def test_bad_cell_position_in_slice(self):
electrodeParams = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': 200,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11),
'method': "pointsource",
}
stickParams = {
'morphology': os.path.join(LFPy.__path__[0], 'test', 'stick.hoc'),
'passive_parameters': {'g_pas': 1. / 30000, 'e_pas': -65},
'passive': True,
'tstart': -10,
'tstop': 20,
'dt': 2**-4,
'nsegs_method': 'lambda_f',
'lambda_f': 1000,
}
stick = LFPy.Cell(**stickParams)
stick.set_rotation(y=np.pi / 2)
stick.simulate(rec_imem=True)
stick.set_pos(z=-100)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA.get_transformation_matrix)
stick.set_pos(z=300)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA.get_transformation_matrix)
def test_sqeeze_cell_and_bad_position(self):
electrodeParams = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': 200,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11),
'method': "pointsource",
'squeeze_cell_factor': None,
}
stickParams = {
'morphology': os.path.join(
LFPy.__path__[0],
'test',
'ball_and_sticks.hoc'),
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65},
'passive': True,
'tstart': -10,
'tstop': 20,
'dt': 2**-4,
'nsegs_method': 'lambda_f',
'lambda_f': 1000,
}
stick = LFPy.Cell(**stickParams)
stick.set_rotation(y=np.pi / 2)
stick.simulate(rec_imem=True)
stick.set_pos(z=1)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA._test_cell_extent)
stick.set_pos(z=199)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA._test_cell_extent)
electrodeParams = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': 200,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11),
'method': "pointsource",
'squeeze_cell_factor': 0.1,
}
stick.set_pos(z=-1)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA._test_cell_extent)
stick.set_pos(z=201)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA._test_cell_extent)
def test_position_shifted_slice(self):
electrodeParams = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': 200,
'z_shift': -200,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11) - 100,
'method': "pointsource",
'squeeze_cell_factor': None,
}
stickParams = {
'morphology': os.path.join(
LFPy.__path__[0],
'test',
'ball_and_sticks.hoc'),
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65},
'passive': True,
'tstart': -10,
'tstop': 20,
'dt': 2**-4,
'nsegs_method': 'lambda_f',
'lambda_f': 100,
}
stick = LFPy.Cell(**stickParams)
stick.set_rotation(y=np.pi / 2)
stick.set_pos(z=-100)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
MEA._test_cell_extent()
def test_return_comp_outside_slice(self):
electrodeParams = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': 200,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11),
'method': "pointsource",
'squeeze_cell_factor': None,
}
stickParams = {
'morphology': os.path.join(
LFPy.__path__[0],
'test',
'ball_and_sticks.hoc'),
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65},
'passive': True,
'tstart': -10,
'tstop': 20,
'dt': 2**-4,
'nsegs_method': 'lambda_f',
'lambda_f': 1000,
}
stick = LFPy.Cell(**stickParams)
stick.set_rotation(y=np.pi / 2)
stick.set_pos(z=100)
stick.simulate(rec_imem=True)
MEA = LFPy.RecMEAElectrode(stick, **electrodeParams)
np.testing.assert_raises(RuntimeError, MEA._return_comp_outside_slice)
true_bad_comp = np.array([2, 3, 6])
stick.z[true_bad_comp, 0] = 1000
bad_comp, reason = MEA._return_comp_outside_slice()
np.testing.assert_equal(reason, "zstart above")
np.testing.assert_equal(true_bad_comp, bad_comp)
stick.z[true_bad_comp, 0] = 100
stick.z[true_bad_comp, 0] = -1000
bad_comp, reason = MEA._return_comp_outside_slice()
np.testing.assert_equal(reason, "zstart below")
np.testing.assert_equal(true_bad_comp, bad_comp)
stick.z[true_bad_comp, 0] = 100
stick.z[true_bad_comp, 1] = 1000
bad_comp, reason = MEA._return_comp_outside_slice()
np.testing.assert_equal(reason, "zend above")
np.testing.assert_equal(true_bad_comp, bad_comp)
stick.z[true_bad_comp, 1] = 100
stick.z[true_bad_comp, 1] = -1000
bad_comp, reason = MEA._return_comp_outside_slice()
np.testing.assert_equal(reason, "zend below")
np.testing.assert_equal(true_bad_comp, bad_comp)
stick.z[true_bad_comp, 1] = 100
print(
'Network build finished with {} connections and {} synapses in {} seconds'
.format(total_conncount, total_syncount, create_connections_time))
logfile.write('connections {}\n'.format(create_connections_time))
tic = time()
############################################################################
# Set up extracellular electrode
############################################################################
if PSET.COMPUTE_LFP:
electrode = LFPy.RecExtElectrode(**PSET.electrodeParams)
else:
electrode = None
if PSET.COMPUTE_ECOG:
ecog_electrode = LFPy.RecMEAElectrode(**PSET.ecogParameters)
electrode = [electrode, ecog_electrode]
############################################################################
# Recording of additional variables
############################################################################
if RANK == 0:
network.t = neuron.h.Vector()
network.t.record(neuron.h._ref_t)
else:
network.t = None
############################################################################
# run simulation, gather results across all RANKs
############################################################################
# Assert that connect routines has finished across RANKS before starting
def test_slice_shift_invariance_pointsource(self):
h = 200
z_shift_1 = 0
z_shift_2 = -352
electrodeParams_1 = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': h,
'z_shift': z_shift_1,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11) + z_shift_1,
'squeeze_cell_factor': None,
}
electrodeParams_2 = {
'sigma_T': 0.3,
'sigma_S': 1.5,
'sigma_G': 0.0,
'h': h,
'z_shift': z_shift_2,
'x': np.linspace(0, 1000, 11),
'y': np.zeros(11),
'z': np.zeros(11) + z_shift_2,
'squeeze_cell_factor': None,
}
stimParams = {
'pptype': 'SinSyn',
'delay': -100.,
'dur': 1000.,
'pkamp': 1.,
'freq': 100.,
'phase': -np.pi / 2,
'bias': 0.,
'record_current': True
}
stickParams = {
'morphology': os.path.join(
LFPy.__path__[0],
'test',
'ball_and_sticks.hoc'),
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65},
'passive': True,
'tstart': -10,
'tstop': 20,
'dt': 2**-4,
'nsegs_method': 'lambda_f',
'lambda_f': 100,
}
stick = LFPy.Cell(**stickParams)
stick.set_rotation(y=np.pi / 2)
LFPy.StimIntElectrode(stick, stick.get_closest_idx(0, 0, 0),
**stimParams)
stick.simulate(rec_imem=True)
methods = ["pointsource", "linesource", "root_as_point"]
for method in methods:
electrodeParams_1["method"] = method
electrodeParams_2["method"] = method
stick.set_pos(z=z_shift_1 + h / 2)
MEA_shift_1 = LFPy.RecMEAElectrode(stick, **electrodeParams_1)
M_1 = MEA_shift_1.get_transformation_matrix()
stick.set_pos(z=z_shift_2 + h / 2)
MEA_shift_2 = LFPy.RecMEAElectrode(stick, **electrodeParams_2)
M_2 = MEA_shift_2.get_transformation_matrix()
np.testing.assert_allclose(M_1 @ stick.imem,
M_2 @ stick.imem, rtol=1E-7)
[top_of_cortex] *
num_ECoG_elecs), # Assume that top of cortex is 50 um above cell top
'n': 200,
'r': 500, # ECoG radii are often 500-1000 um
'N': N,
'method': "pointsource",
}
#perform NEURON simulation, results saved as attributes in the cell instance
cell.simulate(rec_imem=True)
# Initialize electrode geometry, then calculate the LFP, using the
# electrode classes. Note that now cell is given as input to electrode
# and created after the NEURON simulations are finished
electrode = LFPy.RecExtElectrode(cell, **electrodeParameters)
electrode.calc_lfp()
electrode.LFP -= np.average(electrode.LFP, axis=1)[:, None]
electrode_MoI = LFPy.RecMEAElectrode(cell, **elec_with_MoIParameters)
electrode_MoI.calc_lfp()
electrode_MoI.LFP -= np.average(electrode_MoI.LFP, axis=1)[:, None]
ecog_electrode = LFPy.RecMEAElectrode(cell, **ecogParameters)
ecog_electrode.calc_lfp()
ecog_electrode.LFP -= np.average(ecog_electrode.LFP, axis=1)[:, None]
#plotting some variables and geometry, saving output to .pdf.
fig = plot_LFP_and_ECoG(cell, electrode, electrode_MoI, ecog_electrode)
fig.savefig('example_ECoG.pdf', dpi=300)
plt.show()
MEA_electrode_parameters = {
'sigma_T' : 0.3, # extracellular conductivity
'sigma_G' : 0.0, # MEA glass electrode plate conductivity
'sigma_S' : 1.5, # Saline bath conductivity
'x' : X.flatten(), # electrode requires 1d vector of positions
'y' : Y.flatten(),
'z' : Z.flatten(),
"method": "soma_as_point",
'N' : np.array([[0, 0, 1]]*X.size), #surface normals
'r' : 50, # contact site radius
'n' : 100, # datapoints for averaging,
"seedvalue": 12,
"squeeze_cell_factor": 0.4,
}
# Run simulation, electrode object argument in cell.simulate
print("running simulation...")
cell.simulate(rec_imem=True, rec_vmem=True)
# Create electrode objects
electrode = LFPy.RecExtElectrode(cell, **grid_electrode_parameters)
MEA = LFPy.RecMEAElectrode(cell, **MEA_electrode_parameters)
# Calculate LFPs
MEA.calc_lfp()
electrode.calc_lfp()
plot_results(cell, synapse, MEA, electrode)
plt.show()
'{} connections and {} synapses in {} seconds'.format(
total_conncount, total_syncount, create_connections_time))
logfile.write('connections {}\n'.format(create_connections_time))
tic = time()
##########################################################################
# Set up extracellular electrodes and devices
##########################################################################
probes = []
if PSET.COMPUTE_LFP:
electrode = LFPy.RecExtElectrode(cell=None, **PSET.electrodeParams)
probes.append(electrode)
if PSET.COMPUTE_ECOG:
ecog_electrode = LFPy.RecMEAElectrode(cell=None, **PSET.ecogParameters)
probes.append(ecog_electrode)
if PSET.COMPUTE_P:
current_dipole_moment = LFPy.CurrentDipoleMoment(cell=None)
probes.append(current_dipole_moment)
##########################################################################
# Recording of additional variables
##########################################################################
if RANK == 0:
network.t = neuron.h.Vector()
network.t.record(neuron.h._ref_t)
else:
network.t = None
elec_z_mea = np.array([0, 0])
sigma = 1 # S/m
noise_level = 10 # uV
# Define electrode parameters
mea_parameters = {
'sigma_T': sigma, # Saline bath conductivity
'sigma_S': sigma, # Saline bath conductivity
'sigma_G': 0, # Saline bath conductivity
'x': elec_x_mea, # electrode requires 1d vector of positions
'y': elec_y_mea,
'z': elec_z_mea,
"method": "soma_as_point",
}
mea_electrode = LFPy.RecMEAElectrode(cell, **mea_parameters)
mea_electrode.calc_lfp()
# mea_electrode.LFP *= 2 # Because of non-conducting electrode plane
soma_diam = 10
elec_x = np.linspace(-200 + soma_diam / 2, -100 + soma_diam / 2, 50)
elec_y = np.zeros(len(elec_x))
elec_z = np.ones(len(elec_x)) * 65
# Define electrode parameters
elec_parameters = {
'sigma': sigma, # Saline bath conductivity
'x': elec_x, # electrode requires 1d vector of positions
'y': elec_y,
'z': elec_z,