def get_reaction(self):
rxn = MasterMix(self.buffers[self.transfer_type])
rxn.extra_percent = 20
rxn.extra_min_volume = '1 µL'
if isinstance(self.samples, int):
rxn.num_reactions = self.samples
else:
rxn['DNA'].name = ','.join(self.samples)
rxn.num_reactions = len(self.samples)
if x := self.num_spots:
rxn.num_reactions = x
def test_master_mix_scale(params):
mm = MasterMix()
mm.num_reactions = params['n']
mm.solvent = 'w'
mm.volume = params['vol_rxn']
mm['x'].volume = params['vol_x']
mm['w'].master_mix = True
mm['x'].master_mix = True
if 'fraction' in params:
mm.extra_fraction = params['fraction']
assert mm.extra_percent == pytest.approx(params['fraction'] * 100)
if 'percent' in params:
mm.extra_percent = params['percent']
assert mm.extra_fraction == pytest.approx(params['percent'] / 100)
if 'reactions' in params:
mm.extra_reactions = params['reactions']
if 'min_vol' in params:
mm.extra_min_volume = params['min_vol']
assert mm.get_scale() == pytest.approx(params['expected'])