def subcellular_input_map(cellName, networkParamName, synapseFilename, outputFilename, cellType=None, tBegin=None, tEnd=None):
'''
visualization of subcellular input distributions:
-per cell type and time window possible
-output: per cell type landmark files, list of distances
'''
neuronParameters = scp.build_parameters(cellName)
networkParameters = scp.build_parameters(networkParamName)
scp.load_NMODL_parameters(neuronParameters)
scp.load_NMODL_parameters(networkParameters)
cellParam = neuronParameters.neuron
print 'Loading cell morphology...'
parser = scp.CellParser(cellParam.filename)
parser.spatialgraph_to_cell()
cell = parser.cell
synLocations = []
synDistances = []
if tBegin is None and tEnd is None:
evokedNW = scp.NetworkMapper(cell, networkParameters.network)
evokedNW._assign_anatomical_synapses()
synTypes = cell.synapses.keys()
for synType in synTypes:
preCellType, preColumn = synType.split('_')
if cellType is not None and preCellType != cellType:
continue
print 'Computing synapse distances for cell type %s' % synType
synDistances.extend(sca.compute_syn_distances(cell, synType))
for syn in cell.synapses[synType]:
secID = syn.secID
ptID = syn.ptID
x = syn.x
synLoc3D = cell.sections[secID].pts[ptID]
synLocations.append(synLoc3D)
else:
synInfo = scp.read_synapse_activation_file(synapseFilename)
synTypes = synInfo.keys()
for synType in synTypes:
preCellType, preColumn = synType.split('_')
if cellType is not None and preCellType != cellType:
continue
print 'Loading synapse distances and activation times for cell type %s' % synType
for syn in synInfo[synType]:
synID, secID, ptID, synTimes, somaDist = syn
activeSyn = False
for t in synTimes:
if tBegin <= t <= tEnd:
activeSyn = True
break
if not activeSyn:
continue
synLoc3D = cell.sections[secID].pts[ptID]
synLocations.append(synLoc3D)
synDistances.append(somaDist)
scp.write_landmark_file(outputFilename, synLocations)
write_synapse_distances(outputFilename, synDistances)
def create_network_parameter(templateParamName, cellNumberFileName,
synFileName, conFileName, outFileName):
print '*************'
print 'creating network parameter file from template %s' % templateParamName
print '*************'
templateParam = scp.build_parameters(templateParamName)
cellTypeColumnNumbers = load_cell_number_file(cellNumberFileName)
nwParam = scp.NTParameterSet({
'info':
templateParam.info,
'NMODL_mechanisms':
templateParam.NMODL_mechanisms
})
# nwParam.info = templateParam.info
# nwParam.NMODL_mechanisms = templateParam.NMODL_mechanisms
nwParam.network = {}
for cellType in cellTypeColumnNumbers.keys():
cellTypeParameters = templateParam.network[cellType]
for column in cellTypeColumnNumbers[cellType].keys():
numberOfCells = cellTypeColumnNumbers[cellType][column]
if numberOfCells == 0:
continue
cellTypeName = cellType + '_' + column
nwParam.network[cellTypeName] = cellTypeParameters.tree_copy()
nwParam.network[cellTypeName].cellNr = numberOfCells
nwParam.network[
cellTypeName].synapses.distributionFile = synFileName
nwParam.network[cellTypeName].synapses.connectionFile = conFileName
nwParam.save(outFileName)
def synapse_distances_2D(pname):
parameters = scp.build_parameters(pname)
cellParam = parameters.network.post
preParam = parameters.network.pre
parser = scp.CellParser(cellParam.filename)
parser.spatialgraph_to_cell()
cell = parser.cell
for preType in preParam.keys():
synapseFName = preParam[preType].synapses.realization
synDist = scp.read_synapse_realization(synapseFName)
mapper = scp.SynapseMapper(cell, synDist)
mapper.map_synapse_realization()
for synType in cell.synapses.keys():
dist = compute_syn_distances_2Dprojected(cell, synType)
name = parameters.info.outputname
name += '_'
name += synType
name += '_syn_distances_2Dprojected.csv'
with open(name, 'w') as distFile:
header = 'Distance to soma (micron)\n'
distFile.write(header)
for d in dist:
distFile.write(str(d) + '\n')
def remove_population(folderName, contains, suffix, population):
fnames = []
get_files_in_directory(folderName, fnames, contains, suffix)
for tmpName in fnames:
print '*************'
print 'Removing population %s from file %s' % (population, tmpName)
print '*************'
nwParamCluster = scp.build_parameters(tmpName)
nwParamCluster.network.pop(population)
insertIndex = tmpName.find('_UpState')
insertIndex += 8
clusterOutFileName = tmpName[:insertIndex]
clusterOutFileName += '_'
clusterOutFileName += population
clusterOutFileName += '_inact'
clusterOutFileName += tmpName[insertIndex:]
nwParamCluster.save(clusterOutFileName)
def create_network_parameter(templateParamName, cellNumberFileName,
synFileName, conFileName, whisker, outFileName):
print '*************'
print 'creating network parameter file from template %s' % templateParamName
print '*************'
templateParam = scp.build_parameters(templateParamName)
cellTypeColumnNumbers = load_cell_number_file(cellNumberFileName)
nwParam = scp.NTParameterSet({
'info':
templateParam.info,
'NMODL_mechanisms':
templateParam.NMODL_mechanisms
})
nwParam.network = {}
for cellType in templateParam.network.keys():
cellTypeParameters = templateParam.network[cellType]
for column in cellTypeColumnNumbers[cellType].keys():
numberOfCells = cellTypeColumnNumbers[cellType][column]
if numberOfCells == 0:
continue
cellTypeName = cellType + '_' + column
nwParam.network[cellTypeName] = cellTypeParameters.tree_copy()
PSTH = whisker_evoked_PSTH(column, whisker, cellType)
if PSTH is not None:
interval = nwParam.network[cellTypeName].pop('interval')
nwParam.network[cellTypeName].celltype = {
'spiketrain': {
'interval': interval
}
}
nwParam.network[cellTypeName].celltype['pointcell'] = PSTH
nwParam.network[cellTypeName].celltype['pointcell'][
'offset'] = deflectionOffset
nwParam.network[cellTypeName].cellNr = numberOfCells
nwParam.network[
cellTypeName].synapses.distributionFile = synFileName
nwParam.network[cellTypeName].synapses.connectionFile = conFileName
nwParam.save(outFileName)
L6ctEvokedName = evokedPrefix + 'L6ct_3x3_PSTH_UpState.param' VPMEvokedName = evokedPrefix + 'VPM_3x3_PSTH.param' L1EvokedName = evokedPrefix + 'L1_3x3_PSTH_UpState.param' L23TransEvokedName = evokedPrefix + 'L23Trans_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' L45PeakEvokedName = evokedPrefix + 'L45Peak_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' L45SymEvokedName = evokedPrefix + 'L45Sym_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' L56TransEvokedName = evokedPrefix + 'L56Trans_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal1EvokedName = evokedPrefix + 'SymLocal1_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal2EvokedName = evokedPrefix + 'SymLocal2_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal3EvokedName = evokedPrefix + 'SymLocal3_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal4EvokedName = evokedPrefix + 'SymLocal4_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal5EvokedName = evokedPrefix + 'SymLocal5_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' SymLocal6EvokedName = evokedPrefix + 'SymLocal6_PSTH_active_timing_normalized_PW_1.0_SuW_0.5.param' L2EvokedParam = scp.build_parameters(L2EvokedName) L34EvokedParam = scp.build_parameters(L34EvokedName) L4pyEvokedParam = scp.build_parameters(L4pyEvokedName) L4spEvokedParam = scp.build_parameters(L4spEvokedName) L4ssEvokedParam = scp.build_parameters(L4ssEvokedName) L5stEvokedParam = scp.build_parameters(L5stEvokedName) L5ttEvokedParam = scp.build_parameters(L5ttEvokedName) L6ccEvokedParam = scp.build_parameters(L6ccEvokedName) L6ccinvEvokedParam = scp.build_parameters(L6ccinvEvokedName) L6ctEvokedParam = scp.build_parameters(L6ctEvokedName) VPMEvokedParam = scp.build_parameters(VPMEvokedName) L1EvokedParam = scp.build_parameters(L1EvokedName) L23TransEvokedParam = scp.build_parameters(L23TransEvokedName) L45PeakEvokedParam = scp.build_parameters(L45PeakEvokedName) L45SymEvokedParam = scp.build_parameters(L45SymEvokedName) L56TransEvokedParam = scp.build_parameters(L56TransEvokedName)
def create_network_parameter(templateParamName, cellNumberFileName,
synFileName, conFileName, whisker, outFileName):
print '*************'
print 'creating network parameter file from template %s' % templateParamName
print '*************'
templateParam = scp.build_parameters(templateParamName)
cellTypeColumnNumbers = load_cell_number_file(cellNumberFileName)
nwParam = scp.NTParameterSet({
'info':
templateParam.info,
'NMODL_mechanisms':
templateParam.NMODL_mechanisms
})
# nwParam.info = templateParam.info
# nwParam.NMODL_mechanisms = templateParam.NMODL_mechanisms
nwParam.network = {}
if clusterParameters:
clusterBasePath = '/gpfs01/bethge/home/regger'
nwParamCluster = scp.NTParameterSet({'info': templateParam.info})
nwParamCluster.NMODL_mechanisms = templateParam.NMODL_mechanisms.tree_copy(
)
nwParamCluster.network = {}
synFileNameIndex = synFileName.find('L5tt')
synFileNameCluster = clusterBasePath + '/data/' + synFileName[
synFileNameIndex:]
conFileNameCluster = synFileNameCluster[:-4] + '.con'
for mech in nwParamCluster.NMODL_mechanisms:
mechPath = nwParamCluster.NMODL_mechanisms[mech]
if '/nas1/Data_regger' in mechPath:
mechPathIndex = mechPath.find('L5tt')
newMechPath = clusterBasePath + '/data/' + mechPath[
mechPathIndex:]
if '/home/regger' in mechPath:
newMechPath = clusterBasePath + mechPath[12:]
nwParamCluster.NMODL_mechanisms[mech] = newMechPath
# for cellType in cellTypeColumnNumbers.keys():
for cellType in templateParam.network.keys():
cellTypeParameters = templateParam.network[cellType]
for column in cellTypeColumnNumbers[cellType].keys():
numberOfCells = cellTypeColumnNumbers[cellType][column]
if numberOfCells == 0:
continue
cellTypeName = cellType + '_' + column
nwParam.network[cellTypeName] = cellTypeParameters.tree_copy()
if clusterParameters:
nwParamCluster.network[
cellTypeName] = cellTypeParameters.tree_copy()
PSTH = whisker_evoked_PSTH(column, whisker, cellType)
if PSTH is not None:
interval = nwParam.network[cellTypeName].pop('interval')
nwParam.network[cellTypeName].celltype = {
'spiketrain': {
'interval': interval
}
}
nwParam.network[cellTypeName].celltype['pointcell'] = PSTH
nwParam.network[cellTypeName].celltype['pointcell'][
'offset'] = deflectionOffset
if clusterParameters:
interval = nwParamCluster.network[cellTypeName].pop(
'interval')
nwParamCluster.network[cellTypeName].celltype = {
'spiketrain': {
'interval': interval
}
}
nwParamCluster.network[cellTypeName].celltype[
'pointcell'] = PSTH
nwParamCluster.network[cellTypeName].celltype['pointcell'][
'offset'] = deflectionOffset
nwParam.network[cellTypeName].cellNr = numberOfCells
nwParam.network[
cellTypeName].synapses.distributionFile = synFileName
nwParam.network[cellTypeName].synapses.connectionFile = conFileName
if clusterParameters:
nwParamCluster.network[cellTypeName].cellNr = numberOfCells
nwParamCluster.network[
cellTypeName].synapses.distributionFile = synFileNameCluster
nwParamCluster.network[
cellTypeName].synapses.connectionFile = conFileNameCluster
nwParam.save(outFileName)
clusterOutFileName = outFileName[:-6] + '_cluster.param'
if clusterParameters:
nwParamCluster.save(clusterOutFileName)
def assign_3D_soma_locations(cellName, networkParamName, somaDistributionName,
VPMFolderName, connectedSomataName):
'''
randomly assign 3D locations of corresponding cell type/column
Assumes there are >= somata than connected somata!!!
(i.e. best to use soma distribution from corresponding NeuroNet output)
'''
neuronParameters = scp.build_parameters(cellName)
networkParameters = scp.build_parameters(networkParamName)
scp.load_NMODL_parameters(neuronParameters)
scp.load_NMODL_parameters(networkParameters)
cellParam = neuronParameters.neuron
somaDistribution = load_soma_distribution(somaDistributionName)
somaDistribution['VPM'] = load_soma_distribution_VPM(VPMFolderName)
# connectedSomata = load_connected_somata(connectedSomataName)
#
# for cellType in connectedSomata.keys():
# somaLocations3D = []
# for column in connectedSomata[cellType].keys():
# numberOfConnectedCells = len(connectedSomata[cellType][column])
# numberOfTotalCells = len(somaDistribution[cellType][column])
# somaLocationIDs = np.random.permutation(numberOfTotalCells)
# for i in range(numberOfConnectedCells):
# ID = somaLocationIDs[i]
# loc = somaDistribution[cellType][column][ID]
# somaLocations3D.append(loc)
# cellTypeName = connectedSomataName[:-4]
# cellTypeName += '_'
# cellTypeName += cellType
# scp.write_landmark_file(cellTypeName, somaLocations3D)
print 'Loading cell morphology...'
parser = scp.CellParser(cellParam.filename)
parser.spatialgraph_to_cell()
cell = parser.cell
evokedNW = scp.NetworkMapper(cell, networkParameters.network)
evokedNW._assign_anatomical_synapses()
evokedNW._create_presyn_cells()
evokedNW._map_complete_anatomical_realization()
somaLocationsPerCellType3D = {}
somaLocationsPerSynType3D = {}
for synType in evokedNW.cells.keys():
cellType, column = synType.split('_')
numberOfConnectedCells = len(evokedNW.cells[synType])
numberOfTotalCells = len(somaDistribution[cellType][column])
somaLocationIDs = np.random.permutation(numberOfTotalCells)
for i in range(numberOfConnectedCells):
ID = somaLocationIDs[i]
loc = somaDistribution[cellType][column][ID]
try:
somaLocationsPerCellType3D[cellType].append(loc)
except KeyError:
somaLocationsPerCellType3D[cellType] = [loc]
try:
somaLocationsPerSynType3D[synType].append(loc)
except KeyError:
somaLocationsPerSynType3D[synType] = [loc]
for cellType in somaLocationsPerCellType3D.keys():
cellTypeName = connectedSomataName[:-4]
cellTypeName += '_'
cellTypeName += cellType
scp.write_landmark_file(cellTypeName,
somaLocationsPerCellType3D[cellType])
write_cell_location_realization(somaLocationsPerSynType3D,
connectedSomataName)
def evoked_activity(simName, cellName, evokedINHParamName,
evokedGenericParamName, evokedSynDistributionFile,
evokedInhSynDistributionFile, nrOfSynapses, synapseTiming):
'''
pre-stimulus ongoing activity
and evoked activity
'''
neuronParameters = scp.build_parameters(cellName)
evokedUpNWParameters = scp.build_parameters(evokedINHParamName)
evokedUpGenericParameters = scp.build_parameters(evokedGenericParamName)
scp.load_NMODL_parameters(neuronParameters)
scp.load_NMODL_parameters(evokedUpNWParameters)
scp.load_NMODL_parameters(evokedUpGenericParameters)
cellParam = neuronParameters.neuron
paramEvokedUp = evokedUpNWParameters.network
paramEvokedUpGeneric = evokedUpGenericParameters.network
cell = scp.create_cell(cellParam)
evokedSynDistribution = np.loadtxt(evokedSynDistributionFile,
skiprows=1,
unpack=True,
delimiter=',')
cellPointsLUT = cell_points_by_distance_LUT(cell, evokedSynDistribution,
'EXC')
evokedInhSynDistribution = np.loadtxt(evokedInhSynDistributionFile,
skiprows=1,
unpack=True,
delimiter=',')
cellPointsLUT_INH = cell_points_by_distance_LUT(cell,
evokedInhSynDistribution,
'INH')
offset = synapseTiming[0]
mode = synapseTiming[1]
median = synapseTiming[2]
mu = np.log(median)
sigma = np.sqrt(mu - np.log(mode))
synTimingLognormal = (offset, mu, sigma)
uniqueID = str(os.getpid())
dirName = 'results/'
dirName += simName
if not simName.endswith('/'):
dirName += '/'
dirName += time.strftime('%Y%m%d-%H%M')
dirName += '_'
dirName += uniqueID
if not os.path.exists(dirName):
os.makedirs(dirName)
vTraces = []
tTraces = []
recordingSiteFiles = neuronParameters.sim.recordingSites
recSiteManagers = []
for recFile in recordingSiteFiles:
recSiteManagers.append(sca.RecordingSiteManager(recFile, cell))
if 'PW' in simName:
nrOfInhSynapses = 375 # Avg PW deflection
elif 'SW' in simName:
nrOfInhSynapses = 209 # Avg SW deflection
elif 'E2' in simName:
nrOfInhSynapses = 255 # Avg E2 deflection
nSweeps = 200
tOffset = 100.0 # avoid numerical transients
tStim = 245.0
tStop = 270.0
neuronParameters.sim.tStop = tStop
dt = neuronParameters.sim.dt
offsetBin = int(tOffset / dt + 0.5)
nRun = 0
while nRun < nSweeps:
synParametersEvoked = paramEvokedUp
startTime = time.time()
evokedNW = scp.NetworkMapper(cell, synParametersEvoked,
neuronParameters.sim)
evokedNW.create_saved_network2()
place_synapses_by_distance(evokedSynDistribution, cell, cellPointsLUT,
nrOfSynapses, 'Generic')
activate_distance_dependent_synapses(cell, tStim, synTimingLognormal,
'Generic', paramEvokedUpGeneric)
place_synapses_by_distance(evokedInhSynDistribution, cell,
cellPointsLUT_INH, nrOfInhSynapses,
'GenericINH')
activate_distance_dependent_synapses(cell, tStim, None, 'GenericINH',
paramEvokedUpGeneric)
stopTime = time.time()
setupdt = stopTime - startTime
print 'Network setup time: %.2f s' % setupdt
synTypes = cell.synapses.keys()
synTypes.sort()
print 'Testing evoked response properties run %d of %d' % (nRun + 1,
nSweeps)
tVec = h.Vector()
tVec.record(h._ref_t)
startTime = time.time()
scp.init_neuron_run(neuronParameters.sim, vardt=False)
stopTime = time.time()
simdt = stopTime - startTime
print 'NEURON runtime: %.2f s' % simdt
vmSoma = np.array(cell.soma.recVList[0])
t = np.array(tVec)
vTraces.append(np.array(vmSoma[offsetBin:])), tTraces.append(
np.array(t[offsetBin:]))
for RSManager in recSiteManagers:
RSManager.update_recordings()
print 'writing simulation results'
fname = 'simulation'
fname += '_run%04d' % nRun
synName = dirName + '/' + fname + '_synapses.csv'
print 'computing active synapse properties'
sca.compute_synapse_distances_times(synName, cell, t, synTypes)
nRun += 1
cell.re_init_cell()
cell.synapses.pop('Generic')
cell.synapses.pop('GenericINH')
evokedNW.re_init_network()
print '-------------------------------'
vTraces = np.array(vTraces)
dendTraces = []
scp.write_all_traces(dirName + '/' + uniqueID + '_vm_all_traces.csv',
t[offsetBin:], vTraces)
for RSManager in recSiteManagers:
for recSite in RSManager.recordingSites:
tmpTraces = []
for vTrace in recSite.vRecordings:
tmpTraces.append(vTrace[offsetBin:])
recSiteName = dirName + '/' + uniqueID + '_' + recSite.label + '_vm_dend_traces.csv'
scp.write_all_traces(recSiteName, t[offsetBin:], tmpTraces)
dendTraces.append(tmpTraces)
dendTraces = np.array(dendTraces)
print 'writing simulation parameter files'
neuronParameters.save(dirName + '/' + uniqueID + '_neuron_model.param')
evokedUpNWParameters.save(dirName + '/' + uniqueID +
'_network_model.param')
def evoked_activity(simName, cellName, evokedUpParamName):
'''
pre-stimulus ongoing activity
and evoked activity
'''
neuronParameters = scp.build_parameters(cellName)
evokedUpNWParameters = scp.build_parameters(evokedUpParamName)
scp.load_NMODL_parameters(neuronParameters)
scp.load_NMODL_parameters(evokedUpNWParameters)
cellParam = neuronParameters.neuron
paramEvokedUp = evokedUpNWParameters.network
cell = scp.create_cell(cellParam)
uniqueID = str(os.getpid())
dirName = 'results/'
dirName += simName
if not simName.endswith('/'):
dirName += '/'
dirName += time.strftime('%Y%m%d-%H%M')
dirName += '_'
dirName += uniqueID
if not os.path.exists(dirName):
os.makedirs(dirName)
vTraces = []
tTraces = []
recordingSiteFiles = neuronParameters.sim.recordingSites
recSiteManagers = []
for recFile in recordingSiteFiles:
recSiteManagers.append(sca.RecordingSiteManager(recFile, cell))
nSweeps = 200
tOffset = 100.0 # avoid numerical transients
tStim = 245.0
tStop = 270.0
neuronParameters.sim.tStop = tStop
dt = neuronParameters.sim.dt
offsetBin = int(tOffset / dt + 0.5)
nRun = 0
while nRun < nSweeps:
synParametersEvoked = paramEvokedUp
startTime = time.time()
evokedNW = scp.NetworkMapper(cell, synParametersEvoked,
neuronParameters.sim)
evokedNW.create_saved_network2()
stopTime = time.time()
setupdt = stopTime - startTime
print 'Network setup time: %.2f s' % setupdt
synTypes = cell.synapses.keys()
synTypes.sort()
print 'Testing evoked response properties run %d of %d' % (nRun + 1,
nSweeps)
tVec = h.Vector()
tVec.record(h._ref_t)
startTime = time.time()
scp.init_neuron_run(neuronParameters.sim, vardt=False)
stopTime = time.time()
simdt = stopTime - startTime
print 'NEURON runtime: %.2f s' % simdt
vmSoma = np.array(cell.soma.recVList[0])
t = np.array(tVec)
vTraces.append(np.array(vmSoma[offsetBin:])), tTraces.append(
np.array(t[offsetBin:]))
for RSManager in recSiteManagers:
RSManager.update_recordings()
print 'writing simulation results'
fname = 'simulation'
fname += '_run%04d' % nRun
synName = dirName + '/' + fname + '_synapses.csv'
print 'computing active synapse properties'
sca.compute_synapse_distances_times(synName, cell, t, synTypes)
preSynCellsName = dirName + '/' + fname + '_presynaptic_cells.csv'
scp.write_presynaptic_spike_times(preSynCellsName, evokedNW.cells)
nRun += 1
cell.re_init_cell()
evokedNW.re_init_network()
print '-------------------------------'
vTraces = np.array(vTraces)
scp.write_all_traces(dirName + '/' + uniqueID + '_vm_all_traces.csv',
t[offsetBin:], vTraces)
for RSManager in recSiteManagers:
for recSite in RSManager.recordingSites:
tmpTraces = []
for vTrace in recSite.vRecordings:
tmpTraces.append(vTrace[offsetBin:])
recSiteName = dirName + '/' + uniqueID + '_' + recSite.label + '_vm_dend_traces.csv'
scp.write_all_traces(recSiteName, t[offsetBin:], tmpTraces)
print 'writing simulation parameter files'
neuronParameters.save(dirName + '/' + uniqueID + '_neuron_model.param')
evokedUpNWParameters.save(dirName + '/' + uniqueID +
'_network_model.param')
def single_neuron_embedding_to_catmaid(cellName, networkParamName,
somaDistributionName,
connectedNeuronsName, outputName):
'''
randomly assign 3D locations of corresponding cell type/column
Assumes there are >= somata than connected somata!!!
(i.e. best to use soma distribution from corresponding NeuroNet output)
'''
neuronParameters = scp.build_parameters(cellName)
networkParameters = scp.build_parameters(networkParamName)
scp.load_NMODL_parameters(neuronParameters)
scp.load_NMODL_parameters(networkParameters)
cellParam = neuronParameters.neuron
somaDistribution = load_cell_location_realization(somaDistributionName)
synapseConnectivity = load_connected_neuron_per_synapse(
connectedNeuronsName)
print 'Loading cell morphology...'
parser = scp.CellParser(cellParam.filename)
parser.spatialgraph_to_cell()
cell = parser.cell
evokedNW = scp.NetworkMapper(cell, networkParameters.network)
evokedNW._assign_anatomical_synapses()
evokedNW._create_presyn_cells()
evokedNW._map_complete_anatomical_realization()
# create continuous morphology IDs ('DUMMYIDXXXXX')
IDCounter = 0
cellTypeColumns = somaDistribution.keys()
for cellTypeColumn in cellTypeColumns:
cellType = cellTypeColumn.split('_')[0]
column = cellTypeColumn.split('_')[1]
if len(somaDistribution[cellTypeColumn]) != len(
evokedNW.cells[cellTypeColumn]):
errstr = 'Number of cells in Network not equal to number of somata'
raise RuntimeError(errstr)
for i in range(len(somaDistribution[cellTypeColumn])):
somaLoc = somaDistribution[cellTypeColumn][i]
pointCell = evokedNW.cells[cellTypeColumn][i]
morphologyID = 'DUMMYID%05d' % IDCounter
pointCell.cellTypeStr = cellType
pointCell.columnStr = column
pointCell.morphologyID = morphologyID
pointCell.somaLoc3D = somaLoc
IDCounter += 1
for i in range(len(synapseConnectivity[cellTypeColumn])):
syn = cell.synapses[cellTypeColumn][i]
preCellID = synapseConnectivity[cellTypeColumn][i]
preCell = evokedNW.cells[cellTypeColumn][preCellID]
syn.preCell = preCell
create_catmaid_connection(cell, syn)
# write cell file in JSON format
morphologyName = outputName
morphologyName += '_morphologies.json'
write_catmaid_morphology_file(morphologyName, evokedNW.cells)
# write synapse file in CortexInSilico/Catmaid format
synapseName = outputName
synapseName += '_synapses.csv'
write_catmaid_synapse_file(synapseName, cell.synapses)
