def __init__(self):
self.seconds = SysActionParamTime()
self.kVoltsMinus = SysActionParamFloat(spellmanMinus.vars['V'],
name='V-')
self.kVoltsPlus = SysActionParamFloat(spellmanPlus.vars['V'],
name='V+')
SysAction.__init__(self, 'HV separation',
(self.seconds, self.kVoltsMinus, self.kVoltsPlus))
def __init__(self):
self.volume = SysActionParamFloat(range=[0, syringeUsableVolume_ul],
name='Volume',
units='ul',
value=syringeUsableVolume_ul)
self.repeat = SysActionParamInt('Repeat', [0, 100], value=1)
self.speed = SysActionParamFloat(pump.vars['Speed'], value=10)
SysAction.__init__(self, 'Sample->interface',
(self.volume, self.speed, self.repeat))
class SysActionSetFlows(SysAction):
def __init__(self):
self.flow1 = SysActionParamFloat(mfc1.vars['Flow'], name='Flow 1')
self.flow2 = SysActionParamFloat(mfc2.vars['Flow'], name='Flow 2')
self.flow3 = SysActionParamFloat(mfc3.vars['Flow'], name='Flow 3')
self.flow4 = SysActionParamFloat(mfc4.vars['Flow'], name='Flow 4')
SysAction.__init__(self, 'Set Flows', (self.flow1, self.flow2, self.flow3, self.flow4))
def Command(self):
mfc1.vars['Flow'].Set(self.flow1.Get())
mfc2.vars['Flow'].Set(self.flow2.Get())
mfc3.vars['Flow'].Set(self.flow3.Get())
mfc4.vars['Flow'].Set(self.flow4.Get())
def __init__(self):
self.seconds = SysActionParamTime(name='Time')
self.flow1 = SysActionParamFloat(mfc1.vars['Flow'], name='Flow 1')
self.flow2 = SysActionParamFloat(mfc2.vars['Flow'], name='Flow 2')
self.flow3 = SysActionParamFloat(mfc3.vars['Flow'], name='Flow 3')
self.flow4 = SysActionParamFloat(mfc4.vars['Flow'], name='Flow 4')
SysAction.__init__(self, 'Start', (self.seconds, self.flow1, self.flow2, self.flow3, self.flow4))
class ActionFlushCapillary(SysAction):
def __init__(self):
self.prs = SysActionParamFloat(pressureController.vars['P'])
self.seconds = SysActionParamTime()
self.trigger = SysActionParamInt('trigger?', [0, 1])
SysAction.__init__(self, 'Flush capillary',
(self.seconds, self.prs, self.trigger))
def Command(self):
valves.vars['anions liquids'].Set('closed')
valves.vars['cations liquids'].Set('closed')
valves.vars['pressure'].Set(pressureVenting)
valves.vars['pump/pressure'].Set(pressureToInterfaces)
valves.vars['pressure'].Set(pressureOn)
if self.trigger.Get(): ecorder.vars['trigger'].Set('on')
setPressure(self.prs.Get())
cfg.Sleep(self.seconds.Get())
pressureController.vars['P'].Set(0)
print 0
if self.trigger.Get(): ecorder.vars['trigger'].Set('off')
valves.vars['pressure'].Set(pressureVenting)
valves.vars['pump/pressure'].Set(pumpToInterfaces)
valves.vars['pressure'].Set('closed')
class ActionSample2Interfaces(SysAction):
def __init__(self):
self.volume = SysActionParamFloat(range=[0, syringeUsableVolume_ul],
name='Volume',
units='ul',
value=syringeUsableVolume_ul)
self.repeat = SysActionParamInt('Repeat', [0, 100], value=1)
self.speed = SysActionParamFloat(pump.vars['Speed'], value=10)
SysAction.__init__(self, 'Sample->interface',
(self.volume, self.speed, self.repeat))
def Command(self):
valves.vars['anions liquids'].Set(anionsSample)
valves.vars['cations liquids'].Set(cationsSample)
for _ in range(self.repeat.Get()):
supplyLiquids(self.volume.Get(), self.speed.Get())
class SetThermostat(SysAction):
def __init__(self):
self.temp = SysActionParamFloat(boxThermostat.vars['T'])
SysAction.__init__(self, 'Thermostat', (self.temp, ))
def Command(self):
boxThermostat.vars['T'].Set(self.temp.Get())
boxThermostat.vars['enable'].Set('on')
class SysActionSetPins(SysAction):
def __init__(self):
self.seconds = SysActionParamTime()
self.pinA = SysActionParamFloat(analPins.vars['A'])
self.pinB = SysActionParamFloat(analPins.vars['B'])
SysAction.__init__(self, 'Set pins',
(self.seconds, self.pinA, self.pinB))
def Command(self):
# Set pins
analPins.vars['A'].Set(self.pinA.Get())
analPins.vars['B'].Set(self.pinB.Get())
# Wait some time
cfg.Sleep(self.seconds.Get())
# Zero pins
analPins.vars['A'].Set(0)
analPins.vars['B'].Set(0)
class SysActionStart(SysAction):
def __init__(self):
self.seconds = SysActionParamTime(name='Time')
self.flow1 = SysActionParamFloat(mfc1.vars['Flow'], name='Flow 1')
self.flow2 = SysActionParamFloat(mfc2.vars['Flow'], name='Flow 2')
self.flow3 = SysActionParamFloat(mfc3.vars['Flow'], name='Flow 3')
self.flow4 = SysActionParamFloat(mfc4.vars['Flow'], name='Flow 4')
SysAction.__init__(self, 'Start', (self.seconds, self.flow1, self.flow2, self.flow3, self.flow4))
def Command(self):
mfc1.vars['Flow'].Set(self.flow1.Get())
mfc2.vars['Flow'].Set(self.flow2.Get())
mfc3.vars['Flow'].Set(self.flow3.Get())
mfc4.vars['Flow'].Set(self.flow4.Get())
cfg.Sleep(self.seconds.Get())
mfc1.vars['Flow'].Set(0)
mfc2.vars['Flow'].Set(0)
mfc3.vars['Flow'].Set(0)
mfc4.vars['Flow'].Set(0)
class ActionSeparation(SysAction):
def __init__(self):
self.seconds = SysActionParamTime()
self.kVoltsMinus = SysActionParamFloat(spellmanMinus.vars['V'],
name='V-')
self.kVoltsPlus = SysActionParamFloat(spellmanPlus.vars['V'],
name='V+')
SysAction.__init__(self, 'HV separation',
(self.seconds, self.kVoltsMinus, self.kVoltsPlus))
def Command(self):
valves.vars['pump/pressure'].Set('closed')
valves.vars['pressure'].Set('closed')
valves.vars['anions liquids'].Set(anionsBGE)
valves.vars['cations liquids'].Set(cationsBGE)
spellmanMinus.vars['I'].Set(spellmanMinus.vars['I'].range[1])
spellmanPlus.vars['I'].Set(spellmanPlus.vars['I'].range[1])
spellmanMinus.vars['enable'].Set('on')
spellmanPlus.vars['enable'].Set('on')
ecorder.vars['trigger'].Set('on')
spellmanMinus.vars['V'].Set(self.kVoltsMinus.Get())
spellmanPlus.vars['V'].Set(self.kVoltsPlus.Get())
cfg.Sleep(self.seconds.Get())
spellmanMinus.vars['V'].Set(0)
spellmanPlus.vars['V'].Set(0)
ecorder.vars['trigger'].Set('off')
spellmanMinus.vars['enable'].Set('off')
spellmanPlus.vars['enable'].Set('off')
spellmanMinus.vars['I'].Set(0)
spellmanPlus.vars['I'].Set(0)
class SysActionFillContainer(SysAction):
def __init__(self):
self.seconds = SysActionParamTime()
self.psi = SysActionParamFloat(pressureController.vars['P'])
SysAction.__init__(self, 'Fill Container', (self.seconds, self.psi))
def Command(self):
# Connect container to pressure controller
valves.vars['V1'].Set(valvePortPressure)
# Start pressure
pressureController.vars['P'].Set(self.psi.Get())
# Wait some time
cfg.Sleep(self.seconds.Get())
# Close container
valves.vars['V1'].Set('closed')
def __init__(self):
self.seconds = SysActionParamTime()
self.psi = SysActionParamFloat(pressureController.vars['P'])
SysAction.__init__(self, 'Fill Container', (self.seconds, self.psi))
def __init__(self):
self.prs = SysActionParamFloat(pressureController.vars['P'])
self.seconds = SysActionParamTime()
self.trigger = SysActionParamInt('trigger?', [0, 1])
SysAction.__init__(self, 'Flush capillary',
(self.seconds, self.prs, self.trigger))
def __init__(self):
self.temp = SysActionParamFloat(boxThermostat.vars['T'])
SysAction.__init__(self, 'Thermostat', (self.temp, ))
def __init__(self):
self.flow1 = SysActionParamFloat(mfc1.vars['Flow'], name='Flow 1')
self.flow2 = SysActionParamFloat(mfc2.vars['Flow'], name='Flow 2')
self.flow3 = SysActionParamFloat(mfc3.vars['Flow'], name='Flow 3')
self.flow4 = SysActionParamFloat(mfc4.vars['Flow'], name='Flow 4')
SysAction.__init__(self, 'Set Flows', (self.flow1, self.flow2, self.flow3, self.flow4))
def __init__(self):
self.seconds = SysActionParamTime()
self.pinA = SysActionParamFloat(analPins.vars['A'])
self.pinB = SysActionParamFloat(analPins.vars['B'])
SysAction.__init__(self, 'Set pins',
(self.seconds, self.pinA, self.pinB))