def main(): # Get options args = options() # Read image (converting fmax and track to 8 bit just to create a mask, use 16-bit for all the math) mask, path, filename = pcv.readimage(args.fmax) #mask = cv2.imread(args.fmax) track = cv2.imread(args.track) mask1, mask2, mask3= cv2.split(mask) # Pipeline step device = 0 # Mask pesky track autofluor device, track1= pcv.rgb2gray_hsv(track, 'v', device, args.debug) device, track_thresh = pcv.binary_threshold(track1, 0, 255, 'light', device, args.debug) device, track_inv=pcv.invert(track_thresh, device, args.debug) device, track_masked = pcv.apply_mask(mask1, track_inv, 'black', device, args.debug) # Threshold the Saturation image device, fmax_thresh = pcv.binary_threshold(track_masked, 20, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(fmax_thresh, 0, device, args.debug) device, s_cnt = pcv.median_blur(fmax_thresh, 0, device, args.debug) # Fill small objects device, s_fill = pcv.fill(s_mblur, s_cnt, 5, device, args.debug) device, sfill_cnt = pcv.fill(s_mblur, s_cnt, 5, device, args.debug) # Identify objects device, id_objects,obj_hierarchy = pcv.find_objects(mask, sfill_cnt, device, args.debug) # Define ROI device, roi1, roi_hierarchy= pcv.define_roi(mask,'circle', device, None, 'default', args.debug,True, 0,0,-100,-100) # Decide which objects to keep device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(mask,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug) # Object combine kept objects device, obj, masked = pcv.object_composition(mask, roi_objects, hierarchy3, device, args.debug) ################ Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(mask, args.fmax, obj, masked, device,args.debug, args.outdir+'/'+filename) # Fluorescence Measurement (read in 16-bit images) fdark=cv2.imread(args.fdark, -1) fmin=cv2.imread(args.fmin, -1) fmax=cv2.imread(args.fmax, -1) device, fvfm_header, fvfm_data=pcv.fluor_fvfm(fdark,fmin,fmax,kept_mask, device, args.outdir+'/'+filename, 1000, args.debug) # Output shape and color data pcv.print_results(args.fmax, shape_header, shape_data) pcv.print_results(args.fmax, fvfm_header, fvfm_data)
def main(): # Get options args = options() # Read image img = cv2.imread(args.image) roi = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 36, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug) device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug) # Fill small objects device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug) # Convert RGB to LAB and extract the Blue channel device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug) # Threshold the blue image device, b_thresh = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) device, b_cnt = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) # Fill small objects device, b_fill = pcv.fill(b_thresh, b_cnt, 150, device, args.debug) # Join the thresholded saturation and blue-yellow images device, bs = pcv.logical_and(s_fill, b_fill, device, args.debug) # Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) device, masked_b = pcv.rgb2gray_lab(masked, 'b', device, args.debug) # Threshold the green-magenta and blue images device, maskeda_thresh = pcv.binary_threshold(masked_a, 122, 255, 'dark', device, args.debug) device, maskedb_thresh = pcv.binary_threshold(masked_b, 133, 255, 'light', device, args.debug) # Join the thresholded saturation and blue-yellow images (OR) device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) # Fill small objects device, ab_fill = pcv.fill(ab, ab_cnt, 200, device, args.debug) # Apply mask (for vis images, mask_color=white) device, masked2 = pcv.apply_mask(masked, ab_fill, 'white', device, args.debug) # Identify objects device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug) # Define ROI device, roi1, roi_hierarchy= pcv.define_roi(img,'rectangle', device, None, 'default', args.debug,False, 0, 0,0,0) # Decide which objects to keep device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug) # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug) ############## Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,True) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,'all','rgb','v') # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data)
def main():
# Get options
args = options()
if args.debug:
print("Analyzing your image dude...")
# Read image
device = 0
img = cv2.imread(args.image, flags=0)
path, img_name = os.path.split(args.image)
# Read in image which is average of average of backgrounds
img_bkgrd = cv2.imread("bkgrd_ave_z2500.png", flags=0)
# NIR images for burnin2 are up-side down. This may be fixed in later experiments
img = ndimage.rotate(img, 180)
img_bkgrd = ndimage.rotate(img_bkgrd, 180)
# Subtract the image from the image background to make the plant more prominent
device, bkg_sub_img = pcv.image_subtract(img, img_bkgrd, device, args.debug)
if args.debug:
pcv.plot_hist(bkg_sub_img, "bkg_sub_img")
device, bkg_sub_thres_img = pcv.binary_threshold(bkg_sub_img, 145, 255, "dark", device, args.debug)
bkg_sub_thres_img = cv2.inRange(bkg_sub_img, 30, 220)
if args.debug:
cv2.imwrite("bkgrd_sub_thres.png", bkg_sub_thres_img)
# device, bkg_sub_thres_img = pcv.binary_threshold_2_sided(img_bkgrd, 50, 190, device, args.debug)
# if a region of interest is specified read it in
roi = cv2.imread(args.roi)
# Start by examining the distribution of pixel intensity values
if args.debug:
pcv.plot_hist(img, "hist_img")
# Will intensity transformation enhance your ability to isolate object of interest by thesholding?
device, he_img = pcv.HistEqualization(img, device, args.debug)
if args.debug:
pcv.plot_hist(he_img, "hist_img_he")
# Laplace filtering (identify edges based on 2nd derivative)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(lp_img, "hist_lp")
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
if args.debug:
pcv.plot_hist(lp_shrp_img, "hist_lp_shrp")
# Sobel filtering
# 1st derivative sobel filtering along horizontal axis, kernel = 1, unscaled)
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sbx_img, "hist_sbx")
# 1st derivative sobel filtering along vertical axis, kernel = 1, unscaled)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sby_img, "hist_sby")
# Combine the effects of both x and y filters through matrix addition
# This will capture edges identified within each plane and emphesize edges found in both images
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
if args.debug:
pcv.plot_hist(sb_img, "hist_sb_comb_img")
# Use a lowpass (blurring) filter to smooth sobel image
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
# combine the smoothed sobel image with the laplacian sharpened image
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img, device, args.debug)
if args.debug:
pcv.plot_hist(edge_shrp_img, "hist_edge_shrp_img")
# Perform thresholding to generate a binary image
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 145, 255, "dark", device, args.debug)
# Prepare a few small kernels for morphological filtering
kern = np.zeros((3, 3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1, 1:3] = 1
kern2 = np.copy(kern)
kern2[1, 0:2] = 1
kern3 = np.copy(kern)
kern3[0:2, 1] = 1
kern4 = np.copy(kern)
kern4[1:3, 1] = 1
# Prepare a larger kernel for dilation
kern[1, 0:3] = 1
kern[0:3, 1] = 1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, kern1, 1, device, args.debug)
device, e2_img = pcv.erode(tr_es_img, kern2, 1, device, args.debug)
device, e3_img = pcv.erode(tr_es_img, kern3, 1, device, args.debug)
device, e4_img = pcv.erode(tr_es_img, kern4, 1, device, args.debug)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
# Perform dilation
# device, dil_img = pcv.dilate(c1234_img, kern, 1, device, args.debug)
device, comb_img = pcv.logical_or(c1234_img, bkg_sub_thres_img, device, args.debug)
# Get masked image
# The dilated image may contain some pixels which are not plant
device, masked_erd = pcv.apply_mask(img, comb_img, "black", device, args.debug)
# device, masked_erd_dil = pcv.apply_mask(img, dil_img, 'black', device, args.debug)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
# mask for the bottom of the image
device, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(img, (120, 184), (215, 252), device, args.debug)
# mask for the left side of the image
device, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(img, (1, 1), (85, 252), device, args.debug)
# mask for the right side of the image
device, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(img, (240, 1), (318, 252), device, args.debug)
# mask the edges
device, box4_img, rect_contour4, hierarchy4 = pcv.border_mask(img, (1, 1), (318, 252), device, args.debug)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device, args.debug)
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# Make a ROI around the plant, include connected objects
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, edge_masked_img = pcv.apply_mask(masked_erd, inv_bx1234_img, "black", device, args.debug)
device, roi_img, roi_contour, roi_hierarchy = pcv.rectangle_mask(img, (120, 75), (200, 184), device, args.debug)
plant_objects, plant_hierarchy = cv2.findContours(edge_masked_img, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)
device, roi_objects, hierarchy5, kept_mask, obj_area = pcv.roi_objects(
img, "partial", roi_contour, roi_hierarchy, plant_objects, plant_hierarchy, device, args.debug
)
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, "black", device, args.debug)
rgb = cv2.cvtColor(img, cv2.COLOR_GRAY2RGB)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, "light", device, args.debug)
mask3d = np.copy(mask)
plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)
device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
### Analysis ###
device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(
img, args.image, mask, 256, device, args.debug, args.outdir + "/" + img_name
)
device, shape_header, shape_data, ori_img = pcv.analyze_object(
rgb, args.image, o, m, device, args.debug, args.outdir + "/" + img_name
)
pcv.print_results(args.image, hist_header, hist_data)
pcv.print_results(args.image, shape_header, shape_data)
def main():
# Get options
args = options()
# Read image
img, path, filename = pcv.readimage(args.image)
# roi = cv2.imread(args.roi)
# Pipeline step
device = 0
## Convert RGB to HSV and extract the Saturation channel
# device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug)
#
## Threshold the Saturation image
# device, s_thresh = pcv.binary_threshold(s, 90, 255, 'dark', device, args.debug)
#
## Median Filter
# device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug)
# device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug)
#
## Fill small objects
##device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug)
#
## Convert RGB to LAB and extract the Blue channel
# device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug)
#
## Threshold the blue image
# device, b_thresh = pcv.binary_threshold(b, 135, 255, 'light', device, args.debug)
# device, b_cnt = pcv.binary_threshold(b, 135, 255, 'light', device, args.debug)
#
##Fill small objects
# device, b_fill = pcv.fill(b_thresh, b_cnt, 10, device, args.debug)
#
## Join the thresholded saturation and blue-yellow images
# device, bs = pcv.logical_or(s_mblur, b_cnt, device, args.debug)
#
## Apply Mask (for vis images, mask_color=white)
# device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug)
# Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels
device, masked_a = pcv.rgb2gray_lab(img, "a", device, args.debug)
device, masked_b = pcv.rgb2gray_lab(img, "b", device, args.debug)
# Threshold the green-magenta and blue images
device, maskeda_thresh = pcv.binary_threshold(masked_a, 135, 255, "dark", device, args.debug)
device, maskedb_thresh = pcv.binary_threshold(masked_b, 140, 255, "light", device, args.debug)
#
# # Join the thresholded saturation and blue-yellow images (OR)
device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug)
device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug)
# Fill small objects
device, ab_fill = pcv.fill(ab, ab_cnt, 1000, device, args.debug)
# Apply mask (for vis images, mask_color=white)
device, masked2 = pcv.apply_mask(img, ab_fill, "white", device, args.debug)
# Identify objects
device, id_objects, obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug)
# Define ROI
device, roi1, roi_hierarchy = pcv.define_roi(
masked2, "rectangle", device, None, "default", args.debug, True, 550, 0, -500, -300
)
# Decide which objects to keep
device, roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(
img, "partial", roi1, roi_hierarchy, id_objects, obj_hierarchy, device, args.debug
)
# Object combine kept objects
device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
############### Analysis ################
# Find shape properties, output shape image (optional)
device, shape_header, shape_data, shape_img = pcv.analyze_object(
img, args.image, obj, mask, device, args.debug, args.outdir + "/" + filename
)
# Shape properties relative to user boundary line (optional)
# device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 1680, device,args.debug,args.outdir+'/'+filename)
# Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
device, color_header, color_data, norm_slice = pcv.analyze_color(
img, args.image, kept_mask, 256, device, args.debug, "all", "rgb", "v", "img", 300, args.outdir + "/" + filename
)
# Output shape and color data
pcv.print_results(args.image, shape_header, shape_data)
pcv.print_results(args.image, color_header, color_data)
def main(): # Get options args = options() # Read image img, path, filename = pcv.readimage(args.image) #roi = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 36, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug) device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug) # Fill small objects device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug) # Convert RGB to LAB and extract the Blue channel device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug) # Threshold the blue image device, b_thresh = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) device, b_cnt = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) # Fill small objects device, b_fill = pcv.fill(b_thresh, b_cnt, 150, device, args.debug) # Join the thresholded saturation and blue-yellow images device, bs = pcv.logical_and(s_fill, b_fill, device, args.debug) # Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) device, masked_b = pcv.rgb2gray_lab(masked, 'b', device, args.debug) # Threshold the green-magenta and blue images device, maskeda_thresh = pcv.binary_threshold(masked_a, 122, 255, 'dark', device, args.debug) device, maskedb_thresh = pcv.binary_threshold(masked_b, 133, 255, 'light', device, args.debug) # Join the thresholded saturation and blue-yellow images (OR) device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) # Fill small objects device, ab_fill = pcv.fill(ab, ab_cnt, 200, device, args.debug) # Apply mask (for vis images, mask_color=white) device, masked2 = pcv.apply_mask(masked, ab_fill, 'white', device, args.debug) # Select area with black bars and find overlapping plant material device, roi1, roi_hierarchy1= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 0, 0,-1900,0) device, id_objects1,obj_hierarchy1 = pcv.find_objects(masked2, ab_fill, device, args.debug) device,roi_objects1, hierarchy1, kept_mask1, obj_area1 = pcv.roi_objects(masked2,'cutto',roi1,roi_hierarchy1,id_objects1,obj_hierarchy1,device, args.debug) device, masked3 = pcv.apply_mask(masked2, kept_mask1, 'white', device, args.debug) device, masked_a1 = pcv.rgb2gray_lab(masked3, 'a', device, args.debug) device, masked_b1 = pcv.rgb2gray_lab(masked3, 'b', device, args.debug) device, maskeda_thresh1 = pcv.binary_threshold(masked_a1, 122, 255, 'dark', device, args.debug) device, maskedb_thresh1 = pcv.binary_threshold(masked_b1, 170, 255, 'light', device, args.debug) device, ab1 = pcv.logical_or(maskeda_thresh1, maskedb_thresh1, device, args.debug) device, ab_cnt1 = pcv.logical_or(maskeda_thresh1, maskedb_thresh1, device, args.debug) device, ab_fill1 = pcv.fill(ab1, ab_cnt1, 300, device, args.debug) device, roi2, roi_hierarchy2= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 1900, 0,0,0) device, id_objects2,obj_hierarchy2 = pcv.find_objects(masked2, ab_fill, device, args.debug) device,roi_objects2, hierarchy2, kept_mask2, obj_area2 = pcv.roi_objects(masked2,'cutto',roi2,roi_hierarchy2,id_objects2,obj_hierarchy2,device, args.debug) device, masked4 = pcv.apply_mask(masked2, kept_mask2, 'white', device, args.debug) device, masked_a2 = pcv.rgb2gray_lab(masked4, 'a', device, args.debug) device, masked_b2 = pcv.rgb2gray_lab(masked4, 'b', device, args.debug) device, maskeda_thresh2 = pcv.binary_threshold(masked_a2, 122, 255, 'dark', device, args.debug) device, maskedb_thresh2 = pcv.binary_threshold(masked_b2, 170, 255, 'light', device, args.debug) device, ab2 = pcv.logical_or(maskeda_thresh2, maskedb_thresh2, device, args.debug) device, ab_cnt2 = pcv.logical_or(maskeda_thresh2, maskedb_thresh2, device, args.debug) device, ab_fill2 = pcv.fill(ab2, ab_cnt2, 200, device, args.debug) device, ab_cnt3 = pcv.logical_or(ab_fill1, ab_fill2, device, args.debug) device, masked3 = pcv.apply_mask(masked2, ab_cnt3, 'white', device, args.debug) # Identify objects device, id_objects3,obj_hierarchy3 = pcv.find_objects(masked2, ab_fill, device, args.debug) # Define ROI device, roi3, roi_hierarchy3= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 500, 0,-450,-530) # Decide which objects to keep and combine with objects overlapping with black bars device,roi_objects3, hierarchy3, kept_mask3, obj_area1 = pcv.roi_objects(img,'cutto',roi3,roi_hierarchy3,id_objects3,obj_hierarchy3,device, args.debug) device, kept_mask4_1 = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug) device, kept_cnt = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug) device, kept_mask4 = pcv.fill(kept_mask4_1, kept_cnt, 200, device, args.debug) device, masked5 = pcv.apply_mask(masked2, kept_mask4, 'white', device, args.debug) device, id_objects4,obj_hierarchy4 = pcv.find_objects(masked5, kept_mask4, device, args.debug) device, roi4, roi_hierarchy4= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,False, 0, 0,0,0) device,roi_objects4, hierarchy4, kept_mask4, obj_area = pcv.roi_objects(img,'partial',roi4,roi_hierarchy4,id_objects4,obj_hierarchy4,device, args.debug) # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects4, hierarchy4, device, args.debug) ############## Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename) # Shape properties relative to user boundary line (optional) device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 950, device,args.debug,args.outdir+'/'+filename) # Tiller Tool Test device, tillering_header, tillering_data, tillering_img= pcv.tiller_count(img, args.image,obj, mask, 965, device,args.debug,args.outdir+'/'+filename) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask4, 256, device, args.debug,'all','rgb','v',args.outdir+'/'+filename) # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data) pcv.print_results(args.image, boundary_header, boundary_data) pcv.print_results(args.image, tillering_header,tillering_data)
def main():
# Get options
args = options()
# Read image
img, path, filename = pcv.readimage(args.image)
brass_mask = cv2.imread(args.roi)
# Pipeline step
device = 0
# Convert RGB to HSV and extract the Saturation channel
device, s = pcv.rgb2gray_hsv(img, "s", device, args.debug)
# Threshold the Saturation image
device, s_thresh = pcv.binary_threshold(s, 49, 255, "light", device, args.debug)
# Median Filter
device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug)
device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug)
# Fill small objects
device, s_fill = pcv.fill(s_mblur, s_cnt, 150, device, args.debug)
# Convert RGB to LAB and extract the Blue channel
device, b = pcv.rgb2gray_lab(img, "b", device, args.debug)
# Threshold the blue image
device, b_thresh = pcv.binary_threshold(b, 138, 255, "light", device, args.debug)
device, b_cnt = pcv.binary_threshold(b, 138, 255, "light", device, args.debug)
# Fill small objects
device, b_fill = pcv.fill(b_thresh, b_cnt, 150, device, args.debug)
# Join the thresholded saturation and blue-yellow images
device, bs = pcv.logical_and(s_fill, b_fill, device, args.debug)
# Apply Mask (for vis images, mask_color=white)
device, masked = pcv.apply_mask(img, bs, "white", device, args.debug)
# Mask pesky brass piece
device, brass_mask1 = pcv.rgb2gray_hsv(brass_mask, "v", device, args.debug)
device, brass_thresh = pcv.binary_threshold(brass_mask1, 0, 255, "light", device, args.debug)
device, brass_inv = pcv.invert(brass_thresh, device, args.debug)
device, brass_masked = pcv.apply_mask(masked, brass_inv, "white", device, args.debug)
# Further mask soil and car
device, masked_a = pcv.rgb2gray_lab(brass_masked, "a", device, args.debug)
device, soil_car = pcv.binary_threshold(masked_a, 128, 255, "dark", device, args.debug)
device, soil_masked = pcv.apply_mask(brass_masked, soil_car, "white", device, args.debug)
# Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels
device, soil_a = pcv.rgb2gray_lab(soil_masked, "a", device, args.debug)
device, soil_b = pcv.rgb2gray_lab(soil_masked, "b", device, args.debug)
# Threshold the green-magenta and blue images
device, soila_thresh = pcv.binary_threshold(soil_a, 118, 255, "dark", device, args.debug)
device, soilb_thresh = pcv.binary_threshold(soil_b, 150, 255, "light", device, args.debug)
# Join the thresholded saturation and blue-yellow images (OR)
device, soil_ab = pcv.logical_or(soila_thresh, soilb_thresh, device, args.debug)
device, soil_ab_cnt = pcv.logical_or(soila_thresh, soilb_thresh, device, args.debug)
# Fill small objects
device, soil_cnt = pcv.fill(soil_ab, soil_ab_cnt, 75, device, args.debug)
# Median Filter
# device, soil_mblur = pcv.median_blur(soil_fill, 5, device, args.debug)
# device, soil_cnt = pcv.median_blur(soil_fill, 5, device, args.debug)
# Apply mask (for vis images, mask_color=white)
device, masked2 = pcv.apply_mask(soil_masked, soil_cnt, "white", device, args.debug)
# Identify objects
device, id_objects, obj_hierarchy = pcv.find_objects(masked2, soil_cnt, device, args.debug)
# Define ROI
device, roi1, roi_hierarchy = pcv.define_roi(
img, "circle", device, None, "default", args.debug, True, 0, 0, -200, -200
)
# Decide which objects to keep
device, roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(
img, "partial", roi1, roi_hierarchy, id_objects, obj_hierarchy, device, args.debug
)
# Object combine kept objects
device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
############## Analysis ################
# Find shape properties, output shape image (optional)
device, shape_header, shape_data, shape_img = pcv.analyze_object(
img, args.image, obj, mask, device, args.debug, args.outdir + "/" + filename
)
# Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
device, color_header, color_data, norm_slice = pcv.analyze_color(
img, args.image, kept_mask, 256, device, args.debug, "all", "rgb", "v", "img", 300, args.outdir + "/" + filename
)
# Output shape and color data
pcv.print_results(args.image, shape_header, shape_data)
pcv.print_results(args.image, color_header, color_data)
def main(): # Get options args = options() # Read image img, path, filename = pcv.readimage(args.image) brass_mask = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 49, 255, 'light', device, args.debug) #Median Filter device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug) #Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, s_mblur, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta device, soil_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) # # Threshold the green-magenta device, soila_thresh = pcv.binary_threshold(soil_a, 133, 255, 'light', device, args.debug) device, soila_cnt = pcv.binary_threshold(soil_a, 133, 255, 'light', device, args.debug) # # Fill small objects device, soil_fill = pcv.fill(soila_thresh, soila_cnt, 200, device, args.debug) # # Median Filter device, soil_mblur = pcv.median_blur(soil_fill, 13, device, args.debug) device, soil_cnt = pcv.median_blur(soil_fill, 13, device, args.debug) # # Apply mask (for vis images, mask_color=white) device, masked2 = pcv.apply_mask(soil_mblur, soil_cnt, 'white', device, args.debug) # # Identify objects device, id_objects,obj_hierarchy = pcv.find_objects(masked2, soil_cnt, device, args.debug) # # Define ROI device, roi1, roi_hierarchy= pcv.define_roi(img,'rectangle', device, None, 'default', args.debug,True, 400,400,-400,-400) # # Decide which objects to keep device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug) # # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug) # ############## Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, mask, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename) # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data)
def main(): # Get options args = options() # Read image img, path, filename = pcv.readimage(args.image) #roi = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 36, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(s_thresh, 0, device, args.debug) device, s_cnt = pcv.median_blur(s_thresh, 0, device, args.debug) # Fill small objects #device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug) # Convert RGB to LAB and extract the Blue channel device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug) # Threshold the blue image device, b_thresh = pcv.binary_threshold(b, 137, 255, 'light', device, args.debug) device, b_cnt = pcv.binary_threshold(b, 137, 255, 'light', device, args.debug) # Fill small objects #device, b_fill = pcv.fill(b_thresh, b_cnt, 150, device, args.debug) # Join the thresholded saturation and blue-yellow images device, bs = pcv.logical_and(s_mblur, b_cnt, device, args.debug) # Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) device, masked_b = pcv.rgb2gray_lab(masked, 'b', device, args.debug) # Threshold the green-magenta and blue images device, maskeda_thresh = pcv.binary_threshold(masked_a, 127, 255, 'dark', device, args.debug) device, maskedb_thresh = pcv.binary_threshold(masked_b, 128, 255, 'light', device, args.debug) # Join the thresholded saturation and blue-yellow images (OR) device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) # Fill small noise device, ab_fill1 = pcv.fill(ab, ab_cnt, 2, device, args.debug) # Dilate to join small objects with larger ones device, ab_cnt1=pcv.dilate(ab_fill1, 3, 2, device, args.debug) device, ab_cnt2=pcv.dilate(ab_fill1, 3, 2, device, args.debug) # Fill dilated image mask device, ab_cnt3=pcv.fill(ab_cnt2,ab_cnt1,150,device,args.debug) device, masked2 = pcv.apply_mask(masked, ab_cnt3, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked2_a = pcv.rgb2gray_lab(masked2, 'a', device, args.debug) device, masked2_b = pcv.rgb2gray_lab(masked2, 'b', device, args.debug) # Threshold the green-magenta and blue images device, masked2a_thresh = pcv.binary_threshold(masked2_a, 127, 255, 'dark', device, args.debug) device, masked2b_thresh = pcv.binary_threshold(masked2_b, 128, 255, 'light', device, args.debug) device, ab_fill = pcv.logical_or(masked2a_thresh, masked2b_thresh, device, args.debug) # Identify objects device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug) # Define ROI device, roi1, roi_hierarchy= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 525, 0,-490,-150) # Decide which objects to keep device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug) # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug) ############## Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename) # Shape properties relative to user boundary line (optional) device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 325, device,args.debug,args.outdir+'/'+filename) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,color_img= pcv.analyze_color(img, args.image, mask, 256, device, args.debug,None,'v','img',300,args.outdir+'/'+filename) # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data) pcv.print_results(args.image, boundary_header, boundary_data)
def test_plantcv_print_results():
header = ['field1', 'field2', 'field3']
data = ['value1', 'value2', 'value3']
pcv.print_results(filename='not_used', header=header, data=data)
def main(): # Get options args = options() # Read image img, path, filename = pcv.readimage(args.image) #roi = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 36, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug) device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug) # Fill small objects device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug) # Convert RGB to LAB and extract the Blue channel device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug) # Threshold the blue image device, b_thresh = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) device, b_cnt = pcv.binary_threshold(b, 138, 255, 'light', device, args.debug) # Fill small objects device, bs = pcv.fill(b_thresh, b_cnt, 100, device, args.debug) # Join the thresholded saturation and blue-yellow images #device, bs = pcv.logical_and(s_fill, b_fill, device, args.debug) # Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) device, masked_b = pcv.rgb2gray_lab(masked, 'b', device, args.debug) # Threshold the green-magenta and blue images device, maskeda_thresh = pcv.binary_threshold(masked_a, 122, 255, 'dark', device, args.debug) device, maskedb_thresh = pcv.binary_threshold(masked_b, 133, 255, 'light', device, args.debug) # Join the thresholded saturation and blue-yellow images (OR) device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) # Fill small objects device, ab_fill = pcv.fill(ab, ab_cnt, 100, device, args.debug) # Apply mask (for vis images, mask_color=white) device, masked2 = pcv.apply_mask(masked, ab_fill, 'white', device, args.debug) # Select area with black bars and find overlapping plant material device, roi1, roi_hierarchy1= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 0, 0,-1700,0) device, id_objects1,obj_hierarchy1 = pcv.find_objects(masked2, ab_fill, device, args.debug) device,roi_objects1, hierarchy1, kept_mask1, obj_area1 = pcv.roi_objects(masked2,'cutto',roi1,roi_hierarchy1,id_objects1,obj_hierarchy1,device, args.debug) device, masked3 = pcv.apply_mask(masked2, kept_mask1, 'white', device, args.debug) device, masked_a1 = pcv.rgb2gray_lab(masked3, 'a', device, args.debug) device, masked_b1 = pcv.rgb2gray_lab(masked3, 'b', device, args.debug) device, maskeda_thresh1 = pcv.binary_threshold(masked_a1, 122, 255, 'dark', device, args.debug) device, maskedb_thresh1 = pcv.binary_threshold(masked_b1, 170, 255, 'light', device, args.debug) device, ab1 = pcv.logical_or(maskeda_thresh1, maskedb_thresh1, device, args.debug) device, ab_cnt1 = pcv.logical_or(maskeda_thresh1, maskedb_thresh1, device, args.debug) device, ab_fill1 = pcv.fill(ab1, ab_cnt1, 300, device, args.debug) device, roi2, roi_hierarchy2= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 1700, 0,0,0) device, id_objects2,obj_hierarchy2 = pcv.find_objects(masked2, ab_fill, device, args.debug) device,roi_objects2, hierarchy2, kept_mask2, obj_area2 = pcv.roi_objects(masked2,'cutto',roi2,roi_hierarchy2,id_objects2,obj_hierarchy2,device, args.debug) device, masked4 = pcv.apply_mask(masked2, kept_mask2, 'white', device, args.debug) device, masked_a2 = pcv.rgb2gray_lab(masked4, 'a', device, args.debug) device, masked_b2 = pcv.rgb2gray_lab(masked4, 'b', device, args.debug) device, maskeda_thresh2 = pcv.binary_threshold(masked_a2, 122, 255, 'dark', device, args.debug) device, maskedb_thresh2 = pcv.binary_threshold(masked_b2, 170, 255, 'light', device, args.debug) device, ab2 = pcv.logical_or(maskeda_thresh2, maskedb_thresh2, device, args.debug) device, ab_cnt2 = pcv.logical_or(maskeda_thresh2, maskedb_thresh2, device, args.debug) device, ab_fill2 = pcv.fill(ab2, ab_cnt2, 450, device, args.debug) device, ab_cnt3 = pcv.logical_or(ab_fill1, ab_fill2, device, args.debug) device, masked3 = pcv.apply_mask(masked2, ab_cnt3, 'white', device, args.debug) # Identify objects device, id_objects3,obj_hierarchy3 = pcv.find_objects(masked2, ab_fill, device, args.debug) # Define ROI device, roi3, roi_hierarchy3= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 650, 0,-650,-300) # Decide which objects to keep and combine with objects overlapping with black bars device,roi_objects3, hierarchy3, kept_mask3, obj_area1 = pcv.roi_objects(img,'cutto',roi3,roi_hierarchy3,id_objects3,obj_hierarchy3,device, args.debug) device, kept_mask4_1 = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug) device, kept_cnt = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug) device, kept_mask4 = pcv.fill(kept_mask4_1, kept_cnt, 200, device, args.debug) device, masked5 = pcv.apply_mask(masked2, kept_mask4, 'white', device, args.debug) device, id_objects4,obj_hierarchy4 = pcv.find_objects(masked5, kept_mask4, device, args.debug) device, roi4, roi_hierarchy4= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,False, 0, 0,0,0) device,roi_objects4, hierarchy4, kept_mask4, obj_area = pcv.roi_objects(img,'partial',roi4,roi_hierarchy4,id_objects4,obj_hierarchy4,device, args.debug) # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects4, hierarchy4, device, args.debug) ############### Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename) # Shape properties relative to user boundary line (optional) device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 380, device,args.debug,args.outdir+'/'+filename) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask4, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename) # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data) pcv.print_results(args.image, boundary_header, boundary_data)
def main():
# Get options
args = options()
if args.debug:
print("Analyzing your image dude...")
# Read image
device = 0
img = cv2.imread(args.image, flags=0)
path, img_name = os.path.split(args.image)
# Read in image which is average of average of backgrounds
img_bkgrd = cv2.imread("bkgrd_ave_z500.png", flags=0)
# NIR images for burnin2 are up-side down. This may be fixed in later experiments
img = ndimage.rotate(img, 180)
img_bkgrd = ndimage.rotate(img_bkgrd, 180)
# Subtract the image from the image background to make the plant more prominent
device, bkg_sub_img = pcv.image_subtract(img, img_bkgrd, device, args.debug)
if args.debug:
pcv.plot_hist(bkg_sub_img, 'bkg_sub_img')
device, bkg_sub_thres_img = pcv.binary_threshold(bkg_sub_img, 145, 255, 'dark', device, args.debug)
bkg_sub_thres_img = cv2.inRange(bkg_sub_img, 50, 190)
if args.debug:
cv2.imwrite('bkgrd_sub_thres.png', bkg_sub_thres_img)
#device, bkg_sub_thres_img = pcv.binary_threshold_2_sided(img_bkgrd, 50, 190, device, args.debug)
# if a region of interest is specified read it in
roi = cv2.imread(args.roi)
# Start by examining the distribution of pixel intensity values
if args.debug:
pcv.plot_hist(img, 'hist_img')
# Will intensity transformation enhance your ability to isolate object of interest by thesholding?
device, he_img = pcv.HistEqualization(img, device, args.debug)
if args.debug:
pcv.plot_hist(he_img, 'hist_img_he')
# Laplace filtering (identify edges based on 2nd derivative)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(lp_img, 'hist_lp')
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
if args.debug:
pcv.plot_hist(lp_shrp_img, 'hist_lp_shrp')
# Sobel filtering
# 1st derivative sobel filtering along horizontal axis, kernel = 1, unscaled)
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sbx_img, 'hist_sbx')
# 1st derivative sobel filtering along vertical axis, kernel = 1, unscaled)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sby_img, 'hist_sby')
# Combine the effects of both x and y filters through matrix addition
# This will capture edges identified within each plane and emphesize edges found in both images
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
if args.debug:
pcv.plot_hist(sb_img, 'hist_sb_comb_img')
# Use a lowpass (blurring) filter to smooth sobel image
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
# combine the smoothed sobel image with the laplacian sharpened image
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img, device, args.debug)
if args.debug:
pcv.plot_hist(edge_shrp_img, 'hist_edge_shrp_img')
# Perform thresholding to generate a binary image
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 145, 255, 'dark', device, args.debug)
# Prepare a few small kernels for morphological filtering
kern = np.zeros((3,3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1,1:3]=1
kern2 = np.copy(kern)
kern2[1,0:2]=1
kern3 = np.copy(kern)
kern3[0:2,1]=1
kern4 = np.copy(kern)
kern4[1:3,1]=1
# Prepare a larger kernel for dilation
kern[1,0:3]=1
kern[0:3,1]=1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, kern1, 1, device, args.debug)
device, e2_img = pcv.erode(tr_es_img, kern2, 1, device, args.debug)
device, e3_img = pcv.erode(tr_es_img, kern3, 1, device, args.debug)
device, e4_img = pcv.erode(tr_es_img, kern4, 1, device, args.debug)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
# Perform dilation
# device, dil_img = pcv.dilate(c1234_img, kern, 1, device, args.debug)
device, comb_img = pcv.logical_or(c1234_img, bkg_sub_thres_img, device, args.debug)
# Get masked image
# The dilated image may contain some pixels which are not plant
device, masked_erd = pcv.apply_mask(img, comb_img, 'black', device, args.debug)
# device, masked_erd_dil = pcv.apply_mask(img, dil_img, 'black', device, args.debug)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
# mask for the bottom of the image
device, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(img, (128,226), (192,252), device, args.debug)
# mask for the left side of the image
device, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(img, (1,1), (75,252), device, args.debug)
# mask for the right side of the image
device, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(img, (245,1), (318,252), device, args.debug)
# mask the edges
device, box4_img, rect_contour4, hierarchy4 = pcv.border_mask(img, (1,1), (318,252), device, args.debug)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device, args.debug)
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# Make a ROI around the plant, include connected objects
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, edge_masked_img = pcv.apply_mask(masked_erd, inv_bx1234_img, 'black', device, args.debug)
device, roi_img, roi_contour, roi_hierarchy = pcv.rectangle_mask(img, (120,75), (200,226), device, args.debug)
plant_objects, plant_hierarchy = cv2.findContours(edge_masked_img,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
device, roi_objects, hierarchy5, kept_mask, obj_area = pcv.roi_objects(img, 'partial', roi_contour, roi_hierarchy, plant_objects, plant_hierarchy, device, args.debug)
# Apply the box mask to the image
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, 'black', device, args.debug)
rgb = cv2.cvtColor(img,cv2.COLOR_GRAY2RGB)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, 'light', device, args.debug)
mask3d = np.copy(mask)
plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
### Analysis ###
device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(img, args.image, mask, 256, device, args.debug, args.outdir + '/' + img_name)
device, shape_header, shape_data, ori_img = pcv.analyze_object(rgb, args.image, o, m, device, args.debug, args.outdir + '/' + img_name)
pcv.print_results(args.image, hist_header, hist_data)
pcv.print_results(args.image, shape_header, shape_data)
def main():
# Get options
args = options()
# Read image
img, path, filename = pcv.readimage(args.image)
brass_mask = cv2.imread(args.roi)
# Pipeline step
device = 0
# Convert RGB to HSV and extract the Saturation channel
device, s = pcv.rgb2gray_hsv(img, 's', device, args.debug)
# Threshold the Saturation image
device, s_thresh = pcv.binary_threshold(s, 49, 255, 'light', device,
args.debug)
# Median Filter
device, s_mblur = pcv.median_blur(s_thresh, 5, device, args.debug)
device, s_cnt = pcv.median_blur(s_thresh, 5, device, args.debug)
# Fill small objects
device, s_fill = pcv.fill(s_mblur, s_cnt, 150, device, args.debug)
# Convert RGB to LAB and extract the Blue channel
device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug)
# Threshold the blue image
device, b_thresh = pcv.binary_threshold(b, 138, 255, 'light', device,
args.debug)
device, b_cnt = pcv.binary_threshold(b, 138, 255, 'light', device,
args.debug)
# Fill small objects
device, b_fill = pcv.fill(b_thresh, b_cnt, 150, device, args.debug)
# Join the thresholded saturation and blue-yellow images
device, bs = pcv.logical_and(s_fill, b_fill, device, args.debug)
# Apply Mask (for vis images, mask_color=white)
device, masked = pcv.apply_mask(img, bs, 'white', device, args.debug)
# Mask pesky brass piece
device, brass_mask1 = pcv.rgb2gray_hsv(brass_mask, 'v', device, args.debug)
device, brass_thresh = pcv.binary_threshold(brass_mask1, 0, 255, 'light',
device, args.debug)
device, brass_inv = pcv.invert(brass_thresh, device, args.debug)
device, brass_masked = pcv.apply_mask(masked, brass_inv, 'white', device,
args.debug)
# Further mask soil and car
device, masked_a = pcv.rgb2gray_lab(brass_masked, 'a', device, args.debug)
device, soil_car = pcv.binary_threshold(masked_a, 128, 255, 'dark', device,
args.debug)
device, soil_masked = pcv.apply_mask(brass_masked, soil_car, 'white',
device, args.debug)
# Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels
device, soil_a = pcv.rgb2gray_lab(soil_masked, 'a', device, args.debug)
device, soil_b = pcv.rgb2gray_lab(soil_masked, 'b', device, args.debug)
# Threshold the green-magenta and blue images
device, soila_thresh = pcv.binary_threshold(soil_a, 118, 255, 'dark',
device, args.debug)
device, soilb_thresh = pcv.binary_threshold(soil_b, 155, 255, 'light',
device, args.debug)
# Join the thresholded saturation and blue-yellow images (OR)
device, soil_ab = pcv.logical_or(soila_thresh, soilb_thresh, device,
args.debug)
device, soil_ab_cnt = pcv.logical_or(soila_thresh, soilb_thresh, device,
args.debug)
# Fill small objects
device, soil_fill = pcv.fill(soil_ab, soil_ab_cnt, 200, device, args.debug)
# Median Filter
device, soil_mblur = pcv.median_blur(soil_fill, 5, device, args.debug)
device, soil_cnt = pcv.median_blur(soil_fill, 5, device, args.debug)
# Apply mask (for vis images, mask_color=white)
device, masked2 = pcv.apply_mask(soil_masked, soil_cnt, 'white', device,
args.debug)
# Identify objects
device, id_objects, obj_hierarchy = pcv.find_objects(
masked2, soil_cnt, device, args.debug)
# Define ROI
device, roi1, roi_hierarchy = pcv.define_roi(img, 'circle', device, None,
'default', args.debug, True,
0, 0, -50, -50)
# Decide which objects to keep
device, roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(
img, 'partial', roi1, roi_hierarchy, id_objects, obj_hierarchy, device,
args.debug)
# Object combine kept objects
device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3,
device, args.debug)
############## Analysis ################
# Find shape properties, output shape image (optional)
device, shape_header, shape_data, shape_img = pcv.analyze_object(
img, args.image, obj, mask, device, args.debug,
args.outdir + '/' + filename)
# Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
device, color_header, color_data, norm_slice = pcv.analyze_color(
img, args.image, kept_mask, 256, device, args.debug, 'all', 'rgb', 'v',
'img', 300, args.outdir + '/' + filename)
# Output shape and color data
pcv.print_results(args.image, shape_header, shape_data)
pcv.print_results(args.image, color_header, color_data)
def main(): # Get options args = options() # Read image img, path, filename = pcv.readimage(args.image) #roi = cv2.imread(args.roi) # Pipeline step device = 0 # Convert RGB to HSV and extract the Saturation channel device, s = pcv.rgb2gray_lab(img, 'l', device, args.debug) # Threshold the Saturation image device, s_thresh = pcv.binary_threshold(s, 100, 255, 'light', device, args.debug) # Median Filter device, s_mblur = pcv.median_blur(s_thresh, 0, device, args.debug) device, s_cnt = pcv.median_blur(s_thresh, 0, device, args.debug) # Fill small objects #device, s_fill = pcv.fill(s_mblur, s_cnt, 0, device, args.debug) ## Convert RGB to LAB and extract the Blue channel #device, b = pcv.rgb2gray_lab(img, 'b', device, args.debug) ## Threshold the blue image #device, b_thresh = pcv.binary_threshold(b, 145, 255, 'light', device, args.debug) #device, b_cnt = pcv.binary_threshold(b, 145, 255, 'light', device, args.debug) # # Fill small objects #device, b_fill = pcv.fill(b_thresh, b_cnt, 10, device, args.debug) ## Join the thresholded saturation and blue-yellow images #device, bs = pcv.logical_and(s_mblur, b_cnt, device, args.debug) # Apply Mask (for vis images, mask_color=white) device, masked = pcv.apply_mask(img, s_cnt, 'white', device, args.debug) # Convert RGB to LAB and extract the Green-Magenta and Blue-Yellow channels device, masked_a = pcv.rgb2gray_lab(masked, 'a', device, args.debug) device, masked_b = pcv.rgb2gray_lab(masked, 'b', device, args.debug) # Threshold the green-magenta and blue images device, maskeda_thresh = pcv.binary_threshold(masked_a, 127, 255, 'dark', device, args.debug) device, maskedb_thresh = pcv.binary_threshold(masked_b, 128, 255, 'light', device, args.debug) # Join the thresholded saturation and blue-yellow images (OR) device, ab = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug) # Fill small objects device, ab_fill = pcv.fill(ab, ab_cnt, 20, device, args.debug) # Apply mask (for vis images, mask_color=white) device, masked2 = pcv.apply_mask(masked, ab_fill, 'white', device, args.debug) # Identify objects device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug) # Define ROI device, roi1, roi_hierarchy= pcv.define_roi(img,'rectangle', device, None, 'default', args.debug,True, 25, 25,-10,-25) # Decide which objects to keep device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug) # Object combine kept objects device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug) # ############### Analysis ################ # Find shape properties, output shape image (optional) device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename) # Shape properties relative to user boundary line (optional) device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 25, device,args.debug,args.outdir+'/'+filename) # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional) device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename) # Output shape and color data pcv.print_results(args.image, shape_header, shape_data) pcv.print_results(args.image, color_header, color_data) pcv.print_results(args.image, boundary_header, boundary_data)