コード例 #1
0
	def __init__(self, *args, **kwargs):
		super().__init__(*args, **kwargs)

		# load objects
		if SIMULATION is True:
			self.camera = camera.Camera_Simulation()
			self.microcontroller = microcontroller.Microcontroller_Simulation()
		else:
			self.camera = camera.Camera(sn=17910085)
			self.microcontroller = microcontroller.Microcontroller()
		
		self.streamHandler = core.StreamHandler()
		self.liveController = core.LiveController(self.camera,self.microcontroller)
		self.navigationController = core.NavigationController(self.microcontroller)
		#self.autofocusController = core.AutoFocusController(self.camera,self.navigationController,self.liveController)
		#self.multipointController = core.MultiPointController(self.camera,self.navigationController,self.liveController,self.autofocusController)
		self.trackingController = core_tracking.TrackingController(self.microcontroller,self.navigationController)
		self.imageSaver = core.ImageSaver()
		self.imageDisplay = core.ImageDisplay()

		'''
		# thread
		self.thread_multiPoint = QThread()
		self.thread_multiPoint.start()
		self.multipointController.moveToThread(self.thread_multiPoint)
		'''

		# open the camera
		# camera start streaming
		self.camera.open()
		self.camera.set_software_triggered_acquisition() #self.camera.set_continuous_acquisition()
		self.camera.set_callback(self.streamHandler.on_new_frame)
		self.camera.enable_callback()

		# load widgets
		self.cameraSettingWidget = widgets.CameraSettingsWidget(self.camera,self.liveController)
		self.liveControlWidget = widgets.LiveControlWidget(self.streamHandler,self.liveController)
		self.navigationWidget = widgets.NavigationWidget(self.navigationController)
		#self.autofocusWidget = widgets.AutoFocusWidget(self.autofocusController)
		self.recordingControlWidget = widgets.RecordingWidget(self.streamHandler,self.imageSaver)
		self.trackingControlWidget = widgets_tracking.TrackingControllerWidget(self.streamHandler,self.trackingController)
		#self.multiPointWidget = widgets.MultiPointWidget(self.multipointController)

		self.recordTabWidget = QTabWidget()
		self.recordTabWidget.addTab(self.recordingControlWidget, "Simple Recording")
		self.recordTabWidget.addTab(self.trackingControlWidget, "Tracking")
		#self.recordTabWidget.addTab(self.multiPointWidget, "Multipoint Acquisition")

		# layout widgets
		layout = QGridLayout() #layout = QStackedLayout()
		layout.addWidget(self.cameraSettingWidget,0,0)
		layout.addWidget(self.liveControlWidget,1,0)
		layout.addWidget(self.navigationWidget,2,0)
		#layout.addWidget(self.autofocusWidget,3,0)
		layout.addWidget(self.recordTabWidget,4,0)
		
		# transfer the layout to the central widget
		self.centralWidget = QWidget()
		self.centralWidget.setLayout(layout)
		self.setCentralWidget(self.centralWidget)

		# load window
		self.imageDisplayWindow = core.ImageDisplayWindow('Main Display')
		self.imageDisplayWindow.show()

		self.imageDisplayWindow_ThresholdedImage = core.ImageDisplayWindow('Thresholded Image')
		# self.imageDisplayWindow_ThresholdedImage.show()

		# make connections
		self.streamHandler.signal_new_frame_received.connect(self.liveController.on_new_frame)
		self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
		# self.streamHandler.image_to_display.connect(self.imageDisplay.emit_directly) # test emitting image to display without queueing and threading
		self.streamHandler.packet_image_to_write.connect(self.imageSaver.enqueue)
		self.streamHandler.packet_image_for_tracking.connect(self.trackingController.on_new_frame)
		self.imageDisplay.image_to_display.connect(self.imageDisplayWindow.display_image) # may connect streamHandler directly to imageDisplayWindow
		self.navigationController.xPos.connect(self.navigationWidget.label_Xpos.setNum)
		self.navigationController.yPos.connect(self.navigationWidget.label_Ypos.setNum)
		self.navigationController.zPos.connect(self.navigationWidget.label_Zpos.setNum)
		#self.autofocusController.image_to_display.connect(self.imageDisplayWindow.display_image)
		#self.multipointController.image_to_display.connect(self.imageDisplayWindow.display_image)

		self.camera.start_streaming()
コード例 #2
0
    def __init__(self, *args, **kwargs):
        super().__init__(*args, **kwargs)

        # load objects
        self.camera = camera.Camera(sn='39810456',
                                    width=3072,
                                    height=2048,
                                    framerate=60,
                                    color=True)
        self.microcontroller = microcontroller.Microcontroller_Simulation()

        self.configurationManager = core.ConfigurationManager()
        self.streamHandler = core.StreamHandler()
        self.liveController = core.LiveController(self.camera,
                                                  self.microcontroller,
                                                  self.configurationManager)
        self.imageSaver = core.ImageSaver()
        self.imageDisplay = core.ImageDisplay()

        # open the camera
        # camera start streaming
        self.camera.open()
        self.camera.set_software_triggered_acquisition()
        self.camera.set_callback(self.streamHandler.on_new_frame)
        self.camera.enable_callback()

        # load widgets
        self.cameraSettingWidget = widgets.CameraSettingsWidget(
            self.camera, self.liveController)
        self.liveControlWidget = widgets.LiveControlWidget(
            self.streamHandler, self.liveController, self.configurationManager)
        self.recordingControlWidget = widgets.RecordingWidget(
            self.streamHandler, self.imageSaver)

        # layout widgets
        layout = QGridLayout()  #layout = QStackedLayout()
        layout.addWidget(self.cameraSettingWidget, 0, 0)
        layout.addWidget(self.liveControlWidget, 1, 0)
        layout.addWidget(self.recordingControlWidget, 4, 0)

        # transfer the layout to the central widget
        self.centralWidget = QWidget()
        self.centralWidget.setLayout(layout)
        self.setCentralWidget(self.centralWidget)

        # load window
        self.imageDisplayWindow = core.ImageDisplayWindow()
        self.imageDisplayWindow.show()

        # make connections
        self.streamHandler.signal_new_frame_received.connect(
            self.liveController.on_new_frame)
        self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
        self.streamHandler.packet_image_to_write.connect(
            self.imageSaver.enqueue)
        self.imageDisplay.image_to_display.connect(
            self.imageDisplayWindow.display_image
        )  # may connect streamHandler directly to imageDisplayWindow
        self.liveControlWidget.signal_newExposureTime.connect(
            self.cameraSettingWidget.set_exposure_time)
        self.liveControlWidget.signal_newAnalogGain.connect(
            self.cameraSettingWidget.set_analog_gain)
        self.liveControlWidget.update_camera_settings()
コード例 #3
0
    def __init__(self, simulation=False, *args, **kwargs):
        super().__init__(*args, **kwargs)

        self.setWindowTitle('Gravity Machine v3.0')
        self.imaging_channels = CAMERAS.keys()
        print('Available imaging channels: {}'.format(self.imaging_channels))

        #------------------------------------------------------------------
        # Image Displays
        #------------------------------------------------------------------
        self.imageDisplayWindow = {}
        for key in self.imaging_channels:
            self.imageDisplayWindow[key] = core.ImageDisplayWindow(
                key + ' Display', DrawCrossHairs=True)
            if key == TRACKING:
                self.imageDisplayWindow[key].toggle_ROI_selector(True)
        self.imageDisplayWindow_ThresholdedImage = core.ImageDisplayWindow(
            'Thresholded Image')

        #------------------------------------------------------------------
        # Load objects
        #------------------------------------------------------------------
        self.internal_state = core_tracking.InternalState()

        if simulation is True:
            # Define a camera object for each unique image-stream.
            self.camera = {
                key: camera_Daheng.Camera_Simulation()
                for key in self.imaging_channels
            }
            self.microcontroller = microcontroller.Microcontroller_Simulation()
        else:
            self.camera = {}
            for key in self.imaging_channels:
                if (CAMERAS[key]['make'] == 'TIS'):
                    import control.camera_TIS as camera_TIS
                    self.camera[key] = camera_TIS.Camera(
                        serial=CAMERAS[key]['serial'],
                        width=CAMERAS[key]['px_format'][0],
                        height=CAMERAS[key]['px_format'][1],
                        framerate=CAMERAS[key]['fps'],
                        color=CAMERAS[key]['is_color'])
                elif (CAMERAS[key]['make'] == 'Daheng'):
                    self.camera[key] = camera_Daheng.Camera(
                        sn=CAMERAS[key]['serial'])
                self.camera[key].open()
            self.microcontroller = microcontroller.Microcontroller()

        self.microcontroller.configure_actuators()

        self.streamHandler = {}
        self.imageSaver = {}
        for key in self.imaging_channels:
            # load stream handler
            if 'is_polarization' in CAMERAS[key]:
                if CAMERAS[key]['is_polarization']:
                    is_polarization = True
                else:
                    is_polarization = False
            else:
                is_polarization = False
            self.streamHandler[key] = core.StreamHandler(
                camera=self.camera[key],
                crop_width=CAMERAS[key]['px_format'][0],
                crop_height=CAMERAS[key]['px_format'][1],
                imaging_channel=key,
                rotate_image_angle=CAMERAS[key]['rotate image angle'],
                flip_image=CAMERAS[key]['flip image'],
                is_polarization_camera=is_polarization)
            # load image saver
            self.imageSaver[key] = core_tracking.ImageSaver(
                self.internal_state,
                imaging_channel=key,
                image_format=IMAGE_FORMAT)

        self.liveController = {
            key: core.LiveController(self.camera[key], self.microcontroller)
            for key in self.imaging_channels
        }
        self.navigationController = core.NavigationController(
            self.microcontroller)
        self.stateUpdater = core_tracking.StateUpdater(
            self.navigationController, self.internal_state)
        self.microcontroller.set_callback(
            self.stateUpdater.read_microcontroller)
        self.trackingController = core_tracking.TrackingController(
            self.navigationController, self.microcontroller,
            self.internal_state)
        self.trackingDataSaver = core_tracking.TrackingDataSaver(
            self.internal_state)

        #------------------------------------------------------------------
        # load widgets
        #------------------------------------------------------------------
        self.cameraSettingsWidget = {
            key: widgets.CameraSettingsWidget(self.camera[key],
                                              self.liveController[key])
            for key in self.imaging_channels
        }
        self.liveControlWidget = widgets.LiveControlWidget(
            self.streamHandler[TRACKING], self.liveController,
            self.internal_state)
        self.navigationWidget = widgets_tracking.NavigationWidget(
            self.navigationController, self.internal_state)
        self.trackingControlWidget = widgets_tracking.TrackingControllerWidget(
            self.streamHandler[TRACKING], self.trackingController,
            self.trackingDataSaver, self.internal_state,
            self.imageDisplayWindow[TRACKING], self.microcontroller)
        self.PID_Group_Widget = widgets_tracking.PID_Group_Widget(
            self.trackingController)
        self.recordingControlWidget = widgets.RecordingWidget(
            self.streamHandler, self.imageSaver, self.internal_state,
            self.trackingDataSaver, self.imaging_channels)
        self.plotWidget = widgets.dockAreaPlot(self.internal_state)
        self.ledMatrixControlWidget = widgets.LEDMatrixControlWidget(
            self.microcontroller)

        self.liveSettings_Tab = QTabWidget()
        self.liveSettings_Tab.addTab(self.liveControlWidget, 'Live controller')
        for key in self.imaging_channels:
            self.liveSettings_Tab.addTab(self.cameraSettingsWidget[key], key)

        #------------------------------------------------------------------
        # Connections
        #------------------------------------------------------------------
        # Connections that involve all image streams
        for channel in self.imaging_channels:
            self.streamHandler[channel].signal_new_frame_received.connect(
                self.liveController[channel].on_new_frame)
            self.streamHandler[channel].image_to_display.connect(
                self.imageDisplayWindow[channel].display_image)
            self.streamHandler[channel].packet_image_to_write.connect(
                self.imageSaver[channel].enqueue)
            self.imageSaver[channel].imageName.connect(
                self.trackingDataSaver.setImageName)
            self.streamHandler[channel].signal_fps_save.connect(
                self.recordingControlWidget.update_save_fps)

        # Connections that involve only the tracking image stream
        self.streamHandler[TRACKING].thresh_image_to_display.connect(
            self.imageDisplayWindow_ThresholdedImage.display_image)
        self.streamHandler[TRACKING].packet_image_for_tracking.connect(
            self.trackingController.on_new_frame)
        # @@@ Currently the resolution-scaling only controls the TRACKING stream
        self.streamHandler[TRACKING].signal_working_resolution.connect(
            self.liveControlWidget.update_working_resolution)
        # Only display the image-display rate of the main/tracking image stream
        self.streamHandler[TRACKING].signal_fps_display.connect(
            self.liveControlWidget.update_display_fps)
        # self.trackingController.centroid_image.connect(self.imageDisplayWindow[TRACKING].draw_circle)
        self.trackingController.Rect_pt1_pt2.connect(
            self.imageDisplayWindow[TRACKING].draw_rectangle)
        self.trackingController.save_data_signal.connect(
            self.trackingDataSaver.enqueue)
        self.trackingController.signal_tracking_fps.connect(
            self.liveControlWidget.update_stream_fps)
        self.trackingController.signal_update_plots.connect(
            self.plotWidget.update_plots)

        for channel in self.imaging_channels:
            self.streamHandler[channel].signal_fps.connect(
                self.cameraSettingsWidget[channel].update_stream_fps)
        self.trackingController.get_roi_bbox.connect(
            self.imageDisplayWindow[TRACKING].send_bbox)
        self.imageDisplayWindow[TRACKING].roi_bbox.connect(
            self.trackingController.tracker_image.set_roi_bbox)
        self.trackingControlWidget.show_roi.connect(
            self.imageDisplayWindow[TRACKING].toggle_ROI_selector)
        self.recordingControlWidget.start_tracking_signal.connect(
            self.trackingControlWidget.slot_start_tracking)
        self.stateUpdater.signal_joystick_button_pressed.connect(
            self.trackingControlWidget.slot_joystick_button_pressed)
        self.stateUpdater.signal_stage_tracking_status_changed.connect(
            self.trackingControlWidget.slot_update_stage_tracking_status)
        self.liveControlWidget.signal_update_pixel_size.connect(
            self.trackingController.update_pixel_size)
        self.liveControlWidget._update_pixel_size()
        self.liveControlWidget.signal_update_image_resizing_factor.connect(
            self.trackingController.update_image_resizing_factor)
        self.liveControlWidget._update_image_resizing_factor()

        # tracking start/stop
        self.trackingController.signal_stop_tracking.connect(
            self.trackingControlWidget.slot_stop_tracking)
        self.trackingController.signal_stop_tracking.connect(
            self.streamHandler[TRACKING].stop_tracking)

        #-----------------------------------------------------
        # Dock area for image display
        #-----------------------------------------------------
        self.image_window = QMainWindow()
        image_display_dockArea = dock.DockArea()
        self.image_window.setCentralWidget(image_display_dockArea)
        self.image_window.setWindowTitle('Image display')
        image_window_docks = dict()

        last_channel = None
        for channel in self.imaging_channels:
            image_window_docks[channel] = dock.Dock(channel,
                                                    autoOrientation=False)
            image_window_docks[channel].setTitle(channel)
            image_window_docks[channel].showTitleBar()
            print(image_window_docks[channel].title())
            # image_window_docks[channel].setOrientation(o = 'vertical', force = True)
            # image_window_docks[channel].setStretch(x = 1000, y= 1000)
            image_display_dockArea.addDock(image_window_docks[channel],
                                           'bottom')
            image_window_docks[channel].addWidget(
                self.imageDisplayWindow[channel].widget)
            last_channel = channel

        # Add dock for the thresholded image
        thresholded_image_dock = dock.Dock('Thresholded',
                                           autoOrientation=False)
        image_display_dockArea.addDock(thresholded_image_dock, 'right',
                                       image_window_docks[last_channel])
        thresholded_image_dock.addWidget(
            self.imageDisplayWindow_ThresholdedImage.widget)

        #-----------------------------------------------------------------------------------------------
        # PDAF
        #-----------------------------------------------------------------------------------------------
        if TWO_CAMERA_PDAF:
            import control.core_PDAF as core_PDAF
            self.PDAFController = core_PDAF.PDAFController(
                self.trackingController)
            self.PDAFControllerWidget = widgets_tracking.PDAFControllerWidget(
                self.PDAFController)
            self.imageDisplayWindow['PDAF_image1'] = core.ImageDisplayWindow(
                key + ' Display', DrawCrossHairs=True)
            self.imageDisplayWindow['PDAF_image2'] = core.ImageDisplayWindow(
                key + ' Display', DrawCrossHairs=True)
            # add docked imaged display
            PDAF_image1_dock = dock.Dock('PDAF_image1', autoOrientation=False)
            image_display_dockArea.addDock(PDAF_image1_dock, 'bottom')
            PDAF_image1_dock.addWidget(
                self.imageDisplayWindow['PDAF_image1'].widget)
            PDAF_image2_dock = dock.Dock('PDAF_image2', autoOrientation=False)
            image_display_dockArea.addDock(PDAF_image2_dock, 'right',
                                           PDAF_image1_dock)
            PDAF_image2_dock.addWidget(
                self.imageDisplayWindow['PDAF_image2'].widget)
            # make connections
            self.streamHandler['DF1'].image_to_display.connect(
                self.PDAFController.register_image_from_camera_1)
            self.streamHandler['DF2'].image_to_display.connect(
                self.PDAFController.register_image_from_camera_2)
            self.PDAFController.signal_image1.connect(
                self.imageDisplayWindow['PDAF_image1'].display_image)
            self.PDAFController.signal_image2.connect(
                self.imageDisplayWindow['PDAF_image2'].display_image)

        #-----------------------------------------------------------------------------------------------
        # Volumetric Imaging
        #-----------------------------------------------------------------------------------------------
        import control.trigger_controller as trigger_controller
        import control.optotune_lens as optotune_lens
        if VOLUMETRIC_IMAGING:
            self.liquid_lens = optotune_lens.optotune_lens()
            if USE_SEPARATE_TRIGGER_CONTROLLER:
                if simulation:
                    self.trigger_controller = trigger_controller.TriggerController_Simulation(
                        TRIGGERCONTROLLER_SERIAL_NUMBER)
                else:
                    self.trigger_controller = trigger_controller.TriggerController(
                        TRIGGERCONTROLLER_SERIAL_NUMBER)
            else:
                self.trigger_controller = self.microcontroller
            self.volumetricImagingStreamHandler = core_volumetric_imaging.VolumetricImagingStreamHandler(
                self.trackingController,
                imaging_channel='volumetric imaging',
                rotate_image_angle=CAMERAS['volumetric imaging']
                ['rotate image angle'],
                flip_image=CAMERAS['volumetric imaging']['flip image'])
            self.VolumetricImagingImageSaver = core_volumetric_imaging.VolumetricImagingImageSaver(
                self.internal_state)
            self.volumetricImagingController = core_volumetric_imaging.VolumetricImagingController(
                self.camera['volumetric imaging'], self.trigger_controller,
                self.liquid_lens, self.volumetricImagingStreamHandler,
                self.VolumetricImagingImageSaver, self.internal_state)
            self.volumetricImagingWidget = widgets_volumetric_imaging.VolumetricImagingWidget(
                self.volumetricImagingController)
            self.streamHandler[
                'volumetric imaging'] = self.volumetricImagingStreamHandler
            self.imageSaver['volumetric imaging'].close()
            self.imageSaver[
                'volumetric imaging'] = self.VolumetricImagingImageSaver
            self.focusMeasureDisplayWindow = widgets_volumetric_imaging.FocusMeasureDisplayWindow(
            )
            self.focusMeasureDisplayWindow.show()
            self.imageArrayDisplayWindow = core_volumetric_imaging.ImageArrayDisplayWindow(
            )
            self.imageArrayDisplayWindow.show()
            self.volumetricImagingStreamHandler.packet_image_for_array_display.connect(
                self.imageArrayDisplayWindow.display_image)
            self.volumetricImagingStreamHandler.signal_focus_measure_plot.connect(
                self.focusMeasureDisplayWindow.plotWidget.plot)
            if simulation == False:
                # in simulation mode, do not change camera trigger mode (for now) or no new image would be delivered, as the trigger timer is in the livecontroller
                # to do: add a tigger timer in the simulation camera object for simulating hardware trigger
                self.volumetricImagingController.signal_trigger_mode.connect(
                    self.cameraSettingsWidget['volumetric imaging'].
                    set_trigger_mode)
            self.volumetricImagingStreamHandler.signal_defocus.connect(
                self.volumetricImagingWidget.display_defocus.display)
            self.streamHandler[
                'volumetric imaging'].signal_new_frame_received.connect(
                    self.liveController[channel].on_new_frame)
            self.streamHandler['volumetric imaging'].image_to_display.connect(
                self.imageDisplayWindow[channel].display_image)
            self.streamHandler[
                'volumetric imaging'].packet_image_to_write.connect(
                    self.imageSaver[channel].enqueue)
            self.imageSaver['volumetric imaging'].imageName.connect(
                self.trackingDataSaver.setImageName)
            self.streamHandler['volumetric imaging'].signal_fps_save.connect(
                self.recordingControlWidget.update_save_fps)

        #-----------------------------------------------------
        # Layout widgets
        #-----------------------------------------------------
        self.SettingsTab = QTabWidget()
        # self.SettingsTab.addTab(self.PID_Group_Widget, 'PID')
        if TWO_CAMERA_PDAF:
            self.SettingsTab.addTab(self.PDAFControllerWidget, 'PDAF')
        if VOLUMETRIC_IMAGING:
            self.SettingsTab.addTab(self.volumetricImagingWidget,
                                    'Volumetric Imaging')
        self.SettingsTab.addTab(self.navigationWidget, 'Stage Control')
        self.SettingsTab.addTab(self.ledMatrixControlWidget, 'LED Matrix')
        self.SettingsTab.addTab(self.plotWidget, 'Plots')

        layout_left = QVBoxLayout()
        layout_left.addWidget(self.liveSettings_Tab)
        layout_left.addWidget(self.trackingControlWidget)
        layout_left.addWidget(self.recordingControlWidget)
        layout_left.addStretch()

        layout_right = QVBoxLayout()
        layout_right.addWidget(self.SettingsTab)
        layout_right.addWidget(self.PID_Group_Widget)

        overall_layout = QHBoxLayout()
        overall_layout.addLayout(layout_left)
        overall_layout.addLayout(layout_right)

        # transfer the layout to the central widget
        self.centralWidget = QWidget()
        self.centralWidget.setLayout(overall_layout)
        self.setCentralWidget(self.centralWidget)

        #-----------------------------------------------------
        # Start all image-streams
        #-----------------------------------------------------
        for channel in self.imaging_channels:
            self.camera[channel].set_software_triggered_acquisition(
            )  #self.camera.set_continuous_acquisition()
            self.camera[channel].set_callback(
                self.streamHandler[channel].on_new_frame)
            self.camera[channel].enable_callback()
            self.camera[channel].start_streaming()
        self.image_window.show()
コード例 #4
0
    def __init__(self, *args, **kwargs):
        super().__init__(*args, **kwargs)

        # load objects
        self.camera = camera.Camera(sn=33910474,
                                    width=4000,
                                    height=3000,
                                    framerate=30,
                                    color=False)
        self.microcontroller = microcontroller.Microcontroller()

        self.configurationManager = core.ConfigurationManager()
        self.streamHandler = core.StreamHandler()
        self.liveController = core.LiveController(self.camera,
                                                  self.microcontroller,
                                                  self.configurationManager)
        self.navigationController = core.NavigationController(
            self.microcontroller)
        self.autofocusController = core.AutoFocusController(
            self.camera, self.navigationController, self.liveController)
        self.multipointController = core.MultiPointController(
            self.camera, self.navigationController, self.liveController,
            self.autofocusController, self.configurationManager)
        self.trackingController = core.TrackingController(
            self.microcontroller, self.navigationController)
        self.imageSaver = core.ImageSaver()
        self.imageDisplay = core.ImageDisplay()

        # open the camera
        # camera start streaming
        self.camera.open()
        self.camera.set_software_triggered_acquisition(
        )  #self.camera.set_continuous_acquisition()
        self.camera.set_callback(self.streamHandler.on_new_frame)
        self.camera.enable_callback()

        # load widgets
        self.cameraSettingWidget = widgets.CameraSettingsWidget(
            self.camera, self.liveController)
        self.liveControlWidget = widgets.LiveControlWidget(
            self.streamHandler, self.liveController, self.configurationManager)
        self.navigationWidget = widgets.NavigationWidget(
            self.navigationController)
        self.autofocusWidget = widgets.AutoFocusWidget(
            self.autofocusController)
        self.recordingControlWidget = widgets.RecordingWidget(
            self.streamHandler, self.imageSaver)
        self.trackingControlWidget = widgets.TrackingControllerWidget(
            self.streamHandler, self.trackingController)
        self.multiPointWidget = widgets.MultiPointWidget(
            self.multipointController, self.configurationManager)

        self.recordTabWidget = QTabWidget()
        self.recordTabWidget.addTab(self.recordingControlWidget,
                                    "Simple Recording")
        self.recordTabWidget.addTab(self.trackingControlWidget, "Tracking")
        self.recordTabWidget.addTab(self.multiPointWidget,
                                    "Multipoint Acquisition")

        # layout widgets
        layout = QGridLayout()  #layout = QStackedLayout()
        layout.addWidget(self.cameraSettingWidget, 0, 0)
        layout.addWidget(self.liveControlWidget, 1, 0)
        layout.addWidget(self.navigationWidget, 2, 0)
        layout.addWidget(self.autofocusWidget, 3, 0)
        layout.addWidget(self.recordTabWidget, 4, 0)

        # transfer the layout to the central widget
        self.centralWidget = QWidget()
        self.centralWidget.setLayout(layout)
        self.setCentralWidget(self.centralWidget)

        # load window
        self.imageDisplayWindow = core.ImageDisplayWindow()
        self.imageArrayDisplayWindow = core.ImageArrayDisplayWindow()
        self.imageDisplayWindow.show()
        self.imageArrayDisplayWindow.show()

        # make connections
        self.streamHandler.signal_new_frame_received.connect(
            self.liveController.on_new_frame)
        self.streamHandler.image_to_display.connect(self.imageDisplay.enqueue)
        self.streamHandler.packet_image_to_write.connect(
            self.imageSaver.enqueue)
        self.streamHandler.packet_image_for_tracking.connect(
            self.trackingController.on_new_frame)
        self.imageDisplay.image_to_display.connect(
            self.imageDisplayWindow.display_image
        )  # may connect streamHandler directly to imageDisplayWindow
        self.navigationController.xPos.connect(
            self.navigationWidget.label_Xpos.setNum)
        self.navigationController.yPos.connect(
            self.navigationWidget.label_Ypos.setNum)
        self.navigationController.zPos.connect(
            self.navigationWidget.label_Zpos.setNum)
        self.autofocusController.image_to_display.connect(
            self.imageDisplayWindow.display_image)
        # self.multipointController.image_to_display.connect(self.imageDisplayWindow.display_image)
        self.multipointController.signal_current_configuration.connect(
            self.liveControlWidget.set_microscope_mode)
        self.multipointController.image_to_display_multi.connect(
            self.imageArrayDisplayWindow.display_image)
        self.liveControlWidget.signal_newExposureTime.connect(
            self.cameraSettingWidget.set_exposure_time)
        self.liveControlWidget.signal_newAnalogGain.connect(
            self.cameraSettingWidget.set_analog_gain)
        self.liveControlWidget.update_camera_settings()