def fullScan(model,trait,chr):
### no magic number! here are constants
nInd = 1846
covFolder ='X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/Data_prep/'
phenoFolder = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/'
phenoFileName = 'Traits_S0S1.txt'
genoFolder='X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/'
outFolder= 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/outputs/'
VCdir = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/Data_prep/leave_oneGmatrix_all_families.giv/asremlCode_estimate_VC/code_for_asreml/'
covList = ['F.exp'] #a list of covariate names
phenoR = getPheno(phenoFolder, phenoFileName,trait, covList)
pheno = phenoR[1]
covariate = phenoR[2]
#print(type(covariate))
#print(covariate)
VarComps = extractVC(VCdir, trait, chr)#Vg and Verr for trait from asreml
print(VarComps)
########################## read in cov matrix
#read in the dense relationship matrix, it has no header or indicator columns
#by default loadtxt makes a float matrix
#current format of G matrix is square numeric with no row or column headings
#sort order is enforced outside of this program to match row order of genotype scores
K = np.loadtxt('X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/Gmatrix_all_families.txt.gz')
#type(K)
# read in corresponding parameters (from asreml output)
Vg = float(VarComps[0])
Ve = float(VarComps[1])
######################### error variance matrix
error = np.zeros((nInd,nInd),float)
np.fill_diagonal(error, Ve)
######################### Genetic covariance matrix
# g-cov matrix
ksigmaG = Vg*K
V = ksigmaG + error
######################### genotype
add = np.loadtxt(genoFolder+'ZeaSyn6_numeric_add_trans_shang'+"_chr"+str(chr)+'.txt',skiprows=1,dtype=str)
dom = np.loadtxt(genoFolder+'ZeaSyn6_numeric_dom_trans_shang'+"_chr"+str(chr)+'.txt',skiprows=1,dtype=str)
#add = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
#dom = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
totalMarker = add.shape[0] # shape[0] is marker number, shape[1] is individual number
f_pvalue = open(outFolder + model +trait+'pvalues'+"_chr"+str(chr)+'.txt','w',1)
f_beta = open(outFolder + model + trait+ 'beta'+"_chr"+str(chr)+'.txt','w',1)
f_dfMarker = open(outFolder + model + trait+'dfMarker'+"_chr"+str(chr)+'.txt','w',1)
count = 1
for index in range(add.shape[0]):
thisAdd = add[index,:]
thisDom = dom[index,:]
keepAddI = [item for item in range(len(thisAdd)) if thisAdd[item] != 'NA']
keepDomI = [item for item in range(len(thisDom)) if thisDom[item] != 'NA']
nonMissI = list(set(keepAddI).intersection(keepDomI))
# the following two lines confirmed that if add had NA then dom had NA too
#if(set(keepAddI) != set(keepDomI)):
#print("this one doesn't match",count)
addFilter = [float(thisAdd[i]) for i in nonMissI]
domFilter = [float(thisDom[i]) for i in nonMissI]
covFilter = [covariate[i] for i in nonMissI]
phenoFilter = [float(pheno[i][0]) for i in nonMissI]
V1=[ (np.squeeze(np.asarray(V[i]))) for i in nonMissI]
Vsub =np.asmatrix([V1[item][nonMissI] for item in range(len(V1))])
print("Vshape",Vsub.shape)
print("Do inverse OMG")
inverseV = inv(Vsub)
print("Inverse done")
result = markerTest(addFilter,domFilter,covFilter, phenoFilter, nInd, inverseV,nonMissI)
#if(model == "model5" ):
#ksigmaG9 =
#V = ksigmaG9 + error
#result = markerTest(addFilter,domFilter,covFilter, phenoFilter, nInd, V,nonMissI)
p = result[0]
beta= result[1]
print("p:",p)
print("beta",beta)
thisp = [str(p)]
if( type(beta) is int):
thisBeta = [str(beta)]
else:
thisBeta = beta.tolist()[0]
pString = "\t".join(str(x) for x in thisp)
betaString = "\t".join(str(x) for x in thisBeta)
f_pvalue.write(pString+"\n")
f_beta.write(betaString+"\n")
dfMarker=result[2]
f_dfMarker.write(str(dfMarker)+"\n")
count=count+1
print("count",count)
f_pvalue.close()
def fullScan(model, trait, DAratio, unitTime, rep):
### no magic number! here are constants
nInd = 1846
covFolder = '/home/sxue2/thirdProject/Data_prep/'
phenoFolder = '/home/sxue2/thirdProject/data/'
phenoFileName = 'allPhenoRep_' + trait + "_DAratio_" + DAratio + "_unit_" + unitTime + '.txt'
genoFolder = '/home/sxue2/thirdProject/data/'
outFolder = '/home/sxue2/thirdProject/outputs/'
VCdir = '/home/sxue2/thirdProject/data/'
covList = ['F.exp'] #a list of covariate names
repName = "rep" + str(rep)
phenoR = getPheno(phenoFolder, phenoFileName, repName, covList)
pheno = phenoR[1]
covariate = phenoR[2]
#print(type(covariate))
#print(covariate)
VarComps = [301.1166177, 2.706935e+01] #Vg and Verr for trait from asreml
########################## read in cov matrix
#read in the dense relationship matrix, it has no header or indicator columns
#by default loadtxt makes a float matrix
#current format of G matrix is square numeric with no row or column headings
#sort order is enforced outside of this program to match row order of genotype scores
K = np.loadtxt('/home/sxue2/thirdProject/data/Gmatrix_all_families.txt.gz')
#type(K)
# read in corresponding parameters (from asreml output)
Vg = float(VarComps[0])
Ve = float(VarComps[1])
######################### error variance matrix
error = np.zeros((nInd, nInd), float)
np.fill_diagonal(error, Ve)
######################### Genetic covariance matrix
# g-cov matrix
ksigmaG = Vg * K
V = ksigmaG + error
######################### genotype
add = np.loadtxt(genoFolder + 'add_' + trait + "_DAratio_" + DAratio +
"_unit_" + unitTime + '_rep_' + rep + '.txt',
skiprows=1,
dtype=str)
dom = np.loadtxt(genoFolder + 'dom_' + trait + "_DAratio_" + DAratio +
"_unit_" + unitTime + '_rep_' + rep + '.txt',
skiprows=1,
dtype=str)
#add = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
#dom = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
f_pvalue = open(
outFolder + model + trait + "_DAratio_" + DAratio + "_unit_" +
unitTime + '_rep_' + rep + 'pvalues.txt', 'w', 1)
f_beta = open(
outFolder + model + trait + "_DAratio_" + DAratio + "_unit_" +
unitTime + '_rep_' + rep + 'beta.txt', 'w', 1)
f_dfMarker = open(
outFolder + model + trait + "_DAratio_" + DAratio + "_unit_" +
unitTime + '_rep_' + rep + 'dfMarker.txt', 'w', 1)
count = 1
for index in range(add.shape[0]):
thisAdd = add[index, :]
thisDom = dom[index, :]
keepAddI = [
item for item in range(len(thisAdd)) if thisAdd[item] != 'NA'
]
keepDomI = [
item for item in range(len(thisDom)) if thisDom[item] != 'NA'
]
nonMissI = list(set(keepAddI).intersection(keepDomI))
# the following two lines confirmed that if add had NA then dom had NA too
#if(set(keepAddI) != set(keepDomI)):
#print("this one doesn't match",count)
addFilter = [float(thisAdd[i]) for i in nonMissI]
domFilter = [float(thisDom[i]) for i in nonMissI]
covFilter = [covariate[i] for i in nonMissI]
phenoFilter = [float(pheno[i][0]) for i in nonMissI]
V1 = [(np.squeeze(np.asarray(V[i]))) for i in nonMissI]
Vsub = np.asmatrix([V1[item][nonMissI] for item in range(len(V1))])
print("Vshape", Vsub.shape)
print("Do inverse OMG")
inverseV = inv(Vsub)
print("Inverse done")
result = markerTest(addFilter, domFilter, covFilter, phenoFilter, nInd,
inverseV, nonMissI)
#if(model == "model5" ):
#ksigmaG9 =
#V = ksigmaG9 + error
#result = markerTest(addFilter,domFilter,covFilter, phenoFilter, nInd, V,nonMissI)
p = result[0]
beta = result[1]
print("p:", p)
print("beta", beta)
thisp = [str(p)]
if (type(beta) is int):
thisBeta = [str(beta)]
else:
thisBeta = beta.tolist()[0]
pString = "\t".join(str(x) for x in thisp)
betaString = "\t".join(str(x) for x in thisBeta)
f_pvalue.write(pString + "\n")
f_beta.write(betaString + "\n")
dfMarker = result[2]
f_dfMarker.write(str(dfMarker) + "\n")
count = count + 1
print("count", count)
f_pvalue.close()
def fullScan(model, trait):
### no magic number! here are constants
nInd = 1846
covFolder = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/Data_prep/'
phenoFolder = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/'
phenoFileName = 'Traits_S0S1.txt'
genoFolder = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/'
outFolder = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/outputs/'
VCdir = 'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/'
covList = ['F.exp'] #a list of covariate names
phenoR = getPheno(phenoFolder, phenoFileName, trait, covList)
pheno = phenoR[1]
covariate = phenoR[2]
#print(type(covariate))
#print(covariate)
######################### genotype
add = np.loadtxt(genoFolder + 'ZeaSyn6_numeric_add_90trans.txt',
skiprows=1,
dtype=str)
dom = np.loadtxt(genoFolder + 'ZeaSyn6_numeric_dom_90trans.txt',
skiprows=1,
dtype=str)
#add = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
#dom = np.loadtxt(genoFolder+'tryadd.txt',skiprows=1,dtype=str)
totalMarker = add.shape[
0] # shape[0] is marker number, shape[1] is individual number
f_pvalue = open(outFolder + model + 'pvalues.txt', 'w', 1)
f_beta = open(outFolder + model + 'beta.txt', 'w', 1)
count = 1
for index in range(add.shape[0]):
thisAdd = add[index, :]
thisDom = dom[index, :]
keepAddI = [
item for item in range(len(thisAdd)) if thisAdd[item] != 'NA'
]
keepDomI = [
item for item in range(len(thisDom)) if thisDom[item] != 'NA'
]
nonMissI = list(set(keepAddI).intersection(keepDomI))
# the following two lines confirmed that if add had NA then dom had NA too
#if(set(keepAddI) != set(keepDomI)):
#print("this one doesn't match",count)
addFilter = [float(thisAdd[i]) for i in nonMissI]
domFilter = [float(thisDom[i]) for i in nonMissI]
covFilter = [covariate[i] for i in nonMissI]
phenoFilter = [float(pheno[i][0]) for i in nonMissI]
#########################
if (model == "model1"):
VeFixed = 500
######################### error variance matrix
error = np.zeros((nInd, nInd), float)
np.fill_diagonal(error, VeFixed)
V = error
inverseV = subsetV(V, nonMissI)
covFilter = []
result = markerTest(addFilter, domFilter, covFilter, phenoFilter,
nInd, V, nonMissI)
if (model == "model2"):
V = error
result = markerTest(addFilter, domFilter, covFilter, phenoFilter,
nInd, V, nonMissI)
if (model == "model3"):
VeEigen = 500
######################### error variance matrix
error = np.zeros((nInd, nInd), float)
np.fill_diagonal(error, VeEigen)
V = error
covList = [
'F.exp', 'Geigen1', 'Geigen2', 'Geigen3', 'Geigen4', 'Geigen5',
'Geigen6', 'Geigen7', 'Geigen8', 'Geigen9', 'Geigen10'
] #a list of covariate names
phenoR = getPheno(phenoFolder, phenoFileName, trait, covList)
covariate = phenoR[2]
result = markerTest(addFilter, domFilter, covFilter, phenoFilter,
nInd, V, nonMissI)
if (model == "model4"):
VarComps = [5492, 7053] #Vg and Verr for trait from asreml
########################## read in cov matrix
#read in the dense relationship matrix, it has no header or indicator columns
#by default loadtxt makes a float matrix
#current format of G matrix is square numeric with no row or column headings
#sort order is enforced outside of this program to match row order of genotype scores
K = np.loadtxt(
'X:/DATA/Public/shang/Ginnie-20170118T190718Z/Ginnie/data/Gmatrix_all_families.txt.gz'
)
#type(K)
# read in corresponding parameters (from asreml output)
Vg = float(VarComps[0])
Ve = float(VarComps[1])
######################### error variance matrix
error = np.zeros((nInd, nInd), float)
np.fill_diagonal(error, Ve)
######################### Genetic covariance matrix
# g-cov matrix
ksigmaG = Vg * K
V = ksigmaG + error
result = markerTest(addFilter, domFilter, covFilter, phenoFilter,
nInd, V, nonMissI)
#if(model == "model5" ):
#ksigmaG9 =
#V = ksigmaG9 + error
#result = markerTest(addFilter,domFilter,covFilter, phenoFilter, nInd, V,nonMissI)
p = result[0]
beta = result[1]
print("p:", p)
print("beta", beta)
thisp = [str(p)]
if (type(beta) is int):
thisBeta = [str(beta)]
else:
thisBeta = beta.tolist()[0]
pString = "\t".join(str(x) for x in thisp)
betaString = "\t".join(str(x) for x in thisBeta)
f_pvalue.write(pString + "\n")
f_beta.write(betaString + "\n")
count = count + 1
print("count", count)
f_pvalue.close()
