def test_relative_position(self):
# all 9 cases plus major and minor variations of overlapping, abutting and gapped
# different_ref_name = 'Samples come from different reference contigs.'
# forward_overlap = 'The end of s1 overlaps the start of s2.'
# reverse_overlap = 'The end of s2 overlaps the start of s1.'
# forward_abutted = 'The end of s1 abuts the start of s2.'
# reverse_abutted = 'The end of s2 abuts the start of s1.'
# forward_gapped = 's2 follows s1 with a gab inbetween.'
# reverse_gapped = 's1 follows s2 with a gab inbetween.'
# s2_within_s1 = 's2 is fully contained within s1.'
# s1_within_s2 = 's1 is fully contained within s2.'
slices = [
slice(0, 3), # (0,0) -> (1,0) in self.samples[0]
slice(3, 5), # (2,0) -> (2,1) in self.samples[0]
slice(5, 8), # (2,2) -> (4,0) in self.samples[0]
slice(8, 11) # (4.1) -> (4.3) in self.samples[0]
]
sliced = [self.samples[0].slice(sl) for sl in slices]
sample_dict = self.samples[0]._asdict()
sample_dict['ref_name'] = 'other'
sample_other = Sample(**sample_dict)
samples_expt = [
([self.samples[0], sample_other], Relationship.different_ref_name),
(self.samples[:2],
Relationship.forward_overlap), # overlap of minor positions
(self.samples[1:],
Relationship.forward_overlap), # overlap of major positions
(self.samples[:2][::-1],
Relationship.reverse_overlap), # overlap of minor positions
(self.samples[1:][::-1],
Relationship.reverse_overlap), # overlap of major positions
(sliced[:2], Relationship.forward_abutted), # (1,0) -> (2,0)
(sliced[1:3], Relationship.forward_abutted), # (2,1) -> (2,2)
(sliced[::-1][2:], Relationship.reverse_abutted), # (2,0) -> (1,0)
(sliced[::-1][1:3],
Relationship.reverse_abutted), # (2,2) -> (2,1)
([sliced[0],
sliced[2]], Relationship.forward_gapped), # (1,0) -> (2,2)
([self.samples[0].slice(slice(0, 4)),
sliced[2]], Relationship.forward_gapped), # (2,0) -> (2,2)
([sliced[0], self.samples[0].slice(slice(6, None))],
Relationship.forward_gapped), # (1,0) -> (3,0)
([sliced[2], self.samples[0].slice(slice(0, 4))],
Relationship.reverse_gapped), # (2,2) -> (2,0)
([self.samples[0].slice(slice(6, None)),
sliced[0]], Relationship.reverse_gapped), # (3,0) -> (1,0)
([self.samples[0], sliced[0]], Relationship.s2_within_s1),
([self.samples[0], sliced[1]], Relationship.s2_within_s1),
([self.samples[0], sliced[2]], Relationship.s2_within_s1),
([self.samples[0], sliced[3]], Relationship.s2_within_s1),
([sliced[0], self.samples[0]], Relationship.s1_within_s2),
([sliced[1], self.samples[0]], Relationship.s1_within_s2),
([sliced[2], self.samples[0]], Relationship.s1_within_s2),
([sliced[3], self.samples[0]], Relationship.s1_within_s2),
]
for samples, expt in samples_expt:
self.assertIs(Sample.relative_position(*samples), expt)
def test_from_samples(self):
# check we can concat a single sample
concat1 = Sample.from_samples([self.samples[0]])
self.assertEqual(concat1, self.samples[0])
# check we can concat 3 samples
slices = [slice(0, 5), slice(5, 8),
slice(8, 11)] # these should span entire samples[0]
sliced = [self.samples[0].slice(sl) for sl in slices]
concat3 = Sample.from_samples(sliced)
self.assertEqual(concat3, self.samples[0])
# also check raises an exception if not forward abutting
self.assertRaises(ValueError, Sample.from_samples, sliced[::-1])
def _get_sorted_index(self):
"""Get index of samples indexed by reference and ordered by start pos.
:returns: {ref_name: [sample dicts sorted by start]}
"""
ref_names = defaultdict(list)
for key, f in self.samples:
d = Sample.decode_sample_name(key)
if d is not None:
d['key'] = key
d['filename'] = f
ref_names[d['ref_name']].append(d)
# sort dicts so that refs are in order and within a ref, chunks are in order
ref_names_ordered = OrderedDict()
get_major_minor = lambda x: tuple((int(i) for i in x.split('.')))
# sort by start and -end so that if we have two samples with the same
# start but differrent end points, the longest sample comes first
sorter = lambda x: (get_major_minor(x['start']) + tuple((-i for i in get_major_minor(x['end']))))
for ref_name in sorted(ref_names.keys()):
ref_names[ref_name].sort(key=sorter)
ref_names_ordered[ref_name] = ref_names[ref_name]
return ref_names_ordered
def test_overlap_indices(self):
# check we get right thing for overlap and abutting major and minor
# and that we get an exception if we give it any other case.
slices = [
slice(0, 3), # (0,0) -> (1,0) in self.samples[0]
slice(3, 5), # (2,0) -> (2,1) in self.samples[0]
slice(5, 8), # (2,2) -> (4,0) in self.samples[0]
slice(8, 11) # (4.1) -> (4.3) in self.samples[0]
]
sliced = [self.samples[0].slice(sl) for sl in slices]
samples_expt = [
(
self.samples[:2], (9, 1, False)
), # overlap of minor inds with odd number of overlapping positions
(
self.samples[1:], (6, 2, False)
), # overlap of major inds with even number of overlapping positions
(sliced[:2], (None, None, False)), # abuts
(sliced[1:3], (None, None, False)), # abuts
(sliced[2:], (None, None, False)), # abuts
]
for samples, expt in samples_expt:
self.assertEqual(Sample.overlap_indices(*samples), expt)
self.assertRaises(OverlapException, Sample.overlap_indices, samples[1],
samples[0])
def test_gapped(self):
# check we get is_last_in_contig if we have a gap between samples
trimmed = list(Sample.trim_samples((s for s in self.samples[::2])))
for i, (expt, (got, is_last_in_contig,
heuristic)) in enumerate(zip(self.samples[::2],
trimmed)):
self.assertEqual(got, expt)
self.assertTrue(is_last_in_contig)
def test_single_sample(self):
# test that if we provide a single sample, we get the same sample back
results = list(Sample.trim_samples(iter([self.samples[0]])))
self.assertEqual(len(results), 1)
got, is_last_in_contig, heuristic = results[0]
self.assertEqual(got, self.samples[0])
self.assertTrue(is_last_in_contig)
def test_works(self):
# test simple case of 3 chained samples
trimmed = list(Sample.trim_samples((s for s in self.samples)))
for i, (expt, (got, is_last_in_contig,
heuristic)) in enumerate(zip(self.sliced, trimmed)):
self.assertEqual(got, expt)
if i == len(self.sliced) - 1:
self.assertTrue(is_last_in_contig)
else:
self.assertFalse(is_last_in_contig)
def run_prediction(output, bam, regions, model, model_file, rle_ref, read_fraction, chunk_len, chunk_ovlp,
batch_size=200, save_features=False, tag_name=None, tag_value=None, tag_keep_missing=False):
"""Inference worker."""
logger = get_named_logger('PWorker')
def sample_gen():
# chain all samples whilst dispensing with generators when done
# (they hold the feature vector in memory until they die)
for region in regions:
data_gen = SampleGenerator(
bam, region, model_file, rle_ref, read_fraction,
chunk_len=chunk_len, chunk_overlap=chunk_ovlp,
tag_name=tag_name, tag_value=tag_value,
tag_keep_missing=tag_keep_missing)
yield from data_gen.samples
batches = background_generator(
grouper(sample_gen(), batch_size), 10
)
total_region_mbases = sum(r.size for r in regions) / 1e6
logger.info("Running inference for {:.1f}M draft bases.".format(total_region_mbases))
with DataStore(output, 'a') as ds:
mbases_done = 0
t0 = now()
tlast = t0
for data in batches:
x_data = np.stack([x.features for x in data])
class_probs = model.predict_on_batch(x_data)
mbases_done += sum(x.span for x in data) / 1e6
mbases_done = min(mbases_done, total_region_mbases) # just to avoid funny log msg
t1 = now()
if t1 - tlast > 10:
tlast = t1
msg = '{:.1%} Done ({:.1f}/{:.1f} Mbases) in {:.1f}s'
logger.info(msg.format(mbases_done / total_region_mbases, mbases_done, total_region_mbases, t1 - t0))
best = np.argmax(class_probs, -1)
for sample, prob, pred, feat in zip(data, class_probs, best, x_data):
# write out positions and predictions for later analysis
sample_d = sample._asdict()
sample_d['label_probs'] = prob
sample_d['features'] = feat if save_features else None
ds.write_sample(Sample(**sample_d))
logger.info('All done')
return None
def setUpClass(cls):
pos = np.array([(0, 0), (0, 1), (1, 0), (2, 0), (2, 1), (2, 2), (3, 0),
(4, 0), (4, 1), (4, 2), (4, 3)],
dtype=[('major', int), ('minor', int)])
data_dim = 10
data = np.zeros(shape=(len(pos), data_dim))
cls.sample = Sample(ref_name='contig1',
features=data,
ref_seq=None,
labels=data,
positions=pos,
label_probs=data)
cls.file = tempfile.NamedTemporaryFile()
with datastore.DataStore(cls.file.name, 'w') as store:
store.write_sample(cls.sample)
def test_decode_sample_name(self):
expected = [
{
'ref_name': 'contig1',
'start': '0.0',
'end': '4.3'
},
{
'ref_name': 'contig1',
'start': '4.1',
'end': '7.0'
},
]
for expt, sample in zip(expected, self.samples):
self.assertEqual(expt, Sample.decode_sample_name(sample.name))
def load_sample(self, key):
"""Load `Sample` object from HDF5
:param key: str, sample name.
:returns: `Sample` object.
"""
s = {}
for field in Sample._fields:
pth = '{}/{}/{}'.format(self._sample_path_, key, field)
if pth in self.fh:
s[field] = self.fh[pth][()]
if isinstance(s[field], np.ndarray) and isinstance(s[field][0], type(b'')):
s[field] = np.char.decode(s[field])
else:
s[field] = None
return Sample(**s)
def test_030_bams_to_training_samples_simple(self):
reads_bam = tempfile.NamedTemporaryFile(suffix='.bam').name
truth_bam = tempfile.NamedTemporaryFile(suffix='.bam').name
# we had a bug caused by missing qualities and bad indexing...
data = copy.deepcopy(simple_data['calls'])
data[0]['quality'] = None
create_simple_bam(reads_bam, data)
create_simple_bam(truth_bam, [simple_data['truth']])
encoder = medaka.features.CountsFeatureEncoder(normalise='total')
label_scheme = medaka.labels.HaploidLabelScheme()
region = Region('ref', 0, 100)
result = encoder.bams_to_training_samples(truth_bam,
reads_bam,
region,
label_scheme,
min_length=0)[0]
expected = Sample(
ref_name='ref',
features=np.array(
[[0.5, 0., 0., 0., 0.5, 0., 0., 0., 0., 0.],
[0., 0.5, 0., 0., 0., 0.5, 0., 0., 0., 0.],
[0.5, 0., 0., 0., 0.5, 0., 0., 0., 0., 0.],
[0., 0.25, 0., 0.25, 0., 0., 0., 0.25, 0., 0.25],
[0.25, 0., 0., 0., 0., 0., 0., 0., 0., 0.],
[0., 0., 0.5, 0., 0., 0., 0.5, 0., 0., 0.],
[0.5, 0., 0., 0., 0.5, 0., 0., 0., 0., 0.],
[0., 0., 0., 0.5, 0., 0., 0., 0.5, 0., 0.],
[0., 0., 0.5, 0., 0., 0., 0.5, 0., 0., 0.]],
dtype='float32'),
# the two insertions with respect to the draft are dropped
labels=np.array([1, 2, 1, 4, 1, 3, 1, 4, 3]), # A C A T A G A T C
ref_seq=None,
positions=np.array([(0, 0), (1, 0), (2, 0), (3, 0), (3, 1), (4, 0),
(5, 0), (6, 0), (7, 0)],
dtype=[('major', '<i8'), ('minor', '<i8')]),
label_probs=None)
np.testing.assert_equal(result.labels, expected.labels)
np.testing.assert_equal(result.positions, expected.positions)
np.testing.assert_equal(result.features, expected.features)
def setUpClass(cls):
pos1 = np.array([(0, 0), (0, 1), (1, 0), (2, 0), (2, 1), (2, 2),
(3, 0), (4, 0), (4, 1), (4, 2), (4, 3)],
dtype=[('major', int), ('minor', int)])
pos2 = np.array([(4, 1), (4, 2), (4, 3), (4, 5), (5, 0), (6, 0),
(6, 1), (7, 0)],
dtype=[('major', int), ('minor', int)])
pos3 = np.array([(5, 0), (6, 0), (6, 1), (7, 0), (7, 1)],
dtype=[('major', int), ('minor', int)])
cls.samples = []
data_dim = 10
for pos in pos1, pos2, pos3:
data = np.random.random_sample(size=data_dim * len(pos)).reshape(
(len(pos), data_dim))
cls.samples.append(
Sample(ref_name='contig1',
features=data,
ref_seq=None,
labels=data,
positions=pos,
label_probs=data))
def _get_sorted_index(self):
"""Get index of samples indexed by reference and ordered by start pos.
:returns: {ref_name: [sample dicts sorted by start]}
"""
ref_names = defaultdict(list)
for key, f in self.samples:
d = Sample.decode_sample_name(key)
if d is not None:
d['key'] = key
d['filename'] = f
ref_names[d['ref_name']].append(d)
# sort dicts so that refs are in order and within a ref, chunks are in order
ref_names_ordered = OrderedDict()
for ref_name in sorted(ref_names.keys()):
sorter = lambda x: float(x['start'])
ref_names[ref_name].sort(key=sorter)
ref_names_ordered[ref_name] = ref_names[ref_name]
return ref_names_ordered
def test_is_empty(self):
self.assertFalse(self.samples[0].is_empty)
empty_sample = Sample('contig', None, None, None, self.samples[0][0:0],
None)
self.assertTrue(empty_sample.is_empty)
def test_raises(self):
# test we get an exception if we e.g. provide samples from different
# refs or samples out of order
self.assertRaises(OverlapException,
lambda x: list(Sample.trim_samples(x)),
iter(self.samples[::-1]))
def bam_to_sample_c(self, reads_bam, region):
"""Converts a section of an alignment pileup (as shown
by e.g. samtools tview) to a base frequency feature array
:param reads_bam: (sorted indexed) bam with read alignment to reference
:param region: `Region` object with ref_name, start and end attributes.
:param start: starting position within reference
:param end: ending position within reference
:returns: `Sample` object
"""
assert self.ref_mode is None
assert not self.consensus_as_ref
assert self.max_hp_len == 1
assert self.log_min is None
assert self.normalise == 'total' or self.normalise == 'fwd_rev' or self.normalise is None
assert not self.with_depth
assert not self.is_compressed
pileup = pileup_counts(region,
reads_bam,
dtype_prefixes=self.dtypes,
tag_name=self.tag_name,
tag_value=self.tag_value,
keep_missing=self.tag_keep_missing)
samples = list()
for counts, positions in pileup:
if len(counts) == 0:
msg = 'Pileup-feature is zero-length for {} indicating no reads in this region.'.format(
region)
self.logger.warning(msg)
samples.append(
Sample(ref_name=region.ref_name,
features=None,
labels=None,
ref_seq=None,
postions=positions,
label_probs=None))
continue
start, end = positions['major'][0], positions['major'][-1]
if start != region.start or end + 1 != region.end: # TODO investigate off-by-one
self.logger.warning(
'Pileup counts do not span requested region, requested {}, '
'received {}-{}.'.format(region, start, end))
# find the position index for parent major position of all minor positions
minor_inds = np.where(positions['minor'] > 0)
major_pos_at_minor_inds = positions['major'][minor_inds]
major_ind_at_minor_inds = np.searchsorted(positions['major'],
major_pos_at_minor_inds,
side='left')
depth = np.sum(counts, axis=1)
depth[minor_inds] = depth[major_ind_at_minor_inds]
if self.sym_indels:
# make indels at ref and non-ref positions symmetric.
# major columns otherwise have counts of reads with and without a
# deletion, whilst minor (inserted) columns only have counts of
# the reads with an isertion.
# To make ref and non-ref positions symmetric, fill in counts of reads which don't have insertions
# i.e. depth_del = depth_major - depth_ins
for (dt, is_rev), inds in self.feature_indices.items():
dt_depth = np.sum(counts[:, inds], axis=1)
del_ind = self.encoding[(dt, is_rev, None, 1)]
counts[minor_inds, del_ind] = dt_depth[
major_ind_at_minor_inds] - dt_depth[minor_inds]
if self.normalise == 'total':
# normalize counts by total depth at major position, since the
# counts include deletions this is a count of spanning reads
feature_array = counts / np.maximum(1, depth).reshape(
(-1, 1)) # max just to avoid div error
elif self.normalise == 'fwd_rev':
# normalize forward and reverse and by dtype
feature_array = np.empty_like(counts, dtype=float)
for (dt, is_rev), inds in self.feature_indices.items():
dt_depth = np.sum(counts[:, inds], axis=1)
dt_depth[minor_inds] = dt_depth[major_ind_at_minor_inds]
feature_array[:, inds] = counts[:, inds] / np.maximum(
1, dt_depth).reshape(
(-1, 1)) # max just to avoid div error
else:
feature_array = counts
feature_array = feature_array.astype(self.feature_dtype)
sample = Sample(ref_name=region.ref_name,
features=feature_array,
labels=None,
ref_seq=None,
positions=positions,
label_probs=None)
samples.append(sample)
self.logger.info('Processed {} (median depth {})'.format(
sample.name, np.median(depth)))
return samples
def bam_to_sample(self,
reads_bam,
region,
reference=None,
read_fraction=None,
force_py=False):
"""Converts a section of an alignment pileup (as shown
by e.g. samtools tview) to a base frequency feature array
:param reads_bam: (sorted indexed) bam with read alignment to reference
:param region: `Region` object with ref_name, start and end attributes.
:param reference: reference `.fasta`, should correspond to `bam`.
Required only for run length encoded references and reads.
:param read_fraction: fraction of reads to use, if `None` use all.
:param force_py: bool, if True, force use of python code (rather than c library).
:returns: iterable of `Sample` objects
"""
ref_rle = self.process_ref_seq(region.ref_name, reference)
# Try to use fast c function if we can, else fall back on this function
if not force_py and (ref_rle is None and read_fraction is None):
try:
return self.bam_to_sample_c(reads_bam, region)
except Exception as e:
self.logger.info(
'Could not process sample with bam_to_sample_c, using python code instead.\n({}).'
.format(e))
pass
if self.tag_name is not None:
raise NotImplementedError(
"Filtering alignments by tag is not supported in python code.")
#TODO: The code below will abut discontiguous regions in a pileup i.e.
# where no reads span a reference position the major position
# is dropped from the pileup. The correct behaviour would be to
# split apart the sub-regions and return them separately.
# The C implementation does this splitting.
if self.is_compressed:
aln_to_pairs = partial(yield_compressed_pairs, ref_rle=ref_rle)
elif self.max_hp_len == 1:
aln_to_pairs = get_pairs
else:
aln_to_pairs = partial(get_pairs_with_hp_len, ref_seq=ref_rle)
# accumulate data in dicts
aln_counters = defaultdict(Counter)
ref_bases = dict()
with pysam.AlignmentFile(reads_bam, 'rb') as bamfile:
aln_reads = bamfile.fetch(region.ref_name, region.start,
region.end)
if read_fraction is not None:
low, high = read_fraction
np.random.seed((int(now()) * region.start) % 2**32)
fraction = ((high - low) * np.random.random_sample(1) + low)[0]
aln_reads = [a for a in aln_reads]
n_reads = len(aln_reads)
n_reads_to_keep = max(int(fraction * n_reads), 1)
replace = n_reads_to_keep > n_reads
msg = "Resampling (replace {}) from {} to {} ({:.3f}) for {}"
self.logger.debug(
msg.format(replace, n_reads, n_reads_to_keep, fraction,
region))
aln_reads = np.random.choice(aln_reads,
n_reads_to_keep,
replace=replace)
start = region.start
end = region.end
if start is None:
start = 0
if end is None:
end = float('Inf')
for aln in aln_reads:
# get the dtype from the prefix of the query name
try:
dtype = self.dtypes[np.where([
aln.query_name.startswith(dt) for dt in self.dtypes
])[0][0]]
except:
msg = "Skipping read {} as dtype not in {}"
self.logger.info(msg.format(aln.query_name, self.dtypes))
continue
reverse = aln.is_reverse
pairs = aln_to_pairs(aln)
ins_count = 0
for pair in itertools.dropwhile(
lambda x: (x.rpos is None) or (x.rpos < start), pairs):
if ((pair.rpos == aln.reference_end - 1)
or (pair.rpos is not None and pair.rpos >= end)):
break
if pair.rpos is None:
ins_count += 1
else:
ins_count = 0
current_pos = pair.rpos
(aln_counters[(current_pos, ins_count)][self.encoding[
dtype, reverse, pair.qbase,
min(pair.qlen, self.max_hp_len)]]) += 1
ref_base = pair.rbase.upper(
) if pair.rbase is not None else '*'
ref_bases[(current_pos, ins_count)] = (ref_base, pair.rlen)
# create feature array
aln_cols = len(aln_counters)
feature_len = len(self.encoding)
feature_array = np.zeros(shape=(aln_cols, feature_len),
dtype=self.feature_dtype)
if self.log_min is not None:
feature_array.fill(np.nan)
ref_array = np.empty(shape=(aln_cols),
dtype=[('base', int), ('run_length', int)])
positions = np.empty(aln_cols,
dtype=[('major', int), ('minor', int)])
if aln_cols == 0:
msg = 'Pileup-feature is zero-length for {} indicating no reads in this region.'.format(
region)
self.logger.warning(msg)
return [
Sample(ref_name=region.ref_name,
features=None,
labels=None,
ref_seq=None,
positions=positions,
label_probs=None)
]
depth_array = np.empty(shape=(aln_cols), dtype=int)
# keep track of which features are for fwd/rev reads of each dtype
inds_by_type = self.feature_indices
#TODO: refactor so common combinations of options can be handled as in C-function
for i, ((pos, counts), (_, (ref_base, ref_len))) in \
enumerate(zip(sorted(aln_counters.items()),
sorted(ref_bases.items()))):
positions[i] = pos
ref_array[i] = (encoding[ref_base], ref_len)
for j in counts.keys():
feature_array[i, j] = counts[j]
if self.consensus_as_ref:
cons_i = np.argmax(feature_array[i])
cons_is_reverse, cons_base, cons_length = self.decoding[
cons_i]
ref_base = cons_base if cons_base is not None else _gap_
ref_len = cons_length
if positions[i]['minor'] == 0:
major_depth = sum(counts.values())
# get the depth of each fwd and rev dtype
major_depths_by_type = {
t: sum((counts[i] for i in inds_by_type[t]))
for t in inds_by_type
}
assert sum(major_depths_by_type.values()) == major_depth
if self.sym_indels and positions[i]['minor'] > 0:
# make indels at ref and non-ref positions symmetric (see comment in bam_to_sample_c).
for (dtype, is_rev), inds in inds_by_type.items():
del_ind = self.encoding[(dtype, is_rev, None, 1)]
assert feature_array[i, del_ind] == 0
feature_array[i, del_ind] = major_depths_by_type[
(dtype, is_rev)] - feature_array[i, inds].sum()
if self.normalise is not None:
if self.normalise == 'total':
feature_array[i, :] /= max(major_depth, 1)
elif self.normalise == 'fwd_rev':
# normalize fwd and reverse seperately for each dtype
for dt, inds in inds_by_type.items():
feature_array[i, inds] /= max(
major_depths_by_type[dt], 1)
depth_array[i] = major_depth
if self.with_depth:
feature_array[i, self.encoding['depth']] = depth_array[i]
if self.log_min is not None: # counts/proportions and depth will be normalised
# when we take log of probs, make it easier for network by keeping all log of
# probs positive. add self.log_min to any log probs so they are positive,
# if self.log_min is 10, we can cope with depth up to 10**9
feature_array[i, :] = np.log10(feature_array[i, :],
out=feature_array[i, :])
feature_array[i, :] += self.log_min
feature_array[i, :] = np.nan_to_num(feature_array[i, :],
copy=False)
if self.ref_mode == 'onehot':
feature_array[i, self.encoding[('ref', str(ref_base),
int(ref_len))]] = 1
elif self.ref_mode == 'base_length':
feature_array[
i, self.encoding['ref_base']] = self.ref_base_encoding[
ref_base]
feature_array[i, self.encoding['ref_length']] = ref_len
elif self.ref_mode == 'index':
# index of count which ref would contribute to were it a read
feature_array[i,
self.encoding['ref_index']] = self.encoding[(
False, min(ref_len,
self.max_hp_len), ref_base)]
sample = Sample(ref_name=region.ref_name,
features=feature_array,
labels=None,
ref_seq=ref_array,
positions=positions,
label_probs=None)
self.logger.info('Processed {} (median depth {})'.format(
sample.name, np.median(depth_array)))
return [sample]
def test_messy_overlap(self):
dtype = [('major', int), ('minor', int)]
pos = [
np.array([
(0, 0),
(1, 0),
(2, 0),
(2, 1),
(2, 2),
(3, 0),
(4, 0),
(4, 1),
(4, 2),
(4, 3),
(5, 0),
(6, 0),
(7, 0),
(8, 0),
(8, 1),
(9, 0),
],
dtype=dtype),
np.array(
[
(3, 0),
(4, 0),
(4, 1),
(4, 2), # (4,3) missing
(5, 0),
(6, 0),
(7, 0),
(8, 0),
(8, 1),
(9, 0),
(10, 0),
(10, 1),
(10, 2),
],
dtype=dtype),
np.array([
(3, 0),
(4, 0),
(4, 1),
(4, 2),
(5, 0),
(6, 0),
(6, 1),
(7, 0),
(7, 1),
(8, 0),
(8, 1),
(9, 0),
(10, 0),
(10, 1),
(10, 2),
],
dtype=dtype),
np.array(
[
(3, 0),
(4, 0),
(4, 1),
(4, 2), # (4,3) missing
(5, 0),
(5, 1),
(6, 0),
(6, 1),
(7, 0),
(7, 1),
(8, 0),
(8, 1),
(9, 0),
(10, 0),
(10, 1),
(10, 2),
],
dtype=dtype),
np.array(
[
(3, 0),
(4, 0),
(4, 1),
(4, 2), # (4,3) missing
(5, 0),
(5, 1),
(6, 0),
(6, 1),
(7, 0),
(7, 1),
(8, 0),
(9, 0),
(10, 0),
(10, 1),
(10, 2),
],
dtype=dtype),
]
sample = [
Sample(ref_name='contig1',
features=None,
ref_seq=None,
labels=None,
positions=p,
label_probs=None) for p in pos
]
expected = [
(12, 6), # (7, 0) is junction
(10, 4), # (5, 0) is junction
(13, 10), # (8, 0) is junction
(15, 11), # (9, 0) is junction
]
for other, exp in enumerate(expected, 1):
end, start, heuristic = Sample.overlap_indices(
sample[0], sample[other])
self.assertTrue(heuristic)
self.assertEqual((end, start), exp)
self.assertEqual(pos[0][exp[0]], pos[other][exp[1]])
def bams_to_training_samples(self,
truth_bam,
bam,
region,
reference=None,
read_fraction=None):
"""Prepare training data chunks.
:param truth_bam: .bam file of truth aligned to ref to generate labels.
:param bam: input .bam file.
:param region: `Region` obj.
the reference will be parsed.
:param reference: reference `.fasta`, should correspond to `bam`.
:returns: tuple of `Sample` objects.
.. note:: Chunks might be missing if `truth_bam` is provided and
regions with multiple mappings were encountered.
"""
ref_rle = self.process_ref_seq(region.ref_name, reference)
# filter truth alignments to restrict ourselves to regions of the ref where the truth
# in unambiguous
alignments = TruthAlignment.bam_to_alignments(truth_bam,
region.ref_name,
start=region.start,
end=region.end)
filtered_alignments = TruthAlignment.filter_alignments(
alignments, start=region.start, end=region.end)
if len(filtered_alignments) == 0:
self.logger.info(
"Filtering removed all alignments of truth to ref from {}.".
format(region))
samples = []
for aln in filtered_alignments:
mock_compr = self.max_hp_len > 1 and not self.is_compressed
truth_pos, truth_labels = aln.get_positions_and_labels(
ref_compr_rle=ref_rle,
mock_compr=mock_compr,
is_compressed=self.is_compressed,
rle_dtype=True)
aln_samples = self.bam_to_sample(bam,
Region(region.ref_name, aln.start,
aln.end),
ref_rle,
read_fraction=read_fraction)
for sample in aln_samples:
# Create labels according to positions in pileup
pad = (encoding[_gap_],
1) if len(truth_labels.dtype) > 0 else encoding[_gap_]
padder = itertools.repeat(pad)
position_to_label = defaultdict(
padder.__next__,
zip([tuple(p) for p in truth_pos],
[a for a in truth_labels]))
padded_labels = np.fromiter(
(position_to_label[tuple(p)] for p in sample.positions),
dtype=truth_labels.dtype,
count=len(sample.positions))
sample = sample._asdict()
sample['labels'] = padded_labels
samples.append(Sample(**sample))
return tuple(samples)