Esempio n. 1
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    def __init__(self, *args, **kwargs):
        super(OpCachedWsdt, self).__init__(*args, **kwargs)
        my_slot_names = set(
            map(lambda slot: slot.name, self.inputSlots + self.outputSlots))
        wsdt_slot_names = set(
            map(lambda slot: slot.name,
                OpWsdt.inputSlots + OpWsdt.outputSlots))
        assert wsdt_slot_names.issubset(my_slot_names), \
            "OpCachedWsdt should have all of the slots that OpWsdt has (and maybe more). "\
            "Did you add a slot to OpWsdt and forget to add it to OpCachedWsdt?"

        self._opWsdt = OpWsdt(parent=self)
        self._opWsdt.Input.connect(self.Input)
        self._opWsdt.ChannelSelection.connect(self.ChannelSelection)
        self._opWsdt.Pmin.connect(self.Pmin)
        self._opWsdt.MinMembraneSize.connect(self.MinMembraneSize)
        self._opWsdt.MinSegmentSize.connect(self.MinSegmentSize)
        self._opWsdt.SigmaMinima.connect(self.SigmaMinima)
        self._opWsdt.SigmaWeights.connect(self.SigmaWeights)
        self._opWsdt.GroupSeeds.connect(self.GroupSeeds)
        self._opWsdt.EnableDebugOutputs.connect(self.EnableDebugOutputs)

        self._opCache = OpBlockedArrayCache(parent=self)
        self._opCache.fixAtCurrent.connect(self.FreezeCache)
        self._opCache.Input.connect(self._opWsdt.Superpixels)
        self.Superpixels.connect(self._opCache.Output)
        self.CleanBlocks.connect(self._opCache.CleanBlocks)

        self._opSelectedInput = OpSingleChannelSelector(parent=self)
        self._opSelectedInput.Index.connect(self.ChannelSelection)
        self._opSelectedInput.Input.connect(self.Input)

        self._opThreshold = OpPixelOperator(parent=self)
        self._opThreshold.Input.connect(self._opSelectedInput.Output)
        self.ThresholdedInput.connect(self._opThreshold.Output)
Esempio n. 2
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    def __init__(self, *args, **kwargs):
        super(OpCachedWsdt, self).__init__(*args, **kwargs)
        my_slot_names = set(
            [slot.name for slot in self.inputSlots + self.outputSlots])
        wsdt_slot_names = set(
            [slot.name for slot in OpWsdt.inputSlots + OpWsdt.outputSlots])
        assert wsdt_slot_names.issubset(my_slot_names), (
            "OpCachedWsdt should have all of the slots that OpWsdt has (and maybe more)."
            "Did you add a slot to OpWsdt and forget to add it to OpCachedWsdt?"
        )

        self._opWsdt = OpWsdt(parent=self)
        self._opWsdt.Input.connect(self.Input)
        self._opWsdt.ChannelSelections.connect(self.ChannelSelections)
        self._opWsdt.Threshold.connect(self.Threshold)
        self._opWsdt.MinSize.connect(self.MinSize)
        self._opWsdt.Sigma.connect(self.Sigma)
        self._opWsdt.Alpha.connect(self.Alpha)
        self._opWsdt.PixelPitch.connect(self.PixelPitch)
        self._opWsdt.ApplyNonmaxSuppression.connect(
            self.ApplyNonmaxSuppression)
        self._opWsdt.InvertPixelProbabilities.connect(
            self.InvertPixelProbabilities)
        self._opWsdt.EnableDebugOutputs.connect(self.EnableDebugOutputs)

        self._opCache = OpBlockedArrayCache(parent=self)
        self._opCache.fixAtCurrent.connect(self.FreezeCache)
        self._opCache.Input.connect(self._opWsdt.Superpixels)
        self.Superpixels.connect(self._opCache.Output)
        self.CleanBlocks.connect(self._opCache.CleanBlocks)

        self._opSelectedInput = OpSumChannels(parent=self)
        self._opSelectedInput.ChannelSelections.connect(self.ChannelSelections)
        self._opSelectedInput.Input.connect(self.Input)
        self._opSelectedInput.InvertPixelProbabilities.connect(
            self.InvertPixelProbabilities)
        self.SelectedInput.connect(self._opSelectedInput.Output)

        self._opThreshold = OpPixelOperator(parent=self)
        self._opThreshold.Input.connect(self._opSelectedInput.Output)
        self.ThresholdedInput.connect(self._opThreshold.Output)
    def __init__(self, *args, **kwargs):
        super(OpFormattedDataExport, self).__init__(*args, **kwargs)
        self._dirty = True

        opSubRegion = OpSubRegion(parent=self)
        opSubRegion.Input.connect(self.Input)
        self._opSubRegion = opSubRegion

        # If normalization parameters are provided, we inject a 'drange'
        #  metadata item for downstream operators/gui to use.
        opDrangeInjection = OpMetadataInjector(parent=self)
        opDrangeInjection.Input.connect(opSubRegion.Output)
        self._opDrangeInjection = opDrangeInjection

        # Normalization and dtype conversion are performed in one step
        #  using an OpPixelOperator.
        opNormalizeAndConvert = OpPixelOperator(parent=self)
        opNormalizeAndConvert.Input.connect(opDrangeInjection.Output)
        self._opNormalizeAndConvert = opNormalizeAndConvert

        # ConvertedImage shows the full result but WITHOUT axis reordering.
        self.ConvertedImage.connect(self._opNormalizeAndConvert.Output)

        opReorderAxes = OpReorderAxes(parent=self)
        opReorderAxes.Input.connect(opNormalizeAndConvert.Output)
        self._opReorderAxes = opReorderAxes

        self.ImageToExport.connect(opReorderAxes.Output)

        self._opExportSlot = OpExportSlot(parent=self)
        self._opExportSlot.Input.connect(opReorderAxes.Output)
        self._opExportSlot.OutputFormat.connect(self.OutputFormat)

        self.ExportPath.connect(self._opExportSlot.ExportPath)
        self.FormatSelectionErrorMsg.connect(
            self._opExportSlot.FormatSelectionErrorMsg)
        self.progressSignal = self._opExportSlot.progressSignal
Esempio n. 4
0
    def __init__(self, *args, **kwargs):
        super(OpThresholdTwoLevels, self).__init__(*args, **kwargs)

        self.opReorderInput = OpReorderAxes(parent=self)
        self.opReorderInput.AxisOrder.setValue('tzyxc')
        self.opReorderInput.Input.connect(self.InputImage)

        # PROBABILITIES: Convert to float32
        self.opConvertProbabilities = OpConvertDtype( parent=self )
        self.opConvertProbabilities.ConversionDtype.setValue( np.float32 )
        self.opConvertProbabilities.Input.connect( self.opReorderInput.Output )

        # PROBABILITIES: Normalize drange to [0.0, 1.0]
        self.opNormalizeProbabilities = OpPixelOperator( parent=self )
        def normalize_inplace(a):
            drange = self.opNormalizeProbabilities.Input.meta.drange
            if drange is None or (drange[0] == 0.0 and drange[1] == 1.0):
                return a
            a[:] -= drange[0]
            a[:] = a[:]/float(( drange[1] - drange[0] ))
            return a
        self.opNormalizeProbabilities.Input.connect( self.opConvertProbabilities.Output )
        self.opNormalizeProbabilities.Function.setValue( normalize_inplace )
        
        self.opSmoother = OpAnisotropicGaussianSmoothing5d(parent=self)
        self.opSmoother.Sigmas.connect( self.SmootherSigma )
        self.opSmoother.Input.connect( self.opNormalizeProbabilities.Output )
        
        self.opSmootherCache = OpBlockedArrayCache(parent=self)
        self.opSmootherCache.BlockShape.setValue((1, None, None, None, 1))
        self.opSmootherCache.Input.connect( self.opSmoother.Output )
        
        self.opCoreChannelSelector = OpSingleChannelSelector(parent=self)
        self.opCoreChannelSelector.Index.connect( self.CoreChannel )
        self.opCoreChannelSelector.Input.connect( self.opSmootherCache.Output )
        
        self.opCoreThreshold = OpLabeledThreshold(parent=self)
        self.opCoreThreshold.Method.setValue( ThresholdMethod.SIMPLE )
        self.opCoreThreshold.FinalThreshold.connect( self.HighThreshold )
        self.opCoreThreshold.Input.connect( self.opCoreChannelSelector.Output )

        self.opCoreFilter = OpFilterLabels(parent=self)
        self.opCoreFilter.BinaryOut.setValue(False)
        self.opCoreFilter.MinLabelSize.connect( self.MinSize )
        self.opCoreFilter.MaxLabelSize.connect( self.MaxSize )
        self.opCoreFilter.Input.connect( self.opCoreThreshold.Output )
        
        self.opFinalChannelSelector = OpSingleChannelSelector(parent=self)
        self.opFinalChannelSelector.Index.connect( self.Channel )
        self.opFinalChannelSelector.Input.connect( self.opSmootherCache.Output )

        self.opSumInputs = OpMultiArrayMerger(parent=self) # see setupOutputs (below) for input connections
        self.opSumInputs.MergingFunction.setValue( sum )
        
        self.opFinalThreshold = OpLabeledThreshold(parent=self)
        self.opFinalThreshold.Method.connect( self.CurOperator )
        self.opFinalThreshold.FinalThreshold.connect( self.LowThreshold )
        self.opFinalThreshold.GraphcutBeta.connect( self.Beta )
        self.opFinalThreshold.CoreLabels.connect( self.opCoreFilter.Output )
        self.opFinalThreshold.Input.connect( self.opSumInputs.Output )
        
        self.opFinalFilter = OpFilterLabels(parent=self)
        self.opFinalFilter.BinaryOut.setValue(False)
        self.opFinalFilter.MinLabelSize.connect( self.MinSize )
        self.opFinalFilter.MaxLabelSize.connect( self.MaxSize )
        self.opFinalFilter.Input.connect( self.opFinalThreshold.Output )

        self.opReorderOutput = OpReorderAxes(parent=self)
        #self.opReorderOutput.AxisOrder.setValue('tzyxc') # See setupOutputs()
        self.opReorderOutput.Input.connect(self.opFinalFilter.Output)

        self.Output.connect( self.opReorderOutput.Output )

        self.opCache = OpBlockedArrayCache(parent=self)
        self.opCache.CompressionEnabled.setValue(True)
        self.opCache.Input.connect( self.opReorderOutput.Output )
        
        self.CachedOutput.connect( self.opCache.Output )
        self.CleanBlocks.connect( self.opCache.CleanBlocks )
        
        ## Debug outputs
        self.Smoothed.connect( self.opSmootherCache.Output )
        self.InputChannel.connect( self.opFinalChannelSelector.Output )
        self.SmallRegions.connect( self.opCoreThreshold.Output )
        self.FilteredSmallLabels.connect( self.opCoreFilter.Output )
        self.BeforeSizeFilter.connect( self.opFinalThreshold.Output )

        # Since hysteresis thresholding creates the big regions and immediately discards the bad ones,
        # we have to recreate it here if the user wants to view it as a debug layer 
        self.opBigRegionsThreshold = OpLabeledThreshold(parent=self)
        self.opBigRegionsThreshold.Method.setValue( ThresholdMethod.SIMPLE )
        self.opBigRegionsThreshold.FinalThreshold.connect( self.LowThreshold )
        self.opBigRegionsThreshold.Input.connect( self.opFinalChannelSelector.Output )
        self.BigRegions.connect( self.opBigRegionsThreshold.Output )
    def connectLane(self, laneIndex):
        """
        Override from base class.
        """
        opDataSelection = self.dataSelectionApplet.topLevelOperator.getLane(
            laneIndex)
        opWsdt = self.wsdtApplet.topLevelOperator.getLane(laneIndex)
        opEdgeTrainingWithMulticut = self.edgeTrainingWithMulticutApplet.topLevelOperator.getLane(
            laneIndex)
        opDataExport = self.dataExportApplet.topLevelOperator.getLane(
            laneIndex)

        # RAW DATA: Convert to float32
        opConvertRaw = OpConvertDtype(parent=self)
        opConvertRaw.ConversionDtype.setValue(np.float32)
        opConvertRaw.Input.connect(
            opDataSelection.ImageGroup[self.DATA_ROLE_RAW])

        # PROBABILITIES: Convert to float32
        opConvertProbabilities = OpConvertDtype(parent=self)
        opConvertProbabilities.ConversionDtype.setValue(np.float32)
        opConvertProbabilities.Input.connect(
            opDataSelection.ImageGroup[self.DATA_ROLE_PROBABILITIES])

        # PROBABILITIES: Normalize drange to [0.0, 1.0]
        opNormalizeProbabilities = OpPixelOperator(parent=self)

        def normalize_inplace(a):
            drange = opNormalizeProbabilities.Input.meta.drange
            if drange is None or (drange[0] == 0.0 and drange[1] == 1.0):
                return a
            a[:] -= drange[0]
            a[:] /= (drange[1] - drange[0])
            return a

        opNormalizeProbabilities.Input.connect(opConvertProbabilities.Output)
        opNormalizeProbabilities.Function.setValue(normalize_inplace)

        # GROUNDTRUTH: Convert to uint32, relabel, and cache
        opConvertGroundtruth = OpConvertDtype(parent=self)
        opConvertGroundtruth.ConversionDtype.setValue(np.uint32)
        opConvertGroundtruth.Input.connect(
            opDataSelection.ImageGroup[self.DATA_ROLE_GROUNDTRUTH])

        opRelabelGroundtruth = OpRelabelConsecutive(parent=self)
        opRelabelGroundtruth.Input.connect(opConvertGroundtruth.Output)

        opGroundtruthCache = OpBlockedArrayCache(parent=self)
        opGroundtruthCache.CompressionEnabled.setValue(True)
        opGroundtruthCache.Input.connect(opRelabelGroundtruth.Output)

        # watershed inputs
        opWsdt.RawData.connect(opDataSelection.ImageGroup[self.DATA_ROLE_RAW])
        opWsdt.Input.connect(opNormalizeProbabilities.Output)

        # Actual computation is done with both RawData and Probabilities
        opStackRawAndVoxels = OpSimpleStacker(parent=self)
        opStackRawAndVoxels.Images.resize(2)
        opStackRawAndVoxels.Images[0].connect(opConvertRaw.Output)
        opStackRawAndVoxels.Images[1].connect(opNormalizeProbabilities.Output)
        opStackRawAndVoxels.AxisFlag.setValue('c')

        # If superpixels are available from a file, use it.
        opSuperpixelsSelect = OpPrecomputedInput(ignore_dirty_input=True,
                                                 parent=self)
        opSuperpixelsSelect.PrecomputedInput.connect(
            opDataSelection.ImageGroup[self.DATA_ROLE_SUPERPIXELS])
        opSuperpixelsSelect.SlowInput.connect(opWsdt.Superpixels)

        # If the superpixel file changes, then we have to remove the training labels from the image
        opEdgeTraining = opEdgeTrainingWithMulticut.opEdgeTraining

        def handle_new_superpixels(*args):
            opEdgeTraining.handle_dirty_superpixels(opEdgeTraining.Superpixels)

        opDataSelection.ImageGroup[self.DATA_ROLE_SUPERPIXELS].notifyReady(
            handle_new_superpixels)
        opDataSelection.ImageGroup[self.DATA_ROLE_SUPERPIXELS].notifyUnready(
            handle_new_superpixels)

        # edge training inputs
        opEdgeTrainingWithMulticut.RawData.connect(opDataSelection.ImageGroup[
            self.DATA_ROLE_RAW])  # Used for visualization only
        opEdgeTrainingWithMulticut.VoxelData.connect(
            opStackRawAndVoxels.Output)
        opEdgeTrainingWithMulticut.Superpixels.connect(
            opSuperpixelsSelect.Output)
        opEdgeTrainingWithMulticut.GroundtruthSegmentation.connect(
            opGroundtruthCache.Output)

        # DataExport inputs
        opDataExport.RawData.connect(
            opDataSelection.ImageGroup[self.DATA_ROLE_RAW])
        opDataExport.RawDatasetInfo.connect(
            opDataSelection.DatasetGroup[self.DATA_ROLE_RAW])
        opDataExport.Inputs.resize(len(self.EXPORT_NAMES))
        opDataExport.Inputs[0].connect(opEdgeTrainingWithMulticut.Output)
        for slot in opDataExport.Inputs:
            assert slot.partner is not None