hcam.setPropertyValue("defect_correct_mode", "OFF")
self.hcam.setPropertyValue("exposure_time", first_expos)
self.hcam.setPropertyValue("subarray_hsize", cam_x)
self.hcam.setPropertyValue("subarray_vsize", cam_y)
self.hcam.setPropertyValue("binning", "1x1")
self.hcam.setPropertyValue("readout_speed", 2)
self.show = True
self.iv = pg.imageview.ImageView()
colors = [
(0, 0, 0),
(7, 0, 220),
(236, 0, 134),
(246, 246, 0),
(255, 255, 255),
(0, 255, 0)
]
#
cmap = pg.ColorMap(pos=np.linspace(0.0, 1.0, 6), color=colors)
self.iv.setColorMap(cmap)
self.iv.setLevels(0,65535)
self.hist = self.iv.getHistogramWidget()
self.hist.vb.enableAutoRange(self.hist.vb.YAxis, False)
self.iv.show()
def __init__(self, currFoc, first_expos):
multiprocessing.Process.__init__(self)
print " ----------- Starting Acquisition thread ------------- "
print "--> Initializing Camera Parameters"
self.hcam = hc.HamamatsuCameraMR(0)
# Set camera parameters.
cam_offset = 100
cam_x = 2048
cam_y = 2048
self.expos_time = multiprocessing.Queue(maxsize=0)
self.hcam.setPropertyValue("defect_correct_mode", "OFF")
self.hcam.setPropertyValue("exposure_time", first_expos)
self.hcam.setPropertyValue("subarray_hsize", cam_x)
self.hcam.setPropertyValue("subarray_vsize", cam_y)
self.hcam.setPropertyValue("binning", "1x1")
self.hcam.setPropertyValue("readout_speed", 2)
self.show = True
def __init__(self, **kwargs):
BaseCamera.__init__(self)
if 'exposure' not in kwargs.keys():
raise KeyError('No exposure value specified')
self.hcam = hc.HamamatsuCameraMR(0)
self.camera_open = True
cam_offset = 100
cam_x = 2048
cam_y = 2048
self.hcam.setPropertyValue("defect_correct_mode", "OFF")
self.hcam.setPropertyValue("subarray_hsize", cam_x)
self.hcam.setPropertyValue("subarray_vsize", cam_y)
self.hcam.setPropertyValue("binning", "2x2")
self.hcam.setPropertyValue("readout_speed", 2)
self.exposure = kwargs['exposure']
def __init__(self):
super().__init__()
self.ui = ui.MainWindow()
self.ui.setupMainWindow()
self.ui.show()
self.dm = None
self.queue = Queue(
) #init queue that will contain synchronous recording actions
self.frames = []
self.lines = None
self.live_thread_flag = None
self.record_thread_flag = None
self.filepath = 'D:\\Data\\'
self.filename = None
self.rescale_min = 0
self.rescale_max = 65535
self.lock = threading.Lock(
) #lock used to protect codes from multi threads
self.hcam = cam.HamamatsuCameraMR(camera_id=0) #camera handle
#connect button to functions
self.ui.shutterButton.clicked.connect(lambda: self.shutterUi())
self.ui.AOTFButton.clicked.connect(lambda: self.aotfUi())
self.ui.GalvoButton.clicked.connect(lambda: self.galvoUi())
self.ui.StageButton.clicked.connect(lambda: self.stageUi())
self.ui.DMButton.clicked.connect(lambda: self.DMUi())
self.ui.liveButton.clicked.connect(lambda: self.live_state_change())
self.ui.one_record_button.clicked.connect(
lambda: self.record_one_frame_thread())
self.ui.recordButton.clicked.connect(
lambda: self.record_state_change())
self.ui.IrecordButton.clicked.connect(
lambda: self.Irecord_state_change())
self.ui.autoscalebutton.clicked.connect(lambda: self.autoscale())
self.ui.slider_up.valueChanged.connect(lambda: self.upscale())
self.ui.slider_down.valueChanged.connect(lambda: self.downscale())
self.ui.livewindow.click_on_pixel.connect(self.getIntensityInfo)
self.ui.set_expo.clicked.connect(lambda: self.set_expo())
self.ui.set_record_expo.clicked.connect(lambda: self.set_record_expo)
self.stop_record_signal.connect(lambda: self.stop_record())
self.live_thread = threading.Thread(target=self.display,
name='liveThread')
self.record_thread = threading.Thread(target=self.recording,
name="recordThread")
def __init__(self):
super().__init__()
self.ui = ui.MainWindow()
self.ui.setupMainWindow()
self.ui.show()
self.message = message.Message()
self.frames = []
self.lines = None
self.live_thread_flag = None
self.record_thread_flag = None
self.waiting_thread_flag = None
self.filename = None
self.rescale_min = 0
self.rescale_max = 65535
self.file = None
self.lock = threading.Lock()
self.hcam = cam.HamamatsuCameraMR(camera_id=0)
'''try:
self.hcam.setPropertyValue("trigger_source",2)
except:
print("camera set external trigger mode failed")
try:
self.hcam.setPropertyValue("trigger_polarity",2)
#print(self.hcam.getPropertyText("trigger_polarity"))
except:
print("camera set positive polarity failed")'''
self.ui.shutterButton.clicked.connect(lambda: self.shutterUi())
self.ui.AOTFButton.clicked.connect(lambda: self.aotfUi())
self.ui.GalvoButton.clicked.connect(lambda: self.galvoUi())
self.ui.StageButton.clicked.connect(lambda: self.stageUi())
self.ui.set_parameter.clicked.connect(
lambda: self.set_parameter_button_pushed())
self.ui.exposurebutton.clicked.connect(lambda: self.set_exposure())
self.ui.liveButton.clicked.connect(lambda: self.live_state_change())
self.ui.recordButton.clicked.connect(
lambda: self.record_state_change())
self.ui.autoscalebutton.clicked.connect(lambda: self.autoscale())
self.live_thread = threading.Thread(target=self.living,
name='liveThread')
self.record_thread = threading.Thread(target=self.recording,
name="recordThread")
the sum of x*x for every pixel in every frame.
Hazen 10/13
"""
import numpy
import sys
import time
import hamamatsu_camera as hc
if (len(sys.argv) != 3):
print("usage: <filename> <number frames>")
exit()
hcam = hc.HamamatsuCameraMR(camera_id=0)
# Set camera parameters.
cam_offset = 0
hcam.setPropertyValue("defect_correct_mode", "OFF")
hcam.setPropertyValue("exposure_time", 0.01)
hcam.setPropertyValue("binning", "1x1")
hcam.setPropertyValue("readout_speed", 2)
if False:
cam_x = 2048
cam_y = 2048
hcam.setPropertyValue("subarray_hsize", cam_x)
hcam.setPropertyValue("subarray_vsize", cam_y)
if True:
# the sum of x*x for every pixel in every frame.
#
# Hazen 10/13
#
import numpy
import sys
import time
import hamamatsu_camera as hc
if (len(sys.argv) != 3):
print "usage: <filename> <number frames>"
exit()
hcam = hc.HamamatsuCameraMR(0)
# Set camera parameters.
cam_offset = 100
hcam.setPropertyValue("defect_correct_mode", "OFF")
hcam.setPropertyValue("exposure_time", 0.01)
hcam.setPropertyValue("binning", "1x1")
hcam.setPropertyValue("readout_speed", 2)
if 1:
cam_x = 2048
cam_y = 2048
hcam.setPropertyValue("subarray_hsize", cam_x)
hcam.setPropertyValue("subarray_vsize", cam_y)
if 0: