def main():
parser = optparse.OptionParser(version="%prog version: $Id$", usage=USAGE)
parser.add_option(
"--method",
dest="method",
type="choice",
choices=("view", "align", "pileup", "profile"),
help="method to perform [default=%default].",
)
parser.add_option(
"--mode", dest="mode", type="choice", choices=("global", "local"), help="alignment mode [default=%default]."
)
parser.add_option("--gop", dest="gop", type="float", help="gap opening penalty [default=%default].")
parser.add_option("--gep", dest="gep", type="float", help="gap extension penalty [default=%default].")
parser.set_defaults(
filename_graph="adda.graph",
filename_index="adda.graph.idx",
method="view",
filename_fasta="adda",
filename_config="adda.ini",
append=False,
force=False,
mode="local",
gop=-10.0,
gep=-1.0,
)
(options, args) = E.Start(parser)
config = AddaIO.ConfigParser()
config.read(os.path.expanduser(options.filename_config))
index = cadda.IndexedNeighbours(options.filename_graph, options.filename_index)
alignlib.getDefaultToolkit().setEncoder(alignlib.getEncoder(alignlib.Protein20))
alignlib.getDefaultToolkit().setRegularizor(alignlib.makeRegularizorDirichletPrecomputed())
alignlib.getDefaultToolkit().setLogOddor(alignlib.makeLogOddorDirichlet(0.3))
alignlib.getDefaultToolkit().setWeightor(alignlib.makeWeightor())
fasta = IndexedFasta.IndexedFasta(options.filename_fasta)
align = AddaProfiles.AddaProfiles(config, fasta=fasta)
if options.method == "view":
for nid in args:
nid = int(args[0])
neighbours = index.getNeighbours(nid)
for n in neighbours:
print str(n)
elif options.method == "pileup":
if "_" in args[0]:
nid, start, end = AddaIO.toTuple(args[0])
else:
nid = int(args[0])
start, end = None, None
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
options.stdout.write("%s\n" % str(mali))
elif options.method == "profile":
if "_" in args[0]:
nid, start, end = AddaIO.toTuple(args[0])
else:
nid = int(args[0])
start, end = None, None
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
prof = alignlib.makeProfile(mali)
E.info("nid: %i, neighours=%i" % (nid, len(neighbours)))
if start != None:
prof.useSegment(start, end)
prof.prepare()
options.stdout.write("%s\n" % str(prof))
elif options.method == "align":
nid1, start1, end1 = AddaIO.toTuple(args[0])
nid2, start2, end2 = AddaIO.toTuple(args[1])
align = AddaProfiles.AddaProfiles(config, fasta=fasta)
if options.mode == "local":
mode = alignlib.ALIGNMENT_LOCAL
else:
mode = alignlib.ALIGNMENT_GLOBAL
alignator = alignlib.makeAlignatorDPFull(mode, options.gop, options.gep)
def _buildProfile(nid, start, end):
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
prof = alignlib.makeProfile(mali)
E.info("nid: %i, neighours=%i" % (nid, len(neighbours)))
prof.useSegment(start, end)
prof.prepare()
seq = fasta.getSequence(nid)
return alignlib.makeSequence(seq), prof
seq1, prof1 = _buildProfile(nid1, start1, end1)
seq2, prof2 = _buildProfile(nid2, start2, end2)
result = alignlib.makeAlignmentVector()
alignator.align(result, prof1, prof2)
E.debug("%s\n" % str(result))
options.stdout.write(
"%s vs %s: score=%5.2f, length=%i, numgaps=%i, row_from=%i, row_to=%i, col_from=%i, col_to=%i\n"
% (
nid1,
nid2,
result.getScore(),
result.getLength(),
result.getNumGaps(),
result.getRowFrom(),
result.getRowTo(),
result.getColFrom(),
result.getColTo(),
)
)
f = alignlib.AlignmentFormatExplicit(result, seq1, seq2)
options.stdout.write("%s\n" % str(f))
E.Stop()
def main():
parser = optparse.OptionParser(version="%prog version: $Id$", usage=USAGE)
parser.add_option("--method",
dest="method",
type="choice",
choices=("view", "align", "pileup", "profile"),
help="method to perform [default=%default].")
parser.add_option("--mode",
dest="mode",
type="choice",
choices=("global", "local"),
help="alignment mode [default=%default].")
parser.add_option("--gop",
dest="gop",
type="float",
help="gap opening penalty [default=%default].")
parser.add_option("--gep",
dest="gep",
type="float",
help="gap extension penalty [default=%default].")
parser.set_defaults(
filename_graph="adda.graph",
filename_index="adda.graph.idx",
method="view",
filename_fasta="adda",
filename_config="adda.ini",
append=False,
force=False,
mode="local",
gop=-10.0,
gep=-1.0,
)
(options, args) = E.Start(parser)
config = AddaIO.ConfigParser()
config.read(os.path.expanduser(options.filename_config))
index = cadda.IndexedNeighbours(options.filename_graph,
options.filename_index)
alignlib.getDefaultToolkit().setEncoder(
alignlib.getEncoder(alignlib.Protein20))
alignlib.getDefaultToolkit().setRegularizor(
alignlib.makeRegularizorDirichletPrecomputed())
alignlib.getDefaultToolkit().setLogOddor(
alignlib.makeLogOddorDirichlet(0.3))
alignlib.getDefaultToolkit().setWeightor(alignlib.makeWeightor())
fasta = IndexedFasta.IndexedFasta(options.filename_fasta)
align = AddaProfiles.AddaProfiles(config, fasta=fasta)
if options.method == "view":
for nid in args:
nid = int(args[0])
neighbours = index.getNeighbours(nid)
for n in neighbours:
print str(n)
elif options.method == "pileup":
if "_" in args[0]:
nid, start, end = AddaIO.toTuple(args[0])
else:
nid = int(args[0])
start, end = None, None
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
options.stdout.write("%s\n" % str(mali))
elif options.method == "profile":
if "_" in args[0]:
nid, start, end = AddaIO.toTuple(args[0])
else:
nid = int(args[0])
start, end = None, None
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
prof = alignlib.makeProfile(mali)
E.info("nid: %i, neighours=%i" % (nid, len(neighbours)))
if start != None:
prof.useSegment(start, end)
prof.prepare()
options.stdout.write("%s\n" % str(prof))
elif options.method == "align":
nid1, start1, end1 = AddaIO.toTuple(args[0])
nid2, start2, end2 = AddaIO.toTuple(args[1])
align = AddaProfiles.AddaProfiles(config, fasta=fasta)
if options.mode == "local":
mode = alignlib.ALIGNMENT_LOCAL
else:
mode = alignlib.ALIGNMENT_GLOBAL
alignator = alignlib.makeAlignatorDPFull(mode, options.gop,
options.gep)
def _buildProfile(nid, start, end):
neighbours = index.getNeighbours(nid)
mali = align.buildMali(nid, neighbours)
prof = alignlib.makeProfile(mali)
E.info("nid: %i, neighours=%i" % (nid, len(neighbours)))
prof.useSegment(start, end)
prof.prepare()
seq = fasta.getSequence(nid)
return alignlib.makeSequence(seq), prof
seq1, prof1 = _buildProfile(nid1, start1, end1)
seq2, prof2 = _buildProfile(nid2, start2, end2)
result = alignlib.makeAlignmentVector()
alignator.align(result, prof1, prof2)
E.debug("%s\n" % str(result))
options.stdout.write( "%s vs %s: score=%5.2f, length=%i, numgaps=%i, row_from=%i, row_to=%i, col_from=%i, col_to=%i\n" %\
(nid1, nid2,
result.getScore(),
result.getLength(),
result.getNumGaps(),
result.getRowFrom(), result.getRowTo(),
result.getColFrom(), result.getColTo()))
f = alignlib.AlignmentFormatExplicit(result, seq1, seq2)
options.stdout.write("%s\n" % str(f))
E.Stop()
