ScRPS19a_PacI-ScRKI1-ScTPI1_PacI ScTPI1_PacI-CiGXF1-ScENO2_PacI ''' from pydna import * from pydna_helper.load_my_primers import primer from pydna_helper import gb from Bio.SeqUtils.CheckSum import seguid from pygenome import sg as sgd ScPDC1tp = pcr([primer[413],primer[625]], Dseqrecord(sgd.promoter("PDC1"))) ScRPS19atp = pcr([primer[538],primer[539]], Dseqrecord(sgd.promoter("RPS19a"))) ScRPS19btp = pcr([primer[537],primer[536]], Dseqrecord(sgd.promoter("RPS19b"))) ScTPI1tp = pcr([primer[419],primer[621]], Dseqrecord(sgd.promoter("TPI1"))) ScENO2tp = pcr([primer[411],primer[452]], Dseqrecord(sgd.intergenic_sequence("YHR172W", "YHR174W"))) # load pCAPs and pCAPs-pSU0 as dsdna objects from pYPKa import pYPKa from Bio.Restriction import EcoRV, ZraI, AjiI pYPKa_ZraI = pYPKa.cut(ZraI).pop() pYPKa_AjiI = pYPKa.cut(AjiI).pop() pYPKa_EcoRV = pYPKa.cut(EcoRV).pop() pCAPs_ZraI_ScPDC1tp = (pYPKa_ZraI + ScPDC1tp).looped() pCAPs_ZraI_ScRPS19atp = (pYPKa_ZraI + ScRPS19atp).looped() pCAPs_EcoRV_ScRPS19atp = (pYPKa_EcoRV + ScRPS19atp).looped()
# coding: utf-8 from pydna import * from pydna_helper.load_my_primers import primer, new_primer from pydna_helper import gb, ape from Bio.SeqUtils.CheckSum import seguid from pyeast import saccharomyces_cerevisiae_genome as sc from pygenome import sg as sgd ScTEF1tp = pcr([primer[417],primer[626]], Dseqrecord(sgd.promoter("TEF1"))) ScTDH3tp = pcr([primer[415],primer[623]], Dseqrecord(sgd.promoter("TDH3"))) ScPGI1tp = pcr([primer[549],primer[622]], Dseqrecord(sgd.intergenic_sequence("YBR197C", "PGI1"))) ScFBA1tp = pcr([primer[409],primer[624]], Dseqrecord(sgd.promoter("FBA1"))) ScPDC1tp = pcr([primer[413],primer[625]], Dseqrecord(sgd.promoter("PDC1"))) # load pCAPs and pCAPs-pSU0 as dsdna objects pCAPs = read("/home/bjorn/Dropbox/wikidata/pCAPs-AjiI.wiki") from Bio.Restriction import EcoRV, ZraI, AjiI pCAPs_ZraI = pCAPs.cut(ZraI).pop() pCAPs_AjiI = pCAPs.cut(AjiI).pop() pCAPs_EcoRV = pCAPs.cut(EcoRV).pop() pCAPs_ZraI_ScTEF1tp = (pCAPs_ZraI + ScTEF1tp).looped() pCAPs_ZraI_ScTDH3tp = (pCAPs_ZraI + ScTDH3tp).looped() pCAPs_EcoRV_ScTDH3tp = (pCAPs_EcoRV + ScTDH3tp).looped()