def piechart(self, domain_type):
pie_file = self.output[0]
pylab.figure(figsize=(5, 5))
probs, labels = ([], [])
for type in DOMAIN_TYPES:
mean_coverage = mean(
domain_type[type]) if len(domain_type[type]) > 0 else 0.0
debug(self.family_name, type, mean_coverage)
probs.append(mean_coverage)
labels.append(type) #+":%0.2f" % mean_coverage)
#ax = pylab.axes([0.6, 0.6, 0.4, 0.4])
explode = [0.05 for i in xrange(len(DOMAIN_TYPES))]
patches, texts = pylab.pie(probs,
explode=None,
labels=None,
shadow=False,
colors=DOMAIN_COLORS)
pylab.figlegend(patches,
labels,
"lower left",
fancybox=True,
markerscale=0.2)
pylab.title(self.family_name)
pylab.savefig(pie_file)
def do_alignment(cl,parse=True):
command = str(cl)
if cl.tempd == None:
cwd = tempfile.mkdtemp()
else:
cwd = cl.cwd
debug("Running mammoth",command)
p = subprocess.Popen(command, shell=True, stdout=subprocess.PIPE,stderr=open(os.devnull,"w"),cwd=cwd)
if not parse:
return p.communicate()
# Now parse output
if cl.output:
# Consume the process' pipe so we don't block
for l in p.stdout:
pass
parse_output = open(cl.output)
else:
parse_output = p.stdout
ini_psi, ini_rms, end_psi, end_rms, zscore, evalue, pred_seq, pred_ss, exp_seq, exp_ss = _parse_mammoth(parse_output)
# Wait for process to finish
rcode = p.wait()
if rcode != 0:
raise subprocess.CalledProcessError(command,rcode)
mm = MammothAlignment(ini_rms=ini_rms, ini_psi=ini_psi, end_rms=end_rms, end_psi=end_psi, zscore=zscore,
evalue=evalue, pred_seq=pred_seq, pred_ss=pred_ss, exp_seq=exp_seq, exp_ss=exp_ss)
mm._cwd = cwd
return mm
def thread_setup():
global Session
if Session == None:
clear()
e, b, s = setup()
debug("Entering subprocess setup", s, Session)
Session = s
def parse(self,output):
"""
Parse the Blast handle and append records to the output file
"""
with open(output) as handle:
runtime().debug("Parsing blast results")
blast_records = NCBIXML.parse(handle)
matches = set()
for blast_record in blast_records:
for alignment in blast_record.alignments:
for hsp in alignment.hsps:
n = float(min(hsp.align_length,blast_record.query_letters))
m = float(max(hsp.align_length,blast_record.query_letters))
if n/m < self.align_perc:
continue
if hsp.expect > self.expect:
continue
matches.add(alignment.title.split()[0][4:])
runtime().debug("Found %i hits" % len(matches))
if os.path.exists(self.output):
with open(self.output) as fasta_file:
already_found = set([r.id for r in SeqIO.parse(fasta_file, "fasta")])
debug("Found %i already" % len(matches.intersection(already_found)))
matches = matches-already_found
debug("Found %i new matches"%len(matches))
if len(matches) > 0:
with hddb.connect("ddbCommon") as cursor:
records = list(hddb.proteins(cursor, sequence_key=matches))
runtime().debug("Formatting blast results into fasta file")
with open(self.output,"a") as fasta_file:
SeqIO.write(records, fasta_file, "fasta")
def thread_setup():
global Session
if Session==None:
clear()
e,b,s = setup()
debug("Entering subprocess setup",s,Session)
Session = s
def makeDirs(path):
"Raises a PathException if the path cannot be created w/ os.error number as value"
from hpf.utilities import debug
debug("Ensuring: %s" % path)
try:
os.makedirs(path)
except OSError as e:
if e.errno != errno.EEXIST:
raise PathException("Cannot make dir", e.errno)
def __enter__(self):
from hpf.utilities.paths import ensure
ensure(self.scratch)
self.session = Session()
runtime().debug("Loading outfile")
self.filesystem_outfile = self.session.query(FilesystemOutfile).filter(FilesystemOutfile.prediction_code==self.prediction_code).first()
runtime().debug("Loading sequence")
self.sequence = self.session.query(Sequence).get(self.filesystem_outfile.sequence_key)
debug(self.prediction_code,self.filesystem_outfile,self.sequence)
return self
def _do(self):
for i,fasta in enumerate(self.records()):
try:
with NamedTemporaryFile(prefix="blast",dir=os.getcwd()) as blast_out:
self.blast(fasta,blast_out.name)
self.parse(blast_out.name)
except Exception, e:
debug("Failed to blast/parse %i record in %s" % (i,self.fasta))
debug("\t",e)
continue
def _decoy(self):
"""
Find the decoy file and copy it to the scratch directory.
"""
assert os.path.exists(self.decoy), self.decoy
debug("Found",self.decoy)
destination = self.destination
shutil.copy(self.decoy, destination)
#assert os.path.exists(destination)
debug("Copied to",destination)
return destination
def find(pattern, dir=os.getcwd()):
"""Searches a directory path and yields regular expression matches."""
import re
regex = re.compile(pattern)
for (path, dames, fnames) in os.walk(dir) :
for fn in fnames:
debug(fn)
abs = os.path.abspath(join(path, fn))
match = regex.search(abs)
if match:
yield abs
def blast(fasta, db="hpf_protein", force=False):
"""
Map families to the database.
"""
runtime().debug(fasta)
file = os.path.abspath(fasta)
base = ".".join(os.path.basename(file).split(".")[:-1])
name = base.replace("_", " ")
dir = base
subprocess.call("mkdir -p %s" % base, shell=True)
runtime().debug(dir, fasta, base)
cwd = os.getcwd()
os.chdir(dir)
try:
raise Exception(
"This has been modified like crazy, don't run as is, make sure this is correct"
)
#runtime().pushd(dir)
#formatted_fasta = FormatFastaTask(file,base+".fasta").run(force=force)
# Cluster the families representatives before blasting everything to HPF
#cdhit_fasta = CdhitTask(formatted_fasta,formatted_fasta+".cdhit",identity=0.7,length=0.7).run()
#alignment = MuscleTask(formatted_fasta, base+".aln", clwstrict=True).run(force=force)
#alignment = FormatAlignmentTask(alignment,base+".alnf").run(force=force)
#blast_xml, blast_chk = InputAlignmentBlastTask(formatted_fasta,alignment, db="hpf_protein").run(force=force)
#blast_matches = BlastParseXMLTask(blast_xml, base+".hpf.fasta", 0.8, expect=1e-6).run(force=force)
#blast_matches = BlastTask(cdhit_fasta,alignment,base+".hpf.fasta").run(force=force)
#graphics = DomainGraphicsTask(base,blast_matches,base+".svg","","").run(force=force)
blast_matches = base + ".hpf.fasta"
session = Session()
family = session.query(Family).filter(Family.name == name).one()
debug(family)
with open(blast_matches) as handle:
for record in SeqIO.parse(handle, "fasta"):
map = session.query(FamilySequence).filter(
and_(FamilySequence.family_key == family.id,
FamilySequence.sequence_key == int(
record.id))).first()
if map == None:
map = FamilySequence()
map.family_key = family.id
map.sequence_key = int(record.id)
debug(map)
session.add(map)
session.commit()
session.close()
#runtime().popd()
finally:
os.chdir(cwd)
def _do(self):
for i, fasta in enumerate(self.records()):
try:
with NamedTemporaryFile(prefix="blast",
dir=os.getcwd()) as blast_out:
self.blast(fasta, blast_out.name)
self.parse(blast_out.name)
except Exception, e:
debug("Failed to blast/parse %i record in %s" %
(i, self.fasta))
debug("\t", e)
continue
def _db(self):
"""
Get the prediction from the database and write it to a file.
"""
debug("Getting psipred from database")
from hpf.pdb.psipred import PsipredWriter
psipred = self.psipred
with open(psipred,"w") as handle:
PsipredWriter().write(handle,
self.sequence.psipred.psipred,
self.sequence.record)
#assert os.path.exists(psipred)
return psipred
def blast(fasta,db="hpf_protein",force=False):
"""
Map families to the database.
"""
runtime().debug(fasta)
file = os.path.abspath(fasta)
base = ".".join(os.path.basename(file).split(".")[:-1])
name = base.replace("_"," ")
dir = base
subprocess.call("mkdir -p %s"%base, shell=True)
runtime().debug(dir,fasta,base)
cwd = os.getcwd()
os.chdir(dir)
try:
raise Exception("This has been modified like crazy, don't run as is, make sure this is correct")
#runtime().pushd(dir)
#formatted_fasta = FormatFastaTask(file,base+".fasta").run(force=force)
# Cluster the families representatives before blasting everything to HPF
#cdhit_fasta = CdhitTask(formatted_fasta,formatted_fasta+".cdhit",identity=0.7,length=0.7).run()
#alignment = MuscleTask(formatted_fasta, base+".aln", clwstrict=True).run(force=force)
#alignment = FormatAlignmentTask(alignment,base+".alnf").run(force=force)
#blast_xml, blast_chk = InputAlignmentBlastTask(formatted_fasta,alignment, db="hpf_protein").run(force=force)
#blast_matches = BlastParseXMLTask(blast_xml, base+".hpf.fasta", 0.8, expect=1e-6).run(force=force)
#blast_matches = BlastTask(cdhit_fasta,alignment,base+".hpf.fasta").run(force=force)
#graphics = DomainGraphicsTask(base,blast_matches,base+".svg","","").run(force=force)
blast_matches = base+".hpf.fasta"
session = Session()
family = session.query(Family).filter(Family.name==name).one()
debug(family)
with open(blast_matches) as handle:
for record in SeqIO.parse(handle, "fasta"):
map = session.query(FamilySequence).filter(and_(FamilySequence.family_key==family.id, FamilySequence.sequence_key==int(record.id))).first()
if map == None:
map = FamilySequence()
map.family_key=family.id
map.sequence_key = int(record.id)
debug(map)
session.add(map)
session.commit()
session.close()
#runtime().popd()
finally:
os.chdir(cwd)
def import_family(file):
name = ".".join(os.path.basename(file).split(".")[:-1]).replace("_"," ")
debug(name, file)
session = Session()
family = session.query(Family).filter(Family.name==name).first()
if family==None:
family = Family()
family.name = name
family.experiment_key = E_ID
session.add(family)
session.flush()
with open(file) as handle:
for record in SeqIO.parse(handle,"fasta"):
seq = str(record.seq).replace("*","")
if not all([c in string.ascii_uppercase for c in seq]):
runtime().println("Malformed Sequence", Family, seq)
sha1 = hashlib.sha1(seq).hexdigest()
sequence = session.query(Sequence).filter(Sequence.sha1==sha1).first()
if sequence==None:
sequence = Sequence()
sequence.sha1 = sha1
sequence.sequence=seq
id = session.add(sequence)
session.flush()
debug("Added",sequence)
sequenceAc = session.query(SequenceAc).filter(and_(SequenceAc.sequence_key==sequence.id, SequenceAc.ac==record.id)).first()
if sequenceAc==None:
sequenceAc = SequenceAc()
sequenceAc.sequence_key=sequence.id
sequenceAc.gi = None
sequenceAc.db = "amnh"
sequenceAc.ac = record.id
sequenceAc.description = "amnh"
sequenceAc.taxonomy_id = 0
session.add(sequenceAc)
session.flush()
protein = session.query(Protein).filter(and_(Protein.sequence_key==sequence.id,Protein.experiment_key==E_ID)).first()
if protein==None:
protein=Protein()
protein.experiment_key=E_ID
protein.protein_type="phylogeny"
protein.sequence_key = sequence.id
protein.probability = 0
protein.comment = "auto added amnh families"
protein.file_key = 0
protein.parse_key = 0
protein.gene_key = 0
session.add(protein)
session.flush()
debug("Added",protein)
if not family in sequence.families:
sequence.families.append(family)
session.flush()
session.commit()
session.close()
debug("closed")
return None
def piechart(self, domain_type):
pie_file = self.output[0]
pylab.figure(figsize=(5,5))
probs,labels = ([],[])
for type in DOMAIN_TYPES:
mean_coverage = mean(domain_type[type]) if len(domain_type[type])>0 else 0.0
debug(self.family_name,type,mean_coverage)
probs.append(mean_coverage)
labels.append(type)#+":%0.2f" % mean_coverage)
#ax = pylab.axes([0.6, 0.6, 0.4, 0.4])
explode = [0.05 for i in xrange(len(DOMAIN_TYPES))]
patches, texts = pylab.pie(probs,explode=None,labels=None,shadow=False,colors=DOMAIN_COLORS)
pylab.figlegend(patches, labels, "lower left", fancybox=True, markerscale=0.2)
pylab.title(self.family_name)
pylab.savefig(pie_file)
def annotate_records(fasta):
"""Attach length of domain_type coverage over each protein in fasta file"""
with open(fasta) as handle:
records = list(SeqIO.parse(handle, "fasta"))
protein = {}
for r in records:
protein[int(r.id)] = r
r.annotations['domain_type'] = defaultdict(lambda: 0)
with hddb.connect("ddbCommon") as cursor:
query = """select d.parent_sequence_key, d.domain_type, sum(length(sequence))
from domain d join sequence s on d.domain_sequence_key=s.id
where parent_sequence_key in (%s)
group by domain_type""" % ",".join([str(id) for id in protein.keys()])
debug(query)
cursor.execute(query)
for protein_key, domain_type, dlen in cursor.fetchall():
debug(len(protein[protein_key].seq), domain_type, dlen)
protein[protein_key].annotations['domain_type'][domain_type] = dlen
return protein.values()
def annotate_records(fasta):
"""Attach length of domain_type coverage over each protein in fasta file"""
with open(fasta) as handle:
records = list(SeqIO.parse(handle, "fasta"))
protein = {}
for r in records:
protein[int(r.id)] = r
r.annotations['domain_type'] = defaultdict(lambda: 0)
with hddb.connect("ddbCommon") as cursor:
query = """select d.parent_sequence_key, d.domain_type, sum(length(sequence))
from domain d join sequence s on d.domain_sequence_key=s.id
where parent_sequence_key in (%s)
group by domain_type""" % ",".join([str(id) for id in protein.keys()])
debug(query)
cursor.execute(query)
for protein_key, domain_type, dlen in cursor.fetchall():
debug(len(protein[protein_key].seq),domain_type,dlen)
protein[protein_key].annotations['domain_type'][domain_type] = dlen
return protein.values()
def parse(self, output):
"""
Parse the Blast handle and append records to the output file
"""
with open(output) as handle:
runtime().debug("Parsing blast results")
blast_records = NCBIXML.parse(handle)
matches = set()
for blast_record in blast_records:
for alignment in blast_record.alignments:
for hsp in alignment.hsps:
n = float(
min(hsp.align_length, blast_record.query_letters))
m = float(
max(hsp.align_length, blast_record.query_letters))
if n / m < self.align_perc:
continue
if hsp.expect > self.expect:
continue
matches.add(alignment.title.split()[0][4:])
runtime().debug("Found %i hits" % len(matches))
if os.path.exists(self.output):
with open(self.output) as fasta_file:
already_found = set(
[r.id for r in SeqIO.parse(fasta_file, "fasta")])
debug("Found %i already" %
len(matches.intersection(already_found)))
matches = matches - already_found
debug("Found %i new matches" % len(matches))
if len(matches) > 0:
with hddb.connect("ddbCommon") as cursor:
records = list(hddb.proteins(cursor, sequence_key=matches))
runtime().debug("Formatting blast results into fasta file")
with open(self.output, "a") as fasta_file:
SeqIO.write(records, fasta_file, "fasta")
def plot(families, filename="ginzu.svg"):
#pylab.figure(figsize=(7,7))
families.sort(cmp=lambda x,y: cmp(x[1]['psiblast'], y[1]['psiblast']))
fp = font.FontProperties(size="x-small")
ax = pylab.axes([0.3, 0.0, .6, .7])
ind = arange(len(families))
width = .35
plots = {}
domain_dict = {}
for type in DOMAIN_TYPES:
domain_dict[type] = []
names = []
for name,domains in families:
names.append(name)
for type in DOMAIN_TYPES:
domain_dict[type].append(domains[type])
sum_bottom = [0 for i in arange(len(families))]
debug("length of sum_bottom:", len(sum_bottom))
for dkey in DOMAIN_TYPES:
#plots[dkey] = pylab.bar(ind, domain_dict[dkey], color=self.color[dkey],bottom=sum_bottom)
plots[dkey] = pylab.barh(ind, domain_dict[dkey], color=colors[dkey],left=sum_bottom)
sum_bottom = map(sum, zip(sum_bottom,domain_dict[dkey]))
#pylab.xticks(ind+width/2, organisms_dict.keys(), rotation=45)
pylab.yticks(ind+width/2, names, size='xx-small')
pylab.xticks()
pylab.title("Ginzu domain frequencies")
pylab.legend( [plots[key][0] for key in DOMAIN_TYPES], DOMAIN_TYPES, prop=fp, markerscale=.5, loc=(.85,.85))
#kdrew: a little filename manipulation
#f_parts = self.yield_plot_filename.rpartition('.')
#filename = f_parts[0] + "_" + self.experiment_key + "." + f_parts[2]
pylab.savefig(filename)
def _psipred(self):
"""
Run psipred on the sequence.
"""
debug("Running psipred prediction")
from hpf.seq import TemporaryRecordFile
from hpf.pdb.psipred import Psipred, PsipredOptions
from Bio import SeqIO
fasta = os.path.join(self.scratch,self.prediction_code,self.prediction_code+".fasta")
with open(fasta,"w") as handle:
SeqIO.write([self.sequence.record], handle, "fasta")
psipred = self.psipred
from Bio.Blast.NCBIStandalone import blastpgp
chk = fasta+".chk"
import subprocess
cmd = subprocess.Popen(["which", "blastpgp"], stdout=subprocess.PIPE).communicate()[0].strip()
debug("Using",cmd)
result,error = blastpgp(cmd,
"nr",
fasta,
npasses=3,
checkpoint_outfile=chk,
expectation=1e-4,
model_threshold=1e-4,
align_outfile="/dev/null")
debug(result.readlines())
debug(error.readlines())
options = PsipredOptions(fasta,
profile=chk,
output=psipred+".1",
output2=psipred+".2",
horiz=psipred,
cwd = os.path.join(self.scratch,self.prediction_code))
prediction = Psipred(options).run()
db_pred = PsipredFactory().create(prediction,sequence_key=self.sequence.id)
self.session.add(db_pred)
self.session.commit()
#assert os.path.exists(psipred)
return psipred
def run(self):
from hpf.utilities.paths import ensure
if self.filesystem_outfile.executable_key==179:
runtime().debug("HPF1 format",self.filesystem_outfile)
_format = self.hpf1
else:
runtime().debug("HPF2 format",self.filesystem_outfile)
_format = self.hpf2
debug("Using decoy format",_format)
self.decoy = format(_format, self.prediction_code)
if not os.path.exists(self.decoy):
debug("NO DECOY FILE",self.prediction_code,self.decoy)
#return None
else:
self.destination = os.path.join(self.scratch,os.path.basename(self.decoy))
if not os.path.exists(self.destination):
ensure(os.path.join(self.scratch,self.prediction_code))
self.decoy = self._decoy()
else:
debug("exists",self.destination)
self.decoy = self.destination
self.psipred = os.path.join(self.scratch,self.prediction_code,self.prediction_code+".psipred")
if not os.path.exists(self.psipred):
ensure(os.path.join(self.scratch,self.prediction_code))
self.psipred = self._ss()
else:
debug("exists",self.psipred)
if all(map(os.path.exists,[self.decoy,self.psipred])):
runtime().debug("Exported",(self.decoy,self.psipred))
return (self.decoy,self.psipred)
else:
runtime().debug("Failed to export")
return None
def import_family(file):
name = ".".join(os.path.basename(file).split(".")[:-1]).replace("_", " ")
debug(name, file)
session = Session()
family = session.query(Family).filter(Family.name == name).first()
if family == None:
family = Family()
family.name = name
family.experiment_key = E_ID
session.add(family)
session.flush()
with open(file) as handle:
for record in SeqIO.parse(handle, "fasta"):
seq = str(record.seq).replace("*", "")
if not all([c in string.ascii_uppercase for c in seq]):
runtime().println("Malformed Sequence", Family, seq)
sha1 = hashlib.sha1(seq).hexdigest()
sequence = session.query(Sequence).filter(
Sequence.sha1 == sha1).first()
if sequence == None:
sequence = Sequence()
sequence.sha1 = sha1
sequence.sequence = seq
id = session.add(sequence)
session.flush()
debug("Added", sequence)
sequenceAc = session.query(SequenceAc).filter(
and_(SequenceAc.sequence_key == sequence.id,
SequenceAc.ac == record.id)).first()
if sequenceAc == None:
sequenceAc = SequenceAc()
sequenceAc.sequence_key = sequence.id
sequenceAc.gi = None
sequenceAc.db = "amnh"
sequenceAc.ac = record.id
sequenceAc.description = "amnh"
sequenceAc.taxonomy_id = 0
session.add(sequenceAc)
session.flush()
protein = session.query(Protein).filter(
and_(Protein.sequence_key == sequence.id,
Protein.experiment_key == E_ID)).first()
if protein == None:
protein = Protein()
protein.experiment_key = E_ID
protein.protein_type = "phylogeny"
protein.sequence_key = sequence.id
protein.probability = 0
protein.comment = "auto added amnh families"
protein.file_key = 0
protein.parse_key = 0
protein.gene_key = 0
session.add(protein)
session.flush()
debug("Added", protein)
if not family in sequence.families:
sequence.families.append(family)
session.flush()
session.commit()
session.close()
debug("closed")
return None
def _blast(fasta):
thread_setup()
if runtime().opt(FORCE):
debug("Forcing re-run")
return blast(fasta,force=runtime().opt(FORCE))
def _blast(fasta):
thread_setup()
if runtime().opt(FORCE):
debug("Forcing re-run")
return blast(fasta, force=runtime().opt(FORCE))
