def Stack_Sectioning(self,start,stop,step=0.2, verbose=True):
no = int((stop-start)/step)+1
pos = start
xs = self.ccd.image_size[0]
ys = self.ccd.image_size[1]
# psz = qx.getordernum()
psz = 3
self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16)
self.ccd.SetShutterMode(1)
q = self.ccd.Acquire()
self.pol.MoveAbs(0)
time.sleep(0.4)
for p in range(no):
self.zst.setPositionf(pos)
for m in range(psz):
qx.selecteorder(15+m)
qx.activate()
time.sleep(0.02)
daq.CCDTrig_run(self.handleA,self.handleB)
q = self.ccd.WaitForNewData()
print (p,q)
self.data[psz*p + m] = self.ccd.images
qx.deactivate()
time.sleep(0.02)
pos += step
self.ccd.AbortAcquisition()
self.ccd.SetShutterMode(2)
if verbose:
T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max())
cur_pos = self.zst.getPosition()
self.stackTags(cur_pos,start,stop,step,function='Z-Stack patterns')
return True
def StackExt(self,start,stop,step=0.2,verbose=True):
init_loc=self.zst.getPosition()
no = int((stop-start)/step)+1
pos = start
xs = self.ccd.image_size[0]
ys = self.ccd.image_size[1]
self.data = N.zeros((no,xs,ys), N.uint16)
self.ccd.SetShutterMode(1)
q = self.ccd.Acquire()
time.sleep(0.2) # was 0.05
for p in range(no):
self.zst.setPositionf(pos)
daq.CCDTrig_run(self.handleA,self.handleB)
q = self.ccd.WaitForNewData()
print(p,q)
self.data[p] = self.ccd.images
pos += step
time.sleep(self.delay)
self.ccd.AbortAcquisition()
self.ccd.SetShutterMode(2)
if verbose:
T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max())
cur_pos = self.prior.getPosition()
self.stackTags(cur_pos[0],cur_pos[1],start,stop,step,function='Z-Stack')
self.zst.setPositionf(init_loc)
return True
def singleSnapExt(self,verbose=True):
xs = self.ccd.image_size[0]
ys = self.ccd.image_size[1]
self.data = N.zeros((xs,ys), dtype=N.uint16)
self.ccd.SetShutterMode(1)
self.ccd.Acquire()
time.sleep(0.2) # was 0.05
daq.CCDTrig_run(self.handleA,self.handleB)
self.ccd.WaitForNewData()
self.data[:,:] = self.ccd.images
self.ccd.AbortAcquisition()
self.ccd.SetShutterMode(2)
if verbose:
T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max())
cur_pos = self.prior.getPosition()
self.stackTags(cur_pos[0],cur_pos[1],self.zst.getPosition(),0,0,function='Single snap')
return True
def si_2d_pattern(self,start,stop,step=0.2, verbose=True):
rots = self.pol_array
no = int((stop-start)/step)+1
pos = start
xs = self.ccd.image_size[0]
ys = self.ccd.image_size[1]
# psz = qx.getordernum()
psz = 9
phs = int(psz/3)
self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16)
# self.slm.SLM_on()
self.ccd.SetShutterMode(1)
q = self.ccd.Acquire()
time.sleep(0.1) # was 0.2, changed 20141114
for p in range(no):
self.zst.setPositionf(pos)
for w in range(3):
self.pol.MoveAbs(rots[w])
time.sleep(0.8)
for m in range(phs):
#self.pr.setVoltage(rots[m])
qx.selecteorder(19+phs*w+m)
qx.activate()
time.sleep(0.02)
#print self.pr.getVoltage()
#self.dmd.set_image(patt)
#self.slm.show_next_patt()
#self.slm.show_patt(m)
daq.CCDTrig_run(self.handleA,self.handleB)
q = self.ccd.WaitForNewData()
print (p,q)
self.data[psz*p + 5*w + m] = self.ccd.images
qx.deactivate()
# time.sleep(0.02)
pos += step
self.ccd.AbortAcquisition()
self.ccd.SetShutterMode(2)
# self.slm.SLM_off()
#self.pr.setVoltage(0.0)
if verbose:
T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max())
cur_pos = self.zst.getPosition()
self.stackTags(cur_pos,start,stop,step,function='Z-Stack patterns')
return True
def Image_Patterns(self, angle=0, no=200, pol=0, verbose=True):
pos = self.zst.getPosition()
xs = self.ccd.image_size[0]
ys = self.ccd.image_size[1]
# psz = self.slm.ni
psz = 5
self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16)
# self.slm.SLM_on()
self.ccd.SetShutterMode(1)
q = self.ccd.Acquire()
time.sleep(0.01) # was 0.2, changed 20141114
self.zst.setPositionf(pos)
for p in range(no):
for m in range(angle*5,psz+angle*5):
#self.pr.setVoltage(rots[m])
self.pol.MoveAbs(pol)
qx.selecteorder(m)
qx.activate()
#time.sleep(.50)
#print self.pr.getVoltage()
#self.dmd.set_image(patt)
#self.slm.show_next_patt()
# self.slm.show_patt(m)
daq.CCDTrig_run(self.handleA,self.handleB)
q = self.ccd.WaitForNewData()
print (p,q)
self.data[psz*p+m%5] = self.ccd.images
qx.deactivate()
time.sleep(self.delay)
self.ccd.AbortAcquisition()
self.ccd.SetShutterMode(2)
# self.slm.SLM_off()
#self.pr.setVoltage(0.0)
if verbose:
T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max())
cur_pos = self.prior.getPosition()
self.stackTags(cur_pos[0],cur_pos[1],function='Z-Stack patterns')
return True
def get_img(self):
daq.CCDTrig_run(self.handleA,self.handleB)
self.ccd.WaitForNewData()
self.data[:,:] = self.ccd.images