def draw_figures(bird, block, segment, hemi, e1, e2, stim_id, syllable_index, data_dir='/auto/tdrive/mschachter/data', exp=None):
# load up the experiment
if exp is None:
bird_dir = os.path.join(data_dir, bird)
exp_file = os.path.join(bird_dir, '%s.h5' % bird)
stim_file = os.path.join(bird_dir, 'stims.h5')
exp = Experiment.load(exp_file, stim_file)
seg = exp.get_segment(block, segment)
# get the start and end times of the stimulus
etable = exp.get_epoch_table(seg)
i = etable['id'] == stim_id
stim_times = zip(etable[i]['start_time'].values, etable[i]['end_time'].values)
stim_times.sort(key=operator.itemgetter(0))
stim_times = np.array(stim_times)
stim_durs = stim_times[:, 1] - stim_times[:, 0]
stim_dur = stim_durs.min()
# aggregate the LFPs across trials
lfps = list()
sample_rate = None
electrode_indices = None
for start_time,end_time in stim_times:
# get a slice of the LFP
lfp_data = exp.get_lfp_slice(seg, start_time, end_time)
electrode_indices,the_lfps,sample_rate = lfp_data[hemi]
stim_dur_i = int(stim_dur*sample_rate)
lfps.append(the_lfps[:, :stim_dur_i])
lfps = np.array(lfps)
# rescale the LFPs to they are in uV
lfps *= 1e6
# get the log spectrogram of the stimulus
start_time_0 = stim_times[0][0]
end_time_0 = stim_times[0][1]
stim_spec_t,stim_spec_freq,stim_spec = exp.get_spectrogram_slice(seg, start_time_0, end_time_0)
stim_spec_t = np.linspace(0, stim_dur, len(stim_spec_t))
stim_spec_dt = np.diff(stim_spec_t)[0]
nz = stim_spec > 0
stim_spec[nz] = 20*np.log10(stim_spec[nz]) + 100
stim_spec[stim_spec < 0] = 0
# get the amplitude envelope
amp_env = stim_spec.std(axis=0, ddof=1)
amp_env -= amp_env.min()
amp_env /= amp_env.max()
# segment the amplitude envelope into syllables
merge_thresh = int(0.002*sample_rate)
events = break_envelope_into_events(amp_env, threshold=0.05, merge_thresh=merge_thresh)
# translate the event indices into actual times
events *= stim_spec_dt
syllable_start,syllable_end,syllable_max_amp = events[syllable_index]
syllable_start -= 0.005
syllable_end += 0.010
amp_env_rs = amp_env*(stim_spec_freq.max() - stim_spec_freq.min()) + stim_spec_freq.min()
last_syllable_end = events[-1, 1] + 0.025
i1 = electrode_indices.index(e1)
i2 = electrode_indices.index(e2)
lfp1 = lfps[:, i1, :]
lfp2 = lfps[:, i2, :]
# zscore the LFP
lfp1 -= lfp1.mean()
lfp1 /= lfp1.std(ddof=1)
lfp2 -= lfp2.mean()
lfp2 /= lfp2.std(ddof=1)
ntrials,nelectrodes,nt = lfps.shape
ntrials_to_plot = 5
t = np.arange(nt) / sample_rate
# plot the stimulus and raw LFP for two electrodes
figsize = (24.0, 10)
fig = plt.figure(figsize=figsize)
plt.subplots_adjust(top=0.95, bottom=0.05, left=0.03, right=0.99, hspace=0.10)
gs = plt.GridSpec(3, 100)
ax = plt.subplot(gs[0, :60])
plot_spectrogram(stim_spec_t, stim_spec_freq, stim_spec, ax=ax, ticks=True, fmin=300, fmax=8000,
colormap='SpectroColorMap', colorbar=False)
# plt.plot(stim_spec_t, amp_env_rs, 'k-', linewidth=2.0, alpha=0.50)
plt.axvline(syllable_start, c='k', linestyle='dashed', linewidth=3.0)
plt.axvline(syllable_end, c='k', linestyle='dashed', linewidth=3.0)
plt.axis('tight')
plt.xlim(0, last_syllable_end)
# plot the first LFP (all trials)
ax = plt.subplot(gs[1, :60])
for k in range(ntrials_to_plot):
plt.plot(t, lfp1[k, :], '-', linewidth=2.0, alpha=0.75)
plt.plot(t, lfp1.mean(axis=0), 'k-', linewidth=3.0)
plt.axvline(syllable_start, c='k', linestyle='dashed', linewidth=3.0)
plt.axvline(syllable_end, c='k', linestyle='dashed', linewidth=3.0)
plt.xlabel('Time (ms)')
plt.ylabel('E%d (z-scored)' % e1)
plt.axis('tight')
plt.xlim(0, last_syllable_end)
ax = plt.subplot(gs[2, :60])
for k in range(ntrials_to_plot):
plt.plot(t, lfp2[k, :], '-', linewidth=2.0, alpha=0.75)
plt.plot(t, lfp2.mean(axis=0), 'k-', linewidth=3.0)
plt.axvline(syllable_start, c='k', linestyle='dashed', linewidth=3.0)
plt.axvline(syllable_end, c='k', linestyle='dashed', linewidth=3.0)
plt.xlabel('Time (ms)')
plt.ylabel('E%d (z-scored)' % e2)
plt.axis('tight')
plt.xlim(0, last_syllable_end)
# restrict the lfps to a single syllable
print 'syllable_start=%f, syllable_end=%f' % (syllable_start, syllable_end)
syllable_si = int(syllable_start*sample_rate)
syllable_ei = int(syllable_end*sample_rate)
lfp1 = lfp1[:, syllable_si:syllable_ei]
lfp2 = lfp2[:, syllable_si:syllable_ei]
# compute the trial averaged and mean subtracted lfps
lfp1_mean,lfp1_ms = compute_avg_and_ms(lfp1)
lfp2_mean,lfp2_ms = compute_avg_and_ms(lfp2)
psd_stats = get_psd_stats(bird, block, segment, hemi)
freqs, psd1, psd2, psd1_ms, psd2_ms, c12, c12_nonlocked, c12_total = compute_spectra_and_coherence_single_electrode(lfp1, lfp2, sample_rate, e1, e2, psd_stats=psd_stats)
lags_ms = get_lags_ms(sample_rate)
lfp_absmax = max(np.abs(lfp1_mean).max(), np.abs(lfp2_mean).max())
lfp_ms_absmax = max(np.abs(lfp1_ms).max(), np.abs(lfp2_ms).max())
ax = plt.subplot(gs[0, 65:80])
plt.axhline(0, c='k')
plt.plot(t[syllable_si:syllable_ei], lfp1_mean, 'k-', linewidth=3.0, alpha=1.)
plt.plot(t[syllable_si:syllable_ei], lfp2_mean, '-', c='#c0c0c0', linewidth=3.0)
# plt.xlabel('Time (s)')
plt.ylabel('Trial-avg LFP')
leg = custom_legend(['k', '#c0c0c0'], ['E%d' % e1, 'E%d' % e2])
plt.legend(handles=leg, fontsize='x-small')
plt.axis('tight')
plt.ylim(-lfp_absmax, lfp_absmax)
ax = plt.subplot(gs[0, 85:])
plt.axhline(0, c='k')
plt.plot(t[syllable_si:syllable_ei], lfp1_ms, 'k-', linewidth=3.0, alpha=1.)
plt.plot(t[syllable_si:syllable_ei], lfp2_ms, '-', c='#c0c0c0', linewidth=3.0)
# plt.xlabel('Time (s)')
plt.ylabel('Mean-sub LFP')
plt.legend(handles=leg, fontsize='x-small')
plt.axis('tight')
plt.ylim(-lfp_ms_absmax, lfp_ms_absmax)
psd_max = max(psd1.max(), psd2.max(), psd1_ms.max(), psd2_ms.max())
ax = plt.subplot(gs[1, 65:80])
plt.axhline(0, c='k')
plt.plot(freqs, psd1, 'k-', linewidth=3.0)
plt.plot(freqs, psd2, '-', c='#c0c0c0', linewidth=3.0)
plt.xlabel('Time (s)')
plt.ylabel('Trial-avg Power')
plt.legend(handles=leg, fontsize='x-small', loc=2)
plt.axis('tight')
# plt.ylim(0, psd_max)
ax = plt.subplot(gs[1, 85:])
plt.axhline(0, c='k')
plt.plot(freqs, psd1_ms, 'k-', linewidth=3.0)
plt.plot(freqs, psd2_ms, '-', c='#c0c0c0', linewidth=3.0)
plt.xlabel('Time (s)')
plt.ylabel('Mean-sub Power')
plt.legend(handles=leg, fontsize='x-small', loc=2)
plt.axis('tight')
# plt.ylim(0, psd_max)
ax = plt.subplot(gs[2, 65:80])
plt.axhline(0, c='k')
plt.axvline(0, c='k')
plt.plot(lags_ms, c12_total, 'k-', linewidth=3.0, alpha=0.75)
plt.plot(lags_ms, c12, '-', c='r', linewidth=3.0, alpha=0.75)
plt.plot(lags_ms, c12_nonlocked, '-', c='b', linewidth=3.0, alpha=0.75)
plt.xlabel('Lags (ms)')
plt.ylabel('Coherency')
leg = custom_legend(['k', 'r', 'b'], ['Raw', 'Trial-avg', 'Mean-sub'])
plt.legend(handles=leg, fontsize='x-small')
plt.axis('tight')
plt.ylim(-0.2, 0.3)
fname = os.path.join(get_this_dir(), 'raw+coherency.svg')
plt.savefig(fname, facecolor='w', edgecolor='none')
def draw_coherency_matrix(
bird,
block,
segment,
hemi,
stim_id,
trial,
syllable_index,
data_dir="/auto/tdrive/mschachter/data",
exp=None,
save=True,
):
# load up the experiment
if exp is None:
bird_dir = os.path.join(data_dir, bird)
exp_file = os.path.join(bird_dir, "%s.h5" % bird)
stim_file = os.path.join(bird_dir, "stims.h5")
exp = Experiment.load(exp_file, stim_file)
seg = exp.get_segment(block, segment)
# get the start and end times of the stimulus
etable = exp.get_epoch_table(seg)
i = etable["id"] == stim_id
stim_times = zip(etable[i]["start_time"].values, etable[i]["end_time"].values)
stim_times.sort(key=operator.itemgetter(0))
start_time, end_time = stim_times[trial]
stim_dur = float(end_time - start_time)
# get a slice of the LFP
lfp_data = exp.get_lfp_slice(seg, start_time, end_time)
electrode_indices, lfps, sample_rate = lfp_data[hemi]
# rescale the LFPs to they are in uV
lfps *= 1e6
# get the log spectrogram of the stimulus
stim_spec_t, stim_spec_freq, stim_spec = exp.get_spectrogram_slice(seg, start_time, end_time)
stim_spec_t = np.linspace(0, stim_dur, len(stim_spec_t))
stim_spec_dt = np.diff(stim_spec_t)[0]
nz = stim_spec > 0
stim_spec[nz] = 20 * np.log10(stim_spec[nz]) + 100
stim_spec[stim_spec < 0] = 0
# get the amplitude envelope
amp_env = stim_spec.std(axis=0, ddof=1)
amp_env -= amp_env.min()
amp_env /= amp_env.max()
# segment the amplitude envelope into syllables
merge_thresh = int(0.002 * sample_rate)
events = break_envelope_into_events(amp_env, threshold=0.05, merge_thresh=merge_thresh)
# translate the event indices into actual times
events *= stim_spec_dt
syllable_start, syllable_end, syllable_max_amp = events[syllable_index]
syllable_si = int(syllable_start * sample_rate)
syllable_ei = int(syllable_end * sample_rate)
# compute all cross and auto-correlations
if hemi == "L":
electrode_order = ROSTRAL_CAUDAL_ELECTRODES_LEFT
else:
electrode_order = ROSTRAL_CAUDAL_ELECTRODES_RIGHT
lags = np.arange(-20, 21)
lags_ms = (lags / sample_rate) * 1e3
window_fraction = 0.35
noise_db = 25.0
nelectrodes = len(electrode_order)
cross_mat = np.zeros([nelectrodes, nelectrodes, len(lags)])
for i in range(nelectrodes):
for j in range(nelectrodes):
lfp1 = lfps[i, syllable_si:syllable_ei]
lfp2 = lfps[j, syllable_si:syllable_ei]
if i != j:
x = coherency(lfp1, lfp2, lags, window_fraction=window_fraction, noise_floor_db=noise_db)
else:
x = correlation_function(lfp1, lfp2, lags)
_e1 = electrode_indices[i]
_e2 = electrode_indices[j]
i1 = electrode_order.index(_e1)
i2 = electrode_order.index(_e2)
# print 'i=%d, j=%d, e1=%d, e2=%d, i1=%d, i2=%d' % (i, j, _e1, _e2, i1, i2)
cross_mat[i1, i2, :] = x
# make a plot
figsize = (24.0, 13.5)
fig = plt.figure(figsize=figsize)
plt.subplots_adjust(top=0.95, bottom=0.05, left=0.03, right=0.99, hspace=0.10)
gs = plt.GridSpec(nelectrodes, nelectrodes)
for i in range(nelectrodes):
for j in range(i + 1):
ax = plt.subplot(gs[i, j])
plt.axhline(0, c="k")
plt.axvline(0, c="k")
_e1 = electrode_order[i]
_e2 = electrode_order[j]
plt.plot(lags_ms, cross_mat[i, j, :], "k-", linewidth=2.0)
plt.xticks([])
plt.yticks([])
plt.axis("tight")
plt.ylim(-0.5, 1.0)
if j == 0:
plt.ylabel("E%d" % electrode_order[i])
if i == nelectrodes - 1:
plt.xlabel("E%d" % electrode_order[j])
if save:
fname = os.path.join(get_this_dir(), "coherency_matrix.svg")
plt.savefig(fname, facecolor="w", edgecolor="none")
def draw_figures(bird, block, segment, hemi, e1, e2, stim_id, trial, syllable_index, data_dir='/auto/tdrive/mschachter/data', exp=None):
# load up the experiment
if exp is None:
bird_dir = os.path.join(data_dir, bird)
exp_file = os.path.join(bird_dir, '%s.h5' % bird)
stim_file = os.path.join(bird_dir, 'stims.h5')
exp = Experiment.load(exp_file, stim_file)
seg = exp.get_segment(block, segment)
# get the start and end times of the stimulus
etable = exp.get_epoch_table(seg)
i = etable['id'] == stim_id
stim_times = zip(etable[i]['start_time'].values, etable[i]['end_time'].values)
stim_times.sort(key=operator.itemgetter(0))
start_time,end_time = stim_times[trial]
stim_dur = float(end_time - start_time)
# get a slice of the LFP
lfp_data = exp.get_lfp_slice(seg, start_time, end_time, zscore=True)
electrode_indices,lfps,sample_rate = lfp_data[hemi]
# get the log spectrogram of the stimulus
stim_spec_t,stim_spec_freq,stim_spec = exp.get_spectrogram_slice(seg, start_time, end_time)
stim_spec_t = np.linspace(0, stim_dur, len(stim_spec_t))
stim_spec_dt = np.diff(stim_spec_t)[0]
nz = stim_spec > 0
stim_spec[nz] = 20*np.log10(stim_spec[nz]) + 100
stim_spec[stim_spec < 0] = 0
# get the amplitude envelope
amp_env = stim_spec.std(axis=0, ddof=1)
amp_env -= amp_env.min()
amp_env /= amp_env.max()
# segment the amplitude envelope into syllables
merge_thresh = int(0.002*sample_rate)
events = break_envelope_into_events(amp_env, threshold=0.05, merge_thresh=merge_thresh)
# translate the event indices into actual times
events *= stim_spec_dt
syllable_start,syllable_end,syllable_max_amp = events[syllable_index]
amp_env_rs = amp_env*(stim_spec_freq.max() - stim_spec_freq.min()) + stim_spec_freq.min()
last_syllable_end = events[-1, 1] + 0.025
i1 = electrode_indices.index(e1)
i2 = electrode_indices.index(e2)
lfp1 = lfps[i1, :]
lfp2 = lfps[i2, :]
t = np.arange(len(lfp1)) / sample_rate
legend = ['E%d' % e1, 'E%d' % e2]
if hemi == 'L':
electrode_order = ROSTRAL_CAUDAL_ELECTRODES_LEFT
else:
electrode_order = ROSTRAL_CAUDAL_ELECTRODES_RIGHT
# get the power spectrum stats for this site
psd_stats = get_psd_stats(bird, block, segment, hemi)
# compute the power spectra and cross coherence for all electrodes
lags_ms = get_lags_ms(sample_rate)
spectra,cross_mat = compute_spectra_and_coherence_multi_electrode_single_trial(lfps, sample_rate, electrode_indices, electrode_order, psd_stats=psd_stats)
# plot the stimulus and raw LFP for two electrodes
figsize = (24.0, 10)
fig = plt.figure(figsize=figsize)
plt.subplots_adjust(top=0.95, bottom=0.05, left=0.03, right=0.99, hspace=0.10)
ax = plt.subplot(2, 1, 1)
plot_spectrogram(stim_spec_t, stim_spec_freq, stim_spec, ax=ax, ticks=True, fmin=300, fmax=8000,
colormap='SpectroColorMap', colorbar=False)
# plt.plot(stim_spec_t, amp_env_rs, 'k-', linewidth=2.0, alpha=0.50)
plt.axvline(syllable_start, c='k', linestyle='dashed', linewidth=3.0)
plt.axvline(syllable_end, c='k', linestyle='dashed', linewidth=3.0)
plt.axis('tight')
plt.xlim(0, last_syllable_end)
ax = plt.subplot(2, 1, 2)
plt.plot(t, lfp1, 'b-', linewidth=3.0)
plt.plot(t, lfp2, 'r-', linewidth=3.0, alpha=0.7)
plt.axvline(syllable_start, c='k', linestyle='dashed', linewidth=3.0)
plt.axvline(syllable_end, c='k', linestyle='dashed', linewidth=3.0)
plt.xlabel('Time (ms)')
plt.ylabel('LFP (z-scored)')
plt.legend(legend)
plt.axis('tight')
plt.xlim(0, last_syllable_end)
fname = os.path.join(get_this_dir(), 'raw.svg')
plt.savefig(fname, facecolor='w', edgecolor='none')
# restrict the lfps to a single syllable
syllable_si = int(syllable_start*sample_rate)
syllable_ei = int(syllable_end*sample_rate)
lfp1 = lfp1[syllable_si:syllable_ei]
lfp2 = lfp2[syllable_si:syllable_ei]
# plot the two power spectra
psd_ub = 6
psd_lb = 0
i1 = electrode_order.index(e1)
i2 = electrode_order.index(e2)
a1 = spectra[i1, :]
a2 = spectra[i2, :]
freqs = get_freqs(sample_rate)
figsize = (10.0, 4.0)
fig = plt.figure(figsize=figsize)
plt.subplots_adjust(top=0.90, bottom=0.10, left=0.10, right=0.99, hspace=0.10)
ax = plt.subplot(1, 2, 1)
plt.plot(freqs, a1, 'b-', linewidth=3.0)
plt.plot(freqs, a2, 'r-', linewidth=3.0)
plt.xlabel('Frequency (Hz)')
plt.ylabel('Power (z-scored)')
plt.title('Power Spectrum')
handles = custom_legend(['b', 'r'], legend)
plt.legend(handles=handles, fontsize='small')
plt.axis('tight')
plt.ylim(psd_lb, psd_ub)
# plot the coherency
cf_lb = -0.1
cf_ub = 0.3
coh = cross_mat[i1, i2, :]
ax = plt.subplot(1, 2, 2)
plt.axhline(0, c='k')
plt.axvline(0, c='k')
plt.plot(lags_ms, coh, 'g-', linewidth=3.0)
plt.xlabel('Frequency (Hz)')
plt.title('Coherency')
plt.axis('tight')
plt.ylim(cf_lb, cf_ub)
fname = os.path.join(get_this_dir(), 'auto+cross.svg')
plt.savefig(fname, facecolor='w', edgecolor='none')
# compute all cross and auto-correlations
nelectrodes = len(electrode_order)
# make a plot
figsize = (24.0, 13.5)
fig = plt.figure(figsize=figsize)
plt.subplots_adjust(top=0.95, bottom=0.05, left=0.03, right=0.99, hspace=0.10)
gs = plt.GridSpec(nelectrodes, nelectrodes)
for i in range(nelectrodes):
for j in range(i+1):
ax = plt.subplot(gs[i, j])
plt.axhline(0, c='k')
plt.axvline(0, c='k')
_e1 = electrode_order[i]
_e2 = electrode_order[j]
clr = 'k'
if i == j:
if _e1 == e1:
clr = 'b'
elif _e1 == e2:
clr = 'r'
else:
if _e1 == e1 and _e2 == e2:
clr = 'g'
if i == j:
plt.plot(freqs, spectra[i, :], '-', c=clr, linewidth=2.0)
else:
plt.plot(lags_ms, cross_mat[i, j, :], '-', c=clr, linewidth=2.0)
plt.xticks([])
plt.yticks([])
plt.axis('tight')
if i != j:
plt.ylim(cf_lb, cf_ub)
else:
plt.axhline(0, c='k')
plt.ylim(psd_lb, psd_ub)
if j == 0:
plt.ylabel('E%d' % electrode_order[i])
if i == nelectrodes-1:
plt.xlabel('E%d' % electrode_order[j])
fname = os.path.join(get_this_dir(), 'cross_all.svg')
plt.savefig(fname, facecolor='w', edgecolor='none')