def test_FRM(self):
import swig_frm
from sh_alignment.frm import frm_align
from pytom_volume import vol, rotate, shift
from pytom.basic.structures import Rotation, Shift
from pytom.tools.maths import rotation_distance
v = vol(32,32,32)
v.setAll(0)
vMod = vol(32,32,32)
vRot = vol(32,32,32)
v.setV(1,10,10,10)
v.setV(1,20,20,20)
v.setV(1,15,15,15)
v.setV(1,7,21,7)
rotation = Rotation(10,20,30)
shiftO = Shift(1,-3,5)
rotate(v,vRot,rotation.getPhi(),rotation.getPsi(),rotation.getTheta())
shift(vRot,vMod,shiftO.getX(),shiftO.getY(),shiftO.getZ())
pos, ang, score = frm_align(vMod, None, v, None, [4, 64], 10)
rotdist = rotation_distance(ang1=rotation, ang2=ang)
diffx = shiftO[0] - (pos[0] - 16)
diffy = shiftO[1] - (pos[1] - 16)
diffz = shiftO[2] - (pos[2] - 16)
self.assertTrue( rotdist < 5., msg='Rotations are different')
self.assertTrue( diffx < .5, msg='x-difference > .5')
self.assertTrue( diffy < .5, msg='y-difference > .5')
self.assertTrue( diffz < .5, msg='z-difference > .5')
def stdUnderMask(volume, mask, p, meanV):
"""
stdUnderMask: calculate the std volume under the given mask
@param volume: input volume
@type volume: L{pytom_volume.vol}
@param mask: mask
@type mask: L{pytom_volume.vol}
@param p: non zero value numbers in the mask
@type p: L{int}
@param meanV: mean volume under mask, which should already been caculated
@type meanV: L{pytom_volume.vol}
@return: the calculated std volume under mask
@rtype: L{pytom_volume.vol}
@author: Yuxiang Chen
"""
from pytom.basic.fourier import fft, ifft, iftshift
from pytom_volume import vol, power, limit
copyV = vol(volume.sizeX(), volume.sizeY(), volume.sizeZ())
copyV.copyVolume(volume)
power(copyV, 2) #calculate the square of the volume
copyMean = vol(meanV.sizeX(), meanV.sizeY(), meanV.sizeZ())
copyMean.copyVolume(meanV)
power(copyMean, 2)
result = meanUnderMask(copyV, mask, p) - copyMean
# from pytom_volume import abs
# abs(result)
limit(result, 1e-09, 1, 0, 0, True, False) # this step is needed to set all those value (close to 0) to 1
power(result, 0.5)
return result
def distance(p, ref, freq, mask, binning):
from pytom.basic.correlation import nxcc
from pytom_volume import vol, initSphere, read, pasteCenter
from pytom.basic.filter import lowpassFilter
from pytom.basic.transformations import resize
v = p.getTransformedVolume(binning)
w = p.getWedge()
r = ref.getVolume()
a = lowpassFilter(w.apply(v, p.getRotation().invert()), freq)[0]
b = lowpassFilter(w.apply(r, p.getRotation().invert()), freq)[0]
if not mask:
mask = vol(r)
initSphere(mask,
r.sizeX() // 2 - 3, 3, 0,
r.sizeX() // 2,
r.sizeY() // 2,
r.sizeZ() // 2)
else:
#THE MASK is binning (sampled every n-points). This does lead to a reduction of the smoothing of the edges.
maskBin = read(mask, 0, 0, 0, 0, 0, 0, 0, 0, 0, binning, binning,
binning)
if a.sizeX() != maskBin.sizeX() or a.sizeY() != maskBin.sizeY(
) or a.sizeZ() != maskBin.sizeZ():
mask = vol(a.sizeX(), a.sizeY(), a.sizeZ())
mask.setAll(0)
pasteCenter(maskBin, mask)
else:
mask = maskBin
s = nxcc(a, b, mask)
d2 = 2 * (1 - s)
return d2
def theta_vol_projection(vol_src, theta):
from pytom_volume import vol
from pytom_volume import rotate
from pytom_volume import subvolume
from pytom_volume import sum
vol_src_dim_x = vol_src.sizeX()
vol_src_dim_y = vol_src.sizeY()
vol_src_dim_z = vol_src.sizeZ()
vol_dst = vol(vol_src_dim_x, vol_src_dim_y, vol_src_dim_z)
vol_dst.setAll(0.0)
rotate(vol_src, vol_dst, 270, 90, theta)
vol_img = vol(vol_src_dim_x, vol_src_dim_y, 1)
vol_img.setAll(0.0)
for i in range(vol_src_dim_x):
for j in range(vol_src_dim_y):
vol_img.setV(sum(subvolume(vol_dst, i, j, 0, 1, 1, vol_src_dim_z)),
i, j, 0)
return vol_img
def rotateWeighting(weighting, z1, z2, x, mask=None, isReducedComplex=None, returnReducedComplex=False, binarize=False):
"""
rotateWeighting: Rotates a frequency weighting volume around the center. If the volume provided is reduced complex, it will be rescaled to full size, ftshifted, rotated, iftshifted and scaled back to reduced size.
@param weighting: A weighting volume
@type weighting: L{pytom_volume.vol}
@param z1: Z1 rotation angle
@type z1: float
@param z2: Z2 rotation angle
@type z2: float
@param x: X rotation angle
@type x: float
@param mask:=None is there a rotation mask? A mask with all = 1 will be generated otherwise. Such mask should be \
provided anyway.
@type mask: L{pytom_volume.vol}
@param isReducedComplex: Either set to True or False. Will be determined otherwise
@type isReducedComplex: bool
@param returnReducedComplex: Return as reduced complex? (Default is False)
@type returnReducedComplex: bool
@param binarize: binarize weighting
@type binarize: bool
@return: weight as reduced complex volume
@rtype: L{pytom_volume.vol_comp}
"""
from pytom_volume import vol, limit, vol_comp
from pytom_volume import rotate
assert type(weighting) == vol or type(weighting) == vol_comp, "rotateWeighting: input neither vol nor vol_comp"
isReducedComplex = isReducedComplex or int(weighting.sizeX()/2)+1 == weighting.sizeZ();
if isReducedComplex:
#scale weighting to full size
from pytom_fftplan import fftShift
from pytom_volume import reducedToFull
weighting = reducedToFull(weighting)
fftShift(weighting, True)
if not mask:
mask = vol(weighting.sizeX(),weighting.sizeY(),weighting.sizeZ())
mask.setAll(1)
weightingRotated = vol(weighting.sizeX(),weighting.sizeY(),weighting.sizeZ())
rotate(weighting,weightingRotated,z1,z2,x)
weightingRotated = weightingRotated * mask
if returnReducedComplex:
from pytom_fftplan import fftShift
from pytom_volume import fullToReduced
fftShift(weightingRotated,True)
returnVolume = fullToReduced(weightingRotated)
else:
returnVolume = weightingRotated
if binarize:
limit(returnVolume,0.5,0,0.5,1,True,True)
return returnVolume
def setUp(self):
"""set up"""
# a point somewhere
self.myVol = vol(256, 256, 1)
self.myVol.setAll(0.)
self.myVol.setV(1., 200, 128, 0)
self.r = array([200 - 128, 0])
self.origin = vol(256, 256, 1)
self.origin.setAll(0.)
self.origin.setV(1., 128, 128, 0)
self.m = 0.8
def writeCroppedParticles(particleListName, output, center, cubesize):
"""
@param particleListName: name of particle list
@type particleListName: str
@param output: Name of output particles
@type output: str
@param center: center of output particles in template orientation
@type center: list
@param cubesize: Size of output particles in pixel
@type cubesize: int
"""
from pytom.basic.structures import ParticleList, Particle, Shift
from pytom_volume import transformSpline as transform
from pytom_volume import subvolume, vol
pl = ParticleList()
pl.fromXMLFile(filename=particleListName)
#copy particle list for list of cropped particles
pl_new = pl.copy()
pvol = pl[0].getVolume()
sizeX = pvol.sizeX()
sizeY = pvol.sizeY()
sizeZ = pvol.sizeZ()
pvol_ali = vol(sizeX, sizeY, sizeZ)
subV = vol(cubesize, cubesize, cubesize)
sub_startX = center[0]-cubesize/2
sub_startY = center[1]-cubesize/2
sub_startZ = center[2]-cubesize/2
if (sub_startX < 0) or (sub_startY < 0) or (sub_startZ < 0):
raise ValueError('cubesize too large :(')
for (ipart, part) in enumerate(pl):
pvol_ali.setAll(0)
subV.setAll(0)
pvol = part.getVolume()
rot = part.getRotation()
rotinvert = rot.invert()
shiftV = part.getShift()
transform(pvol, pvol_ali, rotinvert[0], rotinvert[1], rotinvert[2],
sizeX/2, sizeY/2, sizeZ/2, -shiftV[0], -shiftV[1], -shiftV[2], 0, 0, 0)
# box out subvolume
subV = subvolume(pvol_ali, sub_startX, sub_startY, sub_startZ, cubesize, cubesize, cubesize)
transform(subV, subV, rot[0], rot[1], rot[2], cubesize/2, cubesize/2, cubesize/2, 0, 0, 0, 0, 0, 0)
fname = part.getFilename()
idx = fname.split('_')[-1].split('.')[0]
nfname = output+'_'+idx+'.em'
print("write file " + nfname)
subV.write(nfname)
pl_new[ipart].setFilename(newFilename=nfname)
pl_new[ipart].setShift(shift=Shift(0,0,0))
return pl_new
def sum_sub_pl(self, pl, name_prefix):
"""This is a sub-routine for average_sub_pl.
"""
from pytom_volume import vol
from pytom_volume import transformSpline as transform
from pytom.basic.normalise import mean0std1
result = None
wedgeSum = None
for p in pl:
particle = p.getVolume()
mean0std1(particle)
wedgeInfo = p.getWedge()
if result is None:
sizeX = particle.sizeX()
sizeY = particle.sizeY()
sizeZ = particle.sizeZ()
newParticle = vol(sizeX, sizeY, sizeZ)
# make consistent for python3
centerX = sizeX // 2
centerY = sizeY // 2
centerZ = sizeZ // 2
result = vol(sizeX, sizeY, sizeZ)
result.setAll(0)
wedgeSum = wedgeInfo.returnWedgeVolume(sizeX, sizeY, sizeZ)
wedgeSum.setAll(0)
# create wedge weighting
rotation = p.getRotation()
wedge = wedgeInfo.returnWedgeVolume(sizeX, sizeY, sizeZ, False,
rotation.invert())
wedgeSum = wedgeSum + wedge
# shift and rotate particle
shift = p.getShift()
newParticle.setAll(0)
transform(particle, newParticle, -rotation[1], -rotation[0],
-rotation[2], centerX, centerY, centerZ, -shift[0],
-shift[1], -shift[2], 0, 0, 0)
result = result + newParticle
# write them back to disk
result.write(name_prefix + '-PreWedge.em')
wedgeSum.write(name_prefix + '-WedgeSumUnscaled.em')
def showRotations(self,volume,directory,mask=None):
"""
showRotations: Will store x,y,z slice through volume after each rotation was applied
"""
from pytom.tools.toImage import volumeToPNG
from pytom.tools.files import dump2TextFile
from pytom_volume import vol,rotateSpline
from pytom.tools.files import getPytomPath,readStringFile
from pytom.frontend.htmlDefines import headResponse200,copyright
pytomPath = getPytomPath()
cssDefines = readStringFile(pytomPath + '/frontend/htmlDefines/CSS.css')
html = '<html>' + cssDefines
html = html + '\n<title>PyTom Alignment</title>\n'
html = html + '<body><center><h1 type="Main">List of Rotations</h1></center>\n'
html = html + '</br></br></br></br></br></br></br></br><center>\n'
html = html + '<table>\n'
html = html + '<tr><td>Z1 (Phi) Z2 (Psi) X(Theta)</td><td>x-Slice</td><td>y-Slice</td><td>z-Slice</td></tr>'
rotated = vol(volume.sizeX(),volume.sizeY(),volume.sizeZ())
if not mask:
mask = vol(volume.sizeX(),volume.sizeY(),volume.sizeZ())
mask.setAll(1)
volume = volume * mask
self.reset()
rotation = self.nextRotation()
rotationCounter = 0
while not rotation == [None,None,None]:
rotateSpline(volume,rotated,rotation[0],rotation[1],rotation[2])
html = html + '<tr><td>' + str(rotation[0]) + ' ' + str(rotation[1]) + ' ' + str(rotation[2]) +'</td>/n'
volumeToPNG(rotated,directory + str(rotationCounter) + '-x.png',rotated.sizeX()/2,'x')
html = html + '<td><img src="' + directory + str(rotationCounter) + '-x.png"/></td>'
volumeToPNG(rotated,directory + str(rotationCounter) + '-y.png',rotated.sizeY()/2,'y')
html = html + '<td><img src="' + directory + str(rotationCounter) + '-y.png"/></td>'
volumeToPNG(rotated,directory + str(rotationCounter) + '-z.png',rotated.sizeZ()/2,'z')
html = html + '<td><img src="' + directory + str(rotationCounter) + '-z.png"/></td>'
rotation = self.nextRotation()
rotationCounter = rotationCounter + 1
html = html + '</body></html>'
dump2TextFile(directory + 'rotations.html',html)
def create_bfactor_vol(size, ps, bfactor, FSC=None, apply_range=None):
"""Create a B-factor volume in Frequency space.
@param size: The size of the volume, assuming it is a cube
@param ps: The pixel size in angstrom
@param bfactor: B factor
@param FSC: Fourier Shell Correlation
@param apply_range: The apply range (also in angstrom) of the B factor correction
"""
if FSC is None:
FSC = np.ones(size / 2)
# transfer the pixel size to angstrom
x = (ps * size) / np.arange(1, size / 2 + 1, 1) # starts from 1!
if apply_range is None:
apply_range_pixel = [1, size / 2] # starts from 1!
else:
assert apply_range[0] > apply_range[1]
apply_range_pixel = [
size * ps / apply_range[0], size * ps / apply_range[1]
]
# create the FSC weight
FSC_weight = np.sqrt((2 * np.array(FSC)) / (1 + np.array(FSC)))
# calculate the decay function
decay = FSC_weight * np.exp(-bfactor / (np.power(x, 2) * 4))
# make the decay volume
v = sph2cart(decay, size)
# transfer to the volume format and multiple with the mask
from pytom_volume import vol, initSphere
from pytom_numpy import npy2vol
vv = npy2vol(np.array(v, dtype='float32', order='F'), 3)
if apply_range_pixel[0] == 1:
mask = vol(size, size, size)
initSphere(mask, apply_range_pixel[1] - 1, 0, 0, size / 2, size / 2,
size / 2) # minus 1 to make it consistent with python
else:
mask1 = vol(size, size, size)
mask2 = vol(size, size, size)
initSphere(mask1, apply_range_pixel[0] - 1, 0, 0, size / 2, size / 2,
size / 2)
initSphere(mask2, apply_range_pixel[1] - 1, 0, 0, size / 2, size / 2,
size / 2)
mask = mask2 - mask1
return vv * mask
def setUp(self):
from helper_functions import create_RandomParticleList
from pytom.tompy.io import read_size
from pytom_volume import vol, initSphere
self.reffile = './testData/ribo.em'
self.pl_filename = 'pl.xml'
self.pdir = './testparticles'
self.pl = create_RandomParticleList(reffile=self.reffile,
pl_filename=self.pl_filename,
pdir=self.pdir,
nparticles=10)
# set parameters for GLocal
self.settings = {}
self.settings["binning"] = 4
self.settings["niteration"] = 1
self.settings["mask"] = './testData/ribo_mask.em'
dims = read_size(self.reffile)
maskvol = vol(int(dims[0]), int(dims[1]), int(dims[2]))
initSphere(maskvol, 30, 5, 0, int(dims[0] / 2), int(dims[1] / 2),
int(dims[2] / 2))
maskvol.write(self.settings["mask"])
self.settings["destination"] = './'
#self.settings["score"] = 'nxcf'
self.settings["score"] = 'flcf'
self.settings["pixelsize"] = 2.
self.settings["diameter"] = 250.
self.settings["job"] = './myGLocal.xml'
def rampFilter(sizeX, sizeY):
"""
rampFilter: Generates the weighting function required for weighted backprojection - y-axis is tilt axis
@param sizeX: size of weighted image in X
@param sizeY: size of weighted image in Y
@return: filter volume
"""
from pytom_volume import vol
from math import exp
import time
s = time.time()
centerX = sizeX // 2
centerY = sizeY // 2
sizeY = (sizeY // 2) + 1
Ny = sizeX // 2
filter_vol = vol(sizeX, sizeY, 1)
filter_vol.setAll(0.0)
for i in range(sizeX):
distX = abs(float(i - centerX))
ratio = distX / Ny
for j in range(sizeY):
filter_vol.setV(ratio, i, j, 0)
print('ramp filter takes: ', time.time() - s, sizeX, sizeY)
return filter_vol
def rotationDistanceMatrix(self):
"""
rotationDistanceMatrix: Generate a matrix of rotation distances. The
distance is determined by L{pytom.angles.quaternions.Quaternion.distance}.
@return: L{pytom_volume.vol} of rotation distances
@author: Thomas Hrabe
"""
from pytom_volume import vol
from pytom.tools.maths import rotation_distance
numberOfRotations = len(self)
distanceMatrix = vol(numberOfRotations,numberOfRotations,1)
distanceMatrix.setAll(0)
for i in range(len(self)):
if i < len(self):
for j in range(i+1,len(self)):
ri = self[i]
rj = self[j]
d = rotation_distance(ri,rj)
distanceMatrix(d,i,j,0)
distanceMatrix(d,j,i,0)
distanceMatrix(0,i,i,0)
return distanceMatrix
def average(self, mask=None):
"""
average Image Stack
@param mask: mask is multiplied with average if specified
@type mask: L{pytom_volume.vol}
@return: average
@rtype: L{ptom_volume.vol}
"""
from pytom.basic.transformations import general_transform2d
if self.averageData == None:
self.averageData = vol(self.dimX, self.dimY, 1)
self.averageData.setAll(0.)
for ii in range(0, len(self.images)):
self.imageCopies[ii].data = general_transform2d(
v=self.images[ii].data,
rot=-self.images[ii].rotation,
shift=[-self.images[ii].shiftX, -self.images[ii].shiftY],
scale=1.,
order=[2, 1, 0],
crop=True)
self.averageData = self.averageData + self.imageCopies[ii].data
if mask:
self.averageData = self.averageData * mask
return self.averageData
def toEMFile(self,filename, inputIsRadians=False):
"""
toEMFile: Will save all rotations to EM file. The EM file can be read out by L{pytom.angles.fromFile.AngleListFromEM}
@param filename: Name of file to save to
@param inputIsRadians:
"""
from pytom_volume import vol
no = self.numberRotations()
anglesVolume = vol(3,no,1)
rotations = self.getAllRotations()
i = 0
for rotation in rotations:
if rotation == [None, None, None]:
break
if inputIsRadians:
anglesVolume.setV(rotation[0],0,i,0)
anglesVolume.setV(rotation[1],1,i,0)
anglesVolume.setV(rotation[2],2,i,0)
else:
from pytom.angles.angle import deg2rad
anglesVolume.setV(rotation[0]*deg2rad,0,i,0)
anglesVolume.setV(rotation[1]*deg2rad,1,i,0)
anglesVolume.setV(rotation[2]*deg2rad,2,i,0)
i = i+1
anglesVolume.write(filename)
def powerspectrum(volume):
"""
compute power spectrum of a volume
@param volume: input volume
@type volume: L{pytom_volume.vol}
@return: power spectrum of vol
@rtype: L{pytom_volume.vol}
@author: FF
"""
from pytom.basic.fourier import fft, ftshift
from pytom_volume import vol
from pytom_volume import reducedToFull
fvol = ftshift(reducedToFull(fft(volume)),inplace=False)
nx=fvol.sizeX()
ny=fvol.sizeY()
nz=fvol.sizeZ()
ps = vol(nx,ny,nz)
sf = 1./(nx*ny*nz)
for ix in range(0,nx):
for iy in range(0,ny):
for iz in range(0,nz):
temp = fvol.getV(ix,iy,iz)
temp = temp*temp.conjugate()*sf
ps.setV(float(temp.real),ix,iy,iz)
return ps
def toMotif(self, filename):
"""
toMotif:
@param filename:
@type filename: L{str}
@todo: add unit test
"""
from pytom_volume import vol
l = self.len()
motif = vol(20, l, 1)
motif.setAll(0)
for i in range(l):
#correlation coefficient
motif.setV(1, 0, i, 0)
motif.setV(i + 1, 3, i, 0)
res = self._alignmentList[i]
shift = res.getShift()
motif.setV(shift[0], 10, i, 0)
motif.setV(shift[1], 11, i, 0)
motif.setV(shift[2], 12, i, 0)
rotation = res.getRotation()
motif.setV(rotation[0], 16, i, 0)
motif.setV(rotation[1], 17, i, 0)
motif.setV(rotation[2], 18, i, 0)
motif.write(filename)
def readSpider(filename):
"""
readSpider: Reads a spider file and returns a volume
@param filename: The file name
@return: L{pytom_volume.vol}
"""
if not checkFileExists(filename):
raise IOError('File ' + filename + ' not found!')
from pytom_volume import vol
import struct
from math import ceil
fileHandle = open(filename,'rb')
z = int(struct.unpack('f',fileHandle.read(4))[0]) #nslice
x = int(struct.unpack('f',fileHandle.read(4))[0]) #nrows
fileHandle.seek(44)
y = int(struct.unpack('f',fileHandle.read(4))[0]) #nsam
volume = vol(x,y,z)
fileHandle.seek(int(ceil(256.0/float(y)) * y * 4))
for z in range(volume.sizeZ()):
for y in range(volume.sizeY()):
for x in range(volume.sizeX()):
value = float(struct.unpack('f',fileHandle.read(4))[0])
volume(value,x,y,z)
fileHandle.close()
return volume
def fourierFilterShift_ReducedComplex(filter):
"""
fourierFilterShift: NEEDS Documentation
@param filter: NEEDS Documentation
"""
from pytom_volume import vol
widthX = filter.sizeX()
centerX = filter.sizeX()//2
boxX = filter.sizeX()//2
widthY = filter.sizeY()
centerY = filter.sizeY()//2
boxY = filter.sizeY()//2
shifted_filter = vol(widthX, widthY, 1)
shifted_filter.setAll(0.0)
for i in range(widthX):
rx = (boxX-i)%widthX
for j in range(widthY):
ry = (boxY-j)%widthY
shifted_filter.setV(filter.getV(i, j, 0), rx, widthY-j-1, 0)
return shifted_filter
def circleFilter(sizeX,sizeY, radiusCutoff):
"""
circleFilter: NEEDS Documentation
@param sizeX: NEEDS Documentation
@param sizeY: NEEDS Documentation
@param radiusCutoff: NEEDS Documentation
"""
from pytom_volume import vol
centerX = sizeX//2
centerY = sizeY//2
sizeY = (sizeY//2) +1
filter_vol = vol(sizeX, sizeY, 1)
filter_vol.setAll(0.0)
for i in range(sizeX):
for j in range(sizeY):
radius = ((i-centerX)*(i-centerX)+(j-centerY)*(j-centerY))**0.5
if radius <= radiusCutoff:
filter_vol.setV(1.0, i, j, 0)
return filter_vol
def test_resize2D(self):
"""
test re-sizing in Fourier space
"""
from pytom.basic.transformations import resize
from pytom_volume import vol
from pytom.basic.fourier import fft
dim = 32
px = 11
py = 19
scf = dim * dim
myVol = vol(dim, dim, 1)
myVol.setAll(0.)
myVol.setV(1., px, py, 0)
#fmyVol = fft(myVol)
(resizeVol, resizefVol) = resize(volume=myVol,
factor=2.,
interpolation='Fourier')
resizeVol.write('test1.em')
ftresizeVol = fft(data=resizeVol)
for ix in range(resizefVol.sizeX()):
for iy in range(resizefVol.sizeY()):
diff = ftresizeVol.getV(
ix, iy, 0) - scf * 4 * resizefVol.getV(ix, iy, 0)
self.assertTrue(expr=abs(diff) < .05,
msg="inconsistency FFT/IFFT for magnification")
(resizeVol, resizefVol) = resize(volume=resizeVol,
factor=.5,
interpolation='Fourier')
from pytom_volume import variance
diff = myVol - resizeVol
self.assertTrue(expr=variance(diff, False) < .0000001,
msg="2D image before and after rescales differs")
def add(volume, SNR=1):
"""
add Adds white noise to volume
@param volume: A volume
@param SNR: Signal to Noise ratio of result
@type SNR: int or float > 0
@return: Volume containing noise with SNR == SNR
@author: Thomas Hrabe
"""
if (SNR < 0):
return volume
from math import sqrt
from pytom_volume import vol, mean, variance, gaussianNoise
m = mean(volume)
s = sqrt(variance(volume, False) / SNR) # SNR = Var(signal) / Var(noise)
# s = sqrt(variance(volume,False)/SNR)-variance(volume,False)
#
# if s<0:
# return volume
# elif s==0:
# s =1
noise = vol(volume.sizeX(), volume.sizeY(), volume.sizeZ())
gaussianNoise(noise, m, s) # s is actually the std
result = volume + noise
return result
def initSphere(cubeSize,
radius,
smoothing=0,
centerX=None,
centerY=None,
centerZ=None):
"""
initSphere: Initilizes a volume with a sphere
@param cubeSize: The size of the whole volume
@param radius: Radius of the sphere
@param smoothing: Smoothing at the edges of the sphere
@param centerX: Center of shpere along X axis
@param centerY: Center of shpere along Y axis
@param centerZ: Center of shpere along Z axis
"""
from pytom_volume import vol, initSphere
sphere = vol(cubeSize, cubeSize, cubeSize)
sphere.setAll(0)
if centerX is None:
centerX = cubeSize / 2 - 1
if centerY is None:
centerY = cubeSize / 2 - 1
if centerZ is None:
centerZ = cubeSize / 2 - 1
initSphere(sphere, radius, smoothing, 0, centerX, centerY, centerZ)
return sphere
def rampFilter( sizeX, sizeY, crowtherFreq=None):
"""
rampFilter: Generates the weighting function required for weighted backprojection - y-axis is tilt axis
@param sizeX: size of weighted image in X
@param sizeY: size of weighted image in Y
@return: filter volume
"""
from pytom_volume import vol
from math import exp
centerX = sizeX//2
centerY = sizeY//2
sizeY = (sizeY//2) +1
if crowtherFreq is None:
Ny = sizeX//2
else:
Ny = crowtherFreq
filter_vol = vol(sizeX, sizeY, 1)
filter_vol.setAll(0.0)
for i in range(sizeX):
distX = abs(float(i-centerX))
ratio = min(1, distX/Ny)
for j in range(sizeY):
filter_vol.setV(ratio, i, j, 0)
return filter_vol
def hanning_taper(volume1, avg):
import math
sizex = volume1.sizeX()
sizey = volume1.sizeY()
sizez = volume1.sizeZ()
cx0 = math.pi * 2.0 /(sizex - 1)
cx1 = math.pi * 2.0 /(sizey - 1)
cx2 = math.pi * 2.0 /(sizez - 1)
volume2 = vol(sizex,sizey,sizez)
volume2.setAll(0)
for zz in xrange(0, sizez):
han2 = 0.5 * (1.0 - math.cos(cx2 * zz))
for yy in xrange(0, sizey):
han1 = 0.5 * (1.0 - math.cos(cx1 * yy))
for xx in xrange(0, sizex):
han0 = 0.5 * (1.0 - math.cos(cx0 * xx))
vol_han = han0 * han1 * han2
value2 = (volume1(xx,yy,zz) - avg)*vol_han + avg
volume2.setV(round(value2,6),xx,yy,zz)
del cx0,cx1,cx2,sizex,sizey,sizez,han0,han1,han2,value2,vol_han
return volume2
def power(volume, exponent, inplace=False):
"""
power: Pixelwise power
@param volume: The volume
@type volume: L{pytom_volume.vol}
@param exponent: The exponent
@type exponent: L{float}
@param inplace: Perform power inplace? Default is False
@type inplace: L{bool}
@return: volume
@rtype: L{pytom_volume.vol}
"""
if inplace:
from pytom_volume import power
power(volume, exponent)
else:
#return new volume object
from pytom_volume import vol, power
volume2 = vol(volume.sizeX(), volume.sizeY(), volume.sizeZ())
volume2.copyVolume(volume)
power(volume2, exponent)
return volume2
def frm_proxy(p, ref, freq, offset, binning, mask):
from pytom_volume import read, pasteCenter, vol
from pytom.basic.transformations import resize
from pytom.basic.structures import Shift, Rotation
from sh_alignment.frm import frm_align
import time
v = p.getVolume(binning)
if mask.__class__ == str:
maskBin = read(mask, 0, 0, 0, 0, 0, 0, 0, 0, 0, binning, binning,
binning)
if v.sizeX() != maskBin.sizeX() or v.sizeY() != maskBin.sizeY(
) or v.sizeZ() != maskBin.sizeZ():
mask = vol(v.sizeX(), v.sizeY(), v.sizeZ())
mask.setAll(0)
pasteCenter(maskBin, mask)
else:
mask = maskBin
pos, angle, score = frm_align(v, p.getWedge(), ref.getVolume(), None,
[4, 64], freq, offset, mask)
return (Shift([
pos[0] - v.sizeX() // 2, pos[1] - v.sizeY() // 2,
pos[2] - v.sizeZ() // 2
]), Rotation(angle), score, p.getFilename())
def calculate_difference_map_proxy(r1, band1, r2, band2, mask, focus_mask, binning, iteration, sigma, threshold, outdir='./'):
from pytom_volume import read, vol, pasteCenter
from pytom.basic.structures import Particle, Mask
import os
from pytom.basic.transformations import resize
v1 = r1.getVolume()
v2 = r2.getVolume()
if mask:
maskBin = read(mask, 0,0,0,0,0,0,0,0,0, binning, binning, binning)
if v1.sizeX() != maskBin.sizeX() or v1.sizeY() != maskBin.sizeY() or v1.sizeZ() != maskBin.sizeZ():
mask = vol(v1.sizeX(), v1.sizeY(), v1.sizeZ())
mask.setAll(0)
pasteCenter(maskBin, mask)
else:
mask = maskBin
else:
mask = None
if focus_mask:
focusBin = read(focus_mask, 0,0,0,0,0,0,0,0,0, binning, binning, binning)
if v1.sizeX() != focusBin.sizeX() or v1.sizeY() != focusBin.sizeY() or v1.sizeZ() != focusBin.sizeZ():
focus_mask = vol(v1.sizeX(), v1.sizeY(), v1.sizeZ())
focus_mask.setAll(0)
pasteCenter(focusBin, focus_mask)
else:
focus_mask = focusBin
else:
focus_mask = None
if not focus_mask is None and not mask is None:
if mask.sizeX() != focus_mask.sizeX():
raise Exception('Focussed mask and alignment mask do not have the same dimensions. This cannot be correct.')
(dmap1, dmap2) = calculate_difference_map(v1, band1, v2, band2, mask, focus_mask, True, sigma, threshold)
fname1 = os.path.join(outdir, 'iter'+str(iteration)+'_dmap_'+str(r1.getClass())+'_'+str(r2.getClass())+'.em')
dmap1.write(fname1)
fname2 = os.path.join(outdir, 'iter'+str(iteration)+'_dmap_'+str(r2.getClass())+'_'+str(r1.getClass())+'.em')
dmap2.write(fname2)
dp1 = Particle(fname1)
dp1.setClass(r1.getClass())
dp2 = Particle(fname2)
dp2.setClass(r2.getClass())
return (dp1, dp2)
def create_bfactor_restore_vol(size, ps, bfactor, FSC=None, apply_range=None):
from pytom_volume import vol
v = create_bfactor_vol(size, ps, bfactor, FSC, apply_range)
unit = vol(v)
unit.setAll(1)
restore = unit / v
return restore
def applySymmetryToVolume(volume,symmetryObject,wedgeInfo):
"""
applySymmetryToVolume
@deprecated: use L{pytom.basic.structures.Symmetry.apply} instead!
"""
from pytom_volume import read,rotate,shift,vol,initSphere,complexDiv
from pytom_freqweight import weight
from pytom.basic.fourier import fft,ifft,ftshift
from pytom.basic.filter import filter
from pytom.alignment.structures import ExpectationResult
from pytom.basic.structures import Reference,Symmetry
from pytom.tools.maths import epsilon
if not volume.__class__ == vol:
raise Exception('You must provide a volume as first parameter to applySymmetryToObject')
if not symmetryObject.__class__ == Symmetry:
raise Exception('You must provide a Symmetry object as second parameter to applySymmetryToObject')
result = vol(volume.sizeX(),volume.sizeY(),volume.sizeZ())
result.copyVolume(volume)
sizeX = volume.sizeX()
sizeY = volume.sizeY()
sizeZ = volume.sizeZ()
rot = vol(sizeX,sizeY,sizeZ)
wedgeSum = vol(sizeX,sizeY,sizeZ/2+1)
wedgeSum.setAll(0.0)
angleList = symmetryObject.getAngleList()
rotation = angleList.nextRotation()
while not rotation == [None,None,None]:
rotate(volume,rot,rotation[0],rotation[1],rotation[2])
result = result + rot
rotation = angleList.nextRotation()
result.shiftscale(0.0,1/float(angleList.numberRotations()))
return result
