def __init__(self, args, base_plotdir, skip_boring_states=''):
raise Exception('needs to be converted off root')
self.base_plotdir = base_plotdir
self.skip_boring_states = skip_boring_states
plot_types = ('transitions', 'emissions')
for ptype in plot_types:
plotdir = self.base_plotdir + '/' + ptype + '/plots'
utils.prep_dir(plotdir, wildlings='*.png')
if args.hmmdir != None:
filelist = glob.glob(args.hmmdir + '/*.yaml')
else:
filelist = utils.get_arg_list(args.infiles)
if len(filelist) == 0:
print 'ERROR zero files passed to modelplotter'
sys.exit()
for infname in filelist:
gene_name = os.path.basename(infname).replace('.yaml', '') # the sanitized name, actually
# # ----------------------------------------------------------------------------------------
# if utils.get_region(gene_name) == 'v' and 'IGHV4-39_star_' not in gene_name:
# continue
# # ----------------------------------------------------------------------------------------
with open(infname) as infile:
model = yaml.load(infile)
self.make_transition_plot(gene_name, model)
self.make_emission_plot(gene_name, model)
for ptype in plot_types:
check_call(['./bin/makeHtml', self.base_plotdir + '/' + ptype, '1', 'null', 'png'])
check_call(['./bin/permissify-www', self.base_plotdir])
def plot(self, plotdir, partition=None, infiles=None, annotations=None, only_csv=None):
print ' plotting partitions'
sys.stdout.flush()
start = time.time()
for subdir in self.subplotdirs:
utils.prep_dir(plotdir + '/' + subdir, wildlings=['*.csv', '*.svg'])
if partition is not None: # one partition
assert infiles is None
assert annotations is not None
csize_hists = {'best' : plotting.get_cluster_size_hist(partition)}
self.plot_within_vs_between_hists(partition, annotations, plotdir)
elif infiles is not None: # plot the mean of a partition from each file
subset_hists = []
for fname in infiles:
cp = ClusterPath()
cp.readfile(fname)
subset_hists.append(plotting.get_cluster_size_hist(cp.partitions[cp.i_best]))
csize_hists = {'best' : plotting.make_mean_hist(subset_hists)}
for ih in range(len(subset_hists)):
subset_hists[ih].write(plotdir + ('/subset-%d-cluster-sizes.csv' % ih))
else:
assert False
plotting.plot_cluster_size_hists(plotdir + '/overall/cluster-sizes.svg', csize_hists, title='', log='x')
if not only_csv:
for subdir in self.subplotdirs:
plotting.make_html(plotdir + '/' + subdir)
print '(%.1f sec)' % (time.time()-start)
def clean_plots(self, plotdir):
self.mfreqer.clean_plots(plotdir + '/mute-freqs')
utils.prep_dir(plotdir + '/overall', wildlings=('*.csv', '*.svg'))
for column in self.counts:
if column in self.columns_to_subset_by_gene:
thisplotdir = plotdir + '/' + column
utils.prep_dir(thisplotdir, wildlings=['*.csv', '*.svg'])
def plot(self, plotdir, only_csv=False):
utils.prep_dir(plotdir, wildling=None, multilings=['*.csv', '*.svg', '*.root'])
for column in self.values:
if self.only_correct_gene_fractions and column not in bool_columns:
continue
if column in bool_columns:
right = self.values[column]['right']
wrong = self.values[column]['wrong']
errs = fraction_uncertainty.err(right, right+wrong)
print ' %s\n correct up to allele: %4d / %-4d = %4.4f (-%.3f, +%.3f)' % (column, right, right+wrong, float(right) / (right + wrong), errs[0], errs[1])
hist = plotting.make_bool_hist(right, wrong, self.name + '-' + column)
plotting.draw_no_root(hist, plotname=column, plotdir=plotdir, write_csv=True, stats='0-bin', only_csv=only_csv)
else:
# TODO this is dumb... I should make the integer-valued ones histograms as well
hist = plotting.make_hist_from_dict_of_counts(self.values[column], 'int', self.name + '-' + column, normalize=True)
log = ''
if column.find('hamming_to_true_naive') >= 0: # TODO why doesn't this just use the config dicts in plotheaders or wherever?
hist.title = 'hamming distance'
else:
hist.title = 'inferred - true'
plotting.draw_no_root(hist, plotname=column, plotdir=plotdir, write_csv=True, log=log, only_csv=only_csv)
for column in self.hists:
plotting.draw_no_root(self.hists[column], plotname=column, plotdir=plotdir, write_csv=True, log=log, only_csv=only_csv)
if not only_csv:
plotting.make_html(plotdir)
def clean_plots(self, plotdir, subset_by_gene):
self.mfreqer.clean_plots(plotdir + '/mute-freqs')
utils.prep_dir(plotdir + '/overall') #, multilings=('*.csv', '*.svg'))
for column in self.counts:
if subset_by_gene and ('_del' in column or column == 'vd_insertion' or column == 'dj_insertion'): # option to subset deletion and (real) insertion plots by gene
thisplotdir = plotdir + '/' + column
utils.prep_dir(thisplotdir, wildlings=['*.csv', '*.svg'])
def prep_cmdfo(iclust, seqfos, queries_to_include, color_scale_vals,
title):
subworkdir = '%s/mds-%d' % (self.args.workdir, iclust)
utils.prep_dir(subworkdir)
tmpfname = '%s/seqs.fa' % subworkdir
with open(tmpfname, 'w') as tmpfile:
for sfo in seqfos:
csval = None
if sfo['name'] in color_scale_vals:
csval = color_scale_vals[sfo['name']]
tmpfile.write(
'>%s%s\n%s\n' %
(sfo['name'],
(' %d' % csval) if csval is not None else '',
sfo['seq']))
cmdstr = './bin/mds-run.py %s --aligned --plotdir %s --plotname %s --workdir %s --seed %d' % (
tmpfname, plotdir, get_fname(iclust), subworkdir,
self.args.seed)
if queries_to_include is not None:
cmdstr += ' --queries-to-include %s' % ':'.join(
queries_to_include)
if title is not None:
cmdstr += ' --title=%s' % title.replace(' ', '@')
return {
'cmd_str': cmdstr,
'workdir': subworkdir,
'outfname': '%s/%s.svg' % (plotdir, get_fname(iclust)),
'workfnames': [tmpfname]
}
def write(self, base_outdir, mean_freq_outfname):
if not self.finalized:
self.finalize()
outdir = base_outdir + '/mute-freqs'
utils.prep_dir(outdir, '*.csv')
for gene in self.counts:
counts, freqs, plotting_info = self.counts[gene], self.freqs[gene], self.plotting_info[gene]
sorted_positions = sorted(counts)
outfname = outdir + '/' + utils.sanitize_name(gene) + '.csv'
with opener('w')(outfname) as outfile:
nuke_header = []
for nuke in utils.nukes:
nuke_header.append(nuke)
nuke_header.append(nuke + '_lo_err')
nuke_header.append(nuke + '_hi_err')
writer = csv.DictWriter(outfile, ('position', 'mute_freq', 'lo_err', 'hi_err') + tuple(nuke_header))
writer.writeheader()
for position in sorted_positions:
row = {'position':position,
'mute_freq':counts[position]['freq'],
'lo_err':counts[position]['freq_lo_err'],
'hi_err':counts[position]['freq_hi_err']}
for nuke in utils.nukes:
row[nuke] = freqs[position][nuke]
row[nuke + '_lo_err'] = freqs[position][nuke + '_lo_err']
row[nuke + '_hi_err'] = freqs[position][nuke + '_hi_err']
writer.writerow(row)
assert 'REGION' in mean_freq_outfname
self.mean_rates['all'].write(mean_freq_outfname.replace('REGION', 'all')) # hackey hackey hackey replacement... *sigh*
for region in utils.regions:
self.mean_rates[region].write(mean_freq_outfname.replace('REGION', region))
def __init__(self, args, base_plotdir, skip_boring_states=''):
self.base_plotdir = base_plotdir
self.skip_boring_states = skip_boring_states
plot_types = ('transitions', 'emissions', 'pair-emissions')
for ptype in plot_types:
plotdir = self.base_plotdir + '/' + ptype + '/plots'
utils.prep_dir(plotdir, '*.png')
if args.hmmdir != None:
filelist = glob.glob(args.hmmdir + '/*.yaml')
else:
filelist = utils.get_arg_list(args.infiles)
if len(filelist) == 0:
print 'ERROR zero files passed to modelplotter'
sys.exit()
for infname in filelist:
gene_name = os.path.basename(infname).replace(
'.yaml', '') # the sanitized name, actually
# # ----------------------------------------------------------------------------------------
# if utils.get_region(gene_name) == 'v' and 'IGHV4-39_star_' not in gene_name:
# continue
# # ----------------------------------------------------------------------------------------
with open(infname) as infile:
model = yaml.load(infile)
self.make_transition_plot(gene_name, model)
self.make_emission_plot(gene_name, model)
# self.make_pair_emission_plot(gene_name, model)
for ptype in plot_types:
check_call([
'./bin/makeHtml', self.base_plotdir + '/' + ptype, '1', 'null',
'png'
])
check_call(['./bin/permissify-www', self.base_plotdir])
def write_vdjalign_input(self, base_infname, n_procs):
n_remaining = len(self.remaining_queries)
queries_per_proc = float(n_remaining) / n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
written_queries = set() # make sure we actually write each query TODO remove this when you work out where they're disappearing to
if n_procs == 1: # double check for rounding problems or whatnot
assert n_queries_per_proc == n_remaining
for iproc in range(n_procs):
workdir = self.subworkdir(iproc, n_procs)
if n_procs > 1:
utils.prep_dir(workdir)
with opener('w')(workdir + '/' + base_infname) as sub_infile:
iquery = 0
for query_name in self.remaining_queries: # NOTE this is wasteful to loop of all the remaining queries for each process... but maybe not that wasteful
if iquery >= n_remaining:
break
if iquery < iproc*n_queries_per_proc or iquery >= (iproc + 1)*n_queries_per_proc: # not for this process
iquery += 1
continue
sub_infile.write('>' + query_name + ' NUKES\n')
seq = self.input_info[query_name]['seq']
if query_name in self.info['indels']:
seq = self.info['indels'][query_name]['reversed_seq'] # use the query sequence with shm insertions and deletions reversed
sub_infile.write(seq + '\n')
written_queries.add(query_name)
iquery += 1
not_written = self.remaining_queries - written_queries
if len(not_written) > 0:
raise Exception('didn\'t write %s to %s' % (':'.join(not_written), self.args.workdir))
def plot(self, plotdir, subset_by_gene=False, cyst_positions=None, tryp_positions=None):
print ' plotting parameters'
start = time.time()
utils.prep_dir(plotdir + '/plots') #, multilings=('*.csv', '*.svg'))
for column in self.counts:
if column == 'all':
continue
values, gene_values = {}, {}
if len(self.counts[column]) == 0:
print 'ERROR no counts in %s' % column
assert False
for index, count in self.counts[column].iteritems():
gene = None
if subset_by_gene and ('_del' in column or column == 'vd_insertion' or column == 'dj_insertion'): # option to subset deletion and (real) insertion plots by gene
if '_del' in column:
region = column[0]
else:
region = column[1]
assert region in utils.regions
assert 'IGH' + region.upper() in index[1] # NOTE this is hackey, but it works find now and will fail obviously
gene = index[1] # if I ever change the correlations to be incompatible. so screw it
if gene not in gene_values:
gene_values[gene] = {}
column_val = index[0]
if gene is not None:
if column_val not in gene_values[gene]:
gene_values[gene][column_val] = 0.0
gene_values[gene][column_val] += count
if column_val not in values:
values[column_val] = 0.0
values[column_val] += count
try: # figure out whether this is an integer or string (only used outside this loop when we make the plots)
int(column_val)
var_type = 'int'
except:
var_type = 'string'
if subset_by_gene and ('_del' in column or column == 'vd_insertion' or column == 'dj_insertion'): # option to subset deletion and (real) insertion plots by gene
thisplotdir = plotdir + '/' + column
utils.prep_dir(thisplotdir + '/plots', multilings=['*.csv', '*.svg'])
for gene in gene_values:
plotname = utils.sanitize_name(gene) + '-' + column
hist = plotting.make_hist_from_dict_of_counts(gene_values[gene], var_type, plotname, sort=True)
plotting.draw(hist, var_type, plotname=plotname, plotdir=thisplotdir, errors=True, write_csv=True)
check_call(['./bin/makeHtml', thisplotdir, '3', 'null', 'svg'])
check_call(['./bin/permissify-www', thisplotdir]) # NOTE this should really permissify starting a few directories higher up
plotname = column
hist = plotting.make_hist_from_dict_of_counts(values, var_type, plotname, sort=True)
plotting.draw(hist, var_type, plotname=plotname, plotdir=plotdir, errors=True, write_csv=True)
self.mutefreqer.plot(plotdir, cyst_positions, tryp_positions) #, mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv') # REGION is replace by each region in the three output files
if has_root:
check_call(['./bin/makeHtml', plotdir, '3', 'null', 'svg'])
check_call(['./bin/permissify-www', plotdir]) # NOTE this should really permissify starting a few directories higher up
print ' parameter plot time: %.3f' % (time.time()-start)
def clean_plots(self, plotdir):
self.mfreqer.clean_plots(plotdir + "/mute-freqs")
utils.prep_dir(plotdir + "/overall") # , multilings=('*.csv', '*.svg'))
for column in self.counts:
if column in self.columns_to_subset_by_gene:
thisplotdir = plotdir + "/" + column
utils.prep_dir(thisplotdir, wildlings=["*.csv", "*.svg"])
def plot(self, plotdir):
utils.prep_dir(plotdir + '/plots', wildling=None, multilings=['*.csv', '*.svg', '*.root'])
for column in self.values:
if self.only_correct_gene_fractions and column not in bool_columns:
continue
if column in bool_columns:
right = self.values[column]['right']
wrong = self.values[column]['wrong']
errs = fraction_uncertainty.err(right, right+wrong)
print ' %s\n correct up to allele: %4d / %-4d = %4.4f (-%.3f, +%.3f)' % (column, right, right+wrong, float(right) / (right + wrong), errs[0], errs[1])
hist = plotting.make_bool_hist(right, wrong, self.name + '-' + column)
plotting.draw(hist, 'bool', plotname=column, plotdir=plotdir, write_csv=True)
else:
# TODO this is dumb... I should make the integer-valued ones histograms as well
hist = plotting.make_hist_from_dict_of_counts(self.values[column], 'int', self.name + '-' + column, normalize=True)
log = ''
if column.find('hamming_to_true_naive') >= 0:
hist.GetXaxis().SetTitle('hamming distance')
else:
hist.GetXaxis().SetTitle('inferred - true')
plotting.draw(hist, 'int', plotname=column, plotdir=plotdir, write_csv=True, log=log)
for column in self.hists:
hist = plotting.make_hist_from_my_hist_class(self.hists[column], column)
plotting.draw(hist, 'float', plotname=column, plotdir=plotdir, write_csv=True, log=log)
check_call(['./bin/makeHtml', plotdir, '3', 'null', 'svg'])
check_call(['./bin/permissify-www', plotdir]) # NOTE this should really permissify starting a few directories higher up
def plot(self, base_plotdir, only_csv=False):
if not self.finalized:
self.finalize(debug=debug)
plotdir = base_plotdir + '/allele-finding'
for old_gene_dir in glob.glob(plotdir + '/*'): # has to be a bit more hackey than elsewhere, since we have no way of knowing what genes might have had their own directories written last time we wrote to this dir
if not os.path.isdir(old_gene_dir):
raise Exception('not a directory: %s' % old_gene_dir)
utils.prep_dir(old_gene_dir, wildlings=('*.csv', '*.svg'))
os.rmdir(old_gene_dir)
utils.prep_dir(plotdir, wildlings=('*.csv', '*.svg'))
if only_csv: # not implemented
return
start = time.time()
for gene in self.plotvals:
if utils.get_region(gene) != 'v':
continue
for position in self.plotvals[gene]:
if position not in self.fitted_positions[gene]: # we can make plots for the positions we didn't fit, but there's a *lot* of them and they're slow
continue
# if 'allele-finding' not in self.TMPxyvals[gene][position] or self.TMPxyvals[gene][position]['allele-finding'] is None:
# continue
plotting.make_allele_finding_plot(plotdir + '/' + utils.sanitize_name(gene), gene, position, self.plotvals[gene][position])
print ' allele finding plot time: %.1f' % (time.time()-start)
def plot(self, plotdir, subset_by_gene=False, cyst_positions=None, tryp_positions=None):
print ' plotting parameters'
# start = time.time()
utils.prep_dir(plotdir + '/plots') #, multilings=('*.csv', '*.svg'))
for column in self.counts:
if column == 'all':
continue
values, gene_values = {}, {}
if len(self.counts[column]) == 0:
print 'ERROR no counts in %s' % column
assert False
for index, count in self.counts[column].iteritems():
gene = None
if subset_by_gene and ('_del' in column or column == 'vd_insertion' or column == 'dj_insertion'): # option to subset deletion and (real) insertion plots by gene
if '_del' in column:
region = column[0]
else:
region = column[1]
assert region in utils.regions
assert 'IGH' + region.upper() in index[1] # NOTE this is hackey, but it works find now and will fail obviously
gene = index[1] # if I ever change the correlations to be incompatible. so screw it
if gene not in gene_values:
gene_values[gene] = {}
column_val = index[0]
if gene is not None:
if column_val not in gene_values[gene]:
gene_values[gene][column_val] = 0.0
gene_values[gene][column_val] += count
if column_val not in values:
values[column_val] = 0.0
values[column_val] += count
try: # figure out whether this is an integer or string (only used outside this loop when we make the plots)
int(column_val)
var_type = 'int'
except:
var_type = 'string'
if subset_by_gene and ('_del' in column or column == 'vd_insertion' or column == 'dj_insertion'): # option to subset deletion and (real) insertion plots by gene
thisplotdir = plotdir + '/' + column
utils.prep_dir(thisplotdir + '/plots', multilings=['*.csv', '*.svg'])
for gene in gene_values:
plotname = utils.sanitize_name(gene) + '-' + column
hist = plotting.make_hist_from_dict_of_counts(gene_values[gene], var_type, plotname, sort=True)
plotting.draw(hist, var_type, plotname=plotname, plotdir=thisplotdir, errors=True, write_csv=True)
check_call(['./bin/makeHtml', thisplotdir, '3', 'null', 'svg'])
check_call(['./bin/permissify-www', thisplotdir]) # NOTE this should really permissify starting a few directories higher up
plotname = column
hist = plotting.make_hist_from_dict_of_counts(values, var_type, plotname, sort=True)
plotting.draw(hist, var_type, plotname=plotname, plotdir=plotdir, errors=True, write_csv=True)
self.mutefreqer.plot(plotdir, cyst_positions, tryp_positions) #, mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv') # REGION is replace by each region in the three output files
if has_root:
check_call(['./bin/makeHtml', plotdir, '3', 'null', 'svg'])
check_call(['./bin/permissify-www', plotdir]) # NOTE this should really permissify starting a few directories higher up
def __init__(self, args, seed, sublabel=None):
self.args = args
if sublabel == None:
self.workdir = self.args.workdir + '/recombinator'
self.outfname = self.args.outfname
else: # need a separate workdir for each subprocess
self.workdir = self.args.workdir + '/recombinator-' + sublabel
self.outfname = self.workdir + '/' + os.path.basename(self.args.outfname)
utils.prep_dir(self.workdir)
if not os.path.exists(self.args.parameter_dir):
raise Exception('parameter dir ' + self.args.parameter_dir + ' d.n.e')
# parameters that control recombination, erosion, and whatnot
self.index_keys = {} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.version_freq_table = {} # list of the probabilities with which each VDJ combo appears in data
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
# first read info that doesn't depend on which person we're looking at
self.glfo = utils.read_germline_set(self.args.datadir)
# then read stuff that's specific to each person
self.read_vdj_version_freqs(self.args.parameter_dir + '/' + utils.get_parameter_fname('all'))
self.allowed_genes = self.get_allowed_genes(self.args.parameter_dir) # only really used if <self.args.uniform_vj_choice_probs> is set, but it also checks the sensibility of <self.args.only_genes>
self.insertion_content_probs = None
self.read_insertion_content()
# read shm info NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with opener('r')(self.args.gtrfname) as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line['value']
treegen = treegenerator.TreeGenerator(args, self.args.parameter_dir, seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(seed, self.treefname)
with opener('r')(self.treefname) as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
if not self.args.no_clean:
os.remove(self.treefname)
if os.path.exists(self.outfname):
os.remove(self.outfname)
elif not os.path.exists(os.path.dirname(os.path.abspath(self.outfname))):
os.makedirs(os.path.dirname(os.path.abspath(self.outfname)))
def __init__(self, args, seed, sublabel=None):
self.args = args
if sublabel == None:
self.workdir = self.args.workdir + '/recombinator'
self.outfname = self.args.outfname
else: # need a separate workdir for each subprocess
self.workdir = self.args.workdir + '/recombinator-' + sublabel
self.outfname = self.workdir + '/' + os.path.basename(self.args.outfname)
utils.prep_dir(self.workdir)
if not self.args.simulate_partially_from_scratch:
parameter_dir = self.args.parameter_dir
else: # we start from scratch, except for the mute freq stuff
parameter_dir = self.args.scratch_mute_freq_dir
if parameter_dir is None or not os.path.exists(parameter_dir):
raise Exception('parameter dir ' + parameter_dir + ' d.n.e')
self.index_keys = {} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
self.glfo = glutils.read_glfo(self.args.initial_datadir, self.args.chain, only_genes=self.args.only_genes)
self.allowed_genes = self.get_allowed_genes(parameter_dir) # set of genes a) for which we read per-position mutation information and b) from which we choose when running partially from scratch
self.version_freq_table = self.read_vdj_version_freqs(parameter_dir) # list of the probabilities with which each VDJ combo (plus other rearrangement parameters) appears in data
self.insertion_content_probs = self.read_insertion_content(parameter_dir)
self.all_mute_freqs = {}
self.parameter_dir = parameter_dir # damnit, I guess I do need to save this in self
# read shm info NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with opener('r')(self.args.gtrfname) as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line['value']
treegen = treegenerator.TreeGenerator(args, parameter_dir, seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(seed, self.treefname)
with opener('r')(self.treefname) as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
os.remove(self.treefname)
if os.path.exists(self.outfname):
os.remove(self.outfname)
elif not os.path.exists(os.path.dirname(os.path.abspath(self.outfname))):
os.makedirs(os.path.dirname(os.path.abspath(self.outfname)))
def run_sklearn_mds(n_components, n_clusters, seqfos, seed, reco_info=None, region=None, aligned=False, n_init=4, max_iter=300, eps=1e-3, n_jobs=-1, plotdir=None, debug=False):
# NOTE set <n_components> to None to run plain kmeans, without mds TODO clean this up
start = time.time()
assert n_clusters is not None
if 'sklearn' not in sys.modules:
from sklearn import manifold # these are both slow af to import, even on local ssd
from sklearn.cluster import KMeans
if len(set(sfo['name'] for sfo in seqfos)) != len(seqfos):
raise Exception('duplicate sequence ids in <seqfos>')
if not aligned: # NOTE unlike the bios2mds version above, this modifies <seqfos>
if debug:
print 'align'
seqfos = utils.align_many_seqs(seqfos)
if debug:
print ' distances'
# translations = string.maketrans('ACGT-', '01234')
# def convert(seq):
# return [int(c) for c in seq.translate(translations)]
# converted_seqs = [convert(x['seq']) for x in seqfos]
# similarities = scipy.spatial.distance.pdist(converted_seqs, 'hamming')
# similarities = scipy.spatial.distance.squareform(similarities)
similarities = scipy.spatial.distance.squareform([utils.hamming_fraction(seqfos[i]['seq'], seqfos[j]['seq']) for i in range(len(seqfos)) for j in range(i + 1, len(seqfos))])
random_state = numpy.random.RandomState(seed=seed)
pos = None
if n_components is not None:
if debug:
print ' mds'
mds = sys.modules['sklearn'].manifold.MDS(n_components=n_components, n_init=n_init, max_iter=max_iter, eps=eps, random_state=random_state, dissimilarity="precomputed", n_jobs=n_jobs)
pos = mds.fit_transform(similarities)
# pos = mds.fit(similarities).embedding_
if debug:
print ' kmeans clustering with %d clusters' % n_clusters
kmeans = sys.modules['sklearn'].cluster.KMeans(n_clusters=n_clusters, random_state=random_state).fit(pos if pos is not None else similarities)
pcvals = {seqfos[iseq]['name'] : pos[iseq] if pos is not None else None for iseq in range(len(seqfos))}
labels = {seqfos[iseq]['name'] : kmeans.labels_[iseq] for iseq in range(len(seqfos))}
partition = utils.group_seqs_by_value(pcvals.keys(), lambda q: labels[q])
if plotdir is not None:
utils.prep_dir(plotdir, wildlings=['*.svg'])
if debug:
print ' plot'
plot_mds(n_components, pcvals, plotdir, 'mds', partition=partition)
if reco_info is not None:
labels = {uid : reco_info[uid][region + '_gene'] for uid in pcvals}
plot_mds(n_components, pcvals, plotdir, 'true-genes', labels=labels)
if debug:
print ' kmeans time %.1f' % (time.time() - start)
return partition
def plot(self, plotdir, only_csv=False):
print ' plotting performance',
import fraction_uncertainty
import plotting
start = time.time()
for substr in self.subplotdirs:
utils.prep_dir(plotdir + '/' + substr, wildlings=('*.csv', '*.svg'))
for column in self.values:
if column in plotconfig.gene_usage_columns:
right = self.values[column]['right']
wrong = self.values[column]['wrong']
lo, hi = fraction_uncertainty.err(right, right + wrong)
hist = plotting.make_bool_hist(right, wrong, self.name + '-' + column)
plotting.draw_no_root(hist, plotname=column, plotdir=plotdir + '/gene-call', write_csv=True, stats='0-bin', only_csv=only_csv)
else:
hist = plotting.make_hist_from_dict_of_counts(self.values[column], 'int', self.name + '-' + column, normalize=False)
if 'hamming_to_true_naive' in column:
xtitle = 'hamming distance'
tmpplotdir = plotdir + '/mutation'
else:
xtitle = 'inferred - true'
if 'muted' in column:
tmpplotdir = plotdir + '/mutation'
else:
tmpplotdir = plotdir + '/boundaries'
plotting.draw_no_root(hist, plotname=column, plotdir=tmpplotdir, write_csv=True, only_csv=only_csv, xtitle=xtitle, shift_overflows=True)
for column in self.hists:
if '_vs_mute_freq' in column or '_vs_per_gene_support' in column: # only really care about the fraction, which we plot below
continue
plotting.draw_no_root(self.hists[column], plotname=column, plotdir=plotdir + '/mutation', write_csv=True, only_csv=only_csv, ytitle='counts', xtitle='inferred - true', shift_overflows=True)
# fraction correct vs mute freq
for region in utils.regions:
hright = self.hists[region + '_gene_right_vs_mute_freq']
hwrong = self.hists[region + '_gene_wrong_vs_mute_freq']
if hright.integral(include_overflows=True) == 0:
continue
plotting.make_fraction_plot(hright, hwrong, plotdir + '/gene-call', region + '_fraction_correct_vs_mute_freq', xlabel='mut freq', ylabel='fraction correct up to allele', xbounds=(0., 0.5), only_csv=only_csv, write_csv=True)
# per-gene support stuff
for region in utils.regions:
if self.hists[region + '_allele_right_vs_per_gene_support'].integral(include_overflows=True) == 0:
continue
hright = self.hists[region + '_allele_right_vs_per_gene_support']
hwrong = self.hists[region + '_allele_wrong_vs_per_gene_support']
plotting.make_fraction_plot(hright, hwrong, plotdir + '/gene-call', region + '_allele_fraction_correct_vs_per_gene_support', xlabel='support', ylabel='fraction with correct allele', xbounds=(-0.1, 1.1), only_csv=only_csv, write_csv=True)
if not only_csv: # write html file and fix permissiions
for substr in self.subplotdirs:
plotting.make_html(plotdir + '/' + substr, n_columns=4)
print '(%.1f sec)' % (time.time()-start)
def __init__(self, args, glfo, seed, workdir, outfname): # NOTE <gldir> is not in general the same as <args.initial_germline_dir>
self.args = args
self.glfo = glfo
# NOTE in general *not* the same as <self.args.workdir> and <self.args.outfname>
self.workdir = workdir
self.outfname = outfname
utils.prep_dir(self.workdir)
# set <self.parameter_dir> (note that this is in general *not* the same as self.args.parameter_dir)
if self.args.rearrange_from_scratch: # currently not allowed to mutate from scratch without also rearranging from scratch (enforced in bin/partis)
if self.args.mutate_from_scratch:
self.parameter_dir = None
else:
self.parameter_dir = self.args.scratch_mute_freq_dir # if you make up mute freqs from scratch, unless you're really careful you tend to get nonsense results for a lot of things (e.g. allele finding). So it's easier to copy over a reasonable set of mut freq parameters from somewhere.
else:
self.parameter_dir = self.args.parameter_dir + '/' + self.args.parameter_type
self.index_keys = {} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
self.allele_prevalence_freqs = glutils.read_allele_prevalence_freqs(args.allele_prevalence_fname) if args.allele_prevalence_fname is not None else {}
self.version_freq_table = self.read_vdj_version_freqs() # list of the probabilities with which each VDJ combo (plus other rearrangement parameters) appears in data (none if rearranging from scratch)
self.insertion_content_probs = self.read_insertion_content() # dummy/uniform if rearranging from scratch
self.all_mute_freqs = {}
# read shm info NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with opener('r')(self.args.gtrfname) as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line['value']
treegen = treegenerator.TreeGenerator(args, self.parameter_dir, seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(seed, self.treefname)
with opener('r')(self.treefname) as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
os.remove(self.treefname)
if os.path.exists(self.outfname):
os.remove(self.outfname)
elif not os.path.exists(os.path.dirname(os.path.abspath(self.outfname))):
os.makedirs(os.path.dirname(os.path.abspath(self.outfname)))
def plot(self, plotdir, only_csv=False):
print ' plotting performance',
start = time.time()
for substr in self.subplotdirs:
utils.prep_dir(plotdir + '/' + substr, wildlings=('*.csv', '*.svg'))
for column in self.values:
if column in bool_columns:
right = self.values[column]['right']
wrong = self.values[column]['wrong']
lo, hi, _ = fraction_uncertainty.err(right, right + wrong)
hist = plotting.make_bool_hist(right, wrong, self.name + '-' + column)
plotting.draw_no_root(hist, plotname=column, plotdir=plotdir + '/gene-call', write_csv=True, stats='0-bin', only_csv=only_csv)
else:
hist = plotting.make_hist_from_dict_of_counts(self.values[column], 'int', self.name + '-' + column, normalize=False)
if 'hamming_to_true_naive' in column:
xtitle = 'hamming distance'
tmpplotdir = plotdir + '/mutation'
else:
xtitle = 'inferred - true'
if 'muted' in column:
tmpplotdir = plotdir + '/mutation'
else:
tmpplotdir = plotdir + '/boundaries'
plotting.draw_no_root(hist, plotname=column, plotdir=tmpplotdir, write_csv=True, only_csv=only_csv, xtitle=xtitle, shift_overflows=True)
for column in self.hists:
if '_vs_mute_freq' in column or '_vs_per_gene_support' in column: # only really care about the fraction, which we plot below
continue
plotting.draw_no_root(self.hists[column], plotname=column, plotdir=plotdir + '/mutation', write_csv=True, only_csv=only_csv, ytitle='counts', xtitle='inferred - true', shift_overflows=True)
# fraction correct vs mute freq
for region in utils.regions:
hright = self.hists[region + '_gene_right_vs_mute_freq']
hwrong = self.hists[region + '_gene_wrong_vs_mute_freq']
if hright.integral(include_overflows=True) == 0:
continue
plotting.make_fraction_plot(hright, hwrong, plotdir + '/gene-call', region + '_fraction_correct_vs_mute_freq', xlabel='mut freq', ylabel='fraction correct up to allele', xbounds=(0., 0.5), only_csv=only_csv, write_csv=True)
# per-gene support stuff
for region in utils.regions:
if self.hists[region + '_allele_right_vs_per_gene_support'].integral(include_overflows=True) == 0:
continue
hright = self.hists[region + '_allele_right_vs_per_gene_support']
hwrong = self.hists[region + '_allele_wrong_vs_per_gene_support']
plotting.make_fraction_plot(hright, hwrong, plotdir + '/gene-call', region + '_allele_fraction_correct_vs_per_gene_support', xlabel='support', ylabel='fraction with correct allele', xbounds=(-0.1, 1.1), only_csv=only_csv, write_csv=True)
if not only_csv: # write html file and fix permissiions
for substr in self.subplotdirs:
plotting.make_html(plotdir + '/' + substr, n_columns=4)
print '(%.1f sec)' % (time.time()-start)
def make_mean_plots(plotdir, subdirs, outdir):
meanlist, variancelist = [], []
normalized_means = []
for sd in subdirs:
with opener('r')(plotdir + '/' + sd + '/plots/means.csv') as meanfile:
reader = csv.DictReader(meanfile)
for line in reader:
means = [float(m) for m in line['means'].split(':')]
meanlist.append(numpy.mean(means))
variancelist.append(numpy.var(means))
nmvals = [
float(nm) for nm in line['normalized-means'].split(':')
]
normalized_means += nmvals
import matplotlib
matplotlib.use('Agg')
from matplotlib import pyplot
# ----------------------------------------------------------------------------------------
# first make hexbin plot
pyplot.subplot(111)
pyplot.hexbin(meanlist,
variancelist,
gridsize=20,
cmap=matplotlib.cm.gist_yarg,
bins=None)
# pyplot.axis([0, 5, 0, 2])
pyplot.xlabel('mean')
pyplot.ylabel('variance')
cb = pyplot.colorbar()
cb.set_label('mean value')
utils.prep_dir(outdir + '/plots', multilings=['*.png', '*.svg', '*.csv'])
pyplot.savefig(outdir + '/plots/hexmeans.png')
pyplot.clf()
# ----------------------------------------------------------------------------------------
# then make normalized mean plot
n, bins, patches = pyplot.hist(normalized_means, 50)
pyplot.xlabel(r'$(x_i - \mu) / \sigma_i$')
pyplot.title(r'$\sigma=' + str(math.sqrt(numpy.var(normalized_means))) +
'$')
# pyplot.axis([-10, 10, 0, 220])
pyplot.savefig(outdir + '/plots/means.png')
check_call(
['./permissify-www', outdir]
) # NOTE this should really permissify starting a few directories higher up
def plot(self,
plotdir,
partition=None,
infiles=None,
annotations=None,
only_csv=None):
import plotting
print ' plotting partitions'
sys.stdout.flush()
start = time.time()
for subdir in self.subplotdirs:
utils.prep_dir(plotdir + '/' + subdir,
wildlings=['*.csv', '*.svg'])
fnames = []
if partition is not None: # one partition
assert infiles is None
assert annotations is not None
csize_hists = {'best': plotting.get_cluster_size_hist(partition)}
# self.plot_within_vs_between_hists(partition, annotations, plotdir)
fnames += self.plot_size_vs_shm(partition, annotations, plotdir)
elif infiles is not None: # plot the mean of a partition from each file
subset_hists = []
for fname in infiles:
cp = ClusterPath()
cp.readfile(fname)
subset_hists.append(
plotting.get_cluster_size_hist(cp.partitions[cp.i_best]))
csize_hists = {'best': plotting.make_mean_hist(subset_hists)}
for ih in range(len(subset_hists)):
subset_hists[ih].write(plotdir +
('/subset-%d-cluster-sizes.csv' % ih))
else:
assert False
plotting.plot_cluster_size_hists(plotdir +
'/overall/cluster-sizes.svg',
csize_hists,
title='',
log='x')
fnames.append(['cluster-sizes.svg'])
if not only_csv:
for subdir in self.subplotdirs:
plotting.make_html(plotdir + '/' + subdir,
fnames=fnames,
new_table_each_row=True)
print '(%.1f sec)' % (time.time() - start)
def write(self, base_outdir):
print ' writing parameters'
start = time.time()
utils.prep_dir(base_outdir, multilings=('*.csv', '*.svg'))
mute_start = time.time()
self.mutefreqer.write(
base_outdir,
mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv'
) # REGION is replace by each region in the three output files)
print ' mut freq write time: %.3f' % (time.time() - mute_start)
# print ' %d / %d cached' % (self.mutefreqer.n_cached, self.mutefreqer.n_cached + self.mutefreqer.n_not_cached)
for column in self.counts:
index = None
outfname = None
if column == 'all':
index = utils.index_columns
outfname = base_outdir + '/' + utils.get_parameter_fname(
column='all')
elif '_content' in column:
index = [
column,
]
outfname = base_outdir + '/' + column + '.csv'
else:
index = [
column,
] + utils.column_dependencies[column]
outfname = base_outdir + '/' + utils.get_parameter_fname(
column_and_deps=index)
if os.path.isfile(outfname):
os.remove(outfname)
elif not os.path.exists(base_outdir):
os.makedirs(base_outdir)
with opener('w')(outfname) as outfile:
out_fieldnames = list(index)
out_fieldnames.append('count')
out_data = csv.DictWriter(outfile, out_fieldnames)
out_data.writeheader()
# NOTE this will in general not be sorted
for key, count in self.counts[column].iteritems():
line = {}
for ic in range(len(key)):
line[index[ic]] = key[ic]
line['count'] = count
out_data.writerow(line)
print ' parameter write time: %.3f' % (time.time() - start)
def compare_directories(args, plotdirlist, outdir):
utils.prep_dir(outdir, wildlings=['*.png', '*.svg', '*.csv'])
# read hists from <plotdirlist>
allhists = OrderedDict()
allvars = set() # all variables that appeared in any dir
for idir in range(len(plotdirlist)):
dirhists = get_hists_from_dir(plotdirlist[idir], args.names[idir])
allvars |= set(dirhists.keys())
allhists[args.names[idir]] = dirhists
# then loop over all the <varname>s we found
for varname in allvars:
hlist = [allhists[dname].get(varname, Hist(1, 0, 1, title='null')) for dname in allhists]
plot_single_variable(args, varname, hlist, outdir, pathnameclues=plotdirlist[0])
plotting.make_html(outdir, n_columns=4)
def compare_directories(args, plotdirlist, outdir):
utils.prep_dir(outdir, wildlings=['*.png', '*.svg', '*.csv'])
# read hists from <plotdirlist>
allhists = OrderedDict()
allvars = set() # all variables that appeared in any dir
for idir in range(len(plotdirlist)):
dirhists = get_hists_from_dir(plotdirlist[idir], args.names[idir])
allvars |= set(dirhists.keys())
allhists[args.names[idir]] = dirhists
# then loop over all the <varname>s we found
for varname in allvars:
hlist = [allhists[dname].get(varname, Hist(1, 0, 1, title='null')) for dname in allhists]
plot_single_variable(args, varname, hlist, outdir, pathnameclues=plotdirlist[0])
plotting.make_html(outdir, n_columns=4)
def kmeans_cluster_v_seqs(self, qr_seqs, swfo, plotdir=None, debug=False):
if plotdir is not None:
utils.prep_dir(plotdir, wildlings=['*.svg'], subdirs=[d for d in os.listdir(plotdir) if os.path.isdir(plotdir + '/' + d)], rm_subdirs=True)
clusterfos = []
if debug:
print 'kmeans clustering'
print ' seqs family'
for family, seqfos in self.get_family_groups(qr_seqs, swfo).items():
if debug:
print ' %5d %s' % (len(seqfos), family)
partition = mds.bios2mds_kmeans_cluster(self.n_mds_components, self.XXX_n_kmeans_clusters, seqfos, self.args.workdir + '/mds', self.args.seed, reco_info=self.reco_info, region=self.region, plotdir=plotdir + '/' + family if plotdir is not None else None)
# partition = mds.run_sklearn_mds(self.n_mds_components, self.XXX_n_kmeans_clusters, seqfos, self.args.seed, reco_info=self.reco_info, region=self.region, plotdir=plotdir + '/' + family if plotdir is not None else None)
clusterfos += self.get_clusterfos_from_partition(partition, qr_seqs)
clusterfos = sorted(clusterfos, key=lambda c: len(c['seqfos']), reverse=True)
return clusterfos
def __init__(self, args, base_plotdir):
self.base_plotdir = base_plotdir
self.eps_to_skip = 1e-3
print 'skipping eps %f' % self.eps_to_skip
plot_types = ('transitions', 'emissions')
for ptype in plot_types:
plotdir = self.base_plotdir + '/' + ptype
utils.prep_dir(plotdir, wildlings=['*.png', '*.svg'])
if args.hmmdir != None:
self.filelist = glob.glob(args.hmmdir + '/*.yaml')
else:
self.filelist = utils.get_arg_list(args.infiles)
if len(self.filelist) == 0:
raise Exception('zero files passed to modelplotter')
def write(self, base_outdir, mean_freq_outfname):
if not self.finalized:
self.finalize()
outdir = base_outdir + '/mute-freqs'
utils.prep_dir(outdir, '*.csv')
for gene in self.counts:
counts, freqs, plotting_info = self.counts[gene], self.freqs[
gene], self.plotting_info[gene]
sorted_positions = sorted(counts)
outfname = outdir + '/' + utils.sanitize_name(gene) + '.csv'
with opener('w')(outfname) as outfile:
nuke_header = []
for nuke in utils.nukes:
nuke_header.append(nuke)
nuke_header.append(nuke + '_obs')
nuke_header.append(nuke + '_lo_err')
nuke_header.append(nuke + '_hi_err')
writer = csv.DictWriter(
outfile, ('position', 'mute_freq', 'lo_err', 'hi_err') +
tuple(nuke_header))
writer.writeheader()
for position in sorted_positions:
row = {
'position': position,
'mute_freq': counts[position]['freq'],
'lo_err': counts[position]['freq_lo_err'],
'hi_err': counts[position]['freq_hi_err']
}
for nuke in utils.nukes:
row[nuke] = freqs[position][nuke]
row[nuke + '_obs'] = counts[position][nuke]
row[nuke + '_lo_err'] = freqs[position][nuke +
'_lo_err']
row[nuke + '_hi_err'] = freqs[position][nuke +
'_hi_err']
writer.writerow(row)
assert 'REGION' in mean_freq_outfname
self.mean_rates['all'].write(
mean_freq_outfname.replace(
'REGION', 'all')) # hackey hackey hackey replacement... *sigh*
for region in utils.regions:
self.mean_rates[region].write(
mean_freq_outfname.replace('REGION', region))
def __init__(self, args, glfo, seed, workdir): # NOTE <gldir> is not in general the same as <args.initial_germline_dir> # rm workdir
self.args = args
self.glfo = glfo
if len(glfo['seqs']['v']) > 100: # this is kind of a shitty criterion, but I don't know what would be better (we basically just want to warn people if they're simulating from data/germlines/human)
print ' note: simulating with a very large number (%d) of V genes (the use of realistic diploid sets can be controlled either by using inferred germline sets that you\'ve got lying around (--reco-parameter-dir), or with --generate-germline-set)' % len(glfo['seqs']['v'])
self.workdir = tempfile.mkdtemp()
utils.prep_dir(self.workdir)
assert self.args.parameter_dir is None
self.reco_parameter_dir = self.args.reco_parameter_dir + '/' + self.args.parameter_type if self.args.reco_parameter_dir is not None else None
self.shm_parameter_dir = self.args.shm_parameter_dir + '/' + self.args.parameter_type if self.args.shm_parameter_dir is not None else None
self.index_keys = {} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
self.allele_prevalence_freqs = glutils.read_allele_prevalence_freqs(args.allele_prevalence_fname) if args.allele_prevalence_fname is not None else {}
self.version_freq_table = self.read_vdj_version_freqs() # list of the probabilities with which each VDJ combo (plus other rearrangement parameters) appears in data (none if rearranging from scratch)
self.insertion_content_probs = self.read_insertion_content() # dummy/uniform if rearranging from scratch
self.all_mute_freqs = {}
# read shm info NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with open(self.args.gtrfname, 'r') as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line['value']
treegen = treegenerator.TreeGenerator(args, self.shm_parameter_dir, seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(seed, self.treefname, self.workdir) # NOTE not really a newick file, since I hack on the per-region branch length info at the end of each line
with open(self.treefname, 'r') as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
os.remove(self.treefname)
self.validation_values = {'heights' : {t : {'in' : [], 'out' : []} for t in ['all'] + utils.regions}}
def write(self, base_outdir):
print " writing parameters",
sys.stdout.flush()
start = time.time()
utils.prep_dir(
base_outdir, subdirs=("hmms", "mute-freqs", "germline-sets"), wildlings=("*.csv", "*.yaml", "*.fasta")
) # it's kind of hackey to specify the /hmms dir here, but as soon as we write the parameters below, the previous yamels are out of date, so it's pretty much necessary
self.mfreqer.write(
base_outdir + "/mute-freqs", mean_freq_outfname=base_outdir + "/REGION-mean-mute-freqs.csv"
) # REGION is replace by each region in the three output files)
genes_with_counts = [g[0] for r in utils.regions for g in self.counts[r + "_gene"].keys()]
glutils.write_glfo(base_outdir + "/" + glutils.glfo_dir, self.glfo, only_genes=genes_with_counts, debug=False)
for column in self.counts:
index = None
outfname = None
if column == "all":
index = tuple(list(utils.index_columns) + ["cdr3_length"])
outfname = base_outdir + "/" + utils.get_parameter_fname(column="all")
elif "_content" in column:
index = [column]
outfname = base_outdir + "/" + column + ".csv"
else:
index = [column] + utils.column_dependencies[column]
outfname = base_outdir + "/" + utils.get_parameter_fname(column_and_deps=index)
if os.path.isfile(outfname):
os.remove(outfname)
elif not os.path.exists(base_outdir):
os.makedirs(base_outdir)
with opener("w")(outfname) as outfile:
out_fieldnames = list(index)
out_fieldnames.append("count")
out_data = csv.DictWriter(outfile, out_fieldnames)
out_data.writeheader()
# NOTE this will in general not be sorted
for key, count in self.counts[column].iteritems():
line = {}
for ic in range(len(key)):
line[index[ic]] = key[ic]
line["count"] = count
out_data.writerow(line)
print "(%.1f sec)" % (time.time() - start)
def run_sklearn_mds(n_components, n_clusters, seqfos, seed, reco_info=None, region=None, aligned=False, n_init=4, max_iter=300, eps=1e-3, n_jobs=-1, plotdir=None):
print '%s not testing this after moving these imports down here' % utils.color('red', 'hey')
from sklearn import manifold # these are both slow af to import, even on local ssd
from sklearn.cluster import KMeans
if len(set(sfo['name'] for sfo in seqfos)) != len(seqfos):
raise Exception('duplicate sequence ids in <seqfos>')
print 'align'
if not aligned: # NOTE unlike the bios2mds version above, this modifies <seqfos>
seqfos = utils.align_many_seqs(seqfos)
print ' distances'
# translations = string.maketrans('ACGT-', '01234')
# def convert(seq):
# return [int(c) for c in seq.translate(translations)]
# converted_seqs = [convert(x['seq']) for x in seqfos]
# similarities = scipy.spatial.distance.pdist(converted_seqs, 'hamming')
# similarities = scipy.spatial.distance.squareform(similarities)
similarities = scipy.spatial.distance.squareform([utils.hamming_fraction(seqfos[i]['seq'], seqfos[j]['seq']) for i in range(len(seqfos)) for j in range(i + 1, len(seqfos))])
print ' mds'
random_state = numpy.random.RandomState(seed=seed)
mds = manifold.MDS(n_components=n_components, n_init=n_init, max_iter=max_iter, eps=eps, random_state=random_state, dissimilarity="precomputed", n_jobs=n_jobs)
pos = mds.fit_transform(similarities)
# pos = mds.fit(similarities).embedding_
print ' kmeans'
kmeans = KMeans(n_clusters=n_clusters, random_state=random_state).fit(pos)
pcvals = {seqfos[iseq]['name'] : pos[iseq] for iseq in range(len(seqfos))}
labels = {seqfos[iseq]['name'] : kmeans.labels_[iseq] for iseq in range(len(seqfos))}
def keyfunc(q): # should really integrate this with utils.collapse_naive_seqs()/utils.split_partition_with_criterion()
return labels[q]
partition = [list(group) for _, group in itertools.groupby(sorted(pcvals, key=keyfunc), key=keyfunc)]
if plotdir is not None:
utils.prep_dir(plotdir, wildlings=['*.svg'])
print ' plot'
plot_mds(n_components, pcvals, plotdir, 'mds', partition=partition)
if reco_info is not None:
labels = {uid : reco_info[uid][region + '_gene'] for uid in pcvals}
plot_mds(n_components, pcvals, plotdir, 'true-genes', labels=labels)
return partition
def make_mean_plots(plotdir, subdirs, outdir):
meanlist, variancelist = [], []
normalized_means = []
for sd in subdirs:
with opener('r')(plotdir + '/' + sd + '/plots/means.csv') as meanfile:
reader = csv.DictReader(meanfile)
for line in reader:
means = [ float(m) for m in line['means'].split(':') ]
meanlist.append(numpy.mean(means))
variancelist.append(numpy.var(means))
nmvals = [ float(nm) for nm in line['normalized-means'].split(':') ]
normalized_means += nmvals
import matplotlib
matplotlib.use('Agg')
from matplotlib import pyplot
# ----------------------------------------------------------------------------------------
# first make hexbin plot
pyplot.subplot(111)
pyplot.hexbin(meanlist, variancelist, gridsize=20, cmap=matplotlib.cm.gist_yarg, bins=None)
# pyplot.axis([0, 5, 0, 2])
pyplot.xlabel('mean')
pyplot.ylabel('variance')
cb = pyplot.colorbar()
cb.set_label('mean value')
utils.prep_dir(outdir + '/plots', multilings=['*.png', '*.svg', '*.csv'])
pyplot.savefig(outdir + '/plots/hexmeans.png')
pyplot.clf()
# ----------------------------------------------------------------------------------------
# then make normalized mean plot
n, bins, patches = pyplot.hist(normalized_means, 50)
pyplot.xlabel(r'$(x_i - \mu) / \sigma_i$')
pyplot.title(r'$\sigma=' + str(math.sqrt(numpy.var(normalized_means))) + '$')
# pyplot.axis([-10, 10, 0, 220])
pyplot.savefig(outdir + '/plots/means.png')
check_call(['./permissify-www', outdir]) # NOTE this should really permissify starting a few directories higher up
def write(self, base_outdir):
print ' writing parameters',
sys.stdout.flush()
start = time.time()
utils.prep_dir(base_outdir, subdirs=('hmms', 'mute-freqs', 'germline-sets'), wildlings=('*.csv', '*.yaml', '*.fasta')) # it's kind of hackey to specify the /hmms dir here, but as soon as we write the parameters below, the previous yamels are out of date, so it's pretty much necessary
self.mfreqer.write(base_outdir + '/mute-freqs', mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv') # REGION is replace by each region in the three output files)
genes_with_counts = [g[0] for r in utils.regions for g in self.counts[r + '_gene'].keys()]
glutils.write_glfo(base_outdir + '/' + glutils.glfo_dir, self.glfo, only_genes=genes_with_counts, debug=False)
for column in self.counts:
index = None
outfname = None
if column == 'all':
index = tuple(list(utils.index_columns) + ['cdr3_length', ])
outfname = base_outdir + '/' + utils.get_parameter_fname(column='all')
elif '_content' in column:
index = [column,]
outfname = base_outdir + '/' + column + '.csv'
else:
index = [column,] + utils.column_dependencies[column]
outfname = base_outdir + '/' + utils.get_parameter_fname(column_and_deps=index)
if os.path.isfile(outfname):
os.remove(outfname)
elif not os.path.exists(base_outdir):
os.makedirs(base_outdir)
with open(outfname, 'w') as outfile:
out_fieldnames = list(index)
out_fieldnames.append('count')
out_data = csv.DictWriter(outfile, out_fieldnames)
out_data.writeheader()
# NOTE this will in general not be sorted
for key, count in self.counts[column].iteritems():
line = {}
for ic in range(len(key)):
line[index[ic]] = key[ic]
line['count'] = count
out_data.writerow(line)
print '(%.1f sec)' % (time.time()-start)
def make_tree(all_genes, workdir, use_cache=False):
aligned_fname = workdir + '/all-aligned.fa'
raxml_label = 'xxx'
raxml_output_fnames = [
'%s/RAxML_%s.%s' % (workdir, fn, raxml_label)
for fn in ['parsimonyTree', 'log', 'result', 'info', 'bestTree']
]
treefname = [fn for fn in raxml_output_fnames if 'result' in fn][0]
if use_cache: # don't re-run muxcle & raxml, just use the previous run's output tree file
return treefname
utils.prep_dir(workdir,
wildlings=[
'*.' + raxml_label,
os.path.basename(aligned_fname), 'out', 'err',
os.path.basename(aligned_fname) + '.reduced'
])
# write and align an .fa with all alleles from any gl set
with tempfile.NamedTemporaryFile() as tmpfile:
for name, seq in all_genes.items():
tmpfile.write('>%s\n%s\n' % (name, seq))
tmpfile.flush() # BEWARE if you forget this you are f****d
cmdstr = '%s -in %s -out %s' % (args.muscle_path, tmpfile.name,
aligned_fname)
print ' %s %s' % (utils.color('red', 'run'), cmdstr)
utils.run_cmds(get_cmdfos(cmdstr, workdir, aligned_fname),
ignore_stderr=True)
# get a tree for the aligned .fa
cmdstr = '%s -mGTRCAT -n%s -s%s -p1 -w%s' % (args.raxml_path, raxml_label,
aligned_fname, workdir)
print ' %s %s' % (utils.color('red', 'run'), cmdstr)
utils.run_cmds(get_cmdfos(cmdstr, workdir, treefname), ignore_stderr=True)
os.remove(aligned_fname) # rm muscle output
for fn in [
f for f in raxml_output_fnames if f != treefname
]: # rm all the raxml outputs except what the one file we really want
os.remove(fn)
return treefname
def write(self, base_outdir, my_datadir=None):
print ' writing parameters',
sys.stdout.flush()
start = time.time()
utils.prep_dir(base_outdir, subdirs=('hmms', 'mute-freqs', 'germline-sets'), wildlings=('*.csv', '*.yaml', '*.fasta')) # it's kind of hackey to specify the /hmms dir here, but as soon as we write the parameters below, the previous yamels are out of date, so it's pretty much necessary
self.mfreqer.write(base_outdir + '/mute-freqs', mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv') # REGION is replace by each region in the three output files)
genes_with_counts = [g[0] for r in utils.regions for g in self.counts[r + '_gene'].keys()]
glutils.write_glfo(base_outdir + '/' + glutils.glfo_dir, self.glfo, only_genes=genes_with_counts, debug=True)
for column in self.counts:
index = None
outfname = None
if column == 'all':
index = tuple(list(utils.index_columns) + ['cdr3_length', ])
outfname = base_outdir + '/' + utils.get_parameter_fname(column='all')
elif '_content' in column:
index = [column,]
outfname = base_outdir + '/' + column + '.csv'
else:
index = [column,] + utils.column_dependencies[column]
outfname = base_outdir + '/' + utils.get_parameter_fname(column_and_deps=index)
if os.path.isfile(outfname):
os.remove(outfname)
elif not os.path.exists(base_outdir):
os.makedirs(base_outdir)
with opener('w')(outfname) as outfile:
out_fieldnames = list(index)
out_fieldnames.append('count')
out_data = csv.DictWriter(outfile, out_fieldnames)
out_data.writeheader()
# NOTE this will in general not be sorted
for key, count in self.counts[column].iteritems():
line = {}
for ic in range(len(key)):
line[index[ic]] = key[ic]
line['count'] = count
out_data.writerow(line)
print '(%.1f sec)' % (time.time()-start)
def plot(self, base_plotdir, cyst_positions=None, tryp_positions=None, only_csv=False):
if not self.finalized:
self.finalize()
plotdir = base_plotdir + '/mute-freqs'
utils.prep_dir(plotdir + '/plots', multilings=('*.csv', '*.svg'))
for region in utils.regions:
utils.prep_dir(plotdir + '/' + region + '/plots', multilings=('*.csv', '*.svg'))
# utils.prep_dir(plotdir + '/' + region + '-per-base/plots', multilings=('*.csv', '*.png'))
for gene in self.counts:
counts, plotting_info = self.counts[gene], self.plotting_info[gene]
sorted_positions = sorted(counts)
genehist = Hist(sorted_positions[-1] - sorted_positions[0] + 1, sorted_positions[0] - 0.5, sorted_positions[-1] + 0.5, xtitle='fixme', ytitle='fixme') #, title=utils.sanitize_name(gene))
for position in sorted_positions:
hi_diff = abs(counts[position]['freq'] - counts[position]['freq_hi_err'])
lo_diff = abs(counts[position]['freq'] - counts[position]['freq_lo_err'])
err = 0.5*(hi_diff + lo_diff)
genehist.set_ibin(genehist.find_bin(position), counts[position]['freq'], error=err)
xline = None
figsize = [3, 3]
if utils.get_region(gene) == 'v' and cyst_positions is not None:
xline = cyst_positions[gene]['cysteine-position']
figsize[0] *= 3.5
elif utils.get_region(gene) == 'j' and tryp_positions is not None:
xline = int(tryp_positions[gene])
figsize[0] *= 2
plotting.draw_no_root(genehist, plotdir=plotdir + '/' + utils.get_region(gene), plotname=utils.sanitize_name(gene), errors=True, write_csv=True, xline=xline, figsize=figsize, only_csv=only_csv)
# paramutils.make_mutefreq_plot(plotdir + '/' + utils.get_region(gene) + '-per-base', utils.sanitize_name(gene), plotting_info) # needs translation to mpl
# make mean mute freq hists
plotting.draw_no_root(self.mean_rates['all'], plotname='all-mean-freq', plotdir=plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True, only_csv=only_csv)
for region in utils.regions:
plotting.draw_no_root(self.mean_rates[region], plotname=region+'-mean-freq', plotdir=plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True, only_csv=only_csv)
if not only_csv: # write html file and fix permissiions
check_call(['./bin/makeHtml', plotdir, '3', 'null', 'svg'])
for region in utils.regions:
check_call(['./bin/makeHtml', plotdir + '/' + region, '1', 'null', 'svg'])
# check_call(['./bin/makeHtml', plotdir + '/' + region + '-per-base', '1', 'null', 'png'])
check_call(['./bin/permissify-www', plotdir]) # NOTE this should really permissify starting a few directories higher up
def write_vdjalign_input(self, base_infname, n_procs):
queries_per_proc = float(len(self.remaining_queries)) / n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
if n_procs == 1: # double check for rounding problems or whatnot
assert n_queries_per_proc == len(self.remaining_queries)
for iproc in range(n_procs):
workdir = self.args.workdir
if n_procs > 1:
workdir += '/sw-' + str(iproc)
utils.prep_dir(workdir)
with opener('w')(workdir + '/' + base_infname) as sub_infile:
for iquery in range(iproc*n_queries_per_proc, (iproc + 1)*n_queries_per_proc):
if iquery >= len(self.remaining_queries):
break
query_name = self.remaining_queries[iquery]
sub_infile.write('>' + query_name + ' NUKES\n')
seq = self.input_info[query_name]['seq']
if query_name in self.info['indels']:
seq = self.info['indels'][query_name]['reversed_seq'] # use the query sequence with shm insertions and deletions reversed
sub_infile.write(seq + '\n')
def write(self, base_outdir):
print ' writing parameters',
sys.stdout.flush()
start = time.time()
utils.prep_dir(base_outdir, multilings=('*.csv', '*.svg'))
# mute_start = time.time()
self.mutefreqer.write(base_outdir, mean_freq_outfname=base_outdir + '/REGION-mean-mute-freqs.csv') # REGION is replace by each region in the three output files)
# print ' mut freq write time: %.3f' % (time.time() - mute_start)
# print ' %d / %d cached' % (self.mutefreqer.n_cached, self.mutefreqer.n_cached + self.mutefreqer.n_not_cached)
for column in self.counts:
index = None
outfname = None
if column == 'all':
index = utils.index_columns
outfname = base_outdir + '/' + utils.get_parameter_fname(column='all')
elif '_content' in column:
index = [column,]
outfname = base_outdir + '/' + column + '.csv'
else:
index = [column,] + utils.column_dependencies[column]
outfname = base_outdir + '/' + utils.get_parameter_fname(column_and_deps=index)
if os.path.isfile(outfname):
os.remove(outfname)
elif not os.path.exists(base_outdir):
os.makedirs(base_outdir)
with opener('w')(outfname) as outfile:
out_fieldnames = list(index)
out_fieldnames.append('count')
out_data = csv.DictWriter(outfile, out_fieldnames)
out_data.writeheader()
# NOTE this will in general not be sorted
for key, count in self.counts[column].iteritems():
line = {}
for ic in range(len(key)):
line[index[ic]] = key[ic]
line['count'] = count
out_data.writerow(line)
print '(%.1f sec)' % (time.time()-start)
def split_input(self, n_procs, infname=None, info=None, prefix='sub'):
"""
If <infname> is specified split the csv info from it into <n_procs> input files in subdirectories labelled with '<prefix>-' within <self.args.workdir>
If <info> is specified, instead split the list <info> into pieces and return a list of the resulting lists
"""
if info is None:
assert infname is not None
info = []
with opener('r')(infname) as infile:
reader = csv.DictReader(infile)
for line in reader:
info.append(line)
else:
assert infname is None # make sure only *one* of 'em is specified
outlists = []
queries_per_proc = float(len(info)) / n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
for iproc in range(n_procs):
if infname is None:
outlists.append([])
else:
subworkdir = self.args.workdir + '/' + prefix + '-' + str(
iproc)
utils.prep_dir(subworkdir)
sub_outfile = opener('w')(subworkdir + '/' +
os.path.basename(infname))
writer = csv.DictWriter(sub_outfile, reader.fieldnames)
writer.writeheader()
for iquery in range(iproc * n_queries_per_proc,
(iproc + 1) * n_queries_per_proc):
if iquery >= len(info):
break
if infname is None:
outlists[-1].append(info[iquery])
else:
writer.writerow(info[iquery])
if infname is None:
return outlists
def finalize_tigger(self):
utils.prep_dir(os.getenv('www') + '/partis/tmp', wildling='*.svg')
for gene in self.counts:
if utils.get_region(gene) != 'v':
continue
print '\n%s' % gene
print ' position x-icpt y-icpt slope mut / total'
mean_x_icpt = {'sum' : 0., 'total' : 0.}
for position in sorted(self.counts[gene].keys()):
self.freqs[gene][position]['tigger'] = self.tigger_calcs(position, self.counts[gene][position], mean_x_icpt)
print mean_x_icpt
if mean_x_icpt['total'] > 0.:
print mean_x_icpt['sum'] / mean_x_icpt['total']
assert False
for gene in self.freqs:
if utils.get_region(gene) != 'v':
continue
info = {p : self.freqs[gene][p]['tigger-fits'] for p in self.freqs[gene]}
x_intercepts = [-v['intercept'] / v['slope'] for k, v in info.items() if v['intercept'] is not None and v['intercept'] < 0.3]
print sorted(x_intercepts)
print sum(x_intercepts) / float(len(x_intercepts))
print numpy.median(x_intercepts)
def write_vdjalign_input(self, base_infname, n_procs):
queries_per_proc = float(len(self.remaining_queries)) / n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
if n_procs == 1: # double check for rounding problems or whatnot
assert n_queries_per_proc == len(self.remaining_queries)
for iproc in range(n_procs):
workdir = self.args.workdir
if n_procs > 1:
workdir += '/sw-' + str(iproc)
utils.prep_dir(workdir)
with opener('w')(workdir + '/' + base_infname) as sub_infile:
for iquery in range(iproc * n_queries_per_proc,
(iproc + 1) * n_queries_per_proc):
if iquery >= len(self.remaining_queries):
break
query_name = self.remaining_queries[iquery]
sub_infile.write('>' + query_name + ' NUKES\n')
seq = self.input_info[query_name]['seq']
if query_name in self.info['indels']:
seq = self.info['indels'][query_name][
'reversed_seq'] # use the query sequence with shm insertions and deletions reversed
sub_infile.write(seq + '\n')
def __init__(self, germlines, plotdir, name):
self.germlines = germlines
self.plotdir = plotdir
self.name = name
utils.prep_dir(self.plotdir + '/plots', wildling=None, multilings=['*.csv', '*.svg', '*.root'])
self.values = {}
for column in utils.index_columns:
if column == 'cdr3_length': # kind of finicky to figure out what this is, so I don't always set it
continue
self.values[column] = {}
if column in bool_columns:
self.values[column]['right'] = 0
self.values[column]['wrong'] = 0
self.values['hamming_to_true_naive'] = {}
for region in utils.regions:
self.values[region + '_hamming_to_true_naive'] = {}
self.values[region + '_hamming_to_true_naive_normed'] = {}
# for bound in utils.boundaries:
# self.counts[bound + '_insertion_content'] = {'A':0, 'C':0, 'G':0, 'T':0} # base content of each insertion
# self.counts['seq_content'] = {'A':0, 'C':0, 'G':0, 'T':0}
# n_bins, xmin, xmax = 100, 0.0, 1.0
self.hists = {}
self.hists['mute_freqs'] = Hist(30, -0.05, 0.05)
def __init__(self, args, base_plotdir):
self.base_plotdir = base_plotdir
self.eps_to_skip = 1e-3
print 'skipping eps %f' % self.eps_to_skip
plot_types = ('transitions', 'emissions')
for ptype in plot_types:
plotdir = self.base_plotdir + '/' + ptype
utils.prep_dir(plotdir, wildlings=['*.png', '*.svg'])
if args.hmmdir != None:
filelist = glob.glob(args.hmmdir + '/*.yaml')
else:
filelist = utils.get_arg_list(args.infiles)
if len(filelist) == 0:
raise Exception('zero files passed to modelplotter')
for infname in filelist:
gene_name = os.path.basename(infname).replace('.yaml', '') # the sanitized name, actually
with open(infname) as infile:
model = yaml.load(infile)
self.make_transition_plot(gene_name, model)
self.make_emission_plot(gene_name, model)
def write_vdjalign_input(self, base_infname):
# first make a list of query names so we can iterate over an ordered collection
ordered_info = []
for query_name in self.input_info:
ordered_info.append(query_name)
queries_per_proc = float(len(self.input_info)) / self.args.n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
if self.args.n_procs == 1: # double check for rounding problems or whatnot
assert n_queries_per_proc == len(self.input_info)
for iproc in range(self.args.n_procs):
workdir = self.args.workdir
if self.args.n_procs > 1:
workdir += '/sw-' + str(iproc)
utils.prep_dir(workdir)
infname = workdir + '/' + base_infname
with opener('w')(workdir + '/' + base_infname) as sub_infile:
for iquery in range(iproc*n_queries_per_proc, (iproc + 1)*n_queries_per_proc):
if iquery >= len(ordered_info):
break
query_name = ordered_info[iquery]
sub_infile.write('>' + str(query_name) + ' NUKES\n')
sub_infile.write(self.input_info[query_name]['seq'] + '\n')
def write_hmms(self, parameter_dir, sw_matches):
print 'writing hmms with info from %s' % parameter_dir
start = time.time()
from hmmwriter import HmmWriter
hmm_dir = parameter_dir + '/hmms'
utils.prep_dir(hmm_dir, '*.yaml')
gene_list = self.args.only_genes
if gene_list == None: # if specific genes weren't specified, do the ones for which we have matches
gene_list = []
for region in utils.regions:
for gene in self.germline_seqs[region]:
if sw_matches == None or gene in sw_matches: # shouldn't be None really, but I'm testing something
gene_list.append(gene)
for gene in gene_list:
if self.args.debug:
print ' %s' % utils.color_gene(gene)
writer = HmmWriter(parameter_dir, hmm_dir, gene, self.args.naivety,
self.germline_seqs[utils.get_region(gene)][gene],
self.args)
writer.write()
print ' time to write hmms: %.3f' % (time.time()-start)
def __init__(self, args, base_plotdir):
self.base_plotdir = base_plotdir
self.eps_to_skip = 1e-3
print 'skipping eps %f' % self.eps_to_skip
plot_types = ('transitions', 'emissions')
for ptype in plot_types:
plotdir = self.base_plotdir + '/' + ptype
utils.prep_dir(plotdir, wildlings=['*.png', '*.svg'])
if args.hmmdir != None:
filelist = glob.glob(args.hmmdir + '/*.yaml')
else:
filelist = utils.get_arg_list(args.infiles)
if len(filelist) == 0:
raise Exception('zero files passed to modelplotter')
for infname in filelist:
gene_name = os.path.basename(infname).replace(
'.yaml', '') # the sanitized name, actually
with open(infname) as infile:
model = yaml.load(infile)
self.make_transition_plot(gene_name, model)
self.make_emission_plot(gene_name, model)
def write_hmms(self, parameter_dir, sw_matches):
print 'writing hmms with info from %s' % parameter_dir
start = time.time()
from hmmwriter import HmmWriter
hmm_dir = parameter_dir + '/hmms'
utils.prep_dir(hmm_dir, '*.yaml')
gene_list = self.args.only_genes
if gene_list == None: # if specific genes weren't specified, do the ones for which we have matches
gene_list = []
for region in utils.regions:
for gene in self.germline_seqs[region]:
if sw_matches == None or gene in sw_matches: # shouldn't be None really, but I'm testing something
gene_list.append(gene)
for gene in gene_list:
if self.args.debug:
print ' %s' % utils.color_gene(gene)
writer = HmmWriter(
parameter_dir, hmm_dir, gene, self.args.naivety,
self.germline_seqs[utils.get_region(gene)][gene], self.args)
writer.write()
print ' time to write hmms: %.3f' % (time.time() - start)
def write_vdjalign_input(self, base_infname):
# first make a list of query names so we can iterate over an ordered collection
ordered_info = []
for query_name in self.input_info:
ordered_info.append(query_name)
queries_per_proc = float(len(self.input_info)) / self.args.n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
if self.args.n_procs == 1: # double check for rounding problems or whatnot
assert n_queries_per_proc == len(self.input_info)
for iproc in range(self.args.n_procs):
workdir = self.args.workdir
if self.args.n_procs > 1:
workdir += '/sw-' + str(iproc)
utils.prep_dir(workdir)
infname = workdir + '/' + base_infname
with opener('w')(workdir + '/' + base_infname) as sub_infile:
for iquery in range(iproc * n_queries_per_proc,
(iproc + 1) * n_queries_per_proc):
if iquery >= len(ordered_info):
break
query_name = ordered_info[iquery]
sub_infile.write('>' + str(query_name) + ' NUKES\n')
sub_infile.write(self.input_info[query_name]['seq'] + '\n')
def split_input(self, n_procs, infname=None, info=None, prefix='sub'):
"""
If <infname> is specified split the csv info from it into <n_procs> input files in subdirectories labelled with '<prefix>-' within <self.args.workdir>
If <info> is specified, instead split the list <info> into pieces and return a list of the resulting lists
"""
if info is None:
assert infname is not None
info = []
with opener('r')(infname) as infile:
reader = csv.DictReader(infile)
for line in reader:
info.append(line)
else:
assert infname is None # make sure only *one* of 'em is specified
outlists = []
queries_per_proc = float(len(info)) / n_procs
n_queries_per_proc = int(math.ceil(queries_per_proc))
for iproc in range(n_procs):
if infname is None:
outlists.append([])
else:
subworkdir = self.args.workdir + '/' + prefix + '-' + str(iproc)
utils.prep_dir(subworkdir)
sub_outfile = opener('w')(subworkdir + '/' + os.path.basename(infname))
writer = csv.DictWriter(sub_outfile, reader.fieldnames)
writer.writeheader()
for iquery in range(iproc*n_queries_per_proc, (iproc + 1)*n_queries_per_proc):
if iquery >= len(info):
break
if infname is None:
outlists[-1].append(info[iquery])
else:
writer.writerow(info[iquery])
if infname is None:
return outlists
def plot(self, base_plotdir, cyst_positions=None, tryp_positions=None):
if not self.finalized:
self.finalize()
plotdir = base_plotdir + '/mute-freqs'
utils.prep_dir(plotdir + '/plots', multilings=('*.csv', '*.svg'))
for region in utils.regions:
utils.prep_dir(plotdir + '/' + region + '/plots', multilings=('*.csv', '*.svg'))
utils.prep_dir(plotdir + '/' + region + '-per-base/plots', multilings=('*.csv', '*.png'))
for gene in self.counts:
counts, plotting_info = self.counts[gene], self.plotting_info[gene]
sorted_positions = sorted(counts)
hist = TH1D('hist_' + utils.sanitize_name(gene), '',
sorted_positions[-1] - sorted_positions[0] + 1,
sorted_positions[0] - 0.5, sorted_positions[-1] + 0.5)
for position in sorted_positions:
hist.SetBinContent(hist.FindBin(position), counts[position]['freq'])
hi_diff = abs(counts[position]['freq'] - counts[position]['freq_hi_err'])
lo_diff = abs(counts[position]['freq'] - counts[position]['freq_lo_err'])
err = 0.5*(hi_diff + lo_diff)
hist.SetBinError(hist.FindBin(position), err)
plotfname = plotdir + '/' + utils.get_region(gene) + '/plots/' + utils.sanitize_name(gene) + '.svg'
xline = None
if utils.get_region(gene) == 'v' and cyst_positions is not None:
xline = cyst_positions[gene]['cysteine-position']
elif utils.get_region(gene) == 'j' and tryp_positions is not None:
xline = int(tryp_positions[gene])
plotting.draw(hist, 'int', plotdir=plotdir + '/' + utils.get_region(gene), plotname=utils.sanitize_name(gene), errors=True, write_csv=True, xline=xline, draw_str='e') #, cwidth=4000, cheight=1000)
paramutils.make_mutefreq_plot(plotdir + '/' + utils.get_region(gene) + '-per-base', utils.sanitize_name(gene), plotting_info)
# for region in utils.regions:
# utils.prep_dir(plotdir + '/' + region + '/tmp/plots', multilings=('*.csv', '*.svg'))
# for gene in self.tmpcounts:
# for position in self.tmpcounts[gene]:
# roothist = plotting.make_hist_from_my_hist_class(self.tmpcounts[gene][position]['muted'], gene + '_' + str(position))
# plotting.draw(roothist, 'int', plotdir=plotdir + '/' + utils.get_region(gene) + '/tmp', plotname=utils.sanitize_name(gene) + '_' + str(position), errors=True, write_csv=True) #, cwidth=4000, cheight=1000)
# make mean mute freq hists
hist = plotting.make_hist_from_my_hist_class(self.mean_rates['all'], 'all-mean-freq')
plotting.draw(hist, 'float', plotname='all-mean-freq', plotdir=plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True)
for region in utils.regions:
hist = plotting.make_hist_from_my_hist_class(self.mean_rates[region], region+'-mean-freq')
plotting.draw(hist, 'float', plotname=region+'-mean-freq', plotdir=plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True)
check_call(['./bin/makeHtml', plotdir, '3', 'null', 'svg'])
# then write html file and fix permissiions
for region in utils.regions:
check_call(['./bin/makeHtml', plotdir + '/' + region, '1', 'null', 'svg'])
check_call(['./bin/makeHtml', plotdir + '/' + region + '-per-base', '1', 'null', 'png'])
check_call(['./bin/permissify-www', plotdir]) # NOTE this should really permissify starting a few directories higher up
def plot(self, base_plotdir, cyst_positions=None, tryp_positions=None, only_csv=False):
if not self.finalized:
self.finalize()
plotdir = base_plotdir + '/mute-freqs'
overall_plotdir = plotdir + '/overall'
utils.prep_dir(overall_plotdir, multilings=('*.csv', '*.svg'))
for region in utils.regions:
utils.prep_dir(plotdir + '/' + region, multilings=('*.csv', '*.svg'))
# utils.prep_dir(plotdir + '/' + region + '-per-base/plots', multilings=('*.csv', '*.png'))
if self.tigger:
utils.prep_dir(plotdir + '/tigger', multilings=('*.csv', '*.svg'))
for gene in self.freqs:
freqs = self.freqs[gene]
sorted_positions = sorted(freqs.keys())
genehist = Hist(sorted_positions[-1] - sorted_positions[0] + 1, sorted_positions[0] - 0.5, sorted_positions[-1] + 0.5, xtitle='fixme', ytitle='fixme') #, title=utils.sanitize_name(gene))
for position in sorted_positions:
hi_diff = abs(freqs[position]['freq'] - freqs[position]['freq_hi_err'])
lo_diff = abs(freqs[position]['freq'] - freqs[position]['freq_lo_err'])
err = 0.5*(hi_diff + lo_diff)
genehist.set_ibin(genehist.find_bin(position), freqs[position]['freq'], error=err)
xline = None
figsize = [3, 3]
if utils.get_region(gene) == 'v' and cyst_positions is not None:
xline = cyst_positions[gene]
figsize[0] *= 3.5
elif utils.get_region(gene) == 'j' and tryp_positions is not None:
xline = tryp_positions[gene]
figsize[0] *= 2
plotting.draw_no_root(genehist, plotdir=plotdir + '/' + utils.get_region(gene), plotname=utils.sanitize_name(gene), errors=True, write_csv=True, xline=xline, figsize=figsize, only_csv=only_csv)
# paramutils.make_mutefreq_plot(plotdir + '/' + utils.get_region(gene) + '-per-base', utils.sanitize_name(gene), plotting_info) # needs translation to mpl
# make mean mute freq hists
plotting.draw_no_root(self.mean_rates['all'], plotname='all-mean-freq', plotdir=overall_plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True, only_csv=only_csv)
for region in utils.regions:
plotting.draw_no_root(self.mean_rates[region], plotname=region+'-mean-freq', plotdir=overall_plotdir, stats='mean', bounds=(0.0, 0.4), write_csv=True, only_csv=only_csv)
if self.tigger:
self.tigger_plot(only_csv)
if not only_csv: # write html file and fix permissiions
plotting.make_html(overall_plotdir)
for region in utils.regions:
plotting.make_html(plotdir + '/' + region, n_columns=1)
def __init__(self, args, seed, sublabel=None, total_length_from_right=-1):
self.args = args
if sublabel == None:
self.workdir = self.args.workdir + '/recombinator'
self.outfname = self.args.outfname
else: # need a separate workdir for each subprocess
self.workdir = self.args.workdir + '/recombinator-' + sublabel
self.outfname = self.workdir + '/' + os.path.basename(
self.args.outfname)
utils.prep_dir(self.workdir)
if not os.path.exists(self.args.parameter_dir):
raise Exception('ERROR ' + self.args.parameter_dir + ' d.n.e')
# parameters that control recombination, erosion, and whatnot
self.total_length_from_right = total_length_from_right # measured from right edge of j, only write to file this much of the sequence (our read lengths are 130 by this def'n a.t.m.)
self.all_seqs = {} # all the Vs, all the Ds...
self.index_keys = {
} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.version_freq_table = {
} # list of the probabilities with which each VDJ combo appears in data
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
# first read info that doesn't depend on which person we're looking at
self.all_seqs = utils.read_germlines(self.args.datadir)
with opener('r')(
self.args.datadir + '/v-meta.json'
) as json_file: # get location of <begin> cysteine in each v region
self.cyst_positions = json.load(json_file)
with opener('r')(
self.args.datadir + '/j_tryp.csv'
) as csv_file: # get location of <end> tryptophan in each j region (TGG)
tryp_reader = csv.reader(csv_file)
self.tryp_positions = {
row[0]: row[1]
for row in tryp_reader
} # WARNING: this doesn't filter out the header line
# then read stuff that's specific to each person
self.read_vdj_version_freqs(self.args.parameter_dir + '/' +
utils.get_parameter_fname('all'))
self.read_insertion_content()
if self.args.naivety == 'M': # read shm info if non-naive is requested
# NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with opener('r')(
self.args.gtrfname) as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line[
'value']
treegen = treegenerator.TreeGenerator(args,
self.args.parameter_dir,
seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(seed, self.treefname)
with opener('r')(
self.treefname
) as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
if not self.args.no_clean:
os.remove(self.treefname)
if os.path.exists(self.outfname):
os.remove(self.outfname)
elif not os.path.exists(os.path.dirname(os.path.abspath(
self.outfname))):
os.makedirs(os.path.dirname(os.path.abspath(self.outfname)))
def clean_plots(self, plotdir):
for substr in self.subplotdirs:
utils.prep_dir(plotdir + '/' + substr,
wildlings=('*.csv', '*.svg'))
default='partis')
parser.add_argument('--overwrite', action='store_true')
parser.add_argument('--igbdir', default='./packages/ncbi-igblast-1.6.1/bin')
parser.add_argument('--glfo-dir')
parser.add_argument('--simulation-germline-dir')
parser.add_argument('--locus', default='igh')
parser.add_argument('--region', default='v')
parser.add_argument('--species', default='human')
parser.add_argument('--slurm', action='store_true')
parser.add_argument('--changeo-path', default=os.getenv('HOME') + '/.local')
parser.add_argument('--condapath',
default=os.getenv('HOME') + '/miniconda3/bin')
args = parser.parse_args()
# ----------------------------------------------------------------------------------------
outdir = os.path.dirname(
args.outfname
) # kind of annoying having <args.workdir> and <outdir>, but the former is for stuff we don't want to keep (not much... maybe just .cmd file), and the latter is for stuff we do
assert outdir.split('/')[-1] == args.locus
outdir = outdir.rstrip('/' + args.locus)
utils.prep_dir(args.workdir, wildlings=['*.cmd', '*.fa', '*.sh']) #'*.fmt7'])
utils.prep_dir(outdir, allow_other_files=True)
initialize_germline_info(outdir) # deal with igblast germline crap
outfname = run_alignment(
args, outdir) # get the alignments, either with igblast or partis
run_tigger(outfname, args.outfname, outdir)
os.rmdir(args.workdir)
utils.print_reco_event(annotations[uid])
n_above_cutoff = len(
[_ for cfo in chfo.values() if cfo['max_abs_diff'] > args.cutoff])
chimeric_fraction = n_above_cutoff / float(len(chfo))
print ' %d / %d = %.3f above chimeric cutoff' % (n_above_cutoff, len(chfo),
chimeric_fraction)
hmaxval = Hist(45, 0., 0.65)
for uid in annotations:
hmaxval.fill(chfo[uid]['max_abs_diff'])
himax = Hist(75, 0., 400)
for uid in annotations:
himax.fill(chfo[uid]['imax'])
utils.prep_dir(args.plotdir, wildlings=['*.svg', '*.csv'])
import matplotlib
from matplotlib import pyplot as plt
fig, ax = plotting.mpl_init()
xvals, yvals = zip(*[(v['imax'], v['max_abs_diff']) for v in chfo.values()])
plt.scatter(xvals, yvals, alpha=0.4)
print 'writing to %s' % args.plotdir
plotting.mpl_finish(ax,
args.plotdir,
'hexbin',
title=args.title,
xlabel='break point',
ylabel='abs mfreq diff')
def __init__(
self, args, glfo, seed, workdir, outfname
): # NOTE <gldir> is not in general the same as <args.initial_germline_dir> # rm workdir
self.args = args
self.glfo = glfo
# NOTE in general *not* the same as <self.args.workdir> and <self.args.outfname>
self.workdir = tempfile.mkdtemp()
self.outfname = outfname
utils.prep_dir(self.workdir)
# set <self.parameter_dir> (note that this is in general *not* the same as self.args.parameter_dir)
if self.args.rearrange_from_scratch: # currently not allowed to mutate from scratch without also rearranging from scratch (enforced in bin/partis)
if self.args.mutate_from_scratch:
self.parameter_dir = None
else:
self.parameter_dir = self.args.scratch_mute_freq_dir # if you make up mute freqs from scratch, unless you're really careful you tend to get nonsense results for a lot of things (e.g. allele finding). So it's easier to copy over a reasonable set of mut freq parameters from somewhere.
else:
self.parameter_dir = self.args.parameter_dir + '/' + self.args.parameter_type
self.index_keys = {
} # this is kind of hackey, but I suspect indexing my huge table of freqs with a tuple is better than a dict
self.mute_models = {}
# self.treeinfo = [] # list of newick-formatted tree strings with region-specific branch info tacked at the end
for region in utils.regions:
self.mute_models[region] = {}
for model in ['gtr', 'gamma']:
self.mute_models[region][model] = {}
self.allele_prevalence_freqs = glutils.read_allele_prevalence_freqs(
args.allele_prevalence_fname
) if args.allele_prevalence_fname is not None else {}
self.version_freq_table = self.read_vdj_version_freqs(
) # list of the probabilities with which each VDJ combo (plus other rearrangement parameters) appears in data (none if rearranging from scratch)
self.insertion_content_probs = self.read_insertion_content(
) # dummy/uniform if rearranging from scratch
self.all_mute_freqs = {}
# read shm info NOTE I'm not inferring the gtr parameters a.t.m., so I'm just (very wrongly) using the same ones for all individuals
with open(self.args.gtrfname, 'r') as gtrfile: # read gtr parameters
reader = csv.DictReader(gtrfile)
for line in reader:
parameters = line['parameter'].split('.')
region = parameters[0][3].lower()
assert region == 'v' or region == 'd' or region == 'j'
model = parameters[1].lower()
parameter_name = parameters[2]
assert model in self.mute_models[region]
self.mute_models[region][model][parameter_name] = line['value']
treegen = treegenerator.TreeGenerator(args,
self.parameter_dir,
seed=seed)
self.treefname = self.workdir + '/trees.tre'
treegen.generate_trees(
seed, self.treefname
) # NOTE not really a newick file, since I hack on the per-region branch length info at the end of each line
with open(
self.treefname, 'r'
) as treefile: # read in the trees (and other info) that we just generated
self.treeinfo = treefile.readlines()
os.remove(self.treefname)
if os.path.exists(self.outfname):
os.remove(self.outfname)
elif not os.path.exists(os.path.dirname(os.path.abspath(
self.outfname))):
os.makedirs(os.path.dirname(os.path.abspath(self.outfname)))
self.validation_values = {
'heights':
{t: {
'in': [],
'out': []
}
for t in ['all'] + utils.regions}
}
def get_gls_gen_annotation_performance_plots(args, region, baseoutdir):
assert region == 'v' # needs to be implemented
import plotting
import plotconfig
methcolors = { # NOTE started from scolors in bin/plot-gl-set-trees.py htmlcolorcods.com, and slide each one a little rightward
'tigger-default' : '#dd4d39',
'igdiscover' : '#55ab7a', #60ac84',
'partis' : '#6b83ca', #758bcd',
'full' : '#858585',
}
lstyledict = {} # 'tigger-default' : '--'}
lwdict = {'full' : 9, 'igdiscover' : 8, 'partis' : 5, 'tigger-default' : 2} # methods are sorted below, so it's always [full, igdiscover, partis, tigger]
linewidths = [lwdict[m] for m in args.methods]
colors = [methcolors[meth] for meth in args.methods]
linestyles = [lstyledict.get(m, '-') for m in args.methods]
alphas = [0.8 if m in ['full', 'igdiscover'] else 1 for m in args.methods]
varname = args.action
varval = 'simu'
plotnames = ['v_hamming_to_true_naive', 'v_muted_bases']
xtitles = ['V distance to true naive', 'inferred - true']
meanvals = {pn : {m : [] for m in args.methods} for pn in plotnames}
print ' annotations: %s' % get_outdir(args, baseoutdir, varname, varval, n_events=args.gls_gen_events)
all_hists = {pn : [] for pn in plotnames}
for iproc in range(args.iteststart, args.n_tests):
outdir = get_outdir(args, baseoutdir, varname, varval, n_events=args.gls_gen_events) + '/' + str(iproc) # duplicates code in bin/test-germline-inference.py
plotdir = outdir + '/annotation-performance-plots'
print ' %s' % plotdir
if not args.only_print:
utils.prep_dir(plotdir, wildlings=['*.png', '*.svg', '*.csv'])
# shenanigans for the six (three easy and thre hard) of 'em that go in the paper pdf
make_legend = (iproc > 2) or (iproc == 0) # and args.gls_gen_difficulty == 'easy')
make_xtitle = (iproc > 2) or (iproc == 2)
make_ytitle = (iproc > 2) or (args.gls_gen_difficulty == 'easy')
for plotname in plotnames:
hfnames = {meth : get_gls_fname(region, outdir, meth, args.locus, annotation_performance_plots=True) for meth in args.methods}
for hfn in hfnames.values():
if not os.path.exists(hfn):
raise Exception('%s d.n.e.: need to first run non-plotting (without --plot) --annotation-performance-plots (which involves re-running partis, I think the difference being partis is now running with --plot-annotation-performance' % hfn)
hists = {meth : Hist(fname=hfnames[meth] + '/' + plotname + '.csv', title=methstr(meth) if make_legend else None) for meth in args.methods}
for meth in args.methods:
if hists[meth].overflow_contents() != 0.0:
print ' %s %s non-zero under/overflow %f' % (utils.color('red', 'error'), methstr(meth), hists[meth].overflow_contents())
meanvals[plotname][meth].append(hists[meth].get_mean())
if args.only_print:
continue
plotting.draw_no_root(hists[args.methods[0]], log='y', plotdir=plotdir, plotname=plotname, more_hists=[hists[m] for m in args.methods[1:]], colors=colors, ytitle='sequences' if make_ytitle else None,
xtitle=xtitles[plotnames.index(plotname)] if make_xtitle else '',
plottitle=gls_sim_str(args.gls_gen_difficulty, iproc),
linewidths=linewidths, linestyles=linestyles, alphas=alphas, remove_empty_bins=True, square_bins=True)
all_hists[plotname].append(hists)
print ' total plots'
plotdir = get_outdir(args, baseoutdir, varname, varval, n_events=args.gls_gen_events) + '/annotation-performance-plots'
print ' %s' % plotdir
if not args.only_print:
utils.prep_dir(plotdir, wildlings=['*.png', '*.svg', '*.csv'])
for plotname in plotnames:
total_hists = {}
for meth in args.methods:
xmin = min([hdict[meth].xmin for hdict in all_hists[plotname]])
xmax = max([hdict[meth].xmax for hdict in all_hists[plotname]])
total_hists[meth] = Hist(xmax - xmin, xmin, xmax, title=all_hists[plotname][0][meth].title)
for hdict in all_hists[plotname]:
assert hdict[meth].integral(include_overflows=True) > 100 # make sure it isn't normalized (this is a shitty way to do this)
bin_centers = hdict[meth].get_bin_centers()
for ibin in range(len(hdict[meth].low_edges)):
xval = bin_centers[ibin]
for _ in range(int(hdict[meth].bin_contents[ibin])):
total_hists[meth].fill(xval)
plotting.draw_no_root(total_hists[args.methods[0]], log='y', plotdir=plotdir, plotname='total-' + plotname, more_hists=[total_hists[m] for m in args.methods[1:]], colors=colors, ytitle='sequences' if make_ytitle else None,
xtitle=xtitles[plotnames.index(plotname)],
plottitle=gls_sim_str(args.gls_gen_difficulty, iproc=''),
linewidths=linewidths, linestyles=linestyles, alphas=alphas, remove_empty_bins=True, square_bins=True)
for plotname in plotnames:
if 'muted_bases' in plotname: # mean value isn't meaningful
continue
print plotname
for meth in args.methods:
mean = float(sum(meanvals[plotname][meth])) / len(meanvals[plotname][meth])
err = numpy.std(meanvals[plotname][meth], ddof=1) / math.sqrt(len(meanvals[plotname][meth]))
print ' %15s %6.3f / %d = %6.2f +/- %6.2f' % (methstr(meth), sum(meanvals[plotname][meth]), len(meanvals[plotname][meth]), mean, err)
def clean_plots(self, plotdir):
for substr in ['overall', ] + utils.regions: # + [r + '-per-base' for r in utils.regions]:
utils.prep_dir(plotdir + '/' + substr, wildlings=('*.csv', '*.svg'))
