def zproj(imp): zpimp = ZP() zpimp.setImage(imp) zpimp.setMethod(zpimp.MAX_METHOD) zpimp.setStartSlice(1) zpimp.setStopSlice(imp.getNSlices()) zpimp.doHyperStackProjection(True) zpedimp = zpimp.getProjection() return zpedimp
def getMIP(stackImp):
"""
Create a maximum intensity projection (MIP) from an ImagePlus stack
@return ImagePlus maximum projection
"""
zp = ZProjector(stackImp)
zp.setMethod(ZProjector.MAX_METHOD)
zp.setStopSlice(stackImp.getNSlices())
zp.doHyperStackProjection(False)
return zp.getProjection()
def projectionImage(imp):
"""Returns the MIP of the specified ImagePlus (a composite stack)"""
from ij.plugin import ZProjector
roi_exists = imp.getRoi() is not None
imp.deleteRoi()
zp = ZProjector(imp)
zp.setMethod(ZProjector.MAX_METHOD)
zp.setStartSlice(1)
zp.setStopSlice(imp.getNSlices())
zp.doHyperStackProjection(True)
mip_imp = zp.getProjection()
mip_imp.setCalibration(imp.getCalibration())
if roi_exists:
mip_imp.restoreRoi()
return mip_imp
def projectionImage(imp):
"""Returns the MIP of the specified ImagePlus (a composite stack)"""
from ij.plugin import ZProjector
roi_exists = imp.getRoi() is not None
imp.deleteRoi()
zp = ZProjector(imp)
zp.setMethod(ZProjector.MAX_METHOD)
zp.setStartSlice(1)
zp.setStopSlice(imp.getNSlices())
zp.doHyperStackProjection(True)
mip_imp = zp.getProjection()
mip_imp.setCalibration(imp.getCalibration())
if roi_exists:
mip_imp.restoreRoi()
return mip_imp
def project_z(imp, method):
zp = ZProjector(imp)
if method=="max":
zp.setMethod(ZProjector.MAX_METHOD)
if method=="sum":
zp.setMethod(ZProjector.SUM_METHOD)
zp.setStopSlice(imp.getNSlices())
zp.doHyperStackProjection(True)
zpimp = zp.getProjection()
#IJ.run(imp, "Z Project...", "projection=[Max Intensity] all");
#impout = IJ.getImage()
return zpimp
def project_z(imp, method):
zp = ZProjector(imp)
if method=="max":
zp.setMethod(ZProjector.MAX_METHOD)
if method=="sum":
zp.setMethod(ZProjector.SUM_METHOD)
zp.setStopSlice(imp.getNSlices())
zp.doHyperStackProjection(True)
zpimp = zp.getProjection()
#IJ.run(imp, "Z Project...", "projection=[Max Intensity] all");
#impout = IJ.getImage()
return zpimp
def Zproj(stackimp, method, z_ind, z_range):
# Gets a stack and max project it.
# imp= stackimp.duplicate()
zp = ZProjector(stackimp)
if method == "MAX":
print "MAX"
zp.setMethod(ZProjector.MAX_METHOD)
elif method == "SUM":
print "SUM"
zp.setMethod(ZProjector.MAX_METHOD)
print "+/-", int(z_range / 2), "of z-index", z_ind
zp.setStartSlice(z_ind - int(z_range / 2))
zp.setStopSlice(z_ind + int(z_range / 2))
zp.doProjection()
zpimp = zp.getProjection()
return zpimp
def Zproj(stackimp,method,z_ind,z_range): """ Gets a single channel z-stack as imp and max project it using the provided method. stackimp(ImagePlus): a single channel z-stack as ImagePlus object z_index(int): The central stack number z_range(int): The total number of stacks surrounding the z_index to indclude """ from ij.plugin import ZProjector # imp= stackimp.duplicate() zp = ZProjector(stackimp) if method=="MAX": print "MAX" zp.setMethod(ZProjector.MAX_METHOD) elif method=="SUM": zp.setMethod(ZProjector.MAX_METHOD) zp.setStartSlice(z_ind-int(z_range/2)) zp.setStopSlice(z_ind+int(z_range/2)) zp.doProjection() zpimp = zp.getProjection() return zpimp
def glidingprojection(imp,
startframe=1,
stopframe=None,
glidingFlag=True,
no_frames_per_integral=3,
projectionmethod="Median"):
"""This function subtracts the gliding projection of several frames from the
input stack. Thus, everything which moves too fast is filtered away.
Args:
imp (ImagePlus): Input image as ImagePlus object.
startframe (int, optional): Choose a start frame. Defaults to 1.
stopframe (int, optional): Choose an end frame. Defaults to None.
glidingFlag (bool, optional): Should a gliding frame by frame projection be used? Defaults to True.
no_frames_per_integral (int, optional): Number of frames to project each integral. Defaults to 3.
projectionmethod (str, optional): Choose the projection method. Options are
'Average Intensity', 'Max Intensity', 'Min Intensity', 'Sum Slices', 'Standard Deviation', 'Median'. Defaults to "Median".
Raises:
RuntimeException: Start frame > stop frame.
Returns:
ImagePlus: The output stack.
"""
# Store some image properties.
cal = imp.getCalibration()
width, height, nChannels, nSlices, nFrames = imp.getDimensions()
title = imp.getTitle()
# Some simple sanity checks for input parameters.
if stopframe == None: stopframe = nFrames
if (startframe > stopframe):
IJ.showMessage("Start frame > Stop frame, can't go backwards in time!")
raise RuntimeException("Start frame > Stop frame!")
# If a subset of the image is to be projected, these lines of code handle that.
if ((startframe != 1) or (stopframe != nFrames)):
imp = Duplicator().run(imp, 1, nChannels, 1, nSlices, startframe,
stopframe)
# Define the number of frames to advance per step based on boolean input parameter glidingFlag.
if glidingFlag: frames_to_advance_per_step = 1
else: frames_to_advance_per_step = no_frames_per_integral
# Make a dict containg method_name:const_fieled_value pairs for the projection methods
methods_as_strings = [
'Average Intensity', 'Max Intensity', 'Min Intensity', 'Sum Slices',
'Standard Deviation', 'Median'
]
methods_as_const = [
ZProjector.AVG_METHOD, ZProjector.MAX_METHOD, ZProjector.MIN_METHOD,
ZProjector.SUM_METHOD, ZProjector.SD_METHOD, ZProjector.MEDIAN_METHOD
]
method_dict = dict(zip(methods_as_strings, methods_as_const))
# Initialize a ZProjector object and an empty stack to collect projections.
zp = ZProjector(imp)
zp.setMethod(method_dict[projectionmethod])
outstack = imp.createEmptyStack()
# Loop through all the frames in the image, and project that frame with the other frames in the integral.
for frame in range(1, nFrames + 1, frames_to_advance_per_step):
zp.setStartSlice(frame)
zp.setStopSlice(frame + no_frames_per_integral)
zp.doProjection()
outstack.addSlice(zp.getProjection().getProcessor())
# Create an image processor from the newly created Z-projection stack
# nFrames = outstack.getSize()/nChannels
impout = ImagePlus(
title + '_' + projectionmethod + '_' + str(no_frames_per_integral) +
'_frames', outstack)
impout = HyperStackConverter.toHyperStack(impout, nChannels, nSlices,
nFrames)
impout.setCalibration(cal)
return impout
options = getOptions()
if options is not None:
runCamR, runCamL, CamRScale, CamLScale, BackgroundWindowStart, BackgroundWindowEnd, GridSize, TranslateR, TranslateL = options # unpack each parameter
print runCamR, runCamL, CamRScale, CamLScale, BackgroundWindowStart, BackgroundWindowEnd, GridSize, TranslateR, TranslateL
if runCamR:
impR1 = FolderOpener.open(folder, "file=CamR sort")
UID = impR1.title
filepath = folder.strip(UID + '/')
projectR = ZProjector(impR1)
projectR.setMethod(ZProjector.AVG_METHOD)
projectR.setImage(impR1)
projectR.setStartSlice(int(BackgroundWindowStart))
projectR.setStopSlice(int(BackgroundWindowEnd))
projectR.doProjection()
projectionR = projectR.getProjection()
impR2 = ImageCalculator().run("Subtract create stack", impR1, projectionR)
IJ.run(impR2, "Enhance Contrast", "saturated=0.4 process_all")
IJ.run(impR2, "Set Scale...",
"distance=" + str(CamRScale) + " known=1 unit=cm")
IJ.run(impR2, "Grid...",
"grid=Lines area=" + str(GridSize) + " color=Cyan")
IJ.run(impR2, "Translate...",
"x=" + str(TranslateR) + " y=0 interpolation=None stack")
savepath = path.join(folder, UID + "_CR.tif")
print savepath
fs = FileSaver(impR2)
def ColMigBud():
def setupDialog(imp):
gd = GenericDialog("Collective migration buddy options")
gd.addMessage("Collective migration buddy 2.0, you are analyzing: " +
imp.getTitle())
calibration = imp.getCalibration()
if (calibration.frameInterval > 0):
default_interval = calibration.frameInterval
default_timeunit = calibration.getTimeUnit()
else:
default_interval = 8
default_timeunit = "min"
gd.addNumericField("Frame interval:", default_interval,
2) # show 2 decimals
gd.addCheckbox("Do you want to use a gliding window?", True)
gd.addCheckbox(
"Project hyperStack? (defaluts to projecting current channel only)",
False)
gd.addStringField("time unit", default_timeunit, 3)
gd.addSlider("Start compacting at frame:", 1, imp.getNFrames(), 1)
gd.addSlider("Stop compacting at frame:", 1, imp.getNFrames(),
imp.getNFrames())
gd.addNumericField("Number of frames to project in to one:", 3,
0) # show 0 decimals
gd.addChoice('Method to use for frame projection:', methods_as_strings,
methods_as_strings[5])
gd.showDialog()
if gd.wasCanceled():
IJ.log("User canceled dialog!")
return
return gd
#Start by getting the active image window and get the current active channel and other stats
imp = WindowManager.getCurrentImage()
cal = imp.getCalibration()
nSlices = 1 #TODO fix this in case you want to do Z-stacks
title = imp.getTitle()
current_channel = imp.getChannel()
#zp = ZProjector(imp)
#Make a dict containg method_name:const_fieled_value pairs for the projection methods
methods_as_strings = [
'Average Intensity', 'Max Intensity', 'Min Intensity', 'Sum Slices',
'Standard Deviation', 'Median'
]
methods_as_const = [
ZProjector.AVG_METHOD, ZProjector.MAX_METHOD, ZProjector.MIN_METHOD,
ZProjector.SUM_METHOD, ZProjector.SD_METHOD, ZProjector.MEDIAN_METHOD
]
medthod_dict = dict(zip(methods_as_strings, methods_as_const))
# Run the setupDialog, read out and store the options
gd = setupDialog(imp)
frame_interval = gd.getNextNumber()
time_unit = gd.getNextString()
glidingFlag = gd.getNextBoolean()
hyperstackFlag = gd.getNextBoolean()
#Set the frame interval and unit, and store it in the ImagePlus calibration
cal.frameInterval = frame_interval
cal.setTimeUnit(time_unit)
imp.setCalibration(cal)
start_frame = int(gd.getNextNumber())
stop_frame = int(gd.getNextNumber())
#If a subset of the image is to be projected, these lines of code handle that
if (start_frame > stop_frame):
IJ.showMessage("Start frame > Stop frame, can't go backwards in time!")
raise RuntimeException("Start frame > Stop frame!")
if ((start_frame != 1) or (stop_frame != imp.getNFrames())):
imp = Duplicator().run(imp, 1, nChannels, 1, nSlices, start_frame,
stop_frame)
no_frames_per_integral = int(gd.getNextNumber())
#the doHyperstackProjection method can't project past the end of the stack
if hyperstackFlag:
total_no_frames_to_project = imp.getNFrames() - no_frames_per_integral
#the doProjection method can project past the end, it just adds black frames at the end
#When not projecting hyperstacks, just copy the current active channel from the active image
else:
total_no_frames_to_project = imp.getNFrames()
imp = Duplicator().run(imp, current_channel, current_channel, 1,
nSlices, start_frame, stop_frame)
#The Z-Projection magic happens here through a ZProjector object
zp = ZProjector(imp)
projection_method = gd.getNextChoice()
chosen_method = medthod_dict[projection_method]
zp.setMethod(chosen_method)
outstack = imp.createEmptyStack()
if glidingFlag:
frames_to_advance_per_step = 1
else:
frames_to_advance_per_step = no_frames_per_integral
for frame in range(1, total_no_frames_to_project,
frames_to_advance_per_step):
zp.setStartSlice(frame)
zp.setStopSlice(frame + no_frames_per_integral)
if hyperstackFlag:
zp.doHyperStackProjection(False)
projected_stack = zp.getProjection().getStack()
for channel in range(projected_stack.getSize()):
outstack.addSlice(projected_stack.getProcessor(channel + 1))
else:
zp.doProjection()
outstack.addSlice(zp.getProjection().getProcessor())
#Create an image processor from the newly created Z-projection stack
nChannels = imp.getNChannels()
nFrames = outstack.getSize() / nChannels
imp2 = ImagePlus(
title + '_' + projection_method + '_' + str(no_frames_per_integral) +
'_frames', outstack)
imp2 = HyperStackConverter.toHyperStack(imp2, nChannels, nSlices, nFrames)
imp2.show()
Startmenu()
def CheckMoment(image):
#Perform a maximum projection on the stack
stack = image.getStack()
NSlices=image.getNSlices()
#Take a fixed number of central slices
i=float(NSlices-SimulationCharacteristics['CentralZSlices'])
i1=math.floor(i/2)
i2=math.ceil(i/2)
startSlice=int(1+i1)
stopSlice=int(NSlices-i2)
p=bool(1)
proj = ZProjector()
proj.setMethod(ZProjector.MAX_METHOD)
proj.setImage(image)
proj.setStartSlice(startSlice)
proj.setStopSlice(stopSlice)
proj.doHyperStackProjection(p)
imageMax = proj.getProjection()
imageMax.show()
stack = imageMax.getStack()
n_slices= stack.getSize()
#Get a Segmentation Mask List for every frame in the stack
RoiList=[]
RoiRatioList=[]
for index in range(1, n_slices+1):
ip = stack.getProcessor(index).convertToFloat()
boundRoi=SegmentMask(ip)
mask=boundRoi.getMask()
PixelsMask=mask.getPixels()
r=(-1)*float(sum(PixelsMask))/(ip.height*ip.width)
RoiList.append(boundRoi)
RoiRatioList.append(r)
# print(RoiRatioList)
#Optimize the Roi List. No 1.0 ratios
AllRois1=all(item == 1.0 for item in RoiRatioList)
NFrames=len(RoiRatioList)
if not AllRois1:
for i in range(NFrames):
if RoiRatioList[i]==1.0 or RoiList[i]==None:
#Find the next value that is not 1.0
NearestNot1=-1
prevIndex=i-1
nextIndex=i+1
Token=True #To run through the list alternating positions
while prevIndex>=0 or nextIndex<NFrames:
if prevIndex>0 and Token:
if RoiRatioList[prevIndex]<0.9999:
NearestNot1=prevIndex
break
else:
prevIndex-=1
if nextIndex<NFrames:Token=False
continue
if nextIndex<NFrames:
if RoiRatioList[nextIndex]<0.9999:
NearestNot1=nextIndex
break
else:
nextIndex+=1
if prevIndex>0: Token=True
continue
if NearestNot1!=-1:
#Change RoiList and RoiRationList for the NearestNot1 element
RoiList[i]=RoiList[NearestNot1]
RoiRatioList[i]=RoiRatioList[NearestNot1]
else:
AllRois1=True
print("No avaliable segmentation")
if not AllRois1:
RoiListMin=ModifyRoiList(RoiRatioList,RoiList)
else:
RoiListMin=[ None for i in range(len(RoiList))]
#Find Optimum Scale for the Wavelet
Gamma=endosomeCharacteristics['Gamma_media']
To=endosomeCharacteristics['A_media']
index=1 #Image 1
ipOrig = stack.getProcessor(index).convertToFloat()
boundRoi=RoiListMin[0]
MaxAmp,Ropt=AmplificationCurveMax(ipOrig,To,Gamma,boundRoi)
print("Choosen Scale",Ropt)
Moment3Array=[]
for index in range(1,n_slices+1):
print("time:"+str(index))
ip = stack.getProcessor(index).convertToFloat()
boundRoi=RoiListMin[index-1]
Skew=GetSigmaWavelet(ip,Ropt,boundRoi)
Moment3Array.append(Skew)
return Moment3Array
ip = origStack.getProcessor(index) #IJ.log(str(index) + " " + ip.toString()) # output info on current slice #IJ.run(imp, "Z Project...", "start=" + str(currStart) + " stop=" + str(currStop) + " projection=[Max Intensity]") #print "index=" + str(index) + "start=" + str(currStart) + " stop=" + str(currStop) if (currStart<1): currStart = 1 if (currStop>nSlices): currStop = nSlices zp = ZProjector(imp) zp.setMethod(ZProjector.MAX_METHOD) zp.setStartSlice(currStart) zp.setStopSlice(currStop) #zp.doHyperStackProjection(True) zp.doProjection() tmpProject = zp.getProjection() zImageProcessor = tmpProject.getProcessor() if (tmpProject==None): print "\tNULL ZProject" else: newStack.addSlice(origStack.getSliceLabel(index), zImageProcessor) currStart = index - userSlices currStop = index + userSlices #how the results as an image