dev = u3.U3() # Open LJU3
dev.getCalibrationData()
dt = 100
dev.configIO(EnableCounter1=True, TimerCounterPinOffset=6)
fps = 15.3
top = 10
bottom = 0
steps = 2
n_planes = int(
(top - bottom) / steps) + 1 # total number of planes for each volume
volumes_per_s = fps / n_planes # total number of volume acquisition per volume
pulse_count = 0
with Opto(port='COM6') as etl:
while True:
etl.current(bottom)
pulse_count = int(np.array(dev.getFeedback(u3.Counter(counter=1))))
#print('Reset',pulse_count)
if pulse_count > 0: # for initial trigger (first TTL pulse/first plane of the volume)
print('ETL', etl.current())
print('ini', pulse_count)
#pulse_count -= 1 # reset it to zero so you can image the first plane, if it is one it will immediately increment the focal_plane
old_pulse_count = 0 # to store the previous focal plane
while pulse_count <= n_planes:
if pulse_count > old_pulse_count: # for initial trigger (first TTL pulse/first plane of the volume)
focal_plane = float(bottom + ((pulse_count - 1) * steps))
#time.sleep(0.064)
print('Focal plane', focal_plane)
etl.current(focal_plane)
from picamera import PiCamera
from opto import Opto
import time
import cv2
import numpy as np
fileDir = "./imgs/"
# Initialize Camera
camera = PiCamera(sensor_mode=7)
camera.color_effects = (128, 128)
camera.resolution = (640, 480)
camera.framerate = 30
# Initialize Tunable Lens
o = Opto(port='/dev/ttyACM0')
o.connect()
o.current(0)
# Allow Camera to warmup
time.sleep(0.5)
# Loop Across all settings
for i in range(-290, 291, 1):
o.current(i)
time.sleep(0.5) # Allow for settling time of the tunable lens
fileName = fileDir + str(i) + ".png"
fileNameAvr = fileDir + str(i) + "_avr.png"
image_avr = np.zeros((480, 640, 3))
for j in range(0, 30):
rawCapture = PiRGBArray(camera, size=(640, 480))
# Script used to tune lens using the CMD
from opto import Opto
import time
o = Opto(port='/dev/ttyACM0')
o.connect()
print('Successfully Connected')
current_low = -285
current_high = 285
current_delta = current_high - current_low
print('Upper: ', current_high)
print('Lower: ', current_low)
print('Input \'q\' to exit')
while True:
key = input("Current:")
try:
key = float(key)
if key > current_low and key < current_high:
o.current(key)
elif key < current_low:
o.current(current_low)
elif key > current_high:
o.current(current_high)
except ValueError:
if key == "q" or key == "Q" or key == "Quit" or key == "quit" or key == "QUIT":
o.current(0)
break
elif key == "scan":
# "a/d" => +/- 10 mA # "-/=" => +/- 1 mA # "0" => 0 mA # "s" => Scan [-290:10:290] # "q" => Quit # import the necessary packages from picamera.array import PiRGBArray from picamera import PiCamera import time import cv2 # New Lines from opto import Opto o = Opto(port='/dev/ttyACM0') o.connect() scan = False current = 0 o.current(current) # initialize the camera and grab a reference to the raw camera capture camera = PiCamera(sensor_mode=7) camera.resolution = (640, 480) camera.framerate = 30 camera.color_effects = (128, 128) rawCapture = PiRGBArray(camera, size=(640, 480)) # cv2 text overlay font = cv2.FONT_HERSHEY_SIMPLEX location1 = (10, 20)
top = 10
bottom = 0
steps = 2
n_planes = 2 * (int(
(top - bottom) / steps) + 1) # total number of planes for each volume
volumes_per_s = fps / n_planes # total number of volume acquisition per volume
pulse_count = 0
delay = 0.075
listdata = []
listtime = []
freq = 15.0
bottom = 0
top = 5
step = 5
with Opto(port='COM6') as o:
o.current(0)
old = 0
while True:
pulse_count = int(np.array(dev.getFeedback(u3.Counter(counter=1))))
if pulse_count > old:
print('pulse count')
current_low = 0
current_high = step
current_delta = current_high - current_low
t0 = time.time()
for i in np.linspace(0, 2 * np.pi, 1000):
listtime.append(time.time() - t0)
listdata.append(
(np.sin(i * 0.25) * current_delta + current_low))
o.current(bottom + np.sin(i * 0.25) * current_delta +
# Script2 to test the functionality of the Opto library from https://github.com/OrganicIrratiation/opto
from opto import Opto
import time
import numpy as np
o = Opto(port='/dev/ttyACM0')
o.connect()
print('Successfully Connected')
current_low = o.current_lower()
current_high = o.current_upper()
current_delta = current_high - current_low
print('Upper: ', current_high)
print('Lower: ', current_low)
o.close(soft_close=True)
help="Optotune Lens setting")
ap.add_argument("-d",
"--display",
type=int,
default=-1,
help="Whether or not frames should be displayed")
args = vars(ap.parse_args())
# Initialize Camera
camera = PiCamera(sensor_mode=7)
camera.color_effects = (128, 128)
camera.resolution = (640, 480)
camera.framerate = 30
# Initialize Tunable Lens
o = Opto(port='/dev/ttyACM0')
o.connect()
o.current(args['lens'])
# Allow Camera to warmup
time.sleep(0.5)
# Set up RGB and YUV Capture
rgbCapture = PiRGBArray(camera, size=(640, 480))
yuvCapture = PiYUVArray(camera, size=(640, 480))
t1 = time.time()
camera.capture(rgbCapture, format="bgr")
t2 = time.time()
camera.capture(yuvCapture, format="yuv")
t3 = time.time()
# Script to test the functionality of the Opto library from https://github.com/OrganicIrratiation/opto
from opto import Opto
import numpy as np
import time
with Opto(port='/dev/ttyACM0') as o:
current_low = o.current_lower()
current_high = o.current_upper()
current_delta = current_high - current_low
for i in np.linspace(0, 2 * np.pi, 1000):
o.current(np.sin(i) * current_delta + current_low)
time.sleep(0.01)