def test_DumpConns(self):
"""Test dumping connections."""
ldict = {
'elements': 'iaf_psc_alpha',
'rows': 3,
'columns': 3,
'extent': [2., 2.],
'edge_wrap': True
}
cdict = {
'connection_type': 'divergent',
'mask': {
'circular': {
'radius': 1.
}
}
}
nest.ResetKernel()
l = topo.CreateLayer(ldict)
topo.ConnectLayers(l, l, cdict)
topo.DumpLayerConnections(
l, 'static_synapse',
os.path.join(self.nest_tmpdir(), 'test_DumpConns.out.cnn'))
self.assertTrue(True)
def plot_connections(layer_dict, conn_dict, file_name):
conn_dict['synapse_model'] = 'static_synapse'
layer = tp.CreateLayer(layer_dict)
tp.ConnectLayers(layer, layer, conn_dict)
fig = tp.PlotLayer(layer)
tp.PlotTargets(tp.FindCenterElement(layer),
layer,
fig=fig,
tgt_color='red')
pylab.savefig(file_name)
#pylab.show()
tp.DumpLayerConnections(layer, conn_dict['synapse_model'],
file_name + ".dump")
positions = tp.GetTargetPositions(layer, layer)
'edge_wrap': True
})
topo.ConnectLayers(
l1, l2, {
'connection_type': direction,
'mask': {
'circular': {
'radius': 0.4
}
},
'weights': {
'linear': {
'c': 1.,
'a': -5.
}
}
})
topo.DumpLayerNodes(l1 + l2, 'topo_mpi_test.lyr_tmp')
topo.DumpLayerConnections(l1, 'static_synapse', 'topo_mpi_test.cnn_tmp')
# combine all layer and connection files into one sorted file, respectively
nest.sli_run('SyncProcesses') # make sure all are done dumping
if nest.Rank() == 0:
for filetype in ['cnn', 'lyr']:
os.system('cat *.{0}_tmp | sort > all_sorted.{0}'.format(filetype))
os.system('rm *.{0}_tmp'.format(filetype))
# directories for any number of MPI processes should now be diff-able
def create_spike_times(Sim, NModel):
if Sim.overwrite_files == "no":
if Sim.data_folder.is_dir()==True:
print("You are not allowed to overwrite the files.")
sys.exit("Error message")
else:
Sim.data_folder.mkdir(parents=True)
elif Sim.overwrite_files == "yes":
if Sim.data_folder.is_dir()==True:
print("You have overwritten old files.")
else:
Sim.data_folder.mkdir(parents=True)
else:
print("You have two options for overwrite files")
connections_path = Sim.data_folder / "connections.txt"
spikes_path = Sim.data_folder / "spikes.mat"
spikes_split_path = Sim.data_folder / "spikes_split.mat"
multimeter_data_path = Sim.data_folder / "multimeter_data.mat"
hyperparameters_path = Sim.data_folder / "hyperparameters.mat"
position_path = Sim.data_folder / "position.mat"
# Define important simulation parameters
nest.ResetKernel()
seed=1008.0
nest.SetKernelStatus({"resolution": Sim.resolution,
"print_time": True,
"overwrite_files":True,
"grng_seed": int(seed),
"rng_seeds": [int(seed)]
})
# Construct the position grid of the neural network(NN)
jit = 0.03
if NModel.nr_neurons==100:
xs = np.arange(-0.45,.451,0.1) # defines the size of the network
elif NModel.nr_neurons==16:
xs = np.arange(-0.15,.151,0.1)
else:
print("Current network can constitute of 16 or 100 neurons.")
np.random.seed(int(seed))
pos = [[x,y] for y in xs for x in xs]
pos = [[p[0]+np.random.uniform(-jit,jit),p[1]+np.random.uniform(-jit,jit)] for p in pos]
# Construct the neurons on the grid and establish connections between them.
# The probabilty of connection varies with the distance between the neurons
# Define synapse connections
nest.SetDefaults("tsodyks_synapse",{"delay": NModel.t_delay, #1.5 in Stetter's code
"tau_rec": NModel.tau_rec,
"tau_fac":0.0,
"U": NModel.U
})
conn1 = { "connection_type":"divergent",
"mask": {"circular":{"radius":0.75}},
"kernel": {"gaussian":{"p_center":1.,"sigma":0.15}}, #0.15 for 100 neurons
"allow_autapses":False,
"synapse_model":"tsodyks_synapse",
"weights": NModel.alpha_int
}
# specify the neural model
neuron_param= {
#"I_e" : 0.0,
"C_m" : 1.0,
"tau_m" : NModel.tau_m,
"t_ref" : NModel.tau_s, #refactory periods in ms 2.0 is default
"E_L" : 0.0,
"V_th" : NModel.V_thres,
"V_m" : 0.0,
"V_reset" : 0.0
}
nest.SetDefaults("iaf_psc_alpha", neuron_param)
layer_dict_ex = {"positions": pos,
"extent" : [1.1,1.1],
"elements" : "iaf_psc_alpha"}
layer = topp.CreateLayer(layer_dict_ex)
topp.ConnectLayers(layer,layer,conn1)
# Plot layer
topp.PlotLayer(layer)
# change the seed for different Poisson spike trains
nest.SetKernelStatus({
'grng_seed': int(seed),
'rng_seeds': [int(seed)]
})
# Creation of a poisson generator
nest.CopyModel('poisson_generator', 'PG',
params={'rate': NModel.poisson_spike_rate}) #1.6 in the paper, I don't know why they changed it in the programm
pg = topp.CreateLayer({ 'rows' : 1,
'columns' : 1,
'elements' : 'PG'})
cdict_stim = {'connection_type' : 'divergent',
'weights': NModel.alpha_ext}
topp.ConnectLayers(pg,layer,cdict_stim)
# create multimeter
nrns=nest.GetLeaves(layer,local_only=True)[0]
multimeter = nest.Create("multimeter", NModel.nr_neurons)
nest.SetStatus(multimeter, {"withtime":True, "record_from":["V_m","I_syn_ex"],"interval":Sim.interval}) #, "input_currents_ex","input_currents_in"
nest.Connect(multimeter,nrns,"one_to_one")
# Create spike detector
sd1 = nest.Create('spike_detector')
nest.SetStatus(sd1,{'precise_times':True})
nest.Connect(nrns,sd1)
# Simulate
nest.Simulate(Sim.sim_length + Sim.interval)
# Retrieve the generated data
[potential,currents_ex,spikes,time]=get_data(multimeter,sd1,NModel.nr_neurons,Sim.nr_samples)
# Save the spikes to a file
spikesdict={'N1':spikes}
sio.savemat(str(spikes_path),spikesdict)
if Sim.P>1:
potential_fin = time_series_split(potential,Sim.P)[0]
currents_ex_fin = time_series_split(currents_ex,Sim.P)[0]
spikesdict_split = spike_times_split(spikes, Sim.sim_length, Sim.P)
else:
potential_fin=potential
currents_ex_fin=currents_ex
spikesdict_split = {'N1':spikes}
# Pass important hyperparameters to create_fluorescent_data_from_spike_times.py
hyperdict = {
'P' : Sim.P,
'length_ts' : Sim.length_ts,
'nr_samples' : Sim.nr_samples,
'nr_neurons' : NModel.nr_neurons,
'interval' : Sim.interval
}
sio.savemat(str(hyperparameters_path),hyperdict)
# save the position
sio.savemat(str(position_path), {'position' : pos})
# save the results
sio.savemat(str(multimeter_data_path),mdict={ 'potential' : potential_fin,
'input_currents_ex': currents_ex_fin
})
sio.savemat(str(spikes_split_path),spikesdict_split)
topp.DumpLayerConnections(layer,'tsodyks_synapse',str(connections_path))
def simulation(Params):
#! =================
#! Import network
#! =================
# NEST Kernel and Network settings
nest.ResetKernel()
nest.ResetNetwork()
nest.SetKernelStatus({"local_num_threads": Params['threads'],'resolution': Params['resolution']})
nest.SetStatus([0],{'print_time': True})
# initialize random seed
import time
msd = int(round(time.time() * 1000))
nest.SetKernelStatus({'grng_seed' : msd})
nest.SetKernelStatus({'rng_seeds' : range(msd+Params['threads']+1, msd+2*Params['threads']+1)})
import importlib
network = importlib.import_module(Params['network'])
reload(network)
models, layers, conns = network.get_Network(Params)
#import network_full_keiko
#reload(network_full_keiko)
# models, layers, conns = network_full_keiko.get_Network(Params)
# Create models
for m in models:
nest.CopyModel(m[0], m[1], m[2])
# Create layers, store layer info in Python variable
for l in layers:
exec '%s = tp.CreateLayer(l[1])' % l[0]
# Create connections, need to insert variable names
for c in conns:
eval('tp.ConnectLayers(%s,%s,c[2])' % (c[0], c[1]))
# Prepare for file IO
import glob
# --- Set folder information
data_folder = Params['data_folder']
if not os.path.isdir(data_folder):
os.makedirs(data_folder)
# --- To save spike data, set pairs of population id and its name
population_name = [ {'population': Retina_layer, 'name': 'Retina'},
{'population': Vp_vertical, 'name': 'Vp_v'},
{'population': Vp_horizontal, 'name': 'Vp_h'},
{'population': Rp_layer, 'name': 'Rp'},
{'population': Tp_layer, 'name': 'Tp'},
{'population': Vs_vertical, 'name': 'Vs_v'},
{'population': Vs_horizontal, 'name': 'Vs_h'}]
if Params.has_key('load_connections_from_file') and Params['load_connections_from_file']:
# Preparation
scramble_populations = [(Vp_vertical, 'Vp_vertical'),
(Vp_horizontal, 'Vp_horizontal')]
scramble_layers = ['L23_exc',
'L23_inh',
'L4_exc',
'L4_inh',
'L56_exc',
'L56_inh']
#scramble_layers = ['L4_exc']
# Get min &max index of each layer
h_min_idx = {}
h_max_idx = {}
v_min_idx = {}
v_max_idx = {}
target_neurons = []
for tmp_model in scramble_layers:
tmp_h = nest.GetLeaves(Vp_horizontal, properties={'model': tmp_model}, local_only=True)[0]
tmp_v = nest.GetLeaves(Vp_vertical, properties={'model': tmp_model}, local_only=True)[0]
h_min_idx[tmp_model] = min(tmp_h)
h_max_idx[tmp_model] = max(tmp_h)
v_min_idx[tmp_model] = min(tmp_v)
v_max_idx[tmp_model] = max(tmp_v)
target_neurons = target_neurons + range(h_min_idx[tmp_model], h_max_idx[tmp_model]+1) + range(v_min_idx[tmp_model], v_max_idx[tmp_model]+1)
# Save intact network information
for p in range(0, len(population_name), 1):
population = population_name[p]['population']
p_name = population_name[p]['name']
filename_AMPA = data_folder + 'connection_' + p_name + '_AMPA_syn' + '_intact.dat'
filename_NMDA = data_folder + 'connection_' + p_name + '_NMDA_syn' + '_intact.dat'
filename_GABAA = data_folder + 'connection_' + p_name + '_GABA_A_syn' + '_intact.dat'
filename_GABAB = data_folder + 'connection_' + p_name + '_GABA_B_syn' + '_intact.dat'
tp.DumpLayerConnections(population, 'AMPA_syn', filename_AMPA)
tp.DumpLayerConnections(population, 'NMDA_syn', filename_NMDA)
tp.DumpLayerConnections(population, 'GABA_A_syn', filename_GABAA)
tp.DumpLayerConnections(population, 'GABA_B_syn', filename_GABAB)
# Reset network
nest.ResetNetwork()
'''
# The following code works, but takes time longer
# Get the list of connection_file
file_list = glob.glob(data_folder+'connection_*_intact.dat')
# Remove a file if the size is zero
remove_idx = []
for file_idx in range(0,len(file_list)):
intact_filename = file_list[file_idx]
fsize=os.path.getsize(intact_filename)
if fsize == 0:
remove_idx = remove_idx + [file_idx]
remove_idx.sort()
remove_idx.reverse()
for i in remove_idx:
del file_list[i]
'''
# TODO : put GABA_A, GABA_B and NMDA connection files
file_list = []
file_list.append({'filename': data_folder + 'connection_Vp_h_AMPA_syn_intact.dat',
'synapse': 'AMPA'})
file_list.append({'filename': data_folder + 'connection_Vp_v_AMPA_syn_intact.dat',
'synapse': 'AMPA'})
# Do the following procedure for all connection files
for file_idx in range(0,len(file_list)):
# Set filenames
intact_filename = file_list[file_idx]['filename']
receptors = nest.GetDefaults('ht_neuron')['receptor_types']
syn_model = receptors[ file_list[file_idx]['synapse'] ]
scrambled_filename = intact_filename.replace('intact', 'scrambled')
print(intact_filename)
# Get original(intact) connectivity data
src_network = np.loadtxt(open(intact_filename,'rb'))
np_pre = src_network[:, 0].astype(int)
np_post = src_network[:, 1].astype(int)
np_w = src_network[:, 2]
np_d = src_network[:, 3]
if Params['scrambled']:
# Preserve the original structure if
# -- pre neruons are not in target populations (i.e. scramble_layers)
# -- OR
# -- post neurons are not in target populations(i.e. scramble_layers)
preserved_rows = np.where( ~np.in1d(np_pre,target_neurons) | ~np.in1d(np_post,target_neurons) )[0]
preserved_pre = np_pre[preserved_rows]
preserved_post = np_post[preserved_rows]
preserved_w = np_w[preserved_rows]
preserved_d = np_d[preserved_rows]
# If len(preserved_rows)==len(np_pre), that means all neurons do not belong to scramble target areas.
# If len(preserved_rows) < len(np_pre), that means there are some neurons which need to be scrambled.
if len(preserved_rows) > len(np_pre):
print('ERROR: preserved_rows should not be larger than np_pre')
elif len(preserved_rows) == len(np_pre):
scrambled_pre = preserved_pre.tolist()
scrambled_post = preserved_post.tolist()
scrambled_w = preserved_w.tolist()
scrambled_d = preserved_d.tolist()
else: # --- len(preserved_rows) < len(np_pre)
scrambled_pre = []
scrambled_post = []
scrambled_w = []
scrambled_d = []
for tmp_model_pre in scramble_layers:
for tmp_model_post in scramble_layers:
# Get row index such that
# --- pre_neuron is in "tmp_model_pre"
# --- AND
# --- post_neuron is in "tmp_model_post"
bool_pre_h = np.in1d(np_pre, range(h_min_idx[tmp_model_pre], h_max_idx[tmp_model_pre]+1))
bool_pre_v = np.in1d(np_pre, range(v_min_idx[tmp_model_pre], v_max_idx[tmp_model_pre]+1))
bool_post_h = np.in1d(np_post, range(h_min_idx[tmp_model_post], h_max_idx[tmp_model_post]+1))
bool_post_v = np.in1d(np_post, range(v_min_idx[tmp_model_post], v_max_idx[tmp_model_post]+1))
tmp_rows_pre_h = np.where(bool_pre_h & (bool_post_h|bool_post_v))[0]
tmp_rows_pre_v = np.where(bool_pre_v & (bool_post_h|bool_post_v))[0]
# Get connectivity data such that pre neuron is in "tmp_model_pre"
# --- pre, w and d information should be kept.
# --- post index should be scrambled.
tmp_pre = np_pre[np.append(tmp_rows_pre_h, tmp_rows_pre_v)].tolist()
tmp_w = np_w[np.append(tmp_rows_pre_h, tmp_rows_pre_v)].tolist()
tmp_d = np_d[np.append(tmp_rows_pre_h, tmp_rows_pre_v)].tolist()
tmp_post = []
num_pre_h = len(tmp_rows_pre_h)
num_pre_v = len(tmp_rows_pre_v)
# --- pre : population = horizontal, model = "tmp_model_pre"
# --- post: population = horizontal(1/2)+vertical(1/2), model = "tmp_model_post"
if num_pre_h > 0:
# Assign the same number of connections for horizontal population and vertical population
num_scrambled_h = int(round(num_pre_h / 2))
num_scrambled_v = num_pre_h - num_scrambled_h
# Choose post neuron index randomly
scrambled_h_idx = rd.randint(low =h_min_idx[tmp_model_post],
high=h_max_idx[tmp_model_post],
size=[num_scrambled_h, 1])
scrambled_v_idx = rd.randint(low =v_min_idx[tmp_model_post],
high=v_max_idx[tmp_model_post],
size=[num_scrambled_v, 1])
# append scrambled post index
tmp_post = tmp_post + np.append(scrambled_h_idx, scrambled_v_idx).tolist()
# --- pre : population = vertical, model = "tmp_model_pre"
# --- post: population = horizontal(1/2)+vertical(1/2), model = "tmp_model_post"
if num_pre_v > 0:
# Assign the same number of connections for horizontal population and vertical population
num_scrambled_h = int(round(num_pre_v / 2))
num_scrambled_v = num_pre_v - num_scrambled_h
# Choose post neuron index randomly
scrambled_h_idx = rd.randint(low =h_min_idx[tmp_model_post],
high=h_max_idx[tmp_model_post],
size=[num_scrambled_h, 1])
scrambled_v_idx = rd.randint(low =v_min_idx[tmp_model_post],
high=v_max_idx[tmp_model_post],
size=[num_scrambled_v, 1])
# append scrambled post index
tmp_post = tmp_post + np.append(scrambled_h_idx, scrambled_v_idx).tolist()
scrambled_pre = scrambled_pre + tmp_pre
scrambled_post = scrambled_post + tmp_post
scrambled_w = scrambled_w + tmp_w
scrambled_d = scrambled_d +tmp_d
# append preserved connection data
scrambled_pre = scrambled_pre + preserved_pre.tolist()
scrambled_post = scrambled_post + preserved_post.tolist()
scrambled_w = scrambled_w + preserved_w.tolist()
scrambled_d = scrambled_d + preserved_d.tolist()
# Save scrambled_data
scrambled_all_data = np.zeros([len(scrambled_pre), 4])
scrambled_all_data[:, 0] = scrambled_pre
scrambled_all_data[:, 1] = scrambled_post
scrambled_all_data[:, 2] = scrambled_w
scrambled_all_data[:, 3] = scrambled_d
np.savetxt(scrambled_filename, scrambled_all_data, fmt='%.6f')
# Connect
con_dict = {'rule': 'one_to_one'}
syn_dict = {"model": "ht_synapse",
'receptor_type': syn_model,
'weight': scrambled_w,
'delay': scrambled_d,
}
nest.Connect(scrambled_pre, scrambled_post, con_dict, syn_dict)
else:
# just convert data type(ndarray->list) and connect based on the original data
pre = np_pre.tolist()
post = np_post.tolist()
w = np_w.tolist()
d = np_d.tolist()
# Connect
con_dict = {'rule': 'one_to_one'}
syn_dict = {"model": "ht_synapse",
'receptor_type': 1,
'weight': w,
'delay': d,
}
nest.Connect(pre, post, con_dict, syn_dict)
# nest.DisconnectOneToOne(tp_node, tgt_map[0], {"synapse_model": "AMPA_syn"})
#nest.Disconnect([tp_node], tgt_map, 'one_to_one', {"synapse_model": "AMPA_syn"})
# Get target nodes for the vertical population
# tp_nodes = nest.GetLeaves(Tp_layer, local_only=True)[0]
if Params.has_key('show_V4_num_conn_figure') and Params['show_V4_num_conn_figure']:
horizontal_nodes = nest.GetLeaves(Vp_horizontal, properties={'model': 'L4_exc'}, local_only=True)[0]
vertical_nodes = nest.GetLeaves(Vp_vertical, properties={'model': 'L4_exc'}, local_only=True)[0]
n_conns_hor = []
for (idx, horizontal_node) in enumerate(horizontal_nodes):
tgt_map = []
this_conns = nest.GetConnections([horizontal_node], horizontal_nodes, synapse_model='AMPA_syn')
tgt_map.extend([conn[1] for conn in this_conns])
n_conns_hor.append(len(tgt_map))
plt.figure()
plt.hist(n_conns_hor, range(0, max(n_conns_hor + [30])))
plt.title('# of connections Vp(h) L4pyr -> Vp(h) L4Pyr')
n_conns_hor = []
for (idx, horizontal_node) in enumerate(horizontal_nodes):
tgt_map = []
this_conns = nest.GetConnections([horizontal_node], vertical_nodes, synapse_model='AMPA_syn')
tgt_map.extend([conn[1] for conn in this_conns])
n_conns_hor.append(len(tgt_map))
# nest.DisconnectOneToOne(tp_node, tgt_map[0], {"synapse_model": "AMPA_syn"})
#nest.Disconnect([tp_node], tgt_map, 'one_to_one', {"synapse_model": "AMPA_syn"})
plt.figure()
plt.hist(n_conns_hor, range(0, max(n_conns_hor + [30])))
plt.title('# of connections Vp(h) L4pyr -> Vp(v) L4Pyr')
n_conns_ver = []
for (idx, vertical_node) in enumerate(vertical_nodes):
tgt_map = []
this_conns = nest.GetConnections([vertical_node], vertical_nodes, synapse_model='AMPA_syn')
tgt_map.extend([conn[1] for conn in this_conns])
n_conns_ver.append(len(tgt_map))
plt.figure()
plt.hist(n_conns_ver, range(0, max(n_conns_ver + [30])))
plt.title('# of connections Vp(v) L4pyr -> Vp(v) L4Pyr')
n_conns_ver = []
for (idx, vertical_node) in enumerate(vertical_nodes):
tgt_map = []
this_conns = nest.GetConnections([vertical_node], horizontal_nodes, synapse_model='AMPA_syn')
tgt_map.extend([conn[1] for conn in this_conns])
n_conns_ver.append(len(tgt_map))
plt.figure()
plt.hist(n_conns_ver, range(0, max(n_conns_ver + [30])))
plt.title('# of connections Vp(v) L4pyr -> Vp(h) L4Pyr')
plt.show()
# Check connections
# Connections from Retina to TpRelay
# tp.PlotTargets(tp.FindCenterElement(Retina_layer), Tp_layer)
if Params.has_key('show_V4_connectivity_figure') and Params['show_V4_connectivity_figure']:
Vp_hor_gids = tp.GetElement(Vp_horizontal, [0,0])
n_Vp_hor = len(Vp_hor_gids)
f = []
for idx in range(n_Vp_hor):
f.append(plt.figure())
positions = range(0,41,10)
positions[-1] -= 1
for xi in range(len(positions)):
for yi in range(len(positions)):
print("Position [%d,%d] : %d" %(xi,yi,yi*(len(positions))+xi+1))
x = positions[xi]
y = positions[yi]
Vp_hor_gids = tp.GetElement(Vp_horizontal, [x,y])
Vp_hor_status = nest.GetStatus(Vp_hor_gids)
for (idx, n) in enumerate(Vp_hor_status):
if n['Tau_theta'] == 2.0:
print idx
try:
f[idx].add_subplot(len(positions), len(positions), yi*(len(positions))+xi+1)
tp.PlotTargets([Vp_hor_gids[idx]], Vp_horizontal, 'L4_exc', 'AMPA_syn', f[idx])
except:
print('%i bad' % Vp_hor_gids[idx])
plt.show()
# Connections from TpRelay to L4pyr in Vp (horizontally tuned)
#topo.PlotTargets(topo.FindCenterElement(Tp), Vp_h, 'L4pyr', 'AMPA')
#pylab.title('Connections TpRelay -> Vp(h) L4pyr')
#pylab.show()
# Connections from TpRelay to L4pyr in Vp (vertically tuned)
#topo.PlotTargets(topo.FindCenterElement(Tp), Vp_v, 'L4pyr', 'AMPA')
#pylab.title('Connections TpRelay -> Vp(v) L4pyr')
#pylab.show()
'''
# pablo
# Create vertical grating
for n in nest.GetLeaves(Retina_layer)[0]:
retina_0 = (nest.GetLeaves(Retina_layer)[0])[0]
col = (n-retina_0)/Params['Np']
cells_per_degree = Params['Np']/Params['visSize']
cells_per_cycle = cells_per_degree/Params['spatial_frequency']
nest.SetStatus([n], { "phase": col * 360/(cells_per_cycle-1) })
'''
### keiko
if Params['lambda_dg'] >= 0:
[nest.SetStatus([n], {"phase": phaseInit(tp.GetPosition([n])[0],
Params["lambda_dg"],
Params["phi_dg"])})
for n in nest.GetLeaves(Retina_layer)[0]]
else:
# Leonardo: Random retina input
[nest.SetStatus([n], {"phase": phaseInit(tp.GetPosition([n])[0],
np.pi * np.random.rand(),
np.pi * np.random.rand())})
for n in nest.GetLeaves(Retina_layer)[0]]
# --------------------------------------------------------------------#
# ---------- SET IB NEURONS ----------------------------------------- #
# --------------------------------------------------------------------#
# 30% of Cortex L56 excitatory neurons are intrinsically bursting(IB) neuron.
# That is achieved by setting pacemaker current I_h.
# So select 30% of L56_exc neuron, and change h_g_peak from 0.0 to 1.0.
# (Other cortical neuron do not have I_h, thus h_g_peak=0.0)
L56_vertical_idx = [nd for nd in nest.GetLeaves(Vp_vertical)[0] if nest.GetStatus([nd], 'model')[0]=='L56_exc']
L56_horizontal_idx = [nd for nd in nest.GetLeaves(Vp_horizontal)[0] if nest.GetStatus([nd], 'model')[0]=='L56_exc']
num_neuron = len(L56_vertical_idx)
num_ib = int(num_neuron*0.3)
ridx_vertical = np.random.randint(num_neuron, size=(1,num_ib))[0]
ridx_horizontal = np.random.randint(num_neuron, size=(1,num_ib))[0]
for i in range(1,num_ib,1):
nest.SetStatus([L56_vertical_idx[ridx_vertical[i]]], {'h_g_peak': 1.0})
nest.SetStatus([L56_horizontal_idx[ridx_horizontal[i]]], {'h_g_peak': 1.0})
# initiate network activity
#nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'rate': Params['ret_rate']})
nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'rate': Params['ret_rate']})
nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'amplitude': 0.0})
nest.Simulate(500.0)
#! =================
#! Recording devices
#! =================
nest.CopyModel('multimeter', 'RecordingNode',
params = {'interval' : Params['resolution'],
#'record_from': ['V_m'],
'record_from': ['V_m',
'I_syn_AMPA',
'I_syn_NMDA',
'I_syn_GABA_A',
'I_syn_GABA_B',
'g_AMPA',
'g_NMDA',
'g_GABAA',
'g_GABAB',
'I_NaP',
'I_KNa',
'I_T',
'I_h'],
'record_to' : ['memory'],
'withgid' : True,
'withtime' : True})
recorders = []
'''
for population, model in [(Retina_layer, 'Retina'),
(Tp_layer , 'Tp_exc'),
(Tp_layer , 'Tp_inh'),
(Rp_layer , 'Rp'),
(Vp_vertical, 'L23_exc'),
(Vp_horizontal, 'L23_exc'),
(Vp_vertical, 'L23_inh'),
(Vp_horizontal, 'L23_inh'),
(Vp_vertical, 'L4_exc'),
(Vp_horizontal, 'L4_exc'),
(Vp_vertical, 'L4_inh'),
(Vp_horizontal, 'L4_inh'),
(Vp_vertical, 'L56_exc'),
(Vp_horizontal, 'L56_exc'),
(Vp_vertical, 'L56_inh'),
(Vp_horizontal, 'L56_inh'),
(Vs_vertical, 'L23_exc'),
(Vs_horizontal, 'L23_exc'),
(Vs_vertical, 'L23_inh'),
(Vs_horizontal, 'L23_inh'),
(Vs_vertical, 'L4_exc'),
(Vs_horizontal, 'L4_exc'),
(Vs_vertical, 'L4_inh'),
(Vs_horizontal, 'L4_inh'),
(Vs_vertical, 'L56_exc'),
(Vs_horizontal, 'L56_exc'),
(Vs_vertical, 'L56_inh'),
(Vs_horizontal, 'L56_inh')]:
'''
for population, model in [(Retina_layer, 'Retina'),
(Tp_layer , 'Tp_exc'),
(Tp_layer , 'Tp_inh'),
(Vp_vertical, 'L4_exc'),
(Vp_vertical, 'L4_inh'),
(Vp_horizontal, 'L4_exc'),
(Vp_vertical, 'L23_exc'),
(Vp_horizontal, 'L23_exc'),
(Vp_vertical, 'L56_exc'),
(Rp_layer, 'Rp')]:
rec = nest.Create('RecordingNode')
recorders.append([rec,population,model])
if (model=='Retina'):
nest.SetStatus(rec,{'record_from': ['rate']})
tgts = [nd for nd in nest.GetLeaves(population)[0] if nest.GetStatus([nd], 'model')[0]==model]
nest.Connect(rec, tgts)
#! =================
#! Spike detector
#! =================
detectors = []
'''
for population, model in [(Retina_layer, 'Retina'),
(Tp_layer , 'Tp_exc'),
(Tp_layer , 'Tp_inh'),
(Rp_layer , 'Rp'),
(Vp_vertical, 'L23_exc'),
(Vp_horizontal, 'L23_exc'),
(Vp_vertical, 'L23_inh'),
(Vp_horizontal, 'L23_inh'),
(Vp_vertical, 'L4_exc'),
(Vp_horizontal, 'L4_exc'),
(Vp_vertical, 'L4_inh'),
(Vp_horizontal, 'L4_inh'),
(Vp_vertical, 'L56_exc'),
(Vp_horizontal, 'L56_exc'),
(Vp_vertical, 'L56_inh'),
(Vp_horizontal, 'L56_inh'),
(Vs_vertical, 'L23_exc'),
(Vs_horizontal, 'L23_exc'),
(Vs_vertical, 'L23_inh'),
(Vs_horizontal, 'L23_inh'),
(Vs_vertical, 'L4_exc'),
(Vs_horizontal, 'L4_exc'),
(Vs_vertical, 'L4_inh'),
(Vs_horizontal, 'L4_inh'),
(Vs_vertical, 'L56_exc'),
(Vs_horizontal, 'L56_exc'),
(Vs_vertical, 'L56_inh'),
(Vs_horizontal, 'L56_inh')]:
'''
for population, model in [(Retina_layer, 'Retina'),
(Tp_layer , 'Tp_exc'),
(Tp_layer , 'Tp_inh'),
(Vp_vertical, 'L4_exc'),
(Vp_horizontal, 'L4_exc')]:
rec = nest.Create('spike_detector', params={"withgid": True, "withtime": True})
#rec = nest.Create('spike_detector')
detectors.append([rec,population,model])
tgts = [nd for nd in nest.GetLeaves(population)[0] if nest.GetStatus([nd], 'model')[0]==model]
if model == 'Retina':
for t in tgts:
try:
nest.Connect([t], rec)
print('connected %d' % t)
except:
print('%d did not work' % t)
else:
nest.Connect(tgts, rec)
#! ====================
#! Simulation
#! ====================
'''
# change gKL to 0.8 in all populations (necessary to get a little stronger evoked response)
for l in layers:
sim_elements = l[1]['elements']
for m in np.arange(0,np.size(sim_elements),1):
if(np.size(sim_elements)==1):
sim_model = sim_elements
else:
sim_model = sim_elements[m]
exec("la = %s" % l[0])
pop = [nd for nd in nest.GetLeaves(la)[0] if nest.GetStatus([nd], 'model')[0]==sim_model]
if (l[0]!='Retina_layer'):
for cell in pop:
nest.SetStatus([cell], {'g_KL':0.8})
'''
# Simulate
for t in Params['intervals']:
#if (t == 250.0): # Stimulus ON
# # if (t == 1500.0): # Stimulus ON
# nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'amplitude': 45.0})
#else: # Stimulus OFF
# nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'amplitude': 0.0})
if Params['input_flag']==True:
nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'amplitude': Params['ret_rate']})
else:
nest.SetStatus(nest.GetLeaves(Retina_layer)[0], {'amplitude': 0.0})
nest.Simulate(t)
'''
data_folder = Params['data_folder']
if not os.path.isdir(data_folder):
os.makedirs(data_folder)
'''
#! ====================
#! Plot Results
#! ====================
if Params.has_key('show_main_figure') and Params['show_main_figure']:
print "plotting..."
rows = 9
cols = 2
fig = plt.figure(num=None, figsize=(13, 24), dpi=100)
fig.subplots_adjust(hspace=0.4)
# Plot A: membrane potential rasters
recorded_models = [(Retina_layer,'Retina'),
(Vp_vertical,'L23_exc'),
(Vp_vertical,'L4_exc'),
(Vp_vertical,'L56_exc'),
(Rp_layer,'Rp'),
(Tp_layer,'Tp_exc')]
#plotting.potential_raster(fig,recorders,recorded_models,0,Params['Np'],np.sum(Params['intervals']),Params['resolution'],rows,cols,0)
plotting.potential_raster(fig,recorders,recorded_models,0,Params['Np'],np.sum(Params['intervals']),Params['resolution'],rows,cols,0)
#starting_neuron = 800+1
#plotting.potential_raster(fig,recorders,recorded_models,starting_neuron,Params['Np'],np.sum(Params['intervals']),Params['resolution'],rows,cols,0)
plt.title('Evoked')
# Plot B: individual intracellular traces
recorded_models =[(Vp_vertical,'L4_exc'),
(Vp_vertical,'L4_inh')]
#plotting.intracellular_potentials(fig, recorders, recorded_models, 21, rows, cols, 6) #original
# keiko
total_time = 0.0
for t in Params['intervals']:
total_time += t
#draw_neuron = (Params['Np']*Params['Np']/2)
#plotting.intracellular_potentials(fig, recorders, recorded_models, draw_neuron, rows, cols, 6, total_time)
plotting.intracellular_potentials(fig, recorders, recorded_models, 21, rows, cols, 6, total_time)
#plotting.intracellular_potentials(fig, recorders, recorded_models, 820, rows, cols, 6, total_time)
# Plot C: topographical activity of the vertical and horizontal layers
if Params.has_key('start_membrane_potential') and Params.has_key('end_membrane_potential'):
start = Params['start_membrane_potential']
stop = Params['end_membrane_potential']
else:
start = 130.0
stop = 140.0
recorded_models = [(Vp_vertical,'L23_exc')]
labels = ["Vertical"]
plotting.topographic_representation(fig,
recorders,
recorded_models,
labels,
Params['Np'],
np.sum(Params['intervals']),
Params['resolution'],
rows,
cols,
start,
stop,
8,
0)
recorded_models = [(Vp_horizontal,'L23_exc')]
labels = ["Horizontal"]
plotting.topographic_representation(fig,recorders,recorded_models,labels,Params['Np'],np.sum(Params['intervals']),Params['resolution'],rows,cols,start,stop,8,1)
fig.savefig(data_folder + 'figure3.png', dpi=100)
plt.show()
# Plot D: movie
#labels = ["Evoked_Vp_L23_Vertical","Evoked_Vp_L23_Horizontal"]
#recorded_models = [(Vp_vertical,'L23_exc'),(Vp_horizontal,'L23_exc')]
#plotting.makeMovie(fig,recorders,recorded_models,labels,Params['Np'],np.sum(Params['intervals']),Params['resolution'])
#! ====================
#! Save Results
#! ====================
print('save recorders data')
for rec, population, model in recorders:
# Get name of population
for p in range(0, len(population_name), 1):
if population_name[p]['population'] == population:
p_name = population_name[p]['name']
data = nest.GetStatus(rec)[0]['events']
if model == 'Retina':
scipy.io.savemat(data_folder + '/recorder_' + p_name + '_' + model + '.mat',
mdict={'senders': data['senders'],
'rate': data['rate']})
else:
scipy.io.savemat(data_folder + '/recorder_' + p_name + '_' + model + '.mat',
mdict={'senders': data['senders'],
'V_m': data['V_m'],
'I_syn_AMPA': data['I_syn_AMPA'],
'I_syn_NMDA': data['I_syn_NMDA'],
'I_syn_GABA_A': data['I_syn_GABA_A'],
'I_syn_GABA_B': data['I_syn_GABA_B'],
'g_AMPA': data['g_AMPA'],
'g_NMDA': data['g_NMDA'],
'g_GABAA': data['g_GABAA'],
'g_GABAB': data['g_GABAB']} )
print('save raster images')
plt.close()
for rec, population, model in detectors:
spikes = nest.GetStatus(rec, 'events')[0]
# Get name of population
for p in range(0, len(population_name), 1):
if population_name[p]['population'] == population:
p_name = population_name[p]['name']
if len(nest.GetStatus(rec)[0]['events']['senders']) > 3:
raster = raster_plot.from_device(rec, hist=True)
pylab.title( p_name + '_' + model )
f = raster[0].figure
f.set_size_inches(15, 9)
f.savefig(data_folder + 'spikes_' + p_name + '_' + model + '.png', dpi=100)
plt.close()
# Set filename and save spike data
filename = data_folder + 'spike_' + p_name + '_' + model + '.pickle'
pickle.dump(spikes, open(filename, 'w'))
scipy.io.savemat(data_folder + '/spike_' + p_name + '_' + model + '.mat', mdict={'senders': spikes['senders'], 'times': spikes['times']})
#filename_AMPA = data_folder + 'connection_' + p_name + '_AMPA_syn' + '.dat'
#tp.DumpLayerConnections(population, 'AMPA_syn', filename_AMPA)
'''
filename_AMPA = data_folder + 'connection_' + p_name + '_AMPA_syn' + '.dat'
filename_NMDA = data_folder + 'connection_' + p_name + '_NMDA_syn' + '.dat'
filename_GABAA = data_folder + 'connection_' + p_name + '_GABA_A_syn' + '.dat'
filename_GABAB = data_folder + 'connection_' + p_name + '_GABA_B_syn' + '.dat'
tp.DumpLayerConnections(population, 'AMPA_syn', filename_AMPA)
tp.DumpLayerConnections(population, 'NMDA_syn', filename_NMDA)
tp.DumpLayerConnections(population, 'GABA_A_syn', filename_GABAA)
tp.DumpLayerConnections(population, 'GABA_B_syn', filename_GABAB)
'''
'''
for p in range(0, len(population_name), 1):
population = population_name[p]['population']
p_name = population_name[p]['name']
filename_nodes = data_folder + '/gid_' + p_name + '.dat'
tp.DumpLayerNodes(population, filename_nodes)
'''
network_script = Params['network'] + '.py'
shutil.copy2(network_script, Params['data_folder'] + network_script)
print('end')