def insert_branch_mutations(ts, mutations_per_branch=1):
"""
Returns a copy of the specified tree sequence with a mutation on every branch
in every tree.
"""
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
for tree in ts.trees():
site = len(sites)
sites.add_row(position=tree.interval[0], ancestral_state='0')
for root in tree.roots:
state = {root: 0}
mutation = {root: -1}
stack = [root]
while len(stack) > 0:
u = stack.pop()
stack.extend(tree.children(u))
v = tree.parent(u)
if v != msprime.NULL_NODE:
state[u] = state[v]
parent = mutation[v]
for j in range(mutations_per_branch):
state[u] = (state[u] + 1) % 2
mutation[u] = len(mutations)
mutations.add_row(
site=site, node=u, derived_state=str(state[u]),
parent=parent)
parent = mutation[u]
tables = ts.tables
add_provenance(tables.provenances, "insert_branch_mutations")
return msprime.load_tables(
nodes=tables.nodes, edges=tables.edges, sites=sites, mutations=mutations,
provenances=tables.provenances)
def jukes_cantor(ts, num_sites, mu, multiple_per_node=True, seed=None):
"""
Returns a copy of the specified tree sequence with Jukes-Cantor mutations
applied at the specfied rate at the specifed number of sites. Site positions
are chosen uniformly.
"""
random.seed(seed)
positions = [
ts.sequence_length * random.random() for _ in range(num_sites)
]
positions.sort()
sites = msprime.SiteTable(num_sites)
mutations = msprime.MutationTable(num_sites)
trees = ts.trees()
t = next(trees)
for position in positions:
while position >= t.interval[1]:
t = next(trees)
generate_site_mutations(t,
position,
mu,
sites,
mutations,
multiple_per_node=multiple_per_node)
tables = ts.dump_tables()
add_provenance(tables.provenances, "jukes_cantor")
new_ts = msprime.load_tables(nodes=tables.nodes,
edges=tables.edges,
sites=sites,
mutations=mutations,
provenances=tables.provenances)
return new_ts
def insert_multichar_mutations(ts, seed=1, max_len=10):
"""
Returns a copy of the specified tree sequence with multiple chararacter
mutations on a randomly chosen branch in every tree.
"""
rng = random.Random(seed)
letters = ["A", "C", "T", "G"]
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
for tree in ts.trees():
site = len(sites)
ancestral_state = rng.choice(letters) * rng.randint(0, max_len)
sites.add_row(position=tree.interval[0], ancestral_state=ancestral_state)
nodes = list(tree.nodes())
nodes.remove(tree.root)
u = rng.choice(nodes)
derived_state = ancestral_state
while ancestral_state == derived_state:
derived_state = rng.choice(letters) * rng.randint(0, max_len)
mutations.add_row(site=site, node=u, derived_state=derived_state)
tables = ts.tables
add_provenance(tables.provenances, "insert_multichar_mutations")
return msprime.load_tables(
nodes=tables.nodes, edges=tables.edges, sites=sites, mutations=mutations,
provenances=tables.provenances)
def _load_legacy_hdf5_v3(root, remove_duplicate_positions):
# get the trees group for the records and samples
trees_group = root["trees"]
nodes_group = trees_group["nodes"]
time = np.array(nodes_group["time"])
breakpoints = np.array(trees_group["breakpoints"])
records_group = trees_group["records"]
left_indexes = np.array(records_group["left"])
right_indexes = np.array(records_group["right"])
record_node = np.array(records_group["node"], dtype=np.int32)
num_nodes = time.shape[0]
sample_size = np.min(record_node)
flags = np.zeros(num_nodes, dtype=np.uint32)
flags[:sample_size] = msprime.NODE_IS_SAMPLE
children_length = np.array(records_group["num_children"], dtype=np.uint32)
total_rows = np.sum(children_length)
left = np.zeros(total_rows, dtype=np.float64)
right = np.zeros(total_rows, dtype=np.float64)
parent = np.zeros(total_rows, dtype=np.int32)
record_left = breakpoints[left_indexes]
record_right = breakpoints[right_indexes]
k = 0
for j in range(left_indexes.shape[0]):
for _ in range(children_length[j]):
left[k] = record_left[j]
right[k] = record_right[j]
parent[k] = record_node[j]
k += 1
nodes = msprime.NodeTable()
nodes.set_columns(flags=flags,
time=nodes_group["time"],
population=nodes_group["population"])
edges = msprime.EdgeTable()
edges.set_columns(left=left,
right=right,
parent=parent,
child=records_group["children"])
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
if "mutations" in root:
_convert_hdf5_mutations(root["mutations"], sites, mutations,
remove_duplicate_positions)
old_timestamp = datetime.datetime.min.isoformat()
provenances = msprime.ProvenanceTable()
if "provenance" in root:
for record in root["provenance"]:
provenances.add_row(timestamp=old_timestamp, record=record)
provenances.add_row(_get_upgrade_provenance(root))
msprime.sort_tables(nodes=nodes,
edges=edges,
sites=sites,
mutations=mutations)
return msprime.load_tables(nodes=nodes,
edges=edges,
sites=sites,
mutations=mutations,
provenances=provenances)
def make_tree_add_mutations(nodes, edges, mutrate):
rng = msprime.RandomGenerator(42)
m = msprime.MutationTable()
s = msprime.SiteTable()
mg = msprime.MutationGenerator(rng, mutrate)
mg.generate(nodes, edges, s, m)
rv = msprime.load_tables(nodes=nodes, edgesets=edges, sites=s, mutations=m)
return (rv, s)
def general_mutation_example():
ts = msprime.simulate(10, recombination_rate=1, length=10, random_seed=2)
nodes = msprime.NodeTable()
edges = msprime.EdgeTable()
ts.dump_tables(nodes=nodes, edges=edges)
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
sites.add_row(position=0, ancestral_state="A", metadata=b"{}")
sites.add_row(position=1, ancestral_state="C", metadata=b"{'id':1}")
mutations.add_row(site=0, node=0, derived_state="T")
mutations.add_row(site=1, node=0, derived_state="G")
return msprime.load_tables(
nodes=nodes, edges=edges, sites=sites, mutations=mutations)
def test_sites(self):
nodes = msprime.NodeTable()
edges = msprime.EdgeTable()
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
metadata = ExampleMetadata(one="node1", two="node2")
pickled = pickle.dumps(metadata)
sites.add_row(position=0.1, ancestral_state="A", metadata=pickled)
ts = msprime.load_tables(
nodes=nodes, edges=edges, sites=sites, mutations=mutations,
sequence_length=1)
site = ts.site(0)
self.assertEqual(site.position, 0.1)
self.assertEqual(site.ancestral_state, "A")
self.assertEqual(site.metadata, pickled)
unpickled = pickle.loads(site.metadata)
self.assertEqual(unpickled.one, metadata.one)
self.assertEqual(unpickled.two, metadata.two)
def permute_nodes(ts, node_map):
"""
Returns a copy of the specified tree sequence such that the nodes are
permuted according to the specified map.
"""
# Mapping from nodes in the new tree sequence back to nodes in the original
reverse_map = [0 for _ in node_map]
for j in range(ts.num_nodes):
reverse_map[node_map[j]] = j
old_nodes = list(ts.nodes())
new_nodes = msprime.NodeTable()
for j in range(ts.num_nodes):
old_node = old_nodes[reverse_map[j]]
new_nodes.add_row(flags=old_node.flags,
metadata=old_node.metadata,
population=old_node.population,
time=old_node.time)
new_edges = msprime.EdgeTable()
for edge in ts.edges():
new_edges.add_row(left=edge.left,
right=edge.right,
parent=node_map[edge.parent],
child=node_map[edge.child])
new_sites = msprime.SiteTable()
new_mutations = msprime.MutationTable()
for site in ts.sites():
new_sites.add_row(position=site.position,
ancestral_state=site.ancestral_state)
for mutation in site.mutations:
new_mutations.add_row(site=site.id,
derived_state=mutation.derived_state,
node=node_map[mutation.node])
msprime.sort_tables(nodes=new_nodes,
edges=new_edges,
sites=new_sites,
mutations=new_mutations)
provenances = ts.dump_tables().provenances
add_provenance(provenances, "permute_nodes")
return msprime.load_tables(nodes=new_nodes,
edges=new_edges,
sites=new_sites,
mutations=new_mutations,
provenances=provenances)
def insert_branch_sites(ts):
"""
Returns a copy of the specified tree sequence with a site on every branch
of every tree.
"""
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
for tree in ts.trees():
left, right = tree.interval
delta = (right - left) / len(list(tree.nodes()))
x = left
for u in tree.nodes():
if tree.parent(u) != msprime.NULL_NODE:
site = sites.add_row(position=x, ancestral_state='0')
mutations.add_row(site=site, node=u, derived_state='1')
x += delta
tables = ts.tables
add_provenance(tables.provenances, "insert_branch_sites")
return msprime.load_tables(
nodes=tables.nodes, edges=tables.edges, sites=sites, mutations=mutations,
provenances=tables.provenances)
def test_mutations(self):
nodes = msprime.NodeTable()
edges = msprime.EdgeTable()
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
metadata = ExampleMetadata(one="node1", two="node2")
pickled = pickle.dumps(metadata)
nodes.add_row(time=0)
sites.add_row(position=0.1, ancestral_state="A")
mutations.add_row(site=0, node=0, derived_state="T", metadata=pickled)
ts = msprime.load_tables(
nodes=nodes, edges=edges, sites=sites, mutations=mutations,
sequence_length=1)
mutation = ts.site(0).mutations[0]
self.assertEqual(mutation.site, 0)
self.assertEqual(mutation.node, 0)
self.assertEqual(mutation.derived_state, "T")
self.assertEqual(mutation.metadata, pickled)
unpickled = pickle.loads(mutation.metadata)
self.assertEqual(unpickled.one, metadata.one)
self.assertEqual(unpickled.two, metadata.two)
def ts_private_mutations_only(ts):
"""
Returns a new tree sequence which is a single tree and contains at least
one singleton for each sample.
"""
ll_tables = ts.dump_tables().asdict()
mt = msprime.MutationTable()
st = msprime.SiteTable()
positions = sorted([np.random.random() for _ in range(ts.num_samples)])
for i, n in enumerate(ts.samples()):
st.add_row(positions[i], '0')
mt.add_row(i, n, '1')
ll_tables['sites'] = st.asdict()
ll_tables['mutations'] = mt.asdict()
ts_singletons = msprime.tskit.tables.TableCollection.fromdict(
ll_tables).tree_sequence()
return ts_singletons
def write_vcf(chrom):
treefile = args.tree_file[chrom]
vcf = open(args.vcffile[chrom], "w")
mut_rate = args.mut_rate[chrom]
seed = seeds[chrom]
logfile.write("Simulating mutations on" + treefile + "\n")
ts = msprime.load(treefile)
rng = msprime.RandomGenerator(seed)
nodes = msprime.NodeTable()
edgesets = msprime.EdgesetTable()
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
migrations = msprime.MigrationTable()
ts.dump_tables(nodes=nodes, edgesets=edgesets, migrations=migrations)
mutgen = msprime.MutationGenerator(rng, mut_rate)
mutgen.generate(nodes, edgesets, sites, mutations)
logfile.write("Saving to" + args.vcffile[chrom] + "\n")
mutated_ts = msprime.load_tables(nodes=nodes,
edgesets=edgesets,
sites=sites,
mutations=mutations)
mutated_ts.write_vcf(vcf, ploidy=1)
return True
def get_tree_sequence(self, rescale_positions=True, all_sites=False):
"""
Returns the current state of the build tree sequence. All samples and
ancestors will have the sample node flag set.
"""
# TODO Change the API here to ask whether we want a final tree sequence
# or not. In the latter case we also need to translate the ancestral
# and derived states to the input values.
tsb = self.tree_sequence_builder
flags, time = tsb.dump_nodes()
nodes = msprime.NodeTable()
nodes.set_columns(flags=flags, time=time)
left, right, parent, child = tsb.dump_edges()
if rescale_positions:
position = self.sample_data.position[:]
sequence_length = self.sample_data.sequence_length
if sequence_length is None or sequence_length < position[-1]:
sequence_length = position[-1] + 1
# Subset down to the variants.
position = position[self.sample_data.variant_site[:]]
x = np.hstack([position, [sequence_length]])
x[0] = 0
left = x[left]
right = x[right]
else:
position = np.arange(tsb.num_sites)
sequence_length = max(1, tsb.num_sites)
edges = msprime.EdgeTable()
edges.set_columns(left=left, right=right, parent=parent, child=child)
sites = msprime.SiteTable()
sites.set_columns(
position=position,
ancestral_state=np.zeros(tsb.num_sites, dtype=np.int8) + ord('0'),
ancestral_state_offset=np.arange(tsb.num_sites + 1,
dtype=np.uint32))
mutations = msprime.MutationTable()
site = np.zeros(tsb.num_mutations, dtype=np.int32)
node = np.zeros(tsb.num_mutations, dtype=np.int32)
parent = np.zeros(tsb.num_mutations, dtype=np.int32)
derived_state = np.zeros(tsb.num_mutations, dtype=np.int8)
site, node, derived_state, parent = tsb.dump_mutations()
derived_state += ord('0')
mutations.set_columns(site=site,
node=node,
derived_state=derived_state,
derived_state_offset=np.arange(
tsb.num_mutations + 1, dtype=np.uint32),
parent=parent)
if all_sites:
# Append the sites and mutations for each singleton.
num_singletons = self.sample_data.num_singleton_sites
singleton_site = self.sample_data.singleton_site[:]
singleton_sample = self.sample_data.singleton_sample[:]
pos = self.sample_data.position[:]
new_sites = np.arange(len(sites),
len(sites) + num_singletons,
dtype=np.int32)
sites.append_columns(
position=pos[singleton_site],
ancestral_state=np.zeros(num_singletons, dtype=np.int8) +
ord('0'),
ancestral_state_offset=np.arange(num_singletons + 1,
dtype=np.uint32))
mutations.append_columns(
site=new_sites,
node=self.sample_ids[singleton_sample],
derived_state=np.zeros(num_singletons, dtype=np.int8) +
ord('1'),
derived_state_offset=np.arange(num_singletons + 1,
dtype=np.uint32))
# Get the invariant sites
num_invariants = self.sample_data.num_invariant_sites
invariant_site = self.sample_data.invariant_site[:]
sites.append_columns(
position=pos[invariant_site],
ancestral_state=np.zeros(num_invariants, dtype=np.int8) +
ord('0'),
ancestral_state_offset=np.arange(num_invariants + 1,
dtype=np.uint32))
msprime.sort_tables(nodes, edges, sites=sites, mutations=mutations)
return msprime.load_tables(nodes=nodes,
edges=edges,
sites=sites,
mutations=mutations,
sequence_length=sequence_length)
def _load_legacy_hdf5_v2(root, remove_duplicate_positions):
# Get the coalescence records
trees_group = root["trees"]
old_timestamp = datetime.datetime.min.isoformat()
provenances = msprime.ProvenanceTable()
provenances.add_row(timestamp=old_timestamp,
record=_get_v2_provenance("generate_trees",
trees_group.attrs))
num_rows = trees_group["node"].shape[0]
index = np.arange(num_rows, dtype=int)
parent = np.zeros(2 * num_rows, dtype=np.int32)
parent[2 * index] = trees_group["node"]
parent[2 * index + 1] = trees_group["node"]
left = np.zeros(2 * num_rows, dtype=np.float64)
left[2 * index] = trees_group["left"]
left[2 * index + 1] = trees_group["left"]
right = np.zeros(2 * num_rows, dtype=np.float64)
right[2 * index] = trees_group["right"]
right[2 * index + 1] = trees_group["right"]
child = np.array(trees_group["children"], dtype=np.int32).flatten()
edges = msprime.EdgeTable()
edges.set_columns(left=left, right=right, parent=parent, child=child)
cr_node = np.array(trees_group["node"], dtype=np.int32)
num_nodes = max(np.max(child), np.max(cr_node)) + 1
sample_size = np.min(cr_node)
flags = np.zeros(num_nodes, dtype=np.uint32)
population = np.zeros(num_nodes, dtype=np.int32)
time = np.zeros(num_nodes, dtype=np.float64)
flags[:sample_size] = msprime.NODE_IS_SAMPLE
cr_population = np.array(trees_group["population"], dtype=np.int32)
cr_time = np.array(trees_group["time"])
time[cr_node] = cr_time
population[cr_node] = cr_population
if "samples" in root:
samples_group = root["samples"]
population[:sample_size] = samples_group["population"]
if "time" in samples_group:
time[:sample_size] = samples_group["time"]
nodes = msprime.NodeTable()
nodes.set_columns(flags=flags, population=population, time=time)
sites = msprime.SiteTable()
mutations = msprime.MutationTable()
if "mutations" in root:
mutations_group = root["mutations"]
_convert_hdf5_mutations(mutations_group, sites, mutations,
remove_duplicate_positions)
provenances.add_row(timestamp=old_timestamp,
record=_get_v2_provenance("generate_mutations",
mutations_group.attrs))
provenances.add_row(_get_upgrade_provenance(root))
msprime.sort_tables(nodes=nodes,
edges=edges,
sites=sites,
mutations=mutations)
return msprime.load_tables(nodes=nodes,
edges=edges,
sites=sites,
mutations=mutations,
provenances=provenances)
population=nodes['population'],
time=nodes['generation'])
es = msprime.EdgeTable()
es.set_columns(left=edges['left'],
right=edges['right'],
parent=edges['parent'],
child=edges['child'])
st = msprime.SiteTable()
st.set_columns(position=mutas['position'],
ancestral_state=np.zeros(len(mutas['position']), np.int8),
ancestral_state_length=np.ones(len(mutas['position']),
np.uint32))
mt = msprime.MutationTable()
mt.set_columns(site=np.arange(len(mutas['node_id']), dtype=np.int32),
node=mutas['node_id'],
derived_state=np.ones(len(mutas['node_id']), np.int8),
derived_state_length=np.ones(len(mutas['node_id']),
np.uint32))
# Sort
msprime.sort_tables(nodes=nt, edges=es, sites=st, mutations=mt)
print("num total mutations: ", st.num_rows)
# Simplify: this is where the magic happens
## PLR: since these tables aren't valid, you gotta use simplify_tables, not load them into a tree sequence
nt_c = nt.copy()
es_c = es.copy()
st_c = st.copy()
def _load_legacy_hdf5_v10(root, remove_duplicate_positions=False):
# We cannot have duplicate positions in v10, so this parameter is ignored
nodes_group = root["nodes"]
nodes = msprime.NodeTable()
metadata = None
metadata_offset = None
if "metadata" in nodes_group:
metadata = nodes_group["metadata"]
metadata_offset = nodes_group["metadata_offset"]
nodes.set_columns(flags=nodes_group["flags"],
population=nodes_group["population"],
time=nodes_group["time"],
metadata=metadata,
metadata_offset=metadata_offset)
edges_group = root["edges"]
edges = msprime.EdgeTable()
edges.set_columns(left=edges_group["left"],
right=edges_group["right"],
parent=edges_group["parent"],
child=edges_group["child"])
migrations_group = root["migrations"]
migrations = msprime.MigrationTable()
if "left" in migrations_group:
migrations.set_columns(left=migrations_group["left"],
right=migrations_group["right"],
node=migrations_group["node"],
source=migrations_group["source"],
dest=migrations_group["dest"],
time=migrations_group["time"])
sites_group = root["sites"]
sites = msprime.SiteTable()
if "position" in sites_group:
metadata = None
metadata_offset = None
if "metadata" in sites_group:
metadata = sites_group["metadata"]
metadata_offset = sites_group["metadata_offset"]
sites.set_columns(
position=sites_group["position"],
ancestral_state=sites_group["ancestral_state"],
ancestral_state_offset=sites_group["ancestral_state_offset"],
metadata=metadata,
metadata_offset=metadata_offset)
mutations_group = root["mutations"]
mutations = msprime.MutationTable()
if "site" in mutations_group:
metadata = None
metadata_offset = None
if "metadata" in mutations_group:
metadata = mutations_group["metadata"]
metadata_offset = mutations_group["metadata_offset"]
mutations.set_columns(
site=mutations_group["site"],
node=mutations_group["node"],
parent=mutations_group["parent"],
derived_state=mutations_group["derived_state"],
derived_state_offset=mutations_group["derived_state_offset"],
metadata=metadata,
metadata_offset=metadata_offset)
provenances_group = root["provenances"]
provenances = msprime.ProvenanceTable()
if "timestamp" in provenances_group:
timestamp = provenances_group["timestamp"]
timestamp_offset = provenances_group["timestamp_offset"]
if "record" in provenances_group:
record = provenances_group["record"]
record_offset = provenances_group["record_offset"]
else:
record = np.empty_like(timestamp)
record_offset = np.zeros_like(timestamp_offset)
provenances.set_columns(timestamp=timestamp,
timestamp_offset=timestamp_offset,
record=record,
record_offset=record_offset)
provenances.add_row(_get_upgrade_provenance(root))
return msprime.load_tables(nodes=nodes,
edges=edges,
migrations=migrations,
sites=sites,
mutations=mutations,
provenances=provenances)
