def test_gene_expression(self):
# Set up the standard TXTL tubes
tube1 = txtl.extract('BL21_DE3')
tube2 = txtl.buffer('stdbuffer')
# Now set up a tube that will contain our DNA
tube3 = txtl.newtube('geneexpr')
# Define a DNA strand using strings (ala MATLAB)
gene1 = txtl.assemble_dna('ptet(50)', 'BCD2(20)', 'tetR(1200)')
txtl.add_dna(tube3, gene1, 1, 'plasmid')
# Create individual DNA components based on standard types
ptet = txtl.RepressedPromoter('ptet', 'tetR', dimer=True)
bcd2 = txtl.ConstitutiveRBS('BCD2', Ribosome_Binding_F=10)
degfp = txtl.ProteinCDS('deGFP', maturation_time=30 * txtl.minutes)
lva = txtl.DegradationTag('lva', 'clpXP')
# Assemble a gene using objects instead of strings
gene2 = txtl.assemble_dna(ptet, bcd2, degfp, lva)
txtl.add_dna(tube3, gene2, 1, 'plasmid')
# Mix the contents of the individual tubes
well1 = txtl.combine_tubes([tube1, tube2, tube3])
# Create an SBML file containing the model
txtl.write_sbml(well1, 'geneexpr.xml')
def test_assemble_objects(self):
mix = txtl.Mixture('testmix')
ptet = txtl.RepressedPromoter('ptet', 'tetR', dimer=True)
bcd2 = txtl.ConstitutiveRBS('BCD2', Ribosome_Binding_F=10)
degfp = txtl.ProteinCDS('deGFP', maturation_time=30 * txtl.minutes)
lva = txtl.DegradationTag('lva', 'clpXP')
gene = txtl.assemble_dna(ptet, bcd2, degfp, lva)
txtl.add_dna(mix, gene, 1, 'plasmid')
def test_override_parameters(self):
bcd2 = txtl.ConstitutiveRBS('BCD2',
parameters = {Ribosome_Binding_F:10})
def test_override_parameter_keyword(self):
# Override a single parameter
bcd2 = txtl.ConstitutiveRBS('BCD2', Ribosome_Binding_F=10)
degfp = txtl.ProteinCDS('deGFP', maturation_time=30*txtl.minutes)
tube3 = txtl.newtube('geneexpr')
# Define a DNA strand using strings (ala MATLAB)
gene1 = txtl.assemble_dna(prom='ptet(50)', utr5='BCD2(20)', cds='tetR(1200)')
txtl.add_dna(mixture=tube3, dna=gene1, conc=1, type='plasmid')
#
# Assemble a DNA strand using objects (semi-pythonic)
#
# Note: these constructs would normally live inside of a model
# library, but this shows how to extend functionality by creating
# constructs inline.
# Create individual DNA components based on standard types
ptet = txtl.RepressedPromoter('ptet', 'tetR', dimer=True)
bcd2 = txtl.ConstitutiveRBS('BCD2', Ribosome_Binding_F=10)
degfp = txtl.ProteinCDS('deGFP', maturation_time=30 * txtl.minutes)
lva = txtl.DegradationTag('lva', 'clpXP')
# Assemble a gene using objects instead of strings
gene2 = txtl.assemble_dna(ptet, bcd2, degfp, lva)
txtl.add_dna(tube3, gene2, 1, 'plasmid')
# Mix the contents of the individual tubes
well1 = txtl.combine_tubes([tube1, tube2, tube3])
# Run a simulation
#! TODO: implement
simdata = txtl.bioscrape.runsim(well1, 8 * txtl.hours)
# plot the result