def fetch_assembly_meta_xml(accession):
"""
Fetch assembly metadata xml from ENA.
"""
url = "%s/xml/%s" % (ENA_API, accession)
xml = tofetch.fetch_url(url)
return xml
def fetch_goat_data(taxon_id):
"""Fetch taxon metadata from GoaT."""
LOGGER.info("Fetching taxon metadata")
url = "%s/record?recordId=taxon_id-%s&result=taxon" % (GOAT_API, taxon_id)
result = tofetch.fetch_url(url)
if result is None:
LOGGER.error("Unable to fetch taxon metadata for '%s' from GoaT",
taxon_id)
sys.exit(1)
data = ujson.loads(result)
return data["records"][0]["record"]
def fetch_accession(bioproject):
"""Fetch a GCA accession for a bioproject."""
LOGGER.info("Fetching GCA accession for bioproject %s" % bioproject)
url = "%s/search?result=assembly&query=study_accession%%3D%%22%s%%22&fields=accession%%2Cversion&format=tsv" % (
ENA_API,
bioproject,
)
result = tofetch.fetch_url(url)
accession = None
if result and result is not None:
line = result.split("\n")[1]
if line and "\t" in line:
accession, version = line.split("\t")
accession += ".%s" % version
return accession
def fetch_bioproject_children(bioproject,
*,
projects=None,
children=None,
new_projects=None):
"""
Fetch children of a bioproject.
"""
if projects is None:
projects = []
if new_projects is None:
new_projects = {}
if children is None:
children = []
url = "%s/search?result=study&query=parent_study%%3D%%22%s%%22&format=tsv" % (
ENA_API,
bioproject,
)
result = tofetch.fetch_url(url)
if result and result is not None:
for line in result.split("\n")[1:]:
if line and "\t" in line:
child_accession, description = line.split("\t")
if child_accession in projects:
continue
if "genome assembly" in description:
if "alternate haplotype" not in description:
children.append(child_accession)
new_projects.update({child_accession: bioproject})
else:
sleep(0.5)
LOGGER.info(
"Fetching nested accessions under bioproject %s" %
child_accession)
fetch_bioproject_children(
child_accession,
projects=projects,
children=children,
new_projects=new_projects,
)
return children, new_projects
def fetch_busco_lineages(busco_sets, buscodir):
"""Fetch busco lineages."""
if not busco_sets:
return
lineages_to_fetch = []
for lineage in busco_sets:
busco_lineage = "%s/lineages/%s" % (buscodir, lineage)
if not os.path.isdir(busco_lineage):
lineages_to_fetch.append(lineage)
if not lineages_to_fetch:
return
lineage_urls = {}
LOGGER.info("Fetching BUSCO lineage directory listing")
listing = tofetch.fetch_url("%s/" % BUSCO_URL)
for entry in listing.split("\n"):
parts = re.split(r"[\"\s]+", entry)
if len(parts) == 8:
busco_set = re.sub(r"\..+$", "", parts[2])
lineage_urls.update({busco_set: "%s/%s" % (BUSCO_URL, parts[2])})
for lineage in lineages_to_fetch:
LOGGER.info("Fetching BUSCO lineage %s" % lineage)
tofetch.fetch_tar(lineage_urls[lineage], buscodir)
def fetch_read_info(accession, per_platform):
"""Fetch read info for an accession."""
portal = "https://www.ebi.ac.uk/ena/portal/api"
url = (
"%s/filereport?accession=%s&result=read_run&fields=run_accession,fastq_bytes,base_count,library_strategy,library_selection,library_layout,instrument_platform,experiment_title,fastq_ftp"
% (portal, accession))
data = tofetch.fetch_url(url)
if data is None:
return
header = None
for line in data.split("\n"):
if not line or line == "":
continue
if header is None:
header = line.split("\t")
continue
fields = line.split("\t")
values = {}
platform = "OTHER"
for i in range(0, len(header)):
value = fields[i]
if header[
i] == "instrument_platform" and platform != "ILLUMINA_XTEN":
platform = fields[i]
if header[i] == "experiment_title":
if value == "HiSeq X Ten paired end sequencing":
platform = "ILLUMINA_XTEN"
values.update({header[i]: value})
if "base_count" in values:
values["base_count"] = int(values["base_count"])
else:
values["base_count"] = 0
try:
per_platform[platform].append(values)
except KeyError:
per_platform["OTHER"].append(values)
